Virtual boundary optical flow control system based on photoinduced biological micro-motor and use method of virtual boundary optical flow control system

Abstract

The invention belongs to the technical field of optical tweezers, and particularly relates to a virtual boundary optical flow control system based on a photoinduced biological micromotor and a use method of the virtual boundary optical flow control system based on the photoinduced biological micromotor. The photoinduced biological micro-motor module utilizes a micro-channel formed by chlamydomonas reinhardtii to directionally transport particles; the holographic optical tweezers module comprises a continuous laser, an optical lens, a spatial light modulator, a high NA objective lens, a dichroscope, a lighting source, a sample table and a displacement table; the micro-fluidic module comprises a micro-channel layer and a glass substrate group. Holographic optical tweezers are used for capturing chlamydomonas reinhardtii and enabling the chlamydomonas reinhardtii to rotate around a focus, swing of flagellum of the chlamydomonas reinhardtii can generate a stable flow field in surrounding fluid, a photoinduced biological micromotor is used as a driving source, and a virtual boundary is created to guide and control a flowing path of microfluid and a movement track of microparticles. And accurate control without physical obstacles is realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of optical tweezers, and in particular relates to a virtual boundary optofluidic system based on a light-induced biological micromotor and a use method thereof. Background Art

[0002] Micromotors, as micro / nano-sized energy conversion devices, can convert various forms of energy into mechanical energy. Micromotors based on optical tweezers technology ultimately convert light energy into mechanical energy and are widely used in fields such as drug delivery and disease diagnosis.

[0003] Microfluidics is a technology that uses microchannels to accurately process or manipulate microfluids. Compared with traditional methods, microfluidics improves the controllability and uniformity of micro / nano carriers. The technology of microfluidics for particle manipulation can be traced back to the 1960s when DNA molecules were separated using microfluidic channels, and the study of the behavior of fluids in micron-scale channels began. The manipulation of particles can not only be used for the capture, transportation and programmed arrangement of particles, but also for the evaluation of the physical and chemical properties of particles and the reactions or interactions between particles. Optofluidics, which organically combines optical devices and microfluidics, has a wide range of application scenarios. For example, in the fields of medical diagnosis and life sciences, it can realize single-cell analysis, drug screening and other functions.

[0004] Optical tweezers are "invisible". They can perform non-destructive and sterile manipulation of microparticles, dynamic video tracking, and control of tiny forces. This has promoted the development of biomedicine, especially in the field of drug delivery, where optical tweezers technology has shown potential to be superior to ordinary drug delivery methods. Although optical tweezers technology has shown great application potential in many fields such as biomedicine and microparticle manipulation, optical tweezers technology based on biological micromotors still faces some limitations and problems that need to be solved. For example, the capture of tiny particles using traditional optical tweezers has the following problems: laser damage to samples, especially for some samples such as proteins, nucleic acids, and biological cells, high-power lasers can cause problems such as heat loss, thereby affecting the accuracy of experimental results; different refractive indices of samples such as proteins, nucleic acids, and biological cells result in different light pressure effects on them. Therefore, when facing complex samples, detailed optimization parameters need to be set to obtain ideal sorting effects; effective particle optical sorting usually requires complex spatial optical tweezers devices, such as laser light sources and optical lenses. This increases the manufacturing cost and technical difficulty of the equipment. The manipulation of particles is achieved by applying an external field to the particles. In addition to the light field, the applied external field also includes the acoustic field, dielectric field and other external fields. According to the physical properties of the particles, such as size and dielectric constant, the distribution of different particles is differentiated. In the microchannel, the particles move in the expected direction with different flow routes or different speeds, creating conditions for the realization of microfluidics. Acoustic field manipulation is a microfluidic technology that uses the generated sound waves to drive the particles in the fluid to be acted upon by the acoustic radiation force. Similar to optical manipulation, it uses a label-free acoustic effect. It relies on the difference in acoustic radiation force acting on particles suspended in the liquid to separate particles of different sizes or particles with different physical and mechanical properties. However, since the manipulation is completed in a closed channel, the manipulation flux is low; the equipment is complex, which increases the production cost and power consumption, and it is difficult to manipulate particles with different refractive indices. Dielectric field manipulation refers to a manipulation technology in which different types of suspended particles are subjected to dielectrophoretic forces of different sizes and directions due to different dielectric properties and polarization in a non-uniform electric field, thereby generating different motion trajectories, thereby achieving separation. The magnitude of the dielectrophoretic force is related to the dielectric constant, conductivity and angular frequency of the electric field of the particles. Dielectrophoretic manipulation of particles is based on the difference in their physical properties, and does not require surface labeling. When there are significant differences in particle size and dielectric properties, the sorting accuracy is high. However, during the dielectrophoretic manipulation process, the target particles are adsorbed on the electrodes, and the power needs to be turned off and rinsed to obtain the target particles, making the operation complicated and affecting the separation efficiency.

[0005] To this end, the present invention proposes a virtual boundary optofluidic system based on light-induced biological micromotors and a method of using the same. Summary of the invention

[0006] The purpose of the present invention is to provide a virtual boundary optofluidic system based on photoinduced biological micromotors and a method for using the system. Holographic optical tweezers are used to capture Chlamydomonas reinhardtii and rotate it around a focus. The swinging of its flagella will generate a stable flow field in the surrounding fluid. Photoinduced biological micromotors are used as driving sources to create virtual boundaries to guide and control the flow path of microfluids and the motion trajectory of microparticles, thereby achieving precise manipulation without physical obstacles.

[0007] The technical solution adopted by the present invention is as follows:

[0008] A virtual boundary optofluidic system based on a photoinduced bio-micromotor, comprising a photoinduced bio-micromotor module, a holographic optical tweezers module and a microfluidic module.

[0009] The light-induced biological micromotor module is a micromotor that uses holographic optical tweezers to capture a number of directional transport microparticles formed by Chlamydomonas reinhardtii. The light-induced biological micromotor module uses the microchannel formed by Chlamydomonas reinhardtii to directional transport microparticles.

[0010] The holographic optical tweezers module includes a continuous laser, an optical lens, a spatial light modulator, a high NA objective lens, a dichroic mirror, an illumination light source, a sample stage and a translation stage. The optical lens is provided in two groups, two in each group, and the two groups of optical lenses correspond to the continuous laser and the spatial light modulator respectively. The illumination light source illuminates the sample stage, the high NA objective lens is placed at the bottom of the sample stage, the driving end of the translation stage is connected to the sample stage, and the dichroic mirror is placed directly below the high NA objective lens.

[0011] The microfluidic module comprises a microfluidic chip, and the microfluidic chip comprises a microchannel layer and a glass substrate group.

[0012] Preferably, the translation stage is controlled by a three-dimensional piezoelectric platform.

[0013] Preferably, the dichroic mirror reflects long-wavelength laser light and transmits short-wavelength visible light.

[0014] Preferably, after being captured by the light trap, the movement of the Chlamydomonas reinhardtii will be transformed into rotation around the focus, generating a flow field.

[0015] Preferably, the virtual boundary optofluidic system further comprises a monitoring feedback module, which monitors and collects information on the motion states of the microfluids and microparticles in the photoinduced bio-micromotor module and the microfluidics module.

[0016] Preferably, the monitoring feedback module includes a reflector, a filter, a CCD imaging device and a central control computer, the reflector is arranged directly below the dichroic mirror, the filter and the CCD imaging device are arranged horizontally in sequence, and the central control computer is signal-connected to the CCD imaging device and the spatial light modulator.

[0017] A method for using a virtual boundary optofluidic system based on a light-induced bio-micromotor, the method comprising the following steps:

[0018] Step 1: Prefabricate the microfluidic module;

[0019] Step 2: The laser beam with a wavelength of 1064nm emitted by the continuous laser is expanded and collimated by two optical lenses at the bottom, and then incident on the spatial light modulator for modulation. After the six specific light beams formed after modulation pass through the telescope system composed of two optical lenses in the side chain, the light spot of the light beam is adjusted to a light spot equal to the entrance pupil size of the high NA objective lens. The modulated light beam is reflected by the dichroic mirror and focused to the microfluidic channel of the sample stage through the high NA objective lens to form a capture light trap;

[0020] Step 3: Use the rotation effect of Chlamydomonas reinhardtii captured by a light trap to generate a flow field as a biological micromotor, generate a flow field through a light trap based on a continuous laser, and use the force generated by the light field to manipulate microfluids and microparticles. By adjusting the intensity and distribution of the light field, directional and selective manipulation of microfluids and microparticles is achieved. When microfluids and microparticles enter this area, they will be affected by the combined effect of the light field and the flow field, thereby achieving precise manipulation.

[0021] Preferably, while step 2 and step 3 are being performed, the Chlamydomonas reinhardtii sample is irradiated by LED light focused by a condenser, propagates along the original path through a high NA objective lens to a dichroic mirror, and is then imaged on a CCD imaging device through a tube lens composed of a reflector and a filter; the CCD imaging device monitors and collects information on the motion state of the microfluids and microparticles in the photoinduced biological micromotor module and the microfluidic module, and transmits the monitoring information to a central control computer, which dynamically adjusts the spatial light modulator according to the monitoring information, thereby directionally and selectively manipulating and transporting the microfluids and microparticles without physical contact.

[0022] The technical effects achieved by the present invention are:

[0023] The present invention uses holographic optical tweezers to capture and manipulate Chlamydomonas reinhardtii. Nowadays, as a tool for multi-particle manipulation, holographic optical tweezers have the advantage of being able to capture multiple particles and perform real-time manipulation compared to traditional single-beam optical tweezers. Chlamydomonas reinhardtii alone is used to generate micro-vortices in a specific direction. These micro-vortices can induce local flow fields that are only targeted at specific particles. This makes it possible to capture and transport particles along a controlled trajectory using only fluid forces, thereby achieving the possibility of delivering samples including proteins, nucleic acids, and biological cells. Compared with traditional technologies, this technology combines optics and fluid mechanics. In particle capture and sorting applications, first, it has the advantages of a large range of controllable particle sizes, high manipulation accuracy, and no direct contact with the particles, avoiding mechanical losses to the particles; second, the material is selected from biological cells and has good biocompatibility; third, the flow field can manipulate any particles in batches without being restricted by parameters such as refractive index.

[0024] The present invention uses holographic optical tweezers to capture Chlamydomonas reinhardtii and make it rotate around a focus. The swinging of its flagella will generate a stable flow field in the surrounding fluid. The light-induced biological micromotor is used as a driving source to create a virtual boundary to guide and control the flow path of the microfluid and the motion trajectory of the microparticles, thereby achieving precise manipulation without physical obstacles.

[0025] In the present invention, the virtual boundary optofluidic system based on photoinduced biological micromotors has the advantages of a large range of controllable particle sizes, high control accuracy, and no need for direct contact with the particles, thus avoiding mechanical losses to the particles; the materials of the virtual boundary optofluidic system based on photoinduced biological micromotors are selected from biological cells and have good biocompatibility; the flow field can control any particles in batches, is not restricted by parameters such as refractive index, and has better applicability.

[0026] In the present invention, the motion state of the microfluids and microparticles in the photoinduced biological micromotor module and the microfluidic module can be monitored and information collected in real time through the monitoring feedback module, so that the microfluids and microparticles can be directed and selectively manipulated and transported in a direct contact state, so that the particles can be captured and transported along a controlled trajectory using only fluid force, thereby achieving the delivery of samples including proteins, nucleic acids, biological cells, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a system structure diagram of a virtual boundary optofluidic system based on a light-induced biological micromotor and a method of using the same provided in an embodiment of the present invention.

[0028] Figure 2 It is a schematic diagram of a virtual boundary formed by a photoinduced bio-micromotor in a virtual boundary optofluidic system based on a photoinduced bio-micromotor and a method of using the same provided by an embodiment of the present invention.

[0029] Figure 3It is a schematic diagram of a microfluidic module in a virtual boundary optofluidic system based on a light-induced biological micromotor and a method for using the same provided in an embodiment of the present invention.

[0030] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0031] 1. Continuous laser; 2. Optical lens; 3. Spatial light modulator; 4. Central control computer; 5. Dichroic mirror; 6. High NA objective lens; 7. Illumination light source; 8. Sample stage; 9. Translation stage; 10. Reflector; 11. Filter; 12. CCD imaging device; 13. Optical lens; 14. Optical lens; 15. Optical lens; 16. Chlamydomonas reinhardtii. DETAILED DESCRIPTION

[0032] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is specifically described below in conjunction with embodiments. It should be understood that the following text is only used to describe one or several specific embodiments of the present invention, and does not strictly limit the scope of protection of the specific claims of the present invention.

[0033] Embodiment 1

[0034] like Figure 1 as well as Figure 2 As shown, a virtual boundary optofluidic system based on a photoinduced bio-micromotor comprises a photoinduced bio-micromotor module, a holographic optical tweezers module and a microfluidic module;

[0035] The light-induced biological micromotor module is a micromotor that uses holographic optical tweezers to capture several Chlamydomonas reinhardtii-formed directional transport microparticles. The light-induced biological micromotor module uses the flow rate formed by Chlamydomonas reinhardtii to flow in a controllable local fluid to directional transport microparticles.

[0036] The holographic optical tweezers module includes a continuous laser 1, an optical lens 2, a spatial light modulator 3, a high NA objective lens 6, a dichroic mirror 5, an illumination light source 7, a sample stage 8 and a displacement stage 9. The optical lens 2 is provided with two groups, each group has two, and the two groups of optical lenses 2 correspond to the continuous laser 1 and the spatial light modulator 3 respectively. The illumination light source 7 irradiates the sample stage 8. The high NA objective lens 6 is placed at the bottom of the sample stage 8. The driving end of the displacement stage 9 is connected to the sample stage 8. The displacement stage 9 is controlled by a three-dimensional piezoelectric platform, so that micron-level motion control of the sample stage can be realized. The dichroic mirror 5 is placed directly below the high NA objective lens 6. The dichroic mirror 5 reflects long-wavelength lasers and transmits short-wavelength visible light.

[0037] The microfluidic module includes a microfluidic chip, which includes a microchannel layer and a glass substrate group; the microchannel layer is designed using AutoCAD software as follows Figure 3 The layout shown, with a length and width of 2 mm and a height of 200 μm, was prepared by photolithography. Figure 3The mold shown in the figure is manufactured by using polydimethylsiloxane replication molding technology. The prepared polydimethylsiloxane microchannel layer is treated with plasma cleaning technology and then bonded with a glass substrate group to form a microfluidic chip.

[0038] Such a virtual boundary optofluidic system based on photoinduced biological micromotors has the advantages of a large range of controllable particle sizes, high control accuracy, and no need for direct contact with the particles, thus avoiding mechanical damage to the particles; such a virtual boundary optofluidic system based on photoinduced biological micromotors has the advantages of materials selected from biological cells and good biocompatibility; the flow field can manipulate any particles in batches, is not restricted by parameters such as refractive index, and has better applicability.

[0039] Preferably, after being captured by the light trap, the movement of Chlamydomonas reinhardtii is transformed into rotation around the focus, generating a flow field, which makes it possible to capture and transport particles along a controlled trajectory using only fluid forces to achieve the delivery of samples including proteins, nucleic acids, biological cells, etc.

[0040] A method for using a virtual boundary optofluidic system based on a light-induced bio-micromotor, the method comprising the following steps:

[0041] Step 1: Prefabricate the microfluidic module;

[0042] Step 1 The specific process is as follows: photopatterning a negative photoresist microfluidic structure on a glass substrate assembly; mixing prepolymer SYLGARD184 and curing agent Dow Corning in a ratio of 10:1, degassing under vacuum for 15 minutes, and then pouring it onto a photoresist mold with a thickness of 1 mm, curing it at 65°C for 2 hours, and after curing, separating the cured polydimethylsiloxane PDMS layer from the photoresist mold, and punching two through holes to connect to the inlet and outlet, and bonding it to the glass substrate assembly after treating the surface with plasma cleaning technology to obtain a microfluidic module;

[0043] Step 2: The laser beam with a wavelength of 1064nm emitted by the continuous laser 1 is expanded and collimated by the two optical lenses 2 at the bottom, and then incident on the spatial light modulator 3 for modulation. After the six specific light beams formed after modulation pass through the telescope system composed of the two optical lenses 2 in the side chain, the light spot of the light beam is adjusted to a light spot equal to the entrance pupil size of the high NA objective lens 6. The modulated light beam is reflected by the dichroic mirror 5 and focused by the high NA objective lens 6 to the microfluidic channel of the sample stage 8 to form a capture light trap;

[0044] Step 3: Using the rotation effect of Chlamydomonas reinhardtii under the capture of light trap to generate flow field as a biological micromotor, the flow field is generated by the light trap based on continuous laser 1, and the force generated by the light field is used to manipulate microfluids and microparticles. By adjusting the intensity and distribution of the light field, the directional and selective manipulation of microfluids and microparticles is completed. When microfluids and microparticles enter this area, they will be affected by the combined action of the light field and the flow field, thereby achieving precise manipulation. Its flow field regulation effect flow field is as follows Figure 2 shown.

[0045] The present invention uses holographic optical tweezers to capture and manipulate Chlamydomonas reinhardtii. Nowadays, as a tool for multi-particle manipulation, holographic optical tweezers have the advantage of being able to capture multiple particles and perform real-time manipulation compared to traditional single-beam optical tweezers. Chlamydomonas reinhardtii alone is used to generate micro-vortices in a specific direction. These micro-vortices can induce local flow fields that are only targeted at specific particles. This makes it possible to capture and transport particles along a controlled trajectory using only fluid forces, thereby achieving the possibility of delivering samples including proteins, nucleic acids, and biological cells. Compared with traditional technologies, this technology combines optics and fluid mechanics. In particle capture and sorting applications, first, it has the advantages of a large range of controllable particle sizes, high manipulation accuracy, and no direct contact with the particles, avoiding mechanical losses to the particles; second, the material is selected from biological cells and has good biocompatibility; third, the flow field can manipulate any particles in batches without being restricted by parameters such as refractive index.

[0046] The present invention uses holographic optical tweezers to capture Chlamydomonas reinhardtii and make it rotate around a focus. The swinging of its flagella will generate a stable flow field in the surrounding fluid. The light-induced biological micromotor is used as a driving source to create a virtual boundary to guide and control the flow path of the microfluid and the motion trajectory of the microparticles, thereby achieving precise manipulation without physical obstacles.

[0047] Embodiment 2

[0048] like Figure 1 as well as Figure 2 As shown, based on the first embodiment, a virtual boundary optofluidic system based on a photoinduced biological micromotor also includes a monitoring feedback module, which monitors and collects information on the motion state of the microfluids and microparticles in the photoinduced biological micromotor module and the microfluidic module.

[0049] Preferably, the monitoring feedback module includes a reflector 10, a filter 11, a CCD imaging device 12 and a central control computer 4, the reflector 10 is arranged directly below the dichroic mirror 5, the filter 11 and the CCD imaging device 12 are arranged horizontally in sequence, and the central control computer 4 is signal-connected to the CCD imaging device 12 and the spatial light modulator 3.

[0050] On the basis of Example 1, a method for using a virtual boundary optofluidic system based on a photo-induced biological micromotor is provided. While steps 2 and 3 are being performed, the Chlamydomonas reinhardtii sample is irradiated by LED light focused by a condenser, propagates along the original path through a high NA objective lens 6 to a dichroic mirror 5, and is then imaged on a CCD imaging device 12 through a tube lens composed of a reflector 10 and a filter 11; the CCD imaging device 12 monitors and collects information on the motion state of the microfluids and microparticles in the photo-induced biological micromotor module and the microfluidic module, and transmits the monitoring information to a central control computer 4, which dynamically adjusts the spatial light modulator 3 according to the monitoring information, thereby directionally and selectively manipulating and transporting the microfluids and microparticles without physical contact.

[0051] In the present invention, the motion state of the microfluids and microparticles in the photoinduced biological micromotor module and the microfluidic module can be monitored and information collected in real time through the monitoring feedback module, so that the microfluids and microparticles can be directed and selectively manipulated and transported in a direct contact state, so that the particles can be captured and transported along a controlled trajectory using only fluid force, thereby achieving the delivery of samples including proteins, nucleic acids, biological cells, etc.

[0052] The above is only a preferred embodiment of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principles of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the art unless otherwise specified and limited.

Claims

1. A virtual boundary optofluidic system based on light-induced bio-micromotors, characterized by: Including light-induced biological micromotor module, holographic optical tweezers module and microfluidics module, The light-induced biological micromotor module is a micromotor that uses holographic optical tweezers to capture a number of directional transport microparticles formed by Chlamydomonas reinhardtii. The light-induced biological micromotor module uses the microchannel formed by Chlamydomonas reinhardtii to directional transport microparticles. The holographic optical tweezers module comprises a continuous laser (1), an optical lens (2), a spatial light modulator (3), a high NA objective lens (6), a dichroic mirror (5), an illumination light source (7), a sample stage (8) and a displacement stage (9); the optical lens (2) is provided in two groups, each group having two lenses, the two groups of optical lenses (2) respectively corresponding to the continuous laser (1) and the spatial light modulator (3); the illumination light source (7) irradiates the sample stage (8); the high NA objective lens (6) is placed at the bottom of the sample stage (8); the driving end of the displacement stage (9) is connected to the sample stage (8); and the dichroic mirror (5) is placed directly below the high NA objective lens (6); The microfluidic module comprises a microfluidic chip, and the microfluidic chip comprises a microchannel layer and a glass substrate group.

2. The virtual boundary optofluidic system based on light-induced bio-micromotors according to claim 1, characterized in that: The displacement platform (9) is controlled by a three-dimensional piezoelectric platform.

3. The virtual boundary optofluidic system based on light-induced bio-micromotors according to claim 1, characterized in that: The dichroic mirror (5) reflects the laser light with a long wavelength and transmits the visible light with a short wavelength.

4. The virtual boundary optofluidic system based on light-induced bio-micromotors according to claim 1, characterized in that: After being captured by the light trap, the movement of the Chlamydomonas reinhardtii will be transformed into rotation around the focus, generating a flow field.

5. The virtual boundary optofluidic system based on light-induced bio-micromotors according to claim 1, characterized in that: It also includes a monitoring feedback module, which monitors the motion state of the microfluids and microparticles in the photo-induced biological micromotor module and the microfluidic module and collects information.

6. The virtual boundary optofluidic system based on light-induced bio-micromotors according to claim 5, characterized in that: The monitoring feedback module comprises a reflector (10), a filter (11), a CCD imaging device (12) and a central control computer (4); the reflector (10) is arranged directly below the dichroic mirror (5); the filter (11) and the CCD imaging device (12) are arranged in sequence in a horizontal direction; and the central control computer (4) is signal-connected to the CCD imaging device (12) and the spatial light modulator (3).

7. A method for using a virtual boundary optofluidic system based on a light-induced bio-micromotor, characterized in that: The virtual boundary optical flow control system is the virtual boundary optical flow control system according to any one of claims 1 to 6, and the method of using the virtual boundary optical flow control system comprises the following steps: Step 1: Prefabricate the microfluidic module; Step 2: The continuous laser (1) emits a laser beam with a wavelength of 1064 nm, which is expanded and collimated by two optical lenses (2) at the bottom, and then incident on the spatial light modulator (3) for modulation. After the six specific light beams formed after modulation pass through the telescope system composed of two optical lenses (2) in the side chain, the light spot of the light beam is adjusted to a light spot equal to the entrance pupil size of the high NA objective lens (6). The modulated light beam is reflected by the dichroic mirror (5) and focused on the microfluidic channel of the sample stage (8) through the high NA objective lens (6), forming a capture light trap; Step 3: Using the rotation effect of Chlamydomonas reinhardtii captured by a light trap to generate a flow field as a biological micromotor, a light trap based on a continuous laser (1) is used to generate a flow field, and the force generated by the light field is used to manipulate microfluids and microparticles. By adjusting the intensity and distribution of the light field, directional and selective manipulation of microfluids and microparticles is achieved.

8. A method for using a virtual boundary optofluidic system based on a light-induced bio-micromotor according to claim 7, characterized in that: While steps 2 and 3 are being performed, the Chlamydomonas reinhardtii sample is irradiated by LED light focused by a condenser, propagates along the original path through a high NA objective lens (6) to a dichroic mirror (5), and is then imaged on a CCD imaging device (12) through a tube lens composed of a reflector (10) and a filter (11); the CCD imaging device (12) monitors and collects information on the motion state of the microfluids and microparticles in the photo-induced biological micromotor module and the microfluidic module, and transmits the monitoring information to a central control computer (4), which dynamically adjusts the spatial light modulator (3) according to the monitoring information.

Images