Trace element water-soluble fertilizer for improving quality of wheat and preparation method of trace element water-soluble fertilizer

Abstract

The invention relates to a trace element water-soluble fertilizer for improving wheat quality and a preparation method thereof, and belongs to the technical field of water-soluble fertilizer preparation, and the preparation method comprises the following steps: collecting fish feed, pulping, activating composite strains, inoculating a fermentation system containing fish cream, humic acid, potassium dihydrogen phosphate and trace elements, fermenting, and filtering to obtain fermentation liquor. Mixing the fermentation liquor with wood vinegar, adding an anti-flocculation aid prepared from dopamine hydrochloride modified sodium alginate, and stirring at room temperature to obtain the trace element water-soluble fertilizer. The invention provides a trace element water-soluble fertilizer capable of improving the quality of wheat and realizing resource recycling and a preparation method of the trace element water-soluble fertilizer. The fertilizer can comprehensively and stably supply trace elements required by wheat growth, has good anti-flocculation performance, and ensures the uniformity and stability of the fertilizer efficiency. In addition, in the preparation process, renewable resources are fully utilized, the fermentation process is optimized, the fermentation efficiency and the product quality are improved, and the requirements of modern agriculture for efficient and environment-friendly fertilizers are met.

Description

Technical Field

[0001] The present application relates to the technical field of water-soluble fertilizer preparation, and more specifically, to a trace element water-soluble fertilizer for improving wheat quality and a preparation method thereof. Background Art

[0002] In agricultural production, wheat is the main food crop, and the improvement of its quality has always been an important direction of agricultural scientific research. In the traditional wheat planting process, farmers mainly rely on chemical fertilizers to improve soil fertility and promote wheat growth. However, there are many shortcomings in the existing fertilizer technology. On the one hand, traditional fertilizers often use inorganic salts as the main component. Long-term and large-scale use will not only lead to soil structure destruction and fertility decline, but also may cause groundwater pollution and pose a threat to the ecological environment. In addition, the utilization rate of inorganic fertilizers is relatively low, and some nutrients are not effectively absorbed by crops, but are lost with rainwater or fixed in the soil, resulting in resource waste. On the other hand, although some organic fertilizers have appeared on the market in recent years, these fertilizers are often not comprehensive or stable in terms of trace element supply. The demand for trace elements such as zinc, magnesium, and manganese during wheat growth is high, and the content and form of these elements in traditional organic fertilizers are often difficult to meet the optimal needs of wheat growth. At the same time, some organic fertilizers lack effective anti-flocculation technology during the production process, which leads to problems such as caking and stratification of fertilizers during storage and use, affecting the uniformity and stability of fertilizer efficiency.

[0003] Most existing fertilizer preparation technologies fail to fully utilize renewable resources, such as waste fish feed, which not only wastes resources but also increases the risk of environmental pollution. At the same time, traditional fermentation processes have deficiencies in controlling fermentation conditions and optimizing strain selection, resulting in low fermentation efficiency and uneven product quality.

[0004] Based on the above statements, the present application provides a trace element water-soluble fertilizer for improving wheat quality and a preparation method thereof. Summary of the invention

[0005] In order to solve the problems raised in the background technology, the present application provides a water-soluble trace element fertilizer for improving wheat quality and a preparation method thereof, which aims to comprehensively and stably supply the trace elements required for wheat growth, while having good anti-flocculation properties to ensure the uniformity and stability of the fertilizer effect.

[0006] The present application provides a water-soluble trace element fertilizer for improving wheat quality and a preparation method thereof, which adopts the following technical scheme:

[0007] A method for preparing a water-soluble trace element fertilizer for improving wheat quality comprises the following preparation steps:

[0008] S1, collecting fish materials, beating them into pulp, and obtaining fish paste;

[0009] S2, accessing the composite bacteria to an activation medium for activation to obtain a composite fermentation bacteria; then mixing the fish paste obtained in step S1 with a humic acid raw material, potassium dihydrogen phosphate, trace elements, and a fermentation medium to obtain a fermentation system; accessing the composite fermentation bacteria to the fermentation system for fermentation treatment, filtering after fermentation is completed, and collecting the fermentation liquid;

[0010] S3, mixing the fermented liquid in step S2 with wood vinegar, adding an anti-flocculation aid, and stirring at room temperature for 10-30 minutes to obtain a water-soluble trace element fertilizer for improving wheat quality;

[0011] The anti-flocculation aid is obtained by modifying sodium alginate with dopamine hydrochloride.

[0012] Further, in step S1, the fish paste is prepared by the following steps:

[0013] The collected fish feed is rinsed with running water at a temperature below 15°C, then input into a drum meat collector at a temperature of 3-5°C, the pore size of the drum is 3-6mm, then input into a rinsing tank, rinsed at a speed of 30-90rpm for 15-20 minutes, then pre-dehydrated in a rotary screen, and dehydrated in a screw press dehydrator until the water content of the fish feed is 80-85%; the dehydrated fish feed is input into a chopper and processed for 5-10 minutes to obtain fish paste.

[0014] Furthermore, in step S2, the composite fermentation bacteria is prepared by the following steps:

[0015] An activation culture medium is prepared, wherein the culture medium formula is 20 g / L trypsin, 10 g / L yeast extract, 20 g / L glucose, 10 g / L sodium chloride, and the pH value is 6.0-7.0; after the activation culture medium is sterilized, it is inoculated into the composite bacterial strain and cultured on a shaker with an inoculation amount of 6-8%, the culture temperature is set at 28-32°C, the shaking rate is 120-150 rpm, and the fermentation is carried out for 12-16 hours to obtain a composite fermentation bacteria.

[0016] Furthermore, the composite bacteria is obtained by mixing Lactobacillus plantarum, Bacillus subtilis, Bacillus licheniformis and yeast according to the live cell count ratio of (3-5):(5-10):(3-5):(1-3).

[0017] Furthermore, in the fermentation system described in step S2, the usage ratio of fish paste, humic acid raw material, potassium dihydrogen phosphate, trace elements and fermentation medium is (3-5):(5-10):(0.6-1):(0.1-0.5):100.

[0018] Preferably, the humic acid raw material is at least one of furfural residue, earthworm castings, and livestock and poultry manure.

[0019] Furthermore, the trace elements are obtained by mixing amino acid chelated zinc, magnesium salt, manganese salt and copper salt in a mass ratio of 5:(1-3):(1-2):(1-2).

[0020] Furthermore, in step S2, the specific operation of introducing the composite fermentation bacteria into the fermentation system for fermentation treatment is as follows:

[0021] Inoculate the composite fermentation bacteria into the fermentation system at a rate of 10-20%, set the culture temperature to 28-32°C, and after 6-8 hours of fermentation, introduce oxygen into the system at a rate of 0.5-1m 3 / h, and continue fermentation for 20-30 hours.

[0022] In the above reaction process, in the initial fermentation process, an anaerobic environment is provided for the plant lactobacillus in the composite bacteria, and the plant lactobacillus can degrade proteins into macromolecular peptides and amino acids, thereby improving the decomposition effect of fish feed, and the plant lactobacillus can also promote the decomposition of organic matter and produce acidic substances to reduce the pH value of the environment, thereby inhibiting the growth of harmful microorganisms. Subsequently, oxygen is introduced into the system, and the humic acid components produced in the system, and the catechol and pyrogallol functional groups thereon are oxidized into quinones. With the combined action of microbial decomposition and oxidative decomposition, the quinone group is more easily attacked by oxygen, and the aromatic ring is cracked and oxygenated into a carboxyl group, further improving the acidity of the system, the water solubility of humic acid, and the content of oxygen-containing functional groups in the system.

[0023] Furthermore, in step S3, the anti-flocculation aid is specifically prepared by the following steps:

[0024] 0.01-0.02 g of 2-morpholineethanesulfonic acid is added to 50 mL of sterile deionized water, stirred evenly, and the pH value of the system is adjusted to 5.5-7.0 to obtain a 2-morpholineethanesulfonic acid buffer solution; then sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride are dissolved in 20 mL of the 2-morpholineethanesulfonic acid buffer solution, and stirred for 20-30 hours at room temperature under an inert gas atmosphere to obtain an anti-flocculation aid.

[0025] Furthermore, the mass ratio of sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride is (5-8):(0.1-0.3):(0.01-0.2):(0.1-1.0).

[0026] In summary, this application has the following beneficial effects:

[0027] 1. In the technical solution of the present invention, organic and inorganic substances such as fish paste, humic acid raw materials, potassium dihydrogen phosphate, trace elements, etc. are converted into water-soluble fertilizers rich in nutrients and microbial metabolites through a specific microbial fermentation process. These ingredients contribute to the growth and development of wheat, thereby improving the quality of wheat. Using fish feed as a raw material not only reduces the generation of waste, but also realizes the recycling of resources. At the same time, through the microbial fermentation process, fish paste is converted into a high-efficiency fertilizer, increasing its added value. The entire preparation process is environmentally friendly and sustainable, does not produce harmful substances, and is environmentally friendly. At the same time, the raw materials used (such as fish feed, humic acid raw materials, etc.) are renewable resources, which are in line with the concept of sustainable development.

[0028] 2. In the technical solution of the present invention, zinc amino acid chelate is used to compound magnesium salt, manganese salt and other metal salts to form trace elements in water-soluble fertilizers. Zinc is one of the essential trace elements in the growth process of wheat, and has an important influence on the growth and development of wheat. Wheat has a higher demand for zinc than other trace elements. By directly adding zinc amino acid chelate in the fermentation system, the total amount of chelated zinc in the water-soluble fertilizer is increased, and the fertilizer efficiency stability of the water-soluble fertilizer is improved. In addition, the anti-flocculation auxiliary agent prepared by modifying sodium alginate with dopamine hydrochloride, the catechol contained therein can chelate the trace elements in the system through the action of metal ion coordination bonds, increase the content of trace elements that can be accommodated in the water-soluble fertilizer, and prevent the flocculation phenomenon of the fertilizer during preparation and use, further improving the stability and uniformity of the fertilizer.

[0029] 3. In the technical solution of the present invention, fish paste is used as fermentation raw material, and the fermentation process is arranged to promote the soluble humic acid content in liquid water-soluble fertilizer. By accurately controlling fermentation conditions (such as temperature, oxygen ventilation, etc.) and selecting suitable fermentation strains and culture medium, the fermentation process is optimized, and the fermentation efficiency and the quality of the product are improved. Especially in the fermentation process, anaerobic environment is first provided to promote the growth of plant lactobacillus, and then oxygen is introduced to promote the oxidative decomposition of humic acid, further improve the acidity of the system, the water solubility of humic acid and the content of oxygen-containing functional groups in the system, so that the subsequent water-soluble fertilizer obtained has better trace element complexation and better antiflocculation.

[0030] 4. In the technical solution of the present invention, plant lactobacillus, bacillus subtilis, bacillus licheniformis and yeast are mixed to prepare a composite strain. The composite microbial flora is used to ferment fish protein fertilizer, which can integrate the bacterial hydrolysis process and the enzymatic hydrolysis process, with less loss of nutrients, rich beneficial live bacteria, and rich in a variety of beneficial enzymes. The activity of the nutrients in the raw materials can be well retained during the fermentation process, and the utilization rate of the raw materials can be improved. At the same time, based on the broad spectrum and rapid colonization and reproduction of the composite microbial flora, harmful components such as harmful bacteria and worm eggs in the raw materials can be effectively removed, and the safety, effectiveness and usefulness of the fish protein fertilizer can be guaranteed. DETAILED DESCRIPTION

[0031] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The specific implementation methods of this application involve:

[0033] The fish feed used is the waste from tilapia processing;

[0034] The fermentation medium used was LB medium;

[0035] Zinc aspartate: CAS No. 36393-20-1, purity 99.9%;

[0036] Zinc glycinate: CAS No. 14281-83-5, purity 99%;

[0037] Lactobacillus plantarum: CICC 24936;

[0038] Bacillus subtilis: CICC 24713;

[0039] Bacillus licheniformis: CICC 10336;

[0040] Yeast: provided by Shanghai Quanyan Biotechnology Co., Ltd., with a viable count of

[0041] 1.24×10 11 cfu / g.

[0042] Example 1

[0043] A method for preparing a water-soluble trace element fertilizer for improving wheat quality comprises the following preparation steps:

[0044] S1. Collect fish materials, beat them into pulp, and obtain fish paste. The specific operation is as follows:

[0045] The collected fish feed is rinsed with running water at a temperature below 15°C, then input into a drum meat collector at a temperature of 4°C, the aperture of the drum is 5mm, then input into a rinsing tank, rinsed at a speed of 30rpm for 20 minutes, then pre-dehydrated in a rotary screen, and dehydrated in a screw press dehydrator until the water content of the fish feed is 80%; the dehydrated fish feed is input into a chopper and processed for 5 minutes to obtain fish paste.

[0046] S2, inoculating the composite bacteria into an activation medium for activation to obtain composite fermentation bacteria; then mixing the fish paste obtained in step S1 with humic acid raw material, potassium dihydrogen phosphate, trace elements, and fermentation medium to obtain a fermentation system; inoculating the composite fermentation bacteria into the fermentation system at an inoculation rate of 10%, setting the culture temperature to 28°C, and after fermenting for 6 hours, introducing oxygen into the system, and setting the oxygen ventilation volume to 0.5m 3 / h, continue fermenting for 20 hours, filter after fermentation, and collect the fermentation liquid; the usage ratio of fish paste, humic acid raw material, potassium dihydrogen phosphate, trace elements and fermentation medium is 3:5:0.6:0.1:100; the trace elements are obtained by mixing zinc aspartate, magnesium sulfate, manganese sulfate and copper sulfate in a mass ratio of 5:1:1:1; the humic acid raw material is furfural residue;

[0047] Wherein, the composite fermentation bacteria is prepared by the following steps:

[0048] An activation culture medium is prepared, wherein the culture medium formula is 20 g / L trypsin, 10 g / L yeast extract, 20 g / L glucose, 10 g / L sodium chloride, and the pH value is 6.0; after the activation culture medium is sterilized, it is inoculated into a composite bacterial strain and cultured on a shaker, the inoculation amount is 6%, the culture temperature is set to 28°C, the shaking speed is 120 rpm, and the fermentation is carried out for 12 hours to obtain a composite fermentation bacteria; the composite bacterial strain is obtained by mixing Lactobacillus plantarum, Bacillus subtilis, Bacillus licheniformis and yeast according to a live bacterial count ratio of 3:5:3:1.

[0049] S3, the fermented liquid in step S2 is mixed with wood vinegar, the wood vinegar usage amount is 0.5 (v / v)%, and an anti-flocculation aid is added, and the anti-flocculation aid usage amount is 1wt%, and stirred for 10 minutes at room temperature to obtain a trace element water-soluble fertilizer that improves wheat quality;

[0050] Wherein, the anti-flocculation aid is specifically prepared by the following steps:

[0051] 0.01 g of 2-morpholineethanesulfonic acid is added to 50 mL of sterile deionized water, and after stirring evenly, the pH value of the system is adjusted to 5.5 to obtain a 2-morpholineethanesulfonic acid buffer solution; sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride are subsequently dissolved in 20 mL of the 2-morpholineethanesulfonic acid buffer solution, and stirred for 20 hours at room temperature and a nitrogen atmosphere to obtain an anti-flocculation aid; the mass ratio of sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride is 5 g:0.1 g:0.01 g:0.1 g.

[0052] Example 2

[0053] A method for preparing a water-soluble trace element fertilizer for improving wheat quality comprises the following preparation steps:

[0054] S1. Collect fish feed and beat it into pulp to obtain fish paste. The specific operation is as follows:

[0055] The collected fish feed is rinsed with running water at a temperature below 15°C, then input into a drum meat collector at a temperature of 5°C, the aperture of the drum is 5mm, then input into a rinsing tank, rinsed at a speed of 60rpm for 20 minutes, then pre-dehydrated in a rotary screen, and dehydrated in a screw press dehydrator until the water content of the fish feed is 85%; the dehydrated fish feed is input into a chopper and processed for 10 minutes to obtain fish paste.

[0056] S2, inoculating the composite bacteria into an activation medium for activation to obtain composite fermentation bacteria; then mixing the fish paste obtained in step S1 with humic acid raw material, potassium dihydrogen phosphate, trace elements, and fermentation medium to obtain a fermentation system; inoculating the composite fermentation bacteria into the fermentation system at an inoculation rate of 15%, setting the culture temperature to 30°C, and after fermentation for 7 hours, introducing oxygen into the system, setting the oxygen ventilation rate to 0.8m 3 / h, continue fermenting for 25 hours, filter after fermentation, and collect the fermentation liquid; the usage ratio of fish paste, humic acid raw material, potassium dihydrogen phosphate, trace elements and fermentation medium is 4:7:0.8:0.3:100; the trace elements are obtained by mixing zinc aspartate, magnesium sulfate, manganese sulfate and copper sulfate in a mass ratio of 5:2:1:1; the humic acid raw material is furfural residue;

[0057] Wherein, the composite fermentation bacteria is prepared by the following steps:

[0058] An activation culture medium is prepared, wherein the culture medium formula is 20 g / L trypsin, 10 g / L yeast extract, 20 g / L glucose, 10 g / L sodium chloride, and the pH value is 6.5; after the activation culture medium is sterilized, it is inoculated into a composite bacterial strain and cultured on a shaker, the inoculation amount is 7%, the culture temperature is set to 30°C, the shaking speed is 130 rpm, and the fermentation is carried out for 14 hours to obtain a composite fermentation bacteria; the composite bacterial strain is obtained by mixing Lactobacillus plantarum, Bacillus subtilis, Bacillus licheniformis and yeast according to a live bacterial count ratio of 4:8:4:2.

[0059] S3, the fermented liquid in step S2 is mixed with wood vinegar, the wood vinegar usage amount is 0.8 (v / v)%, an anti-flocculation aid is added, and the anti-flocculation aid usage amount is 3wt%, and stirred at room temperature for 20 minutes to obtain a water-soluble trace element fertilizer that improves wheat quality;

[0060] Wherein, the anti-flocculation aid is specifically prepared by the following steps:

[0061] 0.015 g of 2-morpholineethanesulfonic acid was added to 50 mL of sterile deionized water, and after uniform stirring, the pH value of the system was adjusted to 6.1 to obtain a 2-morpholineethanesulfonic acid buffer solution; sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride were then dissolved in 20 mL of the 2-morpholineethanesulfonic acid buffer solution, and stirred for 25 hours at room temperature and a nitrogen atmosphere to obtain an anti-flocculation aid; the mass ratio of sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride was 6.5 g:0.2 g:0.1 g:0.5 g.

[0062] Example 3

[0063] A method for preparing a water-soluble trace element fertilizer for improving wheat quality comprises the following preparation steps:

[0064] S1. Collect fish materials, beat them into pulp, and obtain fish paste. The specific operation is as follows:

[0065] The collected fish feed is rinsed with running water at a temperature below 15°C, then input into a drum meat collector at a temperature of 5°C, the aperture of the drum is 5mm, then input into a rinsing tank, rinsed at a speed of 90rpm for 20 minutes, then pre-dehydrated in a rotary screen, and dehydrated in a screw press dehydrator until the water content of the fish feed is 85%; the dehydrated fish feed is input into a chopper and processed for 10 minutes to obtain fish paste.

[0066] S2, inoculating the composite bacteria into an activation medium for activation to obtain composite fermentation bacteria; then mixing the fish paste obtained in step S1 with humic acid raw material, potassium dihydrogen phosphate, trace elements, and fermentation medium to obtain a fermentation system; inoculating the composite fermentation bacteria into the fermentation system at an inoculation rate of 20%, setting the culture temperature to 32°C, and after fermentation for 8 hours, introducing oxygen into the system, setting the oxygen ventilation volume to 1m 3 / h, continue fermenting for 30 hours, filter after fermentation, and collect the fermentation liquid; the ratio of fish paste, humic acid raw material, potassium dihydrogen phosphate, trace elements and fermentation medium is 5:10:1:0.5:100; the trace elements are obtained by mixing zinc glycine, magnesium sulfate, manganese sulfate and copper sulfate in a mass ratio of 5:3:2:2; the humic acid raw material is earthworm castings;

[0067] Wherein, the composite fermentation bacteria is prepared by the following steps:

[0068] An activation culture medium is prepared, wherein the culture medium formula is 20 g / L trypsin, 10 g / L yeast extract, 20 g / L glucose, 10 g / L sodium chloride, and the pH value is 7.0; after the activation culture medium is sterilized, it is inoculated into a composite bacterial strain and cultured on a shaker with an inoculation amount of 8%, the culture temperature is set at 32°C, the shaking speed is 150 rpm, and the fermentation is carried out for 16 hours to obtain a composite fermentation bacteria; the composite bacterial strain is obtained by mixing Lactobacillus plantarum, Bacillus subtilis, Bacillus licheniformis and yeast in a live bacterial count ratio of 5:10:5:3.

[0069] S3, the fermented liquid in step S2 is mixed with wood vinegar, the wood vinegar usage amount is 1 (v / v)%, an anti-flocculation aid is added, and the anti-flocculation aid usage amount is 5wt%, and stirring is carried out at room temperature for 30 minutes to obtain a water-soluble trace element fertilizer for improving wheat quality;

[0070] Wherein, the anti-flocculation aid is specifically prepared by the following steps:

[0071] 0.02 g of 2-morpholineethanesulfonic acid was added to 50 mL of sterile deionized water, and after being stirred evenly, the pH value of the system was adjusted to 7.0 to obtain a 2-morpholineethanesulfonic acid buffer solution; sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride were subsequently dissolved in 20 mL of the 2-morpholineethanesulfonic acid buffer solution, and stirred for 30 hours at room temperature under an inert gas atmosphere to obtain an anti-flocculation aid; the mass ratio of sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride was 8 g:0.3 g:0.2 g:1.0 g.

[0072] Comparative Example 1

[0073] The difference between this comparative example and Example 1 is that the trace elements in this comparative example are obtained by mixing zinc sulfate, magnesium sulfate, manganese sulfate and copper sulfate in a mass ratio of 5:1:1:1.

[0074] Comparative Example 2

[0075] The difference between this comparative example and Example 1 is that the composite bacteria used in step S2 of this comparative example is obtained by mixing Lactobacillus plantarum, Bacillus subtilis and yeast in a live bacteria ratio of 3:5:1.

[0076] Comparative Example 3

[0077] The difference between this comparative example and Example 1 is that the composite bacteria used in step S2 of this comparative example are obtained by mixing Lactobacillus plantarum, Bacillus subtilis and Bacillus licheniformis in a live bacteria count ratio of 3:5:3.

[0078] Comparative Example 4

[0079] The difference between this comparative example and Example 1 is that the fermentation liquid in step S2 of this comparative example is obtained by the following fermentation steps:

[0080] The composite fermentation bacteria were inoculated into the fermentation system at an inoculation rate of 10%, the culture temperature was set at 28° C., the fermentation was carried out for 26 hours, and after the fermentation was completed, the fermentation liquid was filtered and collected.

[0081] Comparative Example 5

[0082] The difference between this comparative example and Example 1 is that no anti-flocculation aid is used in step S3 of this comparative example.

[0083] Performance Testing

[0084] The trace element water-soluble fertilizers prepared in Examples 1-3 and Comparative Examples 1-5 of the present application were subjected to system stability tests. The water-soluble fertilizer samples prepared in different groups were stored for 72 hours under high temperature and high humidity (40°C, 70RH%), normal temperature and humidity (25°C, 50RH%), and low temperature and low humidity (0°C, 20RH%) conditions, and the changes in the system state were observed to evaluate the stability of different groups. The specific evaluation criteria are as follows:

[0085] Grade A: Completely water-soluble, with no precipitation or suspended matter; no change in pH value.

[0086] Grade B: Mostly soluble in water, with trace amounts of precipitation or suspension; does not affect normal use; pH value changes are less than ±0.5.

[0087] Grade C: There are more precipitates or suspended matter in the system; the pH changes greatly, but is still within the range of 4.5-6.5.

[0088] Grade D: The system is obviously stratified and precipitation is serious; the pH value exceeds the suitable range for crops (4.5-6.5).

[0089] The specific test results are shown in Table 1:

[0090] Table 1

[0091] Group High temperature and high humidity stability Stability at room temperature and humidity Low temperature and low humidity stability Example 1 A A A Example 2 A A A Example 3 A A A Comparative Example 1 A A A Comparative Example 2 C A C Comparative Example 3 B A C Comparative Example 4 A A B Comparative Example 5 C A B

[0092] It can be seen from the results shown in Table 1 above that the comprehensive stability performance of the trace element water-soluble fertilizers prepared in Examples 1-3 of the present application is significantly better than that of the samples prepared in Comparative Examples 1-5, that is, within the technical solution defined in the present application, the stability of the prepared water-soluble fertilizers is excellent.

[0093] Now the fertilizer efficiency of the trace element water-soluble fertilizer prepared in Examples 1-3 and Comparative Examples 1-5 is tested. The water-soluble fertilizers prepared in different groups are sprayed in the wheat test field, and Zhoumai 36, Zhengmai 1860, and Zhengmai 369 are selected as the test wheat varieties. Spray once 3 days after flowering, and topdress once 10 days after flowering, with each spraying amount of 15kg / mu. After harvesting the wheat, the thousand-grain weight of the wheat grains, the protein content of the wheat grains, and the wet gluten content of the wheat grains are counted to evaluate the fertilizer efficiency of different water-soluble fertilizer products. The specific test results are shown in Table 2 below.

[0094] Table 2

[0095]

[0096]

[0097] It can be seen from the results in Table 2 that the water-soluble fertilizer prepared in Examples 1-3 has the best production-promoting effect on the three types of wheat. It can be seen from the results in Comparative Example 1 that increasing the content of chelated zinc in water-soluble fertilizer can improve the production-promoting stability of fertilizer and can better improve the quality of wheat. The results in Comparative Examples 2 and 3 prove the synergistic effect of plant lactobacillus and yeast in decomposing fish feed components, which can better improve the fertilizer efficiency of water-soluble fertilizer; the two types of Bacillus help to increase the content of oxygen-containing functional groups in the system, improve the trace element complexing ability and anti-flocculation effect of fertilizer, and make full use of nutrients in fertilizer. It can be seen from the results in Example 4 that setting the parameters of the fermentation process with composite strains can make the strains have better fertilizer efficiency improvement effects. It can be seen from the results in Comparative Example 5 that the use of anti-flocculation aids can improve the stability and fertilizer efficiency of the water-soluble fertilizer system.

[0098] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0099] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A method for preparing a water-soluble trace element fertilizer for improving wheat quality, characterized in that: The method comprises the following preparation steps: S1, collecting fish materials, beating them into pulp, and obtaining fish paste; S2, accessing the composite bacteria to an activation medium for activation to obtain a composite fermentation bacteria; then mixing the fish paste obtained in step S1 with a humic acid raw material, potassium dihydrogen phosphate, trace elements, and a fermentation medium to obtain a fermentation system; accessing the composite fermentation bacteria to the fermentation system for fermentation treatment, filtering after fermentation is completed, and collecting the fermentation liquid; S3, mixing the fermented liquid in step S2 with wood vinegar, adding an anti-flocculation aid, and stirring at room temperature for 10-30 minutes to obtain a water-soluble trace element fertilizer for improving wheat quality; The anti-flocculation aid is obtained by modifying sodium alginate with dopamine hydrochloride.

2. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 1, characterized in that: In step S1, the fish paste is prepared by the following steps: The collected fish feed is rinsed with running water at a temperature below 15°C, then input into a drum meat collector at a temperature of 3-5°C, the pore size of the drum is 3-6mm, then input into a rinsing tank, rinsed at a speed of 30-90rpm for 15-20 minutes, then pre-dehydrated in a rotary screen, and dehydrated in a screw press dehydrator until the water content of the fish feed is 80-85%; the dehydrated fish feed is input into a chopper and processed for 5-10 minutes to obtain fish paste.

3. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 1, characterized in that: In step S2, the composite fermentation bacteria is prepared by the following steps: S21, prepare activation culture medium, pH value is 6.0-7.0; sterilize the activation culture medium, inoculate the composite bacteria and culture it on a shaker, the inoculation amount is 6-8%, the culture temperature is set to 28-32°C, the shaking speed is 120-150rpm, ferment for 12-16 hours, and obtain composite fermentation bacteria.

4. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 3, characterized in that: The composite bacteria is obtained by mixing Lactobacillus plantarum, Bacillus subtilis, Bacillus licheniformis and yeast according to the live bacteria count ratio of (3-5):(5-10):(3-5):(1-3).

5. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 1, characterized in that: In the fermentation system described in step S2, the dosage ratio of fish paste, humic acid raw material, potassium dihydrogen phosphate, trace elements and fermentation medium is (3-5):(5-10):(0.6-1):(0.1-0.5):

100.

6. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 5, characterized in that: The trace elements are obtained by mixing amino acid chelated zinc, magnesium salt, manganese salt and copper salt in a mass ratio of 5:(1-3):(1-2):(1-2).

7. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 1, characterized in that: In step S2, the specific operation of introducing the composite fermentation bacteria into the fermentation system for fermentation treatment is as follows: Inoculate the composite fermentation bacteria into the fermentation system at a rate of 10-20%, set the culture temperature to 28-32°C, and after fermentation for 6-8 hours, introduce oxygen into the system at a rate of 0.5-1m 3 / h, and continue fermentation for 20-30 hours.

8. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 1, characterized in that: In step S3, the anti-flocculation aid is specifically prepared by the following steps: 2-Morpholineethanesulfonic acid is added to sterile deionized water, stirred evenly, and the pH value of the system is adjusted to 5.5-7.0 to obtain a 2-Morpholineethanesulfonic acid buffer solution; subsequently, sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride are dissolved in the 2-Morpholineethanesulfonic acid buffer solution, and stirred for 20-30 hours at room temperature and inert gas atmosphere to obtain an anti-flocculation aid.

9. The method for preparing a water-soluble trace element fertilizer for improving wheat quality according to claim 8, characterized in that: The mass ratio of sodium alginate, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and dopamine hydrochloride is (5-8):(0.1-0.3):(0.01-0.2):(0.1-1.0).

10. A water-soluble trace element fertilizer prepared by the method for preparing a water-soluble trace element fertilizer for improving wheat quality as claimed in any one of claims 1 to 9.

Images