Sampling and detection integrated device and method for helicobacter pylori antigen detection

Abstract

The invention discloses a sampling and detection integrated device and method for helicobacter pylori antigen detection, and relates to the technical field of helicobacter pylori antigen detection. The sampling and detection integrated device for helicobacter pylori antigen detection comprises a sample collection bottle, the top of the sample collection bottle is in threaded connection with a top cover, a visual window is formed in the outer surface of the sample collection bottle, a hollow sampling rod is fixedly connected to the top of the inner surface of the top cover, and a first rubber plug is arranged at the bottom end of the inner surface of the hollow sampling rod; a connecting rod is fixedly connected to the top of the first rubber plug, a threaded part is arranged on the outer surface of the hollow sampling rod, a groove is formed in the bottom of the sample collection bottle, a sample cavity is formed in the sample collection bottle, a fixing shaft is fixedly connected to the inner surface of the groove, a connecting seat is rotatably connected to the outer surface of the fixing shaft, and a test card is arranged in the connecting seat; the device integrates sampling and detection, the size of the device is reduced, and the device is convenient to carry.

Description

Technical Field

[0001] The present invention relates to the technical field of Helicobacter pylori antigen detection, and in particular to a sampling and detection integrated device and method for Helicobacter pylori antigen detection. Background Art

[0002] Helicobacter pylori detection refers to a series of medical testing methods used to diagnose whether a person is infected with Helicobacter pylori. Helicobacter pylori is a microorganism that can survive in the stomach and is closely related to a variety of upper gastrointestinal diseases (such as peptic ulcer, gastritis, etc.) and certain extragastric diseases (such as iron deficiency anemia, idiopathic thrombocytopenic purpura, etc.). The main methods for Helicobacter pylori detection can be divided into two categories: non-invasive and invasive. Invasive detection methods include bacterial culture, immunohistochemistry, chemical staining, rapid urease test, etc. Non-invasive detection methods include urea breath test, stool antigen test, serum antibody test, etc.

[0003] In the prior art, Helicobacter pylori stool detection is usually performed by using a sample collector and a test card together, which is inconvenient to operate. Moreover, when the stool to be sampled is loose, it is difficult to collect it with the existing sample collector, and a dropper must be used for sampling, which is inconvenient. Summary of the invention

[0004] In view of the deficiencies of the existing problems, the present invention provides an integrated sampling and detection device and method for Helicobacter pylori antigen detection to solve the existing problems raised in the above background technology.

[0005] To solve the above problems, the present invention is implemented through the following technical solutions: a sampling and detection integrated device for Helicobacter pylori antigen detection, comprising a sample collection bottle, the top of the sample collection bottle is threadedly connected to a top cover, the outer surface of the sample collection bottle is provided with a visual window, the top of the inner surface of the top cover is fixedly connected to a hollow sampling rod, the bottom end of the inner surface of the hollow sampling rod is provided with a first rubber stopper, the top of the first rubber stopper is fixedly connected to a connecting rod, the outer surface of the hollow sampling rod is provided with a threaded portion, the bottom of the sample collection bottle is provided with a groove, the interior of the sample collection bottle is provided with a sample cavity, and the inner surface of the groove is fixedly connected to a fixed shaft The outer surface of the fixed shaft is rotatably connected to a connecting seat, a test card is arranged inside the connecting seat, a supporting pull rod and a card block are fixedly connected to the outer surface of the connecting seat, a card groove is opened on the outer surface of the sample collection bottle, a guide outlet is arranged at the bottom of the sample collection bottle, a right-angle catheter is fixedly connected to the inside of the visual window, a first piston and a second piston are arranged inside the right-angle catheter, a first push rod is fixedly connected to the outer surface of the first piston, a second push rod is fixedly connected to the lower surface of the second piston, a pressure block is fixedly connected to the end of the first push rod away from the first piston, and a second rubber stopper is fixedly connected to the end of the second push rod away from the second piston.

[0006] Preferably, the outer surface of the connecting rod is sleeved inside the top cover, and the top end of the connecting rod is fixedly connected with a pull ring, and the pull ring is arranged above the top cover.

[0007] Preferably, the card block and the test card are arranged on a side of the connecting seat close to the sample collection bottle, and the supporting pull rod is arranged on a side of the connecting seat away from the card block and the test card.

[0008] Preferably, a sample adding area and a display area are provided on the outer surface of the test card, and the outer surface of the card block is matched with the inner surface of the card slot.

[0009] Preferably, the right-angle tube is arranged inside the sample chamber, the first piston is arranged at the horizontal part of the right-angle tube, and the second piston is arranged at the vertical part of the right-angle tube.

[0010] Preferably, outer surfaces of the first push rod and the second push rod are both sleeved inside the right-angle conduit, and the outer surfaces of the first push rod and the second push rod respectively pass through two ends of the right-angle conduit.

[0011] Preferably, the pressing block is arranged outside the visual window, the outer surface of the second rubber stopper is squeezed and fitted with the inner surface of the outlet, and a liquid column is arranged at the interval between the first piston and the second piston.

[0012] Preferably, the recessed portion on the outer surface of the pressing block is rotatably connected to a limiting rod, the outer surface of the viewing window is fixedly connected to a first magnetic sheet, and the end of the limiting rod is fixedly connected to a second magnetic sheet.

[0013] The invention discloses a method for using an integrated sampling and testing device for detecting Helicobacter pylori antigens, comprising the following steps: when sampling, unscrewing and removing a top cover from a sample collection bottle, adding a diluent into the sample collection bottle, inserting a threaded portion on a hollow sampling rod into a stool sample, obtaining an appropriate amount of sample by using a groove on the threaded portion, and then placing the hollow sampling rod into a sample cavity; if the stool is loose stool, pulling a pull ring upward, a first rubber plug slides along the inside of the hollow sampling rod, so that the hollow sampling rod absorbs loose stool; after placing the hollow sampling rod into the sample cavity, pressing the pull ring downward, so that the loose stool is squeezed into the sample cavity, tightening the top cover and shaking well; when testing, placing the sample collection bottle on a table, and then rotating a connecting seat to a horizontal state, so that a sample addition area is just below a guide outlet, pressing a pressing block, so that a second push rod pushes a second rubber plug, the guide outlet is opened, and a sample solution in the sample cavity drips into the sample addition area; and after the test card is left to stand for fifteen minutes, the result of a display area is observed.

[0014] The present invention provides a sampling and detection integrated device and method for Helicobacter pylori antigen detection, which has the following beneficial effects:

[0015] 1. The sampling and testing integrated device for Helicobacter pylori antigen detection, through the cooperation between the sample collection bottle, the top cover, the hollow sampling rod, the connecting seat, the pressure block and the second rubber stopper, uses the hollow sampling rod to take samples, shakes the sample in the sample collection bottle, presses the pressure block to make the sample solution fall into the test card in the connecting seat, and obtains the test result. Through the cooperation of the above structures, the device integrates sampling and testing, reduces the size of the device, and is easy to carry.

[0016] 2. The integrated sampling and testing device for Helicobacter pylori antigen detection cooperates with the connecting seat, fixed axis, test card, sample loading area and display area. When the connecting seat is rotated to a horizontal state during detection, the sample loading area is just below the outlet, which facilitates the exported sample solution to fall accurately into the sample loading area of ​​the test card, thereby improving the accuracy of the detection operation and reducing errors.

[0017] 3. The integrated sampling and detection device for Helicobacter pylori antigen detection can collect not only normal feces but also loose feces through the coordination between the hollow sampling rod, the first rubber stopper, the connecting rod and the pull ring, thereby improving the practicability of the device.

[0018] 4. The integrated sampling and detection device for Helicobacter pylori antigen detection, through the cooperation between the pressing block, the second rubber stopper, the right-angle catheter, the first piston, the second piston, the first push rod and the second push rod, only needs to press the pressing block when the sample solution needs to be exported, and no additional operating steps are required. The operation is simple and convenient, which further improves the convenience of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 A second perspective structural schematic diagram of the present invention as a whole;

[0021] Figure 3 It is a schematic diagram of the structure of the present invention during overall detection;

[0022] Figure 4 It is a schematic cross-sectional structure diagram of the present invention as a whole;

[0023] Figure 5 It is a schematic diagram of the structure of a part of the present invention;

[0024] Figure 6 It is a schematic structural diagram of the top cover and the sampling rod of the present invention.

[0025] In the figure: 1. sample collection bottle; 2. top cover; 3. visual window; 4. hollow sampling rod; 5. first rubber stopper; 6. connecting rod; 7. pull ring; 8. groove; 9. fixed axis; 10. connecting seat; 11. test card; 12. sample adding area; 13. display area; 14. supporting pull rod; 15. card block; 16. card slot; 17. guide outlet; 18. right-angle catheter; 19. first push rod; 20. second push rod; 21. liquid column; 22. pressure block; 23. second rubber stopper; 24. first magnetic sheet; 25. limit rod; 26. second magnetic sheet; 27. threaded part; 28. sample chamber; 29. ​​first piston; 30. second piston. DETAILED DESCRIPTION

[0026] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

[0027] Example 1

[0028] like Figure 1-Figure 6 As shown, the present invention provides a technical solution: a sampling and detection integrated device for Helicobacter pylori antigen detection, comprising a sample collection bottle 1, a top cover 2 is threadedly connected to the top of the sample collection bottle 1, a visual window 3 is arranged on the outer surface of the sample collection bottle 1, a hollow sampling rod 4 is fixedly connected to the top of the inner surface of the top cover 2, a first rubber stopper 5 is arranged at the bottom end of the inner surface of the hollow sampling rod 4, a connecting rod 6 is fixedly connected to the top of the first rubber stopper 5, a threaded portion 27 is arranged on the outer surface of the hollow sampling rod 4, a groove 8 is arranged at the bottom of the sample collection bottle 1, a sample cavity 28 is arranged inside the sample collection bottle 1, a fixed shaft 9 is fixedly connected to the inner surface of the groove 8, and a connecting rod 6 is rotatably connected to the outer surface of the fixed shaft 9. The base 10 includes a test card 11 disposed inside the connecting base 10, a support rod 14 and a card block 15 are fixedly connected to the outer surface of the connecting base 10, a card slot 16 is provided on the outer surface of the sample collection bottle 1, a guide outlet 17 is provided at the bottom of the sample collection bottle 1, a right-angle guide tube 18 is fixedly connected to the inside of the visual window 3, a first piston 29 and a second piston 30 are disposed inside the right-angle guide tube 18, a first push rod 19 is fixedly connected to the outer surface of the first piston 29, a second push rod 20 is fixedly connected to the lower surface of the second piston 30, a pressure block 22 is fixedly connected to the end of the first push rod 19 away from the first piston 29, and a second rubber stopper 23 is fixedly connected to the end of the second push rod 20 away from the second piston 30.

[0029] The outer surface of the connecting rod 6 is sleeved inside the top cover 2 , and the top end of the connecting rod 6 is fixedly connected with a pull ring 7 , which is arranged above the top cover 2 .

[0030] The block 15 and the test card 11 are arranged on a side of the connection base 10 close to the sample collection bottle 1 , and the support rod 14 is arranged on a side of the connection base 10 away from the block 15 and the test card 11 .

[0031] The outer surface of the test card 11 is provided with a sample adding area 12 and a display area 13 , and the outer surface of the card block 15 is matched with the inner surface of the card slot 16 .

[0032] The right-angle tube 18 is disposed inside the sample chamber 28 , the first piston 29 is disposed at a horizontal portion of the right-angle tube 18 , and the second piston 30 is disposed at a vertical portion of the right-angle tube 18 .

[0033] The outer surfaces of the first push rod 19 and the second push rod 20 are both sleeved inside the right-angle tube 18 , and the outer surfaces of the first push rod 19 and the second push rod 20 pass through both ends of the right-angle tube 18 respectively.

[0034] The pressure block 22 is arranged outside the viewing window 3 , the outer surface of the second rubber stopper 23 is pressed and fitted with the inner surface of the outlet 17 , and a liquid column 21 is arranged at the interval between the first piston 29 and the second piston 30 .

[0035] The concave portion of the outer surface of the pressing block 22 is rotatably connected to a limiting rod 25 , the outer surface of the viewing window 3 is fixedly connected to a first magnetic sheet 24 , and the end of the limiting rod 25 is fixedly connected to a second magnetic sheet 26 .

[0036] A method for using a sampling and detection integrated device for detecting Helicobacter pylori antigens comprises the following steps: when sampling, unscrewing and removing the top cover 2 from the sample collection bottle 1, adding a diluent into the sample collection bottle 1, inserting the threaded portion 27 on the hollow sampling rod 4 into the stool sample, using the groove on the threaded portion 27 to obtain an appropriate amount of sample, and then placing the hollow sampling rod 4 into the sample cavity 28. If the stool is watery, pulling the pull ring 7 upwards, the first rubber stopper 5 slides along the inside of the hollow sampling rod 4, so that the hollow sampling rod 4 absorbs the watery stool. During the test, place the sample collection bottle 1 on the table, rotate the connecting seat 10 to a horizontal state, at which time the sample adding area 12 is just below the outlet 17, press the pressing block 22, so that the second push rod 20 pushes the second rubber stopper 23, the outlet 17 is opened, and the sample solution in the sample cavity 28 drips into the sample adding area 12. After the test card 11 is left to stand for fifteen minutes, the result of the display area 13 is observed.

[0037] Regarding the test results, the display area 13 of the test card 11 includes a test area (T) and a quality control area (C). If a red reaction line appears in both the test area (T) and the quality control area (C), it indicates a positive result and it is recommended to seek medical attention for a clear diagnosis; if only a red reaction line appears in the quality control area (C), it indicates a negative result and it is necessary to continue to maintain good dietary hygiene habits; if no red reaction line appears in the quality control area (C), it indicates that the test is invalid and it is recommended to retest.

[0038] Before collecting normal feces through the hollow sampling rod 4, the first rubber stopper 5 is placed at the bottom end of the inner surface of the hollow sampling rod 4, and the bottom of the first rubber stopper 5 is flush with the bottom of the hollow sampling rod 4 to prevent the collected feces from entering the hollow cavity of the hollow sampling rod 4. When collecting loose feces, the pull ring 7 is lifted upward, so that the connecting rod 6 drives the first rubber stopper 5 to move upward along the hollow cavity of the hollow sampling rod 4, and the hollow cavity of the hollow sampling rod 4 generates negative pressure, so that the loose feces are sucked into the hollow cavity of the hollow sampling rod 4, thereby realizing the sampling of loose feces.

[0039] Through the clamping block 15 and the clamping slot 16, the clamping block 15 is always clamped in the clamping slot 16 during the sampling process, and the connecting seat 10 is on the outer surface of the sample collection bottle 1, so that the whole device is easy to hold, and it is convenient to shake the sample in the sample collection bottle 1 after sampling. During the detection, the sample collection bottle 1 is first placed on the table, and the supporting rod 14 is pulled to make the clamping block 15 leave the clamping slot 16, and the connecting seat 10 is rotated to a horizontal state. The supporting rod 14 is on the table to provide support for the connecting seat 10, so that the connecting seat 10 remains in a horizontal state, ensuring the stability of the test card 11 and facilitating the detection.

[0040] Through the limiting rod 25, during the sampling process before detection, the second rubber stopper 23 is always blocked in the outlet 17, and the limiting rod 25 also rests on the visual window 3. The second magnetic piece 26 at the end of the limiting rod 25 is adsorbed together with the first magnetic piece 24 on the visual window 3, so that the limiting rod 25 clamps the pressing block 22 to avoid accidentally pressing the pressing block 22 and opening the outlet 17 in advance. When the sample solution needs to be discharged, the limiting rod 25 is rotated downward to make the second magnetic piece 26 leave the first magnetic piece 24, so that the limiting rod 25 naturally hangs down, releasing the restriction on the pressing block 22, and then pressing the pressing block 22 again. Since the first piston 29 and the second piston 30 are filled with a liquid column 21, the first piston 29 squeezes the liquid column 21 under the push of the first push rod 19, and the liquid column 21 is squeezed by the thrust of the second piston 30, so that the second push rod 20 drives the second rubber stopper 23 to move downward, the outlet 17 is opened, and the sample solution falls into the sample addition area 12 through the outlet 17.

[0041] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A sampling and detection integrated device for Helicobacter pylori antigen detection, comprising a sample collection bottle (1), characterized in that: The top of the sample collection bottle (1) is threadedly connected to a top cover (2), the outer surface of the sample collection bottle (1) is provided with a visual window (3), the top of the inner surface of the top cover (2) is fixedly connected to a hollow sampling rod (4), the bottom end of the inner surface of the hollow sampling rod (4) is provided with a first rubber stopper (5), the top of the first rubber stopper (5) is fixedly connected to a connecting rod (6), the outer surface of the hollow sampling rod (4) is provided with a threaded portion (27), the bottom of the sample collection bottle (1) is provided with a groove (8), the interior of the sample collection bottle (1) is provided with a sample cavity (28), the inner surface of the groove (8) is fixedly connected to a fixed shaft (9), the outer surface of the fixed shaft (9) is rotatably connected to a connecting seat (10), and the interior of the connecting seat (10) is provided with a test card (11), The outer surface of the connecting seat (10) is fixedly connected to a supporting rod (14) and a clamping block (15); the outer surface of the sample collection bottle (1) is provided with a clamping groove (16); the bottom of the sample collection bottle (1) is provided with a guide outlet (17); the interior of the visual window (3) is fixedly connected to a right-angled conduit (18); the interior of the right-angled conduit (18) is provided with a first piston (29) and a second piston (30); the outer surface of the first piston (29) is fixedly connected to a first push rod (19); the lower surface of the second piston (30) is fixedly connected to a second push rod (20); the end of the first push rod (19) away from the first piston (29) is fixedly connected to a pressure block (22); the end of the second push rod (20) away from the second piston (30) is fixedly connected to a second rubber stopper (23).

2. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The outer surface of the connecting rod (6) is sleeved inside the top cover (2), and the top end of the connecting rod (6) is fixedly connected with a pull ring (7), and the pull ring (7) is arranged above the top cover (2).

3. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The card block (15) and the test card (11) are arranged on a side of the connection seat (10) close to the sample collection bottle (1), and the support pull rod (14) is arranged on a side of the connection seat (10) away from the card block (15) and the test card (11).

4. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The outer surface of the test card (11) is provided with a sample adding area (12) and a display area (13), and the outer surface of the card block (15) is adapted to the inner surface of the card slot (16).

5. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The right-angle tube (18) is arranged inside the sample chamber (28), the first piston (29) is arranged at the horizontal part of the right-angle tube (18), and the second piston (30) is arranged at the vertical part of the right-angle tube (18).

6. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The outer surfaces of the first push rod (19) and the second push rod (20) are both sleeved inside the right-angled conduit (18), and the outer surfaces of the first push rod (19) and the second push rod (20) respectively pass through both ends of the right-angled conduit (18).

7. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The pressure block (22) is arranged outside the visual window (3), the outer surface of the second rubber stopper (23) is pressed and matched with the inner surface of the outlet (17), and a liquid column (21) is arranged at the interval between the first piston (29) and the second piston (30).

8. The sampling and detection integrated device for Helicobacter pylori antigen detection according to claim 1, characterized in that: The recessed portion of the outer surface of the pressing block (22) is rotatably connected to a limiting rod (25), the outer surface of the viewing window (3) is fixedly connected to a first magnetic sheet (24), and the end of the limiting rod (25) is fixedly connected to a second magnetic sheet (26).

9. A method for using a sampling and detection integrated device for Helicobacter pylori antigen detection, characterized in that: The method comprises the following steps: when sampling, unscrewing and removing the top cover (2) from the sample collection bottle (1), adding a diluent into the sample collection bottle (1), inserting the threaded portion (27) on the hollow sampling rod (4) into the fecal sample, obtaining an appropriate amount of sample by using the groove on the threaded portion (27), and then placing the hollow sampling rod (4) into the sample cavity (28); if the feces is loose, pulling the pull ring (7) upward, and the first rubber stopper (5) sliding along the inside of the hollow sampling rod (4) so ​​that the hollow sampling rod (4) absorbs loose feces, and placing the hollow sampling rod (4) into the sample cavity (28); 8) and then press down the pull ring (7) to squeeze the loose stool into the sample cavity (28), tighten the top cover (2) and shake it evenly; during the test, place the sample collection bottle (1) on the table, and then rotate the connecting seat (10) to a horizontal state. At this time, the sample adding area (12) is just below the outlet (17). Press the pressing block (22) so that the second push rod (20) pushes the second rubber stopper (23), the outlet (17) is opened, and the sample solution in the sample cavity (28) drips into the sample adding area (12). After the test card (11) is left to stand for fifteen minutes, the result of the display area (13) is observed.

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