Artificial feed for cantheconidea furcellata, preparation method of artificial feed and method for feeding cantheconidea furcellata

Abstract

The invention provides an artificial feed for cantheconidea furcellata, a preparation method of the artificial feed and a method for feeding the cantheconidea furcellata, and relates to the technical field of breeding of the cantheconidea furcellata. The artificial feed for cantheconidea furcellata comprises fresh larvae of spodoptera frugiperda, fresh egg liquid, pure milk, ethyl p-hydroxybenzoate, ascorbic acid, vitamin B complex, royal jelly and choline chloride. According to the method, low-cost manual rapid large-scale breeding of the cantheconidea furcellata is achieved, the survival rate of nymphs is high, the breeding cost is low, the egg laying amount of adults is high, large-scale breeding of the cantheconidea furcellata is facilitated, and the problems that in the large-scale breeding process of the cantheconidea furcellata, the breeding rate is low, and the cost is high due to the fact that the cantheconidea furcellata severely depends on natural hosts for breeding are successfully solved; and a new way is opened for controlling the lepidoptera pests.

Description

Technical Field

[0001] The present application relates to the technical field of breeding of Psoralea corytatus, and in particular to an artificial feed for Psoralea corytatus and a preparation method thereof, and a method for raising Psoralea corytatus. Background Art

[0002] Pseudocerma sutchuenensis Eocanthecona furcellata Wolff belongs to the order Hemiptera, the family Pentatomidae, the subfamily Pentatominae, and the genus Sphenodon. It is an important predatory natural enemy insect, and has good application prospects in the prevention and control of agricultural and forestry pests such as the Spodoptera litura, the fall armyworm, the armyworm, and the yellow borer. This predatory stink bug is an important biological control resource in my country, with broad application prospects and will generate significant economic benefits and ecological value. Artificial mass reproduction and release in the field to control pests has become an important way for the application of Sphenodon to biological control of pests.

[0003] At present, there is a serious lack of large-scale breeding technology for the fork-horned bug, the cost of raising natural hosts is high, and research on artificial feed is insufficient, which seriously affects the widespread application of the fork-horned bug in agricultural and forestry production. Therefore, it is very important to develop economical and effective artificial feed to breed the fork-horned bug on a large scale and promote its widespread application in the field. Summary of the invention

[0004] In view of this, the purpose of this application is to provide an artificial feed for forked horn bugs and a preparation method thereof and a method for raising forked horn bugs, so as to solve the problems of low nymph survival rate, low egg laying amount and high breeding cost in feeding forked horn bugs with artificial feed in the prior art. The technical solution adopted by this application to solve the above technical problems is: An embodiment of the present invention provides an artificial feed for Spodoptera exigua, which comprises fresh fall armyworm larvae, fresh egg liquid, pure milk, ethyl paraben, ascorbic acid, vitamin B complex, royal jelly and choline chloride.

[0005] In some embodiments, the artificial feed for the forked horn bug includes, by weight: 18 to 21 parts of fresh fall armyworm larvae, 38 to 42 parts of fresh egg liquid, 38 to 42 parts of pure milk, 0.38 to 0.42 parts of ethyl paraben, 0.38 to 0.42 parts of ascorbic acid, 0.038 to 0.042 parts of complex vitamin B, 0.0018 to 0.0021 parts of royal jelly, and 0.075 to 0.082 parts of choline chloride.

[0006] Accordingly, an embodiment of the present invention provides a method for preparing an artificial feed for Pseudocerma sutchus, comprising the following steps: crushing fresh fall armyworm larvae and vitamin B complex to obtain a first slurry; Mixing fresh egg liquid, pure milk, ethyl paraben, ascorbic acid, royal jelly and choline chloride to obtain a second slurry; The first slurry and the second slurry are evenly mixed to obtain artificial feed for Pseudocerma sutchuenensis.

[0007] In some embodiments, aluminum foil is cut into a diameter of D1, placed in the mold hole and close to the bottom of the hole, the prepared liquid artificial feed for forked horn bugs is sucked into the hole with a disposable dropper, and then the hole is sealed with plastic wrap and then covered with a lid, and placed in a freezer with a temperature below T1 for frozen storage.

[0008] In some embodiments, D1 is 18 mm to 22 mm; and / or m is 0.4g to 0.6g; and / or T1 is -12℃~-8℃.

[0009] Accordingly, an embodiment of the present invention provides a method for raising a forked horn bug using the artificial feed for a forked horn bug or the artificial feed for a forked horn bug prepared by the method, comprising the following steps: Provide egg masses of the forked-horned bug, and then hatch to obtain nymphs; Using the artificial feed for pronghorn bugs or the artificial feed for pronghorn bugs prepared by the method to feed nymphs to obtain adults; The artificial feed for pronghorn bugs or the artificial feed for pronghorn bugs prepared by the method is used to feed adult bugs, and egg masses are collected.

[0010] In some embodiments, a cylindrical box is provided, a paper towel is placed at the bottom of the cylindrical box and sprayed with water, and the egg masses of the forked-horned bug are placed in the cylindrical box, and N egg masses of the forked-horned bug are placed in each cylindrical box, the diameter of the cylindrical box is D2, the height is X1, and the box is placed in an incubator at a temperature of T2, a humidity of RH1, and a light condition of L1:D'1. Water is sprayed into the cylindrical box every day to keep it moist until the egg masses of the forked-horned bug hatch into nymphs; and / or The rearing conditions are T4 temperature, RH3 humidity, and light conditions L3:D'3. The adult stocking density is ρ2, and 2 folded A4 paper blocks are added. The artificial diet of the forked-horned stink bug is fed every day, and the feeding amount is M5. When the adults start to lay eggs, the egg masses on the folded paper blocks are cut off every day, and the folded paper after the egg masses are cut off is continued to be placed in the food-grade plastic bottle to continue laying eggs. When 1 / 3 of the folded paper block remains, new folded paper is replaced, and egg masses are collected every day. The residue in the food-grade plastic bottle for insect breeding is cleaned up to keep the food-grade plastic bottle clean, and the cut egg masses are placed in an incubator for incubation or stored in a refrigerator at 8°C.

[0011] In some embodiments, N is 15 to 17 blocks; and / or D2 is 9cm~11cm; and / or X1 is 4.5cm~5.5cm; and / or T2 is 26℃~28℃; and / or RH1 is 65% to 75%; and / or L1:D'1 is 16:8; and / or T4 is 26℃~28℃; and / or RH3 is 65%~75%; and / or L3: D'3 is 16:8; and / or ρ2 is 40 heads / bottle to 50 heads / bottle; and / or M5 is 35 mg~45 mg / head.

[0012] In some embodiments, a cylindrical box is provided again, a paper towel is placed on the bottom of the cylindrical box, and the nymphs of the forked horn bugs are added to the cylindrical box at a density of ρ1 for feeding. The feeding conditions are cultured in an incubator at a temperature of T3, a humidity of RH2, and a light condition of L2:D'2. The nymphs of the forked horn bugs are fed with honey water for the first 2 days, and the nymphs of the forked horn bugs are fed with artificial feed while being fed with honey water on the 3rd day. On the 4th day, the nymphs of the forked horn bugs are transferred to a food-grade plastic bottle with a volume of V for feeding, and a folded A4 paper block is added, and then the artificial feed of the forked horn bugs is added once a day; the diameter of the food-grade plastic bottle is D3, and the height is X2; the amount of artificial feed of the forked horn bugs added to the 2nd instar nymphs is M1, the amount of artificial feed of the forked horn bugs added to the 3rd instar nymphs is M2, the amount of artificial feed of the forked horn bugs added to the 4th instar nymphs is M3, and the amount of artificial feed of the forked horn bugs added to the 5th instar nymphs is M4; The method of feeding honey water includes sticking a cotton block containing honey water with a mass concentration of 8% to 12% on the inner box wall of a cylindrical box; The method for adding artificial feed to the forked-horned bug includes cutting the nanosponge into 40mm×40mm squares as a food table, digging a circular hole with a diameter of 20mm in the middle, then providing a 2cm aluminum foil and folding it into a disc shape and placing it in the circular hole, and then adding the prepared artificial feed to the forked-horned bug on the aluminum foil in the circular hole.

[0013] In some embodiments, ρ1 is 90 heads / box to 110 heads / box; and / or T3 is 26℃~28℃; and / or RH2 is 65%~75%; and / or L2: D'2 is 16:8; and / or V is 1L~1.5L; and / or D3 is 10cm~12cm; and / or X2 is 14cm~16cm; and / or M1: 100 heads / 90 mg~110 mg; and / or M2: 100 heads / 190 mg~210 mg; and / or M3: 100 heads / 700 mg~900 mg; and / or M4 is 100 heads / 1500 mg~1700 mg.

[0014] In the present application, fresh fall armyworm larvae are used, which contain hemolymph, and meet the needs of the forked horn bug for insect-derived protein and fat substances, and also stimulate the feeding of the forked horn bug; fresh egg liquid provides protein, cholesterol, etc. for the forked horn bug; pure milk provides protein for the forked horn bug; ascorbic acid is an essential nutrient for the growth and development of the forked horn bug, and is also a feeding stimulant; complex vitamin B meets the needs of the forked horn bug for B vitamins; royal jelly meets the needs of the forked horn bug for amino acids, B vitamins and steroid substances; choline chloride meets the needs of the forked horn bug for choline substances; ethyl paraben is used for artificial feed preservation and extended storage period. The artificial feed of the forked horn bug of the present invention is a liquid feed, which meets the requirements of the forked horn bug for nutritional growth, and also adapts to the feeding habits of the forked horn bug through the piercing-sucking mouthparts, piercing the mouthparts into the prey and directly sucking liquid.

[0015] The invention can breed the forked horn bug on a large scale, has low artificial feed cost, has a wide source of feed raw materials, is easy to prepare, and has a simple breeding process, and can be prepared and used at any time, thereby overcoming the disadvantage of a shortage of food insect sources when a large number of forked horn bugs are needed to breed, greatly improving the breeding efficiency, and improving the survival rate of the forked horn bugs. The survival rate, larval stage, pupation rate, and emergence rate of the forked horn bugs are comparable to the breeding results of fresh fall armyworm larvae.

[0016] The fall armyworm larvae of the present invention are self-raised by the inventor, providing the needs of the forked horn bug for protein and fat, and stimulating the forked horn bug to feed. The present invention is groundbreaking in adding fall armyworm larvae to the feed of the forked horn bug, because through long-term research, fall armyworm larvae are the best food insect source for the forked horn bug, which can stimulate the forked horn bug to feed, improve the survival rate of the larvae, promote the normal metabolism of the forked horn bug, and avoid malnutrition. Finally, the research obtained a method for large-scale breeding of forked horn bug groups with low breeding cost and high nymph survival rate.

[0017] The present invention realizes low-cost artificial rapid large-scale breeding of fork-horned bugs, has a high nymph survival rate, low breeding cost, and high adult egg-laying amount, which is conducive to large-scale breeding of fork-horned bugs and successfully solves the problems of low reproduction rate and high cost in the large-scale breeding process of fork-horned bugs that heavily rely on natural hosts for breeding, and will open up a new way for the control of lepidopteran pests. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings of the embodiments are briefly introduced below. Obviously, the drawings described below only relate to some embodiments of the present application, but are not intended to limit the present application, wherein: Figure 1 Figure 2 is an artificial feed for Pseudocerma sutchuenensis prepared by the present invention; Figure 2 This is a diagram of the fifth instar nymph of the pronghorn bug of the present invention; Figure 3 It is a picture of the adult of the forked-horned bug of the present invention. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work belong to the scope of protection of the present application. In addition, it should be understood that the specific implementation methods described herein are only used to illustrate and explain the present application, and are not used to limit the present application.

[0020] In this application, unless otherwise stated, directional words such as "upper" and "lower" generally refer to the upper and lower parts of the device in actual use or working state, specifically the drawing direction in the accompanying drawings; while "inner" and "outer" refer to the outline of the device. In addition, in the description of this application, the term "including" means "including but not limited to". The terms first, second, third, etc. are used only as labels and do not impose numerical requirements or establish an order.

[0021] In this application, "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0022] In this application, "at least one" means one or more, and "plurality" means two or more. "One or several", "at least one of the following" or similar expressions refer to any combination of these items, including any combination of single items or plural items. For example, "at least one of a, b, or c", or "at least one of a, b, and c" can all mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple.

[0023] Various embodiments of the present application may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present application; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0024] In a first aspect, an embodiment of the present invention provides an artificial feed for Spodoptera exigua, which comprises fresh fall armyworm larvae, fresh egg liquid, pure milk, ethyl paraben, ascorbic acid, vitamin B complex, royal jelly and choline chloride.

[0025] In the present application, fresh fall armyworm larvae are used, which contain hemolymph, and meet the needs of the forked horn bug for insect-derived protein and fat substances, and also stimulate the feeding of the forked horn bug; fresh egg liquid provides protein, cholesterol, etc. for the forked horn bug; pure milk provides protein for the forked horn bug; ascorbic acid is an essential nutrient for the growth and development of the forked horn bug, and is also a feeding stimulant; complex vitamin B meets the needs of the forked horn bug for B vitamins; royal jelly meets the needs of the forked horn bug for amino acids, B vitamins and steroid substances; choline chloride meets the needs of the forked horn bug for choline substances; ethyl paraben is used for artificial feed preservation and extended storage period. The artificial feed of the forked horn bug of the present invention is a liquid feed, which meets the requirements of the forked horn bug for nutritional growth, and also adapts to the feeding habits of the forked horn bug through the piercing-sucking mouthparts, piercing the mouthparts into the prey and directly sucking liquid.

[0026] The invention can breed the forked horn bug on a large scale, has low artificial feed cost, has a wide source of feed raw materials, is easy to prepare, and has a simple breeding process, and can be prepared and used at any time, thereby overcoming the disadvantage of a shortage of food insect sources when a large number of forked horn bugs are needed to breed, greatly improving the breeding efficiency, and improving the survival rate of the forked horn bugs. The survival rate, larval stage, pupation rate, and emergence rate of the forked horn bugs are comparable to the breeding results of fresh fall armyworm larvae.

[0027] The fall armyworm larvae of the present invention are self-raised by the inventor, providing the needs of the forked horn bug for protein and fat, and stimulating the forked horn bug to feed. The present invention is groundbreaking in adding fall armyworm larvae to the feed of the forked horn bug, because through long-term research, fall armyworm larvae are the best food insect source for the forked horn bug, which can stimulate the forked horn bug to feed, improve the survival rate of the larvae, promote the normal metabolism of the forked horn bug, and avoid malnutrition. Finally, the research obtained a method for large-scale breeding of forked horn bug groups with low breeding cost and high nymph survival rate.

[0028] The present invention realizes low-cost artificial rapid large-scale breeding of fork-horned bugs, has a high nymph survival rate, low breeding cost, and high adult egg-laying amount, which is conducive to large-scale breeding of fork-horned bugs and successfully solves the problems of low reproduction rate and high cost in the large-scale breeding process of fork-horned bugs that heavily rely on natural hosts for breeding, and will open up a new way for the control of lepidopteran pests.

[0029] In some embodiments, the artificial feed for the forked-horned bug includes, by weight: 18 to 21 parts of fresh fall armyworm larvae, 38 to 42 parts of fresh egg liquid, 38 to 42 parts of pure milk, 0.38 to 0.42 parts of ethyl paraben, 0.38 to 0.42 parts of ascorbic acid, 0.038 to 0.042 parts of vitamin B complex, 0.0018 to 0.0021 parts of royal jelly, and 0.075 to 0.082 parts of choline chloride.

[0030] Furthermore, the artificial feed for the forked horn bug comprises, by weight: 18 to 21 parts of fresh fall armyworm larvae, preferably 18.5 to 20.5 parts of fresh fall armyworm larvae, more preferably 19 to 20 parts of fresh fall armyworm larvae, more preferably 19.5 parts of fresh fall armyworm larvae; 38 to 42 parts of fresh egg liquid, preferably 38.5 to 41.5 parts of fresh egg liquid, more preferably 39 to 41 parts of fresh egg liquid, more preferably 40 parts of fresh egg liquid; pure beef 38 to 42 parts of milk, preferably 38.5 to 41.5 parts of pure milk, more preferably 39 to 41 parts of pure milk, more preferably 40 parts of pure milk; 0.38 to 0.42 parts of ethyl p-hydroxybenzoate, preferably 0.39 to 0.41 parts of ethyl p-hydroxybenzoate, more preferably 0.4 parts of ethyl p-hydroxybenzoate; 0.38 to 0.42 parts of ascorbic acid, preferably 0.39 to 0.41 parts of ascorbic acid, more preferably 0.4 parts of ascorbic acid; vitamin B complex 0.038 to 0.042 parts, preferably 0.039 to 0.041 parts of vitamin B complex, more preferably 0.040 parts of vitamin B complex; 0.0018 to 0.0021 parts of royal jelly, preferably 0.0019 to 0.0020 parts of royal jelly, more preferably 0.00195 parts of royal jelly; 0.075 to 0.082 parts of choline chloride, preferably 0.078 to 0.080 parts of choline chloride, more preferably 0.079 parts of choline chloride.

[0031] In a second aspect, an embodiment of the present invention provides a method for preparing an artificial feed for a forked horned bug, comprising the following steps: S01, crushing fresh fall armyworm larvae and vitamin B complex to obtain a first slurry; S02, mixing fresh egg liquid, pure milk, ethyl paraben, ascorbic acid, royal jelly and choline chloride to obtain a second slurry; S03, mixing the first slurry and the second slurry evenly to obtain artificial feed for Pseudocerma spp.

[0032] In the S01: In some embodiments, fresh fall armyworm larvae and vitamin B complex are crushed into a first slurry using a Joyoung food processor.

[0033] In said S03: In some embodiments, aluminum foil is cut into a diameter of D1, placed in the mold hole and close to the bottom of the hole, the prepared liquid artificial feed for forked horn bugs is sucked into the hole with a disposable dropper, and then the hole is sealed with plastic wrap and then covered with a lid, and placed in a freezer with a temperature below T1 for frozen storage.

[0034] In some embodiments, D1 is 18 mm to 22 mm, for example, it can be 18 mm, 19 mm, 20 mm, 21 mm, 22 mm, etc.

[0035] m is 0.4 g to 0.6 g, for example, it can be 0.4 g, 0.45 g, 0.5 g, 0.55 g, 0.6 g, etc.

[0036] T1 is -12°C to -8°C, for example, it can be -12°C, -11°C, -10°C, -9°C, -8°C, etc.

[0037] In some embodiments, the disposable dropper is a disposable plastic dropper with a rubber tip.

[0038] In a third aspect, an embodiment of the present invention provides a method for raising a forked horn bug using the artificial feed for a forked horn bug or the artificial feed for a forked horn bug prepared by the method, comprising the following steps: S11, providing egg masses of the forked-horned bug, which then hatch to obtain nymphs; S12, raising nymphs with the artificial feed for Psoralea serrata or the artificial feed for Psoralea serrata prepared by the method to obtain adults; S13, raising adult bugs with the artificial diet for pronghorn bugs or the artificial diet for pronghorn bugs prepared by the method, and collecting egg masses.

[0039] In the S11: In some embodiments, eggs of the pronghorn bug are introduced from Guangdong Zhixin Biological Co., Ltd., and the pronghorn bug egg masses are cultured and collected.

[0040] In some embodiments, a cylindrical box is provided, a paper towel is placed at the bottom of the cylindrical box and sprayed with water, and egg masses of the forked-horned bug are placed in the cylindrical box. N egg masses of the forked-horned bug are placed in each cylindrical box. The cylindrical box has a diameter of D2 and a height of X1. The box is placed in an incubator at a temperature of T2, a humidity of RH1, and a light condition of L1:D'1. Water is sprayed into the cylindrical box every day to keep it moist until nymphs are hatched from the egg masses of the forked-horned bug.

[0041] Furthermore, N is 15 blocks to 17 blocks, for example, it can be 15 blocks, 16 blocks, 17 blocks, etc.

[0042] D2 is 9 cm to 11 cm, for example, it can be 9 cm, 10 cm, 11 cm, etc.

[0043] X1 is 4.5cm~5.5cm, for example, it can be 4.5cm, 4.8cm, 5cm, 5.2cm, 5.4cm, 5.5cm, etc.

[0044] T2 is 26°C to 28°C, for example, it can be 26°C, 27°C, 28°C, etc.

[0045] RH1 is 65% to 75%, for example, it can be 65%, 68%, 70%, 72%, 74%, 75%, etc.

[0046] L1:D'1 is 16:8.

[0047] In S12: In some embodiments, a cylindrical box is provided again, which needs to be breathable, the top of the cylindrical box is removed and non-woven fabric is pasted, and a paper towel is padded on the bottom of the cylindrical box. The nymphs of the forked-horned bugs are added to the cylindrical box at a density of ρ1 for breeding. The breeding conditions are cultured in an incubator at a temperature of T3, a humidity of RH2, and a light condition of L2:D'2. Honey water is fed for the first 2 days, and the 2nd-instar nymphs of the forked-horned bugs are fed with artificial feed while feeding the honey water on the 3rd day. On the 4th day, the nymphs of the forked-horned bugs are transferred to a food-grade plastic bottle with a volume of V for breeding, and a folded A4 paper block is added, and then the artificial feed of the forked-horned bugs is added once a day; the diameter of the food-grade plastic bottle is D3, and the height is X2; the amount of artificial feed added to the 2nd-instar nymphs is M1, the amount of artificial feed added to the 3rd-instar nymphs is M2, the amount of artificial feed added to the 4th-instar nymphs is M3, and the amount of artificial feed added to the 5th-instar nymphs (such as Figure 2 The additive amount of the artificial diet for the forked-horned bug (as shown) is M4.

[0048] It can be understood that the 3rd instar nymphs, the 4th instar nymphs and the 5th instar nymphs only need to be fed with the artificial diet for the forked horn bug of the present invention, and do not need to be fed with honey water.

[0049] It can also be understood that the 5th instar nymphs emerge as adults. Figure 3 shown.

[0050] In some embodiments, a ventilation hole is opened on the top of the cylindrical box.

[0051] In some embodiments, the bottom of the food-grade plastic bottle is removed, and non-woven fabric is pasted on the bottom for the purpose of ventilation.

[0052] Furthermore, ρ1 is 90 heads / box to 110 heads / box, for example, it can be 90 heads / box, 100 heads / box, 110 heads / box, etc.

[0053] T3 is 26°C to 28°C, for example, it can be 26°C, 27°C, 28°C, etc.

[0054] RH2 is 65% to 75%, for example, it can be 65%, 68%, 70%, 72%, 74%, 75%, etc.

[0055] L2:D'2 is 16:8.

[0056] V is 1L~1.5L, for example, it can be 1L, 1.2L, 1.3L, 1.4L, 1.5L, etc.

[0057] D3 is 10cm~12cm, for example, it can be 10cm, 11cm, 12cm, etc.

[0058] X2 is 14cm~16cm, for example, it can be 14cm, 15cm, 16cm, etc.

[0059] M1 is 100 heads / 90 mg to 110 mg, for example, it can be 100 heads / 90 mg, 100 heads / 100 mg, 100 heads / 110 mg, etc.

[0060] M2 is 100 heads / 190 mg to 210 mg, for example, it can be 100 heads / 190 mg, 100 heads / 200 mg, 100 heads / 210 mg, etc.

[0061] M3 is 100 heads / 700 mg~900 mg, for example, it can be 100 heads / 700 mg, 100 heads / 800 mg, 100 heads / 900 mg, etc.

[0062] M4 is 100 heads / 1500 mg~1700 mg, for example, it can be 100 heads / 1500 mg, 100 heads / 1600 mg, 100 heads / 1700 mg, etc.

[0063] Further, the method of feeding honey water includes sticking a cotton block containing honey water with a mass concentration of 8% to 12% on the inner box wall of the cylindrical box. It can be understood that this makes it convenient for the nymphs of the forked horn bug to feed.

[0064] Furthermore, the diameter of the cotton block is 1.2 cm to 1.8 cm, for example, it can be 1.2 cm, 1.3 cm, 1.4 cm, 1.5 cm, 1.6 cm, 1.7 cm, 1.8 cm, etc.

[0065] The thickness of the cotton block is 0.1 cm to 0.3 cm, for example, it can be 0.1 cm, 0.12 cm, 0.15 cm, 0.16 cm, 0.18 cm, 0.2 cm, 0.22 cm, 0.25 cm, 0.28 cm, 0.3 cm, etc.

[0066] It can be understood that the number of cotton pieces is 1.

[0067] Furthermore, the method for adding artificial feed for the forked-horned bug includes cutting the nanosponge into 40mm×40mm squares as a food table, digging a circular hole with a diameter of 20mm in the middle, then providing a 2cm aluminum foil and folding it into a disc shape and placing it in the circular hole, and then adding the prepared artificial feed for the forked-horned bug to the aluminum foil in the circular hole.

[0068] In S13: In some embodiments, the rearing conditions are T4 temperature, RH3 humidity, and light conditions of L3:D'3. The adult stocking density is ρ2, and 2 folded A4 paper blocks are added. The artificial feed for the forked-horned bug is fed every day, and the feeding amount is M5. When the adults start to lay eggs, the egg masses on the folded paper blocks are cut off every day, and the folded paper after the egg masses are cut off is continued to be placed in a food-grade plastic bottle to continue laying eggs. When 1 / 3 of the folded paper block remains, new folded paper is replaced, egg masses are collected every day, the residue in the food-grade plastic bottle for raising insects is cleaned, the food-grade plastic bottle is kept clean, and the cut egg masses are placed in an incubator for incubation or stored in a refrigerator at 8°C.

[0069] Furthermore, T4 is 26°C to 28°C, for example, it can be 26°C, 27°C, 28°C, etc.

[0070] RH3 is 65% to 75%, for example, it can be 65%, 68%, 70%, 72%, 74%, 75%, etc.

[0071] L3:D'3 is 16:8.

[0072] ρ2 is 40 heads / bottle to 50 heads / bottle, for example, it can be 40 heads / bottle, 42 heads / bottle, 45 heads / bottle, 48 heads / bottle, 49 heads / bottle, 50 heads / bottle, etc.

[0073] M5 is 35 mg to 45 mg / head, for example, 35 mg / head, 36 mg / head, 38 mg / head, 40 mg / head, 42 mg / head, 45 mg / head, etc.

[0074] Example 1 See also Figure 1 The artificial feed for the forked-horned bug includes the following components by weight: 19.5g fresh fall armyworm larvae, 40g fresh egg liquid, 40g pure milk, 0.4g ethyl paraben, 0.4g ascorbic acid, 0.04g vitamin B complex, 0.0019g royal jelly, and 0.08g choline chloride.

[0075] The method for preparing the artificial feed for the pronghorn bug comprises the following steps: Step 1, crush the above-mentioned weight of fresh fall armyworm larvae and vitamin B complex into a first slurry using a Joyoung food processor; Step 2, mixing the above-mentioned egg liquid, pure milk, ethyl paraben, ascorbic acid, royal jelly and choline chloride in weight to obtain a second slurry; Step 3, mixing the first slurry and the second slurry evenly to obtain artificial feed for Pseudocerma sutchuenensis; Step 4, storage: cut the aluminum foil into 20mm diameter, place it tightly in the well of the mold (24-well bacterial culture plate), use a 0.5ml disposable plastic dropper with a rubber tip to draw 0.5g of the prepared liquid artificial feed for forked horn bugs and add it into the well, 0.5g of feed in each well. After all the feed is injected, cover it with plastic wrap and then cover it with a lid, and immediately put it into a freezer below -10℃ to keep it for later use.

[0076] The method for raising the forked horn bug with the artificial feed for the forked horn bug prepared in this embodiment comprises the following steps: The eggs of the forked-horned bug introduced from Guangdong Zhixin Biological Co., Ltd. were used as the insect source after being bred indoors for several generations.

[0077] Step a, hatching management of eggs of forked horn bugs: collect the forked horn bug egg masses, put them into a round box with a diameter of 10 cm and a height of 5 cm, 16 eggs per box, spray water on the bottom with a paper towel, place them in an incubator at 27°C, a humidity of 70%, and a light condition of L:D=16:8, spray water every day to keep them moist until the egg masses hatch nymphs.

[0078] Step b, feeding and management of nymphs of forked horn bugs: Under the condition of temperature of 27°C, humidity of 70%, and light condition of L:D=16:8, select 90 2nd instar nymphs of forked horn bugs of the same size, set up 3 replicates, 30 heads / bottle. (1) The nymphs of forked horn bugs are raised at a density of 100 heads / box, and are raised in a food-grade plastic round box with a diameter of 100 mm and a height of 48 mm. The bottom of the box is padded with toilet paper, and 10% honey water cotton blocks are added every day. The cotton blocks are attached to the wall of the box for the nymphs of forked horn bugs to feed. (2) The newly hatched nymphs of forked horn bugs are fed with honey water for the first 2 days. On the 3rd day, the prepared artificial feed is fed to the 2nd instar nymphs while adding honey water, 100 heads / 100 mg. The nanosponge was cut into 40mm×40mm squares, and a circular hole with a diameter of 20mm was dug in the middle. The frozen artificial feed was then added to the aluminum foil with a diameter of 2cm and then placed in the circular hole of the nanosponge. The aluminum foil was folded into a disc shape. On the 4th day, the nymphs of the forked horn bug were transferred to a food-grade plastic bottle with a volume of 1.2L for breeding, and a folded A4 paper block was added. The specification of the plastic bottle was 110mm in diameter and 150mm in height. Artificial feed was added once a day, and the ratio of forked horn bugs to artificial feed was about 100 nymphs / 100mg for the 2nd instar nymphs, 100 nymphs / 200mg for the 3rd instar nymphs, 100 nymphs / 800mg for the 4th instar nymphs, and 100 nymphs / 1600mg for the 5th instar nymphs.

[0079] Step c, feeding and management of adult forked horn bugs: Under the conditions of temperature 27°C, humidity 70%, and light conditions L: D = 16: 8, the adult stage feeding density is 45 heads / bottle to ensure that the forked horn bugs have sufficient activity space, and 2 folding paper blocks are added to facilitate the forked horn bugs to climb and lay eggs. Feed artificial feed every day, and the feeding amount is 40 mg / head. When the adults begin to lay eggs, the egg blocks on the folding paper blocks are cut off every day, and placed in a food-grade plastic bottle to continue laying eggs. When 1 / 3 of the folding paper blocks remain, replace new folding paper. Collect egg blocks every day, clean the residue in the insect food-grade plastic bottle, and keep the food-grade plastic bottle clean. After the forked horn bugs lay eggs, take out the eggs in time, put them in an incubator for incubation or store them in a refrigerator at 8°C.

[0080] Example 2 The artificial feed for the forked-horned bug includes the following components by weight: 18g of fresh fall armyworm larvae, 38g of fresh egg liquid, 38g of pure milk, 0.38g of ethyl paraben, 0.38g of ascorbic acid, 0.038g of vitamin B complex, 0.0018g of royal jelly, and 0.075g of choline chloride.

[0081] The preparation method of the artificial feed for the pronghorn bug is the same as that in Example 1.

[0082] The method for raising the forked horn bug with the artificial feed for the forked horn bug prepared in this example is the same as that in Example 1.

[0083] Example 3 The artificial feed for the forked-horned bug includes the following components by weight: 21g of fresh fall armyworm larvae, 42g of fresh egg liquid, 42g of pure milk, 0.42g of ethyl paraben, 0.42g of ascorbic acid, 0.042g of vitamin B complex, 0.0021g of royal jelly, and 0.082g of choline chloride.

[0084] The preparation method of the artificial feed for the pronghorn bug is the same as that in Example 1.

[0085] The method for raising the forked horn bug with the artificial feed for the forked horn bug prepared in this example is the same as that in Example 1.

[0086] Example 4 The artificial feed for the forked-horned bug includes the following components by weight: 19g of fresh fall armyworm larvae, 39g of fresh egg liquid, 39g of pure milk, 0.39g of ethyl paraben, 0.39g of ascorbic acid, 0.039g of vitamin B complex, 0.0019g of royal jelly, and 0.078g of choline chloride.

[0087] The preparation method of the artificial feed for the pronghorn bug is the same as that in Example 1.

[0088] The method for raising the forked horn bug with the artificial feed for the forked horn bug prepared in this example is the same as that in Example 1.

[0089] Example 5 The artificial feed for the forked-horned bug includes the following components by weight: 20g of fresh fall armyworm larvae, 40g of fresh egg liquid, 40g of pure milk, 0.41g of ethyl paraben, 0.41g of ascorbic acid, 0.041g of vitamin B complex, 0.0020g of royal jelly, and 0.080g of choline chloride.

[0090] The preparation method of the artificial feed for the pronghorn bug is the same as that in Example 1.

[0091] The method for raising the forked horn bug with the artificial feed for the forked horn bug prepared in this example is the same as that in Example 1.

[0092] According to the formulas of the above embodiments, as many artificial feeds for Psoralea serrata as possible are prepared to meet the needs of feeding Psoralea serrata.

[0093] Comparative Example 1 Select 2-instar nymphs of Spodoptera exigua of the same size, add fresh larvae of Spodoptera exigua of the same age and size as the Spodoptera exigua, and raise them in a light incubator with a temperature of 26°C-28°C, an air humidity of 70%-80%, and a photoperiod of 16h during the day and 8h at night. Record and observe the survival number, developmental period, pre-oviposition period, and egg laying amount of the Spodoptera exigua nymphs.

[0094] Comparative Example 2 Select 2-instar nymphs of the same size as the forked-horned bug and add fresh beet armyworm larvae of the same age and size as the forked-horned bug, and raise them in a light incubator with a temperature of 26°C-28°C, an air humidity of 70%-80%, and a photoperiod of 16h during the day and 8h at night. Record the survival number, developmental period, pre-oviposition period, and egg laying amount of the forked-horned bug nymphs.

[0095] Comparative Example 3 Select mealworm pupae and put them into the breeding bottles of forked horn bugs to feed the 2nd instar nymphs of forked horn bugs. Put them in the light incubator with a temperature of 26℃-28℃, air humidity of 70%-80%, and a photoperiod of 16h during the day and 8h at night. Observe and add food every day, clean the food residues in the breeding bottles, and record the survival number of forked horn bug nymphs, nymph development period, pre-oviposition period, and egg laying amount.

[0096] The effects of the method of using artificial feed for breeding Pseudocerus punctatus in Examples 1 to 5 and using larvae of fall armyworm, beet armyworm and mealworm pupae for breeding Pseudocerus punctatus in Comparative Examples 1 to 3 are shown in Table 1.

[0097] Table 1 Comparison of the effects of breeding Pseudocercus spp. in Examples 1 to 5 of the present invention and Comparative Examples 1 to 3

[0098] As can be seen from Table 1, the average survival rate of nymphs in Examples 1 to 5 of the present invention is 75.41%, the average nymph development period is 15.45 days, the average pre-oviposition period is 9.38 days, the average maximum single female single egg production is 81.73 eggs, and the average total single female egg production is 295.24 eggs. The average survival rate of nymphs in Comparative Examples 1-3 is 57.48%, the average nymph development period is 18.80 days, the average pre-oviposition period is 15.35 days, the average maximum single female egg production is 61.73 eggs, and the average total single female egg production is 210.73 eggs. In general, the nymph survival rate, the maximum single-time egg-laying amount of a single female, and the total egg-laying amount of a single female in Example 1 are only lower than those of the fresh fall armyworm larvae in Comparative Example 1, and the difference is not big, and they are significantly higher than those in Example 2, Example 3, Example 4 and Example 5; compared with Comparative Examples 2-3, the pre-oviposition time of Example 1 is shorter than that of Comparative Example 2, and significantly lower than that of Comparative Example 3; the nymph development period and the total egg-laying amount of a single female in Example 1 are similar to the results of raising fall armyworm larvae in Comparative Example 1. In general, Example 1 can achieve a similar feeding effect to Comparative Example 1, with little difference. While reducing the feeding cost, the feeding effect is the best.

[0099] The volume of 1.1775 cubic decimeters of the present invention can breed 90 to 110 nymphs, and the volume of 1.1775 cubic decimeters can breed 45 to 50 adults.

[0100] Cost accounting: In this application, fresh fall armyworm larvae: 37.8 yuan / 100g, fresh egg liquid: 1 yuan / 100g, pure milk: 0.75 yuan / 100g, ethyl paraben: 8 yuan / 100g, ascorbic acid: 3 yuan / 100g, vitamin B complex: 6 yuan / 100g, royal jelly: 25 yuan / 100g, choline chloride: 10 yuan / 100g.

[0101] 100.42 g of artificial feed for Pseudocerma sutchuenensis prepared in Example 1 of the present application cost 8.20 yuan.

[0102] The artificial feed of the forked-horned stink bug in the existing technology: maggot powder 20g (30 yuan / 100g), beef liver 15g (1.4 yuan / 100g), egg yolk 10g (1 yuan / 100g), tussah hemolymph 10ml (28 yuan / 100g), honey 8g (10 yuan / 100g), royal jelly 1g (25 yuan / 100g), trehalose 0.1g (25 yuan / 100g), yeast powder 2g (2.5 yuan / 100g), choline chloride 0.4g (3.5 yuan / 100g), ascorbic acid 0.1g ((7.5 yuan / 100g)), streptomycin sulfate 0.05g (160 yuan / 100g), fresh milk 8ml (0.75 yuan / 100g), and Chinese cabbage juice 2ml (1 yuan / 100g).

[0103] If 100 g of the artificial feed for the forked horn bug of the prior art is prepared, the cost is 13.59 yuan.

[0104] The artificial feed for the forked horn bug in the prior art weighs 76.65 g in total and costs 10.42 yuan.

[0105] From the perspective of cost, the artificial feed for forked horn bugs of the present application reduces the cost by 40% compared with the artificial feed for forked horn bugs of the prior art.

[0106] The basic concepts have been described above. Obviously, for those skilled in the art, the above detailed disclosure is only for example and does not constitute a limitation of the present application. Although not explicitly stated herein, those skilled in the art may make various modifications, improvements and amendments to the present application. Such modifications, improvements and amendments are suggested in the present application, so such modifications, improvements and amendments still belong to the spirit and scope of the exemplary embodiments of the present application.

[0107] At the same time, the present application uses specific words to describe the embodiments of the present application. For example, "one embodiment", "an embodiment", and / or "some embodiments" refer to a certain feature, structure or characteristic related to at least one embodiment of the present application. Therefore, it should be emphasized and noted that "one embodiment" or "an embodiment" or "an alternative embodiment" mentioned twice or more in different positions in this specification does not necessarily refer to the same embodiment. In addition, some features, structures or characteristics in one or more embodiments of the present application can be appropriately combined.

Claims

1. An artificial feed for Pseudocerma sutchuenensis, characterized in that: The artificial feed for the forked horn bug comprises fresh fall armyworm larvae, fresh egg liquid, pure milk, ethyl p-hydroxybenzoate, ascorbic acid, vitamin B complex, royal jelly and choline chloride.

2. The artificial feed for Pseudocerma sutchuenensis according to claim 1, characterized in that: The artificial feed for the forked-horned bug includes, by weight: 18 to 21 parts of fresh fall armyworm larvae, 38 to 42 parts of fresh egg liquid, 38 to 42 parts of pure milk, 0.38 to 0.42 parts of ethyl paraben, 0.38 to 0.42 parts of ascorbic acid, 0.038 to 0.042 parts of complex vitamin B, 0.0018 to 0.0021 parts of royal jelly, and 0.075 to 0.082 parts of choline chloride.

3. A method for preparing an artificial feed for a forked horn bug, characterized in that: The steps include: crushing fresh fall armyworm larvae and vitamin B complex to obtain a first slurry; Mixing fresh egg liquid, pure milk, ethyl paraben, ascorbic acid, royal jelly and choline chloride to obtain a second slurry; The first slurry and the second slurry are evenly mixed to obtain artificial feed for Pseudocerma sutchuenensis.

4. The method for preparing the artificial feed for Pseudocerma sutchuenensis according to claim 1, characterized in that: Cut the aluminum foil into a diameter of D1, place it in the mold hole and close to the bottom of the hole, use a disposable dropper to pipette the prepared liquid artificial feed for the forked horn bug into the hole, then seal the hole with plastic wrap and then cover it with a lid, and store it in a freezer at a temperature below T1.

5. The method for preparing the artificial feed for Pseudocerma sutchuenensis according to claim 4, characterized in that: D1 is 18mm~22mm; and / or m is 0.4g to 0.6g; and / or T1 is -12℃~-8℃.

6. A method for raising a forked horn bug using the artificial feed for a forked horn bug according to claim 1 or 2 or the artificial feed for a forked horn bug prepared by the method according to any one of claims 3 to 5, characterized in that: The steps include: Provide egg masses of the forked-horned bug, and then hatch to obtain nymphs; Using the artificial feed for pronghorn bugs or the artificial feed for pronghorn bugs prepared by the method to feed nymphs to obtain adults; The artificial feed for pronghorn bugs or the artificial feed for pronghorn bugs prepared by the method is used to feed adult bugs, and egg masses are collected.

7. The method for raising the forked horned bug according to claim 6, characterized in that: Provide a cylindrical box, pad a paper towel at the bottom of the cylindrical box and spray water, place the egg masses of the forked horn bug in the cylindrical box, put N egg masses of the forked horn bug in each cylindrical box, the diameter of the cylindrical box is D2, the height is X1, and the box is placed in an incubator at a temperature of T2, a humidity of RH1, and a light condition of L1:D'1. Spray water into the cylindrical box every day to keep it moist until the egg masses of the forked horn bug hatch into nymphs; and / or The rearing conditions are T4 temperature, RH3 humidity, and light conditions L3:D'3. The adult stocking density is ρ2, and 2 folded A4 paper blocks are added. The artificial diet of the forked-horned stink bug is fed every day, and the feeding amount is M5. When the adults start to lay eggs, the egg masses on the folded paper blocks are cut off every day, and the folded paper after the egg masses are cut off is continued to be placed in the food-grade plastic bottle to continue laying eggs. When 1 / 3 of the folded paper block remains, new folded paper is replaced, and egg masses are collected every day. The residue in the food-grade plastic bottle for insect breeding is cleaned up to keep the food-grade plastic bottle clean, and the cut egg masses are placed in an incubator for incubation or stored in a refrigerator at 8°C.

8. The method for raising the forked horned bug according to claim 7, characterized in that: N is 15-17 blocks; and / or D2 is 9cm~11cm; and / or X1 is 4.5cm~5.5cm; and / or T2 is 26℃~28℃; and / or RH1 is 65% to 75%; and / or L1:D'1 is 16:8; and / or T4 is 26℃~28℃; and / or RH3 is 65%~75%; and / or L3: D'3 is 16:8; and / or ρ2 is 40 heads / bottle to 50 heads / bottle; and / or M5 is 35 mg~45 mg / head.

9. The method for raising the forked horned bug according to claim 7, characterized in that: A cylindrical box is provided again, a paper towel is placed at the bottom of the cylindrical box, and the nymphs of the forked-horned bugs are added to the cylindrical box at a density of ρ1 for breeding. The breeding conditions are as follows: the temperature is T3, the humidity is RH2, and the light condition is L2:D'2 in an incubator. The forked-horned bugs are fed with honey water for the first 2 days, and the 2nd-instar nymphs are fed with artificial feed of the forked-horned bugs at the same time as the honey water on the 3rd day. On the 4th day, the forked-horned bugs nymphs are transferred to a food-grade plastic bottle with a volume of V for breeding, and a folded A4 paper block is added, and then the artificial feed of the forked-horned bugs is added once a day; the diameter of the food-grade plastic bottle is D3, and the height is X2; the amount of artificial feed of the forked-horned bugs added to the 2nd-instar nymphs is M1, the amount of artificial feed of the forked-horned bugs added to the 3rd-instar nymphs is M2, the amount of artificial feed of the forked-horned bugs added to the 4th-instar nymphs is M3, and the amount of artificial feed of the forked-horned bugs added to the 5th-instar nymphs is M4; The method of feeding honey water includes sticking a cotton block containing honey water with a mass concentration of 8% to 12% on the inner box wall of a cylindrical box; The method for adding artificial feed to the forked-horned bug includes cutting the nanosponge into 40mm×40mm squares as a food table, digging a circular hole with a diameter of 20mm in the middle, then providing a 2cm aluminum foil and folding it into a disc shape and placing it in the circular hole, and then adding the prepared artificial feed to the forked-horned bug on the aluminum foil in the circular hole.

10. The method for raising Pseudocerma sutchuenensis according to claim 9, characterized in that: ρ1 is 90 to 110 heads / box; and / or T3 is 26℃~28℃; and / or RH2 is 65%~75%; and / or L2: D'2 is 16:8; and / or V is 1L~1.5L; and / or D3 is 10cm~12cm; and / or X2 is 14cm~16cm; and / or M1: 100 heads / 90 mg~110 mg; and / or M2: 100 heads / 190 mg~210 mg; and / or M3: 100 heads / 700 mg~900 mg; and / or M4 is 100 heads / 1500 mg~1700 mg.

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