Folium artemisiae argyi composition for regulating functions of internal organs as well as preparation and application of folium artemisiae argyi composition

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a folium artemisiae argyi composition for regulating viscera functions as well as preparation and application of the folium artemisiae argyi composition. The method comprises the following steps: S1, mixing folium artemisiae argyi, radix salviae miltiorrhizae, radix bupleuri, rhizoma atractylodis macrocephalae, radix platycodonis, radix rehmanniae praeparata, pericarpium citri reticulatae and water, and performing enzymolysis to obtain a solid-liquid mixture; s2, mixing the solid-liquid mixture with a culture medium, inoculating rhizopus chinensis, and performing primary fermentation; s3, after inactivation, bacillus subtilis is inoculated for secondary fermentation, and a liquid-phase substance is obtained; s4, filtering and concentrating the liquid-phase substance to obtain a traditional Chinese medicine extract; s5, mixing the traditional Chinese medicine extract with the fruit and vegetable enzymolysis extract to obtain a folium artemisiae argyi composition; the fruits and vegetables in the fruit and vegetable enzymolysis extract comprise lily, spinach, broccoli and celery. According to the invention, a plurality of traditional Chinese medicine extracts and fruit and vegetable enzymolysis extracts are compounded to synergistically improve the function of regulating the viscera, and a microbial fermentation technology is utilized to eliminate toxic components of a plurality of traditional Chinese medicines, so that the function of regulating the viscera is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an wormwood leaf composition for regulating viscera functions and a preparation method and application thereof. Background Art

[0002] Moxibustion is a traditional Chinese medicine that uses mugwort as the main ingredient. After being picked, the mugwort is repeatedly dried, beaten, and sieved to remove impurities, and then made into soft moxa wool. Moxa cones are made of moxa wool in a cone shape of varying sizes, and are used for direct or indirect moxibustion. Moxa sticks are moxa wool wrapped in cotton paper and rolled into a cylindrical shape, which is convenient for hand-held moxibustion. Moxibustion has a unique effect on regulating the functions of internal organs, especially the lungs, spleen, and kidneys. However, the function of moxibustion alone in regulating the lungs is limited, and moxa leaves cannot be taken directly orally to have an effect on the human body, and are even toxic.

[0003] For example, CN105412192A discloses an anti-mite cream from mugwort and an extraction method for mugwort essential oil, and the raw materials include mugwort essential oil, jojoba oil, soybean lecithin, vitamin E acetate, glycerol, polyacrylamide and deionized water. The anti-mite cream from mugwort provided in the application is made by a thermal process, and the anti-mite cream contains mugwort essential oil, which is obtained by supercritical CO 2 The extract is prepared by the method of extraction and rotary evaporation, has the effect of inhibiting bacteria and killing mites, and retains the original aroma of mugwort without any added essence. However, the application only utilizes the effect of volatile oil and does not have the function of regulating internal organs.

[0004] For example, CN114533833A discloses a Chinese medicine composition for warming and nourishing the internal organs, which includes, by weight: 8-25 parts of Atractylodes, 5-15 parts of Atractylodes macrocephala, 5-15 parts of Rhizoma Coptidis, 1-10 parts of Coptis chinensis, 5-17 parts of Rhizoma Cimicifugae, 1-10 parts of Rhizoma Cimicifugae, 1-10 parts of Cinnamon bark, 1-8 parts of Zanthoxylum bungeanum, 5-16 parts of Kaempferia galanga, 5-15 parts of Angelica dahurica, 1-8 parts of Amomum villosum, 1-8 parts of Rhizoma Cibotii, 1-8 parts of Rhizoma Cibotii, 5-15 parts of Amomum villosum, 0.5-5 parts of Pseudostellariae, 1-8 parts of Codonopsis pilosula, 0.5-5 parts of Dioscorea opposita, and 0.1-1 parts of Borneol. The Chinese medicine composition for warming and nourishing the internal organs provided in the application can be used externally, and within a certain ratio range, it can effectively improve the symptoms of night crying, anger and abdominal distension, vomiting and diarrhea in newborns caused by internal organ dysfunction, and has a significant therapeutic effect on abdominal pain and hepatosplenomegaly in school-age children. However, this application is only an ordinary method of preparing Chinese medicine, which cannot bring out the effectiveness of mugwort leaves, and the toxic components of various Chinese medicines cannot be removed, so their effectiveness cannot be effectively exerted.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The purpose of the present invention is to provide an wormwood leaf composition for regulating the functions of internal organs, and its preparation and application. The present invention compounds a variety of traditional Chinese medicine extracts and fruit and vegetable enzymatic extracts to synergistically improve the functions of regulating internal organs, and utilizes microbial fermentation technology to eliminate the toxic components of a variety of traditional Chinese medicines, thereby improving the functions of regulating internal organs.

[0007] In order to achieve the above object, the present invention provides the following technical solutions: In a first aspect, an embodiment of the present invention provides a method for preparing a wormwood composition for regulating the functions of internal organs, comprising the following steps: S1, mixing wormwood leaf, salvia miltiorrhiza, bupleurum root, atractylodes macrocephala, platycodon grandiflorum, rehmannia root, tangerine peel and water, and performing enzymolysis to obtain a solid-liquid mixture; S2, mixing the solid-liquid mixture with a culture medium and then inoculating Rhizopus chinensis to perform a fermentation; S3, inoculating Bacillus subtilis after inactivation for secondary fermentation to obtain a liquid phase; S4, filtering the liquid phase and concentrating it to obtain a Chinese medicine extract; S5, mixing the Chinese medicinal extract with the fruit and vegetable enzymatic extract to obtain an Artemisia argyi composition; The fruits and vegetables in the fruit and vegetable enzymatic extract include lily, spinach, broccoli and celery.

[0008] The compounded wormwood leaf of the present invention has excellent regulating function on the internal organs of the human body after being combined with a variety of Chinese herbal medicines, and the substances in the Chinese medicine are completely extracted through enzymatic hydrolysis and two fermentation processes, and the two fermentations further remove the toxic components of the Chinese medicine. In addition, the present invention mixes a variety of fruit and vegetable enzymatic extracts in the Chinese medicine extract, and the fruit and vegetable enzymatic extracts can further remove the toxic components in the Chinese medicine, improve the taste of the obtained preparation, and the fruit and vegetable enzymatic extracts and the Chinese medicine composition synergistically improve the function of regulating the internal organs.

[0009] Specifically, in terms of liver regulation, Danshen has the effects of promoting blood circulation and removing blood stasis, relieving pain through menstruation, and clearing the heart and removing vexation. Its blood circulation effect helps improve the blood circulation of the liver. Bupleurum can reconcile the exterior and interior, relieve liver depression, and make the liver qi smooth and unobstructed. The two work together, Danshen promotes blood circulation, and Bupleurum combs liver qi, and they can jointly play a regulatory role in the liver qi and blood stagnation caused by liver depression and blood stasis, such as flank pain, irregular menstruation, and other liver qi and blood stagnation. And Atractylodes can strengthen the spleen and replenish qi, dry dampness and promote diuresis, and indirectly regulate the liver by enhancing spleen function. Because in the theory of the five elements of traditional Chinese medicine, the spleen belongs to earth and the liver belongs to wood, and earth can cultivate wood, and strengthening the spleen helps prevent liver wood from being too hyperactive. Tangerine peel regulates qi and strengthens the spleen, dries dampness and resolves phlegm, and its qi-regulating effect can help regulate the liver qi. When the liver is affected by pathogenic factors, liver depression invades the spleen (i.e., liver qi invades the spleen), leading to abnormal spleen and stomach transport and transformation, white atractylodes and tangerine peel are used together. On the one hand, white atractylodes enhances the spleen and stomach function, and on the other hand, tangerine peel regulates the qi mechanism to prevent liver qi from further invading the spleen and stomach, thereby maintaining the normal relationship between the liver and the spleen and stomach, and achieving the purpose of coordinated regulation of liver function. In addition, Rehmannia glutinosa nourishes yin and blood, replenishes essence and fills the marrow, and has a good tonic effect on liver yin deficiency. Bupleurum soothes the liver and relieves depression. When regulating the liver, Bupleurum glutinosa can better transport the tonic ingredients of Rehmannia glutinosa to the liver meridians, allowing Rehmannia glutinosa to play a role in nourishing liver yin. At the same time, Bupleurum glutinosa prevents tonic drugs from producing greasiness and stagnation, leading to liver depression. In the case of liver yin deficiency and mild liver depression, Rehmannia glutinosa nourishes liver yin, and Bupleurum soothes liver qi, jointly improving the liver's yin and yang, qi and blood balance. Artemisia argyi is warm in nature, pungent and bitter in taste, and belongs to the liver, spleen, and kidney meridians. Its warm nature can provide a warm environment for the liver and promote the circulation of qi and blood. When evil cold stagnates in the liver meridian, such as cold stagnation in the liver meridian, it can cause symptoms such as cold pain in the lower abdomen and hernia pain. Mugwort can dispel the evil cold and restore the smooth flow of qi and blood in the liver meridian.

[0010] In terms of heart regulation, Danshen can promote blood circulation, remove blood stasis, relieve pain, and play an important role in improving the blood circulation of the heart and preventing blood stasis in the heart. Platycodon grandiflorum can promote lung function, relieve sore throat, remove phlegm, and discharge pus. At the same time, Platycodon grandiflorum can also carry medicine upward. When regulating the heart, Platycodon grandiflorum can help the medicinal power of drugs such as Danshen to better act on the heart. In the treatment of chest pain, Danshen can dissolve blood stasis, and Platycodon grandiflorum can promote lung qi. Because the heart and lungs coexist in the upper part of the body, the smooth flow of lung qi helps the circulation of heart blood. The two can work together to relieve symptoms such as chest pain and chest tightness caused by blood stasis in the heart. Rehmannia root nourishes yin and replenishes blood, and has a good tonic effect on the heart deficiency caused by insufficient heart blood. Artemisia argyi warms the meridians and stops bleeding, dispels cold and relieves pain. When the heart yang qi is insufficient and there are cold palpitations and palpitations, the warming yang effect of artemisia argyi is combined with the nourishing yin and blood effect of Rehmannia root, and yin and yang are adjusted, which can not only replenish heart blood, but also invigorate heart yang and improve heart function. For patients with both heart yang deficiency and heart blood deficiency, mugwort warms the heart yang and rehmannia replenishes the heart blood, and together they regulate the balance of yin and yang, qi and blood in the heart.

[0011] In terms of spleen regulation, Atractylodes macrocephala strengthens the spleen and replenishes qi, and enhances the spleen's transportation and transformation function. Platycodon grandiflorum opens and clears the lung qi. According to the theory of traditional Chinese medicine, the lung governs the qi of the whole body, and the smooth flow of lung qi helps the spleen qi to ascend. The two work together. Platycodon grandiflorum indirectly promotes the transportation and transformation of the spleen by regulating lung qi, while Atractylodes macrocephala directly enhances the function of the spleen, which has a good regulating effect on conditions such as spleen and stomach weakness, inability to transport and transform, and loss of appetite. When the transportation and transformation are abnormal due to spleen qi deficiency, and symptoms such as fullness in the stomach and loose stools appear, Atractylodes macrocephala enhances the spleen and stomach function, and Platycodon grandiflorum regulates lung qi and promotes the transportation and transformation of the spleen and stomach, so that the symptoms are improved. In addition, tangerine peel regulates qi and strengthens the spleen, and can eliminate spleen and stomach qi stagnation. Bupleurum soothes the liver and relieves depression. Since there is a restraining relationship between liver wood and spleen earth, the smooth flow of liver qi helps the normal transportation and transformation of the spleen and stomach. When the liver is depressed and the spleen is deficient, tangerine peel and bupleurum work together. Bupleurum soothes liver depression and prevents liver qi from attacking the spleen, and tangerine peel regulates the spleen and stomach qi, and together restores the normal function of the spleen and stomach. In the case of indigestion caused by liver depression and spleen deficiency due to poor mood, Bupleurum regulates mood and combs liver qi, while Tangerine peel improves spleen and stomach qi stagnation, and together relieves symptoms such as abdominal distension and belching. Artemisia argyi is pungent and bitter in taste, warm in nature, and belongs to the spleen meridian. Its warm nature can warm and replenish spleen yang. Just like in a cold environment, the land (analogous to the spleen) will become stiff and difficult to cultivate (analogous to abnormal transportation and transformation), while sunlight (analogous to the warming yang effect of artemisia argyi) can warm up the land and restore vitality. When spleen yang is insufficient, there will be a situation where transportation and transformation are weak and the essence of water and grain cannot be properly distributed, such as abdominal distension, loose stools, and loss of appetite. Artemisia argyi can provide warm energy for the spleen and enhance the spleen's transportation and transformation function.

[0012] In terms of lung regulation, Platycodon grandiflorum can clear the lungs and eliminate phlegm, and has a good effect on relieving symptoms such as cough, sputum, and chest tightness caused by stagnation of lung qi. Mugwort warms the meridians and dispels cold. In the case of lung cold cough, the warm nature of mugwort can dispel lung cold, and platycodon grandiflorum helps the lung qi to be smooth, making it easier to cough up phlegm. In the case of wind-cold cough caused by cold evil, mugwort warms the lungs and dispels cold, and platycodon grandiflorum clears the lungs and relieves cough. The two work together to improve lung function and relieve cough symptoms. In addition, bupleurum soothes the liver and relieves depression, and platycodon grandiflorum clears the lungs and relieves throat. In traditional Chinese medicine theory, there is a restraining relationship between liver wood and lung metal, and lung function can be indirectly affected by regulating the liver qi. Bupleurum grandiflorum regulates liver qi, and platycodon grandiflorum clears lung qi. The two work together to regulate the qi balance between the liver and lungs. In the case of cough and hemoptysis caused by liver depression turning into fire and going up against the lungs (similar to the liver fire invading the lungs syndrome), bupleurum clears the liver and purges fire, soothes the liver and regulates qi, and platycodon grandiflorum clears the lungs and eliminates phlegm, stops cough and stops bleeding, and they work together to regulate liver and lung function and relieve symptoms.

[0013] In terms of kidney regulation, Rehmannia glutinosa can nourish yin and blood, replenish essence and fill marrow, and has a good tonic effect on kidney yin deficiency. Atractylodes macrocephala can strengthen the spleen and replenish qi. In traditional Chinese medicine theory, the spleen and kidney are closely related. The kidney is the foundation of the innate, the spleen is the foundation of the acquired, and the acquired nourishes the innate. Atractylodes macrocephala can provide more nutrients to the kidneys and promote the metabolization of kidney essence by enhancing the spleen and stomach function. For patients with kidney yin deficiency accompanied by weak spleen and stomach, Rehmannia glutinosa nourishes kidney yin, and Atractylodes macrocephala strengthens the spleen and helps transport. The two synergistically enhance the tonic effect on the kidney and improve the symptoms of kidney yin deficiency. In addition, Salvia miltiorrhiza promotes blood circulation and removes blood stasis, while Rehmannia glutinosa nourishes yin and nourishes the kidney. In the case of poor blood circulation in the kidneys, blood stasis, and kidney yin deficiency, Salvia miltiorrhiza can improve kidney blood circulation, and Rehmannia glutinosa nourishes kidney yin. When chronic kidney disease patients have a pathological state of kidney yin deficiency and blood stasis, Salvia miltiorrhiza and Rehmannia glutinosa work synergistically. On the one hand, Salvia miltiorrhiza removes blood stasis and improves kidney blood perfusion, and on the other hand, Rehmannia glutinosa replenishes kidney yin and regulates kidney function. What's more, mugwort leaves are warm in nature and belong to the liver, spleen, and kidney meridians. Its warm nature can warm up the kidney yang. Kidney yang is like a small fire in the human body, which can warm up the organs and meridians of the whole body. When kidney yang is insufficient, symptoms such as soreness of waist and knees, chills and cold limbs, and frequent urination at night will appear. Mugwort leaves can provide a certain amount of warming power for kidney yang, just like adding fuel to a small fire, enhancing the function of kidney yang.

[0014] In a preferred embodiment, in step S1, the mass ratio of wormwood leaf, salvia miltiorrhiza, bupleurum root, atractylodes macrocephala, platycodon grandiflorum, rehmannia root, tangerine peel and water is (50-70): (20-30): (8-13): (5-9): (6-13): (7-20): (2-8): (200-400).

[0015] In a preferred embodiment, in step S1, the specific steps of enzymatic hydrolysis are: Add complex enzyme to a mixture of Artemisia argyi, Salvia miltiorrhiza, Bupleurum chinense, Atractylodes macrocephala, Platycodon grandiflorum, Rehmannia glutinosa, Tangerine peel and water, and perform enzymolysis at 30-40°C for 12-18h; The complex enzyme comprises cellulase, amylase and pectinase, and the mass ratio of the cellulase, amylase and pectinase is 2:(0.5-1):(0.5-1).

[0016] In a preferred embodiment, the mass ratio of the wormwood leaf to the complex enzyme is 100:(1-4).

[0017] The invention combines a variety of complex enzymes to effectively improve the extraction rate of effective components in subsequent fermentation treatment, and can appropriately remove toxic components in some Chinese herbal medicines.

[0018] Specifically, the active ingredients of Chinese herbal medicines are usually encapsulated in plant cells or combined with other ingredients. Enzymatic hydrolysis can destroy plant cell walls and release the active ingredients in the cells. Cellulase and pectinase can decompose the cellulose and pectin components of the cell walls, making the structure of the cell walls loose, and the active ingredients originally restricted by the cell walls can be more easily dissolved from the cells into the extraction solvent. In addition, the toxic ingredients contained in Chinese herbal medicines may exist in the form of some macromolecular compounds or combined with other ingredients. During the enzymatic hydrolysis process, the structure of the toxic components will change, converting them into small molecules with low or no toxicity.

[0019] In a preferred embodiment, in step S2, the culture medium is PDA culture medium.

[0020] In a preferred embodiment, in step S2, based on the mass of the solid-liquid mixture of 100 g, the viable count of Rhizopus chinensis is 1×10 10 -2×10 11 CFU.

[0021] In a preferred embodiment, in step S2, the temperature of the primary fermentation is 25-30°C, the time is 24-72h, and the pH is 6.5-7.5.

[0022] In a preferred embodiment, in step S3, based on the mass of the solid-liquid mixture of 100 g, the viable count of the Bacillus subtilis is 1×10 10 -2×10 11 CFU.

[0023] In a preferred embodiment, in step S3, the temperature of the secondary fermentation is 25-30°C, the time is 24-72h, and the pH is 6.5-7.5.

[0024] The present invention not only removes toxic components in Chinese herbal medicines through Rhizopus chinensis and Bacillus subtilis, but also improves the efficacy of the Chinese herbal medicines through the synergy of their metabolites and the Chinese herbal medicines themselves, and at the same time improves the absorption and utilization efficiency of the medicines.

[0025] In terms of removing toxic components, Rhizopus chinensis can produce lipase, which can participate in the decomposition of volatile oil components. The thujone in the wormwood of the present invention is the main toxic component, which is mainly present in the volatile oil. Rhizopus chinensis decomposes or converts thujone by producing lipase to achieve the effect of reducing toxicity; while tanshinone and other components in Salvia miltiorrhiza can cause adverse reactions, Rhizopus chinensis produces glycosidase, oxidoreductase, etc. during the fermentation process, modifies the components in Salvia miltiorrhiza, changes the chemical structure of tanshinone and other components, and reduces the potential risk of adverse reactions while maintaining its efficacy; Bupleurum saponin may cause some toxic reactions at high doses, such as nausea, vomiting, etc., and the hydrolase produced by Rhizopus chinensis may act on Bupleurum saponin to hydrolyze it into small molecule fragments, and its toxicity may be reduced by enzymatic hydrolysis; Rhizopus chinensis can produce glycosidase, and platycoside is a glycoside compound. Glycosidase hydrolyzes the glycosidic bond of platycoside, thereby decomposing it, reducing the adverse reactions such as gastrointestinal irritation that platycoside may cause at high doses; when Rhizopus chinensis ferments Rehmannia glutinosa, it reduces its nourishing nature by decomposing components such as polysaccharides in Rehmannia glutinosa. The nourishing and greasy nature of Rehmannia glutinosa is mainly related to the macromolecular substances such as polysaccharides in its components. The amylase and cellulase produced by Rhizopus chinensis may decompose these components. Similarly, Bacillus subtilis can produce oxidase and hydrolase to modify the chemical structure of thujone, change the double bond or carbonyl structure of thujone through oxidation reaction, or break some of its chemical bonds through hydrolysis reaction, and convert thujone into other relatively low-toxic or non-toxic compounds; similarly, Bacillus subtilis can produce enzymes that catalyze the reduction reaction of tanshinone, so that the chemical structure of tanshinone changes, and the glycosidase produced by Bacillus subtilis can act on bupleurosaponin. During the fermentation of Atractylodes macrocephala, Bacillus subtilis also decomposes the components in Atractylodes macrocephala by secreting a variety of enzymes such as amylase and protease. At the same time, the glycosidase produced by Bacillus subtilis can hydrolyze the glycosidic bonds of Platycodon grandiflorus saponin.

[0026] In terms of improving the efficacy, during the fermentation process, the two fermentations of the present invention allow the microorganisms themselves to produce some metabolites, such as organic acids, amino acids, polypeptides, vitamins, etc. These metabolites synergize with the components in the Chinese herbal medicine to enhance the efficacy. For example, organic acids can increase the solubility of the effective ingredients of the Chinese herbal medicine, making it easier to be absorbed by the human body, and vitamins can assist the drug in exerting its effect in the body and enhance its physiological activity.

[0027] In terms of improving the absorption and utilization efficiency of drugs, fermentation can change the physical and chemical properties of Chinese herbal medicines, such as reducing the particle size of the drug and changing the polarity of the drug. The fermented Chinese herbal extracts may form smaller particles, increase the specific surface area of ​​the drug, speed up the dissolution of the drug in the gastrointestinal tract, and be more easily absorbed by the human body. At the same time, the fermentation process may change the chemical structure of the drug, enhance its affinity with human cells, and thus improve the bioavailability of the drug.

[0028] In a preferred embodiment, in step S5, the mass ratio of the traditional Chinese medicine extract to the enzymatic hydrolyzed fruit and vegetable extract is 1:(0.5-2).

[0029] In a preferred embodiment, in step S5, the method for preparing the fruit and vegetable enzymatic extract is as follows: Lily, spinach, broccoli and celery are ground and mixed with water, cellulase and amylase are added for enzymolysis, and the enzymolysis extracts of fruits and vegetables are obtained by centrifugation and filtration after inactivation.

[0030] In a preferred embodiment, the mass ratio of lily, spinach, broccoli, celery and water is 4:(1-2):(1-2):(1-2):(100-200).

[0031] In a preferred embodiment, based on 100 g of the mass of lily, spinach, broccoli, celery and water, the added amount of the cellulase is 1-4 g, and the added amount of the amylase is 1-4 g.

[0032] In a preferred embodiment, the enzymatic hydrolysis temperature is 30-40°C, and the enzymatic hydrolysis time is 1-2h.

[0033] The invention further adds self-made fruit and vegetable enzymatic extracts into the traditional Chinese medicine extracts, further reducing the toxic components of the traditional Chinese medicines, and can synergistically improve the function of regulating internal organs with the traditional Chinese medicine extracts.

[0034] Specifically, spinach is rich in antioxidant ingredients such as vitamin C, vitamin E, and beta-carotene, which can undergo redox reactions with thujone. Broccoli is rich in sulforaphane, a compound with powerful antioxidant and detoxification functions. Sulforaphane can induce the production of Phase II detoxification enzymes (such as glutathione S-transferase, etc.) in the body; these detoxification enzymes can bind to thujone, making it easier to be excreted from the body; celery contains flavonoids such as apigenin, which have antioxidant and anti-inflammatory properties. Flavonoids can promote the metabolism of thujone by organs such as the liver by regulating the body's metabolic process. The dietary fiber in celery can also promote intestinal peristalsis, accelerate the excretion of thujone and its metabolites, and reduce the residence time of thujone in the body, thereby reducing its harm to the body. Lily itself has a sweet taste and unique flavor that can neutralize the astringency of the above vegetables. Lily has the effects of moistening the lungs and relieving cough, clearing the heart and calming the nerves. Spinach has the effects of nourishing yin and calming the liver, and aiding digestion. Broccoli has the effects of nourishing the kidneys and replenishing essence, strengthening the brain and bones, and celery can clear away heat and dampness, calm the liver and strengthen the stomach. Therefore, the composite Chinese medicine extract of the present invention and the fruit and vegetable enzymatic extract synergistically improve the function of regulating internal organs.

[0035] In a second aspect, an embodiment of the present invention provides an wormwood composition obtained by the method for preparing the wormwood composition for regulating the functions of internal organs as described above.

[0036] In a third aspect, an embodiment of the present invention provides an application of the wormwood composition as described above, wherein the application includes liver regulation, heart regulation, spleen regulation, lung regulation and kidney regulation.

[0037] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The compounded wormwood leaf and various Chinese herbal medicines of the present invention have excellent regulating functions on the internal organs of the human body, and the enzymatic hydrolysis and double fermentation processes completely extract the substances in the Chinese medicine, and the double fermentation further removes the toxic components of the Chinese medicine. In addition, the present invention mixes a variety of fruit and vegetable enzymatic extracts in the extract, and the fruit and vegetable enzymatic extracts can further remove the toxic components in the Chinese medicine, adjust the taste of the obtained preparation, and the fruit and vegetable enzymatic extracts and the Chinese medicine composition synergistically improve the function of regulating the internal organs; 2. The present invention is compounded with a variety of complex enzymes, which can effectively improve the extraction rate of effective ingredients in subsequent fermentation treatment, and can appropriately remove toxic components in some Chinese herbal medicines; 3. The present invention not only removes the toxic components in the Chinese herbal medicine through Rhizopus sinensis and Bacillus subtilis, but also improves the efficacy of the Chinese herbal medicine through the synergy of their metabolites and the Chinese herbal medicine itself, and at the same time improves the absorption and utilization efficiency of the medicine; 4. The present invention further adds homemade fruit and vegetable enzymatic extracts to the traditional Chinese medicine extracts, which further reduces the toxic components of the traditional Chinese medicine and can synergistically improve the function of regulating internal organs with the traditional Chinese medicine extracts. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Unless otherwise specified, the raw materials and reagents used in the present invention are commercially available.

[0040] The comprehensive PDA medium used in the embodiment of the present invention includes 1.2 L of potato boiling liquid, 20 g of glucose, 60 g of straw powder, KH 2 PO 4 2g, MgSO 4 7H 2 O 3g, vitamin B 1 10mg.

[0041] Rhizopus sinensis, numbered SHBCC D20385, was purchased from Wuhan Huaerna Biotechnology Co., Ltd.

[0042] Bacillus subtilis, numbered CGMCC 1.3358, was purchased from Shandong Yihao Biotechnology Co., Ltd.

[0043] Example 1 This embodiment provides a method for preparing a wormwood composition for regulating the functions of internal organs, comprising the following steps: S1, by weight, 65 parts of Artemisia argyi, 23 parts of Salvia miltiorrhiza, 11 parts of Bupleurum chinense, 7 parts of Atractylodes macrocephala, 10 parts of Platycodon grandiflorum, 13 parts of Rehmannia glutinosa, 4 parts of Citrus reticulatae are mixed with 300 parts of water, and a composite enzyme is added, and enzymolysis is performed at 35°C for 14 hours to obtain a solid-liquid mixture, wherein the mass ratio of Artemisia argyi to the composite enzyme is 100:2, the composite enzyme includes cellulase, amylase and pectinase, and the mass ratio of cellulase, amylase and pectinase is 2:0.8:1; S2, adding the solid-liquid mixture to the PDA medium, wherein the mass ratio of the solid-liquid mixture to the components in the PDA medium is 14:100, and inoculating Rhizopus chinensis after mixing, and the viable count of Rhizopus chinensis is 1.9×10 based on the mass of the solid-liquid mixture as 100 g. 11 CFU, one fermentation was carried out, the temperature of one fermentation was 27℃, the time was 36h, and the pH was 7; S3, after inactivation, Bacillus subtilis was inoculated again. The number of viable Bacillus subtilis was 1.5×10 11 CFU, and then a secondary fermentation was carried out at a temperature of 27°C, a time of 28 h, and a pH of 7 to obtain a liquid phase; S4, filtering the liquid phase and concentrating it to obtain a Chinese medicine extract; S5, mixing the Chinese herbal extract with the fruit and vegetable enzymatic extract, wherein the mass ratio of the Chinese herbal extract to the fruit and vegetable enzymatic extract is 1:1, to obtain an Artemisia argyi composition; The preparation method of the fruit and vegetable enzymatic extract is as follows: By weight, 4 parts of lily, 1.5 parts of spinach, 1.5 parts of broccoli and 1.5 parts of celery were ground and mixed with 150 parts of water, cellulase and amylase were added, and the mass of lily, spinach, broccoli, celery and water was 100 g, the amount of cellulase added was 2 g, the amount of amylase added was 2 g, and enzymolysis was carried out at a temperature of 35°C and a time of 1.5 h. After inactivation, the fruit and vegetable enzymatic extracts were obtained by centrifugation and filtration.

[0044] Example 2 This embodiment provides a method for preparing a wormwood composition for regulating the functions of internal organs, comprising the following steps: S1, by weight, 58 parts of Artemisia argyi, 27 parts of Salvia miltiorrhiza, 9 parts of Bupleurum chinense, 6 parts of Atractylodes macrocephala, 8 parts of Platycodon grandiflorum, 9 parts of Rehmannia glutinosa, 4 parts of Citrus reticulatae are mixed with 300 parts of water, and a composite enzyme is added, and enzymolysis is performed at 35°C for 14 hours to obtain a solid-liquid mixture, wherein the mass ratio of Artemisia argyi to the composite enzyme is 100:2, the composite enzyme includes cellulase, amylase and pectinase, and the mass ratio of cellulase, amylase and pectinase is 2:0.8:1; S2, adding the solid-liquid mixture to the PDA medium, wherein the mass ratio of the solid-liquid mixture to the components in the PDA medium is 14:100, and inoculating Rhizopus chinensis after mixing, and the viable count of Rhizopus chinensis is 1.9×10 based on the mass of the solid-liquid mixture as 100 g. 11 CFU, one fermentation was carried out, the temperature of one fermentation was 27℃, the time was 36h, and the pH was 7; S3, after inactivation, Bacillus subtilis was inoculated again. The number of viable Bacillus subtilis was 1.5×10 11 CFU, and then a secondary fermentation was carried out at a temperature of 27°C, a time of 28 h, and a pH of 7 to obtain a liquid phase; S4, filtering the liquid phase and concentrating it to obtain a Chinese medicine extract; S5, mixing the Chinese herbal extract with the fruit and vegetable enzymatic extract, wherein the mass ratio of the Chinese herbal extract to the fruit and vegetable enzymatic extract is 1:1, to obtain an Artemisia argyi composition; The preparation method of the fruit and vegetable enzymatic extract is as follows: By weight, 4 parts of lily, 1.5 parts of spinach, 1.5 parts of broccoli and 1.5 parts of celery were ground and mixed with 150 parts of water, cellulase and amylase were added, and the mass of lily, spinach, broccoli, celery and water was 100 g, the amount of cellulase added was 2 g, the amount of amylase added was 2 g, and enzymolysis was carried out at a temperature of 35°C and a time of 1.5 h. After inactivation, the fruit and vegetable enzymatic extracts were obtained by centrifugation and filtration.

[0045] Comparative Example 1 Compared with Example 1, the difference is that no enzymatic hydrolyzed fruit and vegetable extract is added.

[0046] Comparative Example 2 Compared with Example 1, the difference is that cellulase and amylase are not added in the preparation of the enzymatic hydrolysis extracts of fruits and vegetables.

[0047] Comparative Example 3 Compared with Example 1, the difference is that steps S2 and S3 are changed to: The solid-liquid mixture was added to the PDA medium, wherein the mass ratio of the solid-liquid mixture to the components in the PDA medium was 14:100, and after mixing, Rhizopus chinensis and Bacillus subtilis were inoculated. Based on the mass of the solid-liquid mixture of 100 g, the number of viable bacteria of Rhizopus chinensis was 1.9×10 11 CFU, the number of viable bacteria of Bacillus subtilis is 1.5×10 11 CFU, fermentation, the temperature of the first fermentation was 27℃, the time was 36h, and the pH was 7.

[0048] Comparative Example 4 Compared with Example 1, the difference is that the Rhizopus sinensis in step S2 is replaced by lactic acid bacteria.

[0049] Comparative Example 5 Compared with Example 1, the difference is that the Bacillus subtilis in step S3 is replaced by lactic acid bacteria.

[0050] Clinical efficacy trials: 1. General information: All 210 cases in this group were outpatients, diagnosed as hyperlipidemia by Western medicine and yin deficiency and dampness heat by traditional Chinese medicine, with a course of disease of 1-10 years. They were randomly divided into 7 groups according to the order of entry, namely, the embodiment and the comparative example.

[0051] 2. Diagnostic criteria: refer to "Practical Internal Medicine" and "Guidelines for Clinical Research on New Chinese Medicines for the Treatment of Hyperlipidemia" and formulate in combination with clinical practice. Under normal diet conditions, serum total cholesterol (TC) > 5.68mmol / L, or triglycerides (TG) > 1.71mmol / L, or high-density lipoprotein cholesterol (HDL-C) < 0.83mmol / L tested twice within 4 weeks. With main symptoms such as dizziness, head swelling, headache, or limb numbness. Or accompanied by irritability, red face, dry mouth; or tinnitus, insomnia, forgetfulness; or soreness of waist and knees, fatigue; or palpitations, chest tightness, shortness of breath; or chest and flank distension and pain, stabbing pain in the precordial area; or obesity, abdominal distension, and poor appetite. The pulse may be thin or astringent, and the tongue may be pale or red or have ecchymosis.

[0052] 3. Inclusion and Exclusion Criteria: Inclusion criteria: Anyone who meets the laboratory test indicators and has more than 2 main symptoms and a group of concurrent symptoms can be used as an observation subject; aged between 19-49 years old; informed consent, voluntary participation. Exclusion criteria: pregnant or lactating women; mentally ill patients; combined with severe infection; combined with severe diseases such as gastrointestinal bleeding, cardiovascular and cerebrovascular, liver and hematopoietic system.

[0053] 4. Treatment method: boil and take the Chinese medicine composition of the embodiment and comparative example, three times a day, 15 g each time, for 8 weeks.

[0054] 5. Observation indicators: TC, TG, HDL-C.

[0055] Efficacy evaluation criteria: Markedly effective: blood lipid test results show any one of the following: TC decreases ≥ 20%; TG decreases ≥ 40%; HDL-C increases ≥ 0.26mmol / L (10mg / dl); TC-HDL-C / HDL-C decreases ≥ 20%.

[0056] Effective: Those whose blood lipid test reaches any one of the following items: TC decreases ≥10% but <20%; TG decreases ≥20% but <40%; HDL-C increases ≥0.104mmol (4mg / dl) but <0.26mmol / L (10mg / dl); TC-HDL-C / HDL-C decreases ≥10% but <20%.

[0057] Invalid: Those whose blood lipid test does not meet the above standards.

[0058] The test results are shown in Table 1 and Table 2: Table 1

[0059] Table 2

[0060] From the above clinical trial results, it can be seen that the wormwood composition obtained in Examples 1-2 has excellent internal organs regulating function and has no side effects. The toxic components in the wormwood composition are reduced to be harmless to the human body.

[0061] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a wormwood composition for regulating the functions of internal organs, characterized in that: The following steps are involved: S1, mixing wormwood leaf, salvia miltiorrhiza, bupleurum root, atractylodes macrocephala, platycodon grandiflorum, rehmannia root, tangerine peel and water, and performing enzymolysis to obtain a solid-liquid mixture; S2, mixing the solid-liquid mixture with a culture medium and then inoculating Rhizopus chinensis to perform a fermentation; S3, inoculating Bacillus subtilis after inactivation for secondary fermentation to obtain a liquid phase; S4, filtering the liquid phase and concentrating it to obtain a Chinese medicine extract; S5, mixing the Chinese medicinal extract with the fruit and vegetable enzymatic extract to obtain an Artemisia argyi composition; The fruits and vegetables in the fruit and vegetable enzymatic extract include lily, spinach, broccoli and celery.

2. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 1, characterized in that: In step S1, the mass ratio of wormwood leaf, salvia miltiorrhiza, bupleurum root, atractylodes macrocephala, platycodon grandiflorum, rehmannia root, tangerine peel and water is (50-70): (20-30): (8-13): (5-9): (6-13): (7-20): (2-8): (200-400).

3. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 1, characterized in that: In step S1, the specific steps of enzymolysis are: Add complex enzyme to a mixture of Artemisia argyi, Salvia miltiorrhiza, Bupleurum chinense, Atractylodes macrocephala, Platycodon grandiflorum, Rehmannia glutinosa, Tangerine peel and water, and perform enzymolysis at 30-40°C for 12-18h; The complex enzyme comprises cellulase, amylase and pectinase, and the mass ratio of the cellulase, amylase and pectinase is 2: (0.5-1): (0.5-1); The mass ratio of the wormwood leaf to the complex enzyme is 100:(1-4).

4. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 1, characterized in that: In step S2, the culture medium is PDA culture medium.

5. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 1, characterized in that: In step S2, based on the mass of the solid-liquid mixture being 100 g, the number of viable bacteria of the Rhizopus chinensis is 1×10 10 -2×10 11 CFU; And / or, in step S2, the temperature of the primary fermentation is 25-30°C, the time is 24-72h, and the pH is 6.5-7.5; And / or, in step S3, based on the mass of the solid-liquid mixture being 100 g, the number of viable bacteria of the Bacillus subtilis is 1×10 10 -2×10 11 CFU; And / or, in step S3, the temperature of the secondary fermentation is 25-30°C, the time is 24-72h, and the pH is 6.5-7.

5.

6. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 1, characterized in that: In step S5, the mass ratio of the traditional Chinese medicine extract to the fruit and vegetable enzymatic extract is 1:(0.5-2).

7. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 1, characterized in that: In step S5, the preparation method of the fruit and vegetable enzymatic extract is as follows: Lily, spinach, broccoli and celery are ground and mixed with water, cellulase and amylase are added for enzymolysis, and the enzymolysis extracts of fruits and vegetables are obtained by centrifugation and filtration after inactivation.

8. The method for preparing the wormwood composition for regulating the functions of internal organs according to claim 7, characterized in that: The mass ratio of lily, spinach, broccoli, celery and water is 4: (1-2): (1-2): (1-2): (100-200); And / or, based on 100g of the mass of lily, spinach, broccoli, celery and water, the added amount of the cellulase is 1-4g, and the added amount of the amylase is 1-4g; And / or, the enzymolysis temperature is 30-40° C., and the enzymolysis time is 1-2 h.

9. An wormwood composition obtained by the method for preparing the wormwood composition for regulating the functions of internal organs as described in any one of claims 1 to 8.

10. A use of the wormwood composition as claimed in claim 9, characterized in that: Such applications include liver regulation, heart regulation, spleen regulation, lung regulation, and kidney regulation.

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