Slice collecting assembly and slice collecting system

Abstract

The invention relates to the technical field of slice collection, and provides a slice collection assembly and a slice collection system.The slice collection assembly comprises a first driving device, a collection tank, a collection cover plate and a porous plate, the first driving device is connected with the collection tank and drives the collection tank to rotate relative to the collection cover plate, and the collection tank is provided with an upward opening; a liquid outlet used for being connected with the pipeline assembly is formed in the collecting tank; the collecting cover plate covers the opening in a sealing mode, and a liquid inlet used for being connected with the pipeline assembly is formed in the collecting cover plate; the perforated plate is detachably mounted in the collecting tank and can rotate along with the collecting tank, a plurality of collecting holes are formed in the perforated plate at intervals in the rotating direction of the perforated plate, and the collecting holes are hollowed out; and in the process that the porous plate rotates along with the collecting tank, the collecting holes can be aligned with the liquid inlets respectively. According to the section collecting assembly, in the section collecting process, the opening of the collecting groove is always sealed by the collecting cover plate, so that the section is slightly influenced by external factors, and later culture of the section is facilitated.

Description

Technical Field

[0001] The present application relates to the field of slice collection technology, and in particular to a slice collection component and a slice collection system. Background Art

[0002] In the study of clinical anticancer drug screening for personalized treatment, the slicing and culturing of tumor tissues are key steps. The usual practice is to slice in a liquid environment, the generated slices float in the liquid, and the slices are manually picked out and placed in collection containers such as multi-well plates, which is time-consuming and labor-intensive.

[0003] At present, there are automatic slice collection systems on the market, including a slice tank, a vibrating slicer, a pipeline assembly, a porous plate and a collection tank. The slice tank is filled with a buffer solution, and the tool of the vibrating slicer is arranged in the slice tank to cut the sample in the slice tank into slices; the porous plate is arranged in the collection tank, and the pipeline assembly is used to send the slices cut by the vibrating slicer into the collection holes of the porous plate. However, the porous plate in the above-mentioned collection tank is in an exposed state. During the process of collecting slices by the porous plate, the slices are greatly affected by external factors (such as temperature and air cleanliness), which in turn affects the later culture of the slices. Summary of the invention

[0004] In view of this, the embodiments of the present application provide a slice collection component and a slice collection system to solve the problem that the slices are greatly affected by external factors.

[0005] The first aspect of the present application proposes a slice collection assembly, including a first driving device, a collection trough, a collection cover and a porous plate, wherein the first driving device is connected to the collection trough and drives the collection trough to rotate relative to the collection cover, the collection trough has an upward opening, and the collection trough is provided with a liquid outlet for connecting to a pipeline assembly; the collection cover covers the opening, and the collection cover is provided with a liquid inlet for connecting to the pipeline assembly; the porous plate is detachably installed in the collection trough and can rotate with the collection trough, and the porous plate is provided with a plurality of collection holes arranged at intervals along its rotation direction, and the bottom wall and / or the side wall of the collection hole are provided with hollowing; during the rotation of the porous plate with the collection trough, the plurality of collection holes can be aligned with the liquid inlet respectively.

[0006] The beneficial effects of the slice collection assembly provided in the embodiment of the present application are as follows: when the slice collection assembly collects slices, the porous plate is in the collection tank, the collection cover plate covers the opening of the collection tank, and the pipeline assembly uses liquid to send the slices into the collection holes of the porous plate through the liquid inlet. Since the bottom wall and / or the side wall of the collection hole are hollowed out, the liquid can enter the collection tank and flow out from the liquid outlet of the collection tank, while the slices remain in the collection holes. Moreover, it is only necessary to drive the porous plate to rotate through the first driving device so that the multiple collection holes of the porous plate are respectively aligned with the liquid inlet, so that multiple slices can be collected, and the collection efficiency is relatively high. Since the collection cover plate always covers the opening of the collection tank during the collection process, the slices are less affected by external factors, which is beneficial to the later culture of the slices.

[0007] In some embodiments, a first annular recess is provided on the top of the collecting trough, and a first annular flange is provided on the edge of the collecting cover plate extending into the first annular recess.

[0008] The beneficial effect of adopting the above technical solution is that after the first annular flange extends into the first annular recess, a U-shaped channel can be formed, making the slice less susceptible to external influences.

[0009] In some embodiments, a sealing liquid is disposed in the first annular recess.

[0010] The beneficial effects of adopting the above technical solution are as follows: the sealing liquid can play a sealing role to prevent the slices from being affected by the outside world during the collection process; moreover, the sealing liquid can also play a lubricating role, which is conducive to the rotation of the collection trough relative to the collection cover.

[0011] In some embodiments, the bottom of the collecting hole is provided with a plurality of supporting protrusions for supporting the slices.

[0012] The beneficial effect of adopting the above technical solution is that during culture, both the upper and lower sides of the slice can be infiltrated by the culture solution, ensuring the long-term activity of the slice.

[0013] In some embodiments, an anti-rotation recess is provided at the top of the collecting trough, and an anti-rotation protrusion is provided on the porous plate to cooperate with the anti-rotation recess; or, an anti-rotation protrusion is provided at the top of the collecting trough, and an anti-rotation recess is provided on the porous plate to cooperate with the anti-rotation protrusion.

[0014] The beneficial effect of adopting the above technical solution is that the anti-rotation concave portion cooperates with the anti-rotation convex portion to facilitate taking and placing the porous plate in the collecting tank while ensuring that the porous plate and the collecting tank are relatively fixed in the rotation direction.

[0015] In some embodiments, the slice collection assembly also includes a first bracket, and the first driving device drives the collection trough to rotate through the first bracket; and / or, the slice collection assembly also includes a second driving device and a second bracket, and the second driving device drives the collection cover plate to rise and fall through the second bracket.

[0016] The beneficial effects of adopting the above technical solution are as follows: the first driving device drives the collection tank to rotate through the first bracket, and the liquid outlet can be set at the bottom of the collection tank, which is beneficial to the liquid discharge; the second driving device drives the collection cover plate to rise and fall through the second bracket, which is beneficial to the taking and placing of the porous plate in the collection tank and reduces labor intensity.

[0017] In some embodiments, the first driving device includes a first driving motor and a reducer connected to the first driving motor, and the reducer has a through hole for a corresponding pipeline of the pipeline assembly to pass through.

[0018] The beneficial effect of adopting the above technical solution is that the corresponding pipeline of the pipeline assembly is connected to the liquid outlet and then passes through the perforation to avoid entanglement of the pipeline, which is beneficial to the flow of liquid in the pipeline.

[0019] The second aspect of the present application proposes a slice collection system, which includes a slice trough, a slicer, a pipeline assembly and the slice collection assembly as described in the first aspect, wherein the cutter of the slicer is located in the slice trough, and the pipeline assembly is connected between the slice trough, the collection trough and the collection cover plate to deliver the slices cut by the slicer into the collection holes of the porous plate.

[0020] The slice collection system adopts any one or more embodiments of the above-mentioned slice collection components, and thus has the beneficial effects of the above-mentioned embodiments, which will not be described one by one here.

[0021] In some embodiments, the pipeline assembly includes a four-way pipe, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline, a fifth pipeline, a sixth pipeline, a first drive pump and a second drive pump. The four-way pipe includes a main body and a first interface, a second interface, a third interface and a fourth interface arranged on the main body; the two ends of the first pipeline are respectively connected to the slicing groove and the first interface, the two ends of the second pipeline are respectively connected to the second interface and the inlet of the first drive pump, and the two ends of the third pipeline are respectively connected to the outlet of the first drive pump and the slicing groove; the fourth pipeline is respectively connected to the liquid outlet and the inlet of the second drive pump, the fifth pipeline is respectively connected to the outlet of the second drive pump and the third interface, and the sixth pipeline is respectively connected to the fourth interface and the liquid inlet; a filter is provided at the second interface, and valves are provided on the first pipeline, the second pipeline, the fifth pipeline and the sixth pipeline; wherein, the first drive pump and the second drive pump are independently or integratedly arranged.

[0022] In some embodiments, at least one of the first interface, the second interface, the third interface, and the fourth interface is detachably connected to the body.

[0023] The beneficial effects of adopting the above technical solution are: it is easy to disassemble and assemble the filter screen, which is beneficial to the replacement of the filter screen.

[0024] In some embodiments, the pipeline assembly includes a seventh pipeline, an eighth pipeline, a ninth pipeline, a tenth pipeline, a third drive pump and a fourth drive pump, the two ends of the seventh pipeline are respectively connected to the slicing groove and the inlet of the third drive pump, the two ends of the eighth pipeline are respectively connected to the outlet of the third drive pump and the liquid inlet, the two ends of the ninth pipeline are respectively connected to the liquid outlet and the inlet of the fourth drive pump, and the two ends of the tenth pipeline are respectively connected to the outlet of the fourth drive pump and the slicing groove; wherein the third drive pump and the fourth drive pump are independently or integratedly arranged.

[0025] In some embodiments, at least one pipeline of the pipeline assembly is provided with a cooler for cooling the liquid.

[0026] The beneficial effects of adopting the above technical solution are: ensuring that the liquid is always at the set temperature during the slice collection process, avoiding the impact of excessive temperature on the slices, and ensuring the activity and integrity of the slices in subsequent experiments.

[0027] In some embodiments, the pipeline assembly includes a detection pipeline connected to the liquid inlet, and the slice collection system also includes a sensor corresponding to the detection pipeline, and the sensor is used to detect whether the slice passes through the detection pipeline.

[0028] The beneficial effect of adopting the above technical solution is: when the sensor detects that a slice has passed through the detection pipeline, it transmits a signal to collect the next slice, ensuring that there is a slice in each collection hole.

[0029] In some embodiments, a pusher is provided at the bottom of the slice groove for pushing the sample into the slice groove.

[0030] The beneficial effect of adopting the above technical solution is as follows: since the sample is in the pusher and is gradually pushed into the slicing groove, the sample hardly occupies the volume of the slicing groove, which is beneficial to reducing the size of the slicing groove and also avoids the sample being exposed to the outside for a long time.

[0031] In some embodiments, the slice collection system also includes a culture device, which includes a culture tray and a culture cover for covering the culture tray, the culture tray has a plurality of culture holes arranged at intervals along its circumference, and the plurality of culture holes are respectively arranged corresponding to the plurality of collection holes.

[0032] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or conventional technical descriptions will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0034] Figure 1 is a schematic diagram of the structure of a slice collection component provided in some embodiments of the present application;

[0035] Figure 2 yes Figure 1 A schematic diagram of the structure of the middle collecting tank, the collecting cover plate and the porous plate;

[0036] Figure 3 yes Figure 2 A schematic structural diagram of the collecting tank, the collecting cover plate and the porous plate from another perspective;

[0037] Figure 4 yes Figure 2 Schematic diagram of the structure of the medium porous plate;

[0038] Figure 5 yes Figure 4 The enlarged view of point A in the middle;

[0039] Figure 6 yes Figure 4 A schematic diagram of the structure of the medium porous plate from another perspective;

[0040] Figure 7 is a schematic diagram of the structure of a slice collection system provided in some embodiments of the present application;

[0041] Figure 8 yes Figure 7 A schematic diagram of the structure of the slice collection system from another perspective;

[0042] Fig. 9 yes Figure 8 Schematic diagram of the structure of the middle four-way;

[0043] Fig.10 is a schematic diagram of the structure of a slice collection system provided in some other embodiments of the present application;

[0044] Fig.11 yes Fig.10 A schematic diagram of the structure of the slice collection system from another perspective;

[0045] Fig.12 is a schematic diagram of the structure of a culture device provided in some embodiments of the present application;

[0046] Fig.13 It is a structural schematic diagram of a slice collection system provided in some further embodiments of the present application.

[0047] The meanings of the marks in the figure are:

[0048] 100. Slice collection system;

[0049] 10. Slice collection assembly; 11. First drive device; 111. Speed ​​reducer; 1111. Perforation; 112. First drive motor; 12. Collection tank; 121. Opening; 122. Liquid outlet; 123. First annular recess; 124. Anti-rotation recess; 13. Collection cover; 131. Liquid inlet; 132. First annular flange; 133. First connecting arm; 14. Perforated plate; 141. Collection hole; 1411. Hollowing; 1412. Support convex portion; 142. Anti-rotation convex portion; 15. First bracket; 16. Second drive device; 161. Second drive motor; 162. Screw nut mechanism; 17. Second bracket;

[0050] 20. Slicing slot;

[0051] 30. Knives;

[0052] 41, cross-way; 411, body; 412, first interface; 413, second interface; 4131, filter screen installation part; 414, third interface; 415, fourth interface; 42, first pipeline; 421, first valve; 43, second pipeline; 431, second valve; 44, third pipeline; 45, fourth pipeline; 46, fifth pipeline; 461, third valve; 47, sixth pipeline; 471, fourth valve; 48, first drive pump; 49, second drive pump;

[0053] 51, seventh pipeline; 52, eighth pipeline; 53, ninth pipeline; 54, tenth pipeline; 55, third drive pump; 56, fourth drive pump;

[0054] 60. Cooler;

[0055] 70. Sensors;

[0056] 80. Thruster;

[0057] 90. Culture device; 91. Culture tray; 911. Culture hole; 912. Second annular recess; 913. First center hole; 92. Culture cover; 921. Second annular flange; 922. Second center hole. DETAILED DESCRIPTION

[0058] The following embodiments of the technical solution of the present application are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0059] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" in the specification and claims of this application and the above-mentioned figure descriptions and any variations thereof are intended to cover non-exclusive inclusions.

[0060] In the description of the embodiments of the present application, the technical terms "first", "second", etc. are only used to distinguish different objects, and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the meaning of "multiple" is more than two, unless otherwise clearly and specifically defined.

[0061] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0062] In the description of the embodiments of the present application, the term "and / or" is only a description of the association relationship of the associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0063] In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two). Similarly, "multiple groups" refers to more than two groups (including two groups), and "multiple pieces" refers to more than two pieces (including two pieces).

[0064] In the description of the embodiments of the present application, the orientations or positional relationships indicated by technical terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "vertical", "horizontal", "top", "bottom", "inside" and "outside" are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the embodiments of the present application.

[0065] In the description of the embodiments of the present application, unless otherwise clearly specified and limited, technical terms such as "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0066] The first embodiment of the present application provides a slice collection component. Figure 1 , Figure 2 , Figure 3 and Figure 5The slice collection assembly 10 includes a first driving device 11, a collection tank 12, a collection cover plate 13 and a porous plate 14. The first driving device 11 is connected to the collection tank 12 and drives the collection tank 12 to rotate relative to the collection cover plate 13. The collection tank 12 has an upward opening 121, and the collection tank 12 is provided with a liquid outlet 122 for connecting to the pipeline assembly; the collection cover plate 13 covers the opening 121, and the collection cover plate 13 is provided with a liquid inlet 131 for connecting to the pipeline assembly; the porous plate 14 is detachably installed in the collection tank 12 and can rotate with the collection tank 12. The porous plate 14 is provided with a plurality of collection holes 141 arranged at intervals along the rotation direction thereof, and the bottom wall and / or the side wall of the collection hole 141 are provided with a hollow 1411; when the porous plate 14 rotates with the collection tank 12, the plurality of collection holes 141 can be aligned with the liquid inlet 131 respectively.

[0067] It is understandable that the first driving device 11 can be directly connected to the collecting tank 12 or can be indirectly connected. When the first driving device 11 is indirectly connected to the collecting tank 12, for example, the first driving device 11 can be connected to the collecting tank 12 via the first bracket 15.

[0068] The first driving device 11 is not limited in the present application, as long as it can drive the collection tank 12 to rotate relative to the collection cover plate 13. For example, the first driving device 11 may include a first driving motor 112 and a reducer 111 connected to the first driving motor 112, and the reducer 111 is directly or indirectly connected to the collection tank 12. Optionally, the first driving motor 112 is a stepping motor.

[0069] Optionally, the first driving device 11 drives the collecting tank 12 to rotate 360°, and then rotates 360° in the opposite direction, and repeats the cycle to avoid entanglement of the pipeline.

[0070] The collecting cover 13 covers the opening 121 of the collecting tank 12. It can be understood that a small gap can be reserved between the collecting cover 13 and the collecting tank 12 to avoid contact between the two, which is conducive to the rotation of the collecting tank 12 relative to the collecting cover 13; or, the collecting cover 13 and the collecting tank 12 can also be in contact. At this time, a self-lubricating coating (such as Teflon) can be provided on the contact surface of at least one of the collecting cover 13 and the collecting tank 12, which is conducive to the rotation of the collecting tank 12 relative to the collecting cover 13. Of course, the self-lubricating coating may not be provided.

[0071] It is understandable that the liquid outlet 122 can be disposed at the bottom of the collecting tank 12 or at the side of the collecting tank 12. When the liquid outlet 122 is disposed at the side of the collecting tank 12, the liquid outlet 122 can be disposed close to the bottom of the collecting tank 12 to facilitate liquid outflow.

[0072] It can be understood that the liquid inlet 131 can be arranged at the middle position of the collecting cover plate 13 , or at the edge position of the collecting cover plate 13 .

[0073] The porous plate 14 is detachably installed in the collecting tank 12 and can rotate with the collecting tank 12, which means that the porous plate 14 is relatively fixed with the collecting tank 12 in the rotation direction after being installed in the collecting tank 12. When the first driving device 11 drives the collecting tank 12 to rotate relative to the collecting cover plate 13, the porous plate 14 can rotate with the collecting tank 12 so that the multiple collecting holes 141 of the porous plate 14 are respectively aligned with the liquid inlet 131 of the collecting cover plate 13 to allow the slices to enter each collecting hole 141; when the slice collection is completed, the porous plate 14 can be removed from the collecting tank 12 for subsequent culture.

[0074] Optionally, the collecting tank 12 , the collecting cover plate 13 and the porous plate 14 are all circular structures; the multiple collecting holes 141 are arranged at intervals along the rotation direction of the porous plate 14 , that is, the multiple collecting holes 141 are arranged at intervals along the circumference of the porous plate 14 .

[0075] The plurality of collecting holes 141 can be evenly spaced in the rotation direction of the porous plate 14, so that the collecting tank 12 rotates at the same angle each time, which is beneficial to the driving design of the first driving device 11. Of course, the spacing between the plurality of collecting holes 141 can also be arranged at random, for example, the spacing between two collecting holes 141 gradually increases, or the spacing between two collecting holes 141 gradually decreases, or the spacing between two collecting holes 141 can first increase and then decrease, or the spacing between two collecting holes 141 can first decrease and then increase.

[0076] Hollows 1411 are provided on the bottom wall and / or the side wall of the collecting hole 141. It can be understood that hollows 1411 are provided on both the bottom wall and the side wall of the collecting hole 141, or hollows 1411 are provided only on the bottom wall of the collecting hole 141, or hollows 1411 are provided only on the side wall of the collecting hole 141. When hollows 1411 are provided on both the bottom wall and the side wall of the collecting hole 141, the hollows 1411 on the bottom wall and the hollows 1411 on the side wall may be connected or not connected.

[0077] The beneficial effects of the slice collection assembly 10 provided in the embodiment of the present application are as follows: when the slice collection assembly 10 collects slices, the porous plate 14 is in the collection tank 12, the collection cover plate 13 covers the opening 121 of the collection tank 12, and the pipeline assembly uses liquid to send the slices into the collection holes 141 of the porous plate 14 through the liquid inlet 131. Since the bottom wall and / or the side wall of the collection hole 141 are provided with hollows 1411, the liquid can enter the collection tank 12 and flow out from the liquid outlet 122 of the collection tank 12, while the slices stay in the collection holes 141. Moreover, it is only necessary to drive the porous plate 14 to rotate through the first driving device 11 so that the multiple collection holes 141 of the porous plate 14 are aligned with the liquid inlet 131 respectively, so that multiple slices can be collected, and the collection efficiency is relatively high. Since the collection cover plate 13 always covers the opening 121 of the collection tank 12 during the collection process, the slices are less affected by external factors, which is conducive to the later culture of the slices.

[0078] Among them, the liquid used for transporting slices can be a buffer solution, which can maintain pH value, preserve cell morphology, provide a stable ionic environment, etc.

[0079] Please also refer to Figure 2 and Figure 3 In some embodiments, a first annular recess 123 is provided at the top of the collecting tank 12 , and a first annular flange 132 extending into the first annular recess 123 is provided at the edge of the collecting cover plate 13 .

[0080] The first annular recess 123 and the first annular flange 132 are both circular ring structures.

[0081] Optionally, the radial width of the first annular recess 123 is greater than the thickness of the first annular flange 132 , so as to facilitate the insertion of the first annular flange 132 into the first annular recess 123 .

[0082] Based on the above solution, after the first annular flange 132 extends into the first annular recess 123, a U-shaped channel can be formed, so that the slices are less susceptible to external influences.

[0083] In other embodiments, the first annular recess 123 can be set on the collecting cover plate 13, and the top of the collecting tank 12 is inserted into the first annular recess 123; or, the first annular recess 123 is not set on the top of the collecting tank 12, and the first annular flange 132 is not set on the collecting cover plate 13, that is, the collecting cover plate 13 is a flat plate structure.

[0084] In some embodiments, a sealing liquid is disposed in the first annular recess 123 .

[0085] Optionally, the sealing liquid may be water, buffer solution, etc., so as to prevent the sealing liquid from entering the collecting tank 12 and contaminating the liquid for transporting the slices.

[0086] Based on the above solution, the sealing liquid can play a sealing role to prevent the slices from being affected by the outside world during the collection process; moreover, the sealing liquid can also play a lubricating role, which is conducive to the rotation of the collection tank 12 relative to the collection cover plate 13.

[0087] In other embodiments, the sealing liquid may not be provided in the first annular recess 123 .

[0088] Please also refer to Figure 4 and Figure 5 In some embodiments, a plurality of supporting protrusions 1412 for supporting slices are provided at the bottom of the collecting hole 141 .

[0089] Optionally, the support protrusion 1412 is a support convex point to reduce the contact area with the slice, so that the lower side of the slice can contact the culture solution more during culture. Of course, the support protrusion 1412 can also be a support bar, wherein the support bar can be a straight bar extending along the radial direction of the collection hole 141, or an arc bar or an annular bar extending along the circumference of the collection hole 141.

[0090] Based on the above scheme, both the upper and lower sides of the slice can be infiltrated by the culture solution during culture, ensuring the long-term activity of the slice.

[0091] Please also refer to Figure 2 , Figure 3 and Figure 6 In some embodiments, an anti-rotation recess 124 is provided on the top of the collecting tank 12 , and an anti-rotation protrusion 142 cooperating with the anti-rotation recess 124 is provided on the porous plate 14 .

[0092] Optionally, in the radial direction of the collecting tank 12, the anti-rotation recess 124 is located inside the first annular recess 123. Among them, one, two, or three or more anti-rotation recesses 124 can be provided; when two anti-rotation recesses 124 are provided, the two anti-rotation recesses 124 are symmetrically arranged around the center of the collecting tank 12.

[0093] Optionally, the anti-rotation protrusion 142 is arranged in one-to-one correspondence with the anti-rotation recess 124. For example, when one anti-rotation recess 124 is provided, one anti-rotation protrusion 142 is provided; when multiple anti-rotation recesses 124 are provided, multiple anti-rotation protrusions 142 are provided.

[0094] The anti-rotation recess 124 is matched with the anti-rotation protrusion 142 . For example, the cross-sections of the two are rectangular, semicircular, triangular, etc.

[0095] Based on the above solution, the anti-rotation recess 124 cooperates with the anti-rotation protrusion 142 to facilitate the placement and removal of the porous plate 14 in the collecting tank 12 while ensuring that the porous plate 14 and the collecting tank 12 are relatively fixed in the rotation direction.

[0096] In other embodiments, an anti-rotation protrusion 142 is provided on the top of the collecting tank 12 , and an anti-rotation concave portion 124 cooperating with the anti-rotation protrusion 142 is provided on the porous plate 14 .

[0097] In other embodiments, the collecting tank 12 is provided with a threaded hole, the porous plate 14 is provided with a through hole, and the porous plate 14 is detachably mounted on the collecting tank 12 by means of threaded fasteners (such as screws, bolts, etc.) passing through the through hole and screwed into the threaded hole.

[0098] Please refer to Figure 1 In some embodiments, the slice collecting assembly 10 further includes a second driving device 16 and a second bracket 17 , and the second driving device 16 drives the collecting cover plate 13 to rise and fall through the second bracket 17 .

[0099] The second drive device 16 is not limited in the present application, as long as it can drive the collection cover plate 13 to rise and fall. For example, the second drive device 16 may include a second drive motor 161 and a lead screw nut mechanism 162 connected to the second drive motor 161, the lead screw nut mechanism 162 includes a vertically arranged lead screw and a slider connected to the lead screw, the lead screw is connected to the drive shaft of the second drive motor 161, the slider is connected to the second bracket 17, and the second drive motor 161 drives the lead screw to rotate when working, and the lead screw drives the slider and the second bracket 17 to move vertically, thereby driving the collection cover plate 13 to rise and fall. Of course, the second drive device 16 can also be a vertically arranged telescopic cylinder, the telescopic cylinder of the telescopic cylinder drives the second bracket 17 to move vertically, thereby driving the collection cover plate 13 to rise and fall. Optionally, the second drive motor 161 is a stepping motor.

[0100] Optionally, a first connecting arm 133 is provided on the collecting cover plate 13 , and the collecting cover plate 13 is connected to the second bracket 17 via the first connecting arm 133 .

[0101] Based on the above solution, the second driving device 16 drives the collecting cover plate 13 to rise and fall through the second bracket 17, which is beneficial to taking and placing the porous plate 14 in the collecting tank 12 and reducing labor intensity.

[0102] In other embodiments, the second driving device 16 may not be provided, and the collecting cover plate 13 may be manually raised or lowered. Alternatively, only the second driving device 16 may be provided without the second bracket 17, and the second driving device 16 may directly drive the collecting cover plate 13 to be raised or lowered.

[0103] Please refer to Figure 1 In some embodiments, the first driving device 11 includes a first driving motor 112 and a reducer 111 connected to the first driving motor 112, and the reducer 111 has a through hole 1111 for the corresponding pipeline of the pipeline assembly to pass through.

[0104] Optionally, the liquid outlet 122 is disposed away from the bottom center of the collecting tank 12; for example, Fig.13 The ninth pipeline 53 of the middle pipeline assembly passes through the through hole 1111 and is connected to the liquid outlet 122; Figure 8 The fourth pipeline 45 of the middle pipeline assembly can also pass through the perforation 1111 to connect with the liquid outlet 122. Of course, the liquid outlet 122 of the collecting tank 12 can be located at the bottom center thereof, and the corresponding pipeline can be connected to the liquid outlet 122 via a rotating connector to avoid pipeline entanglement.

[0105] Based on the above solution, the corresponding pipeline of the pipeline assembly is connected to the liquid outlet 122 and then passes through the perforation 1111 to avoid entanglement of the pipeline, which is beneficial to the flow of liquid in the pipeline.

[0106] The second aspect of the present application provides a slice collection system 100. The slice collection system 100 comprises a slice tank 20, a slicer, a pipeline assembly and the slice collection assembly 10 of the first aspect, wherein the slice tank 20 contains liquid, a cutter 30 of the slicer is in the slice tank 20 to slice the sample, and the pipeline assembly is connected between the slice tank 20, the collection tank 12 and the collection cover plate 13 to deliver the slices cut by the slicer into the collection holes 141 of the porous plate 14.

[0107] The shape of the slicing groove 20 is not limited in the present application. For example, the shape of the slicing groove 20 can be circular, elliptical, polygonal, or other shapes. When the slicing groove 20 is a polygon, the slicing groove 20 can be rectangular, pentagonal, hexagonal, etc. The depth of the slicing groove 20 can be designed as needed.

[0108] The main structure of the slicer is not shown, only the cutter 30 is shown. The slicer can use a more mature vibration slicer in the prior art. Vibration slices are not only simple, fast, and efficient, but also can ensure the flatness and cutting accuracy of the slices.

[0109] The slice collection system 100 adopts any one or more embodiments of the slice collection component 10 described above, and thus has the beneficial effects of the above embodiments, which will not be described one by one here.

[0110] Please also refer to Figures 7 to 9In some embodiments, the pipeline assembly includes a four-way pipe 41, a first pipeline 42, a second pipeline 43, a third pipeline 44, a fourth pipeline 45, a fifth pipeline 46, a sixth pipeline 47, a first drive pump 48 and a second drive pump 49. The four-way pipe 41 includes a body 411 and a first interface 412, a second interface 413, a third interface 414 and a fourth interface 415 provided on the body 411; the two ends of the first pipeline 42 are respectively connected to the slicing groove 20 and the first interface 412, and the two ends of the second pipeline 43 are respectively connected to the second interface 413 and the inlet of the first drive pump 48 The two ends of the third pipeline 44 are respectively connected to the outlet of the first driving pump 48 and the slicing groove 20; the fourth pipeline 45 is respectively connected to the liquid outlet 122 and the inlet of the second driving pump 49, the fifth pipeline 46 is respectively connected to the outlet of the second driving pump 49 and the third interface 414, and the sixth pipeline 47 is respectively connected to the fourth interface 415 and the liquid inlet 131; a filter is provided at the second interface 413, and valves are provided on the first pipeline 42, the second pipeline 43, the fifth pipeline 46 and the sixth pipeline 47; wherein the first driving pump 48 and the second driving pump 49 are independently arranged or integrated.

[0111] The first pipeline 42 , the second pipeline 43 , the fifth pipeline 46 and the sixth pipeline 47 are all provided with valves. Specifically, the first pipeline 42 is provided with a first valve 421 , the second pipeline 43 is provided with a second valve 431 , the fifth pipeline 46 is provided with a third valve 461 , and the sixth pipeline 47 is provided with a fourth valve 471 .

[0112] Among them, the slicing groove 20, the first pipeline 42, the four-way 41, the second pipeline 43, the first drive pump 48 and the third pipeline 44 form a first cycle; the fourth pipeline 45, the second drive pump 49, the fifth pipeline 46, the four-way 41, the sixth pipeline 47, and the slice collecting assembly 10 form a second cycle.

[0113] Optionally, at least one of the first pipeline 42 , the second pipeline 43 , the third pipeline 44 , the fourth pipeline 45 , the fifth pipeline 46 and the sixth pipeline 47 is a hose to facilitate the connection between the components of the slice collection system 100 .

[0114] Specifically, the cutter 30 cuts the sample in the slice tank 20 to form a slice, for example, the slice thickness is 100-300um, the first valve 421 and the second valve 431 are opened, and the third valve 461 and the fourth valve 471 are closed; under the drive of the first driving pump 48, the liquid drives the slice to enter the spool 41 through the first pipeline 42, and the slice is intercepted by the filter in the spool 41 and temporarily stored in the spool 41, and the liquid re-enters the slice tank 20 through the second pipeline 43, the first driving pump 48, and the third pipeline 44. Afterwards, the first valve 421 and the second valve 431 are closed, and the third valve 461 and the fourth valve 471 are opened; under the drive of the second driving pump 49, the liquid in the collection tank 12 enters the spool 41 through the fourth pipeline 45, the second driving pump 49, and the fifth pipeline 46 and takes away the slice intercepted by the filter, and the slice enters the collection hole 141 of the porous plate 14 in the collection tank 12 through the sixth pipeline 47 with the liquid. Then, the first driving device 11 drives the collecting tank 12 to rotate to collect the next slice, and the cycle is repeated until all the collecting holes 141 of the porous plate 14 have slices.

[0115] The first drive pump 48 and the second drive pump 49 are independently arranged, which means that the first drive pump 48 and the second drive pump 49 are arranged separately, and the first drive pump 48 and the second drive pump 49 are driven to work by different drive devices respectively; the first drive pump 48 and the second drive pump 49 are integrated, which means that the first drive pump 48 and the second drive pump 49 are fixed together, and the first drive pump 48 and the second drive pump 49 are driven to work by the same drive device.

[0116] Optionally, the first driving pump 48 and the second driving pump 49 are both peristaltic pumps.

[0117] The cross 41 is fixed on the second bracket 17 via a second connecting arm.

[0118] Please refer to Fig. 9 In some embodiments, at least one of the first interface 412 , the second interface 413 , the third interface 414 and the fourth interface 415 is detachably connected to the body 411 .

[0119] It can be understood that one of the first interface 412, the second interface 413, the third interface 414 and the fourth interface 415 can be detachably connected to the main body 411; or, two of the first interface 412, the second interface 413, the third interface 414 and the fourth interface 415 can be detachably connected to the main body 411; or, three of the first interface 412, the second interface 413, the third interface 414 and the fourth interface 415 can be detachably connected to the main body 411; or, the first interface 412, the second interface 413, the third interface 414 and the fourth interface 415 can all be detachably connected to the main body 411.

[0120] When one of the first interface 412, the second interface 413, the third interface 414 and the fourth interface 415 is detachably connected to the body 411, optionally, the third interface 414 is detachably connected to the body 411, and the inner end of the second interface 413 is provided with a filter installation portion 4131, and after the third interface 414 is removed, it is convenient to disassemble and assemble the filter, which is conducive to the replacement of the filter. Of course, the second interface 413 can also be directly detachably connected to the body 411.

[0121] The present application does not limit the manner of detachable connection. For example, the detachable connection may be a threaded connection, a snap connection, a fastener connection, etc.

[0122] Please also refer to Fig.10 and Fig.11 In some embodiments, the pipeline assembly includes a seventh pipeline 51, an eighth pipeline 52, a ninth pipeline 53, a tenth pipeline 54, a third drive pump 55 and a fourth drive pump 56, wherein the two ends of the seventh pipeline 51 are respectively connected to the slicing slot 20 and the inlet of the third drive pump 55, the two ends of the eighth pipeline 52 are respectively connected to the outlet of the third drive pump 55 and the liquid inlet 131, the two ends of the ninth pipeline 53 are respectively connected to the liquid outlet 122 and the inlet of the fourth drive pump 56, and the two ends of the tenth pipeline 54 are respectively connected to the outlet of the fourth drive pump 56 and the slicing slot 20; wherein the third drive pump 55 and the fourth drive pump 56 are independently or integratedly arranged.

[0123] Among them, the slice tank 20, the seventh pipeline 51, the third driving pump 55, the eighth pipeline 52, the slice collecting assembly 10, the ninth pipeline 53, the fourth driving pump 56 and the tenth pipeline 54 form a third cycle.

[0124] Optionally, at least one of the seventh pipeline 51 , the eighth pipeline 52 , the ninth pipeline 53 and the tenth pipeline 54 is a hose to facilitate the connection between the components of the slice collection system 100 .

[0125] Specifically, the cutter 30 cuts the sample in the slice tank 20 to form a slice, for example, the slice thickness is 100-300um, and the third drive pump 55 and the fourth drive pump 56 work simultaneously; driven by the third drive pump 55, the liquid drives the slice through the seventh pipeline 51, the third drive pump 55 and the eighth pipeline 52 into the collection hole 141 of the porous plate 14 in the collection tank 12, and the slice stays in the collection hole 141; driven by the fourth drive pump 56, the liquid in the collection tank 12 re-enters the slice tank 20 through the ninth pipeline 53, the fourth drive pump 56, and the tenth pipeline 54. Then, the first drive device 11 drives the collection tank 12 to rotate to collect the next slice. This cycle is repeated until there are slices in the multiple collection holes 141 of the porous plate 14.

[0126] The third drive pump 55 and the fourth drive pump 56 are independently arranged, which means that the third drive pump 55 and the fourth drive pump 56 are arranged separately, and the third drive pump 55 and the fourth drive pump 56 are driven to work by different drive devices respectively; the third drive pump 55 and the fourth drive pump 56 are integrated, which means that the third drive pump 55 and the fourth drive pump 56 are fixed together, and the third drive pump 55 and the fourth drive pump 56 are driven to work by the same drive device.

[0127] Optionally, the third driving pump 55 and the fourth driving pump 56 are both peristaltic pumps.

[0128] In some embodiments, at least one pipeline of the pipeline assembly is provided with a cooler 60 for cooling the liquid.

[0129] Optionally, a cooler 60 for cooling the liquid is provided on one of the pipelines of the pipeline assembly. Figure 7 In the embodiment, a cooler 60 is provided on the third pipeline 44; of course, a cooler 60 may also be provided on the first pipeline 42, the second pipeline 43, the fourth pipeline 45, the fifth pipeline 46 or the sixth pipeline 47. Alternatively, Fig.10 In the embodiment, a cooler 60 is provided on the eighth pipeline 52 ; of course, a cooler 60 may also be provided on the seventh pipeline 51 , the ninth pipeline 53 or the tenth pipeline 54 .

[0130] Optionally, the cooler 60 includes a cooling body and a cooling channel arranged in the cooling body. The cooling body is made of metal and has good thermal conductivity, which is conducive to cooling. The cooling channel is a spiral channel to increase the flow time of the liquid in the cooling channel, thereby effectively cooling the liquid.

[0131] Based on the above scheme, the liquid is always kept within the set temperature during the slice collection process to avoid the impact of excessive temperature on the slices, thereby ensuring the activity and integrity of the slices in subsequent experiments.

[0132] In other embodiments, when the slice collection system 100 is in an environment with a set temperature, the cooler 60 may not be provided.

[0133] In some embodiments, the pipeline assembly includes a detection pipeline connected to the liquid inlet 131, and the slice collection system 100 also includes a sensor 70 corresponding to the detection pipeline, and the sensor 70 is used to detect whether the slice passes through the detection pipeline.

[0134] Optionally, the detection pipeline is a transparent pipeline, and the sensor 70 is a transmitting and receiving sensor, such as a laser sensor, an infrared sensor, etc. When the slice passes through the detection pipeline, the slice will block the light to confirm that the slice passes through the detection pipeline, and then the sensor 70 will send a signal to collect the next slice. The detection pipeline can be a hard tube or a soft tube.

[0135] Optionally, the sensor 70 is fixed to the second bracket 17 via a third connecting arm.

[0136] exist Figure 8 In the embodiment, the detection pipeline is the sixth pipeline 47. Optionally, the curved portion of the fifth pipeline 46 should be farther away from the sensor 70 to avoid that the curved position cannot be completely filled with liquid, thereby affecting the detection of the sensor 70. Fig.11 In the figure, the detection pipeline is a part of the eighth pipeline 52. Optionally, the curved portion of the eighth pipeline 52 should be farther away from the sensor 70 to avoid the bending position not being completely filled with liquid, which would affect the detection of the sensor 70.

[0137] In other embodiments, the sensor 70 may not be provided, and human eyes may be used to observe whether slices enter the collecting holes 141 of the porous plate 14. In this case, the collecting cover plate 13 needs to be designed as a transparent cover plate.

[0138] Please refer to Figure 7 and Figure 8 In some embodiments, a pusher 80 for pushing the sample into the slice slot 20 is provided at the bottom of the slice slot 20 .

[0139] Optionally, the pusher 80 includes a sleeve and a push rod slidably mounted in the sleeve, the sleeve is used to install the sample, and the push rod gradually pushes the sample out of the sleeve and into the slice groove 20. The push rod can be pushed by a driving mechanism or manually.

[0140] Based on the above solution, since the sample is in the pusher 80 and is gradually pushed into the slicing groove 20, the sample hardly occupies the volume of the slicing groove 20, which is beneficial to reducing the size of the slicing groove 20 and also avoids the sample being exposed to the outside for a long time.

[0141] In other embodiments, the pusher 80 may not be provided, and the sample may be placed directly on the bottom of the slicing groove 20 .

[0142] Please refer to Fig.12 In some embodiments, the slice collection system 100 also includes a culture device 90, which includes a culture tray 91 and a culture cover 92 for covering the culture tray 91, and the culture tray 91 has a plurality of culture holes 911 arranged at intervals along its circumference, and the plurality of culture holes 911 are respectively arranged corresponding to the plurality of collection holes 141.

[0143] Among them, after the multiple collection holes 141 enter the multiple culture holes 911 respectively, different drugs can be applied to the multiple slices to achieve the purpose of drug screening.

[0144] Optionally, a second annular recess 912 is provided on the top of the culture tray 91, and a second annular flange 921 is provided on the culture cover 92; when the culture cover 92 is covered on the culture tray 91, the second annular flange 921 is inserted into the second annular recess 912 to prevent the culture cover 92 from moving horizontally.

[0145] Optionally, a first central hole 913 is provided in the middle of the culture tray 91, and a second central hole 922 is provided in the middle of the culture cover plate 92, so as to meet the air requirements of the tissue cells of the slices. Of course, the middle of the culture tray 91 and the middle of the culture cover plate 92 can also be set to a grid structure.

[0146] Based on the above scheme, slice samples can be directly used for in vitro cell culture, which simplifies the operation process and facilitates drug screening and related experiments.

[0147] The slice collection system provided in the embodiment of the present application can efficiently complete the tissue sample slicing, recovery and culture process in anticancer drug screening. By automatically completing slicing, transmission, packaging and culture, the standardization of sample processing is achieved, human errors are reduced, the integrity of the sample is guaranteed, the reliability and stability of the experimental results are ensured, and the screening process of anticancer drugs is accelerated.

[0148] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application, and should all be included in the protection scope of the present application.

Claims

1. A slice collection assembly, characterized in that: It includes a first driving device, a collecting tank, a collecting cover and a porous plate, the first driving device is connected to the collecting tank and drives the collecting tank to rotate relative to the collecting cover, the collecting tank has an upward opening, and the collecting tank is provided with a liquid outlet for connecting to the pipeline assembly; the collecting cover covers the opening, and the collecting cover is provided with a liquid inlet for connecting to the pipeline assembly; the porous plate is detachably installed in the collecting tank and can rotate with the collecting tank, the porous plate is provided with a plurality of collecting holes arranged at intervals along the rotation direction thereof, and the bottom wall and / or the side wall of the collecting hole are provided with hollowing; during the rotation of the porous plate with the collecting tank, the plurality of collecting holes can be aligned with the liquid inlet respectively.

2. The slice collection assembly according to claim 1, characterized in that: A first annular recess is provided on the top of the collecting trough, and a first annular flange is provided on the edge of the collecting cover plate extending into the first annular recess.

3. The slice collection assembly according to claim 2, characterized in that: A sealing liquid is arranged in the first annular recess.

4. The slice collection assembly according to claim 1, characterized in that: The bottom of the collecting hole is provided with a plurality of supporting protrusions for supporting the slices.

5. The slice collection assembly according to claim 1, characterized in that: The top of the collecting trough is provided with an anti-rotation recess, and the porous plate is provided with an anti-rotation protrusion that cooperates with the anti-rotation recess; or, the top of the collecting trough is provided with an anti-rotation protrusion, and the porous plate is provided with an anti-rotation recess that cooperates with the anti-rotation protrusion.

6. The slice collection assembly according to any one of claims 1 to 5, characterized in that: The slice collection assembly further includes a first bracket, and the first driving device drives the collection tank to rotate through the first bracket; And / or, the slice collection assembly further includes a second driving device and a second bracket, and the second driving device drives the collection cover plate to rise and fall via the second bracket.

7. The slice collection assembly according to any one of claims 1 to 5, characterized in that: The first driving device includes a first driving motor and a reducer connected to the first driving motor, wherein the reducer has a through hole for a corresponding pipeline of the pipeline assembly to pass through.

8. A slice collection system, characterized in that: It comprises a slicing groove, a slicer, a piping assembly and a slice collecting assembly as described in any one of claims 1 to 7, wherein the cutter of the slicer is located in the slicing groove, and the piping assembly is connected between the slicing groove, the collecting groove and the collecting cover plate to deliver the slices cut by the slicer into the collecting holes of the porous plate.

9. The slice collection system according to claim 8, characterized in that: The pipeline assembly includes a four-way pipe, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline, a fifth pipeline, a sixth pipeline, a first driving pump and a second driving pump. The four-way pipe includes a body and a first interface, a second interface, a third interface and a fourth interface provided on the body; two ends of the first pipeline are respectively connected to the slicing groove and the first interface, two ends of the second pipeline are respectively connected to the second interface and the inlet of the first driving pump, and two ends of the third pipeline are respectively connected to the outlet of the first driving pump and the slicing groove; The fourth pipeline is respectively connected to the liquid outlet and the inlet of the second driving pump, the fifth pipeline is respectively connected to the outlet of the second driving pump and the third interface, and the sixth pipeline is respectively connected to the fourth interface and the liquid inlet; a filter is provided at the second interface, and valves are provided on the first pipeline, the second pipeline, the fifth pipeline and the sixth pipeline; wherein the first driving pump and the second driving pump are independently arranged or integrated.

10. The slice collection system according to claim 9, characterized in that: At least one of the first interface, the second interface, the third interface and the fourth interface is detachably connected to the body.

11. The slice collection system according to claim 8, characterized in that: The pipeline assembly includes a seventh pipeline, an eighth pipeline, a ninth pipeline, a tenth pipeline, a third drive pump and a fourth drive pump, wherein the two ends of the seventh pipeline are respectively connected to the slicing groove and the inlet of the third drive pump, the two ends of the eighth pipeline are respectively connected to the outlet of the third drive pump and the liquid inlet, the two ends of the ninth pipeline are respectively connected to the liquid outlet and the inlet of the fourth drive pump, and the two ends of the tenth pipeline are respectively connected to the outlet of the fourth drive pump and the slicing groove; wherein the third drive pump and the fourth drive pump are independently or integratedly arranged.

12. The slice collection system according to any one of claims 9 to 11, characterized in that: At least one pipeline of the pipeline assembly is provided with a cooler for cooling liquid.

13. The slice collection system according to any one of claims 8 to 11, characterized in that: The pipeline assembly includes a detection pipeline connected to the liquid inlet, and the slice collection system also includes a sensor corresponding to the detection pipeline, and the sensor is used to detect whether the slice passes through the detection pipeline.

14. The slice collection system according to any one of claims 8 to 11, characterized in that: A pusher for pushing the sample into the slice groove is provided at the bottom of the slice groove.

15. The slice collection system according to any one of claims 8 to 11, characterized in that: The slice collection system also includes a culture device, which includes a culture tray and a culture cover for covering the culture tray. The culture tray has a plurality of culture holes arranged at intervals along its circumference, and the plurality of culture holes are respectively arranged corresponding to the plurality of collection holes.

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