Application of herba selaginellae in promoting skin healing

Abstract

The invention provides an application of herba selaginellae in promoting skin healing. Tests show that the herba selaginellae powder and the herba selaginellae extract not only can promote wound scabbing through oral administration, but also can be prepared into dressings to promote wound scabbing through external application, and the skin is smooth and soft after being healed; in addition, the herba selaginellae powder, the herba selaginellae extract and the externally prepared gel can reduce the level of inflammatory factors, improve the level of superoxide dismutase, reduce vascular endothelial growth factors and epidermal growth factors to relieve the influence on scar formation, and have an obvious treatment effect of promoting skin healing. Moreover, compared with an oral medicine, the surface smearing medicine has more advantages, the smearing medicine can directly act on the local part of the wound, the concentration of the medicine reaches a high level at the wound, a better treatment effect is achieved, and the smearing medicine can rapidly relieve the symptoms such as wound pain and red swelling.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an application of Amomum villosum in promoting skin healing. Background Art

[0002] As the first protective barrier of the body, the skin is extremely vulnerable to trauma. Skin wound healing refers to the healing process after the skin tissue is severed or defective due to external force. Based on previous clinical practice experience, in the healing process of wounds, incisions and various anastomoses of patients with trauma or surgery, poor healing may lead to wound or incision infection and incision dehiscence. For example, poor healing of digestive tract anastomosis may cause complications such as intestinal leakage, pancreatic leakage or bile leakage. In mild cases, it will reduce the actual effect of surgical treatment, and in severe cases, it will directly threaten the patient's life safety. Therefore, treatments that promote the healing of various wounds play a vital role in human life and health. Wound healing is a complex biological process and a key factor in restoring the integrity of the skin barrier. However, the current treatment methods mainly include iodine disinfection, hydrogen peroxide and saline flushing, and dressing coverage, which cannot effectively promote skin healing. Summary of the invention

[0003] In view of this, the technical problem to be solved by the present invention is to provide an application of Herba Astragali in promoting skin healing.

[0004] The invention provides an application of Amomum villosum in preparing a product for promoting skin healing.

[0005] In a specific embodiment provided by the present invention, the product is a medicine or a dressing; the medicine can be an oral medicine or an external medicine; the dressing can be any dressing well known to those skilled in the art without any special limitation, and is preferably a gel in the present invention.

[0006] In a specific embodiment provided by the present invention, the product includes Herba Aspergillum powder and / or Herba Aspergillum extract.

[0007] In a specific embodiment provided by the present invention, the Amomum villosum powder is preferably obtained by crushing the dried whole herb of Amomum villosum and then sieving it; the sieving preferably uses a sieve No. 4 to 8, more preferably a sieve No. 6 to 8, and even more preferably a sieve No. 7.

[0008] In a specific embodiment provided by the present invention, the Herba Lycopodii extract is preferably a Herba Lycopodii water extract and / or a Herba Lycopodii ethanol extract.

[0009] In a specific embodiment provided by the present invention, the water extract of Ampelopsis pilosula is prepared according to the following method: A1) extracting Ampelopsis pilosula by heating and refluxing with water to obtain an extract; A2) drying the extract to obtain the water extract of Ampelopsis pilosula.

[0010] In a specific embodiment provided by the present invention, the step A1) is specifically to extract the dried whole herb of Amomum villosum by heating and refluxing with water to obtain an extract.

[0011] In a specific embodiment provided by the present invention, the mass ratio of the sand grass to water is preferably 1: (8-20), more preferably 1: (10-15), and even more preferably 1: 12; the number of extractions is preferably 2-4 times, more preferably 3 times; the time for each extraction is preferably 2-6 hours, more preferably 3-5 hours, and even more preferably 4 hours; the method for drying the extract is preferably a method well known to those skilled in the art, and there is no special limitation. In the present invention, air-drying is preferred.

[0012] In a specific embodiment provided by the present invention, the Herba Lycopodii ethanol extract is prepared according to the following method: B1) extracting Herba Lycopodii with ethanol solution and heated water to obtain an extract; B2) removing the solvent in the extract to obtain the Herba Lycopodii ethanol extract.

[0013] In a specific embodiment provided by the present invention, the step B1) is specifically to extract the dried whole herb of Amomum villosum by heating and refluxing with an ethanol solution to obtain an extract.

[0014] In a specific embodiment provided by the present invention, the mass concentration of the ethanol solution is preferably 90% to 98%, more preferably 92% to 96%, and even more preferably 95%; the mass ratio of the sand-lead grass to the ethanol solution is preferably 1:(8 to 20), more preferably 1:(10 to 15), and even more preferably 1:12; the number of extractions is preferably 2 to 4 times, more preferably 3 times; the time for each extraction is preferably 2 to 6 hours, more preferably 3 to 5 hours, and even more preferably 4 hours.

[0015] In a specific embodiment provided by the present invention, the solvent in the extract can be removed by a method well known to those skilled in the art without any special limitation. In the present invention, the extract is preferably concentrated under reduced pressure and dried.

[0016] In a specific embodiment provided by the present invention, the dressing comprises, in terms of raw materials:

[0017]

[0018] The pH adjuster adjusts the pH value of the dressing to 6-8.

[0019] In a specific embodiment provided by the present invention, the dressing comprises, in terms of raw materials:

[0020]

[0021] The pH adjuster adjusts the pH of the dressing to 6 to 8;

[0022] Or, include:

[0023]

[0024] The pH adjuster adjusts the pH value of the dressing to 6-8.

[0025] In a specific embodiment provided by the present invention, the content of water in the dressing is preferably 60 to 80 parts by weight, more preferably 60 to 75 parts by weight, and even more preferably 60 to 70 parts by weight.

[0026] In a specific embodiment provided by the present invention, the content of the gel matrix in the dressing is preferably 1 to 5 parts by weight, more preferably 1.5 to 3 parts by weight; the gel matrix can be any gel matrix well known to those skilled in the art, without any special restrictions, and in the present invention, it is preferably one or more of carbomer, sodium alginate, tragacanth gum, hydroxypropyl methylcellulose and sodium carboxymethyl cellulose; in some embodiments provided by the present invention, carbomer (941) is specifically used as an example for illustration.

[0027] In a specific embodiment provided by the present invention, the content of the moisturizing agent in the dressing is preferably 8 to 15 parts by weight, more preferably 10 to 15 parts by weight, further preferably 12 to 15 parts by weight, and most preferably 12 to 13 parts by weight; the moisturizing agent can be a moisturizing agent well known to those skilled in the art without any special limitation, and is preferably glycerol and / or propylene glycol in the present invention.

[0028] In a specific embodiment provided by the present invention, the pH adjuster preferably adjusts the pH value of the dressing to 6.5-7.5, and more preferably adjusts the pH value of the dressing to 7; the pH adjuster can be a pH adjuster well known to those skilled in the art, without any special restrictions, and in the present invention, it is preferably one or more of triethanolamine, sodium hydroxide and potassium hydroxide.

[0029] In a specific embodiment provided by the present invention, the dressing comprises, in terms of raw materials:

[0030]

[0031]

[0032] The pH adjuster adjusts the pH value of the dressing to 6-8.

[0033] In a specific embodiment provided by the present invention, the dressing comprises, in terms of raw materials:

[0034]

[0035] The pH adjuster adjusts the pH value of the dressing to 6-8.

[0036] In a specific embodiment provided by the present invention, the dressing is prepared according to the following method: the powder of Amomum villosum and / or the extract of Amomum villosum is mixed with water by ultrasound, and then a gel matrix is ​​added to the mixture and allowed to stand, and then a moisturizer is added, and finally the pH value of the dressing is adjusted to 6 to 8 with a pH adjuster to obtain a dressing.

[0037] In a specific embodiment provided by the present invention, the time of the mixed ultrasound is preferably 10 to 30 minutes, more preferably 15 to 25 minutes, and even more preferably 20 minutes.

[0038] In a specific embodiment provided by the present invention, the standing time is preferably 5 to 15 hours, more preferably 5 to 12 hours, further preferably 6 to 10 hours, and most preferably 8 hours.

[0039] Compared with the prior art, the present invention provides an application of sand-leading grass in promoting skin healing. It can be seen from the experiment that sand-leading grass powder and sand-leading grass extract can not only promote wound scab by oral administration, but also be prepared into dressings to promote wound scab by external application, and the skin is smooth and soft after healing; in addition, sand-leading grass powder, sand-leading grass extract and external gel can reduce the level of inflammatory factors (IL-6, TNF-ɑ), improve the effect of superoxide dismutase (SOD) level, reduce vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) to alleviate its influence on scar formation, and have obvious therapeutic effect of promoting skin healing. Moreover, compared with oral drugs, surface application of drugs is more advantageous, and the application of drugs can directly act on the wound part, so that the drug concentration reaches a higher level at the wound, and a better therapeutic effect is exerted. The application of drugs can quickly relieve symptoms such as wound pain and redness, and oral drugs need to be absorbed and metabolized by the digestive system, resulting in a lower drug concentration at the wound, and the therapeutic effect is relatively weaker than external use. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a photo of the rat skin injury model;

[0041] Figure 2 The following are photos of animals in each group receiving trauma at different times. DETAILED DESCRIPTION

[0042] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] The reagents used in the following examples are all commercially available.

[0044] Example

[0045] 1. Experimental Methods

[0046] 1. Drug preparation:

[0047] (1) Shayincao powder: Take the dried whole grass of Shayincao, grind it with a grinder and pass it through a No. 7 sieve to obtain the powder.

[0048] (2) Alcohol extract of Herba Aspergillum: 1.5 kg of dried whole herb of Herba Aspergillum was taken and extracted with 12 times the amount of 95% ethanol under heating and reflux for 3 times, each time for 4 hours. The extract was concentrated under reduced pressure and then air-dried to obtain the ethanol extract of Herba Aspergillum.

[0049] (3) Water extract of Herba Aspergillum: Take 1.5 kg of dried whole herb of Herba Aspergillum, and extract it by heating under reflux for 3 times with 12 times the amount of distilled water, each time for 4 hours. After the extract is dried in the air, the water extract of Herba Aspergillum is obtained.

[0050] (4) Gel of Herba Cynoglossi powder: Take 4 g of prepared Herba Cynoglossi powder, add 70 mL of distilled water, and sonicate for 20 minutes; then add 3 g of Carbomer (941), mix well and let stand for 8 hours; add 10 mL of glycerol, stir well, and adjust the pH value to 7 with triethanolamine.

[0051] (5) Gel of water extract of Ampelopsis ternata: Take 0.6 g of the prepared water extract of Ampelopsis ternata, add 60 mL of distilled water, and ultrasonicate for 20 minutes; add 1.5 g of carbomer (941), mix well and let stand for 8 hours; then add 10 mL of glycerol (propylene glycol), stir well, and adjust the pH value to 7 with triethanolamine.

[0052] (6) Gel of ethanol extract of Ampelopsis pilosula: Take 0.3 g of the prepared ethanol extract of Ampelopsis pilosula, add 60 mL of distilled water, and sonicate for 20 minutes; add 1.5 g of carbomer (941), mix well and let stand for 8 hours; then add 10 mL of glycerol (propylene glycol) and stir well, and adjust the pH value to 7 with triethanolamine.

[0053] 2. Establishment of Animal Skin Wound Model and Drug Administration

[0054] (1) Selection and grouping of experimental animals:

[0055] 80 SD experimental rats (weight 180g-220g) were fed with ordinary feed, and the indoor conditions of the animals were basically controlled at a temperature of 20-26℃ and a humidity of 40%-60%. After 7 days of adaptive feeding, the experimental rats were randomly divided into a normal control group, a skin injury model group, a positive drug group, a self-healing group, a scutellaria baicalensis powder group, a scutellaria baicalensis water extract group, a scutellaria baicalensis ethanol extract group, a scutellaria baicalensis powder gel, a scutellaria baicalensis ethanol extract gel, and a scutellaria baicalensis water extract gel, with 8 rats in each group.

[0056] (2) Establishment of rat skin trauma model:

[0057] Except for the normal control group, the animals in the other experimental groups were modeled. After anesthesia (1% sodium pentobarbital injected intraperitoneally), hair shaving, and disinfection (iodine solution), the rat defect wound model was established by double-punching the back skin (two circular full-thickness defect wound models with a diameter of about 6 mm were made on the left and right sides of the back of the rat using a skin puncher), and the model was bandaged with sterile gauze. Figure 1 , Figure 1 This is a photo of the rat skin injury model.

[0058] (3) Method of administration:

[0059] After the model was successfully established, the rats were kept in separate cages. The rats in the Glehnia littoralis groups and the positive drug group were gavaged once a day (converted according to the clinical dosage), the Glehnia littoralis powder gel, the Glehnia littoralis ethanol extract gel, and the Glehnia littoralis water extract gel were applied twice a day, and the rats in the blank group were gavaged with CMC-Na for 14 consecutive days. On the second day of the experiment, blood was collected from the rats in the skin injury model group and the injury area was measured.

[0060] 3. Observation and testing:

[0061] The wounds of the experimental animals were observed every day, and the healing time, wound area, etc. were recorded to track the progress of wound healing. Photos of the wounds of each group of animals at different times were obtained. Figure 2 Serum indexes were detected and tissue changes were observed (HE staining).

[0062] (1) Take photos and calculate the wound area: (wound area = d1 × d2 × π × 1 / 4 × 2, π is 3.14) unit: mm;

[0063] (2) Detection of serum indexes: After 14 days of treatment, blood was collected through the abdominal aorta under anesthesia and centrifuged at 3000 r / min for 20 min to obtain the supernatant. The levels of SOD, TNF-α, IL-6, VEGF and EGF in the serum of rats in each group were detected by Elisa method. The specific steps were carried out according to the instructions of the kit. The results of serum indexes of rats in each group are shown in Table 2.

[0064] 2. Experimental Results

[0065] 1. Observe the effects of each group of Amomum villosum on skin healing

[0066] From Table 2, Figure 2 It can be seen that the skin injury area of ​​rats in each group was basically the same when modeling. On the 5th day after administration, the wound surface of the self-healing group was darker in color and had formed scabs; dry scabs formed on the wound surface of each drug administration group. On the 10th day after administration, the wound surfaces of all groups had scabs, and the wound surfaces of the experimental group and the positive drug group were smaller than those of the model group. The wound surface of the self-healing group was larger than that of the other groups, and the dry scabs of the positive group fell off. From the pictures, it was observed that the effect of the gel-applied drug group was better than that of the oral drug group. On the 14th day after administration, except for the self-healing group and the powder group, most of the scabs fell off; the wound surface of the gel-applied group was basically healed, and the skin of the drug-applied group was smoother and softer than that of the self-healing group.

[0067] Table 1 Skin wound area of ​​rats in each group

[0068]

[0069] Note: Compared with the spontaneous recovery group, * P<0.05, ** P<0.01.

[0070] 2. Detection of serum indexes of each group of Achyranthes bidentata

[0071] As shown in Table 2, after administration, the levels of inflammatory factors (IL-6, TNF-α), superoxide dismutase (SOD), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) all changed. The levels of IL-6, TNF-α, VEGF, and EGF in the model group and the self-healing group were higher, and the level of SOD was lower. The levels of IL-6, TNF-α, VEGF, and EGF in the herba spatholobiformis powder group, herba spatholobiformis water extract group, herba spatholobiformis alcohol extract group, herba spatholobiformis powder gel, herba spatholobiformis alcohol extract gel, herba spatholobiformis water extract gel, and the positive group were lower, and the level of SOD was higher. This indicates that after administration, inflammatory factors decreased, antioxidant capacity was enhanced, and scar formation relief was enhanced. The results were significantly different, and the treatment was effective. The various indicators of the smearing group were significantly improved compared with those of the oral group, indicating that the smearing gel was better than oral administration.

[0072] Table 2 Effects of Herba Astragali on serum parameters of rats with skin trauma

[0073]

[0074] Water extraction group: 111.205±12.68 # 232.38±38.10 # 5.00±0.81 # 230.07±31.76 329.24±44.67 #

[0075] Alcohol extraction group: 104.33±11.73 # 237.62±39.58 # 4.96±1.08 # 207.82±20.95 # 346.32±34.80 #

[0076] Powder and gel group: 93.32±15.81 ## 204.00±5.11 # 6.14±0.65 ## 206.23±38.06 # 308.53±26.37 #

[0077] Water-extracted gel group: 88.48±10.23 ## 212.43±20.41 # 6.25±0.44 ## 201.96±28.05 # 261.73±37.20 ##

[0078] Alcohol gel group 89.42±9.89 ## 207.16±18.26 # 4.92±1.12 # 199.08±27.89 # 252.41±39.29 ##

[0079] Positive drug group 87.88±15.85 ## 200.72±23.39 ## 5.31±0.65 # 177.73±13.98 # 223.91 ±48.58 ##

[0080] Note: Compared with the blank group, * P<0.05, ** P<0.01; compared with the spontaneous recovery group, # P<0.05, ## P<0.01.

[0081] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An application of Herba Astragali in preparing a product for promoting skin healing.

2. The use according to claim 1, characterized in that: The product is a medicine or a dressing; the product comprises Herba Aspergillum powder and / or Herba Aspergillum extract.

3. The use according to claim 2, characterized in that: The Herba Lycopodii extract is selected from Herba Lycopodii water extract and / or Herba Lycopodii ethanol extract.

4. The use according to claim 3, characterized in that: The water extract of Herba Aspergillume is prepared according to the following method: A1) extracting the herb by heating it under reflux with water to obtain an extract; A2) drying the extract to obtain an aqueous extract of Herba Lycopodii; And / or, the Herba Aspergillus niger ethanol extract is prepared according to the following method: B1) extracting the herb with water heated by an ethanol solution to obtain an extract; B2) removing the solvent from the extract to obtain the Herba Astragali ethanol extract.

5. The use according to claim 4, characterized in that: In step A1), the mass ratio of sand and grass to water is 1: (8-20); The number of extractions in step A1) is 2 to 4 times, and the time for each extraction is 2 to 6 hours; The mass concentration of the ethanol solution in step B1) is 90% to 98%; the mass ratio of the sand grass to the ethanol solution is 1: (8 to 20); The number of extractions in step B1) is 2 to 4 times, and the time for each extraction is 2 to 6 hours.

6. The use according to claim 2, characterized in that: In terms of raw materials, the dressing includes: The pH adjuster adjusts the pH value of the dressing to 6-8.

7. The use according to claim 6, characterized in that: In terms of raw materials, the dressing includes: The pH adjuster adjusts the pH of the dressing to 6 to 8; Or, include: The pH value adjuster adjusts the pH value of the gel to 6-8.

8. The use according to claim 6 or 7, characterized in that: In terms of raw materials, the dressing includes: The pH adjuster adjusts the pH of the dressing to 6 to 8; Or, include: The pH adjuster adjusts the pH value of the dressing to 6-8.

9. The use according to any one of claims 6 to 8, characterized in that: The gel matrix is ​​selected from one or more of carbomer, sodium alginate, tragacanth gum, hydroxypropyl methylcellulose and sodium carboxymethyl cellulose; the moisturizer is selected from glycerol and / or propylene glycol; the pH regulator is selected from one or more of triethanolamine, sodium hydroxide and potassium hydroxide.

10. The use according to claim 6, characterized in that: The dressing is prepared according to the following method: The herb powder and / or herb extract is mixed with water by ultrasonication, then a gel matrix is ​​added, the mixture is allowed to stand, a moisturizer is added, and finally a pH regulator is used to adjust the pH value of the dressing to 6-8 to obtain a dressing.

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