A pesticide composition for preventing and treating melon fruit fly

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating bitter gourd fruit fly. The pesticide composition has insecticidal activity and is formed by binary compounding of afoulanan and bromofenoximide or tetrazolyl amide. The mass ratio of the afoulanan to the bromofenoximide is 1-15:9-1. The mass ratio of the afoulanan to the tetrazolyl amide is 1-30:30-1. The pesticide composition of the present application is formed by compounding afoulanan and bromofenoximide or tetrazolyl amide with different action mechanisms, and the compounding combination shows synergistic effect on killing bitter gourd fruit fly, can improve the prevention and treatment effect, can reduce the amount of pesticide, can also be used for preparing stomach poison pesticide and toxic bait agent with high insecticidal activity on bitter gourd fruit fly, and has important significance for preventing and controlling bitter gourd fruit fly.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating melon fruit flies. BACKGROUND

[0002] The melon fruit fly (Bactrocera cucurbitae) is one of the important pests of melon and fruit crops. The loss degree of melon and fruit crops damaged by the melon fruit fly is generally more than 30%, and the damage to bitter melon is the most serious. In recent years, the pesticides for preventing and treating the melon fruit fly are excessively used in production, which causes serious pollution to the environment, and makes the melon fruit fly develop resistance to the pesticides to different degrees. Meanwhile, there is the problem of pesticide residues. With the improvement of people's ecological safety consciousness, the use of stomach poison pesticides to process toxic baits to prevent and treat the melon fruit fly gradually becomes the main means of the prevention and treatment strategy. Compared with contact pesticides, the toxic baits processed from stomach poison pesticides have the advantages of less use amount, high target specificity, safety to non-target organisms and less environmental pollution. Therefore, it is of great significance to screen stomach poison pesticides with high insecticidal activity against the melon fruit fly and process the stomach poison pesticides into toxic baits for preventing and treating the melon fruit fly.

[0003] Afoxolaner is a new isoxazoline insecticide and acaricide successfully developed by DuPont Company in the United States in 2008, CAS: 1093861-60-9, molecular formula: C 26 H 17 ClF9N3O3, relative molecular mass: 625.870, and the structural formula is as follows:

[0004]

[0005] Afoxolaner acts on the ligand-gated chloride channel, especially inhibits the channel gated by the neurotransmitter gamma-aminobutyric acid (GABA), blocks the transmission of chloride ions from the presynaptic membrane to the postsynaptic membrane, and causes the insect neurons to be excited excessively and die. The patent CN11838337A discloses a composition containing afoxolaner and cyantraniliprole, and discloses that the afoxolaner and cyantraniliprole have a synergistic effect when used for preventing and treating agricultural insects. There is no related report on the binary combination of afoxolaner and broflanectin or tetraniliprole. SUMMARY

[0006] The purpose of the present application is to provide a pesticide composition for preventing and treating melon fruit flies, so as to solve the problems caused by the excessive use of pesticides for preventing and treating melon fruit flies in production.

[0007] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0008] An insecticidal active pesticide composition, wherein the insecticidal active pesticide composition is a binary compound of afolanar and bromoxynil or tetrazolium; wherein the mass ratio of afolanar to bromoxynil is 1-15:9-1; and the mass ratio of afolanar to tetrazolium is 1-30:30-1.

[0009] For even better results, the mass ratio of afranal to bromfenac is 1:3.

[0010] For even better results, the mass ratio of afranal to tetrazolium is 1:7.

[0011] The present invention also provides the application of the aforementioned insecticidal active pesticide composition in the control of bitter gourd fruit fly.

[0012] The present invention also provides the application of the aforementioned insecticidal active pesticide composition in the preparation of a stomach poison or bait for bitter melon fruit fly.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The pesticide composition of the present invention combines afranal with bromoxynil or tetrazolium, which have different mechanisms of action. The combination exhibits a synergistic effect in killing bitter gourd fruit flies, which can improve the control effect and reduce the amount of pesticide used. It can also be used to prepare stomach poisons and poison baits with highly effective insecticidal activity against bitter gourd fruit flies, which is of great significance for the control of bitter gourd fruit flies. Detailed Implementation

[0015] The technical solution of this invention patent will be clearly and completely described below.

[0016] Examples Indoor bioactivity test

[0017] 1. Test pests

[0018] After harvesting the damaged bitter gourds, the melon fly larvae were picked out and reared for multiple generations on fresh, clean bitter gourds. Healthy adults that had emerged 3-4 days after hatching were then selected as test subjects.

[0019] Rearing conditions: Temperature 25±1℃, relative humidity 70±5%, photoperiod 16L / 8D.

[0020] 2. Test reagents

[0021] 98% afralanine technical grade, 98% brofenoxam technical grade, 90% tetrazolium technical grade.

[0022] 3. Test Methods

[0023] Liquid feeding method. The test drug was dissolved and diluted with 10% sucrose solution (solvent is 0.1% Tween-80 aqueous solution) to prepare a single-agent stock solution; multiple ratios were set up, and each single agent and each ratio mixture was diluted with 10% sucrose solution to prepare 5 mass concentration gradients for later use.

[0024] After sterilization, a 2cm×2cm×2cm sponge was completely immersed in the pesticide solution for 30 seconds, then removed. Once the solution stopped dripping, the sponge was placed in an Erlenmeyer flask. Twenty test pests, starved with water for 24 hours, were introduced into the flask, and the flask opening was sealed with gauze to prevent escape. The flasks were then kept at a temperature of 25±1℃, relative humidity of 70±5%, and a photoperiod of 16L / 8D, with each concentration repeated three times. A 10% sucrose solution treatment served as a blank control. The mortality rate of the test pests was observed 36 hours after treatment. The total number of pests and the number of dead pests for each treatment were recorded, and the corrected mortality rate for each treatment was calculated. The criterion for determining mortality was that the test pest could not turn over within 30 seconds of turning over.

[0025]

[0026] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0027]

[0028] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0029] 4. Data Analysis

[0030] Regression analysis was performed using DPS software on the logarithmic values ​​of drug concentrations and the corrected mortality rates for each treatment to calculate the LC ratio of each drug. 50 Then, the co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method.

[0031] 5. Drug efficacy evaluation

[0032] The synergistic effect of the agent was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The results are shown in Table 1-2.

[0033] Table 1. Toxicity test results of afranal combined with bromfenac on bitter melon fruit fly.

[0034]

[0035] Table 1 shows that the co-toxicity coefficients of afolanar and brofenoxam in a mass ratio range of 1-15:9-1 are all greater than 120, indicating that the combination of afolanar and brofenoxam exhibits a synergistic effect on the biological activity of the bitter gourd fruit fly. The synergistic effect is particularly pronounced at a mass ratio of 1:3, reaching 459.119.

[0036] Table 2. Toxicity test results of afranal combined with tetrazolium acetamiprid on bitter melon fruit fly.

[0037]

[0038]

[0039] Table 2 shows that the co-toxicity coefficients of afolanar and tetrazolium in a mass ratio range of 1-30:30-1 are all greater than 120, indicating that the combination of afolanar and tetrazolium exhibits a synergistic effect on the biological activity of the bitter gourd fruit fly. The synergistic effect is particularly significant at a mass ratio of 1:7, reaching 340.954.

[0040] In summary, the pesticide composition of the present invention combines afranal with bromoxynil or tetrazolium, which have different mechanisms of action. The combination exhibits a synergistic effect in killing bitter melon fly, which can improve the control effect and reduce the amount of pesticide used. It can also be used to prepare stomach poisons and poison baits with highly effective insecticidal activity against bitter melon fly, which is of great significance for the control of bitter melon fly.

Claims

1. An insecticidal active pesticide composition, characterized in that, The insecticidal active pesticide composition is a binary compound of afolanar and bromoxynil or tetrazolium; wherein the mass ratio of afolanar to bromoxynil is 1-15:9-1; and the mass ratio of afolanar to tetrazolium is 1-30:30-1.

2. The insecticidal active pesticide composition according to claim 1, characterized in that, The mass ratio of afranal to bromfenac is 1:

3.

3. The insecticidal active pesticide composition according to claim 1, characterized in that, The mass ratio of afranal to tetrazolium is 1:

7.

4. The application of the insecticidal active pesticide composition according to any one of claims 1-3 in the control of bitter gourd fruit fly.

5. The use of the insecticidal active pesticide composition according to any one of claims 1-3 in the preparation of a stomach poison or bait for bitter melon fruit fly.

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