Cleaning and separating device and cleaning and separating method

Abstract

The invention relates to the technical field of in-vitro diagnosis automation equipment, and discloses a cleaning and separating device and a cleaning and separating method.The cleaning and separating device comprises a base, a rotating mechanism, a fixing disc, a liquid injection plate, a liquid suction mechanism and a uniform mixing mechanism; the rotating mechanism comprises a rotating driving part and a rotating disc, the rotating disc is movably arranged on the base, the rotating disc is provided with a plurality of supporting parts, and the rotating driving part is connected with the rotating disc and used for driving the supporting parts to sequentially rotate to the corresponding operation positions; the fixed disc is arranged on the base and located at the lower end of the rotating disc, and a magnetic part is arranged on the fixed disc; the liquid injection plate is arranged on the base and located at the upper end of the rotating disc, and a plurality of liquid injection parts are arranged on the liquid injection plate. By optimizing the structures and mounting positions of all parts of the cleaning and separating device, the overall structure is simplified, the size is small, and the cleaning and separating device has good application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnostic automation equipment, and in particular to a cleaning and separation device and a cleaning and separation method. Background Art

[0002] In a fully automatic chemiluminescence immunoassay, in order to separate free markers from immune complex markers, a cleaning and separation device is usually used to remove unbound components in the reactants, and then the luminescence reaction is completed based on the immune complex containing the marker.

[0003] The cleaning and separation process includes injecting cleaning buffer into the reaction cup, resuspending the magnetic beads, adsorbing the magnetic beads, and absorbing the unbound components (i.e., waste liquid) in the reactants. The immune complex containing the marker is bound to the magnetic beads. During cleaning, the magnetic beads are adsorbed to the wall of the cup under the action of the magnetic field, and the unreacted components are sucked away by the liquid aspiration needle along with the liquid in the reaction cup. Repeated cleaning (stages) leaves the magnetic beads and the immune complex containing the marker on them in the reaction cup.

[0004] However, the existing cleaning and separation device has a complex structure, resulting in a large size. Summary of the invention

[0005] In view of this, the present invention provides a cleaning and separation device and a cleaning and separation method to solve the problem that the existing cleaning and separation device has a complex structure and results in a large volume.

[0006] In a first aspect, the present invention provides a cleaning and separation device, comprising:

[0007] Base;

[0008] The rotating mechanism comprises a rotating drive member and a rotating disk, wherein the rotating disk is movably arranged on the base, the rotating disk is provided with a plurality of supporting parts, each of the supporting parts is suitable for carrying a reaction container, and the rotating drive member is connected to the rotating disk and is used to drive each of the supporting parts to rotate to a corresponding operating position in sequence;

[0009] A fixed disk, arranged on the base and located at the lower end of the rotating disk, the fixed disk being provided with a magnetic member;

[0010] A liquid injection plate, arranged on the base and located at the upper end of the rotating disk, the liquid injection plate being provided with a plurality of liquid injection parts;

[0011] A liquid suction mechanism, disposed at the upper end of the liquid injection plate, for sucking liquid from the reaction container;

[0012] The mixing mechanism is arranged on the base and located at the lower end of the rotating disk. The mixing mechanism is provided with a plurality of mixers, and the mixers are used to mix the liquid in the reaction container.

[0013] Beneficial effects: The cleaning and separation device of the present invention drives the rotating disk through the rotating driving part, drives the reaction container on the support part to move to each operating position, performs liquid injection, magnetic suction, liquid absorption, mixing and cycles for multiple times in sequence, and then injects the substrate. It can continuously complete the cleaning and separation of reaction containers of various systems with high work efficiency, simplifies the overall structure of the cleaning and separation device, thereby reducing the volume of the cleaning and separation device, and has good application prospects.

[0014] In an optional embodiment, the liquid suction mechanism comprises:

[0015] A liquid absorption plate, which is arranged on the liquid injection plate through a guide rail, and a plurality of liquid absorption needles are arranged on the liquid absorption plate at intervals corresponding to the operating positions;

[0016] The lifting assembly is arranged between the liquid absorption plate and the liquid injection plate, and is used to drive the liquid absorption plate to slide up and down along the guide rail, so as to drive the liquid absorption needle to pass through the liquid injection plate and then enter and exit the reaction container to absorb the waste liquid in the reaction container.

[0017] Beneficial effect: The lifting assembly drives the liquid absorption plate to slide up and down along the guide rail, so as to drive the liquid absorption needle to pass through the liquid injection plate and then enter and exit the reaction container to absorb the waste liquid in the reaction container. Multiple liquid absorption needles are arranged on the liquid absorption plate and rise and fall synchronously to simplify the structure and reduce the control difficulty.

[0018] In an optional embodiment, the magnetic member has a avoidance portion for avoiding the mixer.

[0019] Beneficial effect: The magnetic part avoids the mixer, which can prevent the magnetic beads from gathering at the operating position of the mixer, thereby reducing the loss of magnetic beads and preventing the magnetic beads from affecting the mixing effect.

[0020] In an optional embodiment, the mixing mechanism includes:

[0021] A mixing driving member, disposed on the base;

[0022] The mounting plate, the mixing driving member is used to drive the mounting plate to move up and down relative to the base, and a plurality of mixers are arranged on the mounting plate at intervals.

[0023] Beneficial effect: A mixing drive is used to control the up and down movement of the mounting plate, so as to drive multiple mixers to move up and down synchronously at the same time, so that the mixers are close to or away from the reaction container and mix the reaction container, which simplifies the driving structure of the mixer and reduces the control difficulty of the mixer, thereby facilitating improving the work efficiency of cleaning and separation.

[0024] In an optional embodiment, the driving shaft of the mixing drive member is away from the base and connected to the mounting plate.

[0025] Beneficial effect: By separating the driving shaft of the mixing driving member from the base and connecting it to the mounting plate, it is convenient for the mixing driving member to drive the mounting plate to move up and down.

[0026] In an optional embodiment, the plurality of injection parts include a first substrate injection part and / or a second substrate injection part.

[0027] Beneficial effects: The substrate is injected into the reaction container through the first substrate injection part and / or the second substrate injection part, and the type of substrate in the first substrate injection part and / or the second substrate injection part is selected, which can meet the use requirements of various chemiluminescent reaction systems and has a wide application.

[0028] In an optional embodiment, a mixer is provided below the second substrate injection portion.

[0029] Beneficial effect: By arranging a mixer below the second substrate injection portion, the reaction container after the substrate is injected can be effectively mixed.

[0030] In an optional embodiment, the cleaning and separation device is respectively provided with three-stage liquid filling positions, three-stage magnetic suction positions and three-stage liquid suction positions, each stage of the magnetic suction positions is composed of a plurality of the magnetic parts, and each stage of the liquid suction positions is arranged above the last magnetic part of each stage of the magnetic suction positions.

[0031] Beneficial effect: Setting three-stage liquid injection positions, three-stage magnetic suction positions and three-stage liquid suction positions can clean and separate the magnetic beads in the reaction container three times, and the cleaning and separation process is more thorough. Setting each liquid suction position above the last magnetic piece of each magnetic suction position can save the space occupied by the three liquid suction positions, and at the same time facilitate the rapid suction of waste liquid after the magnetic suction is completed.

[0032] In a second aspect, the present invention further provides a cleaning and separation method, comprising:

[0033] According to the preset intervals, the rotary drive member is controlled to drive each support part to rotate to the corresponding operating position in sequence;

[0034] In the in-and-out position, the reaction container is loaded into or taken out of the support portion;

[0035] Rotate to the first liquid injection position, and control the liquid injection part to inject the cleaning liquid into the reaction container;

[0036] Rotate to the first magnetic attraction position to absorb the magnetic beads in the reaction container through the magnetic member;

[0037] Rotate to the first stage liquid suction position to control the liquid suction mechanism to suck the waste liquid in the reaction container;

[0038] Rotate to the second liquid injection position, control the liquid injection part to inject cleaning liquid into the reaction container, and then control the mixer to mix the liquid in the reaction container;

[0039] Rotate to the second magnetic attraction position to absorb the magnetic beads in the reaction container through the magnetic member;

[0040] Rotating to the second stage liquid suction position, controlling the liquid suction mechanism to suck the waste liquid in the reaction container;

[0041] Rotate to the third liquid injection position, control the liquid injection part to inject cleaning liquid into the reaction container, and then control the mixer to mix the liquid in the reaction container;

[0042] Rotate to the third magnetic attraction position to absorb the magnetic beads in the reaction container through the magnetic member;

[0043] The liquid injection part is rotated to the third stage liquid suction position to control the liquid injection part to inject the cleaning liquid into the reaction container.

[0044] Beneficial effects: The cleaning and separation method of the present invention drives the rotating disk through a rotating driving part, drives the reaction container on the support part to move to each operating position, and performs liquid injection, magnetic suction, liquid absorption, mixing and circulation for multiple times in sequence, injects the substrate, forms multi-stage cleaning and separation, and can continuously complete the cleaning and separation of reaction containers of various systems, has high working efficiency, simplifies the overall structure of the cleaning and separation device, thereby reducing the volume of the cleaning and separation device, and has good application prospects.

[0045] In an optional implementation, it also includes:

[0046] Rotate to the first substrate injection position and / or the second substrate injection position, and according to the chemiluminescent reaction system, control the first substrate injection position and the second substrate injection position to inject corresponding substrates into the reaction container respectively.

[0047] Beneficial effects: According to different chemiluminescent reaction systems, the corresponding substrate is injected into the reaction container. It is compatible with the detection and analysis of various chemiluminescent reaction systems, has a wide range of applications, and has a good user experience. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0049] Figure 1It is a structural schematic diagram of a cleaning and separation device according to an embodiment of the present invention;

[0050] Figure 2 A schematic diagram of a partial structure of a cleaning and separation device according to an embodiment of the present invention;

[0051] Figure 3 Another partial structural schematic diagram of a cleaning and separation device according to an embodiment of the present invention;

[0052] Figure 4 It is a schematic diagram of an operating position of a cleaning and separation device according to an embodiment of the present invention.

[0053] Description of reference numerals:

[0054] 1. Base; 101. In and out position; 102. First-stage liquid injection position; 103. First-stage magnetic suction position; 104. First-stage liquid suction position; 105. Second-stage liquid injection position; 106. Second-stage magnetic suction position; 107. Second-stage liquid suction position; 108. Third-stage liquid injection position; 109. Third-stage magnetic suction position; 110. Third-stage liquid suction position; 111. First substrate liquid injection position; 112. Second substrate liquid injection position; 2. Rotation mechanism; 201. Rotation Driving member; 202, rotating disk; 3, fixed disk; 301, magnetic member; 4, liquid injection plate; 5, liquid injection part; 501, first substrate liquid injection part; 502, second substrate liquid injection part; 6, liquid aspiration mechanism; 601, liquid aspiration plate; 602, guide rail; 603, liquid aspiration needle; 604, lifting assembly; 7, mixing mechanism; 701, mixer; 702, mixing driving member; 703, mounting plate; 704, mounting fixture; 8, reaction vessel. DETAILED DESCRIPTION

[0055] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0056] Combine the following Figures 1 to 4 , describing an embodiment of the present invention.

[0057] According to an embodiment of the present invention, on the one hand, Figure 1As shown, a cleaning and separation device is provided, which mainly includes: a base 1, a rotating mechanism 2, a fixed disk 3, a liquid injection plate 4, a liquid suction mechanism 6 and a mixing mechanism 7. The base 1 is provided with multiple operating positions. The rotating mechanism 2 includes a rotating driving member 201 and a rotating disk 202, the rotating disk 202 is movably arranged on the base 1, and a plurality of supporting parts are arranged at intervals on the peripheral side of the end face of the rotating disk 202, and each supporting part is suitable for carrying a reaction container 8. The rotating driving member 201 is connected to the rotating disk 202, and is used to drive each supporting part to rotate to the corresponding operating position in sequence. The fixed disk 3 is arranged on the base 1 and is located at the lower end of the rotating disk 202, and a magnetic member 301 is arranged around the corresponding operating positions of the fixed disk 3. The liquid injection plate 4 is arranged on the base 1 and is located at the upper end of the rotating disk 202, and a plurality of liquid injection parts 5 are arranged on the peripheral side of the end face of the liquid injection plate 4 corresponding to the supporting part. The liquid suction mechanism 6 is arranged at the upper end of the liquid injection plate 4, and is used to suck liquid from the reaction container 8. The mixing mechanism 7 is disposed on the base 1 and is located at the lower end of the rotating disk 202 . The mixing mechanism 7 is provided with a plurality of mixers 701 , and the mixers 701 are used to mix the liquid in the reaction container 8 .

[0058] The cleaning and separation device provided in the embodiment of the present invention drives the rotating disk 202 by the rotating driving member 201, thereby driving the reaction container 8 on the support part to move to various operating positions, and sequentially performs liquid injection, magnetic suction, liquid aspiration, mixing and cycles for multiple times, and then injects the substrate. It can continuously complete the cleaning and separation of reaction containers 8 of various systems, has high working efficiency, simplifies the overall structure of the cleaning and separation device, thereby reducing the volume of the cleaning and separation device, and has good application prospects.

[0059] Specifically, a support shaft is provided on the base 1, and the fixed disk 3, the rotating disk 202 and the liquid injection plate 4 are sequentially sleeved on the support shaft from bottom to top. The fixed disk 3 is used to fix the magnetic member 301, and the fixed disk 3 and the liquid injection plate 4 are fixedly arranged relative to the base 1, and the rotating disk 202 can rotate around the support shaft under the drive of the rotating driving member 201. The multiple operating positions on the base 1 include an entry and exit position 101, multiple liquid injection positions and multiple magnetic suction positions, and the specific number can be selected and set according to actual conditions, for example, three liquid injection positions and three magnetic suction positions are set.

[0060] It should be noted that the embodiment of the present invention does not limit the rotating drive component 201, and any existing structure can be selected as needed. For example, the rotating drive component 201 includes a motor and a conveyor belt. The output shaft of the motor is sleeved on one end of the conveyor belt, and the lower end of the support shaft is sleeved on the other end of the conveyor belt through the second gear. The conveyor belt is driven by the motor to rotate, and the transmission belt in turn drives the second gear and the support shaft to rotate. The support shaft drives the rotating disk 202 to rotate, and finally each support part is rotated to the corresponding operating position in turn.

[0061] In addition, the present invention does not limit the liquid aspiration mechanism 6 , as long as the liquid aspiration mechanism 6 can complete the liquid aspiration function for the waste liquid in the reaction container 8 .

[0062] In one embodiment, Figure 2 As shown, the liquid suction mechanism 6 mainly includes: a liquid suction plate 601 and a lifting assembly 604. The liquid suction plate 601 is arranged on the liquid injection plate 4 through a guide rail 602, and a plurality of liquid suction needles 603 are arranged at intervals corresponding to the operating positions on the liquid suction plate 601. The lifting assembly 604 is arranged between the liquid suction plate 601 and the liquid injection plate 4, and is used to drive the liquid suction plate 601 to slide up and down along the guide rail 602, so as to drive the liquid suction needle 603 to pass through the liquid injection plate 4 and then enter and exit the reaction container 8 to suck the waste liquid in the reaction container 8. A through hole for passing the liquid suction needle 603 is provided at the position corresponding to the liquid suction needle 603 on the liquid injection plate 4.

[0063] In the embodiment of the present invention, the lifting assembly 604 drives the liquid absorbing plate 601 to slide up and down along the guide rail 602, so as to drive the liquid absorbing needle 603 to pass through the liquid injection plate 4 and then enter and exit the reaction container 8 to absorb the waste liquid in the reaction container 8. Multiple liquid absorbing needles 603 are arranged on the liquid absorbing plate 601 and rise and fall synchronously to simplify the structure and reduce the control difficulty.

[0064] Further, in one embodiment, the magnetic member 301 is located outside the fixed disk 3, and the magnetic member 301 is arranged outside the fixed disk 3 for easy use. The magnetic member 301 can be a plurality of permanent magnets embedded around the fixed disk 3, or a magnetic ring sleeved on the outer periphery of the fixed disk 3.

[0065] Furthermore, in one embodiment, the magnetic member 301 has a avoiding portion for avoiding the mixer 701. The magnetic member 301 avoids the mixer 701, which can prevent the magnetic beads from gathering at the operating position of the mixer 701, thereby reducing the loss of magnetic beads and preventing the magnetic beads from affecting the mixing effect.

[0066] Specifically, the magnetic member 301 is provided with a groove to avoid the mixer 701, the liquid injection part 5 and the mixer 701 are arranged up and down, the reaction container 8 is located in the groove, the mixer 701 is located below the reaction container 8, and the liquid injection part 5 is located above the reaction container 8. When the reaction container 8 rotates to the groove of the magnetic member 301, the liquid injection part 5 injects the cleaning liquid into the reaction container 8, and then the mixer 701 mixes the cleaning liquid in the reaction container 8, without rotating the reaction container 8, the operation time is short, and the operation efficiency is high.

[0067] In one embodiment, Figure 3 As shown, the mixing mechanism 7 mainly includes: a mixing drive 702 and a mounting plate 703. The mixing drive 702 is arranged on the top of the base 1. The driving shaft of the mixing drive 702 is away from the base 1 and connected to the mounting plate 703, and is used to drive the mounting plate 703 to move up and down relative to the base 1. A plurality of mixers 701 are arranged on the mounting plate 703 at intervals.

[0068] The embodiment of the present invention uses a mixing drive 702 to control the installation plate 703 to move up and down, so as to simultaneously drive multiple mixers 701 to move up and down synchronously, so that the mixer 701 is close to or away from the reaction container 8, and the reaction container 8 is mixed, which simplifies the driving structure of the mixer 701 and reduces the control difficulty of the mixer 701, thereby facilitating the improvement of the working efficiency of cleaning and separation. By separating the driving shaft of the mixing drive 702 from the base 1 and connecting it to the installation plate 703, it is convenient for the mixing drive 702 to drive the installation plate 703 to move up and down.

[0069] It should be noted that the embodiment of the present invention does not limit the structure of the mixing drive member 702, and any existing structure can be selected as needed, as long as the mixing drive member 702 can drive the mounting plate 703 to move up and down relative to the base 1. For example, the mixing drive member 702 is a telescopic motor, and the driving shaft of the mixing drive member 702 drives the mounting plate 703 to move up and down relative to the base 1 by telescoping. Alternatively, the mixing drive member 702 is a screw motor, and the driving shaft of the mixing drive member 702 is a screw, which is threadedly connected to the mounting plate 703, and the screw motor is rotated to drive the mounting plate 703 to move up and down relative to the base 1.

[0070] Further, in one embodiment, a plurality of mixers 701 correspond to one operating position of the partial liquid injection part 5. A plurality of mixers 701 correspond to one operating position of the partial liquid injection part 5, which can reduce the space occupied by the mixer 701 and improve the space utilization.

[0071] In some other embodiments, the mixer 701 may also be arranged in a staggered manner with respect to the liquid injection part 5 .

[0072] Further, in one embodiment, if Figure 3 As shown, a plurality of mounting fixtures 704 are provided at intervals on the top of the mounting plate 703, and a plurality of mixers 701 are mounted one by one on the plurality of mounting fixtures 704. The mixer 701 is fixed on the top of the mounting plate 703 by the mounting fixture 704, with precise positioning, stable mixing, and convenient installation and removal of the mixer 701.

[0073] In one embodiment, the plurality of support portions are a plurality of bearing holes spaced apart along the circumference of the rotating disk 202, and the plurality of bearing holes are used to carry a plurality of reaction vessels 8, wherein at least one bearing hole is used to enter and exit at least one reaction vessel 8. The operation position corresponding to the bearing hole for entering and exiting the reaction vessel 8 is recorded as the entry and exit position 101. The rotating disk 202 is provided with bearing holes as support portions, which has a simple structure and is conducive to reducing the cost of use. Reaction vessels 8 of different systems can continuously enter and exit the rotating disk 202 from the bearing holes corresponding to the entry and exit position 101, so as to realize continuous detection and analysis of reaction vessels 8 of different chemiluminescent reaction systems, with better compatibility and improved efficiency of cleaning and separation.

[0074] In one embodiment, the plurality of injection parts 5 include a first substrate injection part 501 and / or a second substrate injection part 502. The substrate is respectively injected into the reaction container 13 through the first substrate injection part 501 and / or the second substrate injection part 502, and the substrate type of the first substrate injection part 501 and / or the second substrate injection part 502 is selected to meet the use requirements of various chemiluminescent reaction systems, and has a wide range of applications.

[0075] Further, in one embodiment, different substrates are placed in the first substrate injection part 501 and the second substrate injection part 502, or the same substrate is placed in the first substrate injection part 501 and the second substrate injection part 502. The first substrate injection part 501 and the second substrate injection part 502 are placed with the same or different substrates in advance to meet the detection needs of different chemiluminescent systems.

[0076] Chemiluminescence is classified according to the reaction system, mainly including indirect enzymatic chemiluminescence, such as AP (alkaline phosphatase) and HRP (horseradish peroxidase), and direct chemiluminescence, such as AE (acridinium ester) and electrochemiluminescence.

[0077] Furthermore, in one embodiment, a mixer 701 is provided below the second substrate injection portion 502. By providing a mixer 701 below the second substrate injection portion 502, the reaction container 8 after the substrate is injected can be effectively mixed.

[0078] In one embodiment, the cleaning and separation device is respectively provided with three-stage liquid injection positions, three-stage magnetic suction positions and three-stage liquid suction positions, each stage of the magnetic suction position is composed of a plurality of magnetic parts 301, and each stage of the liquid suction position is arranged above the last magnetic part 301 of each stage of the magnetic suction position. In addition, each stage of the liquid injection position is composed of a liquid injection part 5, and each stage of the liquid suction position is composed of a liquid suction needle 603. By providing three-stage liquid injection positions, three-stage magnetic suction positions and three-stage liquid suction positions, the magnetic beads in the reaction container 8 can be cleaned and separated three times, and the cleaning and separation process is more thorough. By setting each stage of the liquid suction position above the last magnetic part 301 of each stage of the magnetic suction position, the space occupied by the three liquid suction positions can be saved, and at the same time, it is beneficial to quickly suck out the waste liquid after the magnetic suction is completed.

[0079] Specifically, Figure 4As shown, twenty-one operating positions are arranged on the cleaning and separation device, including in and out position 101, first-stage liquid injection position 102, first-stage magnetic suction position 103, first-stage liquid suction position 104, second-stage liquid injection position 105, second-stage magnetic suction position 106, second-stage liquid suction position 107, third-stage liquid injection position 108, third-stage magnetic suction position 109, third-stage liquid suction position 110, first substrate liquid injection position 111 and second substrate liquid injection position 112. Among them, a liquid injection part 5 is arranged above the first-stage liquid injection position 102, the second-stage liquid injection position 105 and the third-stage liquid injection position 108. The first-stage magnetic attraction position 103, the second-stage magnetic attraction position 106 and the third-stage magnetic attraction position 109 are arranged at intervals respectively, the first-stage liquid suction position 104 and the second-stage liquid filling position 105 are arranged between the first-stage magnetic attraction position 103 and the second-stage magnetic attraction position 106, and the second-stage liquid suction position 107 and the third-stage liquid filling position 108 are arranged between the second-stage magnetic attraction position 106 and the third-stage magnetic attraction position 109.

[0080] Twenty-one bearing holes are correspondingly provided on the rotating disk 202, and the rotating disk 202 rotates once at a preset interval to make the supporting part progressively move to the next operating position.

[0081] In addition, the first-order magnetic suction position 103 can correspond to five bearing holes, each bearing hole is correspondingly provided with a permanent magnet, and the first-order suction position 104 is provided on the permanent magnet near the second-order liquid injection position 105. Similarly, the second-order suction position 107 is provided on the permanent magnet near the third-order liquid injection position 108. The third-order suction position 110 is provided on the permanent magnet near the first substrate liquid injection position 111. That is, the first suction needle 603 is provided above the permanent magnet near the second-order liquid injection position 105, the second suction needle 603 is provided above the permanent magnet near the third-order liquid injection position 108, and the third suction needle 603 is provided on the upper end of the permanent magnet near the first substrate liquid injection position 111.

[0082] The working principle of the embodiment of the present invention is as follows:

[0083] The reaction container 8 is first loaded onto the support portion of the rotating disk 202 from the entry and exit position 101 , and then advanced to the first-stage liquid filling position 102 , and the liquid filling portion 5 above the first-stage liquid filling position 102 injects the cleaning liquid into the reaction container 8 .

[0084] Progressively moving to the first-stage magnetic attraction position 103 , the magnetic beads in the reaction container 8 are adsorbed onto the cup wall under the action of the magnetic field.

[0085] When the liquid suction position 104 is reached, the first liquid suction needle 603 moves toward the reaction container 8 in a step-by-step manner driven by the lifting assembly 604, and simultaneously sucks the waste liquid in the reaction container 8. After descending to the bottom of the reaction container 8, the lifting assembly 604 drives the liquid suction needle 603 to move upward and leave the reaction container 8.

[0086] Progressively advancing to the second-stage liquid filling position 105, the liquid filling part 5 above the second-stage liquid filling position 105 injects the cleaning liquid into the reaction container 8. At the same time, the mixer 701 moves upward driven by the mixing driving member 702 to eccentrically mix the reaction container 8 and re-suspend the magnetic beads.

[0087] Progressively moving to the second-stage magnetic attraction position 106 , the magnetic beads in the reaction container 8 are adsorbed onto the cup wall under the action of the magnetic field.

[0088] Progressively advancing to the second stage liquid aspiration position 107, the second liquid aspiration needle 603 moves toward the reaction container 8 in a step-by-step manner driven by the lifting assembly 604, while aspirating the waste liquid in the reaction container 8. After descending to the bottom of the reaction container 8, the lifting assembly 604 drives the liquid aspiration needle 603 to move upward and leave the reaction container 8.

[0089] Progressively advancing to the third-stage liquid filling position 108, the liquid filling part 5 above the third-stage liquid filling position 108 injects the cleaning liquid into the reaction container 8. At the same time, the mixer 701 moves upward driven by the mixing driving member 702 to eccentrically mix the reaction container 8 and re-suspend the magnetic beads.

[0090] Progressively moving to the third magnetic attraction position 109 , the magnetic beads in the reaction container 8 are adsorbed onto the cup wall under the action of the magnetic field.

[0091] When the third liquid aspiration position 110 is reached, the third liquid aspiration needle 603 moves toward the reaction container 8 in a step-by-step manner driven by the lifting assembly 604, and simultaneously aspirates the waste liquid in the reaction container 8. After descending to the bottom of the reaction container 8, the lifting assembly 604 drives the liquid aspiration needle 603 to move upward and away from the reaction container 8.

[0092] Progressively advance to the first substrate injection position 111 , if the reaction container 8 is of AP and AE type, no substrate is injected; if the reaction container 8 is of HPR type, the first substrate injection part 501 injects the first substrate.

[0093] The liquid is gradually moved to the second substrate injection position 112. If the reaction container 8 is of AP type, the second substrate injection part 502 injects the second substrate. If the reaction container 8 is of AE type, the second substrate injection part 502 injects the pre-excitation liquid. If the reaction container 8 is of HPR type, the second substrate injection part 502 injects the third substrate. Afterwards, the mixer 701 moves upward under the drive of the mixing drive 702 to perform eccentric mixing on the reaction container 8.

[0094] Re-advance to the entry and exit position 101, remove the reaction container 8 from the support portion of the rotating disk 202, place a new reaction container 8 and repeat the above steps.

[0095] According to an embodiment of the present invention, on the other hand, a cleaning and separation method is also provided, comprising:

[0096] S100 , controlling the rotary drive member 201 to drive each support part to rotate to a corresponding operating position in sequence according to a preset interval.

[0097] S200, at the loading and unloading position 101, the reaction container 8 is loaded into or taken out from the supporting part.

[0098] S300 , rotating to the first-stage liquid injection position 102 , controlling the liquid injection unit 5 to inject cleaning liquid into the reaction container 8 .

[0099] S400 , rotating to the first-stage magnetic attraction position 103 , and adsorbing the magnetic beads in the reaction container 8 through the magnetic member 301 .

[0100] S500 , rotating to the first stage liquid suction position 104 , controlling the liquid suction mechanism 6 to suck the waste liquid in the reaction container 8 .

[0101] S600 , rotating to the second-stage liquid injection position 105 , controlling the liquid injection unit 5 to inject cleaning liquid into the reaction container 8 , and then controlling the mixer 701 to mix the liquid in the reaction container 8 .

[0102] S700 , rotating to the second-stage magnetic attraction position 106 , and adsorbing the magnetic beads in the reaction container 8 through the magnetic member 301 .

[0103] S800, rotating to the second stage liquid suction position 107, controlling the liquid suction mechanism 6 to suck the waste liquid in the reaction container 8;

[0104] S900 , rotating to the third-stage liquid injection position 108 , controlling the liquid injection unit 5 to inject cleaning liquid into the reaction container 8 , and then controlling the mixer 701 to mix the liquid in the reaction container 8 .

[0105] S1000 , rotating to the third-stage magnetic attraction position 109 , and adsorbing the magnetic beads in the reaction container 8 through the magnetic member 301 .

[0106] S1100 , rotating to the third stage liquid suction position 110 , controlling the liquid injection part 5 to inject the cleaning liquid into the reaction container 8 .

[0107] The cleaning and separation method of the present invention drives the rotating disk 202 by the rotating driving member 201, driving the reaction container 8 on the support part to move to each operating position, and sequentially performs liquid injection, magnetic attraction, liquid absorption, and mixing and cycles three times, injects the substrate, and forms a three-stage cleaning and separation. It can continuously complete the cleaning and separation of reaction containers 8 of various systems, has high working efficiency, simplifies the overall structure of the cleaning and separation device, thereby reducing the volume of the cleaning and separation device, and has good application prospects.

[0108] In one embodiment, a cleaning and separation method further comprises:

[0109] S1200 , rotating to the first substrate injection position 111 and the second substrate injection position 112 , and controlling the first substrate injection position 111 and the second substrate injection position 112 to inject corresponding substrates into the reaction container 8 respectively according to the chemiluminescent reaction system.

[0110] According to different chemiluminescent reaction systems, corresponding substrates are injected into the reaction container 8, which is compatible with the detection and analysis of various chemiluminescent reaction systems, has a wide range of applications, and has a good user experience.

[0111] In one embodiment, the first-stage magnetic suction position 103, the second-stage magnetic suction position 106 and the third-stage magnetic suction position 109 are arranged at intervals, and the first-stage liquid suction position 104 and the second-stage liquid filling position 105 are arranged between the first-stage magnetic suction position 103 and the second-stage magnetic suction position 106, and the second-stage liquid suction position 107 and the third-stage liquid filling position 108 are arranged between the second-stage magnetic suction position 106 and the third-stage magnetic suction position 109.

[0112] The first-stage liquid suction position 104 and the second-stage liquid filling position 105 are arranged between the first-stage magnetic suction position 103 and the second-stage magnetic suction position 106, and the second-stage liquid suction position 107 and the third-stage liquid filling position 108 are arranged between the second-stage magnetic suction position 106 and the third-stage magnetic suction position 109, so as to facilitate the reaction container 8 to quickly enter the next stage of cleaning and separation action, thereby helping to improve the working efficiency.

[0113] In one embodiment, a first-stage liquid suction position 104 is provided on the first-stage magnetic suction position 103, a second-stage liquid suction position 107 is provided on the second-stage magnetic suction position 106, and a third-stage liquid suction position 110 is provided on the third-stage magnetic suction position 109. The three liquid suction positions are respectively arranged on three magnetic suction positions, which can save the space occupied by the three liquid suction positions and facilitate the rapid suction of waste liquid after the magnetic suction is completed.

[0114] Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A cleaning and separation device, characterized in that: include: Base (1); The rotating mechanism (2) comprises a rotating drive member (201) and a rotating disk (202), wherein the rotating disk (202) is movably arranged on the base (1), the rotating disk (202) is provided with a plurality of supporting parts, each of the supporting parts is suitable for carrying a reaction container (8), and the rotating drive member (201) is connected to the rotating disk (202) and is used to drive each of the supporting parts to rotate to a corresponding operating position in sequence; A fixed disk (3) is arranged on the base (1) and is located at the lower end of the rotating disk (202), and the fixed disk (3) is provided with a magnetic part (301); A liquid injection plate (4) is arranged on the base (1) and located at the upper end of the rotating disk (202), and the liquid injection plate (4) is provided with a plurality of liquid injection parts (5); A liquid suction mechanism (6), disposed at the upper end of the liquid injection plate (4), and used for sucking liquid from the reaction container (8); The mixing mechanism (7) is arranged on the base (1) and is located at the lower end of the rotating disk (202). The mixing mechanism (7) is provided with a plurality of mixers (701), and the mixers (701) are used to mix the liquid in the reaction container (8).

2. The cleaning and separation device according to claim 1, characterized in that: The liquid suction mechanism (6) comprises: A liquid absorption plate (601) is arranged on the liquid injection plate (4) via a guide rail (602), and a plurality of liquid absorption needles (603) are arranged on the liquid absorption plate (601) at intervals corresponding to the operating positions; The lifting assembly (604) is arranged between the liquid absorption plate (601) and the liquid injection plate (4), and is used to drive the liquid absorption plate (601) to slide up and down along the guide rail (602), so as to drive the liquid absorption needle (603) to pass through the liquid injection plate (4) and then enter and exit the reaction container (8) to absorb the waste liquid in the reaction container (8).

3. The cleaning and separation device according to claim 2, characterized in that: The magnetic member (301) has a relief portion for avoiding the mixer (701).

4. The cleaning and separation device according to any one of claims 1 to 3, characterized in that: The mixing mechanism (7) comprises: A mixing driving member (702) is disposed on the base (1); The mounting plate (703) is used for driving the mounting plate (703) to move up and down relative to the base (1), and a plurality of the mixers (701) are arranged at intervals on the mounting plate (703).

5. The cleaning and separation device according to claim 4, characterized in that: The driving shaft of the mixing driving member (702) is away from the base (1) and is connected to the mounting plate (703).

6. The cleaning and separation device according to any one of claims 1 to 3, characterized in that: The plurality of liquid injection parts (5) include a first substrate liquid injection part (501) and / or a second substrate liquid injection part (502).

7. The cleaning and separation device according to claim 6, characterized in that: A mixer (701) is provided below the second substrate injection portion (502).

8. The cleaning and separation device according to any one of claims 1 to 3, characterized in that: The cleaning and separation device is respectively provided with three stages of liquid injection positions, three stages of magnetic suction positions and three stages of liquid suction positions, each stage of the magnetic suction positions is composed of a plurality of the magnetic parts (301), and each stage of the liquid suction positions is provided above the last magnetic part (301) of each stage of the magnetic suction positions.

9. A cleaning and separation method, characterized in that: include: According to a preset interval, the rotary drive member (201) is controlled to drive each support part to rotate to a corresponding operating position in sequence; At the entry and exit position (101), the reaction container (8) is loaded into or taken out of the support portion; Rotating to the first-stage liquid injection position (102), controlling the liquid injection part (5) to inject the cleaning liquid into the reaction container (8); Rotating to the first-stage magnetic attraction position (103), and adsorbing the magnetic beads in the reaction container (8) through the magnetic member (301); Rotating to the first stage liquid aspiration position (104), controlling the liquid aspiration mechanism (6) to aspirate the waste liquid in the reaction container (8); Rotate to the second-stage liquid injection position (105), control the liquid injection part (5) to inject cleaning liquid into the reaction container (8), and then control the mixer (701) to mix the liquid in the reaction container (8); Rotating to the second-stage magnetic attraction position (106), and adsorbing the magnetic beads in the reaction container (8) through the magnetic member (301); Rotating to the second stage liquid suction position (107), controlling the liquid suction mechanism (6) to suck the waste liquid in the reaction container (8); Rotate to the third liquid injection position (108), control the liquid injection part (5) to inject cleaning liquid into the reaction container (8), and then control the mixer (701) to mix the liquid in the reaction container (8); Rotating to the third magnetic attraction position (109), and adsorbing the magnetic beads in the reaction container (8) through the magnetic member (301); The liquid injection portion (5) is controlled to inject cleaning liquid into the reaction container (8) by rotating to the third liquid suction position (110).

10. The cleaning and separation method according to claim 9, characterized in that: Also includes: Rotate to the first substrate injection position (111) and / or the second substrate injection position (112), and according to the chemiluminescent reaction system, control the first substrate injection position (111) and the second substrate injection position (112) to respectively inject corresponding substrates into the reaction container (8).

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