An antioxidant composition containing bark extract of Tabebuia avellanedae and its application

Abstract

The present invention discloses an antioxidant composition containing the bark extract of Tabebuia aurea and its application, belonging to the technical field of cosmetics. The antioxidant composition containing the bark extract of Tabebuia aurea comprises the following components: the bark extract of Tabebuia aurea, acetyl hexapeptide-8, and squalane. And through the synergistic effect among these three components, the antioxidant composition has a good antioxidant effect, and the antioxidant composition obtained by the preparation method of the present invention is more easily absorbed by the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to an antioxidant composition containing the bark extract of Tabebuia impetiginosa and its application. Background Art

[0002] With the increase of age, problems such as skin aging, skin spots, and wrinkles become increasingly prominent, and free radicals play a key role in this process. Free radicals are triggered by environmental factors such as ultraviolet rays, pollution, and stress, leading to oxidative stress, damaging skin cells and tissues, and accelerating skin aging. To resist oxidative damage, antioxidants such as vitamin C, vitamin E, and green tea extract are often added to cosmetics. However, these traditional antioxidants have problems such as poor stability and low bioavailability, which limit the exertion of their efficacy. The introduction of nanotechnology brings new opportunities to antioxidant cosmetics. Carrier systems such as nanoemulsions can improve the solubility, stability, and bioavailability of active ingredients, enhancing the antioxidant effect. However, nanoemulsions require relatively high technical processes, are difficult to reduce costs, and existing antioxidant cosmetics mostly contain chemical additives, and the active substances are easily affected by the environment, with a short service life, making it difficult to meet the needs of consumers for natural, organic, and chemical-free products. Therefore, the development of natural antioxidant-based products has become a research hotspot and trend in the current cosmetics field. Summary of the Invention

[0003] The purpose of the present invention is to provide an antioxidant composition containing the bark extract of Tabebuia impetiginosa and its application. This antioxidant composition is formulated by compounding natural plant extracts, acetyl hexapeptide-8, and squalane, with high safety and significant antioxidant effects.

[0004] To achieve the above purpose, the present invention adopts the following technical solutions:

[0005] In the first aspect, the present invention provides an antioxidant composition containing the bark extract of Tabebuia impetiginosa, comprising the following components: the bark extract of Tabebuia impetiginosa, acetyl hexapeptide-8, and squalane.

[0006] Preferably, the antioxidant composition containing the bark extract of Tabebuia impetiginosa comprises the following components in parts by mass:

[0007] The bark extract of Tabebuia impetiginosa: 0.05 - 3 parts;

[0008] Acetyl hexapeptide-8: 0.01 - 5 parts;

[0009] Squalane: 0.01 - 1 part.

[0010] More preferably, the antioxidant composition containing the bark extract of Tabebuia impetiginosa comprises the following components in parts by mass:

[0011] The bark extract of Tabebuia impetiginosa: 2.5 - 3 parts;

[0012] Acetyl Hexapeptide-8: 1 - 5 parts;

[0013] Squalane: 0.2 - 1 part.

[0014] Most preferably, the antioxidant composition containing the bark extract of *Brunfelsia uniflora* contains the following components in parts by mass:

[0015] Bark extract of *Brunfelsia uniflora*: 2.5 parts;

[0016] Acetyl Hexapeptide-8: 1 part;

[0017] Squalane: 0.2 part.

[0018] In a second aspect, the present invention provides a method for preparing the bark extract of *Brunfelsia uniflora* described in the first aspect, and the preparation method includes the following steps:

[0019] S1. Air-dry the bark of *Brunfelsia uniflora* until the water content ≤ 5%, and grind it into coarse powder;

[0020] S2. Mix the coarse powder of the bark of *Brunfelsia uniflora* with rapeseed oil and polyglyceryl-3 diisostearate, heat to 50 - 60 °C, and stir at 300 rpm for 10 - 30 min to obtain a mixed solution;

[0021] S3. Under N₂ atmosphere, perform ultrasonic-assisted extraction on the mixed solution in S2 to obtain an extract, extraction temperature: 40 - 60 °C, ultrasonic frequency: 20 - 40 kHz, extraction time: 30 - 60 min;

[0022] S4. Filter the extract to remove insoluble substances, centrifuge, and take the supernatant to obtain the bark extract of *Brunfelsia uniflora*;

[0023] Among them, in step S2, the mass ratio of the coarse powder of the bark of *Brunfelsia uniflora*, rapeseed oil, and polyglyceryl-3 diisostearate is 1: 1.6 - 12: 0.04 - 1.2.

[0024] In a third aspect, the present invention provides a cream with antioxidant and anti-aging effects, and the cream with antioxidant and anti-aging effects is composed of the following components in mass percentage:

[0025] The antioxidant composition containing the bark extract of *Brunfelsia uniflora* described in the first aspect 1 - 3 wt%;

[0026] Humectant: 2 - 10 wt%;

[0027] Emollient: 5 - 20 wt%;

[0028] Emulsifier: 5 - 10 wt%;

[0029] Preservative: 0.2 - 0.5 wt%;

[0030] Thickener: 0.1 - 1 wt%;

[0031] Make up to 100 wt% with deionized water;

[0032] The moisturizer is one or more of glycerol, propylene glycol, and butylene glycol;

[0033] The emollient is one or more of petrolatum, shea butter, isocetane, and isopropyl isostearate;

[0034] The preservative is one or more of methylparaben, sodium benzoate, p - hydroxyacetophenone, and sorbic acid;

[0035] The emulsifier is one or more of cetearyl alcohol, PEG - 100 stearate, and glyceryl stearate.

[0036] The thickener is one or more of xanthan gum, hydroxyethyl cellulose, acryloyldimethyltaurate / VP copolymer, and sodium polyacryloyldimethyltaurate.

[0037] Fourthly, the present invention provides a preparation method of the cream described in the third aspect, and the specific steps are as follows:

[0038] S1: Mix the moisturizer, preservative, thickener with deionized water, heat to 60 - 80 °C and stir, stirring speed: 200 - 400 rpm, stirring time: 1 - 3 min; then homogenize, homogenization speed: 5000 - 7000 rpm, homogenization time: 1 - 3 min, to obtain mixture A;

[0039] S2: Mix the emollient and emulsifier, heat to 60 - 80 °C and stir, stirring speed: 200 - 400 rpm, stirring time: 1 - 3 min; then homogenize, homogenization speed: 5000 - 7000 rpm, homogenization time: 1 - 3 min, to obtain mixture B;

[0040] S3: Add mixture B to mixture A, stir, stirring speed: 200 - 400 rpm, stirring time: 3 - 5 min; then homogenize, homogenization speed: 5000 - 7000 rpm, homogenization time: 3 - 5 min, to obtain the mixture;

[0041] S4: When the liquid to be mixed cools down to 30 - 50 °C, mix the antioxidant composition with the liquid to be mixed and stir at a stirring speed of 200 - 400 rpm for 1 - 3 min; then homogenize at a homogenization speed of 5000 - 7000 rpm for 1 - 3 min, and continue to cool down to 20 - 25 °C to obtain a cream with antioxidant and anti-aging effects, fill it, sterilize it, seal it, and store it at room temperature.

[0042] The antioxidant composition containing the bark extract of Tabebuia impetiginosa in the present invention comprises the bark extract of Tabebuia impetiginosa, acetyl hexapeptide-8, and squalane. Among them, the bark extract of Tabebuia impetiginosa contains polyphenols (such as flavonoids, tannins), polyols, and terpene compounds, which can directly scavenge free radicals (ROS, hydroxyl radicals), inhibit lipid peroxidation reactions, and provide electrons to neutralize oxidized active molecules; acetyl hexapeptide-8 reduces oxidative stress caused by inflammation or stress by inhibiting the release of neurotransmitters (such as catecholamines), and can activate cell signaling pathways, promote collagen synthesis, and repair the oxidized extracellular matrix; squalane reduces transepidermal water loss by mimicking the sebum film structure and prevents the penetration of external oxidizing factors (such as ozone, etc.).

[0043] In the present invention, rapeseed oil containing polyglyceryl-3 diisostearate is used to extract the bark of Tabebuia impetiginosa. By utilizing the similar solubility characteristics of rapeseed oil, it can enrich lipophilic active substances such as terpenoids (such as triterpenoids, sesquiterpenoids), steroids (such as steroid saponins, steroid hormones), fatty acids (such as fatty acids, fatty oils, etc.), and volatile oils (such as volatile alcohols, alkenes, etc.) in the bark of Tabebuia impetiginosa. In addition, the addition of polyglyceryl-3 diisostearate can further improve the biological activity and enrichment rate of the bark extract of Tabebuia impetiginosa.

[0044] Compared with the prior art, the beneficial effects of the present invention are as follows: The combined use of the bark extract of Tabebuia impetiginosa, acetyl hexapeptide-8, and squalane in the present invention can achieve multi-level synergistic effects.

[0045] First, synergistic antioxidant defense: Squalane can form a physical barrier on the surface to reduce the invasion of oxidative stress sources and stabilize active ingredients at the same time; the bark extract of Tabebuia impetiginosa can quickly scavenge the generated free radicals and reduce the accumulation of oxidative damage; acetyl hexapeptide-8 can activate endogenous antioxidant enzymes and repair damaged cells, blocking the vicious cycle of oxidation.

[0046] Secondly, synergistic anti-inflammation and repair: The anti-inflammatory components in the bark extract of *Tabebuia aurea* can effectively reduce the release of inflammatory factors, while acetyl hexapeptide-8 inhibits neurogenic inflammation. The two together reduce inflammation-related oxidative stress. Meanwhile, squalane reduces the penetration of inflammatory factors through barrier repair, forming a positive "anti-inflammatory - antioxidant" cycle. Moreover, the lipophilic property of squalane enables squalane to encapsulate polar molecules (polyphenols) in the bark extract of *Tabebuia aurea*, improving its stability and transdermal absorption rate.

[0047] In summary, in the present invention, the bark extract of *Tabebuia aurea*, acetyl hexapeptide-8, and squalane achieve a synergistic antioxidant effect through a three-dimensional network of "physical barrier - free radical scavenging - endogenous repair", and the carrier function of squalane further amplifies the efficacy, forming an efficient and long-lasting antioxidant system. Detailed implementation manners

[0048] To better understand the present invention, the following further describes the present invention in combination with specific embodiments. The terms used in the embodiments are for describing specific implementation manners and do not constitute a limitation on the protection scope of the present invention.

[0049] For the experimental methods without specific conditions noted in the following tests, they are generally carried out under conventional conditions or according to the conditions recommended by the manufacturers. Unless otherwise stated, percentages and parts are calculated by weight.

[0050] Some raw materials and their sources are as follows:

[0051] Bark of *Tabebuia aurea*: Purchased from Shanghai Qianfei Chemical Co., Ltd.

[0052] Acetyl hexapeptide-8: Purchased from Shanghai Qianfei Chemical Co., Ltd.

[0053] Squalane: Purchased from Yichun Great Turtle Life Sciences Co., Ltd.

[0054] Rapeseed oil: Purchased from Jishui Hongxing Natural Medicinal Spice Oil Factory.

[0055] Polyglycerol-3 diisostearate: Purchased from Guangzhou Aoxue Chemical Co., Ltd.

[0056] Other raw materials or reagents are all commercially available as usual.

[0057] The preparation method of the bark extract of *Tabebuia aurea* described in the present invention is specifically as follows:

[0058] Bark extract of *Tabebuia aurea* 1:

[0059] S1. Air-dry the bark of *Tabebuia aurea* until the water content ≤ 5%, and grind it into coarse powder;

[0060] S2. Mix the coarse powder of the bark of *Brunfelsia latifolia* with rapeseed oil and polyglyceryl-3 diisostearate, heat to 55 °C, and stir at 300 rpm for 20 min to obtain a mixture.

[0061] S3. Under a N₂ atmosphere, perform ultrasonic-assisted extraction on the mixture in S2 to obtain an extract. Extraction temperature: 55 °C, ultrasonic frequency: 30 kHz, extraction time: 45 min.

[0062] S4. Filter the extract to remove insoluble substances, centrifuge, and take the supernatant to obtain the bark extract of *Brunfelsia latifolia*.

[0063] Among them, the mass ratio of the coarse powder of the bark of *Brunfelsia latifolia*, rapeseed oil, and polyglyceryl-3 diisostearate in step S2 is 1:3:0.2.

[0064] Bark extract of *Brunfelsia latifolia* 2:

[0065] S1. Air-dry the bark of *Brunfelsia latifolia* until the water content ≤ 5%, and grind it into a coarse powder.

[0066] S2. Mix the coarse powder of the bark of *Brunfelsia latifolia* with rapeseed oil and polyglyceryl-3 diisostearate, heat to 60 °C, and stir at 300 rpm for 10 min to obtain a mixture.

[0067] S3. Under a N₂ atmosphere, perform ultrasonic-assisted extraction on the mixture in S2 to obtain an extract. Extraction temperature: 60 °C, ultrasonic frequency: 20 kHz, extraction time: 60 min.

[0068] S4. Filter the extract to remove insoluble substances, centrifuge, and take the supernatant to obtain the bark extract of *Brunfelsia latifolia*.

[0069] Among them, the mass ratio of the coarse powder of the bark of *Brunfelsia latifolia*, rapeseed oil, and polyglyceryl-3 diisostearate in step S2 is 1:1.6:1.2.

[0070] Bark extract of *Brunfelsia latifolia* 3:

[0071] S1. Air-dry the bark of *Brunfelsia latifolia* until the water content ≤ 5%, and grind it into a coarse powder.

[0072] S2. Mix the coarse powder of the bark of *Brunfelsia latifolia* with rapeseed oil and polyglyceryl-3 diisostearate, heat to 50 °C, and stir at 300 rpm for 30 min to obtain a mixture.

[0073] S3. Under a N₂ atmosphere, perform ultrasonic-assisted extraction on the mixture in S2 to obtain an extract. Extraction temperature: 40 °C, ultrasonic frequency: 40 kHz, extraction time: 30 min.

[0074] S4. Filter the extract to remove insoluble substances, centrifuge it, and take the supernatant to obtain the bark extract of *Tabebuia pallida*:

[0075] Among them, in step S2, the mass ratio of the crude powder of the bark of *Tabebuia pallida*, rapeseed oil, and polyglyceryl-3 diisostearate is 1:12:0.04.

[0076] Bark extract of *Tabebuia pallida* ①:

[0077] Different from the bark extract of *Tabebuia pallida* 1, an equal amount of water is used to replace rapeseed oil, and other steps and parameters are the same as those of the bark extract of *Tabebuia pallida* 1.

[0078] Bark extract of *Tabebuia pallida* ②:

[0079] Different from the bark extract of *Tabebuia pallida* 1, an equal amount of 60 wt% ethanol solution is used to replace rapeseed oil, and other steps and parameters are the same as those of the bark extract of *Tabebuia pallida* 1.

[0080] Bark extract of *Tabebuia pallida* ③:

[0081] Different from the bark extract of *Tabebuia pallida* 1, polyglyceryl-3 diisostearate is not added in step S2, and other steps and parameters are the same as those of the bark extract of *Tabebuia pallida* 1.

[0082] Bark extract of *Tabebuia pallida* ④:

[0083] Different from the bark extract of *Tabebuia pallida* 1, in step S2, the mass ratio of the crude powder of the bark of *Tabebuia pallida*, rapeseed oil, and polyglyceryl-3 diisostearate is 1:0.2:3.

[0084] The specific mass parts of each component in the antioxidant composition containing the bark extract of *Tabebuia pallida* according to the present invention are as follows:

[0085] Antioxidant composition 1:

[0086] Bark extract of *Tabebuia pallida* (bark extract of *Tabebuia pallida* 1): 2.5 parts; acetyl hexapeptide-8: 1 part; squalane: 0.2 part.

[0087] Antioxidant composition 2:

[0088] Bark extract of *Tabebuia pallida* (bark extract of *Tabebuia pallida* 1): 3 parts; acetyl hexapeptide-8: 0.01 part; squalane: 1 part.

[0089] Antioxidant composition 3:

[0090] Bark extract of *Tabebuia pallida* (bark extract of *Tabebuia pallida* 1): 0.05 part; acetyl hexapeptide-8: 5 parts; squalane: 0.01 part.

[0091] Antioxidant composition ①:

[0092] Different from Composition 1, it lacks the bark extract of Tabebuia avellanedae, and the lacking parts are supplemented by acetyl hexapeptide-8 and squalane with a mass ratio of 1:0.2.

[0093] Antioxidant composition ②:

[0094] Different from Composition 1, it lacks acetyl hexapeptide-8, and the lacking parts are supplemented by the bark extract of Tabebuia avellanedae and squalane with a mass ratio of 2.5:0.2.

[0095] Antioxidant composition ③:

[0096] Different from Composition 1, it lacks squalane, and the lacking parts are supplemented by the bark extract of Tabebuia avellanedae and acetyl hexapeptide-8 with a mass ratio of 2.5:1.

[0097] Antioxidant composition ④:

[0098] Bark extract of Tabebuia avellanedae ①: 2.5 parts; Acetyl hexapeptide-8: 1 part; Squalane: 0.2 part.

[0099] Antioxidant composition ⑤:

[0100] Bark extract of Tabebuia avellanedae ②: 2.5 parts; Acetyl hexapeptide-8: 1 part; Squalane: 0.2 part.

[0101] Antioxidant composition ⑥:

[0102] Bark extract of Tabebuia avellanedae ③: 2.5 parts; Acetyl hexapeptide-8: 1 part; Squalane: 0.2 part.

[0103] Antioxidant composition ⑦:

[0104] Bark extract of Tabebuia avellanedae ④: 2.5 parts; Acetyl hexapeptide-8: 1 part; Squalane: 0.2 part.

[0105] A cream with antioxidant and anti-aging effects, and the mass percentages of its components are shown in Table 1 below.

[0106] Table 1 Components and their mass percentages of the cream with antioxidant and anti-aging effects

[0107]

[0108] Note: The moisturizer is glycerin; the emulsifier is cetearyl alcohol, glyceryl stearate, and PEG-100 stearate, and the mass ratio of cetearyl alcohol, glyceryl stearate, and PEG-100 stearate is 1:1:1; the emollient is shea butter; the preservative is p-hydroxyacetophenone; the thickener is xanthan gum and sodium polyacryloyldimethyltaurate, and the mass ratio of xanthan gum and sodium polyacryloyldimethyltaurate is 2:3.

[0109] The specific preparation steps of the above-mentioned creams 1-5 and creams ①-⑦ are as follows:

[0110] S1: Mix the moisturizer, preservative, thickener with deionized water, heat to 70°C and stir, stirring speed: 300 rpm, stirring time: 2 min; then homogenize, homogenization speed: 6000 rpm, homogenization time: 2 min, to obtain mixture A;

[0111] S2: Mix the emollient and emulsifier, heat to 70°C and stir, stirring speed: 300 rpm, stirring time: 2 min; then homogenize, homogenization speed: 6000 rpm, homogenization time: 2 min, to obtain mixture B;

[0112] S3: Add mixture B to mixture A, stir, stirring speed: 300 rpm, stirring time: 5 min; then homogenize, homogenization speed: 6000 rpm, homogenization time: 5 min, to obtain a mixture;

[0113] S4: Wait for the mixture to cool to 40°C, mix the antioxidant composition with the mixture, stir, stirring speed: 300 rpm, stirring time: 2 min; then homogenize, homogenization speed: 6000 rpm, homogenization time: 2 min, continue to cool to 25°C, to obtain a cream with antioxidant and anti-aging effects, fill, sterilize, seal, and store at room temperature.

[0114] The preparation method of the blank cream is as follows:

[0115] S1: Mix the moisturizer, preservative, thickener with deionized water, heat to 70°C and stir, stirring speed: 300 rpm, stirring time: 2 min; then homogenize, homogenization speed: 6000 rpm, homogenization time: 2 min, to obtain mixture A;

[0116] S2: Mix the emollient and emulsifier, heat to 70°C and stir, stirring speed: 300 rpm, stirring time: 2 min; then homogenize, homogenization speed: 6000 rpm, homogenization time: 2 min, to obtain mixture B;

[0117] S3: Add mixture B to mixture A, stir at a stirring speed of 300 rpm for 5 min; then homogenize at a homogenization speed of 6000 rpm for 5 min to obtain a mixture.

[0118] S4: Wait for the mixed liquid to continue cooling to room temperature of 40°C, stir at a stirring speed of 300 rpm for 2 min; then homogenize at a homogenization speed of 6000 rpm for 2 min, and continue cooling to 25°C to obtain a blank cream, which is filled, sterilized, sealed, and stored at room temperature.

[0119] Experiment 1: Human safety test

[0120] 1-1. Human skin patch test

[0121] Test substance: Cream 1-5.

[0122] Subject selection: Select healthy volunteers aged 21-57 years old, who are willing to participate and sign an informed consent form, can complete the experiment as required during the test period, are randomly grouped, and 30 subjects are set in each group.

[0123] Experimental method: Select qualified patch equipment, use the closed patch test method, take 1 g of the test substance and apply it evenly in the patch tester, then use an external low-sensitization tape to apply it to the flexor side of the subject's forearm. After 24 hours, remove the test substance, and observe and record the skin reaction at 0.5, 24, and 48 hours after removal respectively.

[0124] Result evaluation: The skin adverse reaction grading is judged according to the grading of skin adverse reactions in the "Technical Specifications for Cosmetics Safety" (2015 version). The grading standard is shown in Table 2, and the results are shown in Table 3.

[0125] Table 2 Skin adverse reaction grading standard

[0126] Level Classification Phenomenon Level 0 No skin reaction Level 1 Appearance of faint erythema on the skin Level 2 Appearance of erythema, infiltration and papules on the skin Level 3 Appearance of erythema, edema, papules and blisters on the skin Level 4 Appearance of erythema, edema and large blisters on the skin

[0127] Table 3 Results of human skin patch test

[0128]

[0129] 1-2. Human test on sensitive skin

[0130] Test substance: Cream 1-5 and Cream ①-⑦.

[0131] Subject selection: Select healthy volunteers aged 21-44 years old, who meet the positive result of the lactic acid stinging test, are willing to participate and sign an informed consent form, can complete the experiment as required during the test period, are randomly grouped, and 30 subjects are set in each group.

[0132] Experimental method: Volunteers randomly divided their bilateral faces into a test side and a control side. After cleansing the skin, the product was evenly applied to the facial skin on the test side according to the product usage method, and no product was used on the control side. After 28 days of trial use, the volunteers reported the local skin reactions. If there were any adverse reactions, a dermatologist would evaluate the situation of the adverse reactions.

[0133] Result evaluation: The grading of skin adverse reactions was judged according to the grading of skin adverse reactions in the "Technical Specifications for Cosmetics Safety" (2015 edition). The grading criteria are shown in Table 1, and the results are shown in Table 4.

[0134] Table 4 Results of human trial

[0135]

[0136] From the results of the human skin patch test, during the test period, the safety of Creams 1-5 was good, and there were no adverse reactions in the subjects.

[0137] From the results of the test for sensitive skin suitability, during the test period, no subjects had skin adverse reactions to Creams 1-5 and Creams ①-⑦, indicating that the safety of Creams 1-5 and Creams ①-⑦ was good, mild and non-irritating, and suitable for people with sensitive skin.

[0138] Experiment 2: Determination of antioxidant test

[0139] Sample solution: The sample solution was a mixed solution prepared by dissolving Creams 1-5, Creams ①-⑦ and blank cream in absolute ethanol at a concentration of 5 mg / mL.

[0140] Test steps:

[0141] 1. Take 2 mL of the sample solution and mix it with 2 mL of 2×10 -4 moL / L DPPH absolute ethanol solution (A1). DPPH (1,1-diphenyl-2-picrylhydrazyl) is a commonly used reagent for testing free radical scavenging ability;

[0142] 2. Take 2 mL of absolute ethanol and mix it with 2 mL of 2×10 -4 moL / L DPPH absolute ethanol solution (A2);

[0143] 3. Take 2 mL of absolute ethanol and mix it with 2 mL of the sample solution (A3);

[0144] 4. After A1, A2, and A3 have reacted for 40 minutes, use an ultraviolet spectrophotometer to measure the absorbance values of A1, A2, and A3 at 517 nm.

[0145] Clearance rate calculation formula: Clearance rate (%) = [1 - (A1 - A3) / A2] × 100%

[0146] The experimental results are shown in Table 5.

[0147] Table 5 Antioxidant effect

[0148] Sample Name Free radical DPPH scavenging rate (%) Facial Cream 1 <![CDATA[94.59±0.34 * <!-- 9 -->]]> Facial Cream 2 <![CDATA[93.67±0.23 * > Facial Cream 3 <![CDATA[94.74±0.14 * > Facial Cream 4 <![CDATA[90.73±0.63 * > Facial Cream 5 <![CDATA[92.14±0.96 * > Facial Cream ① <![CDATA[43.62±1.10 *a > Facial Cream ② <![CDATA[63.40±0.38 *a > Facial Cream ③ <![CDATA[81.25±0.44 *a > Facial Cream ④ <![CDATA[68.33±0.71 *a > Facial Cream ⑤ <![CDATA[74.81±1.37 *a > Facial Cream ⑥ <![CDATA[87.39±0.15 *a > Facial Cream ⑦ <![CDATA[82.74±0.80 *a > Blank Facial Cream 18.52±0.27

[0149] Note: "*" indicates P < 0.05 compared with the blank cream; "a" indicates p < 0.05 compared with Cream 1.

[0150] Comparing Creams 1, 4, and 5, the results show that Cream 1 has the best antioxidant effect. Therefore, Cream 1 is selected as the best example.

[0151] Comparing Cream 1 with Creams ①-⑦, the results show that there is a significant synergistic effect among the three components of the bark extract of Tabebuia impetiginosa, acetyl hexapeptide-8, and squalane provided by the present invention. Specifically: comparing Cream 1 with Creams ①-③, it can be seen that there is a certain synergistic effect among the bark extract of Tabebuia impetiginosa, acetyl hexapeptide-8, and squalane in the present invention; comparing Cream 1 with Creams ④-⑥, it can be seen that the preparation process of the bark extract of Tabebuia impetiginosa in the present invention has a certain influence on the antioxidant efficacy of the bark extract of Tabebuia impetiginosa and the cream containing it. Among them, the selection of the extraction solvent will affect the antioxidant efficacy of the bark extract of Tabebuia impetiginosa and the cream containing it, and adding polyglyceryl-3 diisostearate to rapeseed oil can more effectively dissolve the active substances in the bark of Tabebuia impetiginosa, thereby enhancing the antioxidant efficacy of the bark extract of Tabebuia impetiginosa; comparing Cream 1 with Cream ⑦, it can be seen that polyglyceryl-3 diisostearate and rapeseed oil have a significant synergistic effect in the mass ratio defined in the present invention.

[0152] The embodiments described above are some, but not all, of the embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application claimed, but merely represents the selected embodiments of the present application. All other embodiments obtained by those skilled in the art based on the embodiments in the present application without creative efforts fall within the scope of protection of the present application.

Claims

1. An antioxidant composition containing the bark extract of Tabebuia impetiginosa, characterized in that, The antioxidant composition consists of the following components in parts by mass: Bark extract of Tabebuia avellanedae: 0.05 - 3 parts; Acetyl hexapeptide - 8: 0.01 - 5 parts; Squalane: 0.01 - 1 part; The preparation steps of the bark extract of Tabebuia avellanedae are as follows: S1. Air - dry the bark of Tabebuia avellanedae until the water content ≤ 5%, and grind it into coarse powder; S2. Mix the coarse powder of the bark of Tabebuia avellanedae with rapeseed oil and polyglyceryl - 3 diisostearate, heat to 50 - 60 °C, and stir at 300 rpm for 10 - 30 min to obtain a mixed solution; S3. Under N2 atmosphere, perform ultrasonic - assisted extraction on the mixed solution in S2 to obtain an extract. Extraction temperature: 40 - 60 °C, ultrasonic frequency: 20 - 40 kHz, extraction time: 30 - 60 min; S4. Filter the extract to remove insoluble substances, centrifuge, and take the supernatant to obtain the bark extract of Tabebuia avellanedae; Among them, in step S2, the mass ratio of the coarse powder of the bark of Tabebuia avellanedae, rapeseed oil, and polyglyceryl - 3 diisostearate is 1:1.6 - 12:0.04 - 1.

2.

2. The antioxidant composition according to claim 1, wherein The antioxidant composition consists of the following components in parts by mass: Bark extract of Tabebuia avellanedae: 2.5 - 3 parts; Acetyl hexapeptide - 8: 1 - 5 parts; Squalane: 0.2 - 1 part.

3. Use of an antioxidant composition as described in claim 1 or 2 in the preparation of a cosmetic having antioxidant and anti - aging effects.

4. The application according to claim 3, wherein The dosage form of the cosmetic having antioxidant and anti - aging effects is any one of cream, lotion, and essence.

5. A cream with antioxidant and anti-aging effects, characterized in that, The cream contains 1 - 3 wt% of the antioxidant composition as described in claim 1 or 2.

6. The facial cream with antioxidant and anti-aging effects according to claim 5, characterized in that, The cream also contains the following components in mass percentage: Humectant: 2 - 10 wt%; Emollient: 5 - 20 wt%; Emulsifier: 5 - 10 wt%; Preservative: 0.2 - 0.5 wt%; Thickener: 0.1 - 1 wt%; Deionized water is added to 100 wt%.

7. The facial cream according to claim 6, wherein The humectant is one or more of glycerol, propylene glycol, and butylene glycol; the preservative is one or more of methylparaben, sodium benzoate, p - hydroxyacetophenone, and sorbic acid.

8. The face cream according to claim 6, characterized in that The emulsifier is one or more of cetearyl alcohol, PEG - 100 stearate, and glyceryl stearate.

9. The facial cream according to claim 6, wherein The emollient is one or more of petrolatum, shea butter, isocetane, and isopropyl isostearate; the thickener is one or more of xanthan gum, hydroxyethyl cellulose, acryloyldimethyltauramide / VP copolymer, and sodium polyacryloyldimethyltaurate.

10. A method for preparing a facial cream according to any one of claims 6-9, characterized in that, Comprising the following steps: S1: Mix the humectant, preservative, thickener with deionized water, heat to 60 - 80 °C and stir, stirring speed: 200 - 400 rpm, stirring time: 1 - 3 min; then homogenize, homogenization speed: 5000 - 7000 rpm, homogenization time: 1 - 3 min to obtain a mixed solution A; S2: Mix the emollient with the emulsifier, heat to 60 - 80 °C and stir at a stirring speed of 200 - 400 rpm for a stirring time of 1 - 3 min; then homogenize at a homogenization speed of 5000 - 7000 rpm for a homogenization time of 1 - 3 min to obtain mixture B. S3: Add mixture B to mixture A and stir at a stirring speed of 200 - 400 rpm for a stirring time of 3 - 5 min; then homogenize at a homogenization speed of 5000 - 7000 rpm for a homogenization time of 3 - 5 min to obtain a mixture. S4: Wait for the mixture to cool to 30 - 50 °C, mix the antioxidant composition with the mixture and stir at a stirring speed of 200 - 400 rpm for a stirring time of 1 - 3 min; then homogenize at a homogenization speed of 5000 - 7000 rpm for a homogenization time of 1 - 3 min, continue to cool to 20 - 25 °C to obtain a cream with antioxidant and anti-aging effects, fill, sterilize, seal, and store at room temperature.

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