Inoculation device for pleurotus nebrodensis liquid strain cultivation
By designing a liquid strain cultivation device of Bailingmush with a servo motor and gear system, the strain precipitation is prevented by shaking, and the strain growth is monitored in real time through the camera, the problem of difficulty in strain precipitation and growth monitoring in the existing technology is solved, and efficient strain cultivation and standardized production are achieved.
Patent Information
- Application Number
- CN202510329594.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-20
- Publication Date
- 2025-04-18
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing liquid strain cultivation device of white lingering mushrooms is prone to precipitation of strains during the cultivation process, resulting in strain failure to grow or die, and the growth rate of strains cannot be monitored in real time, and standardized production cannot be achieved.
A vaccination device for the cultivation of white lingmushroom liquid strains was designed. The gear system was driven by the servo motor to shake the culture dish, and the shock force generated by the shaking prevented bacterial strains from precipitating, and the growth of bacterial strains was monitored in real time through the camera and monitoring display screen.
It effectively prevents bacterial strain precipitation, improves the survival rate of bacterial strains, realizes the function of real-time monitoring of bacterial strain growth rate, and ensures the standardized production of bacterial strain cultivation.
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Figure CN119836982A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of Pleurotus nebrodensis liquid spawn cultivation, and in particular relates to an inoculation device for Pleurotus nebrodensis liquid spawn cultivation. Background Art
[0002] Pleurotus eryngii belongs to Fungi, Basidiomycetes, Agaricales, Pleurotus, and Pleurotus. It is also known as Asafoetida, Asafoetida, Asafoetida mushroom, white Asafoetida, and Asafoetida. Pleurotus eryngii is the trade name of palm-shaped Asafoetida. It is called Pleurotus eryngii because of its shape similar to Ganoderma lucidum and its pure white color. It is a wild and precious edible (medicinal) mushroom. "Liquid strain" is a liquid edible fungus strain produced by deep culture (liquid fermentation) technology in a biological fermentation tank using liquid culture medium. Liquid refers to the physical state of the culture medium, and liquid deep culture is the fermentation engineering technology. Inoculation devices are required for the cultivation of liquid strains of Pleurotus eryngii.
[0003] During the use of the existing inoculation device, a culture dish needs to be prepared. The liquid culture dish is heated and sterilized and then cooled. The liquid strain can be transplanted into the culture dish after sterilization is completed. The liquid strain can be directly injected for cultivation. However, during the cultivation period, the liquid strain is very likely to precipitate, causing the strain to not grow or even die. This does not meet the strain cultivation requirements, and the growth rate of the strain cannot be grasped in real time, and standardized production cannot be achieved. Therefore, we provide an inoculation device for the cultivation of white mushroom liquid strains. Summary of the invention
[0004] The object of the present invention is to provide an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis to solve the problems raised in the above-mentioned background technology.
[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, comprising a base box, a servo motor is fixedly installed on the top of the inner cavity of the base box, a first rotating rod is fixedly connected to the output end of the servo motor, one end of the first rotating rod is fixedly connected to a main gear, a sub-gear is meshed on the surface of the main gear, a second rotating rod is fixedly connected to the inner wall of the sub-gear, an inoculation box is fixedly connected to the top of the second rotating rod, a vertical plate is fixedly connected to the front end of the bottom of the inner cavity of the inoculation box, a group of cameras are arranged on the surface of the vertical plate, the surface of the inoculation box is movably connected to a box door through a hinge, the surface of the box door is fixedly connected to a monitoring display screen, and the camera and the monitoring display screen are electrically connected through a connecting line.
[0006] By adopting the above scheme, electrical energy is converted into mechanical energy through the servo motor, and the No. 1 rotating rod is driven to rotate, and the main gear is driven to rotate through the No. 1 rotating rod. Since the teeth on the surface of the main gear are meshed with the teeth on the surface of the sub-gear, when the main gear is in a rotating state, the sub-gear can be driven to rotate, and the No. 2 rotating rod is driven to rotate, and the No. 2 rotating rod is driven to rotate the inoculation box, and the inoculation box is driven to shake the culture dish, and the shock force generated by the shaking is used to prevent the bacteria inside the culture dish from precipitating, thereby greatly improving the survival rate of the bacteria.
[0007] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, a group of culture dishes are provided in the inner cavity of the inoculation box, and observation windows are provided on the surfaces of the culture dishes.
[0008] With the above solution, due to the particularity of the material of the observation window, it can be seen that there is no need to remove the sealing cover, and the bacterial species in the inner cavity of the culture dish can be checked with the cooperation of the camera.
[0009] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, the top of the right side of the culture dish is connected to a liquid inlet pipe, and one end of the liquid inlet pipe is threadedly connected to a sealing cap.
[0010] By adopting the above solution, one end of the liquid inlet pipe is sealed by setting the sealing cover, thereby effectively preventing external dust and moisture from entering the inner cavity of the culture dish through the liquid inlet pipe.
[0011] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, a sealing cover is provided on the top of the culture dish, one end of the sealing cover is fixedly connected to a connecting plate on the outside of the left side of the inoculation box, and one side of the connecting plate is fixedly connected to a handle.
[0012] By adopting the above scheme, the handle is held and pulled to one side, the connecting plate is driven to move by the handle, and the sealing cover is driven to move by the connecting plate until the sealing cover is pulled out from the top of the culture dish, so that the top of the culture dish is in an open state. Similarly, resetting the sealing cover can also make the culture dish in a sealed state to prevent the culture liquid from overflowing from the inner cavity of the culture dish during shaking.
[0013] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, mounting blocks are fixedly connected to both sides of the connecting plate, and the mounting blocks and the inoculation box are fixedly connected by mounting bolts.
[0014] By adopting the above scheme, the connecting plate is fixed by installing bolts to prevent the connecting plate from sliding during the rotation of the inoculation box, thereby improving the stability of the connecting plate when in use.
[0015] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, a bearing is movably connected to the bottom of the second rotating rod, and one end of the bearing is fixedly connected to the bottom of the inner cavity of the base box.
[0016] By adopting the above scheme, one end of the No. 2 rotating rod is limited by setting the bearing to prevent one end of the No. 2 rotating rod from being suspended in the air and then shaking.
[0017] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, a heating plate is fixedly connected to the back of the inoculation box, a temperature sensor is fixedly connected to the top of the inoculation box, a detection end of the temperature sensor is fixedly connected to the top of the inner cavity of the inoculation box with a detection head, a temperature display screen is fixedly connected to the left side of the top of the inoculation box, and the temperature sensor and the temperature display screen are electrically connected via a wire.
[0018] The above scheme is adopted to start the heating plate. When the heating plate is energized, under the action of the electric field, the carbon molecular clusters in the heating plate produce molecular motion, and violent friction and collision occur between the carbon atoms, thereby generating heat, thereby increasing the temperature of the inner cavity of the inoculation box. At the same time, the temperature is detected by the cooperation of the temperature sensor and the detection head, and the detected data is converted into an electrical signal and transmitted to the temperature display screen. The temperature is displayed on the temperature display screen for the user to view. When the temperature is too high or too low, the output power of the heating plate is increased or reduced through an external controller, thereby achieving the purpose of temperature control, so that the bacteria are always at a suitable temperature for growth.
[0019] As a preferred embodiment of an inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, a heat dissipation port is provided on the top of the left side of the base box, and a dustproof net is provided in the inner cavity of the heat dissipation port.
[0020] By adopting the above solution and setting the heat dissipation port, the heat of the servo motor during operation can be quickly discharged, thereby achieving the purpose of efficient heat dissipation.
[0021] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention converts electrical energy into mechanical energy through a servo motor, and drives the No. 1 rotating rod to rotate, and drives the main gear to rotate through the No. 1 rotating rod. Since the teeth on the surface of the main gear are meshed with the teeth on the surface of the sub-gear, when the main gear is in a rotating state, it can drive the sub-gear to rotate, and drive the No. 2 rotating rod to rotate, and drive the inoculation box to rotate through the No. 2 rotating rod, and drive the culture dish to shake through the inoculation box, and use the shock force generated by the shaking to prevent the bacteria inside the culture dish from precipitating, thereby greatly improving the survival rate of the bacteria.
[0022] The present invention uses a camera to shoot the liquid bacteria in the culture dish in real time, and converts the shooting picture into an electrical signal and transmits it to the monitoring display screen through a connecting line. The picture is displayed on the monitoring display screen. Subsequent users can view the growth rate of the bacteria in different time periods through the monitoring display screen and record it, thereby achieving the purpose of real-time monitoring. 3. The present invention starts the heating plate. When the heating plate is powered on, under the action of the electric field, the carbon molecular clusters in the heating plate produce molecular motion, and violent friction and collision occur between the carbon atoms, thereby generating heat, thereby increasing the temperature of the inner cavity of the inoculation box. At the same time, the temperature is detected by the cooperation of the temperature sensor and the detection head, and the detected data is converted into an electrical signal and transmitted to the temperature display screen. The temperature is displayed on the temperature display screen for the convenience of users to view. When the temperature is too high or too low, the output power of the heating plate is increased or decreased by an external controller, thereby achieving the purpose of temperature control, so that the bacteria are always at a suitable temperature for growth.
[0023] The present invention holds the handle and pulls it to one side, drives the connecting plate to move through the handle, and drives the sealing cover plate to move through the connecting plate until the sealing cover plate is pulled out from the top of the culture dish, so that the top of the culture dish is in an open state. Similarly, it can be known that resetting the sealing cover plate can also put the culture dish in a sealed state, preventing the culture liquid from overflowing from the inner cavity of the culture dish during shaking. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 It is a structural schematic diagram of the present invention; Figure 2 It is a cross-sectional view of the inoculation box of the present invention; Figure 3 is a schematic diagram of a vertical board of the present invention from a second viewing angle; Figure 4 It is a cross-sectional view of the base box of the present invention; Figure 5 It is a partial schematic diagram of the base box of the present invention from a second viewing angle; Figure 6 It is an enlarged view of point A of the present invention.
[0025] In the figure: 1. base box; 2. servo motor; 3. No. 1 rotating rod; 4. main gear; 5. sub-gear; 6. No. 2 rotating rod; 7. inoculation box; 8. culture dish; 9. observation window; 10. sealing cover plate; 11. connecting plate; 12. handle; 13. mounting block; 14. mounting bolts; 15. vertical plate; 16. camera; 17. box door; 18. monitoring display screen; 19. temperature sensor; 20. detection head; 21. temperature display screen; 22. heating plate. DETAILED DESCRIPTION
[0026] See also Figure 1-6An inoculation device for cultivating liquid spawn of Pleurotus eryngii comprises a base box 1, a servo motor 2 is fixedly installed on the top of the inner cavity of the base box 1, a first rotating rod 3 is fixedly connected to the output end of the servo motor 2, a main gear 4 is fixedly connected to one end of the first rotating rod 3, a sub-gear 5 is meshed on the surface of the main gear 4, a second rotating rod 6 is fixedly connected to the inner wall of the sub-gear 5, and an inoculation box 7 is fixedly connected to the top of the second rotating rod 6, see Figure 2 As shown, a heating plate 22 is fixedly connected to the back of the inoculation box 7, a temperature sensor 19 is fixedly connected to the top of the inoculation box 7, a detection head 20 is fixedly connected to the detection end of the temperature sensor 19 and located at the top of the inner cavity of the inoculation box 7, a temperature display screen 21 is fixedly connected to the left side of the top of the inoculation box 7, the temperature sensor 19 and the temperature display screen 21 are electrically connected through a wire, and the heating plate 22 is started. When the heating plate 22 is energized, under the action of the electric field, the carbon molecular clusters in the heating plate 22 produce molecular motion, and violent friction and collision occur between the carbon atoms, thereby generating heat, thereby increasing the temperature of the inner cavity of the inoculation box 7, and at the same time, through the matching of the temperature sensor 19 and the detection head 20 The temperature is detected and the detected data is converted into an electrical signal and transmitted to the temperature display screen 21. The temperature is displayed on the temperature display screen 21 for easy viewing by the user. When the temperature is too high or too low, the output power of the heating plate 22 is increased or decreased by the external controller, thereby achieving the purpose of temperature control so that the strains are always at a suitable temperature for growth. The front end of the bottom of the inner cavity of the inoculation box 7 is fixedly connected with a vertical plate 15, and a group of cameras 16 are arranged on the surface of the vertical plate 15. The surface of the inoculation box 7 is movably connected with a box door 17 through a hinge, and the surface of the box door 17 is fixedly connected with a monitoring display screen 18. The camera 16 and the monitoring display screen 18 are electrically connected through a connecting line. Figure 2 As shown, the inner cavity of the inoculation box 7 is provided with a group of culture dishes 8. Figure 5As shown, a sealing cover plate 10 is provided on the top of the culture dish 8, and a connecting plate 11 is fixedly connected to one end of the sealing cover plate 10 and located on the outside of the left side of the inoculation box 7, and a handle 12 is fixedly connected to one side of the connecting plate 11. The handle 12 is held and pulled to one side, and the connecting plate 11 is driven to move by the handle 12, and the sealing cover plate 10 is driven to move by the connecting plate 11 until the sealing cover plate 10 is pulled out from the top of the culture dish 8, so that the top of the culture dish 8 is in an open state. Similarly, resetting the sealing cover plate 10 can also make the culture dish 8 in a sealed state to prevent the culture liquid from overflowing from the inner cavity of the culture dish 8 during shaking. An observation window 9 is provided on the surface of the culture dish 8. Due to the particularity of the material of the observation window 9 It can be seen that there is no need to remove the sealing cover 10. With the cooperation of the camera 16, the bacteria in the inner cavity of the culture dish 8 can be checked. The servo motor 2 converts electrical energy into mechanical energy, and drives the No. 1 rotating rod 3 to rotate, and the No. 1 rotating rod 3 drives the main gear 4 to rotate. Because the teeth on the surface of the main gear 4 are meshed with the teeth on the surface of the sub-gear 5, when the main gear 4 is in a rotating state, it can drive the sub-gear 5 to rotate, and the sub-gear 5 drives the No. 2 rotating rod 6 to rotate, and the No. 2 rotating rod 6 drives the inoculation box 7 to rotate, and the inoculation box 7 drives the culture dish 8 to shake, and the shock force generated by the shaking is used to prevent the bacteria inside the culture dish 8 from precipitating, thereby greatly improving the survival rate of the bacteria.
[0027] See Figure 1 As shown, the top of the right side of the culture dish 8 is connected to a liquid inlet pipe, and one end of the liquid inlet pipe is threadedly connected to a sealing cover. Through the setting of the sealing cover, one end of the liquid inlet pipe is sealed to effectively prevent external dust and moisture from entering the inner cavity of the culture dish 8 through the liquid inlet pipe. Figure 6 As shown, both sides of the connecting plate 11 are fixedly connected with mounting blocks 13, and the mounting blocks 13 and the inoculation box 7 are fixedly connected by mounting bolts 14. The connecting plate 11 is fixed by the setting of the mounting bolts 14 to prevent the connecting plate 11 from sliding during the rotation of the inoculation box 7, thereby improving the stability of the connecting plate 11 when in use. Figure 4 As shown, the bottom of the second rotating rod 6 is movably connected with a bearing, and one end of the bearing is fixedly connected to the bottom of the inner cavity of the base box 1. Through the setting of the bearing, one end of the second rotating rod 6 is limited to prevent one end of the second rotating rod 6 from being suspended in the air and then shaking. Figure 4 As shown, a heat dissipation port is provided at the top of the left side of the base box 1, and a dustproof net is provided in the inner cavity of the heat dissipation port. Through the setting of the heat dissipation port, the heat of the servo motor 2 during operation can be quickly discharged to achieve the purpose of efficient heat dissipation.
[0028] When in use, first start the heating plate 22. When the heating plate 22 is energized, under the action of the electric field, the carbon molecular clusters in the heating plate 22 produce molecular motion, and violent friction and collision occur between the carbon atoms, thereby generating heat, thereby increasing the temperature of the inner cavity of the inoculation box 7, and sterilizing the culture dish 8 by high temperature. After the sterilization is completed, the culture liquid can be transported to the inner cavity of the culture dish 8 through the liquid inlet pipe after the culture dish 8 is cooled. After the transportation is completed, the user also transports the liquid bacteria to the inner cavity of the culture dish 8 through the liquid inlet pipe. When the liquid bacteria contact the culture liquid, the purpose of inoculation is achieved. During the cultivation period, the liquid bacteria in the culture dish 8 is photographed in real time through the camera 16 (since the camera 16 is an existing mature technology, it will not be described in detail here), and the photographed image is converted into an electrical signal and transmitted to the monitoring display screen 18 through a connecting line. The image is displayed on the monitoring display screen 18. The subsequent user can view the growth rate of the bacteria in different time periods through the monitoring display screen 18, and record it to achieve real-time monitoring. Purpose, at the same time, start the servo motor 2, convert electrical energy into mechanical energy through the servo motor 2, and drive the No. 1 rotating rod 3 to rotate, and drive the main gear 4 to rotate through the No. 1 rotating rod 3. Because the teeth on the surface of the main gear 4 are meshed with the teeth on the surface of the sub-gear 5, when the main gear 4 is in a rotating state, it can drive the sub-gear 5 to rotate, and drive the No. 2 rotating rod 6 to rotate through the sub-gear 5, and drive the inoculation box 7 to rotate through the No. 2 rotating rod 6, and drive the culture dish 8 to shake through the inoculation box 7. The shock force generated by the shaking is used to prevent the bacteria inside the culture dish 8 from precipitating, which greatly improves the survival rate of the bacteria. Finally, the temperature is detected through the cooperation of the temperature sensor 19 and the detection head 20, and the detected data is converted into an electrical signal and transmitted to the temperature display screen 21. The temperature is displayed on the temperature display screen 21 for the convenience of users to view. When the temperature is too high or too low, the output power of the heating plate 22 is increased or reduced through the external controller, thereby achieving the purpose of temperature control, so that the bacteria are always at a suitable temperature for growth.
Claims
1. An inoculation device for cultivating liquid spawn of Pleurotus nebrodensis, characterized in that: The invention comprises a base box (1), wherein a servo motor (2) is fixedly installed on the top of the inner cavity of the base box (1), the output end of the servo motor (2) is fixedly connected to a first rotating rod (3), one end of the first rotating rod (3) is fixedly connected to a main gear (4), the surface of the main gear (4) is meshed with a secondary gear (5), the inner wall of the secondary gear (5) is fixedly connected to a second rotating rod (6), the top of the second rotating rod (6) is fixedly connected to an inoculation box (7), the front end of the bottom of the inner cavity of the inoculation box (7) is fixedly connected to a vertical plate (15), the surface of the vertical plate (15) is provided with a group of cameras (16), the surface of the inoculation box (7) is movably connected to a box door (17) via a hinge, the surface of the box door (17) is fixedly connected to a monitoring display screen (18), and the camera (16) and the monitoring display screen (18) are electrically connected via a connecting line.
2. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 1, characterized in that: The inner cavity of the inoculation box (7) is provided with a group of culture dishes (8), and the surfaces of the culture dishes (8) are provided with observation windows (9).
3. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 2, characterized in that: The top of the right side of the culture dish (8) is connected to a liquid inlet pipe, and one end of the liquid inlet pipe is threadedly connected to a sealing cover.
4. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 2, characterized in that: A sealing cover plate (10) is provided on the top of the culture dish (8), and a connecting plate (11) is fixedly connected to one end of the sealing cover plate (10) and located on the outside of the left side of the inoculation box (7), and a handle (12) is fixedly connected to one side of the connecting plate (11).
5. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 4, characterized in that: Mounting blocks (13) are fixedly connected to both sides of the connecting plate (11), and the mounting blocks (13) and the inoculation box (7) are fixedly connected via mounting bolts (14).
6. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 1, characterized in that: The bottom of the second rotating rod (6) is movably connected to a bearing, and one end of the bearing is fixedly connected to the bottom of the inner cavity of the base box (1).
7. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 1, characterized in that: A heating plate (22) is fixedly connected to the back of the inoculation box (7), a temperature sensor (19) is fixedly connected to the top of the inoculation box (7), a detection head (20) is fixedly connected to the detection end of the temperature sensor (19) and located at the top of the inner cavity of the inoculation box (7), a temperature display screen (21) is fixedly connected to the left side of the top of the inoculation box (7), and the temperature sensor (19) and the temperature display screen (21) are electrically connected via a wire.
8. The inoculation device for cultivating liquid spawn of Pleurotus nebrodensis according to claim 1, characterized in that: A heat dissipation opening is provided at the top of the left side of the base box (1), and a dustproof net is provided in the inner cavity of the heat dissipation opening.