Chinese herbal medicine bath active matter extraction process

Abstract

The invention relates to the technical field of natural medicine extraction, and discloses a herbal medicine bath active matter extraction process which comprises the following steps: step 1, raw material selection and pretreatment; step 2, fine crushing operation; step 3, efficient enzymolysis-assisted extraction; step 4, strong ultrasonic-assisted extraction; step 5, filtering and separating; step 6, vacuum concentration; and 7, performing vacuum drying to obtain a finished product. According to the herbal medicine bath active matter extraction process, the extraction rate of active ingredients is greatly increased through compound enzyme and ultrasonic synergistic extraction, temperature and pressure are strictly controlled in the links of raw material pretreatment, concentration and drying, heat-sensitive ingredients are effectively reserved, meanwhile, the purity of the extract is guaranteed through multi-layer filtration, large-scale industrial production is facilitated, and the process is suitable for industrial production. The development of the herbal medicine bath industry is powerfully promoted, and a more efficient, safer and high-quality herbal medicine bath active matter extraction scheme is provided.

Description

Technical Field

[0001] The invention relates to the technical field of natural medicine extraction, in particular to a process for extracting active substances from herbal medicinal baths. Background Art

[0002] As people's health awareness increases, herbal baths are gaining more and more attention as a traditional health care and treatment method. Herbal baths absorb the active ingredients in herbs through the skin, playing a variety of functions such as dredging meridians, removing wind and dampness, and reducing inflammation and sterilization.

[0003] Existing herbs need to extract active ingredients for the following reasons: first, in their natural state, the active ingredients of herbs are often wrapped by structures such as cell walls. When used directly in medicinal baths, it is difficult for the skin to effectively absorb these ingredients, and the medicinal effects of the herbs cannot be fully exerted; second, various impurities such as soil, sand, and microorganisms will be mixed into the herbs during their growth process. These impurities not only affect the user experience of the medicinal bath, may cause adverse reactions such as skin allergies, but also interfere with the release and absorption of active ingredients; thirdly, the active ingredient content and properties of different herbs are different. By extracting active ingredients, the effective ingredients can be accurately enriched, and medicinal bath products with targeted effects can be formulated according to different health care and treatment needs.

[0004] However, existing methods for extracting active ingredients from herbal baths have many shortcomings. Although the traditional boiling method is simple and easy, the high temperature can easily destroy the heat-sensitive active ingredients in the herbs, resulting in reduced efficacy; the organic solvent extraction method may leave residual organic solvents, posing a safety hazard and is relatively costly; the supercritical fluid extraction method has expensive equipment and complex operation, and is not suitable for large-scale extraction of active ingredients from herbal baths.

[0005] Therefore, a process for extracting active substances from herbal medicinal baths is proposed. Summary of the invention

[0006] 1. Technical issues to be resolved

[0007] In view of the shortcomings of the prior art, the present invention provides a process for extracting active ingredients from herbal baths, which greatly improves the extraction rate of active ingredients through the synergistic extraction of composite enzymes and ultrasound, strictly controls temperature and pressure during raw material pretreatment, concentration and drying, effectively retains heat-sensitive components, and simultaneously multi-layer filtration ensures the purity of the extract, thereby solving the problems raised by the background technology.

[0008] (II) Technical solution

[0009] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a process for extracting active ingredients from herbal medicinal baths, comprising the following steps:

[0010] Step 1: Select and pre-treat raw materials. Accurately select high-quality herbal raw materials, remove impurities, moldy and insect-infested parts, accurately weigh according to the established formula ratio, and rinse the herbal raw materials with clean water. After washing, use low-temperature drying equipment and strictly control the temperature between 30℃ and 50℃ to ensure that the moisture content of the herbs is steadily below 10%;

[0011] Step 2: Fine grinding operation: put the pre-treated herbal raw materials into the grinder, use advanced grinding technology to carefully control the grinding particle size to control it within the range of 20 to 80 mesh;

[0012] Step 3: efficient enzymatic hydrolysis-assisted extraction, adding an appropriate amount of complex enzyme preparation to the crushed herbal powder, the complex enzyme preparation including cellulase, pectinase, and protease, the mass ratio of which is 3:2:1, and the amount of the complex enzyme preparation added is 0.5%-2% of the mass of the herbal powder, then adding an appropriate amount of distilled water to make the water content of the material reach 40%-60%, adjusting the pH value to 4.5-6.5, and carrying out enzymatic hydrolysis under this condition, the temperature is controlled at 40°C-50°C, and the reaction time is 2-4 hours;

[0013] Step 4: strong ultrasonic assisted extraction, the enzymatically hydrolyzed material is smoothly transferred to the ultrasonic extraction equipment, distilled water is added in a mass ratio of 1:8 to 1:12, and then the ultrasonic equipment is turned on, the ultrasonic frequency is accurately set between 20 and 40 kHz, the power is stabilized in the range of 200 to 500 W, and the ultrasonic extraction process is continued for 30 to 60 minutes;

[0014] Step 5: Filtration and separation: After the ultrasonic extraction is completed, the material is filtered through a multi-layer filtration device while it is hot;

[0015] Step 6: vacuum concentration: in an advanced vacuum concentration device, the temperature is precisely controlled between 40°C and 60°C, and the pressure is stably maintained within the range of -0.08 to -0.06MPa, and the filtrate is efficiently concentrated to 1 / 5 to 1 / 3 of the original volume;

[0016] Step 7: Vacuum drying to obtain the finished product. The concentrated extract is transferred to a vacuum drying oven. The temperature is strictly controlled between 30°C and 40°C, and the vacuum degree is maintained within the range of -0.09 to -0.07MPa. The drying process is carried out for 6 to 12 hours to finally dry the extract into a powder or paste-like finished product.

[0017] Preferably, the Chinese herbal raw materials in step 1 are selected from two or more of Artemisia annua, Saposhnikovia divaricata, Herba Polygoni Multiflori, Phellodendron chinense, Sophora flavescens and Fructus Cnidium monnieri, and the wind speed of the low-temperature drying equipment should be controlled at 0.5-1.5 m / s to ensure that the herbs are evenly heated, prevent local overheating from causing damage to the active ingredients of the herbs, and further ensure the quality of the raw materials.

[0018] Preferably, the advanced pulverizing technology in step 2 utilizes high-speed airflow to cause material particles to collide and rub against each other to achieve pulverization. The pretreated herbal raw materials are fed into the pulverizing chamber through a feeding device. In the pulverizing chamber, the high-speed airflow carries the material particles to move at high speed, and strong collision, shearing and friction occur between the particles and between the particles and the wall of the pulverizing chamber, thereby pulverizing the material. At the same time, the pulverizing particle size is controlled by adjusting the pressure, flow rate and pulverizing time of the airflow.

[0019] Preferably, the mass ratio of the material to distilled water in step three is 1:10-1:12.

[0020] Preferably, in the ultrasound-assisted extraction of step four, a magnetic field or an electric field is applied simultaneously, the magnetic field strength is 0.1-1.0T, and the electric field strength is 10-100kV / m. The energy field is used to enhance the extraction efficiency and activity of the active ingredients, promote the migration and diffusion of intracellular substances, and improve the extraction rate. Moreover, this energy field assistance will not have an adverse effect on the chemical structure and properties of the active ingredients.

[0021] Preferably, the multi-layer filtration device in step five includes a 200-mesh stainless steel filter, a non-woven filter layer and an activated carbon filter layer in sequence, the 200-mesh stainless steel filter is used to initially intercept larger particle impurities, the non-woven filter layer further filters fine particles, and the activated carbon filter layer absorbs residual pigments, odors and some impurities.

[0022] Preferably, in the reduced pressure concentration step of step six, an antioxidant, a preservative or a stabilizer is added, the amount of the antioxidant added is 0.1%-0.5% of the mass of the extract, the amount of the preservative added is 0.05%-0.2% of the mass of the extract, and the amount of the stabilizer added is 0.05%-0.3% of the mass of the extract, so as to prevent the active ingredients from being oxidized, deteriorated or decomposed during the extraction process, and ensure the stability and shelf life of the final medicinal bath product.

[0023] Preferably, after the vacuum drying into the finished product in step seven, the product is subjected to strict quality inspection and then properly stored so as to be subsequently used in the production of medicinal bath products, thereby providing high-quality herbal extract raw materials for the medicinal bath products.

[0024] Preferably, the quality inspection is performed on the final powdered or paste-like finished product by high performance liquid chromatography, gas chromatography or analytical methods to inspect the quality of the active ingredients, to ensure that the content of the active ingredients in the extract reaches a predetermined standard, to ensure the consistency of the quality and efficacy of the product, and to establish corresponding quality standards and inspection indicators for different types of herbal active ingredients.

[0025] (III) Beneficial effects

[0026] Compared with the prior art, the present invention provides a process for extracting active ingredients from herbal medicinal baths, which has the following beneficial effects:

[0027] 1. In terms of extraction efficiency, the herbal bath active ingredient extraction process adopts a composite enzyme preparation to assist in extraction. The synergistic effect of cellulase, pectinase and protease can be used to specifically decompose the corresponding components of the herbal cell wall, making it easier to release the active ingredients in the cells. Combined with ultrasound-assisted extraction, the ultrasonic cavitation effect and mechanical vibration further break up the cell structure and enhance the dissolution effect of the active ingredients. Compared with the traditional single extraction method, this combined extraction method greatly improves the extraction rate of active ingredients, and can increase the extraction efficiency by 30%-50%, ensuring the efficient use of herbal resources.

[0028] 2. The process of extracting active ingredients from herbal medicinal baths is carried out by low-temperature drying during raw material pretreatment, with the temperature controlled at 30℃-50℃, effectively avoiding the damage of heat-sensitive active ingredients to high temperature. At the same time, during the process of reduced pressure concentration, the temperature is precisely maintained at 40℃-60℃, the pressure is stabilized at -0.08 to -0.06MPa, and the temperature is 30℃-40℃ and the vacuum degree is -0.09 to -0.07MPa during vacuum drying, all of which minimize the loss of active ingredients during the processing, completely retain the medicinal value of the herbs, and ensure the significant therapeutic effect of the medicinal bath products.

[0029] 3. In the process of extracting active ingredients from herbal medicinal baths, the multi-layer filtration device plays a key role in product quality assurance. The 200-mesh stainless steel filter, non-woven filter layer and activated carbon filter layer filter the extract in turn, from the initial interception of large particles of impurities, to the further removal of fine particles, and then to the adsorption of residual pigments, odors and some impurities, which fully ensures the purity of the extract, not only improves the appearance quality of the product, but more importantly, avoids the adverse effects of impurities on the medicinal bath effect, and improves the stability and safety of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The present invention provides a flow chart of a process for extracting active ingredients from herbal medicinal baths. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] See also Figure 1 , a process for extracting active ingredients from herbal medicinal baths, comprising the following steps:

[0033] Step 1: Raw material selection and pretreatment. Accurately select high-quality herbal raw materials, remove impurities, moldy and worm-eaten parts, accurately weigh according to the established formula ratio, rinse the herbal raw materials thoroughly with clean water, and use low-temperature drying equipment after washing. The temperature is strictly controlled between 30℃ and 50℃ to ensure that the moisture content of the herbs is steadily below 10%. The herbal raw materials are selected from more than two of the following: Artemisia annua, Saposhnikovia divaricata, Radix schizonepetae, Phellodendron chinense, Sophora flavescens and Cnidium monnieri, and the wind speed of the low-temperature drying equipment should be controlled at 0.5-1.5m / s to ensure that the herbs are evenly heated to prevent local overheating from damaging the active ingredients of the herbs, further ensuring the quality of the raw materials.

[0034] Step 2: Fine crushing operation. Put the pretreated herbal raw materials into the crusher. Use advanced crushing technology to carefully control the crushing particle size so that it is controlled within the range of 20 to 80 mesh. The advanced crushing technology uses high-speed airflow to make the material particles collide and rub against each other to achieve crushing. The pretreated herbal raw materials are fed into the crushing chamber through the feeding device. In the crushing chamber, the high-speed airflow carries the material particles to move at high speed, and strong collision, shearing and friction occur between the particles and between the particles and the wall of the crushing chamber, so that the material is crushed. At the same time, the crushing particle size is controlled by adjusting the pressure, flow rate and crushing time of the airflow.

[0035] Step three, efficient enzymatic hydrolysis assisted extraction, add an appropriate amount of complex enzyme preparation to the crushed herbal powder, the complex enzyme preparation includes cellulase, pectinase, and protease, the mass ratio of which is 3:2:1, and the amount of the complex enzyme preparation added is 0.5%-2% of the mass of the herbal powder, then add an appropriate amount of distilled water, the mass ratio of the material to distilled water is 1:10-1:12, so that the water content of the material reaches 40%-60%, adjust the pH value to 4.5-6.5, and carry out enzymatic hydrolysis under this condition, the temperature is controlled at 40℃-50℃, and the reaction time is 2-4 hours.

[0036] Step 4: strong ultrasonic-assisted extraction. The enzymatically hydrolyzed material is smoothly transferred to the ultrasonic extraction equipment, distilled water is added in a mass ratio of 1:8 to 1:12, and then the ultrasonic equipment is turned on. The ultrasonic frequency is accurately set between 20 and 40 kHz, and the power is stabilized in the range of 200 to 500 W. The ultrasonic extraction process is continued for 30 to 60 minutes. At the same time, a magnetic field or electric field is applied with a magnetic field strength of 0.1-1.0 T and an electric field strength of 10-100 kV / m. The extraction efficiency and activity of the active ingredients are enhanced through the action of the energy field, the migration and diffusion of intracellular substances are promoted, and the extraction rate is improved. This energy field assistance will not have an adverse effect on the chemical structure and properties of the active ingredients.

[0037] Step 5, filtration and separation treatment. After the ultrasonic extraction is completed, the material is filtered while hot through a multi-layer filtration device. The multi-layer filtration device includes a 200-mesh stainless steel filter, a non-woven filter layer and an activated carbon filter layer in sequence. The 200-mesh stainless steel filter is used to initially intercept larger particles of impurities, the non-woven filter layer further filters fine particles, and the activated carbon filter layer absorbs residual pigments, odors and some impurities.

[0038] Step six, vacuum concentration. In an advanced vacuum concentration device, the temperature is precisely controlled between 40°C and 60°C, and the pressure is stably maintained in the range of -0.08 to -0.06MPa. The filtrate is efficiently concentrated to 1 / 5 to 1 / 3 of the original volume. In the vacuum concentration step, antioxidants, preservatives or stabilizers are added. The amount of antioxidant added is 0.1%-0.5% of the mass of the extract, the amount of preservative added is 0.05%-0.2% of the mass of the extract, and the amount of stabilizer added is 0.05%-0.3% of the mass of the extract to prevent the active ingredients from being oxidized, deteriorated or decomposed during the extraction process, thereby ensuring the stability and shelf life of the final medicinal bath product.

[0039] Step seven, vacuum drying into a finished product, transfer the concentrated extract to a vacuum drying oven, strictly control the temperature between 30°C and 40°C, maintain the vacuum degree in the range of -0.09 to -0.07MPa, and perform drying treatment for 6 to 12 hours to finally dry the extract into a powder or paste-like finished product. After the product undergoes strict quality inspection, the finished product is properly stored for subsequent application in the production of medicinal bath products, providing high-quality herbal extract raw materials for medicinal bath products. The quality inspection is performed on the final powder or paste product by high-performance liquid chromatography, gas chromatography or analytical methods to perform quality inspection of active ingredients to ensure that the content of active ingredients in the extract meets the predetermined standard, ensure the quality and efficacy consistency of the product, and establish corresponding quality standards and detection indicators for different types of herbal active substances.

[0040] In summary, wormwood and siler were selected as herbal raw materials, impurities, mildew and insect-eaten parts were removed respectively, a total of 800 g was weighed at a mass ratio of 2:1, and after being rinsed with clean water, they were placed in a low-temperature drying equipment with good ventilation and a temperature set at 40°C for drying, and the wind speed was controlled at 1 m / s to ensure that the herbs were evenly heated and the water content was reduced to 8%; the dried herbal raw materials were placed in a grinder using high-speed airflow pulverizing technology, and the pulverized particle size was controlled at 50 mesh by adjusting the airflow pressure to 0.8 MPa, the flow rate to 40 m / s and the pulverizing time to 8 minutes; a composite enzyme preparation (cellulose) with a mass of 1.2% of the herbal powder was added to the pulverized herbal powder. The mass ratio of enzyme, pectinase and protease is 3:2:1), distilled water is added to make the water content of the material reach 50%, the pH value is adjusted to 5.5, and the enzymolysis reaction is carried out at 45°C for 3 hours; after the enzymolysis is completed, the material is transferred to an ultrasonic extraction device, distilled water is added according to a mass ratio of 1:10, the ultrasonic device is turned on, and a magnetic field is applied at the same time, the magnetic field strength is 0.5T, the ultrasonic frequency is set to 30kHz, the power is 350W, and the ultrasonic extraction is carried out for 45 minutes; after the ultrasonic extraction is completed, the material is passed through a multi-layer filtration device (200 mesh stainless steel filter, non-woven filter layer and activated carbon filter layer in sequence, the activated carbon filter layer has an activated carbon particle size of 1mm and a specific surface area of ​​900m 2 / g) is filtered, the filtrate is collected and transferred to a reduced pressure concentration device, and at 50°C and a pressure of -0.07MPa, an antioxidant (added in an amount of 0.3% of the mass of the extract), a preservative (added in an amount of 0.1% of the mass of the extract) and a stabilizer (added in an amount of 0.2% of the mass of the extract) are added to the filtrate, and the filtrate is concentrated to 1 / 4 of the original volume; finally, the concentrated extract is transferred to a vacuum drying oven, and dried at 35°C and a vacuum degree of -0.08MPa for 9 hours to obtain a powdered product, and the finished product is tested for active ingredients by high performance liquid chromatography, and the results show that the contents of the main active ingredients of wormwood and siler reach the predetermined standards, the product quality is qualified, and can be used for subsequent preparation of medicinal bath products.

[0041] The finished products obtained above were subjected to active ingredient detection and clinical trials on the medicinal bath effect. The results showed that the herbal medicinal bath active substances extracted by the process of the present invention have high active ingredient contents, and have significant improvement effects on symptoms such as skin itching and joint pain after medicinal bathing, without any adverse reactions, which verifies the effectiveness and safety of the process of the present invention.

[0042] It should be noted that the term "comprises" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, article, or device that includes a series of elements includes not only those elements, but also includes other elements that are not explicitly listed, or also includes elements inherent to such process, method, article, or device. In the absence of further restrictions, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article, or device that includes the element.

[0043] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A process for extracting active ingredients from herbal medicinal baths, characterized in that: The following steps are involved: Step 1: Select and pre-treat raw materials. Accurately select high-quality herbal raw materials, remove impurities, moldy and insect-infested parts, accurately weigh according to the established formula ratio, and rinse the herbal raw materials with clean water. After washing, use low-temperature drying equipment and strictly control the temperature between 30℃ and 50℃ to ensure that the moisture content of the herbs is steadily below 10%; Step 2: Fine grinding operation: put the pre-treated herbal raw materials into the grinder, use advanced grinding technology to carefully control the grinding particle size to control it within the range of 20 to 80 mesh; Step 3: efficient enzymatic hydrolysis-assisted extraction, adding an appropriate amount of complex enzyme preparation to the crushed herbal powder, the complex enzyme preparation including cellulase, pectinase, and protease, the mass ratio of which is 3:2:1, and the amount of the complex enzyme preparation added is 0.5%-2% of the mass of the herbal powder, then adding an appropriate amount of distilled water to make the water content of the material reach 40%-60%, adjusting the pH value to 4.5-6.5, and carrying out enzymatic hydrolysis under this condition, the temperature is controlled at 40°C-50°C, and the reaction time is 2-4 hours; Step 4: strong ultrasonic assisted extraction, the enzymatically hydrolyzed material is smoothly transferred to the ultrasonic extraction equipment, distilled water is added in a mass ratio of 1:8 to 1:12, and then the ultrasonic equipment is turned on, the ultrasonic frequency is accurately set between 20 and 40 kHz, the power is stabilized in the range of 200 to 500 W, and the ultrasonic extraction process is continued for 30 to 60 minutes; Step 5: Filtration and separation: After the ultrasonic extraction is completed, the material is filtered through a multi-layer filtration device while it is hot; Step 6: vacuum concentration: in an advanced vacuum concentration device, the temperature is precisely controlled between 40°C and 60°C, and the pressure is stably maintained within the range of -0.08 to -0.06MPa, and the filtrate is efficiently concentrated to 1 / 5 to 1 / 3 of the original volume; Step 7: Vacuum drying to obtain the finished product. The concentrated extract is transferred to a vacuum drying oven. The temperature is strictly controlled between 30°C and 40°C, and the vacuum degree is maintained within the range of -0.09 to -0.07MPa. The drying process is carried out for 6 to 12 hours to finally dry the extract into a powder or paste-like finished product.

2. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: In the step 1, the Chinese herbal medicine raw materials are selected from two or more of Artemisia argyi, Saposhnikovia divaricata, Herba Polygoni Multiflori, Phellodendron amurense, Sophora flavescens and Cnidium monnieri, and the wind speed of the low-temperature drying equipment should be controlled at 0.5-1.5 m / s.

3. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: The advanced pulverizing technology in the step 2 utilizes high-speed airflow to cause the material particles to collide and rub against each other to achieve pulverization. The pretreated herbal raw materials are fed into the pulverizing chamber through a feeding device. In the pulverizing chamber, the high-speed airflow carries the material particles to move at high speed, and strong collision, shearing and friction occur between the particles and between the particles and the wall of the pulverizing chamber, thereby pulverizing the material. At the same time, the pulverizing particle size is controlled by adjusting the pressure, flow rate and pulverizing time of the airflow.

4. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: The mass ratio of the material to distilled water in the step 3 is 1:10-1:

12.

5. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: In the ultrasound-assisted extraction of step 4, a magnetic field or an electric field is applied simultaneously, the magnetic field strength is 0.1-1.0T, and the electric field strength is 10-100kV / m.

6. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: The multi-layer filtration device in step five includes a 200-mesh stainless steel filter, a non-woven filter layer and an activated carbon filter layer in sequence. The 200-mesh stainless steel filter is used to initially intercept larger particle impurities, the non-woven filter layer further filters fine particles, and the activated carbon filter layer absorbs residual pigments, odors and some impurities.

7. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: In the reduced pressure concentration step of step six, an antioxidant, a preservative or a stabilizer is added, wherein the amount of the antioxidant added is 0.1%-0.5% of the mass of the extract, the amount of the preservative added is 0.05%-0.2% of the mass of the extract, and the amount of the stabilizer added is 0.05%-0.3% of the mass of the extract.

8. The process for extracting active ingredients from herbal medicinal baths according to claim 1, characterized in that: After the vacuum drying into the finished product in step seven, the product is subjected to strict quality inspection and then properly stored for subsequent use in the production of medicinal bath products, thereby providing high-quality herbal extract raw materials for the medicinal bath products.

9. The process for extracting active ingredients from herbal medicinal baths according to claim 8, characterized in that: The quality inspection is to inspect the quality of active ingredients in the final powdered or paste-like finished product by high performance liquid chromatography, gas chromatography or analytical methods, and to establish corresponding quality standards and inspection indicators for different types of herbal active substances.

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