Imaging method and system based on high-speed axial zooming technology

Abstract

The invention discloses an imaging method and system based on a high-speed axial zooming technology, and the method comprises the steps: obtaining the imaging demand information of a sample, and obtaining a demand signal; when the demand signal is imaging preview, a vibration exciter is started, a reflector is driven to axially scan a sample on a sample bin, and a camera is matched with the motion period of the vibration exciter to adjust the speed of a shutter and perform sampling imaging on the sample; and when the demand signal is rapid imaging, the vibration exciter is closed, and the camera adjusts the shutter speed in cooperation with the movement period of the sample bin and performs sampling imaging on the sample. By setting two speed imaging modes, the requirements for the axial resolution, the sampling rate, the sampling preview and the imaging time under different conditions can be met, and the imaging convenience and compatibility based on the high-speed axial zooming technology are improved.

Description

Technical Field

[0001] The invention relates to the technical field of light sheet microscope imaging based on high-speed axial zoom technology, in particular to an imaging method and system based on high-speed axial zoom technology. Background Art

[0002] The generation and scanning of light sheets in light sheet microscopy is the core link of light sheet microscopy imaging technology. The imaging system illuminates the sample under examination through the light sheet, and excites the fluorescence signal of the sample layer corresponding to the focal plane of the imaging objective lens in a targeted manner, and performs detection imaging in the orthogonal direction, thereby effectively avoiding the influence of the out-of-focus background on the imaging quality. It has the characteristics of high imaging contrast and natural optical tomography. By moving the relative position between the transparent sample and the static light sheet, the tissue in the focal plane of the sample is stimulated to emit a fluorescence signal, and the signal is captured by the imaging arm in the orthogonal direction of the focal plane. The system moves the sample axially, uses a digital camera to synchronously capture two-dimensional fluorescence images of different layers of the sample, establishes a three-dimensional data point set in the process of moving the sample, and uses graphics processing software to achieve three-dimensional visualization reconstruction of the sample.

[0003] In the existing technology, only scientific research systems have similar imaging modes. Axial illumination technology is to scan the light sheet in the illumination direction and synchronize the light sheet waist position with the camera's rolling shutter exposure area to reduce the impact of defocus information on imaging, thereby improving resolution. Its core lies in the performance parameters of the zoom system. In recent years, the zoom methods that have been widely used include piezo ceramic motors (Piezo Ceramics motor), voice coil motors (Voice-Coil motor), liquid lenses (ETL), etc., but these methods have their own defects in cost, stability, optical aberration caused by zoom, and zoom travel. Summary of the invention

[0004] To solve the above problems, the purpose of the present invention is to provide an imaging method, system and storage medium based on high-speed axial zoom technology. By setting two speed imaging modes, the requirements for axial resolution, sampling rate, sampling preview and imaging time in different situations are met, thereby improving the convenience and compatibility of imaging based on high-speed axial zoom technology.

[0005] The technical solution adopted by the present invention to solve the problem is:

[0006] In a first aspect, an embodiment of the present application provides an imaging method based on high-speed axial zoom technology, the method comprising: acquiring imaging requirement information of a sample and obtaining a requirement signal; when the requirement signal is an imaging preview, turning on the vibrator and driving the reflector to perform an axial scan of the sample on the sample bin, and the camera adjusts the shutter speed in accordance with the movement cycle of the vibrator and performs sampling and imaging of the sample; when the requirement signal is rapid imaging, turning off the vibrator, and the camera adjusts the shutter speed in accordance with the movement cycle of the sample bin and performs sampling and imaging of the sample.

[0007] In a second aspect, an embodiment of the present application provides a light sheet microscope imaging system based on high-speed axial zoom technology, comprising: an acquisition module, used to acquire imaging requirement information of a sample and obtain a requirement signal; a first sampling module, used to turn on the exciter when the requirement signal is an imaging preview, and drive the reflector to perform axial scanning of the sample on the sample bin, and the camera adjusts the shutter speed in accordance with the movement cycle of the exciter and performs sampling and imaging of the sample; a second sampling module, used to turn off the exciter when the requirement signal is rapid imaging, and the camera adjusts the shutter speed in accordance with the movement cycle of the sample bin and performs sampling and imaging of the sample.

[0008] In a third aspect, an embodiment of the present application provides an electronic device, comprising: a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein when the processor executes the computer program, the imaging method based on the high-speed axial zoom technology as described above is implemented.

[0009] In a fourth aspect, an embodiment of the present application provides a computer-readable storage medium storing a computer program, which, when executed by a processor, implements the imaging method based on the high-speed axial zoom technology as described above.

[0010] In the embodiment of the present application, by obtaining the imaging requirement information of the sample, and according to the imaging requirement information, turning on the vibrator to drive the reflector to perform axial scanning of the sample, the camera adjusts the shutter speed in accordance with the movement cycle of the vibrator and performs sampling imaging of the sample, and performs the specific position of the sample and retrograde standard imaging and the required signal preview; or turning off the vibrator, the camera adjusts the shutter speed in accordance with the movement cycle of the sample bin and performs sampling imaging of the sample, and performs fast high-definition imaging of large-sized samples. The two speed imaging modes can meet the requirements of axial resolution, sampling rate, sampling preview, and imaging time in different situations, and improve the convenience and compatibility of imaging based on high-speed axial zoom technology.

[0011] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of a flow chart of an imaging method based on high-speed axial zoom technology according to an embodiment of the present application;

[0013] Figure 2 yes Figure 1 A schematic diagram of a specific implementation process of another embodiment of step S1000;

[0014] Figure 3 yes Figure 1 A schematic diagram of a specific implementation process of another embodiment of step S2000;

[0015] Figure 4 yes Figure 1 A schematic diagram of a specific implementation process of another embodiment of step S3000;

[0016] Figure 5 This is a structural diagram of a light sheet microscope imaging system based on high-speed axial zoom technology in an embodiment of the present application;

[0017] Figure 6 yes Figure 5 A schematic diagram of the structure of the first sampling module and the second sampling module;

[0018] Figure 7 It is a structural schematic diagram of an electronic device provided in an embodiment of the present application. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solution and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0020] It should be understood that in the description of the embodiments of the present application, if there is a description of "first", "second", etc., it is only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the order of the indicated technical features. "At least one" means one or more, and "multiple" means two or more. "And / or" describes the association relationship of associated objects, indicating that three relationships may exist. For example, A and / or B can represent the situation where A exists alone, A and B exist at the same time, and B exists alone. Wherein A and B can be singular or plural. The character " / " generally indicates that the previous and next associated objects are in an "or" relationship. "At least one of the following" and similar expressions refer to any combination of these items, including any combination of single or plural items. For example, at least one of a, b and c can represent: a, b, c, a and b, a and c, b and c or a and b and c, where a, b, c can be single or multiple.

[0021] In addition, the technical features involved in each embodiment of the present application described below can be combined with each other as long as they do not conflict with each other.

[0022] The imaging method based on high-speed axial zoom technology involved in the embodiment of the present application is based on a light sheet microscope and a three-dimensional tissue imaging mode. By moving the relative position between the transparent sample and the static light sheet, the tissue in the focal plane of the sample is stimulated to emit a fluorescent signal, and the signal is captured by the imaging arm in the orthogonal direction of the focal plane. Then, by moving the sample axially, a digital camera is used to synchronously capture two-dimensional fluorescent images of different layers of the sample, and a three-dimensional data point set is established in the process of moving the sample. With the help of graphics processing software, a three-dimensional visualization reconstruction of the sample is achieved.

[0023] The imaging method based on high-speed axial zoom technology involved in the embodiment of the present application eliminates the spherical aberration and chromatic aberration caused by the system lens during the zooming process by designing a symmetrical optical path structure, thereby ensuring stable axial resolution under a large field of view. In the zooming method, a vibrator with lower cost and more outstanding performance indicators is adopted. By conjugating the focus of the light sheet to a free space position, the light sheet is axially scanned using a reflector, and the conjugate position of the light sheet also changes axially. At this time, the moving part of the axis zoom system is not an objective lens, but a reflector with a size greater than the cross-sectional area of ​​the light sheet mounted on the vibrator, which can effectively reduce the motion pressure of the scanning device, not only improve the imaging speed of the imaging system, but also greatly increase the travel distance of the zoom optical path. However, the existing imaging method based on high-speed axial zoom technology can only execute a single imaging acquisition method, and cannot adjust the working parameters between the camera, the vibrator and the sample chamber according to the actual imaging requirements to meet the requirements of axial resolution, sampling rate, sampling preview and imaging time in different situations, which affects the wide application of the imaging method based on high-speed axial zoom technology.

[0024] Based on the above, the embodiments of the present application provide an imaging method, system and storage medium based on high-speed axial zoom technology, which obtains the imaging requirement information of the sample and, according to the imaging requirement information, turns on the exciter to drive the reflector to perform axial scanning on the sample, and the camera adjusts the shutter speed in accordance with the movement cycle of the exciter and performs sampling and imaging on the sample, and performs the specific position of the sample and retrograde standard imaging and the required signal preview; or turns off the exciter, and the camera adjusts the shutter speed in accordance with the movement cycle of the sample bin and performs sampling and imaging on the sample, and performs fast high-definition imaging on large-sized samples. The two speed imaging modes can meet the requirements for axial resolution, sampling rate, sampling preview and imaging time in different situations, and improve the convenience and compatibility of imaging based on high-speed axial zoom technology.

[0025] See also Figure 1 , Figure 1 FIG. 2 shows a flow chart of an imaging method based on high-speed axial zoom technology provided by an embodiment of the present application. Figure 1 As shown, the imaging method based on high-speed axial zoom technology in the embodiment of the present application includes the following steps:

[0026] S1000, acquiring imaging requirement information of a sample and obtaining a requirement signal.

[0027] It is understandable that the imaging object based on high-speed axial zoom technology is a sample with a large surface area. Such samples usually need to be imaged quickly and with high resolution, and there is no need to preview the sample imaging; it can also be a sample with a small imaging area and a certain thickness. Such samples usually do not require high speed and accuracy, but need to preview the sample imaging. Therefore, different imaging modes are selected for samples of different sizes and different imaging requirements to meet the different imaging requirements of high-speed three-dimensional sampling and online sample observation, and to improve the imaging speed of the sample.

[0028] See also Figure 2 , Figure 2 FIG. 5 is a schematic diagram showing a specific implementation process of another embodiment of the above step S1000. Figure 2 As shown, the imaging method based on high-speed axial zoom technology in the embodiment of the present application includes the following steps:

[0029] S1100, acquiring imaging requirement information of the sample, including imaging area, scanning thickness and imaging speed of the sample.

[0030] It is understandable that samples with different imaging areas, scanning thicknesses and imaging speeds have different imaging requirements. Therefore, before performing the imaging operation, the imaging requirement information of the sample must be obtained so that the imaging method based on the high-speed axial zoom technology can meet the requirements of axial resolution, sampling rate, sampling preview and imaging time in different situations.

[0031] It can be understood that the imaging requirement information such as the imaging area, scanning thickness and imaging speed of the sample can be automatically acquired through the data acquisition system (DAQ), and can also be manually input through the client. The acquisition process and means of the imaging area, scanning thickness and imaging speed of the sample belong to the existing technology and will not be repeated here.

[0032] S1200, obtaining a demand signal according to the imaging area, the scanning thickness and the imaging speed.

[0033] It is understandable that by collecting and analyzing the imaging area, scanning thickness and imaging speed of the sample, it can be determined whether the sample needs to be imaged previewed, and then the demand signal can be output. In a specific application, when the imaging area of ​​the sample is less than the preset area threshold, the scanning thickness of the sample reaches the preset thickness threshold, and the imaging speed required by the sample is less than the preset speed threshold, it can be determined that the sample needs to be imaged previewed, that is, the demand signal is imaging preview, and the imaging mode of turning on the exciter is entered to facilitate finding the characteristic surface and characteristic structure of the sample and performing sampling imaging; when the imaging area of ​​the sample is greater than the preset area threshold, the scanning thickness of the sample is less than the preset thickness threshold, and the imaging speed required by the sample is greater than the preset speed threshold, it can be determined that the sample does not need to be imaged previewed, that is, the demand signal is fast imaging, and the imaging mode of turning off the exciter is entered to increase the imaging speed of the sample.

[0034] It is understandable that the demand signal includes but is not limited to imaging preview and fast imaging. In practical applications, it can also be defined according to specific imaging requirements so as to quickly and accurately obtain the demand signal according to the imaging requirement information and select the corresponding imaging mode.

[0035] It is understandable that in actual applications, whether the sample needs to be imaged and previewed can be directly input on the client according to user needs, so that the imaging requirement information can be set more flexibly and quickly, ensuring the compatibility of the imaging method based on high-speed axial zoom technology.

[0036] S1300, placing the sample on the sample chamber, wherein the sample chamber and the exciter are located on both sides of the polarization cube, and objective lenses are provided between the sample chamber and the exciter and the polarization cube, and the objective lenses form symmetrical light paths on both sides of the polarization cube.

[0037] It is understandable that in order to ensure that the sample is in a position convenient for the camera to collect imaging data during the imaging process, the sample needs to be fixed on the sample chamber so that the sample can move with the sample chamber to facilitate scanning and imaging of different positions of the sample.

[0038] It is understood that the positional relationship between the sample chamber, polarization cube, objective lens and exciter is as follows: Figure 6 The imaging method based on high-speed axial zoom technology of the present application mainly comprises an objective lens between the sample chamber and the polarization cube, and an objective lens between the exciter and the polarization cube, forming a symmetrical optical path.

[0039] In practical applications, a lens group is also provided between the sample chamber and the polarization cube to correct the spherical aberration and chromatic aberration generated by the reflector during its movement. The lens group optimizes different dispersion values ​​and lens shapes at different wavelengths to achieve minimum chromatic aberration. Specifically, the lens group is a set of achromatic doublets with the same focal length.

[0040] S2000, when the demand signal is imaging preview, turns on the vibrator and drives the reflector to perform axial scanning on the sample on the sample chamber. The camera adjusts the shutter speed according to the movement cycle of the vibrator and samples and images the sample.

[0041] It can be understood that the laser emits laser light into the polarization cube, and the polarization cube reflects most of the laser light into the right side of the symmetrical optical path. After being focused by the high-power objective lens 2, the focus falls on the front and back of the reflector. The reflector reflects the focused light beam back to the symmetrical optical path, and the light beam continues to travel from right to left. It passes through the lens group composed of lens 1, lens 2, lens 3, and lens 4 in sequence. The function of the lens group is to correct the spherical aberration and chromatic aberration generated by the reflector during the movement. It is also focused on the sample through the high-power objective lens 1. Since the position of the objective lens 1 that determines the convergence position of the light sheet and the preceding optical path are completely symmetrical with the position of the objective lens 2 that determines the position of the reflector. The front and rear focal positions of the reflector are also completely conjugate with the position of the sample. Therefore, moving the reflector along the symmetrical optical path defense line so that the light beam forms real focus and virtual focus at the front and rear positions of the reflector respectively is equivalent to changing the focal position of the original light beam in the modified optical path and moving the focal position at the front and rear positions of the reflector. Similarly, the position of the sample behind the objective lens 2 also moves back and forth after the original position. At this time, you only need to adjust the frequency and amplitude of the vibrator's forward and backward movement to make the camera's shutter movement direction match the vibrator's movement direction to achieve axial zoom technology.

[0042] In actual applications, the vibration generator is turned on, the reflector performs axial scanning on the sample in the sample chamber, and the camera adjusts the shutter speed in accordance with the vibration generator's motion cycle and samples and images the sample in a mode called standard sampling mode. The axial resolution of the above imaging system is up to 610 nanometers, which can be adjusted online according to imaging needs to meet the tomography requirements of different tissues of the sample.

[0043] See also Figure 3 , Figure 3 FIG. 2 shows a schematic diagram of a specific implementation process of another embodiment of the above step S2000. Figure 3 As shown, the imaging method based on high-speed axial zoom technology in the embodiment of the present application includes the following steps:

[0044] S2100, adjust the shutter speed so that the line scan rate of the camera is consistent with the movement period of the exciter.

[0045] It is understandable that since the vibrator is in the start-up state, it drives the reflector to move back and forth, and it is necessary to control the shutter movement direction of the camera to adapt to the movement direction of the vibrator to perform imaging and acquisition operations on the sample. At this time, the camera is excited by an external square wave signal, and the triangular wave signal excited by the vibrator comes from DAQ. The data acquisition frequency of the camera is consistent with the movement rate of the sample chamber, and the signal comes from DAQ control. For example, an image is collected according to the preset area (X1, Y1, W1, H1) every time the sample moves 1 nanometer.

[0046] S2200, controlling the shutter speed of the camera by external modulation so that the excitation signal period of the camera is equal to the sum of the motion period of the exciter and the preset phase difference.

[0047] It is understandable that in order to ensure that the camera can perform imaging sampling in accordance with the motion cycle of the vibrator, the shutter speed of the camera is controlled by external modulation so that the excitation signal cycle of the camera is synchronized with the motion cycle of the vibrator. Specifically, the excitation signal cycle of the camera is equal to the sum of the motion cycle of the vibrator and the preset phase difference, so that the shutter of the camera works synchronously with the vibrator, ensuring that the camera can completely collect the imaging data of the sample. Among them, controlling the shutter speed of the camera by external modulation belongs to the prior art and will not be repeated here.

[0048] S2300, sampling and imaging a preset area of ​​the sample according to the motion cycle of the vibrator.

[0049] It is understandable that after the exciter is turned on, the displacement step of the sample chamber is limited by the full-frame preview interface, and the movement speed is limited by the zoom speed. The camera shutter scanning speed also needs to adapt to the current zoom speed. Therefore, the standard mode sampling based on high-speed axial zoom technology has the function of full-frame preview, which is convenient for finding the characteristic surface and characteristic structure of the sample for sampling imaging.

[0050] S3000, when the required signal is fast imaging, the exciter is turned off, the camera adjusts the shutter speed according to the movement cycle of the sample chamber and samples and images the sample.

[0051] It is understandable that when the axial zoom technology is turned on, the full frame size is 2048*2048 pixels, and the amplitude of the exciter causes the relative movement between the light sheet and the camera imaging shutter. When the exciter is turned off, the frame size is 2048*180. This is because after the exciter is turned off, only the width of the light sheet can be imaged. Since the position of the light sheet is fixed, moving the sample chamber can achieve the result of changing the relative position of the light sheet and the sample, thereby realizing the optical tomography capability. However, in order to pursue higher resolution, the thickness of the light sheet is extremely thin and the imaging width is limited, so preview is not possible in this mode and the frame is small. The variable range of the camera acquisition speed is much greater than the sample chamber movement speed, so the acquisition speed of this acquisition mode is mainly limited by the sample chamber movement speed. However, at this time, the sampling speed is no longer limited by the zoom rate, but is mainly based on the sample chamber movement response rate and the camera shutter scanning rate.

[0052] It is understandable that the mode in which the exciter is turned off, the camera adjusts the shutter speed according to the movement cycle of the sample chamber and samples and images the sample is called the high-speed sampling mode. It can be seen that the imaging speed of the high-speed sampling mode is more than 20 times that of the standard sampling mode, which is more conducive to the actual high-throughput and high-efficiency large sample sampling and meets the needs of large-scale imaging in a short time.

[0053] See also Figure 4 , Figure 4 FIG. 5 is a schematic diagram showing a specific implementation process of another embodiment of the above step S3000. Figure 4 As shown, the imaging method based on high-speed axial zoom technology in the embodiment of the present application includes the following steps:

[0054] S3100, adjust the shutter speed so that the line scan rate of the camera is consistent with the movement frequency of the sample chamber.

[0055] It is understandable that in high-speed sampling mode, the camera's sampling speed is no longer limited by the zoom rate, but is mainly based on the sample chamber's movement response rate and the camera shutter scanning rate. Adjusting the shutter speed so that the camera's line scan rate is consistent with the sample chamber's movement frequency can ensure that the camera can cooperate with the sample chamber to collect imaging data.

[0056] It is understandable that in order to ensure that the camera can perform imaging sampling in accordance with the motion cycle of the exciter, the shutter speed of the camera is controlled by external modulation so that the excitation signal cycle of the camera is consistent with the movement frequency of the sample chamber. Among them, controlling the shutter speed of the camera by external modulation belongs to the prior art and will not be described in detail here.

[0057] S3200 performs sampling imaging on a preset area of ​​the sample according to the movement frequency of the sample chamber.

[0058] It can be understood that the camera sampling frequency is excited by an external square wave signal, which comes from the DAQ control and is consistent with the sample chamber movement frequency. Specifically, every time the sample chamber moves 1 nanometer, the camera captures an image according to the preset area (X2, Y2, W2, H2). At this time, the data acquisition speed is determined by the movement speed of the sample chamber.

[0059] See also Figure 5 , Figure 5 4 is a structural schematic diagram of a light sheet microscope imaging system 400 based on high-speed axial zoom technology provided in an embodiment of the present application. The entire process of the imaging method based on high-speed axial zoom technology provided in an embodiment of the present application involves the following modules in the light sheet microscope imaging system 400 based on high-speed axial zoom technology: an acquisition module 410, a first sampling module 420 and a second sampling module 430.

[0060] The acquisition module 410 is used to acquire the imaging requirement information of the sample and obtain the requirement signal;

[0061] The first sampling module 420 is used to start the vibrator and drive the reflector to perform axial scanning on the sample on the sample chamber when the demand signal is an imaging preview, and the camera adjusts the shutter speed according to the movement period of the vibrator and samples and images the sample;

[0062] The second sampling module 430 is used to turn off the exciter when the required signal is fast imaging, and the camera adjusts the shutter speed according to the movement cycle of the sample chamber and samples and images the sample.

[0063] It should be noted that the information interaction, execution process and other contents between the modules of the above-mentioned device are based on the same concept as the method embodiment of the present application. Their specific functions and technical effects can be found in the method embodiment part and will not be repeated here.

[0064] See also Figure 6 , Figure 6 4 is a schematic diagram of the structure of the first sampling module 420 and the second sampling module 430 in the light sheet microscope imaging system 400 based on high-speed axial zoom technology provided in an embodiment of the present application. The specific device position relationship of the first sampling module 420 and the second sampling module 430 and the technical effects brought about are as described in the above step S1300, and will not be repeated here.

[0065] Figure 7 The electronic device 500 provided in the embodiment of the present application is shown. The electronic device 500 includes but is not limited to:

[0066] Memory 501, used for storing programs;

[0067] The processor 502 is used to execute the program stored in the memory 501. When the processor 502 executes the program stored in the memory 501, the processor 502 is used to execute the above-mentioned imaging method based on the high-speed axial zoom technology.

[0068] The processor 502 and the memory 501 may be connected via a bus or other means.

[0069] The memory 501 is a non-transitory computer-readable storage medium that can be used to store non-transitory software programs and non-transitory computer executable programs, such as the imaging method based on high-speed axial zoom technology described in any embodiment of the present application. The processor 502 implements the above-mentioned imaging method based on high-speed axial zoom technology by running the non-transitory software program and instructions stored in the memory 501.

[0070] The memory 501 may include a program storage area and a data storage area, wherein the program storage area may store an operating system and application programs required for at least one function; the data storage area may store and execute the above-mentioned imaging method based on high-speed axial zoom technology. In addition, the memory 501 may include a high-speed random access memory, and may also include a non-volatile memory, such as at least one disk storage device, a flash memory device, or other non-volatile solid-state storage device. In some embodiments, the memory 501 may optionally include a memory remotely arranged relative to the processor 502, and these remote memories may be connected to the processor 502 via a network. Examples of the above-mentioned network include, but are not limited to, the Internet, an intranet, a local area network, a mobile communication network, and combinations thereof.

[0071] The non-transient software programs and instructions required to implement the above-mentioned imaging method based on high-speed axial zoom technology are stored in the memory 501. When executed by one or more processors 502, the imaging method based on high-speed axial zoom technology provided by any embodiment of the present application is executed.

[0072] The embodiment of the present application further provides a storage medium storing computer executable instructions, where the computer executable instructions are used to execute the above-mentioned imaging method based on high-speed axial zoom technology.

[0073] In one embodiment, the storage medium stores computer executable instructions, which are executed by one or more control processors 502, for example, by a processor 502 in the above-mentioned electronic device 500, so that the above-mentioned one or more processors 502 can execute the imaging method based on high-speed axial zoom technology provided in any embodiment of the present application.

[0074] The above described embodiments are merely illustrative, and the units described as separate components may or may not be physically separated, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the present embodiment.

[0075] It will be appreciated by those skilled in the art that all or some of the steps and systems in the disclosed method above may be implemented as software, firmware, hardware and appropriate combinations thereof. Some physical components or all physical components may be implemented as software executed by a processor, such as a central processing unit, a digital signal processor or a microprocessor, or may be implemented as hardware, or may be implemented as an integrated circuit, such as an application specific integrated circuit. Such software may be distributed on a computer-readable medium, which may include a computer storage medium (or a non-transitory medium) and a communication medium (or a temporary medium). As known to those skilled in the art, the term computer storage medium includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information (such as computer-readable instructions, data structures, program modules or other data). Computer storage media include, but are not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disks (DVD) or other optical disk storage, magnetic cassettes, magnetic tapes, disk storage or other magnetic storage devices, or any other medium that may be used to store desired information and may be accessed by a computer. Furthermore, it is well known to those skilled in the art that communication media typically include computer readable instructions, data structures, program modules, or other data in a modulated data signal such as a carrier wave or other transport mechanism, and may include any information delivery media.

Claims

1. An imaging method based on high-speed axial zoom technology, characterized in that: The method comprises: Acquire imaging requirement information of the sample and obtain a requirement signal; When the demand signal is an imaging preview, the vibrator is turned on, and the reflector is driven to perform axial scanning on the sample on the sample chamber, and the camera adjusts the shutter speed in accordance with the movement cycle of the vibrator and performs sampling and imaging on the sample; When the demand signal is for rapid imaging, the vibrator is turned off, and the camera adjusts the shutter speed in accordance with the movement cycle of the sample chamber and performs sampling and imaging of the sample.

2. The imaging method based on high-speed axial zoom technology according to claim 1, characterized in that: The step of acquiring imaging requirement information of the sample and obtaining a requirement signal includes: Acquiring imaging requirement information of the sample, including imaging area, scanning thickness and imaging speed of the sample; The demand signal is obtained according to the imaging area, the scanning thickness and the imaging speed.

3. The imaging method based on high-speed axial zoom technology according to claim 1, characterized in that: After obtaining the imaging requirement information of the sample and obtaining the requirement signal, the method further includes: The sample is placed on the sample chamber, wherein the sample chamber and the exciter are located on both sides of a polarization cube, and objective lenses are provided between the sample chamber and the exciter and the polarization cube, and the objective lenses form symmetrical light paths on both sides of the polarization cube.

4. The imaging method based on high-speed axial zoom technology according to claim 3, characterized in that: A lens group is also provided between the sample chamber and the polarization cube for correcting the spherical aberration and chromatic aberration generated by the reflector during its movement.

5. The imaging method based on high-speed axial zoom technology according to claim 1, characterized in that: The camera adjusts the shutter speed in accordance with the motion cycle of the vibrator and performs sampling imaging on the sample, including: Adjusting the shutter speed so that the line scan rate of the camera is consistent with the motion period of the vibrator; According to the motion cycle of the exciter, sampling and imaging are performed on a preset area of ​​the sample.

6. The imaging method based on high-speed axial zoom technology according to claim 5, characterized in that: The step of adjusting the shutter speed so that the line scan rate of the camera is consistent with the motion period of the vibrator includes: The shutter speed of the camera is controlled by external modulation so that the excitation signal period of the camera is equal to the sum of the movement period of the exciter and the preset phase difference.

7. The imaging method based on high-speed axial zoom technology according to claim 3, characterized in that: The camera adjusts the shutter speed in accordance with the movement cycle of the sample chamber and performs sampling and imaging of the sample, including: Adjusting the shutter speed so that the line scan rate of the camera is consistent with the movement frequency of the sample chamber; According to the moving frequency of the sample chamber, sampling and imaging are performed on a preset area of ​​the sample.

8. A light sheet microscope imaging system based on high-speed axial zoom technology, characterized in that: include: An acquisition module is used to acquire imaging requirement information of the sample and obtain a requirement signal; A first sampling module is used to start the vibrator and drive the reflector to perform axial scanning on the sample on the sample chamber when the demand signal is an imaging preview, and the camera adjusts the shutter speed in accordance with the movement cycle of the vibrator and performs sampling and imaging on the sample; The second sampling module is used to turn off the exciter when the required signal is fast imaging, and the camera adjusts the shutter speed according to the movement period of the sample chamber and samples and images the sample.

9. An electronic device, characterized in that: include: A memory, a processor, and a computer program stored in the memory and executable on the processor, wherein when the processor executes the computer program, the imaging method based on high-speed axial zoom technology as described in any one of claims 1 to 7 is implemented.

10. A computer-readable storage medium, characterized in that: A computer program is stored, and when the computer program is executed by a processor, an imaging method based on high-speed axial zoom technology as described in any one of claims 1 to 7 is implemented.

Images