Biological reaction device and process for producing single-cell protein by using methane-oxidizing bacteria

Abstract

The invention is applicable to the technical field of fermentation engineering, and provides a biological reaction device and process for producing single-cell protein by using methane-oxidizing bacteria, the biological reaction device for producing single-cell protein by using methane-oxidizing bacteria comprises: a tank body for producing single-cell protein by using methane-oxidizing bacteria through methane fermentation, the top of the tank body is provided with a tank cover; the stirring assembly is used for stirring the mixture in the tank body and is arranged in the tank body; the ventilation assembly is mounted on the tank cover and is used for conveying methane and air into the tank body; and the steam sterilizing and disinfecting assembly is used for sterilizing and disinfecting the interior of the tank body by using steam before production. According to the biological reaction device for producing the single-cell protein through the methane-oxidizing bacteria, gas conveying and stirring are combined, equipment is simplified, the steam sterilization function is achieved, the interior of the tank body can be comprehensively sterilized, and use is convenient.

Description

Technical Field

[0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a bioreactor for producing single-cell protein by methane oxidizing bacteria and a process thereof. Background Art

[0002] Single cell protein, also known as microbial protein or bacterial protein, has rich nutritional value, and methane is one of the main sources of greenhouse gases. Methanotrophic bacteria can use methane as the only carbon source and energy source to produce single cell protein.

[0003] Traditionally, in a reaction device where methanotrophic bacteria utilize methane fermentation to produce single-cell protein, not only a gas delivery structure is required, but also a stirring component. The two components work independently, making the equipment structure complicated. In addition, the device lacks a disinfection structure for the tank body, requiring staff to open the tank lid for manual disinfection, which is rather troublesome. Summary of the invention

[0004] In order to solve the technical problems of separate gas delivery and stirring operations, complex equipment and inconvenient tank disinfection in traditional methane-oxidizing bacteria production single-cell protein bioreactors, the present invention provides a methane-oxidizing bacteria production single-cell protein bioreactor and a process thereof.

[0005] The present invention is implemented as follows: a bioreactor for producing single-cell protein by methane oxidizing bacteria, comprising: a tank body for methane oxidizing bacteria to produce single-cell protein by fermenting methane, with a tank cover installed on the top of the tank body; a stirring component for stirring the mixture in the tank body, the stirring component being arranged in the tank body; a ventilation component installed on the tank cover for conveying methane and air into the tank body; and a steam disinfection component for sterilizing the tank body with steam before production.

[0006] Preferably, the tank cover is provided with a feeding port for adding materials into the tank body, and a top cover is provided on the feeding port; the bottom of the tank body is provided with a discharge port, and a switch valve is provided on the discharge port.

[0007] Preferably, the stirring assembly includes: a rotating shaft tube vertically arranged in the tank body; a plurality of stirring rods installed on the rotating shaft tube, and the stirring rods are connected to the rotating shaft tube through a joint; and a stirring drive mechanism arranged on the tank cover for providing driving force for the rotation of the rotating shaft tube.

[0008] Preferably, the stirring drive mechanism includes: a support frame fixed to the top of the tank cover, a sleeve mounted on the top of the rotating shaft tube is fixedly installed on the support frame, and the sleeve is rotatable and sealedly connected to the rotating shaft tube; a stirring motor fixedly installed on the support frame, a first spur gear and a second spur gear are respectively provided on the output shaft of the stirring motor and the rotating shaft tube, and the first spur gear and the second spur gear are meshed with each other.

[0009] Preferably, a gas delivery assembly is arranged above the tank cover, comprising: a tee pipe connected to the top end of the sleeve; a methane input pipe for inputting methane gas and an air input pipe for inputting air, which are respectively connected to the other two ports of the tee pipe, and both the methane input pipe and the air input pipe are provided with a solenoid valve and a flow meter; and the joint piece is T-shaped and is integrally formed by a fixed pipe joint and a rotating pipe joint, one end of the fixed pipe joint is fixedly connected and communicated with one side of the rotating shaft tube, the stirring rod is provided with a mounting limit groove, and the two ends of the rotating pipe joint are respectively rotatably connected to the inner walls on both sides of the mounting limit groove The stirring rod is provided with an inner cavity, and both ends of the rotating pipe joint extend into the inner cavity and are connected with the inner cavity. Both sides of the stirring rod are provided with a plurality of first jet holes connected with the inner cavity, which are used to spray methane and air into the tank body. The stirring rod is provided with a plurality of second jet holes on the side opposite to the rotating shaft tube. The gas sprayed from the second jet holes is expanded from a vertical state to a horizontal state due to the reaction force, so as to stir the material in the tank body when the rotating shaft tube rotates. When the stirring rod is not expanded, that is, in a vertical state, it will not affect the lifting and lowering of the steam disinfection component and will not hinder the steam disinfection operation.

[0010] Preferably, a heating element for heating the material in the tank is installed on the inner wall of the bottom of the tank body, and a temperature sensor for monitoring the temperature inside the tank body is installed at the bottom of the tank body, and a thermostat is provided to control the temperature of the material in the tank body. The connection and control method of the thermostat, the heating element and the temperature sensor belong to the existing mature technology and will not be repeated here.

[0011] Preferably, a mounting plate for installing two adjustment components is provided in the tank body, and the two adjustment components are used to adjust the two steam disinfection components to be aligned close to the inner wall of the tank body for steam disinfection. The steam disinfection components include a mounting tube, a multi-way tube and a plurality of nozzles. The mounting tube is installed on the adjustment component, and the multi-way tube is fixedly connected to one end of the mounting tube, and the plurality of nozzles are installed on the remaining ports of the multi-way tube.

[0012] Preferably, the adjustment assembly includes two first support blocks fixed to the bottom of the mounting plate, a horizontally arranged screw rod is rotatably installed between the two first support blocks, a threaded sleeve on the screw rod is provided with a moving block horizontally slidably connected to the bottom of the mounting plate, and the mounting tube is fixed on the moving block; and the two adjustment assemblies are equipped with an adjustment drive assembly installed on the mounting plate to provide power for the rotation of the two screw rods.

[0013] Preferably, the adjustment drive assembly includes: a first cylindrical cylinder rotatably installed at the center position of the mounting plate, an upper bevel gear and a lower bevel gear being fixedly sleeved on the first cylindrical cylinder; an adjustment motor fixedly installed on the top of the mounting plate and having a second bevel gear fixedly sleeved on the output shaft, the second bevel gear being meshed with the upper bevel gear; two first bevel gears fixedly sleeved on one end of two screws close to each other, the two first bevel gears being meshed with the lower bevel gear.

[0014] Preferably, a mounting seat for installing a rotating mechanism is also installed in the tank body, and the rotating mechanism is used to make the steam disinfection component reciprocate so that the steam can cover the inner wall of the tank for a circle. The rotating mechanism includes: a second cylindrical cylinder rotatably installed at the center position of the mounting seat, and the bottom end of the second cylindrical cylinder is fixedly connected to the top of the mounting plate; a ring gear fixedly mounted on the outer wall of the second cylindrical cylinder; a stepper motor fixedly mounted on the mounting seat and having a main spur gear fixedly mounted on its output shaft, and the main spur gear is meshed with the ring gear.

[0015] Preferably, a steam introduction component is installed on the tank cover for conveying steam to the two steam disinfection components and avoiding obstruction of the reciprocating rotation of the steam disinfection components, the steam introduction component comprises: an upper ring-shaped seat fixedly mounted on the tank cover, a first ring-shaped groove is provided at the bottom of the upper ring-shaped seat; a lower ring-shaped seat rotatably mounted in the first ring-shaped groove of the upper ring-shaped seat through a sealing bearing, a second ring-shaped groove connected to the first ring-shaped groove is provided on the top of the lower ring-shaped seat; two connecting pipes are respectively fixedly mounted on both sides of the lower ring-shaped seat and connected to the second ring-shaped groove, the other ends of the two connecting pipes are fixedly connected with telescopic pipes, and the bottom ends of the telescopic pipes are respectively fixedly connected to one end of the two mounting pipes; a steam input pipe fixedly mounted on the top of the upper ring-shaped seat and connected to the first ring-shaped groove, for connecting to a steam generating device to convey steam through the steam input pipe.

[0016] Preferably, a fixing plate for installing four lifting components is fixedly installed on the top of the tank cover. The lifting component is used to lift the disinfection component so that the disinfection component can be lowered to the bottom of the tank body and the inner wall of the tank body for comprehensive disinfection. The lifting component includes: two second support blocks fixed on the top of the fixing plate; a reel provided between the two second support blocks, a central axis fixedly installed at both ends of the reel, and the two central axes are rotatably connected to the two second support blocks respectively; a pull rope wound on the reel, the bottom end of the pull rope is fixedly connected to the top of the mounting seat; and the four lifting components are equipped with a lifting power mechanism.

[0017] Preferably, the lifting power mechanism includes: a dual-axis motor fixed on the top of the fixed plate, and the two output shafts of the dual-axis motor are fixedly connected to the connecting shaft through a coupling; four sprockets are respectively fixedly mounted on the two connecting shafts and the corresponding two middle shafts, and the four sprockets are connected in pairs by chains, so that the dual-axis motor can synchronously drive the four reels to rotate.

[0018] Preferably, two fixing mechanisms are symmetrically installed on the mounting seat, which are used to fix the mounting seat on the inner wall of the tank body to ensure stability when the mounting plate and the components thereon rotate. The fixing mechanism includes: an L-shaped block fixedly installed on the mounting seat; an electric telescopic rod fixed to one side of the L-shaped block, and a clamping block is fixedly installed on the output rod of the electric telescopic rod, and the two clamping blocks are pressed against the inner walls on both sides of the tank body to keep the mounting seat stable.

[0019] Preferably, a protective cover is fixedly mounted on the tank cover, and the lifting assembly, the lifting power mechanism and the stirring drive mechanism are located inside the protective cover.

[0020] The present invention proposes a process for producing single cell protein using the above-mentioned bioreactor, the process comprising the steps of:

[0021] S. Sterilize and disinfect the inside of the tank:

[0022] First, the steam disinfection component is lowered a certain distance by using the lifting component, that is, the connecting shaft is driven by the double-axis motor, the connecting shaft drives the sprocket to rotate, the sprocket drives the reel to rotate through the chain, and the reel releases the pull rope to lower the mounting seat a certain distance;

[0023] Then, start the two electric telescopic rods to extend the output rod, drive the two pressing blocks to move away from each other until they are pressed against the inner wall of the tank, and fix the mounting seat;

[0024] Start the adjustment drive assembly to rotate the adjustment motor, drive the upper bevel gear and the lower bevel gear on the first cylindrical barrel to rotate, and the lower bevel gear drives the two first bevel gears to rotate, thereby rotating the screw rod, causing the moving block to move, and move the two steam disinfection assemblies away from each other and close to the inner walls on both sides of the tank body, and adjust them to a suitable position so that the subsequent steam can be directly sprayed onto the inner wall of the tank body;

[0025] Then, an external steam generating device can be used to deliver steam through a steam input pipe, and the steam is ejected through a nozzle to sterilize and disinfect the inner wall of the tank;

[0026] At the same time, the stepper motor of the rotating mechanism is started to rotate alternately forward and reverse, so that the main spur gear drives the ring gear to rotate, so that the second cylindrical tube drives the mounting plate to rotate half a circle and then rotate half a circle in the opposite direction, and rotates back and forth in this way, so that the steam disinfection component rotates back and forth to ensure that the steam can cover the inner wall of the tank for one circle;

[0027] Next, follow the above steps and descend a certain distance. The descending distance should be the height of the inner wall of the tank covered by the steam sprayed from the nozzle. Repeat the above steps until the steam disinfection component is lowered to the bottom of the tank to achieve comprehensive disinfection of the inner wall of the tank. Then, perform the reverse operation to restore to the initial state.

[0028] S. Production of single-cell protein using methanotrophic fermentation:

[0029] After disinfection is completed, open the top cover of the feeding port, add methane oxidizing bacteria, nutrients and other materials into the tank through the feeding port, and close the feeding port;

[0030] Then, methane and air are transported into the tank through the ventilation assembly. Methane passes through the methane input pipe, and air passes through the air input pipe. After being mixed at the tee pipe, they enter the sleeve pipe, and then pass through the rotating shaft pipe, the fixed pipe joint on the stirring rod, and the rotating pipe joint, and finally are ejected from the first jet hole and the second jet hole. At the same time, the flow rates of methane and air are controlled and adjusted by the solenoid valve and the flow meter.

[0031] The gas ejected from the second jet hole is expanded from a vertical state to a horizontal state by the reaction force, and the stirring drive mechanism is started. The stirring motor drives the first spur gear to rotate, and the first spur gear meshes with the second spur gear to rotate the shaft tube, thereby driving the stirring rod to stir the mixture in the tank body, so that the methane oxidizing bacteria are fully in contact with methane and air;

[0032] The material in the tank is heated by the heating element at the bottom of the tank, the temperature sensor monitors the temperature, and the temperature controller controls the heating operation of the heating element according to the set temperature to keep the temperature in the tank within a temperature range suitable for the fermentation of single-cell protein by methane oxidizing bacteria;

[0033] During the fermentation process, various parameters in the tank, such as temperature and gas concentration, are continuously monitored, and the gas input of the ventilation component, the stirring speed of the stirring component, and the heating power of the heating element are adjusted according to the actual situation;

[0034] When the fermentation is completed, the switch valve on the discharge port is opened to discharge the material containing the single-cell protein from the discharge port for subsequent single-cell protein separation and purification operations;

[0035] Finally, steam can be delivered through the steam input pipe for cleaning and disinfection in preparation for next use.

[0036] Compared with the related art, the bioreactor for producing single-cell protein by methane oxidizing bacteria and the process thereof provided by the present invention have the following beneficial effects:

[0037] In the conventional device, the gas delivery structure and the stirring assembly work separately, resulting in a complex device structure. The present invention integrates the gas delivery and stirring functions, such as combining the ventilation assembly with the stirring rod. The ventilation assembly delivers methane and air to the stirring rod, and the stirring rod sprays the gas uniformly while stirring. This integration reduces the complex structure of the device, making the structure of the device more compact and simple, and reducing the manufacturing cost and maintenance difficulty of the device.

[0038] Traditional devices lack a tank body disinfection structure and require manual opening of the tank cover for manual disinfection, which is rather troublesome. The steam disinfection component of the present invention can achieve comprehensive and efficient disinfection of the inner wall of the tank through the coordinated action of the adjustment component, the rotating mechanism and the lifting component. There is no need to manually open the tank cover for disinfection, which makes the operation more convenient and reduces the labor intensity of the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 A schematic diagram of the structure of a bioreactor for producing single-cell protein using methane-oxidizing bacteria provided by the present invention;

[0040] Figure 2 A schematic diagram of the front cross-sectional structure of a bioreactor for producing single-cell protein using methane-oxidizing bacteria provided by the present invention;

[0041] Figure 3 for Figure 2 An enlarged structural diagram of part A shown in FIG.

[0042] Figure 4 for Figure 2 An enlarged schematic diagram of the structure of part B shown in FIG.

[0043] Figure 5 for Figure 2 An enlarged schematic diagram of the structure of part C shown in FIG.

[0044] Figure 6 for Figure 2 An enlarged structural diagram of part D shown in FIG.

[0045] Figure 7 for Figure 2 An enlarged structural diagram of part E shown in FIG.

[0046] Figure 8 It is a schematic diagram of the top view of the lifting assembly in the present invention;

[0047] Fig. 9 It is a schematic side view of the steam sterilization component of the present invention;

[0048] Fig.10 It is a three-dimensional assembly diagram of the mounting plate, the mounting seat and the second cylindrical tube in the present invention;

[0049] Fig.11 It is a front view cross-sectional structural schematic diagram of the steam introduction component in the present invention;

[0050] Fig.12 It is a schematic diagram of the three-dimensional structure of the upper ring-shaped seat in the present invention;

[0051] Fig.13 It is a schematic diagram of the three-dimensional structure of the lower annular seat in the present invention;

[0052] Fig.14 It is a three-dimensional assembly diagram of the stirring rod and the vent joint in the present invention;

[0053] Fig.15 It is a side cross-sectional assembly diagram of the stirring rod and the joint member in the present invention;

[0054] Fig.16 This is an assembly diagram of the stirring rod and the rotating shaft tube in the unfolded state in the present invention.

[0055] Figure numerals: 1, tank body; 2, tank cover; 3, shaft tube; 4, stirring rod; 5, support frame; 6, sleeve; 7, stirring motor; 8, first spur gear; 9, second spur gear; 10, three-way pipe; 11, methane input pipe; 12, air input pipe; 13, flow meter; 14, fixed pipe joint; 15, rotating pipe joint; 16, first jet hole; 17, second jet hole; 18, feeding port; 19, discharge port; 20, heating element; 21, mounting plate; 22, mounting pipe; 23, multi-way pipe; 24, nozzle; 25, first supporting block; 26, screw; 27, moving block; 28, first bevel gear; 29, first cylindrical barrel; 30, Adjusting motor; 31. second bevel gear; 32. upper bevel gear; 33. lower bevel gear; 34. mounting seat; 35. second cylindrical barrel; 36. annular gear; 37. stepping motor; 38. main spur gear; 39. upper annular seat; 40. first annular groove; 41. lower annular seat; 42. second annular groove; 43. connecting pipe; 44. telescopic pipe; 45. steam input pipe; 46. fixing plate; 47. second supporting block; 48. middle shaft; 49. reel; 50. pull rope; 51. double-axis motor; 52. connecting shaft; 53. sprocket; 54. chain; 55. L-shaped block; 56. electric telescopic rod; 57. pressing block; 58. protective cover. DETAILED DESCRIPTION

[0056] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of this application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification of this application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification of this application and the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.

[0057] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0058] The embodiment of the present invention provides a bioreactor for producing single-cell protein by methane oxidizing bacteria, such as Figure 1-16 As shown, the bioreactor for producing single-cell protein by methanotrophic bacteria comprises: a tank body 1 for producing single-cell protein by methane fermentation by methanotrophic bacteria, a tank cover 2 being installed on the top of the tank body 1; a stirring assembly for stirring the mixture in the tank body 1, the stirring assembly being arranged in the tank body 1; a ventilation assembly installed on the tank cover 2 for conveying methane and air into the tank body 1; and a steam sterilization assembly for sterilizing the tank body 1 with steam before production.

[0059] In this embodiment, the interior of the tank body 1 is sterilized and disinfected using a steam disinfection component before production. The steam disinfection component can effectively remove the miscellaneous bacteria in the tank body 1, and prevent the miscellaneous bacteria from interfering with the fermentation process of methane oxidizing bacteria to produce single-cell protein, thereby ensuring the purity of the production process and helping to improve the quality of single-cell protein. Then, methane and air are transported into the tank body 1 through the ventilation component on the tank cover 2 to provide the required carbon source and oxygen for the fermentation of methane oxidizing bacteria. At the same time, the stirring component in the tank body 1 stirs the mixture in the tank body 1. During the stirring process, the stirring component can fully mix the methane oxidizing bacteria, nutrients, methane and air, create a uniform growth environment for the methane oxidizing bacteria, and ensure that the methane oxidizing bacteria can fully utilize methane and other nutrients to produce single-cell protein, thereby improving production efficiency.

[0060] In a further preferred embodiment of the present invention, the tank cover 2 is provided with a feeding port 18 for adding materials into the tank body 1, and a top cover is provided on the feeding port 18; a discharge port 19 is provided at the bottom of the tank body 1, and a switch valve is provided on the discharge port 19.

[0061] In this embodiment, during the process of methanotrophic bacteria producing single-cell protein, various required materials, such as nutrients, are conveniently added through the feed port 18 to provide sufficient nutrients for the growth and fermentation of methanotrophic bacteria. A switch valve is provided on the discharge port 19 at the bottom of the tank body 1. When the single-cell protein production is completed, the finished product can be discharged by opening the switch valve of the discharge port 19.

[0062] In a further preferred embodiment of the present invention, the stirring assembly includes: a rotating shaft tube 3 vertically arranged in the tank body 1; a plurality of stirring rods 4 installed on the rotating shaft tube 3, and the stirring rods 4 are connected to the rotating shaft tube 3 through a joint; and a stirring drive mechanism arranged on the tank cover 2 for providing driving force for the rotation of the rotating shaft tube 3.

[0063] In this embodiment, the stirring drive mechanism is arranged on the tank cover 2 to provide driving force for the rotation of the shaft tube 3. The shaft tube 3 rotates vertically in the tank body 1, driving a plurality of stirring rods 4 installed thereon (the stirring rods 4 are connected to the shaft tube 3 through a joint) to stir the mixture in the tank body 1. When the methanotrophic bacteria utilize methane fermentation to produce single-cell protein, the stirring assembly can fully mix the methanotrophic bacteria, methane, air and nutrients in the tank body 1, which is more conducive to the fermentation environment of the methanotrophic bacteria.

[0064] In a further preferred embodiment of the present invention, the stirring drive mechanism includes: a support frame 5 fixed to the top of the tank cover 2, a sleeve 6 sleeved on the top of the rotating shaft tube 3 is fixedly installed on the support frame 5, and the sleeve 6 rotates and is sealed with the rotating shaft tube 3; a stirring motor 7 fixedly installed on the support frame 5, and a first spur gear 8 and a second spur gear 9 are respectively provided on the output shaft of the stirring motor 7 and the rotating shaft tube 3, and the first spur gear 8 and the second spur gear 9 are meshed with each other.

[0065] In this embodiment, the support frame 5 provides support for the stirring motor 7 and other components, and the sleeve 6 fixedly mounted on the support frame 5 is sleeved on the top of the rotating shaft tube 3 and is rotatably sealed. The stirring motor 7 is fixedly mounted on the support frame 5, and the first spur gear 8 of its output shaft is meshed with the second spur gear 9 on the rotating shaft tube 3. During the process of methanotrophic bacteria producing single-cell protein, the stirring motor 7 is started, and the rotating shaft tube 3 is driven to rotate through the meshing of the first spur gear 8 and the second spur gear 9, so that the stirring rod 4 stirs the mixture in the tank body 1.

[0066] In a further preferred embodiment of the present invention, a gas delivery assembly is arranged above the tank cover 2, including: a three-way pipe 10 connected to the top of the sleeve 6; a methane input pipe 11 for inputting methane gas and an air input pipe 12 for inputting air, which are respectively connected to the other two ports of the three-way pipe 10, and the methane input pipe 11 and the air input pipe 12 are both provided with a solenoid valve and a flow meter 13; and the joint piece is T-shaped and is integrally formed by a fixed pipe joint 14 and a rotating pipe joint 15, one end of the fixed pipe joint 14 is fixedly connected and communicated with one side of the rotating shaft tube 3, and the stirring rod 4 is provided with an installation limit groove, and the two ends of the rotating pipe joint 15 are respectively connected to the installation limit groove. The inner walls on both sides of the groove are rotatably connected, the stirring rod 4 is provided with an inner cavity, and both ends of the rotating pipe joint 15 extend into the inner cavity and are connected with the inner cavity. Both sides of the stirring rod 4 are provided with a plurality of first jet holes 16 connected with the inner cavity, which are used to spray methane and air into the tank body 1, and a plurality of second jet holes 17 are provided on the side of the stirring rod 4 opposite to the rotating shaft tube 3. The gas sprayed from the second jet holes 17 is expanded from a vertical state to a horizontal state due to the reaction force, and then the material in the tank body 1 can be stirred when the rotating shaft tube 3 rotates; when the stirring rod 4 is not expanded, that is, in a vertical state, it will not affect the lifting and lowering of the steam disinfection component, and will not cause obstacles to the steam disinfection operation.

[0067] In this embodiment, the three-way pipe 10 of the gas transmission assembly above the tank cover 2 is connected to the top of the sleeve 6, and the methane input pipe 11 and the air input pipe 12 are respectively connected to the other two ends of the three-way pipe 10 to input methane and air, and the solenoid valve and the flow meter 13 control the gas flow. The fixed pipe joint 14 in the joint is connected to the rotating shaft tube 3 and is integrally formed with the rotating pipe joint 15. The rotating pipe joint 15 is connected to the inner cavity of the stirring rod 4, and the first jet holes 16 on both sides of the stirring rod 4 and the second jet holes 17 on one side can spray gas. When the methane oxidizing bacteria produce single-cell protein, methane and air are input as needed and sprayed through the stirring rod 4, which not only provides carbon source and oxygen for the methane oxidizing bacteria, but also can use the reaction force of the sprayed gas to stir the material. When the stirring rod 4 is in a vertical state, it does not affect the steam disinfection assembly. The various components cooperate well during different operations, which helps to improve production efficiency and ensure the smooth progress of the production process.

[0068] In a further preferred embodiment of the present invention, a heating element 20 for heating the material in the tank body 1 is installed on the inner wall of the bottom of the tank body 1, and a temperature sensor for monitoring the temperature in the tank body 1 is installed at the bottom of the tank body 1, and a temperature controller is provided to control the temperature of the material in the tank body 1.

[0069] In this embodiment, the heating element 20 on the inner wall of the bottom of the tank 1 is used to heat the material, the temperature sensor monitors the temperature, and the temperature controller controls the temperature. In the process of methanotrophic bacteria producing single-cell protein, the heating element 20 can provide a suitable temperature, the temperature sensor monitors and accurately controls through the temperature controller, creating ideal temperature conditions for the growth and fermentation of methanotrophic bacteria, which helps to improve the production efficiency and quality of single-cell protein. The connection and control method of the temperature controller, the heating element 20 and the temperature sensor belong to the existing mature technology and will not be repeated here.

[0070] In a further preferred embodiment of the present invention, a mounting plate 21 for mounting two adjustment components is provided in the tank body 1, and the two adjustment components are used to adjust the two steam disinfection components to be aligned close to the inner wall of the tank body 1 for steam disinfection. The steam disinfection component includes a mounting tube 22, a multi-way tube 23 and a plurality of nozzles 24, the mounting tube 22 is mounted on the adjustment component, the multi-way tube 23 is fixedly connected to one end of the mounting tube 22, and the plurality of nozzles 24 are mounted on the remaining ports of the multi-way tube 23.

[0071] In this embodiment, the installation tube 22 in the steam disinfection component is installed on the adjustment component, the multi-way tube 23 is connected to one end of the installation tube 22, and the nozzle 24 is installed at the port of the multi-way tube 23. When the methanotrophic bacteria produce single-cell protein, the adjustment component can make the steam disinfection component close to the inner wall of the tank body 1, and the nozzle 24 directly sprays steam to the inner wall of the tank body 1 for disinfection, ensuring that the inside of the tank body 1 is clean and sanitary, which is conducive to improving the production quality of single-cell protein.

[0072] In a further preferred embodiment of the present invention, the adjustment assembly includes two first support blocks 25 fixed to the bottom of the mounting plate 21, a horizontally arranged screw rod 26 is rotatably installed between the two first support blocks 25, a moving block 27 is threadedly sleeved on the screw rod 26 and horizontally slidably connected to the bottom of the mounting plate 21, and the mounting tube 22 is fixed on the moving block 27; and the two adjustment assemblies are equipped with an adjustment drive assembly installed on the mounting plate 21 to provide power for the rotation of the two screw rods 26.

[0073] In this embodiment, the first support block 25 at the bottom of the mounting plate 21 supports the screw 26. The screw 26 rotates to make the moving block 27 of the threaded sleeve slide horizontally. The mounting tube 22 moves with the moving block 27. The adjusting drive assembly provides power for the rotation of the screw 26. In the process of methanotrophic bacteria producing single-cell protein, the adjusting drive assembly drives the screw 26 to rotate, drives the steam disinfection assembly to move, and can accurately adjust the position of the steam disinfection assembly to ensure that the steam disinfection is comprehensive and effective, which helps to protect the production environment and improve the production quality of single-cell protein.

[0074] In a further preferred embodiment of the present invention, the adjustment drive assembly includes: a first cylindrical tube 29 rotatably mounted at the center position of the mounting plate 21, and an upper bevel gear 32 and a lower bevel gear 33 are fixedly sleeved on the first cylindrical tube 29; an adjustment motor 30 fixedly mounted on the top of the mounting plate 21 and having a second bevel gear 31 fixedly sleeved on the output shaft, and the second bevel gear 31 is meshed with the upper bevel gear 32; two first bevel gears 28 fixedly sleeved on one end of the two screws 26 close to each other, and the two first bevel gears 28 are both meshed with the lower bevel gear 33.

[0075] In this embodiment, the second bevel gear 31 of the output shaft of the adjusting motor 30 on the mounting plate 21 is meshed with the upper bevel gear 32 on the first cylindrical barrel 29, and the lower bevel gear 33 of the first cylindrical barrel 29 is meshed with the first bevel gear 28 on the screw 26. When the methanotrophic bacteria produce single-cell protein, the adjusting motor 30 is started, and the screw 26 is driven to rotate through the meshing between the bevel gears, thereby adjusting the position of the steam disinfection component.

[0076] In another embodiment of the present invention, a mounting base 34 for installing a rotating mechanism is also installed in the tank body 1, and the rotating mechanism is used to make the steam disinfection component reciprocate so that the steam can cover the inner wall of the tank body 1 in a circle. The rotating mechanism includes: a second cylindrical barrel 35 rotatably installed at the center position of the mounting base 34, and the bottom end of the second cylindrical barrel 35 is fixedly connected to the top of the mounting plate 21; a ring gear 36 fixedly mounted on the outer wall of the second cylindrical barrel 35; a stepper motor 37 fixedly mounted on the mounting base 34 and having a main spur gear 38 fixedly mounted on its output shaft, and the main spur gear 38 is meshed with the ring gear 36.

[0077] In this embodiment, the main spur gear 38 on the output shaft of the stepper motor 37 on the mounting seat 34 in the tank body 1 is meshed with the ring gear 36 on the second cylindrical barrel 35, and the second cylindrical barrel 35 is connected to the mounting plate 21. When the methanotrophic bacteria produce single-cell protein, the stepper motor 37 drives the steam disinfection component to reciprocate, and the steam covers the inner wall of the tank body 1 in a circle, which makes the disinfection more comprehensive, ensures the hygiene inside the tank body 1, and helps to improve the production quality of single-cell protein.

[0078] In another embodiment of the present invention, a steam introduction component is installed on the tank cover 2 for conveying steam to the two steam disinfection components and avoiding obstruction of the reciprocating rotation of the steam disinfection components, and the steam introduction component includes: an upper ring-shaped seat 39 fixedly mounted on the tank cover 2, a first annular groove 40 being provided at the bottom of the upper ring-shaped seat 39; a lower annular seat 41 rotatably mounted in the first annular groove 40 of the upper ring-shaped seat 39 through a sealing bearing, a second annular groove 42 communicating with the first annular groove 40 being provided at the top of the lower annular seat 41; two connecting pipes 43 respectively fixedly mounted on both sides of the lower annular seat 41 and communicating with the second annular groove 42, the other ends of the two connecting pipes 43 are fixedly connected with telescopic pipes 44, the bottom ends of the telescopic pipes 44 are respectively fixedly connected to one end of the two mounting pipes 22; a steam input pipe 45 fixedly mounted on the top of the upper ring-shaped seat 39 and communicating with the first annular groove 40, for connecting to a steam generating device to convey steam through the steam input pipe 45.

[0079] In this embodiment, the steam inlet assembly on the tank cover 2, the upper annular seat 39 and the lower annular seat 41 are connected through a sealed bearing, the connecting pipe 43 and the telescopic pipe 44 connect the lower annular seat 41 and the mounting pipe 22, and the steam input pipe 45 connects the upper annular seat 39. When the methanotrophic bacteria produce single-cell protein, the steam of the steam generating device enters through the steam input pipe 45, is transported to the steam sterilization assembly through various components, and does not hinder its reciprocating rotation, ensuring that the steam sterilization is carried out smoothly, helping to maintain the cleanliness of the inside of the tank body 1 and improving the production quality of single-cell protein.

[0080] In another embodiment of the present invention, a fixing plate 46 for mounting four lifting components is fixedly installed on the top of the tank cover 2. The lifting components are used to lift the disinfection components so that the disinfection components can be lowered to the bottom of the tank body 1 and the inner wall of the tank body 1 can be fully disinfected. The lifting components include: two second support blocks 47 fixed on the top of the fixing plate 46; a drum 49 provided between the two second support blocks 47, a central axis 48 is fixedly installed at both ends of the drum 49, and the two central axes 48 are rotatably connected to the two second support blocks 47 respectively; a pull rope 50 wound on the drum 49, and the bottom end of the pull rope 50 is fixedly connected to the top of the mounting seat 34; and the four lifting components are equipped with a lifting power mechanism.

[0081] In this embodiment, the lifting assembly of the fixing plate 46 on the tank cover 2, the reel 49 is installed between the second support block 47 through the central axis 48, the pull rope 50 connects the reel 49 and the mounting seat 34, and the lifting power mechanism is controlled. When the methanotrophic bacteria produce single-cell protein, under the action of the lifting power mechanism, the reel 49 rotates through the pull rope 50 to lift the steam disinfection assembly, which can disinfect the inner wall of the bottom of the tank body 1, ensure comprehensive disinfection, and help improve the production quality of single-cell protein.

[0082] In another embodiment of the present invention, the lifting power mechanism includes: a dual-axis motor 51 fixed on the top of the fixed plate 46, and the two output shafts of the dual-axis motor 51 are fixedly connected with a connecting shaft 52 through a coupling; four sprockets 53 are respectively fixedly mounted on the two connecting shafts 52 and the corresponding two middle shafts 48, and the four sprockets 53 are connected in pairs by chains 54, so that the dual-axis motor 51 can synchronously drive the four reels 49 to rotate.

[0083] In this embodiment, the output shaft of the dual-axis motor 51 on the fixed plate 46 is connected to the connecting shaft 52, the sprocket 53 is sleeved on the connecting shaft 52 and the middle shaft 48, and the chain 54 is connected to the sprocket 53. When the methanotrophic bacteria produce single-cell protein, the dual-axis motor 51 drives the reel 49 to rotate through the sprocket 53 and the chain 54, so that the steam sterilization component is raised and lowered, and the inner wall of the tank body 1 is fully sterilized, the production environment is guaranteed, and it is helpful to improve the production quality of single-cell protein.

[0084] In another embodiment of the present invention, two fixing mechanisms are symmetrically installed on the mounting seat 34, which are used to fix the mounting seat 34 on the inner wall of the tank body 1 to ensure stability when the mounting plate 21 and the components thereon rotate. The fixing mechanism includes: an L-shaped block 55 fixedly installed on the mounting seat 34; an electric telescopic rod 56 fixed to one side of the L-shaped block 55, and a clamping block 57 is fixedly installed on the output rod of the electric telescopic rod 56. The two clamping blocks 57 are pressed against the inner walls on both sides of the tank body 1 to keep the mounting seat 34 stable.

[0085] In this embodiment, in the fixing mechanism on the mounting seat 34, the L-shaped block 55 is connected to the electric telescopic rod 56, and the pressing block 57 is on the output rod of the electric telescopic rod 56. When the methanotrophic bacteria produce single-cell protein, the electric telescopic rod 56 pushes the pressing block 57 to press against the inner wall of the tank body 1, fixes the mounting seat 34, ensures the rotation stability of the mounting plate 21 and its components, and helps to improve production efficiency and quality.

[0086] In another embodiment of the present invention, a protective cover 58 is fixedly installed on the tank cover 2 , and the lifting assembly, the lifting power mechanism and the stirring drive mechanism are located inside the protective cover 58 .

[0087] In this embodiment, the protective cover 58 on the tank cover 2 covers the lifting assembly, the lifting power mechanism and the stirring drive mechanism. When the methanotrophic bacteria produce single-cell protein, the protective cover 58 protects these components from damage or external interference, ensures the smooth progress of the production process, and helps to improve the stability of single-cell protein production.

[0088] The present invention proposes a process for producing single cell protein using the above-mentioned bioreactor, the process comprising the steps of:

[0089] S1. Sterilize and disinfect the interior of the tank 1:

[0090] First, the steam sterilization component is lowered by the lifting component, that is, the connecting shaft 52 is driven by the double-axis motor 51, the connecting shaft 52 drives the sprocket 53 to rotate, the sprocket 53 drives the reel 49 to rotate through the chain 54, and the reel 49 releases the pull rope 50 to lower the mounting seat 34 by a certain distance;

[0091] Then, the two electric telescopic rods 56 are started to extend the output rods, driving the two pressing blocks 57 to move away from each other until they are pressed against the inner wall of the tank body 1, thereby fixing the mounting seat 34;

[0092] Start the adjustment drive assembly to rotate the adjustment motor 30, drive the upper bevel gear 32 and the lower bevel gear 33 on the first cylindrical barrel 29 to rotate, and the lower bevel gear 33 drives the two first bevel gears 28 to rotate, thereby rotating the screw 26, prompting the moving block 27 to move, and move the two steam disinfection components away from each other and close to the inner walls on both sides of the tank body 1, and adjust them to a suitable position so that the subsequent steam can be directly sprayed onto the inner wall of the tank body 1;

[0093] Then, an external steam generating device can be used to deliver steam through the steam input pipe 45, and the steam is sprayed out through the nozzle 24 to sterilize the inner wall of the tank body 1;

[0094] At the same time, the stepper motor 37 of the rotating mechanism is started to rotate alternately forward and reverse, so that the main spur gear 38 drives the ring gear 36 to rotate, so that the second cylindrical tube 35 drives the mounting plate 21 to rotate half a circle and then rotate half a circle in the opposite direction, and rotates back and forth in this way, so that the steam disinfection component rotates back and forth to ensure that the steam can cover the inner wall of the tank body 1 for one circle;

[0095] Next, according to the above steps, descend a certain distance, the descending distance is preferably the height of the inner wall of the tank body 1 covered by the steam sprayed from the nozzle, repeat the above steps until the steam disinfection component is lowered to the bottom of the tank body 1 to achieve comprehensive disinfection of the inner wall of the tank body 1, and then perform the reverse operation to restore to the initial state;

[0096] S2. Production of single-cell protein using methanotrophic fermentation:

[0097] After the disinfection is completed, the top cover of the feed port 18 is opened, and materials such as methane oxidizing bacteria and nutrients are added into the tank body 1 through the feed port 18, and the feed port 18 is closed;

[0098] Then, methane and air are transported into the tank body 1 through the ventilation assembly. Methane passes through the methane input pipe 11, and air passes through the air input pipe 12. After being mixed at the tee pipe 10, they enter the sleeve 6, and then pass through the rotating shaft pipe 3, the fixed pipe joint 14 on the stirring rod 4, and the rotating pipe joint 15, and finally are ejected from the first jet hole 16 and the second jet hole 17. At the same time, the flow rates of methane and air are controlled and adjusted by the electromagnetic valve and the flow meter 13.

[0099] The gas ejected from the second jet hole 17 is expanded from a vertical state to a horizontal state by the reaction force, and the stirring drive mechanism is started. The stirring motor 7 drives the first spur gear 8 to rotate, and the first spur gear 8 meshes with the second spur gear 9, so that the rotating shaft tube 3 rotates, and then drives the stirring rod 4 to stir the mixture in the tank body 1, so that the methane oxidizing bacteria are fully in contact with methane and air;

[0100] The material in the tank body 1 is heated by the heating element 20 at the bottom of the tank body 1, the temperature sensor monitors the temperature, and the temperature controller controls the heating operation of the heating element 20 according to the set temperature to keep the temperature in the tank body 1 within a temperature range suitable for the fermentation of single-cell protein by methane oxidizing bacteria;

[0101] During the fermentation process, various parameters in the tank body 1, such as temperature and gas concentration, are continuously monitored, and the gas input of the ventilation component, the stirring speed of the stirring component, and the heating power of the heating element 20 are adjusted according to the actual situation;

[0102] When the fermentation is completed, the switch valve on the discharge port 19 is opened to discharge the material containing the single cell protein from the discharge port 19 for subsequent single cell protein separation and purification operations;

[0103] Finally, steam can be delivered through the steam input pipe 45 for cleaning and disinfection to prepare for the next use.

[0104] It is worth noting that the circuits, electronic components and modules involved in the present invention are all prior art, and those skilled in the art can fully implement them. Needless to say, the content protected by the present invention does not involve improvements to software and methods.

[0105] This solution is also equipped with a control cabinet. When in use, each electrical device can be started and operated separately through the electric control cabinet. The power connection method of each electrical device is an existing mature technology and a well-known technology for personnel in this field, so no unnecessary details will be given here.

[0106] In summary, compared with the related art, the unique design of the ventilation assembly combined with the stirring rod in the present invention enables methane and air to be efficiently mixed in the material. The ventilation assembly inputs methane and air into the stirring rod, and then ejects them through the first jet hole 16 and the second jet hole 17 on the stirring rod. During the stirring process of the stirring rod, the gas is evenly dispersed, ensuring that there is sufficient methane and air supply around the methane oxidizing bacteria, greatly improving the utilization efficiency of carbon sources and energy by the methane oxidizing bacteria, thereby accelerating the synthesis rate of single-cell protein and improving the overall production efficiency.

[0107] The steam sterilization component can achieve comprehensive and dead-angle sterilization of the inner wall of the tank body 1 through the coordinated work of the adjustment component, the rotating mechanism and the lifting component. Before production, this comprehensive sterilization measure can effectively remove the miscellaneous bacteria in the tank body 1, prevent the miscellaneous bacteria from competing with the methane oxidizing bacteria for nutrients or producing harmful substances during the fermentation process, and ensure the purity and quality stability of the single-cell protein product.

[0108] In the several embodiments provided in this application, it should be understood that the disclosed device can be implemented in other ways.

[0109] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also belong to the scope of protection of the present invention.

Claims

1. A bioreactor for producing single-cell protein from methane-oxidizing bacteria, characterized in that: include: A tank body (1) used for producing single-cell protein by methane fermentation using methanotrophic bacteria, wherein a tank cover (2) is installed on the top of the tank body (1); A stirring component for stirring the mixture in the tank body, the stirring component being arranged in the tank body (1); A ventilation assembly installed on the tank cover (2) for conveying methane and air into the tank body (1); A steam sterilization component is used for sterilizing the inside of a tank body (1) using steam before production.

2. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 1, characterized in that: The tank cover (2) is provided with a feeding port (18) for adding materials into the tank body (1), and a top cover is provided on the feeding port (18); the bottom of the tank body (1) is provided with a discharge port (19), and a switch valve is provided on the discharge port (19).

3. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 1, characterized in that: The stirring assembly comprises: Rotating a rotating shaft tube (3) vertically arranged in the tank body (1); A plurality of stirring rods (4) mounted on the rotating shaft tube (3), wherein the stirring rods (4) are connected to the rotating shaft tube (3) via a joint piece; A stirring drive mechanism is arranged on the tank cover (2) and is used to provide driving force for the rotation of the rotating shaft tube (3).

4. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 3, characterized in that: The stirring drive mechanism comprises: A support frame (5) fixed on the top of the tank cover (2), a sleeve (6) sleeved on the top of the rotating shaft tube (3) being fixedly mounted on the support frame (5), and the sleeve (6) is rotatably and sealingly connected to the rotating shaft tube (3); A stirring motor (7) is fixedly mounted on the support frame (5); a first spur gear (8) and a second spur gear (9) are respectively provided on the output shaft of the stirring motor (7) and the rotating shaft tube (3); the first spur gear (8) and the second spur gear (9) are meshed with each other.

5. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 4, characterized in that: A gas delivery assembly is arranged above the tank cover (2), comprising: A three-way pipe (10) connected to the top of the sleeve (6); A methane input pipe (11) for inputting methane gas and an air input pipe (12) for inputting air are respectively connected to the other two ports of the three-way pipe (10), and both the methane input pipe (11) and the air input pipe (12) are provided with a solenoid valve and a flow meter (13); Furthermore, the joint piece is T-shaped and is formed integrally by a fixed pipe joint (14) and a rotating pipe joint (15); one end of the fixed pipe joint (14) is fixedly connected to and communicated with one side of the rotating shaft tube (3); a mounting limit groove is provided on the stirring rod (4); two ends of the rotating pipe joint (15) are respectively rotatably connected to the inner walls of both sides of the mounting limit groove; the stirring rod (4) is provided with an inner cavity; two ends of the rotating pipe joint (15) extend into the inner cavity and communicate with the inner cavity; a plurality of first jet holes (16) connected to the inner cavity are provided on both sides of the stirring rod (4) for spraying methane and air into the tank body (1); a plurality of second jet holes (17) are provided on the side of the stirring rod (4) opposite to the rotating shaft tube (3).

6. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 1, characterized in that: A heating element (20) for heating the material in the tank (1) is installed on the inner wall of the bottom of the tank (1), and a temperature sensor for monitoring the temperature in the tank (1) is installed at the bottom of the tank (1).

7. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 1, characterized in that: The tank body (1) is provided with a mounting plate (21) for mounting two adjustment components, the two adjustment components are used to adjust the two steam sterilization components to be aligned close to the inner wall of the tank body (1) for steam sterilization, the steam sterilization components include a mounting tube (22), a multi-way tube (23) and a plurality of nozzles (24), the mounting tube (22) is mounted on the adjustment component, the multi-way tube (23) is fixedly connected to one end of the mounting tube (22), and the plurality of nozzles (24) are mounted on the remaining ports of the multi-way tube (23).

8. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 7, characterized in that: The adjustment assembly comprises two first support blocks (25) fixed at the bottom of the mounting plate (21), a horizontally arranged screw rod (26) is rotatably mounted between the two first support blocks (25), a moving block (27) is threadedly sleeved on the screw rod (26) and is horizontally slidably connected to the bottom of the mounting plate (21), and the mounting tube (22) is fixed on the moving block (27); Furthermore, the two adjustment assemblies are equipped with an adjustment drive assembly mounted on the mounting plate (21) to provide power for the rotation of the two screw rods (26).

9. The bioreactor for producing single-cell protein by methanotrophic bacteria according to claim 8, characterized in that: The adjustment drive assembly comprises: A first cylindrical barrel (29) is rotatably mounted at the center of the mounting plate (21), wherein an upper bevel gear (32) and a lower bevel gear (33) are fixedly sleeved on the first cylindrical barrel (29); An adjusting motor (30) is fixedly mounted on the top of the mounting plate (21) and has a second bevel gear (31) on the output shaft fixing sleeve, wherein the second bevel gear (31) is meshed with an upper bevel gear (32); Two first bevel gears (28) are fixedly sleeved on the ends of the two screw rods (26) close to each other, and the two first bevel gears (28) are meshed with the lower bevel gear (33).

10. A process for producing single cell protein using the bioreactor according to claims 1 to 9, characterized in that: The process comprises the steps of: S1. Sterilize and disinfect the interior of the tank (1): First, the steam sterilization component is lowered a certain distance by using the lifting component, that is, the connecting shaft (52) is driven by the double-axis motor (51), the connecting shaft (52) drives the sprocket (53) to rotate, the sprocket (53) drives the reel (49) to rotate via the chain (54), and the reel (49) releases the pull rope (50) to lower the mounting seat (34) a certain distance; Then, the two electric telescopic rods (56) are started to extend the output rod, driving the two pressing blocks (57) to move away from each other until they are pressed against the inner wall of the tank body (1), thereby fixing the mounting seat (34); The adjusting drive assembly is started to rotate the adjusting motor (30), thereby driving the upper bevel gear (32) and the lower bevel gear (33) on the first cylindrical barrel (29) to rotate, and the lower bevel gear (33) drives the two first bevel gears (28) to rotate, thereby causing the screw (26) to rotate, prompting the moving block (27) to move, and the two steam disinfection assemblies are moved away from each other and closer to the inner walls on both sides of the tank body (1), and adjusted to a suitable position so that the subsequent steam can be directly sprayed onto the inner wall of the tank body (1); Then, an external steam generating device can be used to deliver steam through the steam input pipe (45), and the steam is sprayed out through the nozzle (24) to sterilize the inner wall of the tank body (1); At the same time, the stepping motor (37) of the rotating mechanism is started to rotate alternately forward and reverse, so that the main spur gear (38) drives the ring gear (36) to rotate, so that the second cylindrical tube (35) drives the mounting plate (21) to rotate half a circle and then rotate half a circle in the opposite direction, and rotates back and forth in this way, so that the steam disinfection component rotates back and forth, ensuring that the steam can cover the inner wall of the tank once; Then, according to the above steps, the tank is lowered for a certain distance, the lowered distance being preferably the height of the inner wall of the tank body (1) covered by the steam sprayed from the nozzle, and the above steps are repeated until the steam disinfection component is lowered to the bottom of the tank body (1) to achieve full disinfection of the inner wall of the tank body (1), and then the reverse operation is performed to restore the tank body to the initial state; S2. Production of single-cell protein using methanotrophic fermentation: After the disinfection is completed, the top cover of the feeding port (18) is opened, and materials such as methane oxidizing bacteria and nutrients are added into the tank body (1) through the feeding port (18), and the feeding port (18) is closed; Then, methane and air are transported into the tank body (1) through the ventilation assembly. Methane passes through the methane input pipe (11), and air passes through the air input pipe (12). After being mixed at the three-way pipe (10), the methane enters the sleeve (6), passes through the rotating shaft pipe (3), the fixed pipe joint (14) on the stirring rod (4), and the rotating pipe joint (15), and finally is ejected from the first jet hole (16) and the second jet hole (17). At the same time, the flow rates of methane and air are controlled and adjusted by the electromagnetic valve and the flow meter (13); The gas ejected from the second jet hole (17) is expanded from a vertical state to a horizontal state due to the reaction force, and the stirring drive mechanism is started, and the stirring motor (7) drives the first spur gear (8) to rotate, and the first spur gear (8) meshes with the second spur gear (9), so that the rotating shaft tube (3) rotates, and then drives the stirring rod (4) to stir the mixture in the tank body, so that the methane oxidizing bacteria are fully in contact with methane and air; The material in the tank body (1) is heated by a heating element (20) at the bottom of the tank body, the temperature is monitored by a temperature sensor, and the temperature controller controls the heating operation of the heating element (20) according to the set temperature, so as to keep the temperature in the tank body within a temperature range suitable for the production of single-cell protein by methane oxidizing bacteria fermentation; During the fermentation process, various parameters in the tank, such as temperature and gas concentration, are continuously monitored, and the gas input of the ventilation component, the stirring speed of the stirring component, and the heating power of the heating element (20) are adjusted according to actual conditions; When the fermentation is completed, the switch valve on the discharge port (19) is opened to discharge the material containing the single cell protein from the discharge port (19) for subsequent single cell protein separation and purification operations; Finally, steam can be delivered through the steam input pipe (45) for cleaning and disinfection, so as to prepare for the next use.

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