Compound medicament for controlling termites based on serratia marcescens

Abstract

The invention provides a compound medicament for controlling termites based on serratia marcescens. The compound medicament comprises 7.75 * 10 < 8 >-2.63 * 10 < 12 > CFU / mL of serratia marcescens and pathogenic fungi or biological source pesticides or insect growth regulators or chemical agents, the pathogenic fungi are beauveria bassiana or metarhizium anisopliae; the biological source pesticide is ivermectin; the insect growth regulator is lufenuron or buprofezin or chlorfenapyroxad; the chemical agent is emamectin benzoate or indoxacarb. The compound medicament has an obvious synergistic effect on termite prevention and control, the use amount of a single medicament is reduced, and the compound medicament is lower in cost, more efficient, more stable and more environment-friendly.

Description

Technical Field

[0001] The invention belongs to the field of termite control, and particularly relates to a compound agent for controlling termites based on Serratia marcescens. Background Art

[0002] Termites are well-known social insects that usually feed on materials composed of cellulose, causing considerable damage to buildings, agricultural and forestry crops, standing trees and forests, reservoir dams, etc., causing significant losses to humans (Su and Scheffrahn., 2000; Wang et al., 2018., Chiu et al., 2022). Therefore, finding safe and efficient agents is a direction that needs to be considered for termite control. The Gram-negative bacterium Serratia marcesens is commonly found in insects raised in laboratories and collected in the wild, and has pathogenic effects on many insects including Orthoptera, Homoptera, Lepidoptera, etc. (Nehme et al. 2007., Niu et al. 2015., Aggarwal et al. 2017). Studies have shown that Serratia marcescens (SM1) has great application prospects in the biological control of pests (Bidari et al., 2017; Atanakul et al., 2020; Zhao et al., 2020). Our previous studies also showed that Serratia marcescens has a good control effect on black-winged termites (Fu et al., 2021; Wang et al. 2024). Therefore, the application of Serratia marcescens to control pests has great application prospects.

[0003] In addition to being used directly as biological agents to control pests, insect pathogens can also be used as biological enhancers of chemical pesticides or insect growth regulators. Insect pathogens, chemical pesticides, insect growth regulators, biopesticides and pathogenic fungi have different advantages, making their combined application a good way to solve many pest problems (Paula et al., 2011). Summary of the invention

[0004] The purpose of the invention is to obtain a more green and efficient compound agent for controlling termites by combining Serratia marcescens with different types of insecticides to control termites.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides a compound agent for controlling termites based on Serratia marcescens, wherein the compound agent composition comprises Serratia marcescens, and an insect growth regulator or pathogenic fungus or a biogenic pesticide or a chemical agent;

[0007] The insect growth regulator is lufenuron or buprofezin or hydrazine;

[0008] The pathogenic fungus is Beauveria bassiana or Metarhizium anisopliae;

[0009] The biogenic pesticide is ivermectin;

[0010] The chemical agent is ivermectin or emamectin benzoate or indoxacarb;

[0011] The bacterial count of Serratia marcescens was 7.75×10 8 ~2.63×10 12 CFU / mL;

[0012] The pathogenic fungus was 2×10 8 Conidia / mL;

[0013] The ivermectin is 0.01-0.05 mg / mL;

[0014] The concentration of the insect growth regulator is 1 to 5.11 mg / mL;

[0015] The concentration of the chemical agent is 0.01-0.02 mg / mL.

[0016] Furthermore, when the compound pharmaceutical composition is a compound of Serratia marcescens and thiamethoxam, the Serratia marcescens 7.75×10 8 ~8.75×10 10 CFU / mL, thiamethoxam is 1mg / ml~10 -2 mol / L means the concentration ratio is 1-3.05 mg / mL, and the compound volume ratio is 9:1-1:2.

[0017] When the compound pharmaceutical composition is Serratia marcescens and pathogenic fungi, the Serratia marcescens is 2.63×10 12 CFU / mL, pathogenic fungi are 2×10 8 Conidia / mL, the compound volume ratio is 2:1~1:2.

[0018] When the compound pharmaceutical composition is Serratia marcescens and hydrazine, the Serratia marcescens is 7.75×10 8 CFU / mL, the food inhibitor is 1 mg / ml, and the compound volume ratio is 5:1-1:9.

[0019] When the compound pharmaceutical composition is Serratia marcescens and lufenuron, the Serratia marcescens is 8.75×10 10 CFU / mL, lufenuron concentration is 10 -2mol / L (5.11 mg / mL), the compound volume ratio is 2:1 to 1:2.

[0020] When the compound pharmaceutical composition is Serratia marcescens, and emamectin benzoate or indoxacarb, the Serratia marcescens is 7.75×10 8 CFU / mL, the concentration of emamectin benzoate is 0.02 mg / mL, the concentration of indoxacarb is 0.01 mg / mL, and the volume ratio of the Serratia marcescens and emamectin benzoate or indoxacarb is 5:1 to 1:9.

[0021] When the compound medicine is 7.75×10 8 When CFU / mL of Serratia marcescens is mixed with 0.01 mg / mL of ivermectin, the volume ratio is 5:1 to 1:9.

[0022] When the compound medicine is 8.75×10 10 CFU / mL Serratia marcescens and 5.71×10 -5 When 1 mol / L (0.05 mg / mL) ivermectin is compounded, the volume ratio is 2:1 to 1:2.

[0023] Furthermore, the compounding volume ratio of the Serratia marcescens and thiamethoxam is 1:1.

[0024] Furthermore, the termites are black-winged termites or black-chested termites.

[0025] Beneficial effects of the present invention:

[0026] (1) By combining Serratia marcescens with other low-toxic and low-residue agents, the insecticide killing time can be shortened, the stability can be improved, the control effect can be enhanced, and the amount of insecticide used can be reduced, which is green, safe and pollution-free to the environment;

[0027] (2) The combined application of insect pathogenic bacteria and pathogenic fungi or biopesticides or insect growth regulators or chemical insecticides can increase their toxicity to pests by reducing the dosage of each other;

[0028] (3) The novel biological agent with enhanced toxicity of black-winged termites or black-chested termites provided by the present invention will provide new evidence for the compound preparation of insect pathogens and novel agents for controlling harmful insects, and also provide a new theoretical basis for the research and development of compound agents for controlling termites. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Corrected mortality for black-winged termites treated with a combination of SM1 and hydrazine.

[0030] Figure 2 The adjusted mortality rate of black-winged termites treated with a combination of SM1 and thiamethoxam.

[0031] Figure 3 The corrected mortality rate of black-winged termites treated with a combination of SM1 and emamectin benzoate.

[0032] Figure 4 The corrected mortality rate of black-winged termites treated with a combination of SM1 and indoxacarb.

[0033] Figure 5 The corrected mortality rate of black-winged termites treated with a combination of SM1 and ivermectin. DETAILED DESCRIPTION

[0034] 1. Materials and Methods

[0035] The test insects, black-chested Reticulitermes and black-winged soil termites, were collected from Nanjing, Jiangsu. The collected black-winged soil termites were placed in a device with a mushroom garden area separated from a feeding area, and raised at room temperature (25±1)°C, relative humidity 80%±5% RH, and 24h dark conditions.

[0036] The collected black-chested Reticulitermes were placed in a 20 cm × 8 cm × 5 cm plastic box for breeding, and the environmental conditions were darkness, temperature maintained at 25 ± 1 ° C, and relative humidity maintained at 90 ± 5%. The termites used in the test were uniformly sized and robust worker ants.

[0037] 2. Preparation of test bacteria and reagents

[0038] Serratia marcescens Bizio SM1 strain: isolated from infected termites that turned red after death, purified, and stored in an ultra-low temperature refrigerator at -80°C in this laboratory.

[0039] The SM1 strain stock solution was taken out from the -80℃ ultra-low temperature freezer and thawed. 1μL was added to 50mL of fermentation medium (peptone 10g / L + soybean oil 30g / L + NaCl 2g / L + K2HPO42g / L, pH 7.5) sterilized by high pressure steam (121℃ for 25min) and cultured by shaking. The culture conditions were: temperature 30℃, shaking table 200 rpm for 36h. The bacterial solution was prepared into a UV spectrophotometer to detect OD 600 When the concentration of SM1 solution is 0.6, stop shaking the bacteria and dilute it to 7.75×10 8 CFU / mL, 8.75×10 8 and 2.63×10 12 CFU / mL.

[0040] Beauveria bassiana (Bb) was cultured on potato dextrose agar (PDA) in an incubator at (25±1)°C and RH (75±5)% for 15 days. When the colony was producing spores, the culture medium was tilted, the hyphae and spores were gently scraped off with an inoculation needle, 0.05% Tween-80 sterile water was added, and the mother liquid was prepared by shaking and filtering. The number of spores per milliliter in the mother liquid was counted by a hemocytometer, and then the mother liquid was diluted to a concentration of 2×10 8 CFU / mL spore suspension.

[0041] The M. anisopliae (Ma) was cultured in LB medium and diluted to a concentration of 2×10 8 CFU / mL of spore suspension.

[0042] 91.4% RH-5849 was purchased from Jiangsu Yunnong Chemical Co., Ltd.; 98% buprofezin (BUP) was purchased from Shandong Huayang Pesticide Chemical Group Co., Ltd.; 74.2% emamectin benzoate (EMB) was purchased from Nanjing Red Sun Co., Ltd.; 93% indoxacarb (IND) was purchased from Jiangsu Jiannong Plant Protection Co., Ltd.; 50mg / 5g ivermectin (IVE) was purchased from Zhongnong Huawei (Hubei) Biopharmaceutical Co., Ltd.; 97.3% lufenuron was purchased from Shandong Luba Chemical Co., Ltd.; 97% buprofezin was purchased from Jiangsu Huifeng Shengmu Agriculture Co., Ltd. Each technical was dissolved in acetone to obtain 0.01mg / mL and 5.71×10 -5 mol / L ivermectin, 1 mg / mL and 1×10 -2 mol / L thiazinone, 1×10 -2 mol / L lufenuron, 1mg / mL hydrazine, 0.02mg / mL emamectin benzoate and 0.01mg / mL indoxacarb.

[0043] 3. Drug compounding and bioassay methods

[0044] 1. Enhanced toxicity of compound pesticides based on Serratia marcescens against black-winged termites

[0045] Take 540 μL of 7.75×10 8 CFU / mL SM1 bacterial solution and 60μL 1mg / mL hydrazine, 500μL 7.75×10 8 CFU / mL SM1 bacterial solution and 100μL 1mg / mL hydrazine, 300μL 7.75×10 8 CFU / mL SM1 bacterial solution and 300μL 1mg / mL hydrazine, 100μL 7.75×10 8 CFU / mL SM1 bacterial solution and 500μL 1mg / mL hydrazine, 60μL 7.75×10 8CFU / mL SM1 bacterial solution was mixed with 540μL 1mg / mL hydrazine to prepare compound drugs with volume ratios of 9:1, 5:1, 1:1, 1:5, and 1:9. The compound drugs of SM1 and buprofezin, indoxacarb, ivermectin, and emamectin benzoate were also prepared according to this method.

[0046] Place a qualitative filter paper with a diameter of 7 cm flat in a culture dish of appropriate size, take 600 μL of the compound agent and drip it evenly from the outer edge to the inside on the filter paper, spread it flat, make the filter paper fit the bottom of the dish without leaving any gaps, cover it after drying it slightly for use. Each dish is filled with 30 strong and similar-age black-winged termite nest workers outside the nest, and the test is carried out under dark conditions of (25±1)℃ and (80±5)%RH. Observe once every 12 hours. Individuals that cannot straighten their bodies and do not move after being lightly touched with a brush are recorded as dead. Pick out the dead workers and record the number of deaths. Continuously observe, pay attention to replenishing water in the filter paper during the observation, keep it moist, and use clean water as the water source until the end of the experiment. The compound agent is treated according to the above five compound ratios, and each treatment is repeated in 4 groups, and acetone is set as a control (the preliminary experimental results show that there is no difference between fermentation liquid and acetone as controls).

[0047] 2 Enhanced toxicity of a new compound biological agent based on Serratia marcescens against black-chested termites

[0048] Take 400 μL of 8.75×10 10 CFU / mL SM1 bacterial solution and 200μL 1×10 -2 mol / L lufenuron, 200μL8.75×10 10 CFU / mL SM1 bacterial solution and 200μL 1×10 -2 mol / L lufenuron, 200μL 8.75×10 10 CFU / mL SM1 bacterial solution and 400μL 1×10 -2 mol / L lufenuron, mix thoroughly, and prepare compound agents with volume ratios of 2:1, 1:1, and 1:2 respectively. The compound agents of SM1 with buprofezin, ivermectin, Beauveria bassiana, and Metarhizium anisopliae were also prepared according to this method.

[0049] The bioassay was performed using the insect immersion method. Cut the filter paper into the size of a petri dish, lay it flat in the dish, soak the filter paper with water, and select healthy, vigorous, and similar-sized black-chested termites for the experiment. Immerse the black-chested termites in the droplet for 10 seconds, then immediately use a brush to pick the treated termites back into the dish, place the petri dish in the dark, observe regularly, and record the mortality rate of the black-chested termites. Each single agent and each ratio of the compound reagent were set up with 3 replicates, and the control was also set up with 3 replicates.

[0050] Statistical Analysis

[0051] The mortality rate of termites was corrected using the Abbott formula (Abbott., 1925): Corrected mortality rate = (TC) / (1-C)×100; T represents the mortality rate of the treatment group, and C represents the mortality rate of the control group.

[0052] The experimental data were processed using the DPS data processing system (Tang and Zhang, 2013). The professional statistics-biostatistics and pharmacokinetics-mass reaction bioassay probability value analysis function of the software was used to analyze the median lethal time (LT) of the biopesticide SM1 mixed with other agents. 50 . The collected data were subjected to analysis of variance (ANOVA) using InStat software (GraphPad, San Diego, CA, USA). One-way ANOVA was used to evaluate the significance between multiple samples, and then Tukey's test was used for comparative analysis, with the significance level set at P < 0.05. The co-toxicity coefficient method (CTC) (Sun and Johnson., 1960) was used to analyze the combined toxicity of SM1 mixed with various agents to termites:

[0053] The TI of a standard potion is 100

[0054] Toxicity index (TI) = (LT of standard insecticide 50 / LT for single dose tested 50 )×100

[0055] Actual Toxicity Index (ATI) = (LT of standard insecticide) 50 LT / Mixture 50 )×100

[0056] Theoretical toxicity index (TTI) of a mixture = (toxicity index TI of a single dose of agent A × the percentage of agent A in the mixture + the toxicity index TI of a single dose of agent B × the percentage of agent B in the mixture) × 100.

[0057] Co-toxicity coefficient (CTC) = actual toxicity index ATI / theoretical toxicity index TTI of mixture × 100

[0058] The synergistic effect was determined by the size of the CTC value: CTC < 80 was an antagonistic effect; 80 ≤ CTC ≤ 120 was an additive effect; CTC > 120 was a synergistic effect (Sun and Johnson., 1960).

[0059] V. Results Analysis

[0060] 1 Toxicity test of the combination of Serratia marcescens SM1 and hydrazine (RH-5849) against Coptotermes nigropteroides

[0061] from Figure 1 It can be seen that 7.75×10 8 The adjusted mortality rate of the treatment group with a volume ratio of CFU / mL SM1 to 1 mg / mL anorectic hydrazine of 1:5 was significantly higher than that of the single-dose treatment group; 7.75×10 8 The corrected mortality of black-winged termites treated with the combination of CFU / mL SM1 and 1 mg / mL hydrazine at a volume ratio of 1:1, 1:5, and 1:9 showed significant differences compared with the single-dose treatment group; and 7.75×10 8 The adjusted mortality rate of the group treated with a volume ratio of 9:1 of CFU / mL SM1 to 1 mg / mL anorectic hydrazine was lower than that of the single-dose treatment group at all time periods.

[0062] From Table 1, we can see that 7.75×10 8 The LT50 of CFU / mL SM1 against black-winged termites is 74.26h; the LT50 of 1mg / mL hydrazine against black-winged termites is 50 54.98h. 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a volume ratio of 9:1 to LT of black-winged termites. 50 63.24h; 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a volume ratio of 5:1 to LT of black-winged termites. 50 45.13h; 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a 1:1 volume ratio to LT of black-winged termites. 50 31.37h; 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed at a volume ratio of 1:5 to LT of black-winged termites. 50 24.52h; 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a volume ratio of 1:9 to LT of black-winged termites. 50 It is 38.01h.

[0063] Table 1 Toxicity test of SM1 and hydrazine against black-winged termites

[0064]

[0065] Table 2 Co-toxicity coefficient of SM1 and hydrazine to black-winged termites

[0066]

[0067] TI, ATI, TTI and CTC represent toxicity index, actual toxicity index, theoretical toxicity index and co-toxicity coefficient, respectively.

[0068] According to Table 2, the toxicity index of hydrazine is 135.07 when SM1 is used as the standard agent. 8 When CFU / mLSM1 and 1mg / mL hydrazine were mixed at a volume ratio of 9:1, the actual toxicity index of the mixture was 117.43, the theoretical toxicity index was 103.51, and the co-toxicity coefficient was 113.45, which was less than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a volume ratio of 9:1, which showed additive toxicity to black-winged termites. 8 When CFU / mL SM1 and 1 mg / mL hydrazine were mixed at a volume ratio of 5:1, the actual toxicity index of the mixture was 164.55, the theoretical toxicity index was 105.84, and the co-toxicity coefficient was 155.46, which was greater than 120. Therefore, 7.75×10 8 When SM1 and 1 mg / mL hydrazine were mixed in a volume ratio of 5:1, they had a synergistic toxicity against black-winged termites. When SM1 and 1 mg / mL hydrazine were mixed in a volume ratio of 1:1, the actual toxicity index of the mixture was 236.72, the theoretical toxicity index was 117.53, and the co-toxicity coefficient was 201.41, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a 1:1 volume ratio and showed enhanced toxicity against black-winged termites. 8 When CFU / mL SM1 and 1mg / mL hydrazine were mixed at a volume ratio of 1:5, the actual toxicity index of the mixture was 302.85, the theoretical toxicity index was 129.22, and the co-toxicity coefficient was 234.37, which was much greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 1mg / mL hydrazine were mixed in a volume ratio of 1:5, which had a synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 1mg / mL hydrazine were mixed at a volume ratio of 1:9, the actual toxicity index of the mixture was 195.37, the theoretical toxicity index was 131.56, and the co-toxicity coefficient was 148.50, which was greater than 120. Therefore, 7.75×10 8 When CFU / mL SM1 and 1 mg / mL hydrazine were mixed in a volume ratio of 1:9, they had a synergistic toxicity against black-winged termites.

[0069] 2 Toxicity determination of the combination of Serratia marcescens SM1 and buprofezin (BUP) against Coptotermes nigricans

[0070] from Figure 2 It can be seen that 7.75×10 8 CFU / mL SM1 and 1mg / mL buprofezin single agent and each mixture after treatment of black-winged termites at 24h, 7.75×10 8 The adjusted mortality rate of the group treated with a 1:1 volume ratio of CFU / mL SM1 and 1 mg / m thiazide was significantly different from that of the other groups; 7.75×10 8 There were differences in the adjusted mortality rates between the combined drug treatment groups with a volume ratio of CFU / mL SM1 to 1 mg / mL thiazide of 9:1, 5:1 and 1:1 and the single-dose treatment group, and the adjusted mortality rates of the treatment groups with a ratio of 1:5 and 1:9 were lower during the entire experimental period.

[0071] Table 3 Toxicity test of SM1 and buprofezin against black-winged termites

[0072]

[0073] Table 4 Co-toxicity coefficient of SM1 and thiamethoxam to black-winged termites

[0074]

[0075] From Table 3, we can see that 7.75×10 8 CFU / mL SM1 against black-winged termites LT 50 The LT of 1 mg / mL thiamethoxam to black-winged termites is 74.26 h. 50 92.50h. 7.75×10 8 CFU / mL SM1 and 1mg / mL buprofezin were mixed in a volume ratio of 9:1 to LT of black-winged termites. 50 56.12h; 7.75×10 8 CFU / mL SM1 and 1mg / mL buprofezin were mixed in a volume ratio of 5:1 to LT of black-winged termites. 50 48.12h; 7.75×10 8 CFU / mL SM1 and 1mg / mL buprofezin were mixed in a 1:1 volume ratio to LT against black-winged termites. 50 45.42h; 7.75×10 8 CFU / mL SM1 and 1mg / mL buprofezin were mixed at a volume ratio of 1:5 to LT of black-winged termites. 50 111.97h; 7.75×10 8 CFU / mL SM1 and 1mg / mL thiamethoxam were mixed in a volume ratio of 1:9 to LT of black-winged termites. 50 It is 90.26h.

[0076] According to Table 4, the toxicity index of thiamethoxam is 80.28 when SM1 is used as the standard agent. 8 When CFU / mLSM1 and 1mg / mL buprofezin were mixed at a volume ratio of 9:1, the actual toxicity index of the mixture was 132.34, the theoretical toxicity index was 98.03, and the co-toxicity coefficient was 135.00, which was greater than 120. Therefore, 7.75×10 8 When SM1 and 1 mg / mL thiamethoxam were mixed in a volume ratio of 9:1, they had a synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 1 mg / mL buprofezin were mixed at a volume ratio of 5:1, the actual toxicity index of the mixture was 154.32, the theoretical toxicity index was 96.71, and the co-toxicity coefficient was 159.57, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 1mg / mL thiamethoxam in a volume ratio of 5:1 have synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 1 mg / mL buprofezin were mixed at a volume ratio of 1:1, the actual toxicity index of the mixture was 163.50, the theoretical toxicity index was 90.14, and the co-toxicity coefficient was 181.38, which was greater than 120. Therefore, 7.75×10 8 When SM1 and 1mg / mL thiamethoxam were mixed in a volume ratio of 1:1, they had a synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 1 mg / mL buprofezin were mixed at a volume ratio of 1:5, the actual toxicity index of the mixture was 66.32, the theoretical toxicity index was 83.57, and the co-toxicity coefficient was 79.36, which was less than 80. Therefore, 7.75×10 8 When SM1 and 1mg / mL thiamethoxam were mixed in a volume ratio of 1:5, they had antagonistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 1 mg / mL buprofezin were mixed at a volume ratio of 1:9, the actual toxicity index of the mixture was 82.27, the theoretical toxicity index was 82.25, and the co-toxicity coefficient was 100.02, which was less than 120. Therefore, 7.75×10 8 When CFU / mL SM1 and 1 mg / mL thiamethoxam were mixed in a volume ratio of 1:9, they had additive toxicity to black-winged termites.

[0077] 3 Toxicity determination of the combination of Serratia marcescens SM1 and emamectin benzoate (EMB) against Coptotermes nigropteroides

[0078] from Figure 3 It can be seen that 7.75×10 8There was no significant difference in the corrected mortality of black-winged termites treated with SM1 and 0.02 mg / mL emamectin benzoate alone or in the mixture within 24 h. At 48 h, 7.75×10 8 The corrected mortality rate of black-winged termites treated with a combination of CFU / mL SM1 and 0.02 mg / mL emamectin benzoate in a volume ratio of 5:1 showed significant differences from that of the single-agent treatment group, and there were significant differences between the combination treatment groups and the single-agent treatment groups between 72h and 96h.

[0079] Table 5 Toxicity test of SM1 and emamectin benzoate complex to black-winged termite

[0080]

[0081] Table 6 Co-toxicity coefficient of SM1 and emamectin benzoate on black-winged termites

[0082]

[0083] From Table 5, we can see that 7.75×10 8 CFU / mL SM1 against black-winged termites LT 50 The LT of 0.02 mg / mL emamectin benzoate against black-winged termites is 74.26 h. 50 is 105.21h. 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate were mixed in a volume ratio of 9:1 to LT of black-winged termites. 50 42.17h; 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate were mixed in a volume ratio of 5:1 to LT of black-winged termites. 50 48.36h; 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate were mixed in a 1:1 volume ratio to LT of black-winged termites. 50 48.77h; 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate were mixed in a volume ratio of 1:5 to LT of black-winged termites. 50 57.03h; 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate were mixed in a volume ratio of 1:9 to LT of black-winged termites. 50 It is 45.56h.

[0084] According to Table 6, taking SM1 as the standard agent, the toxicity index of emamectin benzoate is 70.58. 8When CFU / mLSM1 and 0.02mg / mL emamectin benzoate were mixed at a volume ratio of 9:1, the actual toxicity index of the mixture was 176.10, the theoretical toxicity index was 97.06, and the co-toxicity coefficient was 181.43, which was greater than 120. Therefore, 7.75×10 8 When SM1 and 0.02 mg / mL emamectin benzoate were mixed in a volume ratio of 9:1, they had enhanced toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.02 mg / mL emamectin benzoate were mixed at a volume ratio of 5:1, the actual toxicity index of the mixture was 153.56, the theoretical toxicity index was 95.10, and the co-toxicity coefficient was 161.47, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate in a volume ratio of 5:1 have synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.02 mg / mL emamectin benzoate were mixed in a 1:1 volume ratio, the actual toxicity index of the mixture was 152.27, the theoretical toxicity index was 85.29, and the co-toxicity coefficient was 178.52, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.02mg / mL emamectin benzoate in a 1:1 volume ratio have synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.02 mg / mL emamectin benzoate were mixed at a volume ratio of 1:5, the actual toxicity index of the mixture was 130.21, the theoretical toxicity index was 75.49, and the co-toxicity coefficient was 172.50, which was greater than 120. Therefore, 7.75×10 8 When CFU / mLSM1 and 0.02mg / mL emamectin benzoate were mixed in a volume ratio of 1:5, it had a synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.02 mg / mL emamectin benzoate were mixed at a volume ratio of 1:9, the actual toxicity index of the mixture was 162.99, the theoretical toxicity index was 73.52, and the co-toxicity coefficient was 221.69, which was much greater than 120. Therefore, 7.75×10 8 When CFU / mL SM1 and 0.02mg / mL emamectin benzoate are mixed in a volume ratio of 1:9, they have a synergistic toxicity against black-winged termites.

[0085] 4 Toxicity determination of the combination of Serratia marcescens SM1 and indoxacarb (IND) against Coptotermes nigropteroides

[0086] from Figure 4 It can be seen that 7.75×10 8There was no significant difference in CFU / mL of SM1 and 0.01 mg / mL indoxacarb alone and in the mixtures at 24 h. 8 There was a significant difference in the adjusted mortality between the combined treatment groups of SM1 and 0.01 mg / mL indoxacarb at a volume ratio of 1:1, 1:5 and 1:9 and the single-dose treatment group. 8 The adjusted mortality rate of the group treated with CFU / mL SM1 and 0.01 mg / mL indoxacarb at a volume ratio of 9:1 was lower than that of the single-dose treatment group throughout the experiment.

[0087] From Table 7, we can see that 7.75×10 8 CFU / mL SM1 against black-winged termites LT 50 The LT of 0.01mg / mL indoxacarb against black-winged termites is 74.26h. 50 64.89h. 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed in a volume ratio of 9:1 to kill LT of black-winged termites. 50 82.57h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed in a volume ratio of 5:1 to LT of black-winged termites. 50 58.57h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed in a 1:1 volume ratio to LT against black-winged termites. 50 43.65h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed at a volume ratio of 1:5 to LT of black-winged termites. 50 42.79h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed in a volume ratio of 1:9 to LT of black-winged termites. 50 It is 39.00h.

[0088] Table 7 Toxicity test of SM1 and indoxacarb against black-winged termites

[0089]

[0090] According to Table 8, taking SM1 as the standard agent, the toxicity index of indoxacarb is 114.44. 8When CFU / mLSM1 and 0.01mg / mL indoxacarb were mixed at a volume ratio of 9:1, the actual toxicity index of the mixture was 89.94, the theoretical toxicity index was 101.44, and the co-toxicity coefficient was 88.66, which was less than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb in a volume ratio of 9:1 have additive toxicity to black-winged termites. 8 When CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed at a volume ratio of 5:1, the actual toxicity index of the mixture was 126.79, the theoretical toxicity index was 102.41, and the co-toxicity coefficient was 123.81, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb in a volume ratio of 5:1 have synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01 mg / mL indoxacarb were mixed at a volume ratio of 1:1, the actual toxicity index of the mixture was 170.13, the theoretical toxicity index was 107.22, and the co-toxicity coefficient was 158.67, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb in a 1:1 volume ratio have enhanced toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01 mg / mL indoxacarb were mixed at a volume ratio of 1:5, the actual toxicity index of the mixture was 173.55, the theoretical toxicity index was 112.03, and the co-toxicity coefficient was 154.91, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL indoxacarb in a volume ratio of 1:5 have synergistic toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed at a volume ratio of 1:9, the actual toxicity index of the mixture was 190.41, the theoretical toxicity index was 113.00, and the co-toxicity coefficient was 168.51, which was greater than 120. Therefore, 7.75×10 8 When CFU / mL SM1 and 0.01mg / mL indoxacarb were mixed in a volume ratio of 1:9, it had a synergistic toxicity against black-winged termites.

[0091] Table 8 Co-toxicity coefficient of SM1 and indoxacarb against black-winged termites

[0092]

[0093] Toxicity determination of the combination of Serratia marcescens SM1 and ivermectin (IVE) against Coptotermes pygmaea

[0094] Depend on Figure 5 It can be seen that 7.75×10 8 There was no significant difference in the corrected mortality of C. sphaerocephalus treated with single agent SM1 and 0.01 mg / mL ivermectin and the mixture within 24 h; at 48 h and 72 h, 7.75 × 10 8 There was a significant difference in the adjusted mortality between the combined treatment group with a volume ratio of 1:9 of SM1 and 0.01 mg / mL ivermectin and the single-dose treatment group; 7.75×10 8 There was a significant difference in the adjusted mortality rate between the combination treatment groups with a volume ratio of 5:1 and 1:9 of CFU / mL SM1 and 0.01 mg / mL ivermectin and the single-dose treatment group.

[0095] From Table 9, we can see that 7.75×10 8 CFU / mL SM1 against black-winged termites LT 50 The LT of 0.01 mg / mL ivermectin against black-winged termites is 74.26 h. 50 74.66h. 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin were mixed in a volume ratio of 9:1 to LT against black-winged termites. 50 65.11h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin were mixed in a volume ratio of 5:1 to LT against black-winged termites. 50 59.98h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin were mixed in a 1:1 volume ratio to LT against black-winged termites. 50 77.63h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin were mixed in a volume ratio of 1:5 to LT against black-winged termites. 50 58.19h; 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin were mixed in a volume ratio of 1:9 to LT against black-winged termites. 50 It is 39.68h.

[0096] According to Table 10, the toxicity index of ivermectin is 99.46 when SM1 is used as the standard agent. 8 When CFU / mL SM1 and 0.01 mg / mL ivermectin were mixed at a volume ratio of 9:1, the actual toxicity index of the mixture was 114.05, the theoretical toxicity index was 99.95, and the co-toxicity coefficient was 114.11, which was less than 120. Therefore, 7.75×108 CFU / mL SM1 and 0.01mg / mL ivermectin in a volume ratio of 9:1 have additive toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01mg / mL ivermectin were mixed at a volume ratio of 5:1, the actual toxicity index of the mixture was 123.81, the theoretical toxicity index was 99.91, and the co-toxicity coefficient was 123.92, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin in a volume ratio of 5:1 have enhanced toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01 mg / mL ivermectin were mixed at a volume ratio of 1:1, the actual toxicity index of the mixture was 95.66, the theoretical toxicity index was 99.73, and the co-toxicity coefficient was 95.92, which was less than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin in a 1:1 volume ratio have additive toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01 mg / mL ivermectin were mixed at a volume ratio of 1:5, the actual toxicity index of the mixture was 127.62, the theoretical toxicity index was 99.55, and the co-toxicity coefficient was 128.19, which was greater than 120. Therefore, 7.75×10 8 CFU / mL SM1 and 0.01mg / mL ivermectin in a volume ratio of 1:5 have enhanced toxicity against black-winged termites. 8 When CFU / mL SM1 and 0.01mg / mL ivermectin were mixed at a volume ratio of 1:9, the actual toxicity index of the mixture was 187.15, the theoretical toxicity index was 99.52, and the co-toxicity coefficient was 188.05, which was much greater than 120. Therefore, 7.75×10 8 When CFU / mL SM1 and 0.01 mg / mL ivermectin are mixed in a volume ratio of 1:9, they have synergistic toxicity against black-winged termites.

[0097] Table 9 Toxicity determination of SM1 and ivermectin to black-winged termites

[0098]

[0099]

[0100] Table 10 Co-toxicity coefficient of SM1 and ivermectin combined with black-winged termites

[0101]

[0102] 6 Toxicity determination of the complex of Serratia marcescens SM1 and lufenuron against Reticulitermes nigromaculata

[0103] From Table 11, we can see that the concentration is 1×10 -2 mol / L Lufenuron against black-chested termites 50 160.45h; 8.75×10 10 CFU / mL SM1 against black-chested termites LT 50 206.21h. 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L lufenuron was mixed with black-chested termite LT in a volume ratio of 2:1 50 185.30h; 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L lufenuron was mixed with black-chested termite LT in a volume ratio of 1:1 50 113.62h; 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L lufenuron was mixed with black-chested termite LT in a volume ratio of 1:2 50 It is 86.49h.

[0104] Table 11 Toxicity determination of SM1 and lufenuron to black-chested termites

[0105]

[0106] Table 12 Co-toxicity coefficient of SM1 and lufenuron to black-chested termites

[0107]

[0108] According to Table 12, the toxicity index of SM1 is 77.80 when lufenuron is used as the standard agent. 10 CFU / mLSM1 and 1×10 -2 When 10 mol / L lufenuron was mixed at a volume ratio of 2:1, the actual toxicity index of the mixture was 111.24, the theoretical toxicity index was 85.20, and the calculated toxicity co-toxicity coefficient was 130.56, which was greater than 120, indicating that 8.75×10 10 CFU / mL SM1 and 1×10 - 2 When 8.75×10 10 CFU / mLSM1 and 1×10 -2When 1 mol / L lufenuron was mixed at a volume ratio of 1:1, the actual toxicity index of the mixture was 141.20, the theoretical toxicity index was 88.90, and the calculated toxicity co-toxicity coefficient was 158.82, which was much larger than 120, indicating that 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L lufenuron in a 1:1 volume ratio had a synergistic effect on the toxicity of black-chested termites. 10 CFU / mLSM1 and 1×10 -2 When 1 mol / L lufenuron was mixed at a volume ratio of 1:2, the actual toxicity index of the mixture was 131.36, the theoretical toxicity index was 92.60, and the calculated toxicity co-toxicity coefficient was 141.86, which was much larger than 120, indicating that 8.75×10 10 CFU / mL SM1 and 1×10 -2 The toxicity of 1 mol / L lufenuron to Reticulitermes nigra was enhanced in a volume ratio of 1:2.

[0109] 7 Toxicity determination of the combination of Serratia marcescens SM1 and buprofezin against Reticulitermes nigricans

[0110] From Table 13, we can see that 1×10 -2 mol / L thiamethoxam to the LT of black-chested termites 50 179.99h; 8.75×10 10 CFU / mL SM1 against black-chested termites LT 50 206.21h. 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L thiamethoxam was mixed with black-chested termite LT at a volume ratio of 2:1 50 100.03h; 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L thiamethoxam was mixed with black-chested termite LT in a volume ratio of 1:1 50 85.03h; 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L thiamethoxam was mixed with black-chested termite LT in a volume ratio of 1:2 50 It is 71.26h.

[0111] Table 13 Toxicity determination of SM1 and buprofezin to black-chested termites

[0112]

[0113] Table 14 Co-toxicity coefficient of SM1 and thiamethoxam to black-chested termites

[0114]

[0115] According to Table 14, the toxicity index of SM1 is 87.28 when 8.75×10 10 CFU / mLSM1 and 1×10 -2 When 1 mol / L thiamethoxam was compounded at a volume ratio of 2:1, the actual toxicity index of the mixture was 206.13, the theoretical toxicity index was 91.52, and the calculated toxicity co-toxicity coefficient was 225.23, which was much larger than 120, indicating that 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L thiamethoxam in a volume ratio of 2:1 has a synergistic effect on the toxicity of black-chested termites. 10 CFU / mLSM1 and 1×10 -2 When 1 mol / L thiamethoxam was compounded at a volume ratio of 1:1, the actual toxicity index of the mixture was 211.68, the theoretical toxicity index was 93.64, and the calculated toxicity co-toxicity coefficient was 226.05, which was much larger than 120, indicating that 8.75×10 10 CFU / mL SM1 and 1×10 -2 mol / L thiamethoxam in a 1:1 volume ratio had a synergistic effect on the toxicity of black-chested termites. 10 CFU / mLSM1 and 1×10 -2 When 1 mol / L thiamethoxam was compounded at a volume ratio of 1:2, the actual toxicity index of the mixture was 119.32, the theoretical toxicity index was 95.76, and the calculated toxicity co-toxicity coefficient was 124.60, which was greater than 120, indicating that 8.75×10 10 CFU / mL SM1 and 1×10 - 2 The toxicity of 1 mol / L thiamethoxam to Reticulitermes nigra was enhanced by the compound of 1:2 volume ratio.

[0116] 8 Toxicity test of the combination of Serratia marcescens SM1 and Beauveria bassiana Bb against Reticulitermes nigropunctatus

[0117] From Table 15, we can see that 2.63×10 12 CFU / mL SM1 against black-chested termites LT 50 16.71h; 2×10 8 LT of Conidia / mL Bb against Reticulitermes fuscipiens 50 16.56h. 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Bb was mixed at a volume ratio of 2:1 to LT of black-chested termites.50 9.93h; 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Bb was mixed in a volume ratio of 1:1 to LT of black-chested termites. 50 8.53h; 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Bb was mixed at a volume ratio of 1:2 to LT of black-chested termites. 50 It is 7.20h.

[0118] Table 15 Toxicity determination of SM1 and Bb against black-chested termites

[0119]

[0120] According to Table 16, taking SM1 as the standard agent, the toxicity index of Bb is 100.88. 12 CFU / mL SM1 and 2×10 8 When Conidia / mL Bb was mixed at a volume ratio of 2:1, the actual toxicity index of the mixture was 168.20, the theoretical toxicity index was 100.29, and the co-toxicity coefficient was 167.71, which was much greater than 120. Therefore, 2.63×10 12 CFU / mL SM1 and 2×10 8 When Conidia / mL Bb was mixed at a volume ratio of 2:1, it had an enhanced toxicity against black-chested termites. 12 CFU / mLSM1 and 2×10 8 When Conidia / mL Bb was mixed at a volume ratio of 1:1, the actual toxicity index of the mixture was 195.78, the theoretical toxicity index was 100.44, and the co-toxicity coefficient was 194.92, which was much greater than 120. Therefore, 2.63×10 12 CFU / mL SM1 and 2×10 8 When Conidia / mL Bb was mixed in a volume ratio of 1:1, it had an enhanced toxicity against black-chested termites. 12 CFU / mLSM1 and 2×10 8 When Conidia / mL Bb was mixed at a volume ratio of 1:2, the actual toxicity index of the mixture was 231.98, the theoretical toxicity index was 100.59, and the co-toxicity coefficient was 230.62, which was much greater than 120. Therefore, 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Bb has enhanced toxicity against black-chested termites when mixed in a volume ratio of 1:2.

[0121] Table 16 Co-toxicity coefficient of SM1 and Bb on black-chested Reticulitermes

[0122]

[0123] 9 Toxicity test of the combination of Serratia marcescens SM1 and Metarhizium anisopliae (Ma) against Reticulitermes nigrofens

[0124] From Table 17, we can see that 2.63×10 12 CFU / mL SM1 against black-chested termites LT 50 16.71h; 2×10 8 LT of Conidia / mL Ma against Reticulitermes fuscipiens 50 38.02h. 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Ma was mixed at a volume ratio of 2:1 to LT of black-chested termites. 50 9.33h; 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Ma was mixed in a volume ratio of 1:1 to LT of black-chested termites. 50 8.17h; 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Ma was mixed at a volume ratio of 1:2 to LT of black-chested termites. 50 It is 7.67h.

[0125] Table 17 Toxicity determination of SM1 and Ma to black-chested termites

[0126]

[0127] According to Table 18, taking SM1 as the standard agent, the toxicity index of Ma is 43.94. 12 CFU / mL SM1 and 2×10 8 When Conidia / mL Ma was mixed at a volume ratio of 2:1, the actual toxicity index of the mixture was 179.08, the theoretical toxicity index was 81.31, and the co-toxicity coefficient was 220.23, which was much greater than 120. Therefore, 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Ma has enhanced toxicity to black-chested termites when mixed in a volume ratio of 2:1. 12 CFU / mLSM1 and 2×10 8When Conidia / mL Ma was mixed at a volume ratio of 1:1, the actual toxicity index of the mixture was 204.41, the theoretical toxicity index was 71.97, and the co-toxicity coefficient was 284.02, which was much greater than 120. Therefore, 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Ma has enhanced toxicity to black-chested termites when mixed in a volume ratio of 1:1. 12 CFU / mLSM1 and 2×10 8 When Conidia / mL Ma was mixed at a volume ratio of 1:2, the actual toxicity index of the mixture was 217.72, the theoretical toxicity index was 62.63, and the co-toxicity coefficient was 347.64, which was much greater than 120. Therefore, 2.63×10 12 CFU / mL SM1 and 2×10 8 Conidia / mL Ma has enhanced toxicity against black-chested termites when mixed in a volume ratio of 1:2.

[0128] Table 18 Co-toxicity coefficient of SM1 and Ma complex to black-chested Reticulitermes

[0129]

[0130] 10 Toxicity test of the combination of Serratia marcescens SM1 and ivermectin against Reticulitermes nigropunctatus

[0131] From Table 19, we can see that 8.75×10 10 CFU / mL SM1 against black-chested termites LT 50 The concentration of ivermectin was 5.71×10 -5 mol / L 50 61.44h. 8.75×10 10 CFU / mL SM1 and 5.71×10 -5 When 100 mol / L ivermectin was mixed at a volume ratio of 2:1, the LT 50 20.40h; 8.75×10 10 CFU / mL SM1 and 5.71×10 -5 When 1:1 mol / L ivermectin is mixed in a volume ratio of 1:1, the LT 50 19.18h; 8.75×10 10 CFU / mL SM1 and 5.71×10 -5 When 1:2 mol / L ivermectin was mixed in a volume ratio of 1:2, the LT 50 It is 23.63h.

[0132] Table 19 Toxicity determination of Serratia marcescens (SM1) and ivermectin against Reticulitermes nigromaculata

[0133]

[0134] As shown in Table 20, with ivermectin as the standard agent, the toxicity index of SM1 is 29.80. 10 CFU / mL SM1 and 5.71×10 -5 When 1 mol / L ivermectin was compounded at a volume ratio of 2:1, the actual toxicity index of the mixture was 301.18, the theoretical toxicity index was 53.20, and the calculated toxicity co-toxicity coefficient was 566.12, which was much larger than 120, indicating that 8.75×10 10 CFU / mLSM1 and 5.71×10 -5 mol / L ivermectin in a volume ratio of 2:1 has a synergistic effect on the toxicity of black-chested termites. 10 CFU / mL SM1 and 5.71×10 -5 When 1 mol / L ivermectin was compounded at a volume ratio of 1:1, the actual toxicity index of the mixture was 320.30, the theoretical toxicity index was 64.90, and the calculated toxicity co-toxicity coefficient was 493.53, which was much larger than 120, indicating that 8.75×10 10 CFU / mL SM1 and 5.71×10 -5 mol / L ivermectin in a volume ratio of 1:1 has a synergistic effect on the toxicity of black-chested termites. 10 CFU / mL SM1 and 5.71×10 -5 When 1 mol / L ivermectin was compounded at a volume ratio of 1:2, the actual toxicity index of the mixture was 259.98, the theoretical toxicity index was 76.60, and the calculated toxicity co-toxicity coefficient was 339.40, which was much larger than 120, indicating that 8.75×10 10 CFU / mL SM1 and 5.71×10 -5 The 1:2 volume ratio of mol / L ivermectin had a synergistic effect on the toxicity of R. nigrotermes.

[0135] Table 20 Co-toxicity coefficient of Serratia marcescens (SM1) and ivermectin to black-chested termites

[0136]

Claims

1. A compound agent for controlling termites based on Serratia marcescens, characterized in that: The compound drug composition is Serratia marcescens, and an insect growth regulator or pathogenic fungus or a biogenic pesticide or a chemical agent; The insect growth regulator is lufenuron or buprofezin or hydrazine; The pathogenic fungus is Beauveria bassiana or Metarhizium anisopliae; The biogenic pesticide is ivermectin; The chemical agent is emamectin benzoate or indoxacarb; The bacterial count of Serratia marcescens was 7.75×10 8 ~2.63×10 12 CFU / mL; The pathogenic fungus was 2×10 8 Conidia / mL; The biogenic pesticide is 0.01 to 0.05 mg / mL; The concentration of the insect growth regulator is 1 to 5.11 mg / mL; The concentration of the chemical agent is 0.01-0.02 mg / mL.

2. The compound medicine according to claim 1, characterized in that: When the compound pharmaceutical composition is a compound of Serratia marcescens and thiamethoxam, the Serratia marcescens 7.75×10 8 ~8.75×10 10 CFU / mL, the concentration of thiamethoxam is 1 to 3.05 mg / mL, and the compound volume ratio is 9:1 to 1:

2.

3. The compound medicine according to claim 1, characterized in that: When the compound pharmaceutical composition is Serratia marcescens and pathogenic fungi, the Serratia marcescens is 2.63×10 12 CFU / mL, pathogenic fungi are 2×10 8 Conidia / mL; compound volume ratio 2:1-1:

2.

4. The compound medicine according to claim 1, characterized in that: When the compound pharmaceutical composition is Serratia marcescens and hydrazine, the Serratia marcescens is 7.75×10 8 CFU / mL, the food inhibitor is 1 mg / ml, and the compound volume ratio is 5:1-1:

9.

5. The compound medicine according to claim 1, characterized in that: When the compound pharmaceutical composition is Serratia marcescens and lufenuron, the Serratia marcescens is 8.75×10 10 CFU / mL, the concentration of lufenuron is 5.11 mg / mL, and the compound volume ratio is 2:1 to 1:

2.

6. The compound medicine according to claim 1, characterized in that: When the compound pharmaceutical composition is Serratia marcescens and emamectin benzoate or indoxacarb, the Serratia marcescens is 7.75×10 8 CFU / mL, the concentration of emamectin benzoate is 0.02 mg / mL, the concentration of indoxacarb is 0.01 mg / mL, and the volume ratio of the Serratia marcescens and emamectin benzoate or indoxacarb is 5:1-1:

9.

7. The compound medicine according to claim 1, characterized in that: The compound medicine is 7.75×10 8 When CFU / mL of Serratia marcescens is mixed with 0.01 mg / mL of ivermectin, the volume ratio is 1:5-1:

9.

8. The compound medicine according to claim 1, characterized in that: The compound medicine 7.75×10 8 ~8.75×10 10 When CFU / mL of Serratia marcescens is mixed with 0.01-0.05 mg / mL of ivermectin, the mixed volume ratio is 5:1-1:

9.

9. The compound medicine according to claim 2, characterized in that: The composite volume ratio of Serratia marcescens to thiamethoxam is 1:

1.

10. The compound medicine according to any one of claims 1 to 9, characterized in that: The termites are black-winged termites or black-chested termites.

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