Composition for resisting ultraviolet induced inflammation and application thereof

Abstract

The invention discloses an anti-ultraviolet induced inflammation composition and application thereof, and the anti-ultraviolet induced inflammation composition comprises turmeric extract, epigallocatechin gallate and burdock extract; wherein the weight part of the turmeric extract is 2-10 parts, the weight part of the epigallocatechin gallate is 1-5 parts, and the weight part of the burdock extract is 1 part. The invention provides a composition for resisting ultraviolet-induced inflammation. According to the composition, generation of ultraviolet-induced inflammatory factors is inhibited through synergism of turmeric, EGGC and burdock.

Description

Technical Field

[0001] The invention belongs to the technical field of functional compositions, and in particular relates to an anti-ultraviolet induced inflammation composition and application thereof. Background Art

[0002] Ultraviolet radiation can cause skin tissue to produce a variety of inflammatory factors, which in turn induce changes in the internal structure of skin tissue and cause damage to cells and tissues. IL-6 is closely related to the growth and differentiation of the skin. It can play a role in cell growth and differentiation, activate the maturation of NK cells and hematopoietic stem cells, stimulate T cell chemokines to infiltrate the epidermis, and participate in regulating immune responses, hematopoiesis, acute phase responses, and inflammatory responses.

[0003] TNF-α is one of the cytokine network centers, which can lead to the release of multiple cytokines and then cause cell apoptosis, which can lead to inflammatory reactions in the skin.

[0004] Turmeric has been traditionally used in Indian and Chinese medicine as a dye, spice and anti-inflammatory. Curcumin, the active ingredient in turmeric, has a variety of health benefits including antioxidant, anti-inflammatory, anti-cancer, anti-diabetic, hepatoprotective, anti-allergic, anti-dermatophyte and neuroprotective.

[0005] EGCG is the main catechin found in green tea. The possible health benefits of green tea and EGCG include antioxidant effects, cancer chemoprevention, improved cardiovascular health, promotion of weight loss, protection of the skin from ionizing radiation damage, etc.

[0006] Burdock and its bioactive metabolites have shown a variety of pharmacological effects in vitro and in vivo, including antimicrobial, antiobesity, antioxidant, anticancer, anti-inflammatory, antidiabetic, antiallergic, antiviral, gastroprotective, hepatoprotective, and neuroprotective effects.

[0007] Currently, there are no reports on the synergistic effects of turmeric, EGCG, and burdock components against UV-induced inflammation. Summary of the invention

[0008] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0009] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0010] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide an anti-ultraviolet induced inflammation composition.

[0011] In order to solve the above technical problems, the present invention provides the following technical solutions: an anti-ultraviolet induced inflammation composition, comprising a turmeric extract, epigallocatechin gallate and a burdock extract;

[0012] The turmeric extract is 2 to 10 parts, the epigallocatechin gallate is 1 to 5 parts, and the burdock extract is 1 part.

[0013] As a preferred embodiment of the anti-ultraviolet induced inflammation composition of the present invention, the turmeric extract is 4.68-10 parts, the epigallocatechin gallate is 1-4 parts, and the burdock extract is 1 part.

[0014] As a preferred embodiment of the anti-ultraviolet induced inflammation composition of the present invention, the content of curcumin in the turmeric extract is ≥ 1%.

[0015] As a preferred embodiment of the anti-ultraviolet induced inflammation composition of the present invention, the epigallocatechin gallate is derived from green tea extract.

[0016] As a preferred embodiment of the anti-ultraviolet induced inflammation composition of the present invention, the purity of the epigallocatechin gallate is ≥94%.

[0017] Another object of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing an anti-ultraviolet induced inflammation composition, comprising uniformly mixing turmeric extract, epigallocatechin gallate and burdock extract to prepare the composition.

[0018] Another object of the present invention is to overcome the deficiencies in the prior art and provide an anti-ultraviolet induced inflammation composition for use in the preparation of medicines, health foods and foods for reducing the level of skin inflammatory factor TNF-α.

[0019] Another object of the present invention is to overcome the deficiencies in the prior art and provide an anti-ultraviolet induced inflammation composition for use in the preparation of drugs, health foods, and foods for reducing the level of skin inflammatory factor IL-6.

[0020] Another object of the present invention is to overcome the deficiencies in the prior art and provide an anti-ultraviolet induced inflammation composition for use in the preparation of food, health food, and medicine.

[0021] As a preferred embodiment of the application of the present invention, the food and health food include drinks, jellies, solid drinks, candies, soft capsules, hard capsules, tablets and biscuits.

[0022] Beneficial effects of the present invention:

[0023] (1) The present invention provides an anti-ultraviolet induced inflammation composition, wherein the composition synergistically inhibits the generation of inflammatory factors induced by ultraviolet (UV) rays through turmeric, EGGC, and burdock.

[0024] (2) The present invention optimizes the ratio of turmeric, EGCG and burdock. When turmeric is 2 to 10 parts, EGCG is 1 to 5 parts and burdock is 1 part, HaCAT cell experiments have verified that it can achieve a more significant inhibition of ultraviolet-induced inflammatory factor production through synergistic effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0026] Figure 1 This is a graph showing the results of IL-6 level detection in different experimental groups in the examples of the present invention.

[0027] Figure 2 1 is a graph showing the results of detecting TNF-α levels in different experimental groups in the examples of the present invention. DETAILED DESCRIPTION

[0028] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0029] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0031] In the embodiments of the present invention, the turmeric extract (1% curcumin) is a common commercial product; the burdock root extract and epigallocatechin gallate (EGCG, purity ≥ 94%) are both common commercial products.

[0032] The compositions prepared in the examples and comparative examples of the present invention are the test objects;

[0033] The contents of inflammatory factors IL-6 and TNF-α in HaCAT cells were measured to evaluate the anti-ultraviolet induced inflammatory effect of the composition;

[0034] The total experimental concentration was 15.625 μg / ml.

[0035] The specific experimental steps are as follows:

[0036] (1) Cultivation of human immortalized keratinocytes (HaCaT)

[0037] HaCaT cells are derived from normal skin tissue of a 62-year-old male. After in vitro culture, they spontaneously transform into immortalized human keratinocytes and are widely used in skin biology and differentiation research.

[0038] Culture the cells in a humidified incubator at 37°C with 5% CO2 using complete culture medium; replace the culture medium every 3 days and subculture at a ratio of 1:2-1:3 when the cell density reaches 90%.

[0039] (2) Effects of samples on inflammatory factors produced by long-wave ultraviolet (UVA) damage

[0040] The number of cells in logarithmic growth phase was adjusted to 7.5×10 4 The cells / mL were inoculated into 24-well plates, 1 mL per well, and cultured in a 37°C, 5% CO2 incubator for 24 h.

[0041] The cells were randomly divided into control group, model group and sample treatment group.

[0042] Before UVA irradiation, the sample treatment group was incubated with materials of appropriate concentration for 24 h, and the control group and the model group were incubated with culture medium, wherein the total concentration of the materials was 15.625 μg / ml, and the material ratios were shown in Table 1.

[0043] After incubation for 24 h, the culture medium was discarded and 200 μL PBS was added. The model group and sample treatment group were irradiated with 30 J UVA, while the control group was not irradiated with UVA.

[0044] After irradiation, 1 mL of sample solution of appropriate concentration was added to the sample group, and the same amount of culture medium was added to the control group and model group, and incubated overnight;

[0045] Discard the culture medium, wash with PBS 1-2 times, add 300 μL PBS to each well, place the well plate at -80°C, lyse the cells by freeze-thaw method (limited by the kit, if other kits are used, other methods can be used to collect proteins), and collect the solution;

[0046] Centrifuge at 5000 rpm for 10 min at 4°C and collect the supernatant;

[0047] The protein concentration in the supernatant was detected by BCA kit and adjusted to 10 μg / mL with PBS. The TNF-α and IL-6 contents in the samples were detected by Elisa kit.

[0048] Example 1

[0049] According to the weight ratio, 2 parts of turmeric extract, 1 part of EGCG and 1 part of burdock root extract were mixed evenly to obtain an anti-ultraviolet induced inflammation composition.

[0050] Example 2

[0051] According to the weight ratio, 2 parts of turmeric extract, 5 parts of EGCG and 1 part of burdock root extract were mixed evenly to obtain an anti-ultraviolet induced inflammation composition.

[0052] Example 3

[0053] 4.68 parts of turmeric extract, 1.5 parts of EGCG and 1 part of burdock root extract were mixed evenly according to the weight ratio to obtain an anti-ultraviolet induced inflammation composition.

[0054] Example 4

[0055] According to the weight ratio, 10 parts of turmeric extract, 1 part of EGCG and 1 part of burdock root extract were mixed evenly to obtain an anti-ultraviolet induced inflammation composition.

[0056] Example 5

[0057] 10 parts of turmeric extract, 5 parts of EGCG and 1 part of burdock root extract were mixed evenly according to the weight ratio to obtain an anti-ultraviolet induced inflammation composition.

[0058] Comparative Example 1

[0059] According to the weight ratio, 1 part of turmeric extract, 5 parts of EGCG and 1 part of burdock root extract were mixed evenly to obtain an anti-ultraviolet induced inflammation composition.

[0060] Comparative Example 2

[0061] According to the weight ratio, 2 parts of turmeric extract, 10 parts of EGCG and 1 part of burdock root extract were mixed evenly to obtain an anti-ultraviolet induced inflammation composition.

[0062] Comparative Example 3

[0063] According to the weight percentage, 4.68 parts of turmeric extract and 2.5 parts of EGCG were mixed evenly to obtain an anti-ultraviolet induced inflammation composition.

[0064] Comparative Example 4

[0065] 4.68 parts of turmeric extract and 2.5 parts of burdock root extract were mixed uniformly according to weight to obtain an anti-ultraviolet induced inflammation composition.

[0066] Comparative Example 5

[0067] 1.5 parts of EGCG and 1 part of burdock root extract were mixed evenly according to weight to obtain an anti-ultraviolet induced inflammation composition.

[0068] Table 1 Components of the anti-ultraviolet induced inflammation composition

[0069]

[0070]

[0071] The test results are shown in Table 2.

[0072] Table 2

[0073] IL-6 production (pg / mg protein) TNF-α production (pg / mgprotein) Example 1 392.72 109.80 Example 2 411.97 112.04 Example 3 251.97 60.67 Example 4 428.95 112.38 Example 5 432.20 114.09 Comparative Example 1 562.33 133.67 Comparative Example 2 569.83 134.34 Comparative Example 3 638.33 155.01 Comparative Example 4 645.33 155.34 Comparative Example 5 654.83 160.01

[0074] Result analysis: According to Table 2, the inflammatory factor level of Example 3 is the lowest (IL-6: 251.97 pg / mg protein, TNF-α: 60.67 pg / mg protein), which is lower than that of Examples 1, 2, 4, and 5, and also lower than that of Comparative Examples 1-5.

[0075] Figure 1 The IL-6 levels in different experimental groups are shown. The total concentrations of each experimental group were consistent, with mean ± SD, n = 3. One-way ANOVA was used for difference analysis. Different letters in the superscript indicate significant differences (P < 0.05). Figure 1 It can be seen that compared with the control group, the IL-6 content in the model group was significantly increased, while the anti-ultraviolet induced inflammatory composition treatment group could significantly reduce the IL-6 content (the IL-6 results of Examples 1-5 and Comparative Examples 1-5 were significantly different from the IL-6 results of the model group), among which the IL-6 results of Examples 1-5 were significantly different from the IL-6 results of Comparative Examples 1-5, and the IL-6 results of Example 3 were significantly different from the IL-6 results of Examples 1-2 and Examples 4-5).

[0076] Figure 2 The TNF-α levels in different experimental groups are shown. The total concentrations in each experimental group were consistent, with mean ± SD, n = 3. One-way ANOVA was used for difference analysis. Different letters in the superscript indicate significant differences (P < 0.05). Figure 2It can be seen that compared with the control group, the TNF-α content in the model group was significantly increased, while the groups treated with the anti-ultraviolet induced inflammation composition could significantly reduce the TNF-α content (the TNF-α results of Examples 1-5 and Comparative Examples 1-5 were significantly different from the TNF-α results of the model group), among which the TNF-α results of Examples 1-5 were significantly different from the TNF-α results of Comparative Examples 1-5, and the TNF-α results of Example 3 were significantly different from the TNF-α results of Examples 1-2 and Examples 4-5).

[0077] The combination ratio of the three materials in the anti-ultraviolet induced inflammation composition can synergistically inhibit the level of inflammatory factors in HaCaT cells.

[0078] The present invention belongs to the technical field of functional compositions, and relates to the application of a composition in the function of resisting ultraviolet-induced inflammation. The composition can be used as a main component in anti-ultraviolet-inflammatory foods or health foods, and can be made into beverages, jellies, solid beverages, candies, or soft and hard capsules, tablets, and biscuits.

[0079] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the present invention.

Claims

1. An anti-ultraviolet induced inflammation composition, characterized in that: These include, turmeric extract, epigallocatechin gallate, and burdock extract; The turmeric extract is 2 to 10 parts, the epigallocatechin gallate is 1 to 5 parts, and the burdock extract is 1 part.

2. The anti-ultraviolet induced inflammation composition according to claim 1, characterized in that: The turmeric extract is 4.68 to 10 parts, the epigallocatechin gallate is 1 to 4 parts, and the burdock extract is 1 part.

3. The anti-ultraviolet induced inflammation composition according to claim 1 or 2, characterized in that: The content of curcumin in the turmeric extract is ≥1%.

4. The anti-ultraviolet induced inflammation composition according to claim 1, characterized in that: The epigallocatechin gallate is derived from green tea extract.

5. The anti-ultraviolet induced inflammation composition according to any one of claims 1, 2 or 4, characterized in that: The purity of the epigallocatechin gallate is ≥94%.

6. The method for preparing the anti-ultraviolet induced inflammation composition according to any one of claims 1 to 5, characterized in that: include, The turmeric extract, epigallocatechin gallate and burdock extract are uniformly mixed to prepare the composition.

7. Use of the anti-ultraviolet induced inflammation composition according to any one of claims 1 to 5 in the preparation of medicines, health foods and foods for reducing the level of skin inflammatory factor TNF-α.

8. Use of the anti-ultraviolet induced inflammation composition according to any one of claims 1 to 5 in the preparation of drugs, health foods and foods for reducing the level of skin inflammatory factor IL-6.

9. Use of the anti-ultraviolet induced inflammation composition according to any one of claims 1 to 5 in the preparation of food, health food and medicine.

10. The use according to claim 9, characterized in that: The food and health food include drinks, jellies, solid drinks, candies and soft capsules, hard capsules, tablets and biscuits.

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