Marine oligosaccharide composition as well as preparation method and application thereof

Abstract

The invention discloses a marine oligosaccharide composition as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical compositions. The marine oligosaccharide composition is prepared from the following components in parts by weight: 20 to 60 parts of marine oligosaccharide extract, 5 to 20 parts of pleurotus citrinopileatus concentrated powder, 20 to 50 parts of L-alpha-choline glycerophosphate and 0 to 50 parts of maltodextrin. The composition can greatly improve the memory improvement effect of L-alpha-choline alfoscerate, and can effectively relieve fatigue, so that the composition has a good application prospect in the fields of human body memory recovery and fatigue rehabilitation.

Description

Technical Field

[0001] The present invention belongs to the technical field of pharmaceutical compositions, and in particular relates to a marine oligosaccharide composition and a preparation method and use thereof. Background Art

[0002] L-α-Glycerophosphocholine is a natural water-soluble intermediate of phospholipid metabolism, composed of choline, glycerol and phosphate. It belongs to the natural choline class and is widely present in the human body, especially the brain. It is a precursor for the synthesis of phosphatidylcholine and the neurotransmitter acetylcholine. This substance plays an important role in lipid metabolism, neuroprotection and cell signal transduction, and is essential for maintaining the normal function of the organism. In the body, L-α-Glycerophosphocholine is a cholinesterase inhibitor and a biosynthetic precursor of the neurotransmitter acetylcholine. As an important biologically active substance, L-α-Glycerophosphocholine has the functions of repairing impaired cognitive ability and enhancing brain activity. During exercise, L-α-Glycerophosphocholine can also increase muscle mass and improve overall metabolic activity by increasing the production of acute growth hormone and power output, thereby improving body composition. In addition, L-α-Glycerophosphocholine can also accelerate neurotransmitter transmission, improve the efficiency of neuromuscular connection, increase the contractility and endurance of skeletal muscle, thereby enhancing the body's exercise intensity, anti-fatigue, and having a positive effect on muscle growth. Currently, L-α-phosphoglycerate choline has been used to treat diseases or conditions associated with the aggregation of β-amyloid protein, Alzheimer's disease, stroke, improve blood-brain barrier function, improve blood-brain barrier transport of active agents across the blood-brain barrier, improve or maintain cognitive health, provide neuroprotection, promote neural development or promote brain metabolism, so that one or more of the aforementioned conditions are treated, improved or helped.

[0003] L-α-glycerophosphocholine has been approved as a new food ingredient, but its recommended intake is ≤600 mg / day (on a dry basis). According to relevant data, some special populations do not take enough L-α-glycerophosphocholine, which can not meet the effective dose, limiting the realization of its health effects. Therefore, how to improve the effect of L-α-glycerophosphocholine has become one of the current research hotspots and important topics. Summary of the invention

[0004] The present invention provides a marine oligosaccharide composition, which comprises the following components:

[0005] 20-60 parts of marine oligosaccharide extract, 5-20 parts of Pleurotus eryngii concentrated powder, 20-50 parts of L-α-glycerophosphorylcholine, and 0-50 parts of maltodextrin.

[0006] In a specific embodiment, the marine oligosaccharide composition comprises the following:

[0007] 60 parts of marine oligosaccharide extract, 10 parts of Pleurotus eryngii concentrated powder, and 30 parts of L-α-glycerophosphorylcholine.

[0008] In a specific embodiment, the marine oligosaccharide composition comprises the following:

[0009] 20 parts of marine oligosaccharide extract, 10 parts of Pleurotus eryngii concentrated powder, 30 parts of L-α-glycerophosphorylcholine, and 40 parts of maltodextrin.

[0010] In a specific embodiment, the marine oligosaccharide composition comprises the following:

[0011] 20 parts of marine oligosaccharide extract, 10 parts of Pleurotus eryngii concentrated powder, 50 parts of L-α-glycerophosphorylcholine, and 20 parts of maltodextrin.

[0012] In a specific embodiment, the marine oligosaccharide composition comprises the following:

[0013] 40 parts of marine oligosaccharide extract, 10 parts of Pleurotus eryngii concentrated powder, and 50 parts of L-α-glycerophosphorylcholine.

[0014] In a specific embodiment, the marine oligosaccharide composition comprises the following:

[0015] 25 parts of marine oligosaccharide extract, 5 parts of Pleurotus eryngii concentrated powder, 20 parts of L-α-glycerophosphorylcholine, and 50 parts of maltodextrin.

[0016] In the present invention, the marine oligosaccharide extract is prepared by the following method:

[0017] The agar-agar and staghorn herb are cleaned, roughly chopped, added with water, ground to obtain a homogenate, and the homogenate is broken; then an acidulant is added, softened, and the pH is adjusted to obtain a slurry; the slurry is sterilized and then filtered; the pH of the filtrate is adjusted, and a pH stabilizer is added; then a composite enzyme is added for enzymolysis; the enzymolysis solution is inactivated; then filtering and freeze-drying are performed to obtain a marine oligosaccharide extract.

[0018] In the above-mentioned method for preparing the marine oligosaccharide extract, the mass ratio of the agar-agar and the chordate is selected from (2 to 3):

[0019] (1~2); preferably 3:2.

[0020] In the above-mentioned method for preparing the marine oligosaccharide extract, each raw material is selected from the following amounts:

[0021] 3-5 parts of agar-agar and staghorn, 15-50 parts of water, 0.1-0.5 parts of acidulant, 0.01-0.5 parts of pH stabilizer, and 3-5 parts of complex enzyme.

[0022] In a specific embodiment, in the method for preparing the marine oligosaccharide extract, each raw material is selected from the following amounts:

[0023] 5 parts of agar-agar and staghorn, 50 parts of water, 0.1 parts of acidulant, 0.01 parts of pH stabilizer, and 5 parts of complex enzyme.

[0024] In the above method for preparing the marine oligosaccharide extract, the amount of water used is 5 to 10 times the total amount of Gelidium agar and Staghornis salsa.

[0025] In the above-mentioned method for preparing the marine oligosaccharide extract, the cell wall breaking condition is selected from normal temperature and high pressure cell wall breaking, and the pressure is 100-1000MPa; preferably 500MPa.

[0026] In the above-mentioned method for preparing the marine oligosaccharide extract, the acidulant is concentrated sulfuric acid and anhydrous oxalic acid, and the mass ratio of the two is selected from (2-3):(1-2); preferably 2:1.

[0027] In the above method for preparing the marine oligosaccharide extract, the softening condition is selected from: softening at 50-70°C for 1-2 hours; preferably softening at 50°C for 1 hour.

[0028] In the method for preparing the marine oligosaccharide extract, the pH of the slurry is 6-7; the pH of the filtrate is 6.5-8.5.

[0029] In the above-mentioned method for preparing marine oligosaccharide extract, the pH stabilizer is one or more of sodium citrate, potassium citrate and ethylenediaminetetraacetic acid.

[0030] In the preparation method of the above marine oligosaccharide extract, the complex enzyme is composed of agarase, cellulase, pectinase, xylanase, mannanase, β-glucanase and alginate lyase, and the mass ratio thereof is selected from (1 to 5): (1 to 5):

[0031] (1~3):(1~2):(1~3):(1~2):(1~5); preferably 3:3:1:1:1:1:2.

[0032] In the above-mentioned method for preparing the marine oligosaccharide extract, the enzymatic hydrolysis conditions are selected from: enzymatic hydrolysis at 30-50° C. for 6-12 h; preferably: enzymatic hydrolysis at 35° C. for 6 h.

[0033] In the above-mentioned method for preparing the marine oligosaccharide extract, the enzyme inactivation condition is selected from: heating at 85-95°C for 15-25 min; preferably: heating at 95°C for 20 min.

[0034] The present invention provides the use of the marine oligosaccharide composition in preparing a medicine having the effects of improving memory and / or relieving fatigue.

[0035] In the present invention, the polymer range of the marine oligosaccharide extract is 2 to 8, the agar oligosaccharide content is more than 30%, and the brown algae oligosaccharide content is more than 20%; the ergothioneine content in the Pleurotus eryngii concentrated powder is more than 5% (mass fraction).

[0036] The beneficial effects of the present invention are:

[0037] The marine oligosaccharide composition of the present invention consists of marine oligosaccharide extract, Pleurotus eryngii concentrated powder and L-α-glycerophosphorylcholine. The composition can greatly improve the effect of L-α-glycerophosphorylcholine on improving memory, and can also effectively relieve fatigue, thereby showing good application prospects in the fields of human memory recovery and fatigue rehabilitation. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 A maze swim lane diagram. DETAILED DESCRIPTION

[0039] In the present invention, the concentrated powder of Pleurotus eryngii is purchased from LS CORPORATION of Japan, and the ergothioneine content thereof is above 5% (mass fraction).

[0040] In the present invention, the marine oligosaccharide extract is prepared by the following method:

[0041] Agar agar and staghorn are used as raw materials, wherein the mass ratio of agar agar to staghorn is 3:2; 50 g of agar agar and staghorn are cleaned, roughly chopped, 10 times the weight of water is added, and ground to obtain a homogenate with a particle size of 60 meshes, and then the homogenate is subjected to room temperature ultra-high pressure (500 MPa) wall breaking; then 1 g of an acidifier (concentrated sulfuric acid: anhydrous oxalic acid = 2:1) is added, softened at 50°C for 1 hour, and an alkali solution is used to adjust the pH to 6-7 to obtain a slurry; the slurry is placed in a high-temperature and high-pressure sterilizer for treatment at 115°C for 30 min, and then filtered; the pH of the filtrate is adjusted to 6.5-8.5, and 0.1g of sodium citrate (pH stabilizer) is added; then 50g of complex enzyme is added, and enzymolysis is carried out at 35°C for 6h; the complex enzyme includes agarase, cellulase, pectinase, xylanase, mannanase, β-glucanase, and alginate lyase, and the mass ratio thereof is 3:3:1:1:1:1:2; the enzymatic hydrolyzate is heated at 95°C for 20min to inactivate the enzyme; then the slurry is filtered and freeze-dried to obtain a marine oligosaccharide extract.

[0042] The prepared marine oligosaccharide extract has a polymerization degree ranging from 2 to 8, agar oligosaccharide content of more than 30%, and brown algae oligosaccharide content of more than 20%.

[0043] In the present invention, maltodextrin is only used as a conventional additive for food and medicine, and does not have the functions and effects of improving memory or relieving fatigue, and will not affect the functions and effects of other drug components.

[0044] Other materials used in the present invention, if not otherwise stated, can be obtained through commercial channels. Other terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art. The present invention is further described in detail below in conjunction with specific examples and with reference to data. The following examples are only for illustrating the present invention, and are not intended to limit the scope of the present invention in any way.

[0045] Example 1

[0046] A marine oligosaccharide composition has the following proportions: 60 g of marine oligosaccharide extract, 10 g of Pleurotus eryngii concentrated powder, and 30 g of L-α-glycerophosphorylcholine.

[0047] Example 2

[0048] A marine oligosaccharide composition has the following proportions: 20 g of marine oligosaccharide extract, 10 g of Pleurotus eryngii concentrated powder, 30 g of L-α-glycerophosphorylcholine, and 40 g of maltodextrin.

[0049] Example 3

[0050] A marine oligosaccharide composition has the following proportions: 20 g of marine oligosaccharide extract, 10 g of Pleurotus eryngii concentrated powder, 50 g of L-α-glycerophosphorylcholine, and 20 g of maltodextrin.

[0051] Example 4

[0052] A marine oligosaccharide composition has the following proportions: 40 g of marine oligosaccharide extract, 10 g of Pleurotus eryngii concentrated powder, and 50 g of L-α-glycerophosphorylcholine.

[0053] Example 5

[0054] A marine oligosaccharide composition has the following proportions: 25 g of marine oligosaccharide extract, 5 g of Pleurotus eryngii concentrated powder, 20 g of L-α-glycerophosphorylcholine, and 50 g of maltodextrin.

[0055] Comparative Example 1

[0056] A L-α-glycerophosphorylcholine composition has the following formula: 30 g of L-α-glycerophosphorylcholine and 70 g of maltodextrin.

[0057] Comparative Example 2

[0058] A L-α-glycerophosphorylcholine composition has the following formula: 50 g of L-α-glycerophosphorylcholine and 50 g of maltodextrin.

[0059] Comparative Example 3

[0060] A marine oligosaccharide composition has the following proportions: 20 g of marine oligosaccharide extract, 10 g of Pleurotus eryngii concentrated powder, and 70 g of maltodextrin.

[0061] Comparative Example 4

[0062] A marine oligosaccharide composition has the following proportions: 60g of marine oligosaccharide extract, 10g of Pleurotus eryngii concentrated powder, and 30g of maltodextrin.

[0063] 1. Assist in improving memory

[0064] 1. Dark avoidance experiment

[0065] (1) Principle

[0066] Taking advantage of the dark-loving habits of mice, a device was designed. Half of the room was dark and the other half was bright, connected by a small hole in the middle. The bottom of the dark room was covered with an electrified copper grid and connected to a timer, which could automatically record the incubation period. When the mouse entered the dark room, it was shocked and the timer stopped automatically.

[0067] (2) Instruments

[0068] Dark-avoiding device: This device is divided into two chambers, light and dark. The light chamber is 12cm×4.5cm in size, with a 40w tungsten filament hanging about 20cm above it. The dark chamber is larger, 17cm×4.5cm in size, with a round hole of about 3cm in diameter between the two chambers. The bottom of both chambers is covered with copper grids. The copper grid in the middle of the bottom of the dark chamber can be electrified, and the intensity of the electric shock can be selected at will on a knob. Generally, a voltage of 40v is used. The dark chamber is connected to a timer, which can automatically record the time of the incubation period.

[0069] (3) Experimental methods

[0070] Adult male mice (18-22 g) were divided into 9 groups, each with 10 mice. The test sample was administered at 500 mg / kg for 30 days.

[0071] The training started the next day after the last sample was given. During the experiment, the mouse was placed in the light chamber with its face facing away from the hole, and the timer was started at the same time. The animal passed through the hole and entered the dark chamber and received an electric shock. The timer automatically stopped, and the mouse was taken out. The time required for each mouse from being placed in the light chamber to entering the dark chamber and receiving an electric shock was recorded. This was the latency period. The training lasted for 5 minutes, and the number of electric shocks within 5 minutes was recorded. The test was repeated 24 hours or 48 hours later, and the latency period for each animal to enter the dark chamber and the number of electric shocks within 5 minutes were recorded. The percentage of animals that entered the dark chamber within 5 minutes (wrong response) was calculated.

[0072] (4) Data processing and result determination

[0073] The number of times the animals entered the dark room within 5 minutes and the number of animals entering the dark room within 5 minutes are both count data and can be calculated using the chi-square test. 2 Test: When the total number of cases in the four-cell table is less than 40, or the total number of cases is equal to or greater than 40 but the theoretical number is equal to or less than 1, the exact probability method should be used instead.

[0074] If the latency of the mice in the test sample group to enter the dark room is significantly longer than that in the control group, the number of errors in entering the dark room within 5 minutes or the number of animals entering the dark room within 5 minutes is less than that in the control group, and the difference is significant, any one of the above three indicators is positive, the experiment can be judged to be positive.

[0075] 2. Water maze experiment

[0076] (1) Experimental methods

[0077] Adult male mice (18-22 g) were divided into 9 groups, each with 10 mice. The test sample was administered at 500 mg / kg for 30 days.

[0078] Samples were given continuously for 30 days, and training began the day after the last sample was given. Samples were given once a day during the training period. Figure 1 ) The water depth was 9 cm and the water temperature was about 20°C (≮15°C). The training time of mice was limited to 2 min, and mice that did not reach the end point within 2 min were counted as 2 min.

[0079] Before the first training, place the mouse near the ladder and let it climb up automatically 3 times. Before each subsequent training, place the mouse near the ladder with its back facing the stairs and let it climb up automatically once. The experiment is carried out in stages, and the distance is gradually lengthened depending on the animal's learning performance. During the first training, a baffle is used to block the dead end at point A, and training begins at point A, recording the time it takes to reach the end point from point A. During the second training, the distance is lengthened, starting from point B. This distance is trained about 3 times, and the distance is extended again after more than 80% of the animals reach the end point within 2 minutes. The time required for each mouse to reach the end point from point B and the number of errors that occur are recorded separately (entering any blind end once is counted as an error). The last test is carried out from the starting point, and the mouse is placed at the starting point. The time required to reach the end point from the starting point and the number of errors that occur are recorded. During each training, for mice that have not reached the end point within 2 minutes, they should be guided to the end point and come up the stairs at the end point to achieve the purpose of training. The head should face the starting point during each training or test. Finally, the total number of errors in 5 training and testing sessions, the total time to reach the end point, and the total number of animals that reached the end point within 2 min (percentage) were calculated for each group of animals. Extinction experiments were performed from the starting point at different times 5 days after stopping training.

[0080] 3. Test results

[0081] The test results are shown in Table 1 and Table 2:

[0082] Table 1 Effects of samples on the dark avoidance test of mice

[0083]

[0084] Table 2 Effects of samples on mouse water maze test

[0085]

[0086]

[0087] Compared with the control group, the time taken by the test group, the number of errors before reaching the end point, and the number of errors in Example 1, Example 2, Example 3, Example 4, Example 5, Comparative Example 1, and Comparative Example 2 to reach the end point were significantly less than those in the control group, or the number of animals reaching the end point within 2 minutes was significantly more than that in the control group, or the latency time was significantly higher than that in the control group, and the difference was statistically significant. If any of the indicators is positive, it can be judged that the sample has the effect of improving memory.

[0088] From the results of Example 1 and Comparative Example 1, and Example 3 and Comparative Example 2, it can be seen that the marine oligosaccharide extract and the concentrated powder of Pleurotus eryngii can enhance the effect of L-α-glyophosphorylcholine on improving memory. From the results of Example 5 and Comparative Example 2, it can be seen that under the same dosage of the drug, the effect of the composition on improving memory is much higher than that of L-α-glyophosphorylcholine, which further illustrates that the marine oligosaccharide extract and the concentrated powder of Pleurotus eryngii can enhance the effect of L-α-glyophosphorylcholine on improving memory. From the results of Example 1 and Comparative Example 4, and Example 3 and Comparative Example 3, it can be seen that the combination of L-α-glyophosphorylcholine with marine oligosaccharide extract and concentrated powder of Pleurotus eryngii has a better effect on improving memory.

[0089] 2. Relieve physical fatigue

[0090] 1. Weighted swimming test

[0091] (1) Inspection principle

[0092] The improvement of exercise endurance is the most direct manifestation of enhanced anti-fatigue ability, and the length of swimming time can reflect the degree of exercise fatigue of the animal.

[0093] (2) Instruments and equipment

[0094] Swimming box (approximately 50cm×50cm×40cm), electronic balance, lead sheet.

[0095] (3) Experimental methods

[0096] Experimental animals: Pure strain adult male mice, weighing 18-22 g.

[0097] Dosage and administration time of the test sample: 500 mg / kg of the test sample. The administration time of the test sample is 30 days.

[0098] Experimental steps:

[0099] 30 minutes after the last administration of the test sample (gavage is not necessary on the day of the alcohol sample test), place the mouse with a lead sheet of 5% of its body weight at the base of its tail in a swimming box. The water depth should be no less than 30 cm and the water temperature should be 25℃±1.0℃. The time from the start of swimming to the death of the mouse is recorded, i.e., the weighted swimming time of the mouse.

[0100] If the weighted swimming time of the test sample group is significantly longer than that of the control group, and the difference is significant, the experimental result can be judged to be positive.

[0101] 2. Blood lactate determination

[0102] (1) Instruments and reagents

[0103] Instruments: micropipette, constant temperature water bath, electric water tank, spectrophotometer.

[0104] Reagent: 4% CuSO 4 , concentrated sulfuric acid (AR), 1% NaF solution.

[0105] Protein precipitant: Take one part of 10% sodium tungstate and one part of 1 / 3 mol / L sulfuric acid by volume, and then mix them with 28 parts of distilled water.

[0106] Precipitant-NaF mixed solution: Take 3 parts of precipitant and 1 part of 1% NaF by volume and mix them.

[0107] 1.5% p-hydroxybiphenyl solution: weigh 1.5g p-hydroxybiphenyl and dissolve it in 100mL hot 0.5% NaOH (can be stored for half a year).

[0108] Lactic acid standard stock solution (1 g / L): Weigh 106.6 mg lithium lactate or 171 mg calcium lactate and dilute to 100 mL with 10% trichloroacetic acid (can be stored at room temperature for half a year).

[0109] Lactic acid standard application solution (0.01g / L): Accurately pipette 1.0mL of lactic acid standard stock solution and dilute to 100mL. This solution must be prepared before use.

[0110] (2) Experimental methods

[0111] Experimental animals: Pure strain adult male mice, weighing 18-22 g.

[0112] Dosage and administration time of the test sample: 500 mg / kg of the test sample. The administration time of the test sample is 30 days.

[0113] Experimental steps:

[0114] Preparation of high blood lactate model and preparation of blood samples: Blood was collected 30 minutes after the last sample was given, and then swimming without weight in water at 30°C for 10 minutes before stopping. Blood was collected from the inner canthus using a capillary tube.

[0115] Determination steps: Add 0.48mL of 1% NaF solution to a 5mL test tube, accurately pipette 20μL of whole blood and add it to the bottom of the test tube. Wash the micropipette several times with the test tube supernatant, then add 1.5mL of protein precipitant, shake and mix, centrifuge at 3000r / min for 10min, take the supernatant, and operate according to Table 3.

[0116] Table 3 Standard dosage of reagents in each test tube

[0117]

[0118] After completing the above steps, shake well and place in a 30°C water bath for 30 minutes (shake once every 10 minutes). Take it out and heat it in a boiling water bath for 90 seconds, take it out and cool it to room temperature, compare the color at a wavelength of 560nm with a 5mm optical path cuvette, and adjust the blank tube to zero.

[0119] Calculation of blood lactate content: blood lactate content (mg / L) = A 测定管 / A 标准管 ×100×10.

[0120] (3) Data processing and result determination:

[0121] Determination of experimental results: The area under the blood lactate curve at three time points is used for determination. If the area under any experimental group is smaller than that under the control group and the difference is significant, the experimental result can be determined to be positive.

[0122] Calculation method of area under the blood lactate curve:

[0123] The area under the blood lactate curve = 1 / 2 × (blood lactate value before swimming + blood lactate value 0 min after swimming) × 10 + 1 / 2 × (blood lactate value 0 min after swimming + blood lactate value 20 min after resting after swimming) × 20 = 5 × (blood lactate value before swimming + 3 × blood lactate value 0 min after swimming + 2 × blood lactate value 20 min after resting after swimming).

[0124] 3. Test results

[0125] The test results are shown in Table 4 and Table 5:

[0126] Table 4 Effects of samples on weight-bearing swimming time of mice

[0127]

[0128] Table 5 Effects of samples on blood lactate in mice

[0129]

[0130]

[0131] From the above results, it can be seen that the weighted swimming time of the experimental group is higher than that of the control group, and the area under the blood lactate curve is lower than that of the control group. The weighted swimming time and the area under the blood lactate curve of Example 1, Example 2, Example 3, Example 4, Example 5, and Comparative Example 4 are significantly different from those of the control group (P>0.05), indicating that the samples can significantly relieve the physical fatigue of mice.

[0132] From the comparison results of Example 1 and Example 2, and Example 3 and Example 4, it can be seen that the higher the content of the oligosaccharide extract, the better the effect of relieving physical fatigue of mice.

[0133] From the results of Example 1 and Comparative Example 4, and Example 3 and Comparative Example 3, it can be seen that L-α-glyophosphorylcholine is better in relieving physical fatigue of mice when combined with oligosaccharide extract and Pleurotus eryngii concentrated powder. From the results of Example 5 and Comparative Example 2, it can be seen that under the same dosage of the drug, the effect of the composition in relieving physical fatigue is much higher than that of L-α-glyophosphorylcholine alone. This shows that marine oligosaccharide extract and Pleurotus eryngii concentrated powder can enhance the effect of L-α-glyophosphorylcholine on relieving fatigue.

[0134] In summary, the marine oligosaccharide composition of the present invention can significantly improve the memory of mice and has the effect of relieving fatigue.

[0135] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

Claims

1. A marine oligosaccharide composition, characterized in that: The composition is as follows: 20-60 parts of marine oligosaccharide extract, 5-20 parts of Pleurotus eryngii concentrated powder, 20-50 parts of L-α-glycerophosphorylcholine, and 0-50 parts of maltodextrin.

2. The marine oligosaccharide composition according to claim 1, characterized in that The marine oligosaccharide extract is prepared by the following method: The agar-agar and staghorn are cleaned, roughly chopped, added with water, ground to obtain a homogenate, and the homogenate is broken; then an acidifier is added, softened, and the pH is adjusted to obtain a slurry; the slurry is sterilized and filtered; the pH of the filtrate is adjusted, and a pH stabilizer is added; then a composite enzyme is added for enzymolysis; The enzymatic hydrolysate is inactivated, filtered and freeze-dried to obtain the marine oligosaccharide extract.

3. The marine oligosaccharide composition according to claim 2, characterized in that The mass ratio of the agar-agar and chordate is selected from (2-3): (1-2).

4. The marine oligosaccharide composition according to claim 2, characterized in that The raw materials are selected from the following quantities: 3-5 parts of agar-agar and staghorn, 15-50 parts of water, 0.1-0.5 parts of acidulant, 0.01-0.5 parts of pH stabilizer, and 3-5 parts of complex enzyme.

5. The marine oligosaccharide composition according to claim 2, characterized in that: The conditions for breaking the wall are selected from normal temperature and high pressure breaking, and the pressure is 100-1000MPa.

6. The marine oligosaccharide composition according to claim 2, characterized in that The acidulant is concentrated sulfuric acid and anhydrous oxalic acid, and the mass ratio of the two is selected from (2-3): (1-2); and / or, the softening condition is selected from: softening at 50-70° C. for 1-2 hours.

7. The marine oligosaccharide composition according to claim 2, characterized in that The pH of the slurry is 6-7; the pH of the filtrate is 6.5-8.

5.

8. The marine oligosaccharide composition according to claim 2, characterized in that: The pH stabilizer is one or more of sodium citrate, potassium citrate and ethylenediaminetetraacetic acid.

9. The marine oligosaccharide composition according to claim 2, characterized in that: The complex enzyme is composed of agarase, cellulase, pectinase, xylanase, mannanase, β-glucanase and alginate lyase, and the mass ratio thereof is selected from (1-5):(1-5):(1-3):(1-2):(1-3):(1-2):(1-5); and / or the enzymatic hydrolysis conditions are selected from: enzymatic hydrolysis at 30-50°C for 6-12h; and / or the enzyme inactivation conditions are selected from: heating at 85-95°C for 15-25min.

10. Use of the marine oligosaccharide composition according to any one of claims 1 to 9 in the preparation of a drug having the effects of improving memory and / or relieving fatigue.

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