Culture equipment and culture method for strong rooting and disease prevention composite rhizosphere PGPR small strain

Abstract

The invention relates to the technical field of strain culture, and discloses culture equipment and a culture method for strong rooting and disease prevention composite rhizosphere PGPR small strains, the culture equipment comprises a culture device and a fixing frame, the culture device comprises an outer shell, an inner shell is arranged in the outer shell, a coiled pipe is arranged on the outer surface of the inner shell in a surrounding mode, and the coiled pipe is arranged in the outer shell. An auxiliary mechanism is arranged at the top of the culture device; the auxiliary mechanism comprises a vertical plate, and the vertical plate is fixedly installed on the top of the shell. According to the culture equipment and culture method for the strong rooting and disease prevention composite rhizosphere PGPR small strain, through the design of the auxiliary mechanism, the stirring device moves up and down and rotates in the culture process, the mixing uniformity of the strain and a culture solution is greatly enhanced, the consistency and high efficiency of the culture effect are ensured, meanwhile, the stirring device is provided with a cleaning brush, and the cleaning brush is convenient to clean. The inner shell can be automatically cleaned after culture is finished, residues are effectively removed, and the cleanliness of the equipment and the reliability of subsequent use are improved.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial strain cultivation, and in particular to a cultivation device and a cultivation method for a composite rhizosphere PGPR microbial strain with strong rooting and disease prevention properties. Background Art

[0002] Powerful rooting and disease prevention composite rhizosphere PGPR microbial species, this is a type of root-based bacteria that colonizes the rhizosphere of crops and is closely related to the roots of crops. When inoculated into crop seeds, roots, tubers, rhizomes or soil, it can promote plant growth and prevent diseases. It mainly includes Pseudomonas (such as Pseudomonas fluorescens, Pseudomonas syringae, etc.) and Bacillus, etc.

[0003] After searching, for example, the utility model patent with Chinese patent publication number CN221254581 U discloses a microbial fertilizer strain culture device. The microbial fertilizer strain culture device provided by the utility model can recycle the water injected into the tank body, protect and save water resources, and does not need to open the tank body to manually add strains and culture liquid, thereby avoiding the damage of the strain culture environment.

[0004] However, the equipment in the above utility model patent is inconvenient to clean during actual use. Since there may be residual bacteria, culture fluid and other impurities inside the tank, if these residues are not cleaned, it may have a negative impact on the subsequent bacteria culture process, and then affect the culture effect and product quality. Therefore, a culture equipment and method for a powerful rooting and disease prevention composite rhizosphere PGPR small bacteria strain is proposed. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the shortcomings of the prior art, the present invention provides a cultivation device and a cultivation method for a powerful rooting and disease-preventing composite rhizosphere PGPR small bacterial strain, which has the advantages of easy cleaning, etc., and solves the problem that impurities such as bacterial strains and culture fluid may remain in the tank, and if these residues are not cleaned, they may have a negative impact on the subsequent bacterial strain cultivation process.

[0007] (II) Technical solution

[0008] In order to achieve the above-mentioned purpose of easy cleaning, the present invention provides the following technical solutions: a culture device for a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria, comprising a culture device and a fixing frame, the culture device comprising an outer shell, an inner shell is arranged inside the outer shell, a serpentine tube is arranged around the outer surface of the inner shell, and an auxiliary mechanism is arranged on the top of the culture device;

[0009] The auxiliary mechanism includes a vertical plate, the top of the shell is fixedly installed with the vertical plate, the rear side of the vertical plate is fixedly installed with a motor 1, a turntable is fixedly installed at the output shaft of the motor 1, a movable shaft is movably installed on the front side of the turntable, a movable plate is movably installed on the end of the movable shaft away from the turntable, a guide column is fixedly installed on the top of the shell, the outer surfaces of the two guide columns are slidably installed with guide sleeves fixedly connected to one side of the movable plate, and a stirring device is arranged on the movable plate.

[0010] Preferably, the stirring device comprises a second motor, the second motor is fixedly mounted on the top of the movable plate, and a stirring rod extending into the inner shell is fixedly mounted on the output shaft of the second motor.

[0011] Preferably, a cross bar is fixedly mounted on the left side of the stirring rod, a cleaning brush is fixedly mounted on the bottom left end of the cross bar, and the cleaning brush is in contact with the inner wall of the inner shell.

[0012] Preferably, both ends of the movable shaft are respectively hinged between the turntable and the movable plate.

[0013] Preferably, a temperature sensor is provided on the top of the outer shell, and a detection probe of the temperature sensor extends to the inside of the inner shell.

[0014] Preferably, a strain addition tube and a culture solution addition tube are provided on the top of the shell, and a controller and a material extraction tube are provided on the front side of the shell.

[0015] Preferably, a water delivery device is provided on the top of the fixing frame, and the water delivery device includes a water tank. The water tank is provided on the top of the fixing frame, a heating plate is provided inside the water tank, a water pump is provided on the top of the water tank, and a water suction pipe is connected between the inlet of the water pump and the water tank.

[0016] Preferably, an annular tube is provided on the inner upper side of the inner shell, a cleaning tube is provided between the outlet of the water pump and the annular tube, and a nozzle is provided at the bottom of the annular tube.

[0017] Preferably, a water pipe is provided on the left side of the cleaning pipe, and one end of the water pipe is connected to the serpentine pipe, and valves are provided on both the cleaning pipe and the water pipe.

[0018] A method for cultivating a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria, comprising a method for cultivating a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria using a cultivation device, wherein the cultivation method is as follows:

[0019] 1) First, the controller sets the required temperature for cultivation, and starts the heating plate to heat the water in the water tank. The valve on the water pipe is opened, and the water pump is started to transport the heated water to the serpentine pipe through the water pipe to heat the inner shell. During this process, the temperature sensor monitors the temperature of the inner shell in real time to ensure that the cultivation temperature is appropriate.

[0020] 2) Add the strain from the strain adding tube to the inner shell, add the culture solution from the culture solution adding tube to the inner shell, and operate the auxiliary mechanism, start the motor 1, the motor 1 drives the turntable to rotate, the turntable drives the movable shaft to rotate, the movable shaft drives the movable plate to stably move up and down on the surfaces of the two guide columns through the guide sleeves on the left and right sides, the movable plate drives the stirring device to move up and down, and at the same time, the motor 2 in the stirring device drives the stirring rod to rotate. At this time, the stirring rod is in a state of moving up and down and rotating back and forth. The reciprocating up and down movement can increase the uniformity and depth of stirring, ensuring that the strain and the culture solution in each part of the inner shell can be fully stirred;

[0021] 3) When cleaning is required after the cultivation is finished, the valve on the water delivery pipe is closed and the valve on the cleaning pipe is opened, the heating plate is turned off, and the water source in the water storage tank is transported to the annular pipe through the water suction pipe, the water pump and the cleaning pipe and sprayed to the inner shell through the nozzle. At the same time, the auxiliary mechanism causes the stirring rod to continue to move up and down and rotate, and the stirring rod drives the cross bar and the cleaning brush to move up and down and rotate. In this process, the inner wall of the inner shell is cleaned by the cleaning brush and the water source at the same time;

[0022] 4) After cleaning is completed, the waste liquid is discharged from the material taking pipe.

[0023] Beneficial Effects

[0024] Compared with the prior art, the present invention provides a culture device and a culture method for a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria, which has the following beneficial effects:

[0025] The cultivation equipment and method of the powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacterial strain, through the design of auxiliary mechanisms, realize the up and down movement and rotation of the stirring device during the cultivation process, greatly enhancing the mixing uniformity of the strain and the culture solution, ensuring the consistency and efficiency of the cultivation effect; at the same time, the stirring device is equipped with a cleaning brush, which can automatically clean the inner shell after the cultivation is completed, effectively remove residues, and improve the cleanliness of the equipment and the reliability of subsequent use. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0027] Figure 2 It is a cross-sectional view of the present invention;

[0028] Figure 3 For the present invention Figure 1 Schematic diagram of the structure of the middle part;

[0029] Figure 4 It is a structural schematic diagram of the serpentine tube of the present invention;

[0030] Figure 5 It is a schematic diagram of the three-dimensional structure of the stirring device of the present invention;

[0031] Figure 6 It is a schematic diagram of the three-dimensional structure of the annular tube of the present invention.

[0032] In the figure: 1. culture device; 101. inner shell; 102. outer shell; 103. serpentine tube; 104. temperature sensor; 105. bacteria adding tube; 106. culture solution adding tube; 2. fixed frame; 3. auxiliary mechanism; 301. vertical plate; 302. motor 1; 303. turntable; 304. movable shaft; 305. moving plate; 306. guide column; 307. guide sleeve; 308. stirring device; 401. motor 2; 402. stirring rod; 403. cross bar; 404. cleaning brush; 5. water delivery device; 501. water storage tank; 502. water pump; 503. cleaning pipe; 504. ring pipe; 505. nozzle; 506. water delivery pipe. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] See also Figure 1-6 A cultivation device for a powerful rooting and disease-preventing composite rhizosphere PGPR microbial species includes a cultivation device 1 and a fixing frame 2. The cultivation device 1 includes an outer shell 102, an inner shell 101 is arranged inside the outer shell 102, a serpentine tube 103 is arranged around the outer surface of the inner shell 101, and an auxiliary mechanism 3 is arranged on the top of the cultivation device 1.

[0035] Embodiment 1:

[0036] The auxiliary mechanism 3 includes a vertical plate 301, the vertical plate 301 is fixedly installed on the top of the shell 102, a motor 302 is fixedly installed on the rear side of the vertical plate 301, a turntable 303 is fixedly installed at the output shaft of the motor 302, a movable shaft 304 is movably installed on the front side of the turntable 303, a movable plate 305 is movably installed on the end of the movable shaft 304 away from the turntable 303, a guide column 306 is fixedly installed on the top of the shell 102, and guide sleeves 307 fixedly connected to one side of the movable plate 305 are slidably installed on the outer surfaces of the two guide columns 306, and a stirring device 308 is arranged on the movable plate 305.

[0037] like Figure 2 and Figure 5 As shown, the stirring device 308 includes a motor 2 401 , a stirring rod 402 , a cross bar 403 and a cleaning brush 404 . When the motor 2 401 is started, the stirring rod 402 and the cleaning brush 404 can be driven to rotate. The stirring rod 402 evenly mixes the bacteria and the mixed liquid, and the cleaning brush 404 cleans the inner wall of the inner shell 101 .

[0038] like Figure 2 and Figure 3 As shown, the auxiliary mechanism 3 can drive the movable plate 305 to move up and down through the operation of the motor 302, the rotation of the turntable 303 and the movable shaft 304, and the sliding cooperation between the guide column 306 and the guide sleeve 307, thereby driving the stirring device 308 to move up and down, thereby increasing the uniformity and depth of stirring, and ensuring that the strains and culture fluid can be fully stirred in each part of the inner shell 101.

[0039] like Figure 2 and Figure 4 As shown, the water delivery device 5 includes a water storage tank 501, a water pump 502, a water suction pipe, a cleaning pipe 503, a ring pipe 504 and a water delivery pipe 506. By arranging the water pump 502, the water suction pipe and the serpentine pipe 103, water can be transported to the serpentine pipe 103 to heat the inner shell 101 and provide temperature for the cultivation of bacteria. By arranging the temperature sensor 104, it is convenient to monitor the temperature of the inner shell 101 and control the opening and closing of the heating plate to ensure that the cultivation temperature is appropriate.

[0040] like Figure 2 and Figure 6 As shown, by providing a water pump 502, a water suction pipe, a cleaning pipe 503 and a ring pipe 504, water can be transported into the cleaning pipe 503 and sprayed out through the nozzle 505 for easy cleaning.

[0041] Embodiment 2:

[0042] A method for cultivating a powerful rooting and disease prevention composite rhizosphere PGPR micro-bacteria, including a method for cultivating a powerful rooting and disease prevention composite rhizosphere PGPR micro-bacteria using a cultivation device, and the cultivation method is as follows:

[0043] 1) First, the controller sets the required temperature for cultivation, and starts the heating plate to heat the water in the water tank 501, opens the valve on the water pipe 506, and starts the water pump 502 to transport the heated water to the serpentine tube 103 through the water pipe 506 to heat the inner shell 101. During this process, the temperature sensor 104 monitors the temperature of the inner shell 101 in real time to ensure that the cultivation temperature is appropriate;

[0044] 2) Add the bacterial strain from the bacterial strain adding tube 105 to the inner shell 101, add the culture solution from the culture solution adding tube 106 to the inner shell 101, and operate the auxiliary mechanism 3, start the motor 1 302, the motor 1 302 drives the turntable 303 to rotate, the turntable 303 drives the movable shaft 304 to rotate, the movable shaft 304 drives the movable plate 305 to move up and down stably on the surfaces of the two guide pillars 306 through the guide sleeves 307 on the left and right sides, the movable plate 305 drives the stirring device 308 to move up and down, and at the same time, the motor 2 401 in the stirring device 308 drives the stirring rod 402 to rotate, at this time, the stirring rod 402 is in a state of moving up and down and rotating back and forth, and the reciprocating up and down movement can increase the uniformity and depth of stirring, ensuring that the bacterial strain and the culture solution in each part of the inner shell 101 can be fully stirred;

[0045] 3) When cleaning is required after the cultivation is finished, the valve on the water delivery pipe 506 is closed and the valve on the cleaning pipe 503 is opened, the heating plate is turned off, and the water source in the water storage tank 501 is transported to the annular pipe 504 through the water suction pipe, the water pump 502 and the cleaning pipe 503 and sprayed to the inner shell 101 through the nozzle 505. At the same time, the auxiliary mechanism 3 causes the stirring rod 402 to continue to move up and down and rotate, and the stirring rod 402 drives the cross bar 403 and the cleaning brush 404 to move up and down and rotate. In this process, the inner wall of the inner shell 101 is cleaned by the cleaning brush 404 and the water source at the same time;

[0046] 4) After cleaning is completed, the waste liquid is discharged from the material taking pipe.

[0047] In summary, the cultivation equipment and method of the powerful rooting and disease-preventing composite rhizosphere PGPR microbial species, through the design of the auxiliary mechanism 3, realizes the up and down movement and rotation of the stirring device 308 during the cultivation process, greatly enhances the mixing uniformity of the strain and the culture solution, and ensures the consistency and efficiency of the cultivation effect. At the same time, the stirring device 308 is equipped with a cleaning brush 404, which can automatically clean the inner shell 101 after the cultivation is completed, effectively remove residues, and improve the cleanliness of the equipment and the reliability of subsequent use.

[0048] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0049] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cultivation device for a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria, comprising a cultivation device (1) and a fixing frame (2), characterized in that: The culture device (1) comprises an outer shell (102), an inner shell (101) is arranged inside the outer shell (102), a serpentine tube (103) is arranged around the outer surface of the inner shell (101), and an auxiliary mechanism (3) is arranged on the top of the culture device (1); The auxiliary mechanism (3) comprises a vertical plate (301), the vertical plate (301) is fixedly mounted on the top of the housing (102), a motor (302) is fixedly mounted on the rear side of the vertical plate (301), a turntable (303) is fixedly mounted on the output shaft of the motor (302), a movable shaft (304) is movably mounted on the front side of the turntable (303), a movable plate (305) is movably mounted on one end of the movable shaft (304) away from the turntable (303), a guide column (306) is fixedly mounted on the top of the housing (102), guide sleeves (307) fixedly connected to one side of the movable plate (305) are slidably mounted on the outer surfaces of the two guide columns (306), and a stirring device (308) is arranged on the movable plate (305).

2. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 1, characterized in that: The stirring device (308) comprises a second motor (401), the second motor (401) is fixedly mounted on the top of the movable plate (305), and a stirring rod (402) extending into the interior of the inner shell (101) is fixedly mounted on the output shaft of the second motor (401).

3. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 2 is characterized in that: A cross bar (403) is fixedly mounted on the left side of the stirring rod (402), a cleaning brush (404) is fixedly mounted on the bottom left end of the cross bar (403), and the cleaning brush (404) is in contact with the inner wall of the inner shell (101).

4. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 1, characterized in that: The two ends of the movable shaft (304) are respectively hinged between the rotating disk (303) and the movable plate (305).

5. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 1, characterized in that: A temperature sensor (104) is arranged on the top of the outer shell (102), and a detection probe of the temperature sensor (104) extends into the interior of the inner shell (101).

6. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 1, characterized in that: The top of the shell (102) is provided with a strain addition tube (105) and a culture solution addition tube (106), and the front side of the shell (102) is provided with a controller and a material extraction tube.

7. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 1, characterized in that: A water delivery device (5) is arranged on the top of the fixing frame (2), and the water delivery device (5) comprises a water storage tank (501). The water storage tank (501) is arranged on the top of the fixing frame (2), a heating plate is arranged inside the water storage tank (501), a water pump (502) is arranged on the top of the water storage tank (501), and a water suction pipe is connected between the inlet of the water pump (502) and the water storage tank (501).

8. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 7, characterized in that: An annular tube (504) is provided on the inner upper side of the inner shell (101), a cleaning tube (503) is provided between the outlet of the water pump (502) and the annular tube (504), and a nozzle (505) is provided at the bottom of the annular tube (504).

9. The cultivation equipment of a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria according to claim 8, characterized in that: A water pipe (506) is arranged on the left side of the cleaning pipe (503), and one end of the water pipe (506) is connected to the serpentine pipe (103). Valves are arranged on both the cleaning pipe (503) and the water pipe (506).

10. A method for cultivating a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria, comprising using the cultivation equipment according to any one of claims 1 to 9 to cultivate a powerful rooting and disease-preventing composite rhizosphere PGPR micro-bacteria, characterized in that: The culture method is as follows: 1) First, the controller sets the required temperature for cultivation, and starts the heating plate to heat the water source in the water tank (501), opens the valve on the water pipe (506), and starts the water pump (502), and transports the heated water source to the serpentine tube (103) through the water pipe (506) to heat the inner shell (101). During this process, the temperature sensor (104) monitors the temperature of the inner shell (101) in real time to ensure that the cultivation temperature is appropriate; 2) adding bacterial strains from the bacterial strain adding tube (105) to the inner shell (101), adding culture fluid from the culture fluid adding tube (106) to the inner shell (101), and operating the auxiliary mechanism (3), starting the motor 1 (302), the motor 1 (302) drives the turntable (303) to rotate, the turntable (303) drives the movable shaft (304) to rotate, the movable shaft (304) drives the movable plate (305) to stably move up and down on the surfaces of the two guide pillars (306) through the guide sleeves (307) on the left and right sides, the movable plate (305) drives the stirring device (308) to move up and down, and at the same time, the motor 2 (401) in the stirring device (308) drives the stirring rod (402) to rotate, at which time the stirring rod (402) is in a state of moving up and down and rotating back and forth, and the reciprocating up and down movement can increase the uniformity and depth of stirring, ensuring that the bacterial strains and the culture fluid can be fully stirred in each part of the inner shell (101); 3) When the culture is finished and cleaning is required, the valve on the water delivery pipe (506) is closed and the valve on the cleaning pipe (503) is opened, the heating plate is turned off, and the water source in the water storage tank (501) is transported to the annular pipe (504) through the water suction pipe, the water pump (502) and the cleaning pipe (503) and sprayed to the inner shell (101) through the nozzle (505). At the same time, the auxiliary mechanism (3) causes the stirring rod (402) to continue to move up and down and rotate, and the stirring rod (402) drives the cross bar (403) and the cleaning brush (404) to move up and down and rotate. In this process, the inner wall of the inner shell (101) is cleaned by the cleaning brush (404) and the water source at the same time; 4) After cleaning is completed, the waste liquid is discharged from the material taking pipe.

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