Royal jelly capsule containing L-carnitine and preparation method thereof
The fishy smell and royal jelly sensitization through enzyme immobilization technology and the fishy smell of adsorbents are used to solve the problems of fishy smell and royal jelly sensitization of L-carnitine products, and the preparation of low-allergic and palatable royal jelly capsules is achieved, improving the application field and consumption experience of the product.
Patent Information
- Application Number
- CN202510246073.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-04
- Publication Date
- 2025-04-18
AI Technical Summary
The existing L-carnitine products have fishy smell problems, which affects their palatability and application fields, and the allergen and glycosyl groups in royal jelly lead to adverse reactions.
Deglycosylated royal jelly through enzyme immobilization technology to reduce its sensitization, and use adsorbent to remove the trimethylamine odor in L-carnitine tartrate to prepare hypoallergenic and palatable royal jelly capsules.
It effectively reduces the sensitization and adverse reaction probability of royal jelly capsules, improves the palatability and consumption experience of the product, and expands its application scope in the field of health foods.
Smart Images
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Figure HDA0005295531930000012
Abstract
Description
Technical Field
[0001] The invention belongs to the field of health food, and particularly relates to a royal jelly capsule containing L-carnitine and a preparation method thereof. Background Art
[0002] Royal jelly has attracted much attention as an ideal natural health product. Royal jelly usually contains 12-15% crude protein. Royal jelly protein is mainly composed of water-soluble protein and water-insoluble protein. The water-soluble protein accounting for 46-98% of the total protein content is called royal jelly main protein (MRJPs). MRJP1-MRJP99 are the components of royal jelly main protein. Among them, MRJP1, the most abundant protein, can exert immune and pro-inflammatory activities. MRJP2, which accounts for 16% of the total royal jelly protein, is also the main allergen of royal jelly. Early studies have found that carbohydrates on allergens can cause specific T cell responses, and sugar groups may play a vital role in allergic reactions induced by allergens. Removing the sugar groups of allergens can significantly increase the level of specific IgE.
[0003] Trimethylamine is a typical volatile organic compound. After entering the human body through food, trimethylamine can be converted into carcinogenic N-nitrosamine compounds, which will bring health risks to the human body. The chemical synthesis method of L-carnitine inevitably uses the key raw material trimethylamine. Because trimethylamine has a strong fishy smell, the synthesized L-carnitine and the subsequent synthesized L-carnitine tartrate have a strong fishy smell. Even if it is made into capsules, it can improve compliance when taken, but the stomach gas after taking it will still affect the taking experience, which seriously affects the application of L-carnitine products in nutrition and health care, food additives and feed additives. Summary of the invention
[0004] The present invention mainly provides a low-sensitivity and good-palatability royal jelly capsule containing L-carnitine, and a preparation method of the capsule. The technical scheme is as follows:
[0005] A royal jelly capsule containing L-carnitine comprises, by weight, 100-150 parts of L-carnitine tartrate, 100-120 parts of royal jelly freeze-dried powder, 80-110 parts of rhodiola rosea extract and 40-70 parts of American ginseng extract.
[0006] Further, the royal jelly freeze-dried powder is low-sensitivity royal jelly; an enzyme immobilization carrier is prepared, a deglycosylation enzyme is immobilized, and then the royal jelly is deglycosylated to obtain low-sensitivity royal jelly; the L-carnitine tartrate is deodorized L-carnitine tartrate; an adsorbent is used to encapsulate L-carnitine tartrate, so that L-carnitine tartrate is fully contacted with the adsorbent, and an adsorption treatment is performed to remove the fishy smell, and deodorized L-carnitine tartrate is obtained. The deglycosylation enzyme uses PNGaseF enzyme.
[0007] Furthermore, the preparation of the low-sensitivity royal jelly comprises the following steps:
[0008] a. Dissolve acrylic acid and acrylamide in water, then add an initiator to the solution, react for 3 to 5 hours at 30 to 35°C in a nitrogen atmosphere to gel; freeze the obtained gel until the core temperature is below -18°C, melt it at room temperature, place it in water, precipitate and separate it several times to remove any unreacted monomers; freeze-dry it to obtain the carrier;
[0009] b. Dispersing magnetic particles in water, adding branched polyethyleneimine and mixing evenly to obtain a magnetic dispersion; placing the deglycosylation enzyme in a buffer solution to prepare an enzyme solution; placing the carrier in the enzyme solution, fully dispersing it, and then adding the magnetic dispersion to obtain a mixed solution; adding glutaraldehyde to the mixed solution and reacting it on a shaking table; separating and collecting the precipitate, and fully washing it with a buffer solution to obtain an immobilized enzyme;
[0010] c. Dissolve the freeze-dried royal jelly powder in ammonium bicarbonate solution, then add the immobilized enzyme, and react on a shaking table at 30-38°C for 6-16 hours; separate the immobilized enzyme by magnetic attraction, and then freeze-dry the remaining product to obtain low-sensitivity royal jelly.
[0011] Furthermore, the mass ratio of acrylic acid to acrylamide in step a is 1:4-5; the initiator in step a is ammonium persulfate and sodium bisulfite; and the initiator accounts for 0.2-0.5% of the total mass of the monomers.
[0012] Furthermore, the mass ratio of the branched polyethyleneimine in step b to the carrier is 1:1.5-3; the mass ratio of the magnetic particles in step b to the carrier is 1:1.2-2; the concentration of the carrier in the system in step b is 0.05-0.3 g / mL; the concentration of glutaraldehyde in the system in step b is 0.05-0.3 g / mL.
[0013] Furthermore, the enzyme solution in step b is prepared by placing the deglycosylation enzyme in a 50 mmol / L, pH 7.0 phosphate buffer to prepare an enzyme solution with a concentration of 500 to 1000 U / g.
[0014] Furthermore, the shaking reaction in step b is carried out at 20-35° C. and 100-200 rpm for 4-8 hours.
[0015] Furthermore, the preparation of the adsorbent includes the following steps: dissolving sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; adding chitosan to the sodium starch octenyl succinate solution and fully dispersing it, and dripping acetic acid until the solution is clarified; continuing to add ferric sulfate and fully mixing it, and reacting it at 140-160° C. for 8-12 hours; after cooling to room temperature, adding glutaraldehyde solution, pouring it into a mold and keeping it horizontal, drying it, washing it with water, and drying it again to obtain the adsorbent.
[0016] Furthermore, the mass ratio of the sodium starch octenylsuccinate to chitosan is 1:0.2-0.5; the mass ratio of the chitosan to ferrous sulfate is 3-8:1; and the mass ratio of the chitosan to glutaraldehyde is 1:0.02-0.08.
[0017] A method for preparing the above-mentioned royal jelly capsule containing L-carnitine comprises the following steps: under sterile conditions, sieving L-carnitine tartrate, royal jelly freeze-dried powder, rhodiola rosea extract and American ginseng extract, sieving the raw materials according to the formula and mixing them, and then filling them into hollow capsules with a disintegration time of no more than 60 minutes.
[0018] The adsorbent after adsorption is placed in an oven at 35°C for heating and desorption to obtain a regenerated adsorbent, which is reused in the next adsorption.
[0019] By adopting the above scheme, the method of the present invention has the following advantages:
[0020] 1. The present invention uses enzymes to deglycosylate royal jelly, thereby reducing the ability of proteins to bind to antibodies, reducing the sensitization rate of glycoproteins in royal jelly, reducing the probability of adverse reactions caused by the product, and making the application of the royal jelly capsule of the present invention wider.
[0021] 2. The present invention loads the enzyme and magnetic microbeads together on a polymer carrier, which can achieve enzyme immobilization and convenient recovery, improve enzymolysis efficiency, reduce enzyme loss, and reduce production costs.
[0022] 3. After freeze drying, the carrier of the present invention removes water molecules between polymers, making the carrier structure loose, and under the action of branched polyethyleneimine, the enzyme and magnetic microbeads can more easily enter the interior of the carrier, thereby achieving firm loading.
[0023] 4. The present invention uses branched polyethyleneimine to cross-link the carrier and the enzyme through the amino group to reduce the release of the enzyme, and forms electrostatic adsorption with the magnetic microbeads to connect the carrier, enzyme and magnetic microbeads into a whole.
[0024] 5. The adsorbent of the present invention combines chitosan, which has a strong affinity for amine gases, and iron oxide with a high surface area to achieve the adsorption of low-concentration trimethylamine, and treats L-carnitine tartrate products to fully remove residual trimethylamine and improve the palatability of the product.
[0025] 6. The adsorbent of the present invention prepares iron oxide in situ in chitosan, and adds sodium starch octenyl succinate to improve the dispersibility of the generated iron oxide in the system, promote the miniaturization of the iron oxide particle size, and make the final film formation more uniform and complete, thereby improving the adsorption effect.
[0026] 7. The preparation method of the present invention is simple, the raw material processing cost is low, the immobilized enzyme and adsorbent used for enzymolysis can be reused, the production cost is low, and the obtained royal jelly capsule containing L-carnitine has few adverse reactions, no obvious odor will be produced when gastric gas is reversed, and the taking satisfaction is high. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 is a comparison chart of SIgE levels in the serum of mice in each group;
[0028] Figure 2 This is a comparison chart of SIgG2a levels in the serum of each group of mice. DETAILED DESCRIPTION
[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0030] Embodiment 1:
[0031] (1) 1 g of acrylic acid and 4 g of acrylamide are dissolved in water, and then 10 mg of ammonium persulfate and 5 mg of sodium bisulfite are added to the solution. The mixture is reacted for 5 h at 30-35° C. in a nitrogen atmosphere to form a gel. The obtained gel is frozen to a core temperature below −18° C., melted at room temperature, and placed in water for multiple precipitations and separations to remove any unreacted monomers. After freeze-drying, the carrier is obtained.
[0032] (2) Dispersing 2 g of magnetic microparticles in 10 mL of water, adding 1 g of branched polyethyleneimine and mixing well to obtain a magnetic dispersion; placing the enzyme in 10 mL of 50 mmol / L, pH 7.0 phosphate buffer to prepare an enzyme solution with a concentration of 800 U / g; taking 3 g of the carrier and placing it in the enzyme solution, fully dispersing it, and then adding the magnetic dispersion to obtain a mixed solution; adding 0.04 mL of glutaraldehyde to the mixed solution, and shaking the mixture at 30°C and 100-200 rpm for 8 h; separating and collecting the precipitate, and washing it thoroughly with a buffer to obtain an immobilized enzyme;
[0033] (3) dissolving the freeze-dried royal jelly powder in an ammonium bicarbonate solution, then adding an immobilized enzyme of the same weight as the freeze-dried royal jelly powder, and reacting on a shaking table at 30 to 38° C. for 12 hours; separating the immobilized enzyme by magnetic attraction, and then freeze-drying the remaining product to obtain low-sensitivity royal jelly;
[0034] (4) Dissolve 5 g of sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; add 2.5 g of chitosan to the sodium starch octenyl succinate solution and fully disperse it, and drop acetic acid until the solution is clear; continue to add 0.5 g of ferric sulfate and mix thoroughly, and react at 140° C. for 12 h; after cooling to room temperature, add 0.02 mL of glutaraldehyde, pour it into a mold and keep it horizontal, dry it, soak it in water several times, wash it thoroughly, and dry it again to obtain an adsorbent;
[0035] (5) L-carnitine tartrate is coated with an adsorbent and fully shaken at 30-40° C. for 0.5-2 h to allow the L-carnitine tartrate to fully contact with the adsorbent for adsorption treatment to remove the fishy smell, thereby obtaining deodorized L-carnitine tartrate.
[0036] Embodiment 2: The difference from Embodiment 1 is that:
[0037] (2) Dispersing 2 g of magnetic microparticles in 10 mL of water, adding 1 g of branched polyethyleneimine and mixing well to obtain a magnetic dispersion; placing the enzyme in 10 mL of 50 mmol / L, pH 7.0 phosphate buffer to prepare an enzyme solution with a concentration of 500 U / g; taking 3 g of the carrier and placing it in the enzyme solution, fully dispersing it, and then adding the magnetic dispersion to obtain a mixed solution; adding 0.04 mL of glutaraldehyde to the mixed solution, and shaking the mixture at 30°C and 100-200 rpm for 8 hours; separating and collecting the precipitate, and washing it thoroughly with a buffer to obtain an immobilized enzyme.
[0038] Embodiment 3: The difference from Embodiment 1 is that:
[0039] (2) Dispersing 2 g of magnetic microparticles in 10 mL of water, adding 1 g of branched polyethyleneimine and mixing well to obtain a magnetic dispersion; placing the enzyme in 10 mL of 50 mmol / L, pH 7.0 phosphate buffer to prepare an enzyme solution with a concentration of 800 U / g; taking 1.6 g of the carrier and placing it in the enzyme solution, fully dispersing it, and then adding the magnetic dispersion to obtain a mixed solution; adding 0.04 mL of glutaraldehyde to the mixed solution, and shaking the mixture at 30°C and 100-200 rpm for 8 hours; separating and collecting the precipitate, and fully washing it with a buffer to obtain an immobilized enzyme.
[0040] Embodiment 4: The difference from Embodiment 1 is that:
[0041] (2) Dispersing 2 g of magnetic microparticles in 10 mL of water, adding 1 g of branched polyethyleneimine and mixing well to obtain a magnetic dispersion; placing the enzyme in 10 mL of 50 mmol / L, pH 7.0 phosphate buffer to prepare an enzyme solution with a concentration of 800 U / g; taking 3 g of the carrier and placing it in the enzyme solution, fully dispersing it, and then adding the magnetic dispersion to obtain a mixed solution; adding 0.04 mL of glutaraldehyde to the mixed solution, and shaking the mixture at 30°C and 100-200 rpm for 4 hours; separating and collecting the precipitate, and washing it thoroughly with a buffer to obtain an immobilized enzyme.
[0042] Embodiment 5: The difference from Embodiment 1 is that:
[0043] (3) Dissolving the freeze-dried royal jelly powder in an ammonium bicarbonate solution, then adding an immobilized enzyme in an amount equal to the weight of the freeze-dried royal jelly powder, and reacting on a shaking table at 30-38° C. for 16 h; separating the immobilized enzyme by magnetic attraction, and then freeze-drying the remaining product to obtain low-sensitivity royal jelly.
[0044] Embodiment 6: The difference from Embodiment 1 is that:
[0045] (3) Dissolving the freeze-dried royal jelly powder in an ammonium bicarbonate solution, then adding an immobilized enzyme in an amount equal to the weight of the freeze-dried royal jelly powder, and reacting on a shaking table at 30-38° C. for 6 h; separating the immobilized enzyme by magnetic attraction, and then freeze-drying the remaining product to obtain a low-sensitivity royal jelly.
[0046] Embodiment 7: The difference from Embodiment 1 is that:
[0047] (4) Dissolve 5 g of sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; add 1 g of chitosan to the sodium starch octenyl succinate solution and fully disperse it, and drop acetic acid until the solution is clear; continue to add 0.5 g of ferric sulfate and mix thoroughly, and react at 140° C. for 12 h; after cooling to room temperature, add 0.02 mL of glutaraldehyde, pour it into a mold and keep it horizontal, soak it in water several times after drying, wash it thoroughly, and dry it again to obtain an adsorbent.
[0048] Embodiment 8: The difference from Embodiment 1 is that:
[0049] (4) Dissolve 5 g of sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; add 2.5 g of chitosan to the sodium starch octenyl succinate solution and fully disperse it, and drop acetic acid until the solution is clear; continue to add 0.2 g of ferric sulfate and mix thoroughly, and react at 140° C. for 12 h; after cooling to room temperature, add 0.02 mL of glutaraldehyde, pour it into a mold and keep it horizontal, soak it in water several times after drying, wash it thoroughly, and dry it again to obtain an adsorbent.
[0050] Embodiment 9: The difference from Embodiment 1 is that:
[0051] (4) Dissolve 5 g of sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; add 2.5 g of chitosan to the sodium starch octenyl succinate solution and fully disperse it, and drop acetic acid until the solution is clear; continue to add 0.5 g of ferric sulfate and mix thoroughly, and react at 160° C. for 12 h; after cooling to room temperature, add 0.02 mL of glutaraldehyde, pour it into a mold and keep it horizontal, soak it in water several times after drying, wash it thoroughly, and dry it again to obtain an adsorbent.
[0052] Embodiment 10: The difference from Embodiment 1 is that:
[0053] (4) Dissolve 5 g of sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; add 2.5 g of chitosan to the sodium starch octenyl succinate solution and fully disperse it, and drop acetic acid until the solution is clear; continue to add 0.5 g of ferric sulfate and mix thoroughly, and react at 140° C. for 8 h; after cooling to room temperature, add 0.02 mL of glutaraldehyde, pour it into a mold and keep it horizontal, soak it in water several times after drying, wash it thoroughly, and dry it again to obtain an adsorbent.
[0054] Comparative Example 1: The difference from Example 1 is that untreated royal jelly freeze-dried powder is used.
[0055] Comparative Example 2: L-carnitine tartrate without deodorization treatment was used.
[0056] The samples obtained in each embodiment are made into capsules according to the following method:
[0057] Under sterile conditions, the raw materials are sieved and mixed in a total mixer according to a formula of 1.3g of deodorized L-carnitine tartrate, 1.15g of low-sensitivity royal jelly, 0.95g of Rhodiola rosea extract and 0.6g of American ginseng extract, and then filled into 400mg / capsule hollow capsules with a disintegration time of no more than 60min to prepare royal jelly capsules containing L-carnitine.
[0058] Preparation and testing of samples of the embodiments:
[0059] The purchased BALB / c mice were randomly divided into cages and raised, with 5 mice in each cage, and 2 cages of mice in 1 group: Comparative Example 1 and Example 1 to 6 were set according to the recommended oral dosage for humans, and a blank control group was set at the same time. The sample contents were weighed, dispersed with water, and a 20 mg / mL dispersion was prepared. The mice were gavaged at a dose of 1.4 mL / kg, twice a day, 3 days apart, for a total of 12 times, and gavage was stimulated 3 times. After stimulation, the mouse serum was collected to measure: sIgE and sIgG2a.
[0060] Depend on Figure 1 and Figure 2 It can be seen that the slgE and sIgG2a levels of Comparative Example 1, which is not deglycosylated, are the highest, and the slgE and sIgG2a levels of each embodiment are significantly lower. Compared with Example 1, the enzyme concentration loaded in Example 2 is low, the enzyme loaded is less, the hydrolysis effect is not as good as Example 1, and the slgE and sIgG2a levels are higher. The carrier of Example 3 is less, the content of magnetic particles, branched polyethyleneimine and enzyme to be loaded is relatively increased, the enzymolysis effect is better, slgE and sIgG2a decrease but are close to the limit of reducing the sensitization of royal jelly. The enzyme immobilization time of Example 4 is shorter, and the levels of slgE and sIgG2a are significantly increased, indicating that longer time is conducive to the fixation of enzymes. The enzyme treatment time of Example 5 is longer, and the enzyme treatment time of Example 6 is shorter, and the levels of slgE and sIgG2a of Example 6 are significantly increased, indicating that the degree of deglycosylation treatment using enzymes is related to the levels of slgE and sIgG2a, and removing sugar groups can significantly reduce the allergic reactions mediated by slgE and sIgG2a.
[0061] The capsules prepared in Example 1, Examples 7 to 10 and Comparative Example 2 were disassembled and the contents were taken out. They were placed on evaporating dishes respectively and 10 testers aged 25 to 45 without respiratory diseases were asked to heat the samples to 40 to 50°C and smell them to evaluate the degree of fishy smell. The samples were ranked from heavy to light in terms of fishy smell, with the first one getting one point and the second one getting two points, and the scores of each sample were summed up to evaluate the palatability of the capsules. The higher the score, the better the palatability.
[0062] The results are as follows:
[0063] Points Example 1 58 Example 7 23 Example 8 31 Example 9 46 Example 10 42 Comparative Example 2 10
[0064] As can be seen from the above table, the testers unanimously gave the lowest evaluation to Comparative Example 2, indicating that the palatability of each embodiment is significantly better than that of Comparative Example 2, which has not undergone deodorization treatment. Among them, Example 1, which has not undergone chitosan and iron reduction, has the highest score, while the scores of Example 7 and Example 8, which have reduced chitosan, are significantly reduced, and the score of Example 7, which has reduced chitosan, is even lower. It may be that chitosan not only participates in the adsorption of trimethylamine, but is also related to the adsorption performance of the adsorbent membrane matrix itself, and the reduction of chitosan has a significant effect on the adsorption of trimethylamine.
[0065] For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the protection scope of the claims of the present invention.
Claims
1. A royal jelly capsule containing L-carnitine, characterized in that: The invention comprises, by weight, 100 to 150 parts of L-carnitine tartrate, 100 to 120 parts of freeze-dried royal jelly powder, 80 to 110 parts of rhodiola rosea extract and 40 to 70 parts of American ginseng extract.
2. The royal jelly capsule containing L-carnitine according to claim 1, characterized in that: The royal jelly freeze-dried powder is low-sensitivity royal jelly; an enzyme fixing carrier is prepared to fix the deglycosylation enzyme, and then the royal jelly is deglycosylated to obtain the low-sensitivity royal jelly; the L-carnitine tartrate is deodorized L-carnitine tartrate; an adsorbent is used to encapsulate the L-carnitine tartrate, so that the L-carnitine tartrate is in full contact with the adsorbent, and an adsorption treatment is performed to remove the fishy smell, so as to obtain the deodorized L-carnitine tartrate.
3. The royal jelly capsule containing L-carnitine according to claim 1, characterized in that: The preparation of the hypoallergenic royal jelly comprises the following steps: a. Dissolve acrylic acid and acrylamide in water, then add an initiator to the solution, react for 3 to 5 hours at 30 to 35°C in a nitrogen atmosphere to gel; freeze the obtained gel until the core temperature is below -18°C, melt it at room temperature, place it in water, precipitate and separate it several times to remove any unreacted monomers; freeze-dry it to obtain the carrier; b. Dispersing magnetic particles in water, adding branched polyethyleneimine and mixing evenly to obtain a magnetic dispersion; placing the deglycosylation enzyme in a buffer solution to prepare an enzyme solution; placing the carrier in the enzyme solution, fully dispersing it, and then adding the magnetic dispersion to obtain a mixed solution; adding glutaraldehyde to the mixed solution and reacting it on a shaking table; separating and collecting the precipitate, and fully washing it with a buffer solution to obtain an immobilized enzyme; c. Dissolve the freeze-dried royal jelly powder in ammonium bicarbonate solution, then add the immobilized enzyme, and react on a shaking table at 30-38°C for 6-16 hours; separate the immobilized enzyme by magnetic attraction, and then freeze-dry the remaining product to obtain low-sensitivity royal jelly.
4. The royal jelly capsule containing L-carnitine according to claim 3, characterized in that: The mass ratio of acrylic acid to acrylamide in step a is 1:4-5; the initiator in step a is ammonium persulfate and sodium bisulfite; the initiator accounts for 0.2-0.5% of the total mass of the monomers.
5. The royal jelly capsule containing L-carnitine according to claim 3, characterized in that: The mass ratio of the branched polyethyleneimine to the carrier in step b is 1:1.5-3; the mass ratio of the magnetic particles to the carrier in step b is 1:1.2-2; the concentration of the carrier in the system in step b is 0.05-0.3 g / mL; the concentration of glutaraldehyde in the system in step b is 0.05-0.3 g / mL.
6. The royal jelly capsule containing L-carnitine according to claim 3, characterized in that: The preparation of the enzyme solution in step b is to place the deglycosylation enzyme in a 50 mmol / L, pH 7.0 phosphate buffer to prepare an enzyme solution with a concentration of 500 to 1000 U / g.
7. The royal jelly capsule containing L-carnitine according to claim 3, characterized in that: The shaking reaction in step b is carried out at 20-35° C. and 100-200 rpm for 4-8 hours.
8. The royal jelly capsule containing L-carnitine according to claim 1, characterized in that: The preparation of the adsorbent comprises the following steps: Dissolve sodium starch octenyl succinate in water to obtain a sodium starch octenyl succinate solution; add chitosan to the sodium starch octenyl succinate solution and fully disperse it, and drop acetic acid until the solution is clarified; continue to add ferric sulfate and mix thoroughly, and react at 140-160° C. for 8-12 hours; add glutaraldehyde solution after cooling to room temperature, pour it into a mold and keep it horizontal, wash it with water after drying, and dry it again to obtain an adsorbent.
9. The royal jelly capsule containing L-carnitine according to claim 8, characterized in that: The mass ratio of sodium starch octenylsuccinate to chitosan is 1:0.2-0.5; the mass ratio of chitosan to ferrous sulfate is 3-8:1; the mass ratio of chitosan to glutaraldehyde is 1:0.02-0.
08.
10. A method for preparing royal jelly capsules containing L-carnitine according to claim 1, characterized in that the steps include: Under sterile conditions, L-carnitine tartrate, royal jelly freeze-dried powder, rhodiola rosea extract and American ginseng extract are sieved, the raw materials are sieved according to the formula and then mixed, and then filled into hollow capsules with a disintegration time of no more than 60 minutes.