Folic acid content detection device and method

Abstract

The invention discloses a folic acid content detection device and method, and relates to the technical field of folic acid detection. According to the water tank, the inner side wall of the water tank body is connected with a supporting ring through a connecting rod, a shaft rod is installed on the supporting ring in a matched mode through a bearing, the end of the shaft rod is connected with a base plate, and a plurality of sets of installation arms are arranged on the peripheral side wall of the base plate; a test tube clamp is rotatably mounted on any group of mounting arms, and a test tube is clamped in the test tube clamp in a matched manner. When the device is used, a test tube is placed in the test tube clamp to be clamped and fixed, then the base plate is controlled to rotate at a variable speed, different centrifugal forces are caused by different rotating speeds of the base plate, and the test tube is controlled to oscillate in the rotating process of the base plate; meanwhile, when the water tank is used, warm water at the temperature of 50 DEG C, which submerges the bottom of the test tube, is kept to be filled in the water tank body in a shaking and shaking process, so that warm water bath is carried out while shaking and shaking are carried out.

Description

Technical Field

[0001] The present invention belongs to the technical field of folic acid detection, and in particular relates to a folic acid content detection device and method. Background Art

[0002] Folic acid is a derivative of pteridine. It was originally separated from the liver. Later, it was found that the content of folic acid in the green leaves of plants is very rich, so it is named folic acid. Folic acid, also known as vitamin B9, is a water-soluble vitamin that promotes the maturation of young cells in the bone marrow. It is widely present in meat, fresh fruits and vegetables. It is a yellow crystalline powder, tasteless and odorless, insoluble in alcohol, ether and other organic solvents.

[0003] Common methods for determining folic acid content in food include: microbiological method, fluorescence spectrophotometry, high performance liquid chromatography and ultra-high performance liquid chromatography-mass spectrometry.

[0004] When testing the folic acid content, a buffer solution and an ammonia solution are first added to a mixing tank containing the sample to be tested in sequence for mixing, and the buffer solution and the ammonia solution are quantitatively added through a handheld container. This method cannot control multiple containers for oscillation and mixing at the same time when in use, and the handheld method cannot perform oscillation and mixing while performing a warm water bath. This results in cumbersome manual operations by personnel during the folic acid content testing process, which increases the workload of personnel. Summary of the invention

[0005] The purpose of the present invention is to provide a folic acid content detection device and method. When in use, a test tube is placed in a test tube clamp for clamping and fixing, and then a base plate is controlled to rotate at a variable speed. Different centrifugal forces are generated by different rotation speeds of the base plate, so that the test tube is controlled to oscillate during the rotation of the base plate. During the oscillating and shaking process, a water tank body is filled with 50°C warm water, thereby solving the problems raised by the existing background technology.

[0006] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0007] The present invention is a folic acid content detection device, comprising a water tank, the water tank comprising a water tank body with an open top, the inner side wall of the water tank body is connected to a support ring through a connecting rod, the support ring is mounted with a shaft rod through a bearing, the end of the shaft rod is connected to a base plate, and the outer peripheral side wall of the base plate is provided with a plurality of groups of mounting arms; a test tube clamp can be rotatably mounted on any group of the mounting arms, and a test tube is clamped in the test tube clamp; a gear is mounted on the shaft rod, and a crossbeam is arranged on the opposite side walls of the water tank body located on one side of the shaft rod, a rectangular groove is arranged on one side of the crossbeam, and a guide slide bar A is arranged on at least one inner side wall of the rectangular groove along its length direction; a rectangular plate is arranged in the rectangular groove, and an opening C for the rectangular plate to pass through is arranged on one side of the water tank body; a guide slide groove A cooperating with the guide slide bar A is arranged on the rectangular plate, and teeth meshing with the gear are arranged on one side of the rectangular plate.

[0008] Furthermore, it also includes a top cover arranged on the top of the sink, the top cover includes a rectangular frame, one side of the rectangular frame is provided with four L-shaped protrusions respectively resting on the corner sides of the inner wall of the sink body; the other side of the rectangular frame is provided with a transparent plate, and a handle is fixed on the upper surface of the transparent plate.

[0009] Furthermore, the test tube clamp includes a rectangular ring, and a rotating shaft is respectively installed on the two opposite outer side walls of the rectangular ring, and the opposite inner side walls of the two mounting arms are respectively provided with an axial hole matching the rotating shaft; two telescopic structures A or two telescopic structures B are respectively provided on the two opposite inner side walls of the rectangular ring, and the ends of the two telescopic structures A are connected to an arc-shaped splint A, and the ends of the two telescopic structures B are connected to an arc-shaped splint B.

[0010] Furthermore, a control structure is fixed on an outer wall of the rectangular ring, and the control structure is connected with the telescopic structure B through a channel arranged in the rectangular ring; the control structure includes two fixed blocks arranged on the outer wall of the rectangular ring, and the tube body is connected between the two fixed blocks; a convex ring is arranged on the middle inner wall of the tube body, and an exhaust hole is arranged on the convex ring, and partitions are respectively arranged on the inner walls of the tube body on both sides of the convex ring A, and an opening A is arranged on the partition, and a ceramic rod that seals with the opening A is penetrated at the opening A, one end of the ceramic rod is connected to a piston that slides along the inner wall of the tube body, and the other end is connected to an operating column that slides along the inner wall of the tube body; inlet and outlet holes are opened on the side wall of the tube body on one side of the partition, and the inlet and outlet holes are connected to the channel arranged in the rectangular ring.

[0011] Furthermore, the telescopic structure B includes an inner sleeve A and an outer sleeve A that are sealingly fitted to each other, and a spring A is connected between the inner sleeve A and the outer sleeve A; the bottom side of the outer sleeve A is provided with an opening B that is connected to the channel; the telescopic structure A includes an inner sleeve B and an outer sleeve B that are sealingly fitted to each other, and a spring B is connected between the inner sleeve B and the outer sleeve B.

[0012] Furthermore, a mounting seat is provided on the inner side wall of the water tank body, and a telescopic cylinder that can be extended and retracted in the horizontal direction is connected to the mounting seat, and an arc-shaped plate is provided at the end of the telescopic cylinder, and a cannula is provided on the upper surface of the arc-shaped plate, and a through hole is provided on the arc-shaped plate located in the cannula; when in use, a sample adding mechanism storing a solution to be added is inserted into the cannula, and then the sample adding mechanism is used to slowly drip the solution to be added into the test tube; the sample adding mechanism includes a cylinder body made of a transparent material and with scales on the outer side wall, an opening D is provided on the bottom side surface of the cylinder body, a discharge pipe passing through the through hole is connected to the outer bottom side surface of the cylinder body located at the opening D, and a plurality of limit columns are provided on the peripheral side of the inner bottom side surface of the cylinder body located at the opening D, and a blocking ball for blocking the opening D is provided in the limit column; a top plate is provided on the top of the cylinder body, an opening E is provided on the top plate, a damping layer is provided on the inner wall of the opening E, an operating rod is provided in a sealing manner in the opening E, and the operating rod is connected to the blocking ball through a traction rope; the cylinder body is connected with an air extraction hose having a spring clip.

[0013] Furthermore, a first positioning mechanism is provided on the upper surface of the substrate, and a second positioning mechanism cooperating with the first positioning mechanism is provided on the connecting rod; the first positioning mechanism includes a convex block fixed on the upper surface of the substrate, inclined surfaces are formed on both sides of the upper surface of the convex block, a plane is formed between the two inclined surfaces, and the upper surface of the plane is recessed downward to form a slot; the second positioning mechanism includes a convex tube arranged on the connecting rod, a limiting ring protruding inward is provided at the bottom end of the convex tube, and the limiting ring is connected to a movable column sliding along the inner wall of the convex tube through a spring C, and a guide groove B and a guide slide bar B cooperating with each other are provided on the outer wall of the movable column and the inner wall of the convex tube; a threaded hole is provided through the movable column along its axial direction, and a threaded column is threadedly connected at the threaded hole, and a spherical structure cooperating with the inclined surface and insertable into the slot is provided at one end of the threaded column, and a limiting plate is provided at the other end of the threaded column, and an operating block is provided on the limiting plate.

[0014] Furthermore, the bottom side surface of the slot is connected to a movable seat via a spring F, and a spherical groove matching the spherical structure is provided on the upper surface of the movable seat; a guide sleeve is provided on the bottom side surface of the movable seat, and a guide column inserted into the guide sleeve is provided on the bottom side surface of the slot; the bottom side surface of the movable seat located in the guide sleeve is connected to a movable plate with a spring K, and a pressure sensor is provided on the bottom side surface of the movable plate.

[0015] Furthermore, the bottom side of the water tank body is connected to an insulated water tank through a first connecting pipe with a valve A, a pump is provided in the insulated water tank, and the output end of the pump is connected to the bottom side of the water tank body through a second connecting pipe; and a temperature control system including a temperature sensor and a heater is provided in the insulated water tank.

[0016] A method for detecting folic acid content comprises the following steps:

[0017] Step 1: Grind the sample to be tested, weigh it accurately and put it into a test tube;

[0018] Step 2, adding a buffer solution of pH=8.0 into the test tube of step 1;

[0019] Step 3: Clamp the test tube in step 2 with the test tube clamp, and inject an appropriate amount of 50°C water into the water tank body;

[0020] Step 4, inject 2% ammonia solution into the sample adding mechanism, then place the sample adding mechanism into the cannula, open the plugging ball that blocks the opening D, and inject the ammonia solution in the sample adding mechanism into the test tube;

[0021] Step 5, controlling the rectangular plate to move at a variable speed along the length direction of the rectangular groove, and controlling the test tube to mix evenly under warm water bath conditions.

[0022] The present invention has the following beneficial effects:

[0023] When the present invention is in use, the test tube is placed in the test tube clamp for clamping and fixing, and then the base plate is controlled to rotate at variable speeds. Different centrifugal forces are generated by different rotation speeds of the base plate, so as to control the test tube to oscillate during the rotation of the base plate. At the same time, during the oscillation and shaking process, the water tank body is kept filled with 50°C warm water that submerges the bottom of the test tube, that is, a warm water bath is performed while the oscillation and shaking are performed. When in use, the detection device provided by the present invention is not only suitable for stirring and mixing folic acid, but also suitable for other operations that require oscillation and mixing in a water bath or without a water bath.

[0024] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0026] Figure 1 It is a schematic diagram of the structure of the detection device of the present invention;

[0027] Figure 2 This is a schematic diagram of the top cover structure of the present invention;

[0028] Figure 3 It is a schematic diagram of the water tank structure of the present invention;

[0029] Figure 4 for Figure 3 A partial enlarged view of the middle part;

[0030] Figure 5 for Figure 3 A top view of

[0031] Figure 6 It is a schematic diagram of the structure of the first positioning mechanism of the present invention;

[0032] Figure 7 It is a schematic diagram of the structure of the second positioning mechanism of the present invention;

[0033] Figure 8 It is a schematic diagram of the structure of the slot of the present invention;

[0034] Fig. 9 It is a schematic diagram of the structure of the sample adding mechanism of the present invention;

[0035] Fig.10 This is a schematic diagram of the matching structure of the test tube clamp and the test tube of the present invention;

[0036] Fig.11 for Fig.10 A top view of

[0037] Fig.12 It is a schematic diagram of the control structure of the present invention;

[0038] Fig.13 It is a schematic diagram of the matching structure of the heat preservation water tank and the water tank of the present invention. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] In the description of the present invention, it should be understood that the terms "opening A", "upper", "lower", "thickness", "top", "middle", "length", "inside", "all around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0041] See also Figure 3-5 As shown, the present invention is a folic acid content detection device, including a water tank 1a, the water tank 1a includes a water tank body 1 with an open top; and the inner side wall of the water tank body 1 is connected to a support ring through a connecting rod 10, and a shaft 11 is installed on the support ring through a bearing, and the end of the shaft 11 is connected to a base plate 12, and twelve groups of mounting arms 121 are arranged on the outer peripheral side wall of the base plate 12; any group of mounting arms 121 can be rotatably mounted with a test tube clamp 2, so that when in use, the test tube 100 is placed in the test tube clamp 2 for clamping and fixing, and then the base plate 12 is controlled to rotate at a variable speed, and different centrifugal forces are caused by different rotation speeds of the base plate 12, so that the test tube 100 is controlled to oscillate during the rotation of the base plate 12.

[0042] In order to facilitate the driving substrate 12 to rotate at a variable speed according to the needs when in use, a gear 111 is installed on the shaft 11 located above the support ring, and a crossbeam 13 is provided on the two opposite side walls of the water tank body 1 located on one side of the shaft 11, and a rectangular groove 131 is provided on one side of the crossbeam 13, and a guide slide A is provided on at least one inner side wall of the rectangular groove 131 along its length direction; a rectangular plate 132 is provided in the rectangular groove 131, and a guide slide A is provided on one side of the water tank body 1 for the rectangular plate 132 to pass through. The rectangular plate 132 is provided with a guide groove A133 cooperating with the guide slide bar A, and one side of the rectangular plate 132 is provided with a tooth 135 meshing with the gear 111, that is, when in use, the rectangular plate 132 can be operated by controlling one end of the rectangular plate 132 to perform plugging and unplugging movements along the length direction of the rectangular groove 131, and the plugging and unplugging movement with variable speed can be performed when in use, and then the teeth 135 are meshed with the gear 111 to drive the base plate 12 to rotate with variable speed according to needs when in use.

[0043] It should be noted that during use, the water tank body 1 is filled with 50°C warm water that covers the bottom of the test tube 100 during the shaking process, that is, a warm water bath is performed while the shaking process is performed; in order to achieve this function, Fig.13 A first connecting pipe 61 with a valve A is connected to an insulated water tank 6 on the bottom side of the water tank body 1, a pump 63 is arranged in the insulated water tank 6, and the output end of the pump 63 is connected to the bottom side of the water tank body 1 through a second connecting pipe 62; and a temperature control system including a temperature sensor and a heater is arranged in the insulated water tank 6; based on the above arrangement, through the arrangement of the insulated water tank 6, the water level in the water tank body 1 can be controlled to change according to demand during use, and the water temperature of the water bath can be kept at a constant temperature of about 50°C during long-term use.

[0044] At the same time, in order to avoid excessive heat dissipation caused by the top of the water tank body 1 being opened during the shaking process, Figure 1-2 A top cover 1b is arranged on the top of the water tank 1a, and the top cover 1b includes a rectangular frame 32. Four L-shaped protrusions 33 are arranged on one side of the rectangular frame 32, which are respectively against the corner sides of the inner wall of the water tank body 1; a transparent plate 30 is arranged on the other side of the rectangular frame 32, and a handle 31 is fixed on the upper surface of the transparent plate 30. The arrangement of the transparent plate 30 can not only form a heat preservation effect when in use, but also facilitate observation of the internal situation of the water tank body 1.

[0045] Specifically, in a possible implementation manner, in order to facilitate the use of the test tube clamp 2 to clamp and fix the test tube 100, and to control the test tube 100 to be pulled out of the test tube clamp 2 during use, as shown in FIG. Figure 10-11 The test tube clamp 2 mentioned in this scheme includes a rectangular ring 20, on the two opposite outer side walls of the rectangular ring 20, a rotating shaft 21 is respectively installed, and the opposite inner side walls of the two mounting arms 121 are respectively provided with shaft holes matched with the rotating shaft 21; on the two opposite inner side walls of the rectangular ring 20, two telescopic structures A22 or two telescopic structures B27 are respectively provided, and the ends of the two telescopic structures A22 are connected to an arc-shaped clamping plate A23, and the ends of the two telescopic structures B27 are connected to an arc-shaped clamping plate B28, and a control structure is fixed on an outer side wall of the rectangular ring 20, and the control structure is connected to the telescopic structure B27 through a channel provided in the rectangular ring 20; Fig.12 The control structure includes two fixed blocks 24 arranged on the outer wall of the rectangular ring 20, and the tube body 26 is connected between the two fixed blocks 24; a convex ring 261 is arranged on the inner wall of the middle part of the tube body 26, and an exhaust hole 262 is arranged on the convex ring 261, and partitions 264 are respectively arranged on the inner walls of the tube body 26 located on both sides of the convex ring A261, and an opening A265 is arranged on the partition 264. A ceramic rod 252 that seals with the opening A265 is penetrated through the opening A265, one end of the ceramic rod 252 is connected to a piston 253 that slides along the inner wall of the tube body 26, and the other end is connected to an operating column 25 that slides along the inner wall of the tube body 26; an air inlet and outlet hole 263 is opened on the side wall of the tube body 26 located on one side of the partition 264, and the air inlet and outlet hole 263 is connected to the channel arranged in the rectangular ring 20.

[0046] Based on the settings of the above-mentioned control structure, telescopic structure A22 and telescopic structure B27, it is realized that during use, when the operating column 25 of the control structure slides inward along the inner wall of the tube body 26, under the action of air pressure, the arc splint B28 moves toward the side away from the arc splint A23, that is, the clamping space formed by the arc splint B28 and the arc splint A23 is larger. At this time, the test tube 100 can be inserted from top to bottom into the formed clamping space, and the external force of the control operating column 25 is removed. At this time, the telescopic structure B27 extends out under the action of itself, and then the arc splint B28 and the arc splint A23 cooperate to clamp and fix the test tube 100 inserted between the two.

[0047] Specifically, in order to improve the fixing effect of the test tube 100 and facilitate the subsequent removal of the test tube 100, the telescopic structure B27 includes an inner sleeve A and an outer sleeve A that are sealed and socketed with each other, and a spring A is connected between the inner sleeve A and the outer sleeve A; the bottom side of the outer sleeve A is provided with an opening B that is connected to the channel; the telescopic structure A22 includes an inner sleeve B and an outer sleeve B that are socketed with each other, and a spring B is connected between the inner sleeve B and the outer sleeve B.

[0048] In the process of determining the folic acid content, in order to improve the mixing and heating effect of the solutions in the same batch of test tubes 100, auxiliary reagents need to be added to the test tubes 100 at approximately the same time, such as Figure 5 , and then the present invention sets a mounting seat 15 on the inner side wall of the water tank body 1, and a telescopic cylinder 151 that is telescopic in the horizontal direction is connected to the mounting seat 15, and an arc plate 152 is set at the end of the telescopic cylinder 151, and a cannula 16 is set on the upper surface of the arc plate 152, and a through hole 161 is set on the arc plate 152 located in the cannula 16; when in use, the sample adding mechanism storing the solution to be added is inserted into the cannula 16, and then the sample adding mechanism is used to slowly drop the solution to be added into the test tube 100; Fig. 9 The sample loading mechanism includes a cylinder 4 made of a transparent material and with scales set on the outer wall. An opening D40 is set on the bottom side of the cylinder 4. The outer bottom side of the cylinder 4 located at the opening D40 is connected to a discharge pipe 41 passing through the through hole 161. A plurality of limiting columns 42 are set around the inner bottom side of the cylinder 4 located at the opening D40. A blocking ball 43 for blocking the opening D40 is arranged in the limiting columns 42; a top plate 44 is arranged on the top of the cylinder 4, an opening E46 is arranged on the top plate 44, a damping layer is arranged on the inner wall of the opening E46, an operating rod 48 is sealed and arranged in the opening E46, and the operating rod 48 is connected to the blocking ball 43 through a traction rope 47; the cylinder 4 is connected with an exhaust hose 49 with a spring clip 45.

[0049] The present invention also provides a method for detecting folic acid content, comprising the following steps:

[0050] Step 1: Grind the sample to be tested, weigh it accurately and put it into a test tube 100;

[0051] Step 2, adding a buffer solution with pH=8.0 into the test tube 100 of step 1;

[0052] Step 3, clamp the test tube 100 in step 2 at the test tube clamp 2, and inject an appropriate amount of 50°C water into the water tank body 1;

[0053] Step 4, inject 2% ammonia solution into the sample adding mechanism, then place the sample adding mechanism into the cannula 16, open the plugging ball 43 that blocks the opening D40, and inject the ammonia solution in the sample adding mechanism into the test tube 100;

[0054] Step 5, controlling the rectangular plate 132 to move at a variable speed along the length direction of the rectangular groove 131, and controlling the test tube 100 to mix in a warm water bath to obtain a sample solution to be tested;

[0055] Step 6, inject the folic acid standard series working solutions into the high performance liquid chromatograph respectively, determine the corresponding chromatographic peak area, draw a standard curve with the concentration of the standard working solution as the abscissa and the peak area as the ordinate; inject the sample solution to be tested into the high performance liquid chromatograph, obtain the chromatographic peak area under the same chromatographic conditions, and obtain the concentration of folic acid in the test solution according to the standard curve; calculate the folic acid content in the sample to be tested according to the calculation formula.

[0056] W=(C*V) / M

[0057] W: the content of folic acid in the sample to be tested, in ug / g;

[0058] C: The concentration of folic acid calculated from the standard curve, in ug / mL;

[0059] V: volume of the sample after dilution, unit: mL; M: mass of the sample to be tested, unit: g.

[0060] The concentrations of the folic acid standard series working solutions can be 20 μg / mL, 18 μg / mL, 16 μg / mL, 14 μg / mL, 12 μg / mL, 10 μg / mL, 8 μg / mL, 6 μg / mL, 4 μg / mL, 2 μg / mL and pure water, respectively.

[0061] In the above step 3, valve B provided on the second connecting pipe 62 is opened and pump 63 is started at the same time, so that warm water at about 50°C stored in the insulated water tank 6 can be pumped into the water tank body 1; when the warm water is added, valve B and pump 63 are closed; and at the same time, after the test tube 100 is mixed in step 5, before taking out the test tube 100, valve A provided on the first connecting pipe 61 is opened first, so that the warm water stored in the water tank body 1 can flow back to the insulated water tank 6 under the action of its own gravity.

[0062] It can be known that in the above step 4, in order to facilitate the injection of 2% ammonia solution into the sample addition mechanism, the vacuum hose 49 is controlled to connect the negative pressure device and the bottom end of the discharge pipe 41 is inserted below the liquid surface of the ammonia solution. At this time, the ammonia solution can be injected into the cylinder 4. In this process, the spring clamp 45 is first opened and then closed; at the same time, the sample addition mechanism is placed in the insert tube 16 and the blocking ball 43 that blocks the opening D40 is opened. At this time, under the action of gravity, the ammonia solution in the cylinder 4 is slowly added to the test tube 100 through the discharge pipe 41; in the above, in order to facilitate the control of the blocking ball 43 to move upward from the opening D40, the operating rod 48 is used to pull upward, and damping is formed between the operating rod 48 and the damping layer, so that it is convenient to control the operating rod 48 to be pulled upward when in use, and the operating rod 48 and the damping layer remain relatively still without the action of external artificial force.

[0063] Based on the above content, it can be known that in order to conveniently control the ammonia solution in the sample adding mechanism to be discharged into the test tube 100, it is necessary to keep the test tube 100 and the sample adding mechanism aligned during use. Since the substrate 12 is in rotation during use, and the alignment of the test tube 100 and the sample adding mechanism cannot be guaranteed after rotation, based on the technical requirement of alignment, the present invention is provided with a first positioning mechanism 17 on the upper surface of the substrate 12, and a second positioning mechanism cooperating with the first positioning mechanism 17 is provided on the connecting rod 10; Figure 6 The first positioning mechanism 17 includes a protrusion 17 fixed on the upper surface of the substrate 12, and the protrusion 17 has inclined surfaces 172 on both sides of the upper surface, and a plane 173 is formed between the two inclined surfaces 172. The upper surface of the plane 173 is recessed downward to form a slot 174; Figure 4 and 7The second positioning mechanism includes a convex tube 18 arranged on the connecting rod 10, and a limiting ring 181 protruding inward is arranged at the bottom end of the convex tube 18, and the limiting ring 181 is connected to a movable column 183 sliding along the inner wall of the convex tube 18 through a spring C182, and the outer wall of the movable column 183 and the inner wall of the convex tube 18 are provided with a guide groove B and a guide slide bar B that cooperate with each other; the movable column 183 is provided with a threaded hole 184 along its axial direction, and a threaded column 185 is threadedly connected at the threaded hole 184, and one end of the threaded column 185 is provided with a spherical structure 186 that cooperates with the inclined surface 172 and can be inserted into the slot 174, and the other end of the threaded column 185 has a limiting plate 187, and the limiting plate 18 7 is provided with an operation block 188; when it is necessary to control the alignment of the test tube 100 and the sample loading mechanism during use, the threaded column 185 is rotated to make it move vertically downward until the limit plate 187 abuts against the end of the movable column 183; then the control substrate 12 is rotated, and when it is rotated until the second positioning mechanism and the first positioning mechanism 17 enter the matching position, the spherical structure at the end of the threaded column 185 first contacts and abuts against the inclined surface 172, and the control substrate 12 continues to rotate, and the spring C182 contracts at this time; and then when it is rotated until the spherical structure 186 is aligned with the slot 174, the spring C182 is extended, and the spherical structure 186 is inserted into the slot 174, and the further rotation of the substrate 12 is prevented to achieve the limit positioning effect.

[0064] On the basis of the above, in order to facilitate the reminder that the second positioning mechanism and the first positioning mechanism 17 on the substrate 12 and the connecting rod 10 are in a matched positioning state, such as Figure 8 A movable seat 1742 is connected to the bottom side of the slot 174 through a spring F1741, and a spherical groove 1743 is provided on the upper surface of the movable seat 1742 to cooperate with the spherical structure 186; a guide sleeve 1745 is provided on the bottom side of the movable seat 1742, and a guide column 1744 inserted into the guide sleeve 1745 is provided on the bottom side of the slot 174; a movable plate 1748 is connected to the bottom side of the movable seat 1742 located in the guide sleeve 1745 and the spring K1747, A pressure sensor 1746 is provided on the bottom side of the movable plate 1748, and the pressure sensor 1746 is connected to a controller, and the controller is connected to an indicator light, which is provided at the top of an outer wall of the water tank body 1; that is, when the spherical structure 186 is inserted into the slot 174, the spherical structure 186 is inserted into the ball groove 1743, driving the movable seat 1742 to move downward, and the pressure sensor 1746 moves downward until it contacts the top of the guide column 1744 to generate a pressure signal, and then the control indicator light is turned on.

[0065] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0066] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A folic acid content detection device, characterized in that: The invention comprises a water tank (1a), wherein the water tank (1a) comprises a water tank body (1) with an open top, the inner side wall of the water tank body (1) is connected to a support ring via a connecting rod (10), a shaft rod (11) is mounted on the support ring via a bearing, the end of the shaft rod (11) is connected to a base plate (12), and a plurality of groups of mounting arms (121) are arranged on the outer peripheral side wall of the base plate (12); A test tube clamp (2) can be rotatably mounted on any group of the mounting arms (121), and a test tube (100) is cooperatively clamped inside the test tube clamp (2); A gear (111) is mounted on the shaft (11); a crossbeam (13) is arranged on opposite side walls of the water tank body (1) on one side of the shaft (11); a rectangular groove (131) is arranged on one side of the crossbeam (13); a guide slide bar A is arranged on at least one inner side wall of the rectangular groove (131) along its length direction; a rectangular plate (132) is arranged in the rectangular groove (131); an opening C for the rectangular plate (132) to pass through is arranged on one side of the water tank body (1); The rectangular plate (132) is provided with a guide slot A (133) matched with the guide slide bar A, and one side of the rectangular plate (132) is provided with teeth (135) meshing with the gear (111).

2. A folic acid content detection device according to claim 1, characterized in that: It also includes a top cover (1b) arranged on the top of the water tank (1a), wherein the top cover (1b) includes a rectangular frame (32), and one side of the rectangular frame (32) is provided with four L-shaped protrusions (33) respectively abutting against the corners of the inner side wall of the water tank body (1); A transparent plate (30) is disposed on the other side of the rectangular frame (32), and a handle (31) is fixed on the upper surface of the transparent plate (30).

3. A folic acid content detection device according to claim 1, characterized in that: The test tube clamp (2) comprises a rectangular ring (20), on two opposite outer side walls of the rectangular ring (20) are respectively mounted a rotating shaft (21), and on the opposite inner side walls of the two mounting arms (121) are respectively provided an axial hole matching the rotating shaft (21); Two telescopic structures A (22) or two telescopic structures B (27) are respectively arranged on two opposite inner side walls of the rectangular ring (20), the ends of the two telescopic structures A (22) are connected to an arc-shaped clamping plate A (23), and the ends of the two telescopic structures B (27) are connected to an arc-shaped clamping plate B (28).

4. A folic acid content detection device according to claim 3, characterized in that: A control structure is fixed on an outer side wall of the rectangular ring (20), and the control structure is connected to the telescopic structure B (27) through a channel arranged in the rectangular ring (20); The control structure comprises two fixing blocks (24) arranged on the outer side wall of the rectangular ring (20), and a tube body (26) is connected between the two fixing blocks (24); A convex ring (261) is provided on the inner wall of the middle part of the tube body (26), and an exhaust hole (262) is provided on the convex ring (261). Partition plates (264) are respectively provided on the inner walls of the tube body (26) located on both sides of the convex ring A (261), and an opening A (265) is provided on the partition plates (264). A ceramic rod (252) is provided through the opening A (265) and is sealed with the opening A (265). One end of the ceramic rod (252) is connected to a piston (253) that slides along the inner wall of the tube body (26), and the other end is connected to an operating column (25) that slides along the inner wall of the tube body (26); An air inlet and outlet hole (263) is provided on the side wall of the tube body (26) located on one side of the partition plate (264), and the air inlet and outlet hole (263) is connected to a channel arranged in the rectangular ring (20).

5. A folic acid content detection device according to claim 4, characterized in that: The telescopic structure B (27) comprises an inner sleeve A and an outer sleeve A which are sealed and sleeved with each other, and a spring A is connected between the inner sleeve A and the outer sleeve A; an opening B which is connected to the channel is provided on the bottom side of the outer sleeve A; The telescopic structure A (22) comprises an inner sleeve B and an outer sleeve B which are sleeved together, and a spring B is connected between the inner sleeve B and the outer sleeve B.

6. A folic acid content detection device according to claim 5, characterized in that: The inner side wall of the water tank body (1) is provided with a mounting seat (15), a telescopic cylinder (151) that is telescopic in the horizontal direction is connected to the mounting seat (15), an arc-shaped plate (152) is provided at the end of the telescopic cylinder (151), an insert pipe (16) is provided on the upper surface of the arc-shaped plate (152), and a through hole (161) is provided on the arc-shaped plate (152) located inside the insert pipe (16); When in use, the sample adding mechanism storing the solution to be added is inserted into the insertion tube (16), and then the solution to be added is slowly dripped into the test tube (100) using the sample adding mechanism; The sample loading mechanism comprises a cylinder (4) made of a transparent material and having scales on its outer wall, an opening D (40) being arranged on the bottom side of the cylinder (4), a discharge pipe (41) passing through the through hole (161) being connected to the outer bottom side of the cylinder (4) at the opening D (40), a plurality of limiting columns (42) being arranged around the inner bottom side of the cylinder (4) at the opening D (40), a blocking ball (43) for blocking the opening D (40) being arranged in cooperation with the limiting column (42); A top plate (44) is arranged on the top of the cylinder (4), an opening E (46) is arranged on the top plate (44), a damping layer is arranged on the inner wall of the opening E (46), an operating rod (48) is arranged in a sealing manner in the opening E (46), and the operating rod (48) is connected to the blocking ball (43) through a traction rope (47); the cylinder (4) is connected to an exhaust hose (49) with a spring clip (45).

7. A folic acid content detection device according to claim 6, characterized in that: A first positioning mechanism (17) is disposed on the upper surface of the base plate (12), and a second positioning mechanism cooperating with the first positioning mechanism (17) is disposed on the connecting rod (10); The first positioning mechanism (17) comprises a convex block (17) fixed on the upper surface of the substrate (12); inclined surfaces (172) are formed on both sides of the upper surface of the convex block (17); a plane (173) is formed between the two inclined surfaces (172); and the upper surface of the plane (173) is recessed downward to form a slot (174); The second positioning mechanism comprises a convex tube (18) arranged on the connecting rod (10), a limiting ring (181) protruding inward is arranged at the bottom end of the convex tube (18), the limiting ring (181) is connected to a movable column (183) sliding along the inner wall of the convex tube (18) through a spring C (182), and the outer wall of the movable column (183) and the inner wall of the convex tube (18) are provided with a guide groove B and a guide slide bar B that cooperate with each other; the movable column (183) is provided with a threaded hole (184) along its axial direction, and a threaded column (185) is threadedly connected at the threaded hole (184), one end of the threaded column (185) is provided with a spherical structure (186) that cooperates with the inclined surface (172) and can be inserted into the slot (174), and the other end of the threaded column (185) is provided with a limiting plate (187), and an operating block (188) is provided on the limiting plate (187).

8. A folic acid content detection device according to claim 7, characterized in that: The bottom side of the slot (174) is connected to a movable seat (1742) via a spring F (1741), and the upper surface of the movable seat (1742) is provided with a spherical groove (1743) that cooperates with the spherical structure (186); A guide sleeve (1745) is disposed on the bottom side of the movable seat (1742), and a guide column (1744) inserted into the guide sleeve (1745) is disposed on the bottom side of the slot (174); A movable plate (1748) is connected to the bottom side of the movable seat (1742) in the guide sleeve (1745) and the spring K (1747), and a pressure sensor (1746) is provided on the bottom side of the movable plate (1748).

9. A folic acid content detection device according to claim 8, characterized in that: The bottom side of the water tank body (1) is connected to a heat preservation water tank (6) via a first connecting pipe (61) provided with a valve A; a pump (63) is provided in the heat preservation water tank (6); the output end of the pump (63) is connected to the bottom side of the water tank body (1) via a second connecting pipe (62); and a temperature control system including a temperature sensor and a heater is provided in the heat preservation water tank (6).

10. A method for detecting folic acid content based on a folic acid content detection device as described in any one of claims 6 to 9, characterized in that: The method comprises the following steps: Step 1, grind the sample to be tested into powder, weigh it accurately and put it into a test tube (100); Step 2, adding a buffer solution with pH=8.0 into the test tube (100) of step 1; Step 3, clamp the test tube (100) in step 2 at the test tube clamp (2), and inject an appropriate amount of 50° C. water into the water tank body (1); Step 4, inject 2% ammonia solution into the sample adding mechanism, then place the sample adding mechanism into the insert tube (16), open the plugging ball (43) that blocks the opening D (40), and inject the ammonia solution in the sample adding mechanism into the test tube (100); Step 5: Control the rectangular plate (132) to move at a variable speed along the length direction of the rectangular groove (131), and control the test tube (100) to mix evenly under warm water bath conditions.

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