Mussel anti-aging essence and preparation method thereof

Abstract

The invention relates to mussel anti-aging essence and a preparation method thereof, and belongs to the technical field of cosmetics. According to the invention, supercritical carbon dioxide is used for extracting effective components in mussels, fat-soluble active substances are efficiently extracted while degradation of thermosensitive components is reduced, and the lysine polypeptide liquid, the curry silk peptide and the youthful yeast are combined to provide the functions of enhancing skin elasticity and promoting cell regeneration; by adding the reverse-age cell chip, aging is delayed by simulating a cell environment or providing cell energy, and the skin is protected from oxidative stress by using antioxidant components, namely ergothioneine and pine mushroom extract.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and relates to a mussel anti-aging essence and a preparation method thereof. Background Art

[0002] Cell senescence refers to the process in which cells gradually decline in their proliferation and differentiation abilities and physiological functions over time as they perform their life activities. The life course of cells goes through several stages: undifferentiation, differentiation, growth, maturation, aging, and death. Aged and dead cells are cleared by the body's immune system, while new cells are constantly generated from the corresponding tissues and organs to make up for the aged and dead cells. The dynamic balance between cell aging and death and the growth of new cells is the basis for maintaining the normal life activities of the body.

[0003] Characteristics of cell senescence: First, cell cycle arrest: This is the most basic and most significant feature of cell senescence, which is manifested as the loss of cell proliferation ability and the inability to enter mitosis in response to normal stimulation. This stagnation is usually irreversible unless its proliferation ability is restored through certain interventions. Second, secretory changes: This refers to the production and release of a series of factors with pro-inflammatory, immunomodulatory, matrix remodeling and other effects by senescent cells, collectively referred to as the senescence-associated secretory phenotype (SASP). SASP can affect normal or abnormal cells around it and participate in the establishment and regulation of tissue microenvironment. Third, metabolic disorders: This refers to abnormal changes in energy metabolism, redox balance, autophagy levels, etc. in senescent cells, which causes them to produce more reactive oxygen and waste substances, and reduces their ability to resist oxidative stress and remove damaged substances. Fourth, macromolecular damage: This refers to irreparable or difficult-to-repair damage to senescent cells at the level of macromolecules such as DNA, proteins, and lipids, resulting in their functional failure or abnormality.

[0004] However, currently there are few anti-aging products on the cosmetics market in my country that are developed based on the mechanism at the cellular level. In addition, due to the single mechanism, the anti-aging effect applied to the products is poor, so its related performance still needs to be improved. Therefore, the present invention relates to a method for preparing mussel anti-aging essence. Summary of the invention

[0005] The present invention relates to a mussel anti-aging essence and a preparation method thereof, and belongs to the technical field of cosmetics. The present invention uses supercritical carbon dioxide to extract effective ingredients in mussels, efficiently extracts fat-soluble active substances while reducing the degradation of heat-sensitive components, combines lysine polypeptide liquid, kellis peptide, and youth yeast to provide functions of enhancing skin elasticity and promoting cell regeneration, adds anti-aging cell chips to delay aging by simulating cell environment or providing cell energy, and protects the skin from oxidative stress with antioxidant ingredients ergothioneine and matsutake extract.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A mussel anti-aging essence comprises the following raw materials in parts by weight: 1-32 parts of polypeptide liquid, 0.2-5 parts of anti-aging cell chips, 0.5-2 parts of compound ergothioneine, 0.1-7 parts of ectoine, 1-3 parts of colis peptide, 1-3 parts of youth yeast, 0.2-10 parts of isosorbide anhydride dimethyl ether, 0.1-1 parts of life element, 0.01-1 parts of p-hydroxyacetophenone, 0.01-1 parts of 1,2-pentanediol, 0.01-1 parts of 1,2-hexanediol, 0.001-0.2 parts of mussel extract, 0.01-20 parts of xanthan gum and 50-97 parts of deionized water.

[0008] As a preferred technical solution of the present invention, the polypeptide liquid is arginine / lysine polypeptide and deionized water; the mass ratio of the arginine / lysine polypeptide and deionized water is 0.002-0.003:99.997-99.998.

[0009] As a preferred technical solution of the present invention, the anti-aging cell chip includes sodium mannose phosphate, mannose, glycerol and deionized water; the mass ratio of sodium mannose phosphate, mannose, glycerol and deionized water is 3-4:1-2:40-60:40-50.

[0010] As a preferred technical solution of the present invention, the composite ergothioneine includes ergothioneine, Agaricus pine extract, 1,2-pentanediol, and ethylhexylglycerol; the mass ratio of the ergothioneine, Agaricus pine extract, 1,2-pentanediol, and ethylhexylglycerol is 0.01-0.03:95-96:3-5:0.05-0.1.

[0011] As a preferred technical solution of the present invention, the colis peptide includes hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin; the mass ratio of hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin is 0.1-0.3:20-40:60-80:0.3-0.5:0.05-0.1.

[0012] As a preferred technical solution of the present invention, the youth yeast includes bifid yeast fermentation product lysate, glycerol, and 1,2-pentanediol; the mass ratio of the bifid yeast fermentation product lysate, glycerol, and 1,2-pentanediol is 70-80:15-25:3-8.

[0013] As a preferred technical solution of the present invention, the life element includes cornflower flower extract and methylpropanediol; the mass ratio of the cornflower flower extract and methylpropanediol is 30-50:50-70.

[0014] As a preferred technical solution of the present invention, the preparation method of the mussel extract is: fresh mussels are washed, freeze-dried and ground into powder, supercritical fluid extraction equipment is used with carbon dioxide as a supercritical fluid solvent, carbon dioxide is pressurized to 75-80 bar at 30±2°C to a supercritical state, mussel powder is placed in an extraction kettle, supercritical carbon dioxide is introduced to dissolve the fat-soluble components in the mussels, and mussel extract is obtained by pressure reduction, separation, concentration and purification.

[0015] Furthermore, the method for preparing the mussel anti-aging essence comprises the following steps:

[0016] (1) adding deionized water and xanthan gum to a stirring container and stirring evenly, then sequentially adding compound ergothioneine, tetrahydromethylpyrimidine carboxylic acid, life element, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol and mussel extract, and uniformly dispersing to form an aqueous phase;

[0017] (2) Add lysine polypeptide liquid, kellis peptide and youth yeast into another stirring container and mix evenly to form a polypeptide yeast mixture;

[0018] (3) adding the polypeptide yeast mixture to the aqueous phase and mixing evenly;

[0019] (4) adding the anti-aging cell chip and dimethyl isosorbide to mix evenly, adjusting the pH to 5.0-6.5 with citric acid, and homogenizing the essence with a high shear homogenizer to prepare a mussel anti-aging essence.

[0020] Beneficial effects of the present invention:

[0021] (1) The present invention extracts mussels through supercritical carbon dioxide. Supercritical carbon dioxide exhibits dual properties of being both liquid and gas. It has a solubility similar to that of liquid, can dissolve fat-soluble substances and has good diffusivity, and also has gas properties that can quickly penetrate into the tiny pores of solid raw materials. In mussel extraction, supercritical carbon dioxide can efficiently penetrate mussel powder and react with fat-soluble effective ingredients (such as bioactive lipids, antioxidants, etc.) therein to achieve efficient separation and extraction. Supercritical carbon dioxide extraction avoids the degradation of active ingredients during the high-temperature extraction process, and no organic solvent is required during the extraction process, leaving no solvent residue in the product.

[0022] (2) The present invention aims to enhance skin elasticity, promote cell regeneration, and protect the skin from oxidative stress by combining multiple active ingredients. The polypeptide chains in the lysine polypeptide liquid can penetrate deeply into the surface layer of the skin and promote the synthesis of collagen, thereby enhancing the elasticity and firmness of the skin. Corliss peptides regulate the intracellular signal transduction pathway, stimulate cell metabolism and proliferation, effectively reduce fine lines and wrinkles, and improve skin elasticity. Youth yeast is rich in multiple nutrients, enhances skin barrier function, promotes cell repair, and provides antioxidant protection. In addition, the antioxidant ingredients ergothioneine and pine mushroom extract work together to provide lasting antioxidant capacity. Ergothioneine can neutralize free radicals and prevent cell oxidative damage, while pine mushroom extract further enhances the antioxidant effect and helps maintain the health and vitality of the skin. Through the synergistic effect of these ingredients, the skin's anti-aging ability is effectively improved, making the skin smoother, firmer and younger.

[0023] (3) The anti-aging cell chip fundamentally delays cell aging by simulating a healthy cell environment and providing an energy source. The core ingredients include sodium mannose phosphate, mannose, glycerol and deionized water. Sodium mannose phosphate participates in the cell metabolic pathway, optimizes glycolysis and phosphorylation processes, increases ATP production, and provides sufficient energy for cells; mannose repairs the extracellular matrix and improves cell signaling. It also has antioxidant and anti-inflammatory functions, reducing the damage to cells by free radicals and inflammation. Glycerol protects the cell structure by enhancing cell hydration and maintaining cell membrane stability; deionized water provides a pure solvent environment and optimizes osmotic pressure balance. By providing energy, optimizing metabolism, anti-oxidation and maintaining hydration, the anti-aging cell chip not only delays cell aging, but also repairs the microenvironment, promotes cell health, vitality and regeneration. It is an efficient anti-aging technology solution. DETAILED DESCRIPTION

[0024] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0025] The Tricholoma matsutake extracts described in the following examples and comparative examples were purchased from Bloomage Biotechnology Co., Ltd.; the cornflower flower extracts were purchased from Bloomage Biotechnology Co., Ltd.

[0026] Example 1

[0027] A mussel anti-aging essence comprises the following raw materials in parts by weight: 16 parts of lysine polypeptide liquid, 2.6 parts of anti-aging cell chips, 1.7 parts of compound ergothioneine, 3.5 parts of ectoine, 2 parts of colis peptide, 2 parts of youth yeast, 5 parts of isosorbide anhydride dimethyl ether, 0.5 parts of life element, 0.05 parts of p-hydroxyacetophenone, 0.05 parts of 1,2-pentanediol, 0.05 parts of 1,2-hexanediol, 0.1 parts of mussel extract, 5 parts of xanthan gum, and 80 parts of deionized water.

[0028] The polypeptide liquid is arginine / lysine polypeptide and deionized water; the mass ratio of the arginine / lysine polypeptide and deionized water is 0.0024:99.9976.

[0029] The anti-aging cell chip includes sodium mannose phosphate, mannose, glycerol and deionized water; the mass ratio of sodium mannose phosphate, mannose, glycerol and deionized water is 3.4:15:50:45.1.

[0030] The composite ergothioneine comprises ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol; the mass ratio of the ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol is 0.02:95.895:4:0.085.

[0031] The colis peptide includes hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin; the mass ratio of the hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin is 0.2:30:69.3:0.425:0.075.

[0032] The young yeast comprises a bifid yeast fermentation product lysate, glycerol and 1,2-pentanediol; the mass ratio of the bifid yeast fermentation product lysate, glycerol and 1,2-pentanediol is 70:20:5.

[0033] The life element comprises cornflower flower extract and methylpropanediol; the mass ratio of the cornflower flower extract and methylpropanediol is 40:60.

[0034] The preparation method of the mussel extract comprises the following steps: washing fresh mussels, freeze-drying them and grinding them into powder; using supercritical fluid extraction equipment and carbon dioxide as a supercritical fluid solvent, pressurizing the carbon dioxide at 31° C. to 75 bar to a supercritical state; placing the mussel powder in an extraction kettle, introducing supercritical carbon dioxide to dissolve the fat-soluble components in the mussels, and obtaining the mussel extract by pressure reduction, separation, concentration and purification.

[0035] The method for preparing the mussel anti-aging essence comprises the following steps:

[0036] (1) adding deionized water and xanthan gum to a stirring container and stirring evenly, then sequentially adding compound ergothioneine, tetrahydromethylpyrimidine carboxylic acid, life element, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol and mussel extract, and uniformly dispersing to form an aqueous phase;

[0037] (2) Add lysine polypeptide liquid, kellis peptide and youth yeast into another stirring container and mix evenly to form a polypeptide yeast mixture;

[0038] (3) adding the polypeptide yeast mixture to the aqueous phase and mixing evenly;

[0039] (4) adding the anti-aging cell chip and dimethyl isosorbide to mix evenly, adjusting the pH to 6.0 with citric acid, and homogenizing the essence with a high shear homogenizer to prepare a mussel anti-aging essence.

[0040] Example 2

[0041] A mussel anti-aging essence comprises the following raw materials in parts by weight: 10 parts of lysine polypeptide liquid, 0.2 parts of anti-aging cell chip, 0.5 parts of compound ergothioneine, 2 parts of ectoine, 1 part of colis peptide, 1 part of youth yeast, 2 parts of isosorbide anhydride dimethyl ether, 0.1 parts of life element, 0.01 parts of p-hydroxyacetophenone, 0.01 parts of 1,2-pentanediol, 0.01 parts of 1,2-hexanediol, 0.005 parts of mussel extract, 1 part of xanthan gum, and 50 parts of deionized water.

[0042] The polypeptide liquid is arginine / lysine polypeptide and deionized water; the mass ratio of the arginine / lysine polypeptide to deionized water is 0.002:99.998.

[0043] The anti-aging cell chip includes sodium mannose phosphate, mannose, glycerol and deionized water; the mass ratio of sodium mannose phosphate, mannose, glycerol and deionized water is 3:1:40:40.

[0044] The composite ergothioneine comprises ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol; the mass ratio of the ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol is 0.01:95:3:0.05.

[0045] The colis peptide includes hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin; the mass ratio of the hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin is 0.1:20:60:0.3:0.05.

[0046] The young yeast comprises a bifid yeast fermentation product lysate, glycerol and 1,2-pentanediol; the mass ratio of the bifid yeast fermentation product lysate, glycerol and 1,2-pentanediol is 70:15:3.

[0047] The life element comprises cornflower flower extract and methylpropanediol; the mass ratio of the cornflower flower extract and methylpropanediol is 33:50.

[0048] The preparation method of the mussel extract comprises the following steps: washing fresh mussels, freeze-drying them and grinding them into powder; using supercritical fluid extraction equipment and carbon dioxide as a supercritical fluid solvent, pressurizing the carbon dioxide at 75 bar at 30° C. to a supercritical state; placing the mussel powder in an extraction kettle, introducing supercritical carbon dioxide to dissolve the fat-soluble components in the mussels, and obtaining the mussel extract by pressure reduction, separation, concentration and purification.

[0049] The method for preparing the mussel anti-aging essence comprises the following steps:

[0050] (1) adding deionized water and xanthan gum to a stirring container and stirring evenly, then sequentially adding compound ergothioneine, tetrahydromethylpyrimidine carboxylic acid, life element, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol and mussel extract, and uniformly dispersing to form an aqueous phase;

[0051] (2) Add lysine polypeptide liquid, kellis peptide and youth yeast into another stirring container and mix evenly to form a polypeptide yeast mixture;

[0052] (3) adding the polypeptide yeast mixture to the aqueous phase and mixing evenly;

[0053] (4) adding the anti-aging cell chip and dimethyl isosorbide to mix evenly, adjusting the pH to 5.0 with citric acid, and homogenizing the essence with a high shear homogenizer to prepare a mussel anti-aging essence.

[0054] Example 3

[0055] A mussel anti-aging essence comprises the following raw materials in parts by weight: 32 parts of lysine polypeptide liquid, 5 parts of anti-aging cell chips, 2 parts of compound ergothioneine, 7 parts of ectoine, 3 parts of colis peptide, 3 parts of youth yeast, 10 parts of isosorbide anhydride dimethyl ether, 1 part of life element, 1 part of p-hydroxyacetophenone, 1 part of 1,2-pentanediol, 1 part of 1,2-hexanediol, 0.2 parts of mussel extract, 20 parts of xanthan gum, and 97 parts of deionized water.

[0056] The polypeptide liquid is arginine / lysine polypeptide and deionized water; the mass ratio of the arginine / lysine polypeptide to deionized water is 0.003:99.997.

[0057] The anti-aging cell chip includes sodium mannose phosphate, mannose, glycerol and deionized water; the mass ratio of sodium mannose phosphate, mannose, glycerol and deionized water is 4:2:55:50.

[0058] The composite ergothioneine comprises ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol; the mass ratio of the ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol is 0.03:96:5:0.1.

[0059] The colis peptide includes hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin; the mass ratio of the hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin is 0.3:40:80:0.5:0.1.

[0060] As a preferred technical solution of the present invention, the youth yeast includes a bifid yeast fermentation product lysate, glycerol, and 1,2-pentanediol; the mass ratio of the bifid yeast fermentation product lysate, glycerol, and 1,2-pentanediol is 80:25:8.

[0061] The life element comprises cornflower flower extract and methylpropanediol; the mass ratio of the cornflower flower extract and methylpropanediol is 47:70.

[0062] The preparation method of the mussel extract comprises the following steps: washing fresh mussels, freeze-drying them and grinding them into powder; using supercritical fluid extraction equipment and carbon dioxide as a supercritical fluid solvent, pressurizing the carbon dioxide at 32° C. to 78 bar to a supercritical state; placing the mussel powder in an extraction kettle, introducing supercritical carbon dioxide to dissolve the fat-soluble components in the mussels, and obtaining the mussel extract by pressure reduction, separation, concentration and purification.

[0063] The method for preparing the mussel anti-aging essence comprises the following steps:

[0064] (1) adding deionized water and xanthan gum to a stirring container and stirring evenly, then sequentially adding compound ergothioneine, tetrahydromethylpyrimidine carboxylic acid, life element, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol and mussel extract, and uniformly dispersing to form an aqueous phase;

[0065] (2) Add lysine polypeptide liquid, kellis peptide and youth yeast into another stirring container and mix evenly to form a polypeptide yeast mixture;

[0066] (3) adding the polypeptide yeast mixture to the aqueous phase and mixing evenly;

[0067] (4) adding the anti-aging cell chip and dimethyl isosorbide to mix evenly, adjusting the pH to 6.5 with citric acid, and homogenizing the essence with a high shear homogenizer to prepare a mussel anti-aging essence.

[0068] Example 4

[0069] A mussel anti-aging essence comprises the following raw materials in parts by weight: 1 part of lysine polypeptide liquid, 0.2 parts of anti-aging cell chips, 2 parts of compound ergothioneine, 7 parts of ectoine, 1 part of colis peptide, 1 part of youth yeast, 10 parts of isosorbide anhydride dimethyl ether, 1 part of life element, 0.01 parts of p-hydroxyacetophenone, 0.01 parts of 1,2-pentanediol, 1 part of 1,2-hexanediol, 0.2 parts of mussel extract, 0.01 parts of xanthan gum, and 50 parts of deionized water.

[0070] The polypeptide liquid is arginine / lysine polypeptide and deionized water; the mass ratio of the arginine / lysine polypeptide to deionized water is 0.002:99.998.

[0071] The anti-aging cell chip includes sodium mannose phosphate, mannose, glycerol and deionized water; the mass ratio of sodium mannose phosphate, mannose, glycerol and deionized water is 3.2:1.5:45:50.

[0072] The composite ergothioneine comprises ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol; the mass ratio of the ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol is 0.015:95:4:0.08.

[0073] The colis peptide includes hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin; the mass ratio of the hexapeptide-9, butylene glycol, water, caprylyl glycol, and ethylhexylglycerin is 0.15:22:65:0.5:0.07.

[0074] As a preferred technical solution of the present invention, the youth yeast includes a bifid yeast fermentation product lysate, glycerol, and 1,2-pentanediol; the mass ratio of the bifid yeast fermentation product lysate, glycerol, and 1,2-pentanediol is 75:18:6.

[0075] The life element comprises cornflower flower extract and methylpropanediol; the mass ratio of the cornflower flower extract and methylpropanediol is 38:55.

[0076] The preparation method of the mussel extract comprises the following steps: washing fresh mussels, freeze-drying them and grinding them into powder; using supercritical fluid extraction equipment and carbon dioxide as a supercritical fluid solvent, pressurizing the carbon dioxide at 75 bar at 30° C. to a supercritical state; placing the mussel powder in an extraction kettle, introducing supercritical carbon dioxide to dissolve the fat-soluble components in the mussels, and obtaining the mussel extract by pressure reduction, separation, concentration and purification.

[0077] The method for preparing the mussel anti-aging essence comprises the following steps:

[0078] (1) adding deionized water and xanthan gum to a stirring container and stirring evenly, then sequentially adding compound ergothioneine, tetrahydromethylpyrimidine carboxylic acid, life element, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol and mussel extract, and uniformly dispersing to form an aqueous phase;

[0079] (2) Add lysine polypeptide liquid, kellis peptide and youth yeast into another stirring container and mix evenly to form a polypeptide yeast mixture;

[0080] (3) adding the polypeptide yeast mixture to the aqueous phase and mixing evenly;

[0081] (4) adding the anti-aging cell chip and dimethyl isosorbide to mix evenly, adjusting the pH to 5.5 with citric acid, and homogenizing the essence with a high shear homogenizer to prepare a mussel anti-aging essence.

[0082] Comparative Example 1

[0083] On the basis of Example 1, the preparation method of mussel extract was changed to: fresh mussels were washed, freeze-dried and ground into powder, extracted in anhydrous ethanol for 12 hours, filtered, distilled under reduced pressure and freeze-dried to obtain mussel extract, and the rest was consistent with Example 1.

[0084] Comparative Example 2

[0085] On the basis of Example 1, no lysine polypeptide liquid was added, the added amount of Collis peptide was 18 parts by weight, and the rest was consistent with Example 1.

[0086] Comparative Example 3

[0087] On the basis of Example 1, no cres peptide was added, the added amount of youth yeast was 4 parts by weight, and the rest was consistent with Example 1.

[0088] Comparative Example 4

[0089] On the basis of Example 1, no youth yeast was added, the added amount of lysine polypeptide liquid was 18 parts by weight, and the rest was the same as Example 1.

[0090] Comparative Example 5

[0091] On the basis of Example 1, ergothioneine was not added to the composite ergothioneine, the mass ratio of Agaricus matsutake extract, 1,2-pentanediol, and ethylhexylglycerol was changed to 12.5:40:7, and the rest was consistent with Example 1.

[0092] Comparative Example 6

[0093] On the basis of Example 1, no Tricholoma matsutake extract was added to the composite ergothioneine, and the mass ratio of ergothioneine, 1,2-pentanediol, and ethylhexylglycerol was changed to 12.5:40:7, and the rest was consistent with Example 1.

[0094] Comparative Example 7

[0095] On the basis of Example 1, no anti-aging cell chip was added, the addition amount of the composite ergothioneine was changed to 4.3 parts by weight, and the rest was consistent with Example 1.

[0096] Performance Test:

[0097] The evaluation of the anti-aging effect of cosmetics includes the change in skin elasticity. The German CK Skin Elasticity Tester (MPA580) is used to measure the change in the elasticity value of the subject's facial skin. The higher the value, the better the skin elasticity.

[0098] 110 volunteers aged between 25 and 60 were selected and randomly divided into 11 groups for skin elasticity test. Before the test, the skin of each subject was tested and the skin elasticity index value was recorded. Each subject was asked to apply the anti-aging essence prepared in this specific embodiment and the comparative example once in the morning and once in the evening. During this period, except for cleansing, no other cosmetics were allowed to be used. Two months after the test, the skin of each subject was tested again, and the skin elasticity index value was recorded. The skin elasticity index increase rate of each group of volunteers was calculated, and the results are shown in the following table.

[0099] Skin elasticity index increase rate / % Example 1 35.4 Example 2 33.1 Example 3 33.9 Example 4 34.2 Comparative Example 1 20.5 Comparative Example 2 23.7 Comparative Example 3 22.6 Comparative Example 4 23.2 Comparative Example 5 21.5 Comparative Example 6 22.8 Comparative Example 7 20.3

[0100] From the test results, it can be seen that the present invention obtains a significant anti-aging effect through supercritical carbon dioxide extraction of mussels, composite lysine polypeptide liquid, corliss peptide, youth yeast, composite ergothioneine and anti-aging cell chip.

[0101] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any indirect modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A mussel anti-aging essence, characterized in that: The invention comprises the following raw materials in parts by weight: 1-32 parts of polypeptide liquid, 0.2-5 parts of anti-aging cell chips, 0.5-2 parts of compound ergothioneine, 0.1-7 parts of tetrahydromethylpyrimidine carboxylic acid, 1-3 parts of colis peptide, 1-3 parts of youth yeast, 0.2-10 parts of isosorbide anhydride dimethyl ether, 0.1-1 parts of life element, 0.01-1 parts of p-hydroxyacetophenone, 0.01-1 parts of 1,2-pentanediol, 0.01-1 parts of 1,2-hexanediol, 0.001-0.2 parts of mussel extract, 0.01-20 parts of xanthan gum and 50-97 parts of deionized water.

2. The mussel anti-aging essence according to claim 1, characterized in that: The polypeptide liquid is arginine / lysine polypeptide and deionized water; the mass ratio of the arginine / lysine polypeptide and deionized water is 0.002-0.003:99.997-99.

998.

3. The mussel anti-aging essence according to claim 1, characterized in that: The anti-aging cell chip comprises sodium mannose phosphate, mannose, glycerol and deionized water; the mass ratio of sodium mannose phosphate, mannose, glycerol and deionized water is 3-4:1-2:40-60:40-50.

4. The mussel anti-aging essence according to claim 1, characterized in that: The composite ergothioneine comprises ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol; the mass ratio of the ergothioneine, agaricus pine extract, 1,2-pentanediol and ethylhexylglycerol is 0.01-0.03:95-96:3-5:0.05-0.

1.

5. The mussel anti-aging essence according to claim 1, characterized in that: The colis peptide comprises hexapeptide-9, butylene glycol, water, caprylyl glycol and ethylhexylglycerin; the mass ratio of the hexapeptide-9, butylene glycol, water, caprylyl glycol and ethylhexylglycerin is 0.1-0.3:20-40:60-80:0.3-0.5:0.05-0.

1.

6. The mussel anti-aging essence according to claim 1, characterized in that: The youth yeast comprises a bifid yeast fermentation product lysate, glycerol and 1,2-pentanediol; the mass ratio of the bifid yeast fermentation product lysate, glycerol and 1,2-pentanediol is 70-80:15-25:3-8.

7. The mussel anti-aging essence according to claim 1, characterized in that: The life element comprises cornflower flower extract and methylpropanediol; the mass ratio of the cornflower flower extract and methylpropanediol is 30-50:50-70.

8. The mussel anti-aging essence according to claim 1, characterized in that: The preparation method of the mussel extract comprises the following steps: washing fresh mussels, freeze-drying them and grinding them into powder; using supercritical fluid extraction equipment and carbon dioxide as a supercritical fluid solvent, pressurizing the carbon dioxide at 30±2° C. to 75-80 bar to a supercritical state; placing the mussel powder in an extraction kettle, introducing supercritical carbon dioxide to dissolve fat-soluble components in the mussels, and obtaining the mussel extract by pressure reduction, separation, concentration and purification.

9. A method for preparing the mussel anti-aging essence according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) adding deionized water and xanthan gum to a stirring container and stirring evenly, then sequentially adding compound ergothioneine, tetrahydromethylpyrimidine carboxylic acid, life element, p-hydroxyacetophenone, 1,2-pentanediol, 1,2-hexanediol and mussel extract, and uniformly dispersing to form an aqueous phase; (2) Add lysine polypeptide liquid, kellis peptide and youth yeast into another stirring container and mix evenly to form a polypeptide yeast mixture; (3) adding the polypeptide yeast mixture to the aqueous phase and mixing evenly; (4) adding the anti-aging cell chip and dimethyl isosorbide to mix evenly, adjusting the pH to 5.0-6.5 with citric acid, and homogenizing the essence with a high shear homogenizer to prepare a mussel anti-aging essence.