Preparation method and application of plant extract of mycoplasma gallisepticum

Abstract

The invention discloses a preparation method of a plant extract of mycoplasma gallisepticum, which comprises the following steps: respectively obtaining raw materials in parts by weight, crushing the raw materials, adding boiling water, and stirring into paste to obtain paste; freezing the paste at the temperature of-25 DEG C for 24 hours, and unfreezing at normal temperature to obtain a mixture; mixing the mixture with a 95% ethanol solution according to the mass ratio of 1: 3, and sequentially carrying out organic extraction and vacuum concentration under the condition of normal temperature to obtain clear paste with the density of 1.18-1.25; and mixing the clear paste with 20% acetic acid according to a mass ratio of 1: 3 to obtain the plant extract of mycoplasma gallisepticum. The raw materials are proportioned to have a synergistic effect on inhibiting or killing mycoplasma gallisepticum. The invention further discloses application of the plant extract of the mycoplasma gallisepticum, the concentration is adjusted through blending, and then the head of a chicken is subjected to atomization spraying treatment by using blended liquid medicine, so that the effect of preventing and treating the mycoplasma gallisepticum is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant extraction, and more specifically to a preparation method and application of a plant extract of Mycoplasma gallisepticum. Background Art

[0002] Mycoplasma gallisepticum is a very difficult disease in the chicken farming industry. Mycoplasma gallisepticum is a pathogenic species of the order Mycoplasma, family Mycoplasma, genus Mycoplasma. It is a very small prokaryotic organism with no cell wall and only cell membrane. The infection characteristics of Mycoplasma gallisepticum are mainly colonization on the cilia and air sacs of the tracheal mucosa, and there are no blood vessels at the ends of the tracheal cilia and air sacs. The drug is transported to the site of action through the blood and then acts on Mycoplasma gallisepticum through diffusion. Therefore, the drug can only reduce the number of Mycoplasma colonized on the tracheal cilia and air sacs and alleviate clinical symptoms, but cannot completely kill them. Therefore, Mycoplasma gallisepticum infection is generally a lifelong carrier state of persistent infection after a single infection. When the chickens are under stress, such as cold stimulation or vaccine immunization, clinical symptoms will appear again.

[0003] Another scary thing about Mycoplasma gallisepticum is that it can work together with other pathogenic factors to harm chickens. Sometimes Mycoplasma gallisepticum does not need to show signs of disease. As long as the chicken is in the latent state of Mycoplasma gallisepticum, once infected with other diseases, it will cause large-scale illness in the chickens. For example, after the chickens mature, if they are only infected with E. coli, they only need simple treatment to quickly curb the disease, and generally no deaths will occur. However, when the chickens are infected with E. coli and are also latently infected with Mycoplasma gallisepticum, the treatment time and cost will increase exponentially, and the mortality rate will range from one thousandth to five thousandths per day. It can be said that Mycoplasma gallisepticum is the source of all diseases in the poultry industry. The direct and indirect losses caused by Mycoplasma gallisepticum in the national chicken farming industry amount to tens of billions of yuan each year.

[0004] In order to prevent and treat Mycoplasma gallisepticum, drugs are usually mixed with feed or added to drinking water to deliver the active ingredients of the drugs to the lesions through blood circulation to exert a therapeutic effect. If the lesions of the chicken's respiratory tract are in the alveoli and air sacs, which are areas with few capillaries, large doses of drugs are needed to deliver the drugs to the lesions through blood circulation if they are treated by mixing feed or drinking water. Therefore, the treatment will be more difficult and the cost will be higher. If antibiotics and chemical synthetic drugs with high toxicity are used, the dosage is small and the therapeutic effect cannot be exerted. If the dosage is large, it will cause secondary damage to the poultry, while reducing the quality of poultry products. The residual drug residues will also have an adverse effect on the breeding environment.

[0005] Therefore, there is an urgent need for a plant extract of Mycoplasma gallisepticum that is green, environmentally friendly, effective, stable, and easy to use. Summary of the invention

[0006] An object of the present invention is to address at least the above-mentioned disadvantages and to provide at least the advantages which will be described hereinafter.

[0007] Another object of the present invention is to provide a method for preparing a plant extract of Mycoplasma gallisepticum.

[0008] Another object of the present invention is to provide an application of a plant extract of Mycoplasma gallisepticum.

[0009] In order to achieve these purposes and other advantages of the present invention, the present invention provides a plant extract of Mycoplasma gallisepticum, which comprises the following raw materials obtained in the following weight parts: 45-50 parts of pine needles, 30-40 parts of licorice, 30-40 parts of coptis root, 25-35 parts of phellodendron, 20-30 parts of bupleurum, 17-25 parts of dandelion, 15-20 parts of Lysimachia sibiricum, 7-15 parts of plantain, and 5-10 parts of Poria.

[0010] A method for preparing a plant extract of Mycoplasma gallisepticum comprises: The following raw materials are obtained according to the following weight portions: 45-50 parts of pine needles, 30-40 parts of licorice, 30-40 parts of coptis chinensis, 25-35 parts of phellodendron, 20-30 parts of bupleurum, 17-25 parts of dandelion, 15-20 parts of moneywort, 7-15 parts of plantain, and 5-10 parts of poria.

[0011] The raw materials are crushed and then added into boiling water and stirred into a paste to obtain a paste.

[0012] The paste was frozen at -25°C for 24 hours and then thawed at room temperature to obtain a mixture.

[0013] The mixture was mixed with 95% ethanol solution in a mass ratio of 1:3, and organic extraction and vacuum concentration were performed in sequence at room temperature to obtain a clear paste with a density of 1.18-1.25.

[0014] The clear paste was mixed with 20% acetic acid in a mass ratio of 1:3 to obtain the plant extract of Mycoplasma gallisepticum.

[0015] In the above scheme, specifically, the particle size of the raw material after crushing is 50 mesh; the organic extraction specifically includes: mixing the mixture with 95% ethanol solution in a volume ratio of 1:3, extracting at room temperature for 3 hours, repeating the filtration at least 3 times, combining the filtrate and removing the ethanol by vacuum distillation; the vacuum concentration specifically includes: placing the extract after organic extraction in a vacuum rotary instrument and concentrating it in a water bath at a constant temperature of 60°C to a relative density of 1.18~1.25; except for the freezing step, the environment of other working steps is a temperature of 18~30°C and a relative humidity of 30%~70%.

[0016] The above raw materials are mixed in a proportion to have a synergistic effect on inhibiting or killing Mycoplasma gallisepticum; the raw materials are crushed and added into boiling water and stirred into a paste, which helps the effective ingredients in the raw materials to be better dissolved in water; freeze-thaw treatment helps to change the molecular structure of the raw materials and improve the subsequent extraction efficiency; and the raw materials are mixed with acetic acid in proportion to further enhance the antibacterial activity of the extract.

[0017] The application of plant extracts of Mycoplasma gallisepticum in preparations for preventing Mycoplasma gallisepticum includes: The plant extract of Mycoplasma gallisepticum and water are mixed in a volume ratio of 1:5 to obtain a mixed solution for preventing Mycoplasma gallisepticum; Atomize and spray the chicken's head with a mixed solution for preventing Mycoplasma gallisepticum at a dose of 0.1 ml per chicken, twice a week.

[0018] In the above scheme, specifically, when spraying, the doors and windows of the chicken house and the exhaust system must be closed before spraying can be carried out. After spraying, the doors and windows and the exhaust system must continue to be closed for 30 minutes to allow the droplets to be suspended in the air of the poultry house. The poultry house can be ventilated after 30 minutes.

[0019] The plant extract of Mycoplasma gallisepticum is formulated to adjust the concentration, and then the formulated liquid medicine is used to perform atomization spray treatment on the head of the chicken, so that the liquid medicine can effectively enter the upper respiratory tract of the chicken, thereby forming a protective film on the respiratory mucosa to prevent infection with Mycoplasma gallisepticum.

[0020] The application of the plant extract of Mycoplasma gallisepticum in the preparation for treating Mycoplasma gallisepticum includes: The plant extract of Mycoplasma gallisepticum and water are mixed in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; Atomize and spray the preventive Mycoplasma gallisepticum mixture on the chicken's head at a dose of 0.1 ml per chicken, twice a day, for 3 to 5 consecutive days.

[0021] In the above scheme, specifically, when spraying, the doors and windows of the chicken house and the exhaust system must be closed before spraying can be carried out. After spraying, the doors and windows and the exhaust system must continue to be closed for 30 minutes to allow the droplets to be suspended in the air of the poultry house. The poultry house can be ventilated after 30 minutes.

[0022] The plant extract of Mycoplasma gallisepticum is formulated to adjust the concentration, and then the formulated liquid medicine is used to perform atomization spray treatment on the chicken's head, so that the liquid medicine can effectively enter the respiratory tract of the chickens, effectively contact and kill the pathogens in the respiratory tract, thereby achieving effective therapeutic effects.

[0023] Preferably, the plant extract of Mycoplasma gallisepticum is used in a preparation for preventing Mycoplasma gallisepticum, and the diameter of the spray is 0.1-5 μm.

[0024] In the above scheme, by limiting the spray diameter, the tiny particles of the plant extract can be better distributed on the respiratory tract and air sacs of the chicken, thereby more effectively exerting its preventive effect.

[0025] Preferably, the use of the plant extract of Mycoplasma gallisepticum in a preparation for treating Mycoplasma gallisepticum includes: when the chickens show upper respiratory tract infection, using an atomizing nozzle with a droplet diameter of 5 μm for spraying; when the chickens show lower respiratory tract infection, using an atomizing nozzle with a droplet diameter of 1 μm for spraying; when the chickens show air sac infection, using an atomizing nozzle with a droplet diameter of 0.1 μm for spraying.

[0026] In the above scheme, specifically, an atomizing nozzle with a droplet diameter of 5 μm is selected for spraying, and larger droplets can be deposited on the mucous membrane of the upper respiratory tract and fully contact with the effective ingredients in the plant extract, thereby exerting a targeted therapeutic effect; an atomizing nozzle with a droplet diameter of 1 μm is selected for spraying, and smaller droplets can penetrate into the lower respiratory tract, including the trachea, bronchi and alveoli, and directly contact with the infected site, thereby improving the therapeutic effect of chickens with lower respiratory tract infections; an atomizing nozzle with a droplet diameter of 0.1 μm is selected for spraying, and extremely small droplets can penetrate into the air sac and fully contact with the infected air sac tissue, thereby effectively inhibiting the growth and reproduction of Mycoplasma gallisepticum and achieving a therapeutic effect.

[0027] Preferably, the spray is sprayed at a height of 30 to 50 cm above the chicken's head.

[0028] In the above scheme, by limiting the spraying height, it can be ensured that the droplets can evenly cover the head, neck and upper body of the chicken, especially near the entrance of the respiratory tract, thereby increasing the contact opportunity between the drug solution and the respiratory mucosa; at the same time, when spraying at this height, the droplets can form a certain diffusion range in the air, thereby being more evenly distributed in the chicken house, improving the spraying efficiency; secondly, spraying at this height can reduce direct stimulation to the chicken and reduce the stress response that may be caused during the spraying process.

[0029] Preferably, for chickens raised in cages, the direction of the spray is limited to a longitudinal fan-shaped air mass during spraying; for chickens raised on a flat surface, the direction of the spray is limited to a transverse fan-shaped air mass during spraying.

[0030] In the above scheme, the direction of the spray output is adjusted according to different feeding environment conditions, so as to better ensure that the liquid medicine can evenly cover each chicken, avoid spraying or repeated spraying, and improve the spray efficiency; at the same time, reduce direct stimulation to the chickens and reduce the stress response that may be caused during the spraying process.

[0031] Preferably, a fan-shaped sprayer is used for spraying, and the fan-shaped sprayer comprises: a liquid medicine tank, a liquid spray pump, a liquid spray pipe, a nozzle assembly and a controller.

[0032] The medicine liquid box has a space for accommodating medicine liquid; a liquid inlet is opened on the upper part of the medicine liquid box, and a cover body is matched with the liquid inlet.

[0033] The liquid spray pump is arranged at the bottom of the liquid medicine tank.

[0034] The liquid spraying pipe is connected to the output end of the liquid spraying pump.

[0035] The nozzle assembly includes a guide plate and an atomizing nozzle, the guide plate has an arc-shaped cavity, the liquid inlet of the guide plate is connected to the liquid spray pipe, a plurality of spray holes are arranged on the side of the guide plate facing outward, the spray holes include three specifications with diameters of 0.1 μm, 1 μm and 5 μm, and the three specifications of spray holes are evenly distributed on one side of the guide plate; the atomizing nozzle includes three specifications of atomizing nozzles with diameters of 0.1 μm, 1 μm and 5 μm, and the three specifications of atomizing nozzles are respectively connected to the spray holes of corresponding specifications.

[0036] A controller is connected with the liquid spray pump.

[0037] In the above scheme, the medicine liquid tank is used to store the mixed liquid for preventing or treating Mycoplasma gallisepticum; a liquid inlet is provided at the upper part of the medicine liquid tank for convenient addition of medicine liquid; the liquid inlet is equipped with a cover to keep the medicine liquid clean and prevent volatilization. The liquid medicine is pumped out of the medicine liquid tank and pressurized to the liquid spray pipe by the power provided by the liquid spray pump; the liquid spray pump is arranged at the bottom of the liquid medicine tank to ensure that the liquid medicine can be completely pumped out; the liquid medicine is then transported to the guide plate through the liquid spray pipe, and the shape of the spray is limited to a fan-shaped distribution through the structural restriction of the guide plate, and the liquid medicine spray is further refined through the atomizing nozzle to improve the spray effect.

[0038] Advantages of the present invention: First, the plant extract of Mycoplasma gallisepticum of the present invention has a synergistic effect on inhibiting or killing Mycoplasma gallisepticum by mixing the raw materials.

[0039] Second, the preparation method of the plant extract of Mycoplasma gallisepticum of the present invention is to first grind the raw material and then add boiling water to stir it into a paste, which helps the effective ingredients in the raw material to be better dissolved in water; then freeze-thaw treatment helps to change the molecular structure of the raw material and improve the subsequent extraction efficiency; and then mix it with acetic acid in proportion to further enhance the antibacterial activity of the extract.

[0040] Third, the plant extract of Mycoplasma gallisepticum of the present invention is used in a preparation for preventing Mycoplasma gallisepticum. The plant extract of Mycoplasma gallisepticum is formulated to adjust the concentration, and then the formulated liquid medicine is used to perform atomization spray treatment on the head of the chicken, so that the liquid medicine can effectively enter the upper respiratory tract of the chickens, thereby forming a protective film on the respiratory mucosa to prevent infection with Mycoplasma gallisepticum.

[0041] Fourth, the plant extract of Mycoplasma gallisepticum of the present invention is used in a preparation for treating Mycoplasma gallisepticum. The plant extract of Mycoplasma gallisepticum is formulated to adjust the concentration, and then the formulated liquid medicine is used to perform atomization spray treatment on the head of the chicken, so that the liquid medicine can effectively enter the respiratory tract of the chickens, effectively contact and kill the pathogens in the respiratory tract, thereby achieving an effective therapeutic effect.

[0042] Fifth, the use of the plant extract of Mycoplasma gallisepticum of the present invention in a preparation for preventing Mycoplasma gallisepticum can help the tiny particles of the plant extract to be better distributed in the respiratory tract and air sacs of the chicken by limiting the spray diameter, thereby more effectively exerting its preventive effect.

[0043] Sixth, the use of the plant extract of Mycoplasma gallisepticum of the present invention in a preparation for treating Mycoplasma gallisepticum, using an atomizing nozzle with a droplet diameter of 5 μm for spraying, larger droplets can be deposited on the mucous membrane of the upper respiratory tract, fully contact with the effective ingredients in the plant extract, thereby playing a targeted therapeutic effect; using an atomizing nozzle with a droplet diameter of 1 μm for spraying, smaller droplets can penetrate into the lower respiratory tract, including the trachea, bronchi and alveoli, directly contact with the infected site, and improve the therapeutic effect of chickens infected with the lower respiratory tract; using an atomizing nozzle with a droplet diameter of 0.1 μm for spraying, extremely small droplets can penetrate into the air sac, fully contact with the infected air sac tissue, thereby effectively inhibiting the growth and reproduction of Mycoplasma gallisepticum, and achieving a therapeutic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 A schematic diagram of the structure of the fan-shaped sprayer of the present invention; Figure 2 It is a schematic diagram of the structure of the guide plate in the fan-shaped sprayer of the present invention; In the figure, there are a liquid medicine tank 1, a liquid spray pipe 2, a nozzle assembly 3, a guide plate 31, and an atomizing nozzle 32. DETAILED DESCRIPTION

[0045] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments so that those skilled in the art can implement the invention with reference to the description.

[0046] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0047] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0048] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense. For example, they can be fixedly connected or set, or detachably connected or set, or connected or set in one piece. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The orientations or positional relationships indicated by the terms "lateral", "longitudinal", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0049] The plant extract of Mycoplasma gallisepticum comprises the following raw materials obtained in the following weight parts respectively: 45-50 parts of pine needles, 30-40 parts of licorice, 30-40 parts of coptis root, 25-35 parts of phellodendron, 20-30 parts of bupleurum, 17-25 parts of dandelion, 15-20 parts of desmodium, 7-15 parts of plantain and 5-10 parts of tuckahoe.

[0050] A method for preparing a plant extract of Mycoplasma gallisepticum comprises: The following raw materials are obtained according to the following weight portions: 45-50 parts of pine needles, 30-40 parts of licorice, 30-40 parts of coptis chinensis, 25-35 parts of phellodendron, 20-30 parts of bupleurum, 17-25 parts of dandelion, 15-20 parts of moneywort, 7-15 parts of plantain, and 5-10 parts of poria.

[0051] The raw materials are crushed and then added into boiling water and stirred into a paste to obtain a paste.

[0052] The paste was frozen at -25°C for 24 hours and then thawed at room temperature to obtain a mixture.

[0053] The mixture was mixed with 95% ethanol solution in a mass ratio of 1:3, and organic extraction and vacuum concentration were performed in sequence at room temperature to obtain a clear paste with a density of 1.18-1.25.

[0054] The clear paste was mixed with 20% acetic acid in a mass ratio of 1:3 to obtain the plant extract of Mycoplasma gallisepticum.

[0055] like Figure 1~2 As shown, the fan-shaped sprayer includes: a liquid tank 1, a liquid spray pump, a liquid spray pipe 2, a nozzle assembly 3 and a controller.

[0056] The medicine liquid box 1 has a space for accommodating medicine liquid; a liquid inlet is opened on the upper part of the medicine liquid box 1, and a cover body is provided in conjunction with the liquid inlet.

[0057] The liquid spray pump is arranged at the bottom of the liquid medicine tank 1 .

[0058] The liquid spraying pipe 2 is connected to the output end of the liquid spraying pump.

[0059] The nozzle assembly 3 includes a guide plate 31 and an atomizing nozzle 32. The guide plate 31 has an arc-shaped cavity. The liquid inlet of the guide plate 31 is connected to the liquid spray pipe 2. A plurality of spray holes are arranged on the side of the guide plate 31 facing outward. The spray holes include three specifications with diameters of 0.1 μm, 1 μm and 5 μm. The three specifications of spray holes are evenly distributed on one side of the guide plate 31; the atomizing nozzle 32 includes three specifications with diameters of 0.1 μm, 1 μm and 5 μm. The three specifications of atomizing nozzles 32 are respectively connected to the spray holes of corresponding specifications.

[0060] A controller is connected with the liquid spray pump.

[0061] Example 1 Take 45 parts of pine needles, 30 parts of licorice, 30 parts of coptis root, 25 parts of phellodendron, 20 parts of bupleurum, 17 parts of dandelion, 15 parts of money grass, 7 parts of plantain, and 5 parts of poria respectively; grind all the raw materials and add boiling water to stir into a paste to obtain a paste; freeze the paste at -25°C for 24 hours, and thaw it at room temperature to obtain a mixture; mix the mixture with 95% ethanol solution in a volume ratio of 1:3, extract at room temperature for 3 hours, repeat the filtration at least 3 times, combine the filtrate and evaporate the ethanol by vacuum distillation, and then place it in a vacuum rotary instrument under a constant temperature of 60°C in a water bath to concentrate it to a relative density of 1.18~1.25; mix the clear paste with 20% acetic acid in a mass ratio of 1:3 to obtain the plant extract of Mycoplasma gallisepticum. Among them, the particle size of the raw materials after grinding is 50 mesh; except for the freezing link, the environment of other working links is 18~30°C and 30%~70% relative humidity.

[0062] Example 2 Take 50 parts of pine needles, 40 parts of licorice, 40 parts of coptis root, 35 parts of phellodendron, 30 parts of bupleurum, 25 parts of dandelion, 20 parts of money grass, 15 parts of plantain, and 10 parts of poria respectively; grind all the raw materials and add boiling water to stir into a paste to obtain a paste; freeze the paste at -25°C for 24 hours, and thaw it at room temperature to obtain a mixture; mix the mixture with 95% ethanol solution in a volume ratio of 1:3, extract at room temperature for 3 hours, repeat the filtration at least 3 times, combine the filtrate and evaporate the ethanol by vacuum distillation, and then place it in a vacuum rotary instrument under a constant temperature of 60°C in a water bath to concentrate it to a relative density of 1.18~1.25; mix the clear paste with 20% acetic acid in a mass ratio of 1:3 to obtain the plant extract of Mycoplasma gallisepticum. Among them, the particle size of the raw materials after grinding is 50 mesh; except for the freezing link, the environment of other working links is a temperature of 18~30°C and a relative humidity of 30%~70%.

[0063] Test and analysis Test 1 The test site is located in the three virus-challenged isolation chicken houses of the Poultry Research Institute of the Chinese Academy of Agricultural Sciences located in the Poultry Research and Breeding Base in Shaobo Town, Jiangdu District, Yangzhou City; the test subjects are 93-day-old young Hy-Line laying hens. The test time is from March 29, 2024 to April 10, 2024; 700 93-day-old young Hy-Line laying hens of the same batch were selected for artificial virus-challenged experiments, and the virus-challenged type is Mycoplasma gallisepticum that infects all parts of the chicken respiratory system. After the virus-challenged, they were divided into 7 groups on average, with 100 chickens in each group, and were raised in 3 virus-challenged isolation chicken houses, and the chickens were raised on a flat surface. After the test chickens showed symptoms of Mycoplasma gallisepticum, the plant extracts of Mycoplasma gallisepticum prepared in Examples 1 and 2 were mixed with water in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; the mixed solution for preventing Mycoplasma gallisepticum was atomized and sprayed on the head of the chicken at a dose of 0.1 ml per chicken, sprayed twice a day, and used continuously for 5 days; during atomization, three atomizing nozzles with diameters of 0.1 μm, 1 μm and 5 μm were used to perform atomization and spraying operations simultaneously; the spray height was 35 to 45 cm; and the spray shape was a horizontal fan-shaped air mass. Test groups 3 to 5 all used the plant extract of Mycoplasma gallisepticum prepared in Example 1 and water in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; the mixed solution for preventing Mycoplasma gallisepticum was atomized and sprayed on the head of the chicken at a dose of 0.1 ml per chicken, sprayed twice a day, and used continuously for 5 days; among them, in the experimental group 3, an atomizing nozzle with a diameter of 0.1 μm was used for atomization and spraying at the same time; the spray height was 35 to 45 cm; and the spray shape was a horizontal fan-shaped air mass. In the test group 4, an atomizing nozzle with a diameter of 1 μm was used for atomization and spraying at the same time; the spray height was 35 to 45 cm; and the spray shape was a horizontal fan-shaped air mass. In the experimental group 5, an atomizing nozzle with a diameter of 5 μm was used for atomization and spraying at the same time; the spray height was 35 to 45 cm; and the spray shape was a horizontal fan-shaped air mass. The control group was treated with a mixed solution containing tylosin, tylosin, doxycycline and azithromycin according to the drug instructions, and the blank group was not treated. After the treatment of all groups of chickens was completed, the chickens were observed for another week and the survival of each group was recorded. The treatment cost was the total cost of each group of drugs during the trial / the initial number of chickens in the trial; the results are shown in Table 1.

[0064] Table 1: Survival of chickens in each group Test 2 The test time is from March 22, 2024 to April 24, 2024; the test site is at the laying hen farm of Taizhou Agricultural Technology Co., Ltd. in Heheng Village, Shengao Town, Jiangyan District, Taizhou City, Jiangsu Province; the test subjects are chickens infected with Mycoplasma gallisepticum in 2 chicken houses, and the chickens are caged. Before the test, the dead chickens were autopsied, and the upper respiratory tract, lower respiratory tract, bronchi, lungs, and air sacs of the dead chickens showed obvious symptoms of Mycoplasma gallisepticum lesions. Among them, the chickens in this chicken house have been treated with tylosin and azithromycin for drinking water after the onset of the disease, but they have been recurring.

[0065] The plant extract of Mycoplasma gallisepticum prepared in Example 1 and water were mixed in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; the mixed solution for preventing Mycoplasma gallisepticum was atomized and sprayed on the head of the chicken at a dose of 0.1 ml per chicken, sprayed twice a day, and used continuously for 5 days; during atomization, atomizing nozzles with three diameter specifications of 0.1 μm, 1 μm and 5 μm were simultaneously atomized and sprayed; the spray height was 30-35 cm; the spray shape was a longitudinal fan-shaped air mass. After the treatment was completed, the chickens were observed for 4 weeks, and the survival of the chickens from the first week to the fourth week was recorded and recorded, and the results are shown in Table 2.

[0066] Table 2: Survival of chicken flocks Test 3 The test time is from February 19, 2024 to March 23, 2024; the test site is a broiler farm in Gaoyou City, Ergou, Gaoyou City, Yangzhou City, Jiangsu Province; the experimental subject is a broiler house infected with Mycoplasma gallisepticum, and the chickens are raised on a flat surface. Before the test, the dead chickens were autopsied, and the upper respiratory tract, lower respiratory tract, bronchi, lungs, and air sacs of the dead chickens showed obvious symptoms of Mycoplasma gallisepticum lesions. Among them, the chickens in this chicken house have been treated with tylosin, doxycycline, and tylosin after the onset of the disease, but they have been recurring. The plant extract of Mycoplasma gallisepticum prepared in Example 1 and water were mixed in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; the mixed solution for preventing Mycoplasma gallisepticum was atomized and sprayed on the head of the chicken at a dose of 0.1 ml per chicken, sprayed twice a day, and used continuously for 5 days; during atomization, atomizing nozzles with three diameter specifications of 0.1 μm, 1 μm and 5 μm were simultaneously atomized and sprayed; the spray height was 45 to 50 cm; the spray shape was a horizontal fan-shaped air mass. After the treatment was completed, the chickens were observed for 4 weeks, and the survival of the chickens from the first week to the fourth week was recorded and recorded, and the results are shown in Table 3.

[0067] Table 3: Survival of chicken flocks Test 4 The test was conducted from January 19, 2024 to February 21, 2024 at a chicken farm in Ganquan Village, Ganduo Town, Gaoyou City, Yangzhou City, Jiangsu Province. Due to the cold and wet climate in Jiangsu Province in winter, the farm did not take proper measures to prevent cold and keep warm, and all free-range broiler houses were infected with Mycoplasma gallisepticum. Before the test, the dead chickens were autopsied, and most of the respiratory systems of the dead chickens had obvious symptoms of Mycoplasma gallisepticum. After the chickens became ill, they were treated with tylosin, doxycycline, tylosin, and azithromycin, but the disease kept recurring.

[0068] The plant extract of Mycoplasma gallisepticum prepared in Example 1 and water were mixed in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; the mixed solution for preventing Mycoplasma gallisepticum was atomized and sprayed on the head of the chicken at a dose of 0.1 ml per chicken, sprayed twice a day, and used continuously for 5 days; during atomization, atomizing nozzles with three diameter specifications of 0.1 μm, 1 μm and 5 μm were simultaneously atomized and sprayed; the spray height was 40-45 cm; the spray shape was a horizontal fan-shaped air mass. After the treatment was completed, the chickens were observed for 4 weeks, and the survival of the chickens from the first week to the fourth week was recorded and recorded, and the results are shown in Table 4.

[0069] Table 4: Survival of chicken flocks Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and the embodiments. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.

Claims

1. A plant extract of Mycoplasma gallisepticum, characterized in that The following raw materials are obtained in the following weight parts: 45-50 parts of pine needles, 30-40 parts of liquorice, 30-40 parts of coptis root, 25-35 parts of phellodendron, 20-30 parts of bupleurum, 17-25 parts of dandelions, 15-20 parts of moneywort, 7-15 parts of plantain, and 5-10 parts of poria.

2. The method for preparing the plant extract of Mycoplasma gallisepticum according to claim 1, characterized in that: include: The following raw materials are obtained according to the following weight portions: 45-50 parts of pine needles, 30-40 parts of licorice, 30-40 parts of coptis root, 25-35 parts of phellodendron, 20-30 parts of bupleurum, 17-25 parts of dandelion, 15-20 parts of desmodium, 7-15 parts of plantain, and 5-10 parts of tuckahoe; The raw materials are crushed and then added with boiling water and stirred into a paste to obtain a paste; The paste is frozen at a temperature of -25°C for 24 hours and then thawed at room temperature to obtain a mixture; The mixture was mixed with 95% ethanol solution in a mass ratio of 1:3, and organic extraction and vacuum concentration were performed in sequence at room temperature to obtain a clear paste with a density of 1.18-1.25; The clear paste was mixed with 20% acetic acid in a mass ratio of 1:3 to obtain the plant extract of Mycoplasma gallisepticum.

3. Use of the plant extract of Mycoplasma gallisepticum as claimed in claim 1 in a preparation for preventing and treating Mycoplasma gallisepticum.

4. The use according to claim 3, characterized in that include: The plant extract of Mycoplasma gallisepticum and water are mixed in a volume ratio of 1:5 to obtain a mixed solution for preventing Mycoplasma gallisepticum; Atomize and spray the chicken's head with a mixed solution for preventing Mycoplasma gallisepticum at a dose of 0.1 ml per chicken, twice a week.

5. The use according to claim 3, characterized in that include: The plant extract of Mycoplasma gallisepticum and water are mixed in a volume ratio of 1:3 to obtain a mixed solution for treating Mycoplasma gallisepticum; Atomize and spray the preventive Mycoplasma gallisepticum mixture on the chicken's head at a dose of 0.1 ml per chicken, twice a day, for 3 to 5 consecutive days.

6. The use according to claim 4, characterized in that The diameter of the spray is 0.1~5μm.

7. The use according to claim 5, characterized in that When the chickens show signs of upper respiratory tract infection, use an atomizer nozzle with a droplet diameter of 5μm for spraying; when the chickens show signs of lower respiratory tract infection, use an atomizer nozzle with a droplet diameter of 1μm for spraying; when the chickens show signs of air sac infection, use an atomizer nozzle with a droplet diameter of 0.1μm for spraying.

8. The use according to any one of claims 4 or 5, characterized in that: The spray is sprayed at a height of 30 to 50 cm above the chicken's head.

9. The use according to any one of claims 4 or 5, characterized in that: For chickens raised in cages, the direction of the spray is limited to the longitudinal fan-shaped air mass; for chickens raised on a flat surface, the direction of the spray is limited to the transverse fan-shaped air mass.

10. The use according to any one of claims 4 or 5, characterized in that: A fan-shaped sprayer is used for spraying, and the fan-shaped sprayer includes: a liquid medicine tank, a liquid spray pump, a liquid spray pipe, a nozzle assembly and a controller; A liquid medicine box having a space for containing liquid medicine; a liquid inlet is provided on the upper portion of the liquid medicine box, and a cover body is provided in cooperation with the liquid inlet; A liquid spray pump, which is arranged at the bottom of the liquid medicine tank; A liquid spray pipe connected to the output end of the liquid spray pump; A nozzle assembly, comprising a guide plate and an atomizing nozzle, wherein the guide plate has an arc-shaped cavity, the liquid inlet of the guide plate is connected to the liquid spray pipe, a plurality of spray holes are arranged on one side of the guide plate facing outward, the spray holes include three specifications of diameters of 0.1 μm, 1 μm and 5 μm, and the three specifications of the spray holes are evenly distributed on one side of the guide plate; the atomizing nozzle includes three specifications of atomizing nozzles with diameters of 0.1 μm, 1 μm and 5 μm, and the three specifications of the atomizing nozzles are respectively connected to the spray holes of corresponding specifications; A controller is connected with the liquid spray pump.

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