Specimen collecting device suitable for centrifugal bacterium collecting method

By designing a specimen collection device suitable for centrifugal bacteria collection method, including transparent sampling cup, automated inner cup and slide, the problem of low specimen processing efficiency in the prior art is solved, automated processing and efficient concentration are achieved, and detection efficiency and work safety are improved.

CN119827267APending Publication Date: 2025-04-15HUNAN TECH NEW MEDICAL SYST
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Patent Information

Application Number
CN202510101143.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-22
Publication Date
2025-04-15

AI Technical Summary

Technical Problem

During the existing specimen treatment before centrifugation, the specimen needs to undergo multiple transfers and manual addition of digestive fluid, resulting in low detection efficiency.

Method used

A specimen collection device suitable for centrifugal bacterial collection methods is designed, including a sampling cup, a cup lid, an inner cup and a slide made of transparent material. An inner cup is provided in the sampling cup, and the inner cup is prefilled with pretreatment liquid. The sealing cover of the inner cup is automatically opened during centrifugation to reduce manual operation. There is a substrate on the slide. After centrifugation, bacteria and cells are adsorbed by the substrate for easier subsequent detection.

Benefits of technology

Through the automated specimen processing and concentration process, the detection efficiency is significantly improved, the direct contact between the detector and the specimen is reduced, and the work safety is improved.

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Abstract

The invention discloses a specimen collecting device suitable for a centrifugal bacterium collecting method, and belongs to the field of medical detection, the specimen collecting device comprises a sampling cup, and a cup cover is arranged at a cup opening of the sampling cup; the cup cover comprises a cover body connected with the sampling cup and a base, the cover body is connected with the base through a connecting section, a through hole for the slide glass to pass through is formed between the cover body and the base, and a substrate for collecting bacteria and cells is arranged on the slide glass. The invention aims to provide the specimen collecting device suitable for the centrifugal bacterium collecting method, and the detection efficiency can be improved.
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Description

Technical Field

[0001] The invention relates to the field of medical detection, in particular to a specimen collection device suitable for a centrifugal bacteria collection method. Background Art

[0002] When conducting a test such as tuberculosis detection, you first need to collect the specimen, then add pre-treatment fluids such as digestive fluid to the specimen, and then centrifuge the specimen. After centrifugation, the subsequent testing steps can be started. In the existing pre-centrifugation processing, the specimen needs to be transferred multiple times and the digestive fluid needs to be manually added, which results in low detection efficiency. Summary of the invention

[0003] The purpose of the present invention is to solve the above problems and provide a specimen collection device suitable for the centrifugal bacteria collection method, which can improve the detection efficiency.

[0004] To achieve the above purpose, the technical solution adopted by the present invention is a specimen collection device suitable for the centrifugal bacteria collection method, which includes a sampling cup, and a cup cover is arranged at the cup mouth of the sampling cup; the cup cover includes a cover body connected to the sampling cup and a base, the cover body is connected to the base through a connecting section, and a through hole for a slide to pass through is arranged between the cover body and the base, and a substrate for collecting bacteria and cells is arranged on the slide. At the end of centrifugation, the bacteria and cells are adsorbed by the substrate to facilitate microscopic examination, etc., thereby improving the detection efficiency.

[0005] Furthermore, the sampling cup is made of a transparent material to facilitate patients and testers to observe the sample volume.

[0006] Furthermore, a scale is provided on the cup body of the sampling cup along its axial direction to facilitate the examinee and the testing personnel to accurately understand the sample volume.

[0007] Furthermore, in order to improve the detection efficiency and protect the detection personnel at the same time, an inner cup is provided in the sampling cup, and the inner cup contains the pretreatment liquid, and a sealing cover which can be automatically opened during centrifugation is provided at the mouth of the inner cup. The pretreatment liquid is filled into the inner cup when it leaves the factory, so that there is no need to manually drip the digestion liquid before centrifugation, so as to improve the detection efficiency. At the same time, the subject adds the specimen into the sampling cup, and the cup cover can be tightened on the sampling cup. In this process, the detection personnel do not directly contact the specimen, thereby improving the protection of the detection personnel.

[0008] Furthermore, in order to facilitate the production of the sampling cup and the inner cup, the filling of the pretreatment liquid and the sealing of the inner cup, the sampling cup and the inner cup are separately arranged, so that the sampling cup and the inner cup can be produced separately, and it is convenient to fill the digestive fluid into the inner cup and seal it.

[0009] Furthermore, a fastening mechanism for fastening the inner cup is provided on the inner side of the sampling cup to ensure that the inner cup will not fall off the sampling cup during centrifugation.

[0010] Furthermore, a soft rubber pad is provided at the contact point between the cup mouth of the sampling cup and the slide, and the soft rubber pad with a certain elasticity ensures the sealing of the sampling cup, so that the specimen in the sampling cup will not be thrown out during the centrifugation process.

[0011] Furthermore, at least one side of the upper end of the base is provided with a limiting portion extending upward to ensure that the substrate is aligned with the cup opening of the sampling cup.

[0012] Beneficial effects of the present invention: According to the test items, the subject adds the specimen into the sampling cup. For example, when testing tuberculosis, the subject spits sputum into the sampling cup, and the tester adds pre-treatment liquid such as digestive fluid into the sampling cup according to the test items. During centrifugation, the tester places the sampling cup in a centrifugal device for centrifugation. During the centrifugation process, the substrate concentrates the specimen, so that after the test is completed, it is only necessary to loosen the cup cover and take out the slide, and then subsequent staining, microscopic examination and other operations can be carried out to improve the test efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a side view structural schematic diagram of Example 1.

[0014] Figure 2 This is a schematic cross-sectional view of the structure of Example 1.

[0015] Figure 3 This is a schematic diagram of the side structure of Example 2.

[0016] Figure 4 This is a schematic cross-sectional view of the second embodiment.

[0017] Figure 5 It is a schematic diagram of the structure of the present invention placed in a tooling.

[0018] Figure 6 It is a schematic diagram of the tooling structure.

[0019] The text labels in the figure are as follows: 1. sampling cup; 2. cup cover; 3. inner cup; 4. sealing cover; 5. cover body; 6. base; 7. carrier; 8. substrate; 9. scale; 10. soft rubber pad; 11. limit part; 12. tooling. DETAILED DESCRIPTION

[0020] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is described in detail below in conjunction with the accompanying drawings. The description in this part is only exemplary and explanatory and should not have any limiting effect on the protection scope of the present invention.

[0021] Embodiment 1, as Figure 1 , Figure 2 , Figure 5 , Figure 6 As shown, the structure of this embodiment is: a specimen collection device suitable for centrifugal bacteria collection method, which includes a sampling cup 1, the sampling cup 1 is preferably made of transparent materials such as glass and polycarbonate, and a scale 9 is arranged on the cup body of the sampling cup 1 along its axial direction. The cup mouth of the sampling cup 1 is equipped with a lockable cup cover 2, and the cup cover 2 can be locked on the sampling cup 1 by means of threads or the like.

[0022] The cup cover 2 includes a cover body 5 connected to the sampling cup 1 and a base 6, that is, the cover body 5 is connected to the cup body of the sampling cup 1 by threads, the cover body 5 is connected to the base 6 by a connecting section, a through hole for a slide 7 to pass through is provided between the cover body 5 and the base 6, the through hole can be provided by opening a hole on the connecting section, the slide 7 can use an existing glass slide, etc., a substrate 8 for collecting bacteria and cells is provided on the slide 7, the substrate 8 is fixed on the slide 7 by gluing, snapping and hot melting, etc., the substrate 8 is an adsorption film used in the existing medical detection field, etc. At least one side of the upper end of the base 6 is provided with a limit portion 11 extending upward, when the slide 7 is placed on the base 6, the side wall of the slide 7 needs to support the limit portion 11 on the side where it is located, and a soft rubber pad 10 is provided at the mouth of the sampling cup 1, the soft rubber pad 10 is a ring structure adapted to the mouth of the sampling cup 1, and the soft rubber pad 10 can be made of a material with a certain elasticity such as silicone and rubber.

[0023] Specific usage method: The examinee first removes the cup cover 2 from the sampling cup 1, and then spits sputum into the sampling cup 1, with the mouth of the sampling cup 1 facing upward. Then, the examinee hands the sampling cup 1 to the inspector, who adds digestion fluid, inactivation fluid, etc. into the sampling cup 1. Then the inspector screws the cup cover 2 onto the sampling cup 1 from top to bottom, so that the soft rubber pad 10 is in close contact with the slide 7, and the substrate 8 is located inside the soft rubber pad 10.

[0024] The inspector checks whether the sampling cup 1 is tightly closed, and then marks and collects it. The inspector takes the collected sampling cup 1 back to the testing center and centrifuges it in the centrifuge equipment of the testing center. Before centrifugation, the inspector places the sampling cup 1 with the cup mouth facing down on the tooling 12, which is provided with a groove that matches the base 6, and then places the tooling 12 on the centrifuge equipment, which can be a horizontal centrifuge. During the centrifugation process, bacteria, cells, etc. in the specimen are adsorbed by the substrate 8. After the centrifugation is completed, the inspector loosens the cup cover 2, takes out the slide 7 and collects it.

[0025] Embodiment 2, as Figure 3-Figure 4As shown, other structures and experimental methods of this embodiment are the same as those of Embodiment 1, but in this embodiment, an inner cup 3 is provided in the sampling cup 1, and the inner cup 3 is pre-filled with a pretreatment liquid configured according to different detection items, such as specimen digestion liquid, potassium chloride, sodium hypochlorite, etc. The inner cup 3 and the sampling cup 1 can be integrally provided. In order to prevent the pretreatment liquid from undergoing chemical reactions under light and causing deterioration or failure, the inner cup 3 is preferably made of black or other dark materials; at the same time, the dark inner cup 3 will not interfere with the observation of the specimen status in the sampling cup 1; therefore, the sampling cup 1 and the inner cup 3 are separately provided.

[0026] The inner side of the sampling cup 1 is provided with a fastening mechanism for fastening the inner cup 3. The fastening mechanism may be glue applied to the outer bottom of the inner cup 3 or the inner bottom of the sampling cup 1, and the inner cup 3 is bonded to the sampling cup 1 by the glue; or the fastening mechanism may be ribs arranged on the inner side of the side wall of the sampling cup 1, and the ribs are arranged in a circular array around the axis of the sampling cup 1, and the inner cup 3 is clamped by the ribs; or the fastening mechanism may adopt the combined effect of ribs and glue, or adopt a structure in the prior art that can fix one cup body to the inner side of another cup body.

[0027] The inner cup 3 is provided with a sealing cover 4 which can be opened automatically during centrifugation. The sealing cover 4 can be made of a film made of polyvinyl alcohol, polypropylene or other materials, and is bonded to the inner cup 3 by curing glue or the like.

[0028] Specific usage method: The examinee first removes the cup cover 2 from the sampling cup 1, and then spits sputum into the sampling cup 1, with the mouth of the sampling cup 1 facing upward. Then, the examinee screws the cup cover 2 onto the sampling cup 1 from top to bottom, so that the soft rubber pad 10 is in close contact with the slide 7, and the substrate 8 is located inside the soft rubber pad 10. After completing the above actions, the examinee hands the sampling cup 1 to the inspector.

[0029] The inspector checks whether the sampling cup 1 is tightly closed, and then marks and collects it. The inspector takes the collected sampling cup 1 back to the testing center and centrifuges it in the centrifuge equipment of the testing center. Before centrifugation, the inspector places the sampling cup 1 with the cup mouth facing down on the tooling 12, which is provided with a groove that matches the base 6, and then places the tooling 12 on the centrifuge equipment, which can be a horizontal centrifuge. During the centrifugation process, the pretreatment liquid in the inner cup 3 pushes open the sealing cover 4 under the action of centrifugal force, etc., so as to process the specimen. At the same time, bacteria and the like in the specimen are adsorbed by the substrate 8.

[0030] After the centrifugation is completed, the inspector loosens the cup cover 2 and takes out the slide 7 for collection.

[0031] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0032] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. The above is only a preferred implementation of the present invention. It should be pointed out that due to the limitations of textual expression, there are objectively infinite specific structures. For ordinary technicians in this technical field, without departing from the principles of the present invention, they can make several improvements, modifications or changes, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the protection scope of the present invention.

Claims

1. A specimen collection device suitable for a centrifugal bacteria collection method, characterized in that: The invention comprises a sampling cup (1), wherein a cup cover (2) is arranged at the cup mouth of the sampling cup (1); the cup cover (2) comprises a cover body (5) connected to the sampling cup (1) and a base (6); the cover body (5) is connected to the base (6) via a connecting section; a through hole for a slide (7) to pass through is arranged between the cover body (5) and the base (6); and a substrate (8) for collecting bacteria and cells is arranged on the slide (7).

2. A specimen collection device suitable for a centrifugal bacteria collection method according to claim 1, characterized in that: The sampling cup (1) is made of transparent material.

3. A specimen collection device suitable for centrifugal bacteria collection method according to claim 2, characterized in that: A scale (9) is arranged on the cup body of the sampling cup (1) along its axial direction.

4. A specimen collection device suitable for centrifugal bacteria collection method according to claim 1, characterized in that: An inner cup (3) is arranged inside the sampling cup (1), a pretreatment liquid is stored in the inner cup (3), and a sealing cover (4) which can be automatically opened during centrifugation is arranged at the cup mouth of the inner cup (3).

5. A specimen collection device suitable for centrifugal bacteria collection method according to claim 4, characterized in that: The sampling cup (1) and the inner cup (3) are arranged separately.

6. A specimen collection device suitable for centrifugal bacteria collection method according to claim 5, characterized in that: A fastening mechanism for fastening the inner cup (3) is provided on the inner side of the sampling cup (1).

7. A specimen collection device suitable for a centrifugal bacteria collection method according to any one of claims 1 to 6, characterized in that: A soft rubber pad (10) is provided at the contact point between the mouth of the sampling cup (1) and the slide (7).

8. A specimen collection device suitable for a centrifugal bacteria collection method according to any one of claims 1 to 6, characterized in that: At least one side of the upper end of the base (6) is provided with a limiting portion (11) extending upward.