ODR microbial agent for efficiently treating organic solid waste as well as preparation method and application of ODR microbial agent

Abstract

The invention discloses an ODR microbial agent for efficiently treating organic solid waste as well as a preparation method and application of the ODR microbial agent, and belongs to the technical field of environmental protection. The ODR microbial agent for efficiently treating the organic solid waste comprises core functional bacteria, auxiliary enzymes, a stabilizer and a carrier, the core functional bacteria comprise mesophile organisms, thermophilic bacteria and microorganisms in the decomposition period, and bacillus subtilis and pseudomonas aeruginosa are selected as the mesophile organisms. According to the ODR microbial agent for efficiently treating the organic solid waste and the preparation method and application of the ODR microbial agent, the ODR microbial agent contains mesophilic organisms, thermophilic bacteria and microorganisms in the decomposition period, a complete microbial community is formed and can cover all stages of the composting process, the mesophilic organisms rapidly decompose easily-degraded organic matter in the initial stage of composting, and the composting time is shortened; the high-temperature bacteria accelerate the decomposition of complex organic matters in the high-temperature stage, and the microorganisms in the decomposition stage promote the formation of humus in the later stage.

Description

Technical Field

[0001] The present invention relates to the field of environmental protection technology, and in particular to an ODR microbial agent for efficiently treating organic solid waste, and a preparation method and application thereof. Background Art

[0002] With the acceleration of global urbanization, the amount of organic solid waste generated has increased year by year. Traditional treatment methods such as landfill and incineration have problems such as waste of resources and environmental pollution. As an environmentally friendly treatment method, biological treatment has the advantages of harmlessness, reduction and resource utilization, but the low efficiency, long cycle and high cost of traditional biological treatment limit its wide application. Therefore, the development of efficient microbial agents to accelerate the decomposition and transformation of organic solid waste is crucial to improving the effect of biological treatment. Summary of the invention

[0003] The object of the present invention is to provide an ODR microbial agent for efficiently treating organic solid waste, and a preparation method and application thereof. The ODR microbial agent includes mesophilic organisms, thermophilic bacteria and mature-stage microorganisms to form a complete microbial community that can cover all stages of the composting process. Mesophilic organisms quickly decompose easily degradable organic matter in the early stage of composting, thermophilic bacteria accelerate the decomposition of complex organic matter in the high temperature stage, and mature-stage microorganisms promote the formation of humus in the later stage. This multi-level microbial combination ensures the efficient degradation of organic matter, shortens the composting cycle, and solves the problems raised in the above-mentioned background technology.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an ODR microbial agent for efficiently treating organic solid waste, comprising core functional bacteria, auxiliary enzymes, stabilizers and carriers, wherein the core functional bacteria include mesophilic organisms, thermophilic bacteria and mature-stage microorganisms, wherein the mesophilic organisms are selected from Bacillus subtilis and Pseudomonas aeruginosa, the thermophilic bacteria are selected from Clostridium thermocellum and Bacillus stearothermophilus, the mature-stage microorganisms are selected from Schizophyllum and Streptomyces, and the carriers are selected from rice husks and sodium alginate.

[0005] Preferably, the auxiliary enzymes include at least one of cellulase, hemicellulase, pectinase and protease.

[0006] Preferably, the stabilizer includes at least one of glycerol, phosphate buffer and vitamin C.

[0007] Preferably, the mesophilic organisms further include at least one of Candida and Gram-negative bacteria.

[0008] Another technical problem to be solved by the present invention is to provide a method for preparing an ODR microbial agent for efficiently treating organic solid waste, comprising the following steps:

[0009] Step 1: Pre-culture Bacillus subtilis, Pseudomonas aeruginosa, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces separately to ensure that they fully proliferate at their respective optimal temperatures until OD600 reaches 0.5-1.0;

[0010] Step 2: Bacillus subtilis, Pseudomonas aeruginosa, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces are mixed with pre-cultured bacterial liquid in a mass percentage ratio of (18-25):(12-18):(12-18):(12-18):(12-18):(8-12), and auxiliary enzymes are added, stirred evenly and inoculated into the optimized culture medium;

[0011] Step 3: Fixing the mixed microbial liquid onto rice husks by embedding technology to prepare a granular bacterial agent;

[0012] Step 4: Use vacuum packaging to ensure the activity of the bacterial agent during long-term storage.

[0013] Preferably, the Bacillus subtilis, Pseudomonas aeruginosa, Schizophyllum and Streptomyces are cultured at 30°C for 48 hours, and Clostridium thermocellum and Bacillus stearothermophilus are cultured at 60°C-70°C for 72 hours.

[0014] Preferably, the specific method of step 2 is as follows: the prepared culture medium is autoclaved at 121° C. for 15 minutes to ensure a sterile environment, a sterile pipette is used to draw the Bacillus subtilis preculture solution, and slowly added to a sterile container, and then the Pseudomonas aeruginosa preculture solution, the thermocellum Clostridium preculture solution, the thermostearothermophilus Bacillus preculture solution, the Schizophyllum preculture solution and the Streptomyces preculture solution are drawn in sequence, and slowly added to the same container, the auxiliary enzymes are directly added to the mixed bacterial solution to ensure that the enzymes are in full contact with the microorganisms, and a sterile glass rod or a magnetic stirrer is used to gently stir to ensure that each strain is fully mixed, and violent stirring is avoided to avoid destroying the bacterial structure.

[0015] Preferably, the specific method of step three is as follows: the rice husk particles after the crushing process are between 0.5-2 mm, so as to facilitate subsequent operations, the rice husk is washed with distilled water several times to remove impurities, and then dried at 105°C for 24 hours to ensure that there is no residual moisture, and then it can be sterilized at 121°C for 30 minutes to ensure a sterile environment, prepare a sodium alginate solution, weigh 2-3g of sodium alginate, add it to 100mL of distilled water, stir until completely dissolved, form a 2%-3% sodium alginate solution, and carry out Sterilization, add a stabilizer to the sodium alginate solution, add the mixed microbial culture to the sodium alginate solution, gently stir to ensure that the culture is evenly dispersed, drop the mixed solution drop by drop into a 10% calcium chloride solution to form gel beads, let it stand for 30 minutes to allow the gel beads to fully solidify, then wash with sterile distilled water to remove excess calcium chloride, add the gel beads to the pretreated rice husks, gently stir to evenly distribute the gel beads on the surface of the rice husks, separate the rice husks that have adsorbed the gel beads, and then dry them at room temperature or low temperature.

[0016] Another technical problem to be solved by the present invention is to provide an application of an ODR microbial agent for efficiently treating organic solid waste, which is used for composting of organic solid waste.

[0017] Preferably, the application method of the ODR microbial agent for efficiently treating organic solid waste comprises the following steps:

[0018] The collected organic solid waste is preliminarily crushed, classified and impurities are removed. Bacteria needles, peanut shells and crop straw crushed materials are prepared as auxiliary materials. The organic solid waste, auxiliary materials and ODR microbial agents are evenly mixed in a ratio of 1:1:0.1 and piled into strips with a height of about 1.5 meters and a width of 2-6 meters. The moisture content of the mixed pile is 40-60%. The mesophilic microorganisms in the compost pile use soluble and easily degradable organic matter as a source of nutrition and energy, proliferate rapidly, and release heat energy, causing the temperature of the compost pile to rise continuously, gradually increasing the temperature to 40-60℃. When the temperature of the compost pile rises to 60-70℃, it enters the high temperature stage, and the roundworm eggs and large worms in the compost pile die. Pathogens such as Enterobacteriaceae and grass seeds are inactivated, and heavy metals are effectively degraded. At the end of fermentation, only some difficult-to-decompose organic matter and newly formed humus are left. At this time, the activity of microorganisms decreases, the calorific value decreases, and the temperature drops. At this time, mesophilic microorganisms are dominant again, and further decompose the remaining difficult-to-decompose organic matter. The humus continues to increase and tends to stabilize. At this time, the fermentation enters the mature stage, and the pile is turned over every two days until the temperature gradually drops below 30°C and the oxygen demand decreases. At this time, only natural ventilation is required. After the fermentation is completed, the product is crushed and screened to remove large pieces of material that have not been completely decomposed, and granulation is carried out to finally obtain high-quality organic fertilizer products that meet national standards.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The invention discloses an ODR microbial agent for efficiently treating organic solid waste, and a preparation method and application thereof. The ODR microbial agent comprises mesophilic organisms, thermophilic bacteria and mature-stage microorganisms, forming a complete microbial community, which can cover all stages of the composting process. Mesophilic organisms rapidly decompose easily degradable organic matter in the early stage of composting, thermophilic bacteria accelerate the decomposition of complex organic matter in the high-temperature stage, and mature-stage microorganisms promote the formation of humus in the later stage. This multi-level microbial combination ensures the efficient degradation of organic matter and shortens the composting cycle. By adding auxiliary enzymes, the degradation capacity of complex organic substrates is further enhanced, so that the decomposition of organic matter is more thorough and the residue of undegraded substances is reduced. Sodium alginate is used as a carrier, and gel beads are formed by cross-linking with calcium chloride to fix the microorganisms on rice husks. This embedding technology not only provides a stable microenvironment for microorganisms, but also effectively prevents the influence of the external environment and maintains the long-term activity of microorganisms. At the same time, rice husk, as a natural carrier, has good air permeability and water retention, which helps microorganisms to continue to function during the composting process. Due to the presence of thermophilic bacteria, the temperature can quickly rise to 60-70°C during the composting process and maintain a long period of high temperature, which effectively inactivates pathogens and weed seeds in the compost pile and reduces the risk of secondary pollution. This ODR microbial agent can not only be used for the treatment of common organic solid wastes such as kitchen waste, agricultural waste, and garden waste, but also can be used for the treatment of complex organic wastes such as sludge and livestock and poultry manure. By adjusting auxiliary materials and operating parameters, it can flexibly respond to different types of organic solid wastes and meet diverse market needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a flow chart for preparing the ODR microbial agent. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Embodiment 1:

[0024] Cultivation of Bacillus subtilis, Pseudomonas aeruginosa, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum, Streptomyces, Candida, and Gram-negative bacteria

[0025] Bacillus subtilis, Pseudomonas aeruginosa, Schizophyllum, Streptomyces, Candida and Gram-negative bacteria were cultured in culture medium at 30°C for 48 hours until OD600 reached 0.5-1.0. The composition of the pre-culture medium was as follows:

[0026] Glucose: 20g / L

[0027] Wheat bran: 10g / L

[0028] Yeast extract: 5g / L

[0029] Ammonium nitrate: 2g / L

[0030] Potassium dihydrogen phosphate: 1g / L

[0031] Magnesium sulfate: 0.5g / L

[0032] Trace element solution: 1mL / L

[0033] Adjust the moisture to 40%-60%, pH value: adjust to 6.0-7.0.

[0034] Clostridium thermocellum and Bacillus stearothermophilus were cultured at 60°C-70°C for 72 hours until OD600 reached 0.5-1.0. The composition of the culture medium was as follows:

[0035] Cellulose powder: 20g / L

[0036] Hemicellulose powder: 10g / L

[0037] Peptone: 5g / L

[0038] Ammonia: 2g / L

[0039] Potassium dihydrogen phosphate: 1g / L

[0040] Magnesium sulfate: 0.5g / L

[0041] Trace element solution: 1mL / L

[0042] Adjust the moisture to 40%-60%, pH value: adjust to 6.5-7.5.

[0043] Embodiment 2:

[0044] See also Figure 1 , an ODR microbial agent for efficiently treating organic solid waste, prepare the following materials:

[0045] Core functional bacteria

[0046] Mesophilic organisms: Bacillus subtilis, Pseudomonas aeruginosa;

[0047] Thermobacteria: Clostridium thermocellum, Bacillus stearothermophilus;

[0048] Microorganisms in the decomposition stage: Schizophyllum, Streptomyces;

[0049] Auxiliary enzymes

[0050] Cellulase: 0.1g / L, hemicellulase: 0.1g / L, pectinase: 0.1g / L;

[0051] Stabilizer

[0052] Glycerol: final concentration 1%, phosphate buffer: 50 mM;

[0053] Carrier

[0054] Rice husk: particle size 0.5-2mm, sodium alginate: 2%-3% w / v;

[0055] The above materials are used to prepare the ODR microbial agent, and the method is as follows:

[0056] The pre-cultured bacterial solution of Bacillus subtilis, Pseudomonas aeruginosa, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces was mixed in a mass percentage of 22:16:16:16:16:10, cellulase, hemicellulase and protease were added, and the mixture was gently stirred to ensure that the enzymes were in full contact with the microorganisms. The rice husk was washed with distilled water several times to remove impurities, and then dried at 105°C for 24 hours to ensure that no moisture remained, and then autoclaved at 121°C for 30 minutes to ensure a sterile environment. 3 g of sodium alginate was weighed and added Add to 100mL distilled water, stir until completely dissolved to form a 3% sodium alginate solution, and sterilize, add the mixed microbial bacterial solution to the sodium alginate solution containing vitamin C and phosphate buffer, stir gently to ensure that the bacterial solution is evenly dispersed, drip the mixed solution drop by drop into a 10% calcium chloride solution to form gel beads, let stand for 30 minutes to fully solidify the gel beads, then wash with sterile distilled water to remove excess calcium chloride, add the gel beads to the pretreated rice husk, stir gently to evenly distribute the gel beads on the surface of the rice husk. Separate the rice husk adsorbed with gel beads, and then dry at room temperature or low temperature to prepare the ODR microbial agent of Example 2.

[0057] The collected kitchen waste is preliminarily crushed, sorted and impurities are removed, and peanut shells and crop straw crushed materials are prepared as auxiliary materials. Organic solid waste: auxiliary materials: ODR microbial agents are mixed evenly in a ratio of 1:1:0.1, and then stacked into strips with a height of about 1.5 meters and a width of 2 meters. The moisture content of the mixed pile is 40%-60%. Fermentation begins. The temperature gradually rises to 40-60°C for 3 days in the initial fermentation. After entering the high temperature stage, the temperature is maintained at 60-70°C for 7 days, during which pathogens and heavy metals are effectively degraded. Then it enters the composting stage, which lasts for 20 days, and the pile is turned over every two days until the temperature gradually drops below 30°C. After the fermentation is completed, the product is crushed and screened, the large pieces of material that are not completely decomposed are removed, and granulation is carried out to finally obtain high-quality organic fertilizer products that meet national standards.

[0058] Embodiment three:

[0059] Prepare the following materials: Core functional bacteria

[0060] Mesophilic organisms: Bacillus subtilis, Pseudomonas aeruginosa, Candida

[0061] Thermobacteria: Clostridium thermocellum, Bacillus stearothermophilus

[0062] Microorganisms in the mature stage: Schizophyllum, Streptomyces

[0063] Auxiliary enzymes

[0064] Cellulase: 0.2g / L, Hemicellulase: 0.2g / L, Protease: 0.1g / L

[0065] Stabilizer

[0066] Vitamin C: final concentration 0.2%, phosphate buffer: 50 mM;

[0067] Carrier

[0068] Rice husk: particle size 0.5-2mm, sodium alginate: 3% w / v

[0069] The above materials were used to prepare the ODR microbial agent: the pre-cultured bacterial solution of Bacillus subtilis, Pseudomonas aeruginosa, Candida, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces were mixed in a weight percentage of 22:16:10:16:16:16:10, auxiliary enzymes were added, and the mixture was gently stirred to ensure that the enzymes were in full contact with the microorganisms. The rice husk was washed with distilled water several times to remove impurities, and then dried at 105°C for 24 hours to ensure that no moisture remained, and then dried at 121°C. Sterilize by high pressure for 30 minutes to ensure a sterile environment, weigh 3g of sodium alginate, add it to 100mL of distilled water, stir until completely dissolved, form a 3% sodium alginate solution, and sterilize, add the mixed microbial culture to the sodium alginate solution containing vitamin C and phosphate buffer, stir gently to ensure that the culture is evenly dispersed, drip the mixed solution drop by drop into a 10% calcium chloride solution to form gel beads, let stand for 30 minutes to fully solidify the gel beads, then wash with sterile distilled water to remove excess calcium chloride. Add the gel beads to the pretreated rice husk, stir gently to evenly distribute the gel beads on the surface of the rice husk, separate the rice husk adsorbed with the gel beads, and then dry at room temperature or low temperature to prepare the ODR microbial agent of Example 3.

[0070] The collected garden waste, mainly leaves and branches, is preliminarily crushed, classified and impurities are removed, and peanut shells and crop straw crushed materials are prepared as auxiliary materials. Organic solid waste: auxiliary materials: ODR microbial agents are mixed evenly in a ratio of 1:1:0.1 and piled into strips with a height of about 1.5 meters and a width of 5 meters. The moisture content of the pile after mixing is 40%-60%. After the initial fermentation for 3 days, the temperature gradually rises to 40-60℃. After entering the high temperature stage, the temperature is maintained at 60-70℃ for 10 days, during which pathogens and heavy metals are effectively degraded. Then it enters the mature stage, which lasts for 16 days, and the pile is turned over every two days until the temperature gradually drops below 30℃. After the fermentation is completed, the product is crushed and screened, the large pieces of material that are not completely decomposed are removed, and granulation is carried out to finally obtain high-quality organic fertilizer products that meet national standards.

[0071] Embodiment 4:

[0072] Prepare the following materials: Core functional bacteria

[0073] Mesophilic organisms: Bacillus subtilis, Pseudomonas aeruginosa, Gram-negative bacteria;

[0074] Thermobacteria: Clostridium thermocellum, Bacillus stearothermophilus;

[0075] Microorganisms in the decomposition stage: Schizophyllum, Streptomyces;

[0076] Auxiliary enzymes

[0077] Cellulase: 0.15g / L, hemicellulase: 0.15g / L, pectinase: 0.15g / L

[0078] Stabilizer

[0079] Glycerol: Final concentration 1.5%, Vitamin C: Final concentration 0.1%

[0080] Carrier

[0081] Rice husk: particle size 0.5-2 mm, sodium alginate: 2.5% w / v;

[0082] The above materials were used to prepare the ODR microbial agent: Bacillus subtilis, Pseudomonas aeruginosa, Gram-negative bacteria, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces were mixed in a pre-cultured bacterial solution in a weight percentage of 25:18:10:18:18:18:10, cellulase, hemicellulase and pectinase were added, and the mixture was gently stirred to ensure that the enzymes were in full contact with the microorganisms. The rice husk was washed with distilled water several times to remove impurities, and then dried at 105°C for 24 hours to ensure that there was no residual moisture. , then sterilize at 121℃ for 30 minutes to ensure a sterile environment, weigh 3g of sodium alginate, add it to 100mL of distilled water, stir until completely dissolved, form a 3% sodium alginate solution, and sterilize, add the mixed microbial culture to the sodium alginate solution containing vitamin C and glycerol, stir gently to ensure that the culture is evenly dispersed, drip the mixed solution drop by drop into a 10% calcium chloride solution to form gel beads, let stand for 30 minutes to fully solidify the gel beads, then wash with sterile distilled water to remove excess calcium chloride. Add the gel beads to the pretreated rice husks, stir gently to evenly distribute the gel beads on the surface of the rice husks, separate the rice husks adsorbed with the gel beads, and then dry at room temperature or low temperature to prepare the ODR microbial agent of Example 4.

[0083] The collected agricultural waste, mainly crop straw and livestock and poultry manure, is preliminarily crushed, classified and impurities are removed, and peanut shells and crop straw crushed materials are prepared as auxiliary materials. Organic solid waste: auxiliary materials: ODR microbial agents are mixed evenly in a ratio of 1:1:0.1 and stacked into strips with a height of about 1.5 meters and a width of 6 meters. The moisture content of the mixed pile is 40%-60%. After the initial fermentation for 3 days, the temperature gradually rises to 40-60℃. After entering the high temperature stage, the temperature is maintained at 60-70℃ for 10 days, during which pathogens and heavy metals are effectively degraded. Then it enters the mature stage, which lasts for 16 days, and the pile is turned over every two days until the temperature gradually drops below 30℃. After the fermentation is completed, the product is crushed and screened, the large pieces of material that are not completely decomposed are removed, and granulation is carried out to finally obtain high-quality organic fertilizer products that meet national standards.

[0084] The compost used in the above-mentioned Examples 2 to 4 was tested to obtain the following data:

[0085]

[0086] It can be concluded from the above table that the combination based on six microorganisms can provide higher organic matter and nutrient content, is suitable for the production of general organic fertilizers, and has good microbial activity and maturity. However, relatively speaking, the addition of Candida and Gram-negative bacteria can further improve the performance. The addition of Candida in Implementation 3 significantly increased the organic matter content and total nutrient content, especially in the decomposition of complex organic matter. In addition, Candida can accelerate the decomposition process, shorten the fermentation cycle, reduce greenhouse gas emissions, and increase fungal diversity in the soil. The addition of Gram-negative bacteria in Implementation 4 performed well in the decomposition of complex organic matter. In addition, Gram-negative bacteria can accelerate the decomposition process, shorten the fermentation cycle, reduce greenhouse gas emissions, and increase bacterial diversity in the soil.

[0087] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0088] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. An ODR microbial agent for efficiently treating organic solid waste, comprising core functional bacteria, auxiliary enzymes, stabilizers and carriers, characterized in that: The core functional bacteria include mesophilic organisms, thermophilic bacteria and mature microorganisms, among which the mesophilic organisms are Bacillus subtilis and Pseudomonas aeruginosa, the thermophilic bacteria are Clostridium thermocellum and Bacillus stearothermophilus, the mature microorganisms are Schizophyllum and Streptomyces, and the carriers are rice husks and sodium alginate.

2. The ODR microbial agent for efficiently treating organic solid waste according to claim 1, characterized in that: The auxiliary enzymes include at least one of cellulase, hemicellulase, pectinase and protease.

3. The ODR microbial agent for efficiently treating organic solid waste according to claim 1, characterized in that: The stabilizer includes at least one of glycerol, phosphate buffer and vitamin C.

4. The ODR microbial agent for efficiently treating organic solid waste according to claim 1, characterized in that: The mesophilic organisms further include at least one of Candida and Gram-negative bacteria.

5. A method for preparing an ODR microbial agent for efficiently treating organic solid waste according to any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1: Pre-culture Bacillus subtilis, Pseudomonas aeruginosa, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces separately to ensure that they fully proliferate at their respective optimal temperatures until OD600 reaches 0.5-1.0; Step 2: Bacillus subtilis, Pseudomonas aeruginosa, Clostridium thermocellum, Bacillus stearothermophilus, Schizophyllum and Streptomyces are mixed with pre-cultured bacterial liquid in a mass percentage ratio of (18-25):(12-18):(12-18):(12-18):(12-18):(8-12), and auxiliary enzymes are added, stirred evenly and inoculated into the optimized culture medium; Step 3: Fixing the mixed microbial liquid onto rice husks through embedding technology to prepare a granular bacterial agent; Step 4: Use vacuum packaging to ensure the activity of the bacterial agent during long-term storage.

6. The method for preparing the ODR microbial agent for efficiently treating organic solid waste according to claim 5, characterized in that: The Bacillus subtilis, Pseudomonas aeruginosa, Schizophyllum and Streptomyces are cultured at 30°C for 48 hours, and the Clostridium thermocellum and Bacillus stearothermophilus are cultured at 60°C-70°C for 72 hours.

7. The method for preparing the ODR microbial agent for efficiently treating organic solid waste according to claim 5, characterized in that: The specific method of step 2 is as follows: the prepared culture medium is sterilized at 121° C. for 15 minutes under high pressure to ensure a sterile environment, the Bacillus subtilis preculture solution is aspirated with a sterile pipette, and slowly added into a sterile container, and then the Pseudomonas aeruginosa preculture solution, the Clostridium thermocellum preculture solution, the Bacillus stearothermophilus preculture solution, the Schizophyllum preculture solution and the Streptomyces preculture solution are aspirated in sequence, and slowly added into the same container, the auxiliary enzymes are directly added into the mixed bacterial solution, and the mixture is gently stirred with a sterile glass rod or a magnetic stirrer.

8. The method for preparing the ODR microbial agent for efficiently treating organic solid waste according to claim 5, characterized in that: The specific method of step three is as follows: washing the rice husk with distilled water for several times to remove impurities, and then drying it at 105° C. for 24 hours to prepare a sodium alginate solution, adding a stabilizer to the sodium alginate solution, adding the mixed microbial culture liquid to the sodium alginate solution, gently stirring to ensure that the culture liquid is evenly dispersed, dripping the mixed liquid drop by drop into a 10% calcium chloride solution to form gel beads, standing for 30 minutes to allow the gel beads to fully solidify, and then washing with sterile distilled water to remove excess calcium chloride, adding the gel beads to the pretreated rice husk, gently stirring to allow the gel beads to be evenly distributed on the surface of the rice husk, separating the rice husk adsorbed with the gel beads, and then drying at room temperature or low temperature.

9. The use of the ODR microbial agent for efficiently treating organic solid waste according to any one of claims 1 to 4, characterized in that: Used for composting of organic solid waste.

10. The use of the ODR microbial agent for efficiently treating organic solid waste according to claim 9, characterized in that: The following steps are involved: The collected organic solid waste is preliminarily crushed, classified and impurities are removed. Bacteria needles, peanut shells and crushed crop straw are prepared as auxiliary materials. The organic solid waste, auxiliary materials and ODR microbial agents are evenly mixed in a ratio of 1:1:0.1 and stacked into strips with a height of about 1.5 meters and a width of 2-6 meters. The moisture content of the mixed pile is 40-60%. During this period, the temperature is gradually increased to 40-60°C. When the temperature of the compost pile rises to 60-70°C, it enters the high temperature stage, and the ascarid eggs, Escherichia coli and other pathogens and grass seeds in the compost pile are inactivated, and heavy metals are effectively degraded. At the end of the fermentation, the temperature drops, and mesophilic microorganisms dominate again, further decomposing the residual organic matter that is difficult to decompose. At this time, the fermentation enters the mature stage, and the pile is turned over every two days until the temperature gradually drops below 30°C and the oxygen demand decreases. At this time, only natural ventilation is required. After the fermentation is completed, the product is crushed and screened, and the large pieces of material that are not completely decomposed are removed, and granulation is carried out to finally obtain high-quality organic fertilizer products that meet national standards.

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