Hydrogel fiber composition with weight-losing effect as well as preparation method and application of hydrogel fiber composition

Abstract

The invention belongs to the field of weight losing, and particularly relates to a hydrogel fiber composition with a weight losing effect as well as a preparation method and application thereof. The white kidney bean extract, the dendrobium officinale extract, the rhizoma polygonati extract, the microcos paniculata extract, the dark plum extract, the structural fat, the fructo-oligosaccharide, the maltodextrin and the lactobacillus acidophilus powder are added into the edible hydrogel cellulose powder, so that the weight-losing effect of the hydrogel cellulose powder can be effectively improved, and nutritional ingredients can be increased; the defect of insufficient nutritional ingredients of the hydrogel cellulose powder is overcome, the weight losing requirement of people can be met, the supply of necessary nutrients for the body is ensured, and the safety and effectiveness of weight losing products are greatly improved.

Description

Technical Field

[0001] The invention belongs to the field of weight loss, and in particular relates to a hydrogel fiber composition with weight loss effect, a preparation method and application thereof. Background Art

[0002] Obesity does not simply refer to weight gain, but rather to the state of excessive accumulation of fat tissue in the body. Excessive food intake is an important cause of weight gain and accumulation of fat tissue in the body. Therefore, properly controlling food intake is one of the important ways to lose weight. Summary of the invention

[0003] The invention provides a hydrogel fiber composition with weight-loss effect, a preparation method and application thereof, which can increase satiety, effectively reduce food intake and facilitate weight loss.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A hydrogel fiber composition with weight loss effect comprises the following components in parts by weight: 15-25 parts of hydrogel cellulose powder, 1-5 parts of white bean extract, 1-5 parts of Dendrobium officinale extract, 1-3 parts of Polygonatum sibiricum extract, 0.5-1.5 parts of Buza leaf extract, 0.3-0.7 parts of Wumei extract, 1-10 parts of structural fat, 1-10 parts of oligofructose, 5-15 parts of maltodextrin, 1-5 parts of Lactobacillus acidophilus powder, and 20 mg of sodium hyaluronate is added to every 2 g of the above components in total.

[0006] Furthermore, the composition comprises the following components in parts by weight: 20 parts of hydrogel cellulose powder, 3 parts of white bean extract, 3 parts of Dendrobium officinale extract, 2 parts of Polygonatum sibiricum extract, 1 part of Buzha leaf extract, 0.5 parts of Wumei extract, 5 parts of structural fat, 5 parts of oligofructose, 10 parts of maltodextrin, and 3 parts of Lactobacillus acidophilus powder.

[0007] Furthermore, the preparation method of the Buzha leaf extract is: take Buzha leaves, add water to boil, filter to obtain a filtrate, and dry the filtrate; the preparation method of the black plum extract is: take black plum, add water to boil, filter to obtain a filtrate, and dry the filtrate.

[0008] Furthermore, the amount of water added is 6-8 times the total mass of the Buza leaves or Wumei, the decocting time is 20-30 minutes, and the drying method is vacuum freeze drying.

[0009] Further, the preparation method of the Lactobacillus acidophilus powder comprises the following steps:

[0010] (1) Disinfection of the fermentation tank, followed by addition of culture medium and sterilization;

[0011] (2) inoculating pure culture strains of Lactobacillus acidophilus into a fermentation tank medium for fermentation;

[0012] (3) After fermentation, the fermentation liquid is spray-dried to obtain Lactobacillus acidophilus powder.

[0013] The preparation method of the above-mentioned composition comprises the following steps:

[0014] Take the formulated amounts of hydrogel cellulose powder, white kidney bean extract, dendrobium officinale extract, polygonatum sibiricum extract, buzia leaf extract, ebony extract, structural fat, oligofructose, maltodextrin and lactobacillus acidophilus powder and mix them evenly.

[0015] A hydrogel fiber solid beverage for weight loss comprises the above composition.

[0016] The preparation method of the solid beverage comprises the following steps: taking the above composition, crushing it and passing it through a 20-60 mesh sieve to obtain a solid beverage.

[0017] Application of the above-mentioned composition or solid beverage in the preparation of weight loss preparations.

[0018] Furthermore, in the application, the method of consumption is: take the composition or solid beverage, add warm water not exceeding 45°C and drink it, and add 200 ml of warm water to every 2 g of the composition.

[0019] Furthermore, the composition or solid beverage is consumed 4-10 times a day, 2 g each time.

[0020] Beneficial effects of the present invention:

[0021] Edible hydrogel is a new type of weight loss product, but it lacks necessary nutrients and is difficult to achieve a good weight loss effect. The present invention adds white kidney bean extract, dendrobium candidum extract, polygonatum extract, buzia leaf extract, ebony extract, structural fat, oligofructose, maltodextrin, and lactobacillus acidophilus powder to edible hydrogel cellulose powder, which can not only effectively improve the weight loss effect of hydrogel cellulose powder, but also increase the nutrients to make up for the defect of insufficient nutrients in hydrogel cellulose powder, which can not only meet people's requirements for weight loss, but also ensure the supply of necessary nutrients for the body, and greatly improve the safety and effectiveness of weight loss products. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 These are the experimental results of promoting fat metabolism in zebrafish.

[0023] Figure 2 These are the results of the zebrafish fat absorption inhibition experiment. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] In the present invention, the hydrogel cellulose powder is an edible hydrogel cellulose powder produced by Guangzhou Hualiang Light Biotechnology Co., Ltd., with a production batch number of 2025010801, which meets the GB / T29602-2013 solid beverage standard; white kidney bean extract and Dendrobium candidum extract are purchased from Shanxi Nanba Biotechnology Co., Ltd., with production batch numbers of 2024101401 and 2024100907, respectively; Polygonatum sibiricum extract is purchased from Xuancheng Baicao Pharmaceutical Co., Ltd., with a production batch number of 20241213; Sodium hyaluronate is purchased from Shanxi Nanba Biotechnology Co., Ltd., with a production batch number of 2024101001; Fructooligosaccharide is purchased from Quantum Hi-Tech (Guangdong) Biological Co., Ltd., with a production batch number of 2024.03.10. Lactobacillus acidophilus is obtained by fermentation of Lactobacillus acidophilus, and the Lactobacillus acidophilus strain is purchased from the General Microbiological Center of the China Microbiological Culture Collection Administration, with a strain number of 1.1854.

[0026] Example 1

[0027] A hydrogel fiber composition with a weight loss effect comprises the following components in parts by weight: 15 parts of hydrogel cellulose powder, 1 part of white bean extract, 1 part of Dendrobium officinale extract, 1 part of Polygonatum sibiricum extract, 0.5 parts of Buza leaf extract, 0.3 parts of Prunus mume extract, 1 part of structural fat, 1 part of oligofructose, 5 parts of maltodextrin, 1 part of Lactobacillus acidophilus powder, and 20 mg of sodium hyaluronate is added for every 2 g of the above components.

[0028] The preparation method of the composition comprises the following steps:

[0029] Take the formulated amounts of hydrogel cellulose powder, white kidney bean extract, dendrobium officinale extract, polygonatum sibiricum extract, buzia leaf extract, ebony extract, structural fat, oligofructose, maltodextrin and lactobacillus acidophilus powder and mix them evenly.

[0030] The preparation method of the Buzha leaf extract is as follows: taking Buzha leaves, adding water to boil, filtering to obtain a filtrate, and drying the filtrate; the preparation method of the black plum extract is as follows: taking black plums, adding water to boil, filtering to obtain a filtrate, and drying the filtrate.

[0031] The amount of water added is 6-8 times the total mass of Buzha leaves or Wumei, the decocting time is 20-30 minutes, and the drying method is vacuum freeze drying.

[0032] Example 2

[0033] A hydrogel fiber composition with a weight loss effect, the composition comprising the following components in parts by weight: 20 parts of hydrogel cellulose powder, 3 parts of white bean extract, 3 parts of Dendrobium officinale extract, 2 parts of Polygonatum sibiricum extract, 1 part of Buza leaf extract, 0.5 parts of Prunus mume extract, 5 parts of structural fat, 5 parts of oligofructose, 10 parts of maltodextrin, 3 parts of Lactobacillus acidophilus powder, and 20 mg of sodium hyaluronate is added for every 2 g of the above components.

[0034] The preparation method is the same as that in Example 1.

[0035] Example 3

[0036] A hydrogel fiber composition with a weight loss effect comprises the following components in parts by weight: 25 parts of hydrogel cellulose powder, 5 parts of white bean extract, 5 parts of Dendrobium officinale extract, 3 parts of Polygonatum sibiricum extract, 1.5 parts of Buza leaf extract, 0.7 parts of Prunus mume extract, 10 parts of structural fat, 10 parts of oligofructose, 15 parts of maltodextrin, and 5 parts of Lactobacillus acidophilus powder. According to the total amount of the above components, 20 mg of sodium hyaluronate is added for every 2 g.

[0037] The preparation method is the same as that in Example 1.

[0038] In the above embodiments, the preparation method of the Buzha leaf extract is: taking Buzha leaves, boiling them with water, filtering them to obtain a filtrate, and drying the filtrate; the preparation method of the black plum extract is: taking black plums, boiling them with water, filtering them to obtain a filtrate, and drying the filtrate.

[0039] The preparation method of Lactobacillus acidophilus powder comprises the following steps:

[0040] The Lactobacillus acidophilus strain was inoculated into MRS medium and cultured at 37°C for 48 hours to expand the culture and obtain a pure culture strain.

[0041] The fermentation tank is sterilized at a pressure of 0.1Mpa, a temperature of 121°C, and a time of 30-40 minutes, after which the culture medium is added, the tank pressure is maintained at 0.08Mpa, the temperature is 105°C, and the sterilization time is 20 minutes; the composition ratio of the culture medium material is 40% skim milk powder, 40% glucose, and 75% water of the total mass of the material is added.

[0042] The pure culture strain is inoculated into the fermentation tank culture medium at an inoculation rate of 500 ml of pure culture strain per 11 kg of culture medium, and constant temperature culture and fermentation are carried out (the tank pressure is maintained at 0.02Mpa-0.10Mpa, the time is 28 hours, and the pH is adjusted to 5.5-6.0).

[0043] After the culture, the fermentation liquid is spray-dried (spraying starts when the inlet air temperature is 145-150°C, and the outlet air temperature is 85-90°C) to obtain Lactobacillus acidophilus powder (inactive probiotics).

[0044] Example 4

[0045] A hydrogel fiber solid beverage for weight loss comprises the above composition.

[0046] The preparation method is as follows: the above composition is taken, crushed and passed through a 20-60 mesh sieve to obtain a solid beverage.

[0047] The method for taking the prepared solid beverage is as follows: add 200 ml of warm water not exceeding 45° C. to every 2 g of the composition and take the solid beverage 4 to 10 times a day, 2 g each time.

[0048] Comparison of weight loss effects of the composition of the present invention

[0049] Compositions of different compositions were prepared according to the ratios described in Table 1, and then passed through a 60-mesh sieve to obtain solid beverages of different compositions for effect evaluation.

[0050] Table 1 Composition of different solid beverages

[0051]

[0052]

[0053] Based on the daily intake of 8g for a person with a body weight of 70kg, the test concentration is 15 times the daily intake. The solid beverages prepared above were respectively taken and added with fish embryo culture medium to prepare solid beverage solutions with a concentration of 1.71g / L.

[0054] The preparation method of fish embryo culture medium is as follows: weigh 2940 mg of anhydrous calcium chloride, 1233 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, and 55 mg of potassium chloride and dissolve them in 10 L of water. The pH value is 6.5 to 8.5. All chemicals are of analytical grade.

[0055] (1) Weight loss effect (zebrafish fat metabolism promotion test method)

[0056] The high-fat diet-induced obesity model was used for testing.

[0057] Healthy zebrafish embryos were selected and cultured at 28°C ± 1°C until 5 days after fertilization at a density of no more than 1 fish embryo in 200 μL fish embryo culture medium. Five-day-old zebrafish were exposed to 1% egg yolk solution for 24 hours to induce an obesity model, and 30 zebrafish were randomly selected for each treatment. The solid beverage group was added with 6 mL of solid beverage sample solution with a concentration of 1.71 g / L, and a model control group, a blank control group, and a positive control group were set up at the same time. The blank control group was added with 6 mL of fish embryo culture medium, the model control group was added with 6 mL of 1% egg yolk solution (1% egg yolk solution was freshly prepared with fish embryo culture medium), and the positive control group was added with 6 mL of fish embryo culture medium containing 0.3 μM ezetimibe (a certain amount of ezetimibe was dissolved in dimethyl sulfoxide to prepare a storage solution with a concentration of 3mM, and then the storage solution was diluted to 0.3 μM with fish embryo culture medium, and freshly prepared before use). Each treatment group was placed in a 28°C ± 1°C incubator for 24h ± 1h. At least 90% of the zebrafish in each treatment group survived after culture, otherwise the results of the corresponding test group were invalid.

[0058] After fixing each test group of zebrafish in paraformaldehyde for at least 1 hour, treat the zebrafish with PBST for 2 minutes, repeat 3 times. Treat the zebrafish with bleach solution for 10 minutes. If treating in a test tube, keep the lid open. Treat with PBST for 2 minutes, then treat with 60% 2-propanol solution for 5 minutes. Stain with Oil Red O solution for 75 minutes ± 10 minutes. Immerse in 60% 2-propanol solution 3 times, 2 minutes each time, treat with Clear Solution 1 for 15 minutes, Clear Solution 2 for 10 minutes, and then treat with PBST for 3 minutes.

[0059] The reagents used were prepared as follows:

[0060] Paraformaldehyde: Prepare a solution containing 4% paraformaldehyde and 0.8% sodium hydroxide with a pH of 7.2-7.4 in phosphate buffered saline (PBS). Refrigerate.

[0061] PBST: Prepare 0.04% Triton X-100 in PBS solution. Store at room temperature.

[0062] 60% 2-propanol solution: Prepare by diluting directly with fish embryo culture medium and prepare fresh before use.

[0063] Oil Red O solution: 0.3 g of Oil Red O was dissolved in 100 mL of 60% 2-propanol solution.

[0064] Bleach solution: Prepare a solution containing 3% H2O2 and 1% KOH in water. Prepare fresh before use.

[0065] Clearing Solution 1: Prepare a solution containing 20% ​​glycerol and 0.25% KOH in water. Store at room temperature.

[0066] Clearing Solution 2: Prepare a solution containing 50% glycerol and 0.25% KOH in water. Store at room temperature.

[0067] Place the zebrafish sideways. Then place it under a stereo microscope and take pictures with the same settings. Open the pictures with analysis software such as Image J, mark the intestinal area of ​​each zebrafish, then select "Measure Average Intensity" in the "Measurement" column, and select the "Average Signal Intensity" in the test results as the fat level.

[0068] Calculate the fat metabolism promotion rate:

[0069]

[0070] Where:

[0071] S—mean value of zebrafish fat level in solid beverage group;

[0072] M—mean value of zebrafish fat level in the model control group;

[0073] B—The average value of zebrafish fat level in the blank control group;

[0074] (2) Weight loss effect: zebrafish fat absorption inhibition test method

[0075] Healthy zebrafish embryos were selected and cultured at 28°C ± 1°C at a density of no more than 1 fish embryo in 200 μL fish embryo culture medium until 5 days after fertilization. 30 zebrafish were randomly selected for each treatment group and placed in a 6-well plate. The solid beverage group added 6 mL of fish embryo culture medium containing 1% egg yolk and 1.71 g / L solid beverage sample solution. The model control group added 6 mL of fish embryo culture medium containing 1% egg yolk solution. The blank control group added 6 mL of zebrafish embryo culture medium. The positive control group added 6 mL of fish embryo culture medium containing 1% egg yolk and 0.3 μM ezetimibe. All treatment groups were placed in a 28°C ± 1°C incubator for 48 h ± 1 h. At least 90% of the zebrafish survived after exposure in each test group, otherwise the results of the corresponding test group were invalid.

[0076] After fixing each test group of zebrafish in paraformaldehyde for at least 1 hour, treat the zebrafish with PBST for 2 minutes, repeat 3 times. Treat the zebrafish with bleach solution for 10 minutes. If treating in a test tube, keep the lid open. Treat with PBST for 2 minutes, then treat with 60% 2-propanol solution for 5 minutes. Stain with Oil Red O solution for 75 minutes ± 10 minutes. Immerse in 60% 2-propanol solution 3 times, 2 minutes each time, treat with Clear Solution 1 for 15 minutes, treat with Clear Solution 2 for 10 minutes, and then treat with PBST for 3 minutes. The preparation method of each solution is shown in "(1)".

[0077] Place the zebrafish sideways. Then place it under a stereo microscope and take pictures with the same settings. Open the pictures with analysis software such as Image J, mark the intestinal area of ​​each zebrafish, then select "Measure Average Intensity" in the "Measurement" column, and select the "Average Signal Intensity" in the test results as the fat level.

[0078] Calculate the fat absorption inhibition rate:

[0079]

[0080] Where:

[0081] S—mean value of zebrafish fat level in solid beverage group;

[0082] B—The average value of zebrafish fat level in the blank control group;

[0083] M—The average fat level of zebrafish in the model control group.

[0084] (3) Sugar Control Effect: Zebrafish Sugar Control Test Method

[0085] Healthy zebrafish embryos were selected and cultured at a density of no more than one fish embryo in 200 μL of fish embryo culture medium at 28°C ± 1°C until 6 days after fertilization.

[0086] 60 zebrafish were randomly selected and placed in a 6-well plate, with 20 zebrafish in each well. 5 mL of 4% rice solution (4% rice homogenate solution was prepared with fish embryo culture medium, freshly prepared before use) and solid beverage sample (final concentration was 1.71 g / L) were added to the solid beverage group. 5 mL of 4% rice solution was added to the model control group. 5 mL of zebrafish embryo culture medium was added to the blank control group. All treatment groups were placed in a 28°C ± 1°C incubator and cultured for 16 h ± 1 h. At least 90% of the zebrafish survived after exposure in each test group, otherwise the results of the corresponding test group were invalid.

[0087] The zebrafish from each test well were collected into a test tube, anesthetized in ice, washed once with fish embryo culture medium, homogenized, and then centrifuged at room temperature for 5 minutes and 15,000 rpm for 5 minutes. 1.5 μL of the supernatant was taken on a blood glucose meter test strip to measure the glucose level.

[0088] Calculate the inhibition rate of free sugar production:

[0089]

[0090] Where:

[0091] S—mean value of free sugar level in zebrafish in solid beverage group;

[0092] M—mean value of free sugar level in zebrafish of model control group;

[0093] B—The average free sugar level of zebrafish in the blank control group.

[0094] Table 2 Investigation of weight loss effects of solid beverages made with different formulas

[0095]

[0096] As can be seen from Table 2, the solid beverages described in the three embodiments of the present invention have significantly higher zebrafish fat metabolism promotion rate and fat absorption inhibition rate than not only the positive control group, but also significantly higher than treatment groups 4-6, indicating that the solid beverages described in the present invention can significantly improve the fat metabolism promotion rate and fat absorption inhibition rate through reasonable proportions, and have a significant weight loss effect.

[0097] The blood sugar suppression rates of the three example groups of the present invention were also improved to varying degrees compared with treatment groups 4 to 6, indicating that the solid beverage prepared by the present invention can effectively control blood sugar levels and significantly improve the blood sugar control effect.

[0098] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A hydrogel fiber composition having a weight loss effect, characterized in that: The invention comprises the following components in parts by weight: 15-25 parts of hydrogel cellulose powder, 1-5 parts of white bean extract, 1-5 parts of Dendrobium officinale extract, 1-3 parts of Polygonatum sibiricum extract, 0.5-1.5 parts of Buzha leaf extract, 0.3-0.7 parts of Wumei extract, 1-10 parts of structural fat, 1-10 parts of oligofructose, 5-15 parts of maltodextrin, 1-5 parts of Lactobacillus acidophilus powder, and 20 mg of sodium hyaluronate is added for every 2 g of the above components in total.

2. The composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 20 parts of hydrogel cellulose powder, 3 parts of white kidney bean extract, 3 parts of dendrobium officinale extract, 2 parts of polygonatum sibiricum extract, 1 part of buzia leaf extract, 0.5 parts of ebony extract, 5 parts of structural fat, 5 parts of oligofructose, 10 parts of maltodextrin and 3 parts of lactobacillus acidophilus powder.

3. The composition according to claim 1, characterized in that The preparation method of the Buzha leaf extract is: take Buzha leaves, add water to decoct, filter to obtain a filtrate, and dry the filtrate; the preparation method of the black plum extract is: take black plum, add water to decoct, filter to obtain a filtrate, and dry the filtrate; the amount of water added is 6-8 times the total mass of the Buzha leaves or black plums, the decoction time is 20-30 minutes, and the drying method is vacuum freeze drying.

4. The composition according to claim 1, characterized in that The preparation method of the described Lactobacillus acidophilus powder comprises the following steps: (1) Disinfection of the fermentation tank, followed by addition of culture medium and sterilization; (2) inoculating pure culture strains of Lactobacillus acidophilus into a fermentation tank medium for fermentation; (3) After fermentation, the fermentation liquid is spray-dried to obtain Lactobacillus acidophilus powder.

5. The method for preparing the composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: Take the formulated amounts of hydrogel cellulose powder, white kidney bean extract, dendrobium officinale extract, polygonatum sibiricum extract, buzia leaf extract, ebony extract, structural fat, oligofructose, maltodextrin and lactobacillus acidophilus powder and mix them evenly.

6. A hydrogel fiber solid beverage for weight loss, characterized in that: The invention comprises the composition according to any one of claims 1 to 5.

7. The method for preparing a solid beverage according to claim 6, characterized in that: The method comprises the following steps: taking the composition according to any one of claims 1 to 5, crushing and passing through a 20-60 mesh sieve to obtain a solid beverage.

8. Use of the composition according to any one of claims 1 to 5 or the solid beverage according to claim 6 in preparing a preparation for improving the fat metabolism promotion rate, and / or the fat absorption inhibition rate, and / or the blood sugar inhibition rate 9. Use of the composition according to any one of claims 1 to 5 or the solid beverage according to claim 6 in preparing a weight loss preparation.

10. The use according to claim 9, characterized in that The method of consumption is: take the composition or solid beverage, add warm water not exceeding 45°C and drink it, add 200 ml of warm water to every 2g of the composition; the composition or solid beverage is consumed 4-10 times a day, 2g each time.

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