Odor-removing denitrifying bacteria and application thereof in composite intelligent water purification

Abstract

The invention belongs to the technical field of advanced treatment and recycling of aquaculture tail water, and particularly relates to an odor-removing denitrifying bacterium and application thereof in composite intelligent water purification. The peculiar smell removing and denitrifying bacteria are glutamicum sp. YTLJ-N-S7, the preparation method of the peculiar smell removing and denitrifying bacteria comprises the following steps: screening to obtain the glutamicum sp. YTLJ-N-S7, pre-culturing and propagating the glutamicum sp. YTLJ-N-S7, carrying out vacuum freeze drying to obtain corresponding single-bacterium powder, mixing and assembling the single-bacterium powder and enterococcus sp. YTLJ-N-SXH1 / nitrifying bacteria, and mixing with an auxiliary agent to obtain the peculiar smell removing and denitrifying bacteria. A peculiar smell and nitrogen removal microbial agent is added into the aquaculture system, part of tail water to be treated flows through an external peculiar smell and nitrogen removal equipment system to be treated, and the treated aquaculture tail water is regenerated and reused.

Description

Technical Field

[0001] The present invention belongs to the technical field of deep treatment and resource utilization of aquaculture tail water, and specifically relates to a deodorizing and denitrifying bacterium and its application in composite intelligent water purification. Background Art

[0002] Problems such as water pollution and frequent aquatic diseases caused by large-scale high-density aquaculture have seriously troubled the development of the aquaculture industry. At present, there are few water treatment and recycling technologies in high-density aquaculture systems, the treatment costs are high, the nitrogen removal effect is average, and it is easy to cause the accumulation of odorous substances, which greatly limits the promotion and application of recirculating water aquaculture technology.

[0003] In recent years, in-situ water purification technology based on biofloc farming has been used in the aquaculture industry to a certain extent. This technology can not only achieve rapid conversion of ammonia nitrogen in the breeding pond and purify the water quality, but also can be used as a high-protein bait for aquatic animals, thereby reducing feed costs. By searching the existing technologies, it was found that Deng Yingneng et al. mentioned in the article "Formation conditions and effects of biofloc in closed culture experiment of Penaeus vannamei" that by introducing biofloc technology into the closed culture system of Penaeus vannamei, the concentrations of ammonia nitrogen and nitrite nitrogen in the water body were maintained at a low level, and the survival rate of shrimp was above 80% (Advances in Fisheries Science, 2012, 33(2): 69-75); Li Bin et al. mentioned in the article "Effects of biofloc on water quality regulation and growth of juvenile sea cucumbers (Apostichopus japonicus)" that the biofloc formed by adding carbon source and Bacillus can not only purify water quality, but also promote the growth of juvenile sea cucumbers (Advances in Fisheries Science, 2014, 46(1): 197-205). However, the above technologies all promote the assimilation of ammonia nitrogen into organic nitrogen by adding a large amount of organic carbon sources as exogenous sources. The biofloc production in the aquaculture system is high and the oxygen consumption is high. At the same time, the long-term accumulation of feces and residual bait in the aquaculture system is more likely to cause the accumulation of odor substances, thus affecting the quality of aquatic products. These shortcomings limit the promotion and application of high-density aquaculture technology. Summary of the invention

[0004] The purpose of the present invention is to provide a deodorizing and denitrifying bacteria and its application in composite intelligent water purification in view of the deficiencies of the prior art.

[0005] To achieve the above object, the technical solution adopted by the present invention is: The deodorizing and denitrifying bacteria is Glutamicibacter sp. YTLJ-N-S7, which was deposited in the Guangdong Provincial Microbiological Culture Collection Center on May 26, 2021, with the collection number GDMCC No: 61694, the classification name is Glutamicibacter sp., and the collection address is Guangzhou, China.

[0006] The application of the Glutamicibacter sp. YTLJ-N-S7 in water purification, deodorization and denitrification.

[0007] A water purification, odor removal and denitrification bacterial agent, the bacterial agent contains the Glutamicibacter sp. YTLJ-N-S7.

[0008] The bacterial agent is one or more of Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 or nitrifying bacteria. The Enterococcus sp. YTLJ-N-SXH1 was deposited in the Guangdong Provincial Microbiological Culture Collection Center on November 26, 2021, with the collection number GDMCC No: 62080, and the classification name is Enterococcus sp., and the collection address is Guangzhou, China.

[0009] The Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1, and nitrifying bacteria are propagated separately, dried to obtain dry powders of the strains, and then the propagated Glutamicibacter sp. YTLJ-N-S7 dry powder and Enterococcus sp. YTLJ-N-SXH1 dry powder are mixed at a mass ratio of 1 to 10:1 to obtain bacterial powder A; Glutamicibacter sp. YTLJ-N-S7 dry powder and nitrifying bacteria dry powder are mixed in a mass ratio of 1 to 10:1 to obtain bacterial powder B; The above-mentioned bacterial powders were mixed with the auxiliary agents at a mass ratio of 1:1 to 100 to obtain the bacterial agents, and the total effective viable count of each bacterial agent was ≥1.0×10 9 Pieces / mL; Alternatively, the bacterial powders are mixed with the auxiliary agents respectively, and the obtained mixtures are mixed again to obtain the bacterial agent.

[0010] The auxiliary agent is a mixture of sterilized livestock manure bait and wheat bran, soybean meal and bagasse in a mass ratio of 1:1:1:1; after mixing, 0.1% to 1.0% by mass of expanded Glutamicibacter sp. YTLJ-N-S7 dry powder (a mixture of sterilized livestock manure bait and wheat bran, soybean meal and bagasse) is added, and then 0.05% to 0.5% by mass of mixture A is added, and after mixing, the water content of the reaction system is controlled to be 40% to 80%, and then fermentation is carried out, the fermented product is collected and 0.5-1.0% by mass of polyglutamic acid is added to mix to prepare the auxiliary agent; wherein the mixture A is a mixture of NH4NO3 and glutamic acid in a mass ratio of 1:1 to 5.

[0011] An application of the bacterial agent, the application of the bacterial agent in water purification, odor removal and nitrogen removal.

[0012] The bacterial agent is used in composite intelligent treatment of water inside and outside aquaculture ponds, deodorization, and denitrification.

[0013] The composite intelligent treatment of water inside and outside the breeding pond refers to using the bacterial agent obtained by mixing bacterial powder A with an auxiliary agent to treat the in-situ internal circulating water of the breeding pond; and using the bacterial agent obtained by mixing bacterial powder B with an auxiliary agent to treat the external circulating tail water outside the breeding pond.

[0014] The inoculation amount of the bacterial agent in the treatment, deodorization and denitrification of the in-situ circulating water in the aquaculture pond is 1.0 to 10.0 g / m 2 ; The inoculation amount of the bacterial agent in the treatment, deodorization and denitrification of the external circulating tail water of the aquaculture pond is 0.1-1.0 g / m 2 .

[0015] During the treatment, deodorization and denitrification of the in-situ circulating water in the aquaculture pond, a temperature, dissolved oxygen and pH intelligent control system is installed in the aquaculture pond. The dissolved oxygen of the control system is not less than 5 mg / L, and the pH value is not less than 6.5 and not higher than 8.5, ensuring that the internal circulation system is within the suitable environment for the growth of aquaculture organisms and water purification by bacterial agents.

[0016] During the treatment, odor removal and denitrification of the externally connected tail water of the aquaculture pond, a dissolved oxygen and pH intelligent control system is installed outside the aquaculture pond. Aeration is stopped when the dissolved oxygen in the tail water is higher than 5 mg / L, and the pH value is not lower than 6.0 and not higher than 9.0. Ensure that the external circulation system is within the suitable environment for bacterial agent water purification.

[0017] The externally connected external circulation tail water odor removal, denitrification and reuse system (external circulation system) is also equipped with an electric flocculation or microfiltration device at the front end, and an ozone or ultraviolet disinfection system at the end.

[0018] The advantages of the present invention are: The probiotics that can remove nitrogen, remove odor and inhibit pathogens are combined to realize the integration of denitrification, disinfection and deodorization. Specifically: 1) Compared with common organisms, the Glutamicibacter sp. YTLJ-N-S7 used in the present invention can degrade odorous substances while biologically removing nitrogen, and has the dual functions of removing odor and removing nitrogen.

[0019] 2) The Glutamicibacter sp. YTLJ-N-S7 of the present invention is mixed with common nitrifying bacteria to achieve complementary advantages in nitrogen metabolism pathways, which is beneficial to improving the denitrification efficiency and shock resistance of the system. The special denitrification pathway of the Glutamicibacter sp. YTLJ-N-S7 of the present invention is completely different from the pathway from ammonia nitrogen to nitrate of common nitrifying bacteria. It has the special function of heterotrophic short-term nitrification and denitrification-aerobic denitrification. It can convert ammonia nitrogen in wastewater into hydroxylamine through heterotrophic short-term nitrification and denitrification, and then quickly generate nitrogen gas for removal, reducing the production of highly toxic nitrite. At the same time, it can completely remove nitrite and nitrate from the aquaculture water body in the form of nitrogen gas under aerobic conditions through aerobic denitrification, further avoiding the accumulation of nitrite and nitrate in the system.

[0020] 3) The present invention adopts a special new strain that can produce glutamic acid, which has the effect of increasing the freshness and improving the palatability of bait, thereby increasing the feeding rate of aquatic animals, saving feed, and realizing the recycling of bait. It can solve the problem of feces and residual bait retention in aquaculture water in an eco-friendly manner and reduce environmental pollution.

[0021] 4) The Glutamicibacter sp. YTLJ-N-S7 of the present invention can form a mutually beneficial symbiotic relationship with Enterococcus sp. YTLJ-N-SXH1 in the aquaculture system and the aquaculture organisms, inhibit pathogenic bacteria and promote the proliferation of Glutamicibacter sp. YTLJ-N-S7 in the intestines and feces, achieve the effect of in-situ degradation and deodorization of odorous substances, ensure the simple operability of in-situ treatment and purification of aquaculture water, and at the same time enhance the immunity of aquaculture organisms.

[0022] 5) The Glutamicibacter sp. YTLJ-N-S7 of the present invention is intelligently assembled with other functional strains to create a specific water purification bacterial agent suitable for the internal and external double circulation system. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a cluster analysis diagram of Glutamicibacter sp. YTLJ-N-S7 and other strains of the same genus provided in an embodiment of the present invention.

[0024] Figure 2 This is a structural diagram of a breeding pond provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0025] The present invention is further illustrated by the following examples, but the present invention is not limited to the following examples.

[0026] The nitrifying bacteria mentioned in the following examples are commercially available strains. Example 1

[0027] Isolation of Glutamicibacter sp. YTLJ-N-S7: The Glutamicibacter sp. YTLJ-N-S7 was isolated from the coastal environment, and the specific isolation process was as follows: 10 g of water sample was placed in 50 mL of sterile water, and the mixture was oscillated at a constant temperature of 25° C. for 48 hours. The bacterial solution was centrifuged at 8000 rpm for 10 minutes, and then the upper clear liquid was removed. The lower bacterial solution was transferred to a sterilized enrichment medium and cultured at 30° C. for 7 days, and then diluted and cultured in LB medium. Single colonies were selected and streaked on agar solid medium to obtain the purified strain Glutamicibacter sp. YTLJ-N-S7.

[0028] The enrichment medium used was a mass ratio of 0.08% NH4NO3, 0.05% NH4NO2, 0.2% sodium acetate, 0.1% glutamic acid, 3% K2HPO4, 1% MgSO4, 2% NaCl, 0.03% FeSO4, 0.02% MnSO4, geosmin (100 μg / L) and 2-methylisoborneol (100 μg / L).

[0029] The Glutamicibacter sp. YTLJ-N-S7 was deposited in the Guangdong Provincial Microbiological Culture Collection Center on May 26, 2021, with the collection number GDMCC No: 61694, the classification name is Glutamicibacter sp., and the collection address is Guangzhou, China.

[0030] By comparing the 16S rDNA gene sequence, it was confirmed that Glutamicibacter sp. YTLJ-N-S7 was a newly discovered species.

[0031] The 16S rDNA gene sequence of the Glutamicibacter sp. YTLJ-N-S7 is: Example 2

[0032] Application of the above-obtained Glutamicibacter sp. YTLJ-N-S7 in water purification systems: Glutamicibacter sp. YTLJ-N-S7 pure bacteria were cultured in enriched medium at 30°C until OD 600 ≥1.0, and then the bacterial liquid was inoculated into the simulated wastewater at an inoculation rate of 10wt%, wherein the wastewater was the tail water of a sewage treatment plant with added ammonia nitrogen, nitrite, nitrate, geosmin and glucose, wherein the concentrations of ammonia nitrogen, nitrite and nitrate nitrogen were 1, 0.5 and 50mg / L respectively, the concentrations of odor substances geosmin and 2-methylisoborneol were both 100ng / L, and the concentration of glucose was 200mg / L.

[0033] After inoculation of the bacterial liquid and aerobic treatment at 25°C for 48 hours, the concentrations of ammonia nitrogen, nitrite and nitrate nitrogen dropped to 0.02, 0.01 and 1 mg / L respectively, geosmin and 2-methylisoborneol were both lower than 10 ng / L, and glucose was lower than 1 mg / L; at the same time, 1 mg / L of glutamate and 0.02 mg / L of hydroxylamine were produced.

[0034] Compared with common nitrifying bacteria, Glutamicibacter sp. YTLJ-N-S7 exhibits heterotrophic short-term nitrification and denitrification-aerobic denitrification functions. The loss of organic carbon source (glucose) and the production of hydroxylamine confirm the occurrence of heterotrophic short-term nitrification and denitrification by Glutamicibacter sp. YTLJ-N-S7. The removal of nitrate under aerobic conditions confirms the occurrence of aerobic denitrification by Glutamicibacter sp. YTLJ-N-S7. At the same time, Glutamicibacter sp. YTLJ-N-S7 overcomes the shortcomings of common denitrifying bacteria that cannot remove ammonia nitrogen and achieves the removal of ammonia nitrogen. Example 3

[0035] Deep treatment and recycling of aquaculture water in an eel farming process in a coastal area: 1) Preparation method of deodorizing and denitrifying bacterial agent a) Strain propagation: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 and nitrifying bacteria were inoculated into the propagation medium at an inoculation amount of 5wt%, and cultured at 30°C for 24 hours. After the culture time, the bacteria were collected and set aside. The culture medium for strain propagation is glutamic acid (0.02%), glucose (0.02%), and the balance is water in terms of mass percentage.

[0036] Nitrifying bacteria were purchased from Huaxu Co., Ltd. Enterococcus sp. YTLJ-N-SXH1 was deposited in Guangdong Microbiological Culture Collection Center on November 26, 2021, with the deposit number GDMCC No: 62080, the classification name is Enterococcus sp., and the deposit address is Guangzhou, China.

[0037] b) Preparation of mixed bacterial powder: The bacterial solutions of Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 and nitrifying bacteria obtained by the above-mentioned expansion were freeze-dried (pre-cooled at -20°C for 2h, freeze-dried at -50°C for 6h) to obtain dry powder of single strain.

[0038] Then, the dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of Enterococcus sp. YTLJ-N-SXH1 were mixed in a ratio of 1:1 to prepare an internal circulation type odor removal and denitrification mixed bacterial powder; the dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of nitrifying bacteria were mixed in a ratio of 1:1 to prepare an external circulation type odor removal and denitrification mixed bacterial powder.

[0039] c) Preparation of auxiliary agent: Take the residual bait of farmed feces (obtained from farmed eels in this embodiment) and sterilize it with potassium bisulfate in a conventional manner, then mix it with wheat bran, soybean meal and bagasse. The mass mixing ratio of the disinfected residual bait of feces, wheat bran, soybean meal and bagasse is 1:1:1:1. After mixing, add 0.1% (mass) of the dry powder of Glutamicibacter sp. YTLJ-N-S7 expanded according to the above steps and 0.05% (mass) of the mixture to the system, control the water content of the reaction system to 40% after mixing, and then ferment (fermentation conditions are 35°C for 5 days). After the fermentation is completed, collect the fermented product, dry it, add 0.5% of the mass of polyglutamic acid of the fermented product and mix to prepare an auxiliary agent.

[0040] Wherein, NH4NO3 and glutamic acid are mixed in the mixture in a mass ratio of 1:1.

[0041] d) Preparation of bacterial agent: The internal circulation type mixed bacterial powder or the external circulation type mixed bacterial powder obtained in step b is mixed with the auxiliary agent obtained in step c in a ratio of 1:1 (mass ratio) to prepare an internal circulation type deodorizing and denitrifying bacterial agent and an external circulation type deodorizing and denitrifying bacterial agent, respectively.

[0042] 2) Start-up and operation of composite water purification process system a) The place to be treated is an eel breeding pond. After 180 days of breeding eels in the breeding pond, the water body is used as the water body of the breeding pond in-situ odor removal and denitrification system (internal circulation system); after the internal circulation treatment in the breeding pond, the water body flows out from the outlet of the breeding pond and enters the external tail water treatment pool, which is used as the external tail water odor removal, reuse and denitrification system (external circulation system).

[0043] Among them, the water quality indicators in the internal circulation system are as follows: COD concentration 65.4 mg / L, nitrate concentration 12.4 mg / L, nitrite concentration 0.7 mg / L, ammonia nitrogen concentration 1.4 mg / L, and 2-methylisoborneol concentration 300 ng / L.

[0044] b) Add internal circulation deodorizing and denitrifying bacteria to the in-situ deodorizing and denitrifying system (internal circulation system) of the aquaculture pond. The inoculation amount of the bacteria is 1.0 g / m 2 .

[0045] c) The in-situ odor removal and denitrification system (internal circulation system) of the aquaculture pond is equipped with an intelligent feeding control system, temperature, dissolved oxygen and pH intelligent control system. The dissolved oxygen of the control system is not less than 5 mg / L; the pH value is not less than 6.5 and not higher than 8.5, ensuring that the internal circulation system is in the appropriate environment for the growth of aquaculture organisms and water purification by bacterial agents (see Figure 2 ).

[0046] After the above in-situ treatment, the water quality of the breeding pond was tested, and the COD concentration was 22 mg / L, the nitrate concentration was 8.8 mg / L, the nitrite concentration was 0.5 mg / L, the ammonia nitrogen concentration was 1.0 mg / L, and the 2-methylisoborneol concentration was 200 ng / L.

[0047] d) The external tail water deodorization and denitrification recycling system (external circulation system) is equipped with fillers (see Figure 2 ), and inoculate the external circulation deodorizing and denitrifying bacteria on the filler, the inoculation amount of the bacteria is 0.1g / m 2 . An electrocoagulation-microfiltration device is installed at the front end of the external tail water deodorization and denitrification reuse system (external circulation system), and an ozone or ultraviolet disinfection system is installed at the end; at the same time, a dissolved oxygen and pH intelligent control system are installed. When the dissolved oxygen in the tail water is higher than 5mg / L, aeration is stopped, and the pH value is not lower than 6.0 and not higher than 9.0. Ensure that the external circulation system is in the suitable environment range for bacterial agent water purification.

[0048] After external circulation treatment, the effluent COD concentration is 6.4mg / L, nitrate concentration is 2.1mg / L, nitrite concentration is 0.12mg / L, ammonia nitrogen concentration is 0.13mg / L, and 2-methylisoborneol concentration is 10ng / L. The removal rates of the composite water purification process system are COD≥90%, ammonia nitrogen≥90%, nitrite≥80%, nitrate≥80%, and 2-methylisoborneol≥96%. The treated tail water is returned to the aquaculture pond for recycling.

[0049] e) The aquaculture tail water is treated in situ by the aquaculture pond in-situ odor removal and denitrification system (internal circulation system). After the in-situ treatment, part of the tail water is diverted to the external tail water odor removal and denitrification reuse system (external circulation system) for further purification and treatment, and finally flows back to the aquaculture pond. Example 4

[0050] Application of odor removal and denitrification composite water purification process in shrimp farming in the Yellow River Delta: 1) Preparation method of deodorizing and denitrifying bacterial agent a) Strain propagation: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1, and nitrifying bacteria were inoculated into the propagation medium at an inoculation amount of 10wt%, and then cultured at 20°C for 72 hours. After the culture time, the bacteria were collected. The culture medium for strain propagation is glutamic acid (0.2%), glucose (0.2%), and sucrose (1%) by mass percentage, and the balance is water.

[0051] b) Preparation of mixed bacterial powder: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 and nitrifying bacteria obtained by propagation were freeze-dried separately to obtain single strain dry powder of each strain.

[0052] Then, the dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of Enterococcus sp. YTLJ-N-SXH1 were assembled and mixed in a ratio of 2:1 to prepare an internal circulation type odor removal and denitrification mixed bacterial powder; the dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of nitrifying bacteria were assembled and mixed in a ratio of 3:1 to prepare an external circulation type odor removal and denitrification mixed bacterial powder.

[0053] c) Preparation of auxiliary agent: Take the residual bait of aquaculture feces (obtained from aquacultured shrimp in this embodiment) and sterilize it with potassium bisulfate in a conventional manner, then mix it with wheat bran, soybean meal and bagasse. The mass mixing ratio of the residual bait of feces, wheat bran, soybean meal and bagasse is 1:1:1:1. After mixing, add 0.4% of the mass of expanded Glutamicibacter sp. YTLJ-N-S7 dry powder to the mixture, and then add 0.1% of the mass of the mixture. After mixing, control the water content of the reaction system to 50%, and then ferment. After the fermentation is completed, collect the fermented product, dry it, add 0.6% of the mass of polyglutamic acid to the fermented product, and mix to prepare an auxiliary agent.

[0054] Wherein, NH4NO3 and glutamic acid are mixed in the mixture at a mass ratio of 1:3; d) Preparation of bacterial agent: The internal circulation deodorizing and denitrifying mixed bacterial powder or the external circulation deodorizing and denitrifying mixed bacterial powder obtained in step b is mixed with the auxiliary agent obtained in step c at a ratio of 1:10 (mass ratio) to prepare an internal circulation deodorizing and denitrifying bacterial agent and an external circulation deodorizing and denitrifying bacterial agent, respectively.

[0055] 2) Start-up and operation of composite water purification process system a) The place to be treated is a shrimp breeding pond in the Yellow River Delta region. The water in the breeding pond is used as the water of the in-situ odor removal and nitrogen removal system (internal circulation system) of the breeding pond; after the internal circulation treatment, the water in the breeding pond flows out from the outlet of the breeding pond and enters the external tail water treatment pool, which is used as the external tail water odor removal and nitrogen removal reuse system (external circulation system).

[0056] Among them, the water quality indicators in the internal circulation system are as follows: COD concentration 60mg / L, nitrate concentration 22mg / L, nitrite concentration 0.2mg / L, ammonia nitrogen concentration 1.5mg / L, and 2-methylisoborneol concentration 122ng / L.

[0057] b) Add internal circulation deodorizing and denitrifying bacteria to the in-situ deodorizing and denitrifying system (internal circulation system) of the aquaculture pond, and the inoculation amount is 3.0g / m 2 .

[0058] The in-situ deodorization and denitrification system (internal circulation system) of the aquaculture pond is equipped with an intelligent feeding control system to accurately control the amount of additional organic carbon source added to 5% of the feeding amount, stimulating the biological denitrification of the bacterial agent and the production of glutamate. The organic carbon sources include brown sugar, bagasse, wheat bran, and rice bran; and an intelligent temperature, dissolved oxygen, and pH control system is installed to control the dissolved oxygen of the system to not be lower than 5mg / L; the pH value is not lower than 6.5 and not higher than 8.5, ensuring that the internal circulation system is in the appropriate environment for the growth of aquaculture organisms and bacterial agent water purification (see Figure 2 ).

[0059] After the above-mentioned in-situ treatment, the water quality of the aquaculture pond was tested, and the concentrations of COD, nitrate, nitrite, and ammonia nitrogen were 30, 18, 0.1, and 0.88 mg / L, respectively, and the concentration of 2-methylisoborneol was 50 ng / L.

[0060] d) The external tail water deodorization and denitrification recycling system (external circulation system) is equipped with fillers (see Figure 2 ), and inoculate the external circulation deodorizing and denitrifying bacteria on the filler, the inoculation amount of the bacteria is 0.2g / m 2. An electrocoagulation-microfiltration device is installed at the front end of the external tail water deodorization and denitrification reuse system (external circulation system), and an ozone or ultraviolet disinfection system is installed at the end; at the same time, a dissolved oxygen and pH intelligent control system are installed. When the dissolved oxygen in the tail water is higher than 5mg / L, aeration is stopped, and the pH value is not lower than 6.0 and not higher than 9.0. Ensure that the external circulation system is in the suitable environment range for bacterial agent water purification.

[0061] After external circulation treatment, the effluent COD concentration is 5.9mg / L, nitrate concentration is 3.9mg / L, nitrite concentration is 0.04mg / L, ammonia nitrogen concentration is 0.12mg / L, and 2-methylisoborneol concentration is 10.5ng / L. The removal rates of various pollutants are COD≥90%, ammonia nitrogen≥90%, nitrite≥80%, nitrate≥80%, and 2-methylisoborneol≥90%. The treated tail water is returned to the aquaculture pond for recycling.

[0062] e) The aquaculture tail water is treated in situ by the aquaculture pond in-situ odor removal and denitrification system (internal circulation system). After the in-situ treatment, part of the tail water is diverted to the external tail water odor removal and denitrification reuse system (external circulation system) for further purification and treatment, and finally flows back to the aquaculture pond. Example 5

[0063] Deep treatment and recycling of odor removal and nitrogen removal of aquaculture water in a grouper aquaculture process in coastal areas: 1) Preparation method of the deodorizing and denitrifying bacterial agent a) Strain propagation: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1, and nitrifying bacteria were inoculated into the propagation medium at an inoculation amount of 10wt%, and cultured at 20°C for 72 hours. After the culture time, the bacteria were collected. The culture medium for strain propagation is glutamic acid (0.2%), glucose (0.2%), and sucrose (1%) by mass percentage, and the balance is water.

[0064] b) Preparation of mixed bacterial powder: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 and nitrifying bacteria obtained by propagation were freeze-dried separately to obtain single strain dry powder of each strain.

[0065] The dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of Enterococcus sp.YTLJ-N-SXH1 were assembled and mixed in a ratio of 5:1 to prepare an internal circulation type odor removal and denitrification mixed bacterial powder; the dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of nitrifying bacteria were assembled and mixed in a ratio of 6:1 to prepare an external circulation type odor removal and denitrification mixed bacterial powder.

[0066] c) Preparation of auxiliary agent: Take the residual bait of farmed feces (obtained from farmed grouper in this embodiment) and mix it with wheat bran, soybean meal and bagasse after disinfection with potassium bisulfate in a conventional manner. The mass mixing ratio of feces residual bait, wheat bran, soybean meal and bagasse is 1:1:1:1. After mixing, add 0.6% (mass) of expanded Glutamicibacter sp. YTLJ-N-S7, 0.3% (mass) of NH4NO3 and glutamic acid mixture, control the water content of the reaction system to 60% after mixing, and then ferment. After the fermentation is completed, collect the fermented product, dry it, add 0.8% polyglutamic acid and mix to obtain an auxiliary agent. Among them, NH4NO3 and glutamic acid in the mixture are mixed in a mass ratio of 1:2.

[0067] d) Preparation of bacterial agent: The internal circulation deodorizing and denitrifying mixed bacterial powder or the external circulation deodorizing and denitrifying mixed bacterial powder obtained in step b is mixed with the auxiliary agent obtained in step c at a ratio of 1:1 to 100 (mass ratio) to prepare an internal circulation deodorizing and denitrifying bacterial agent for aquaculture ponds and an external circulation deodorizing and denitrifying bacterial agent suitable for an external water treatment system of aquaculture ponds.

[0068] 2) Start-up and operation of composite water purification process system a) The place to be treated is a grouper breeding pond in a coastal area. The water in the breeding pond is used as the water of the in-situ odor removal and denitrification system (internal circulation system) of the breeding pond; after the internal circulation treatment, the water in the breeding pond flows out from the outlet of the breeding pond and enters the external tail water treatment pool, which is used as the external tail water odor removal and denitrification reuse system (external circulation system).

[0069] Among them, the water quality indicators in the internal circulation system are as follows: COD concentration 82mg / L, nitrate concentration 17mg / L, nitrite concentration 0.5mg / L, ammonia nitrogen concentration 1.2mg / L, and geosmin concentration 137ng / L.

[0070] b) Add internal circulation deodorizing and denitrifying bacteria to the in-situ deodorizing and denitrifying system (internal circulation system) of the aquaculture pond. The inoculation amount of bacteria is 5.0g / m 2 .

[0071] c) The in-situ odor removal and denitrification system (internal circulation system) of the aquaculture pond is equipped with an intelligent feeding control system to accurately control the amount of additional organic carbon source added to 10% of the feeding amount, stimulating the biological denitrification of the bacterial agent and the production of glutamate. The organic carbon source includes sodium acetate, sodium butyrate, white sugar, and brown sugar; and an intelligent temperature, dissolved oxygen and pH control system is installed to control the dissolved oxygen of the system to not less than 5mg / L; the pH value is not less than 6.5 and not higher than 8.5, to ensure that the internal circulation system is in the appropriate environment for the growth of aquaculture organisms and bacterial agent water purification (see Figure 2 ).

[0072] After the above-mentioned in-situ treatment, the water quality of the aquaculture pond was tested, and the concentrations of COD, nitrate, nitrite, and ammonia nitrogen were 35, 11, 0.1, and 0.6 mg / L, respectively, and the concentration of geosmin in the tail water was 56 ng / L.

[0073] d) The external tail water deodorization and denitrification recycling system (external circulation system) is equipped with fillers (see Figure 2 ), and inoculate the external circulation deodorizing and denitrifying bacteria on the filler, the inoculation amount of the bacteria is 0.5g / m 2 . The external tail water deodorization and denitrification reuse system (external circulation system) is equipped with an electric flocculation-microfiltration device at the front end and an ozone or ultraviolet disinfection system at the end; at the same time, a dissolved oxygen and pH intelligent control system are installed. Aeration is stopped when the dissolved oxygen in the tail water is higher than 5 mg / L, and the pH value is not lower than 6.0 and not higher than 9.0. Ensure that the external circulation system is in the suitable environment range for bacterial agent water purification.

[0074] After external circulation treatment, the effluent COD, nitrate, nitrite, and ammonia nitrogen concentrations are 8.0, 3.3, 0.1, and 0.1 mg / L, respectively, and the geosmin concentration is 35 ng / L. The pollutant removal rates in the tail water reach COD ≥ 90%, ammonia nitrogen ≥ 90%, nitrite ≥ 80%, nitrate ≥ 80%, and geosmin ≥ 70%, respectively. The treated tail water is returned to the aquaculture pond for recycling.

[0075] e) The aquaculture tail water is treated in situ by the aquaculture pond in-situ odor removal and denitrification system (internal circulation system). After the in-situ treatment, part of the tail water is diverted to the external tail water odor removal and denitrification reuse system (external circulation system) for further purification and treatment, and finally flows back to the aquaculture pond. Example 6

[0076] Deep treatment and recycling of aquaculture water for deodorization and nitrogen removal during industrial aquaculture of crucian carp: 1) Preparation method of deodorizing and denitrifying bacterial agent a) Strain propagation: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1, and nitrifying bacteria were inoculated into the propagation medium at an inoculation amount of 10wt%, and cultured at 20°C for 72 hours. After the culture time, the bacteria were collected. The culture medium for strain propagation is glutamic acid (0.2%), glucose (0.2%), and sucrose (1%) by mass percentage, and the balance is water.

[0077] b) Preparation of mixed bacterial powder: Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 and nitrifying bacteria obtained by propagation were freeze-dried separately to obtain single strain dry powder of each strain.

[0078] The dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of Enterococcus sp.YTLJ-N-SXH1 were assembled and mixed in a ratio of 10:1 to prepare an internal circulation type odor removal and denitrification mixed bacterial powder; the dry powder of Glutamicibacter sp. YTLJ-N-S7 and the dry powder of nitrifying bacteria were assembled and mixed in a ratio of 10:1 to prepare an external circulation type odor removal and denitrification mixed bacterial powder.

[0079] c) Preparation of auxiliary agent: Take the residual bait of farmed feces (obtained from farmed crucian carp in this embodiment) and mix it with wheat bran, soybean meal and bagasse after disinfection with potassium bisulfate in a conventional manner. The mass mixing ratio of the residual bait of feces, wheat bran, soybean meal and bagasse is 1:1:1:1. After mixing, add 1.0% (mass) of expanded Glutamicibacter sp. YTLJ-N-S7, 0.5% (mass) of NH4NO3 and glutamic acid mixture, control the water content of the reaction system to 80% after mixing, and then ferment. After the fermentation is completed, collect the fermented product, dry it, add 1.0% polyglutamic acid and mix to obtain an auxiliary agent. Among them, NH4NO3 and glutamic acid in the mixture are mixed in a mass ratio of 1:5.

[0080] d) Preparation of bacterial agent: The internal circulation deodorizing and denitrifying mixed bacterial powder or the external circulation deodorizing and denitrifying mixed bacterial powder obtained in step b is mixed with the auxiliary agent obtained in step c at a ratio of 1:100 (mass ratio) to prepare an internal circulation deodorizing and denitrifying bacterial agent for aquaculture ponds and an external circulation deodorizing and denitrifying bacterial agent suitable for an external water treatment system of aquaculture ponds.

[0081] 2) Start-up and operation of composite water purification process system a) The place to be treated is a crucian carp factory breeding pond, and the water in the breeding pond is used as the water of the breeding pond in-situ odor removal and denitrification system (internal circulation system); after the internal circulation treatment in the breeding pond, the water flows out from the outlet of the breeding pond and enters the external tail water treatment pool, which is used as the external tail water odor removal and denitrification reuse system (external circulation system).

[0082] Among them, the water quality indicators in the internal circulation system are as follows: COD concentration 73mg / L, nitrate concentration 20mg / L, nitrite concentration 0.4mg / L, ammonia nitrogen concentration 1.4mg / L, and geosmin concentration 165ng / L.

[0083] b) Add internal circulation deodorizing and denitrifying bacteria to the in-situ deodorizing and denitrifying system (internal circulation system) of the aquaculture pond. The inoculation amount of bacteria is 10.0g / m 2 .

[0084] c) The in-situ odor removal and denitrification system (internal circulation system) of the aquaculture pond is equipped with an intelligent feeding control system to accurately control the amount of additional organic carbon source added to 1% of the feeding amount, stimulating the biological denitrification of the bacterial agent and the production of glutamate. The organic carbon sources include sodium acetate, sodium propionate, sodium butyrate, white sugar, and brown sugar; and an intelligent temperature, dissolved oxygen and pH control system is installed to control the dissolved oxygen of the system to not be less than 5mg / L; the pH value is not less than 6.5 and not higher than 8.5, ensuring that the internal circulation system is in the appropriate environment for the growth of aquaculture organisms and the purification of water by bacterial agents (see Figure 2 ).

[0085] After the above in-situ treatment, the water quality of the breeding pond was tested, and the COD concentration was 40 mg / L, the nitrate concentration was 8.4 mg / L, the nitrite concentration was 0.4 mg / L, the ammonia nitrogen concentration was 0.7 mg / L, and the geosmin concentration was 85 ng / L.

[0086] d) The external tail water deodorization and denitrification recycling system (external circulation system) is equipped with fillers (see Figure 2 ), and inoculate the external circulation deodorizing and denitrifying bacteria on the filler, the inoculation amount of the bacteria is 1.0g / m 2 . The external tail water deodorization and denitrification reuse system (external circulation system) is equipped with an electric flocculation-microfiltration device at the front end and an ozone or ultraviolet disinfection system at the end; at the same time, a dissolved oxygen and pH intelligent control system are installed. Aeration is stopped when the dissolved oxygen in the tail water is higher than 5 mg / L, and the pH value is not lower than 6.0 and not higher than 9.0. Ensure that the external circulation system is in the suitable environment range for bacterial agent water purification.

[0087] After external circulation treatment, the effluent COD concentration is 8.0mg / L, nitrate concentration is 2.7mg / L, nitrite concentration is 0.09mg / L, ammonia nitrogen concentration is 0.08mg / L, and geosmin concentration is 15ng / L. The pollutant removal rates of the composite system are COD ≥ 90%, ammonia nitrogen ≥ 90%, nitrite ≥ 80%, nitrate ≥ 80%, and geosmin ≥ 90%. The treated tail water is returned to the aquaculture pond for recycling.

[0088] e) The aquaculture tail water is treated in situ by the aquaculture pond in-situ odor removal and denitrification system (internal circulation system). After the in-situ treatment, part of the tail water is diverted to the external tail water odor removal and denitrification reuse system (external circulation system) for further purification and treatment, and finally flows back to the aquaculture pond.

Claims

1. A deodorizing and denitrifying bacteria, characterized in that: The deodorizing and denitrifying bacteria is Glutamicibacter sp. YTLJ-N-S7, which was deposited in the Guangdong Provincial Microbiological Culture Collection Center on May 26, 2021, with the collection number GDMCC No: 61694, the classification name is Glutamicibacter sp., and the collection address is Guangzhou, China.

2. An application of the deodorizing and denitrifying bacteria according to claim 1, characterized in that: The application of Glutamicibacter sp. YTLJ-N-S7 in water purification, deodorization and denitrification.

3. A water purification, deodorization and denitrification bacterial agent, characterized in that: The bacterial agent contains the Glutamicibacter sp. YTLJ-N-S7 described in claim 1.

4. The water purification, deodorization and denitrification bacterial agent according to claim 3, characterized in that: The bacterial agent is one or more of Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1 or nitrifying bacteria. The Enterococcus sp. YTLJ-N-SXH1 was deposited in the Guangdong Provincial Microbiological Culture Collection Center on November 26, 2021, with the collection number GDMCC No: 62080, and the classification name is Enterococcus sp., and the collection address is Guangzhou, China.

5. The water purification, deodorization and denitrification bacterial agent according to claim 4, characterized in that: The Glutamicibacter sp. YTLJ-N-S7, Enterococcus sp. YTLJ-N-SXH1, and nitrifying bacteria are propagated separately, dried to obtain dry powders of the strains, and then the propagated Glutamicibacter sp. YTLJ-N-S7 dry powder and Enterococcus sp. YTLJ-N-SXH1 dry powder are mixed at a mass ratio of 1 to 10:1 to obtain bacterial powder A; Glutamicibacter sp. YTLJ-N-S7 dry powder and nitrifying bacteria dry powder are mixed in a mass ratio of 1 to 10:1 to obtain bacterial powder B; The above-mentioned bacterial powders were mixed with the auxiliary agents at a mass ratio of 1:1 to 100 to obtain the bacterial agents, and the total effective viable count of each bacterial agent was ≥1.0×10 9 Pieces / mL; Alternatively, the bacterial powders are mixed with the auxiliary agents respectively, and the obtained mixtures are mixed again to obtain the bacterial agent.

6. The water purification, deodorization and denitrification bacterial agent according to claim 5, characterized in that: The auxiliary agent is a mixture of detoxified livestock manure bait with wheat bran, soybean meal and bagasse in a mass ratio of 1:1:1:1; after mixing, 0.1% to 1.0% by mass of expanded Glutamicibacter sp. YTLJ-N-S7 dry powder and 0.05% to 0.5% by mass of mixture A are added, and after mixing, the water content of the reaction system is controlled to be 40% to 80%, and then fermentation is carried out, the fermented product is collected and 0.5-1.0% by mass of polyglutamic acid is added to mix to prepare the auxiliary agent; wherein the mixture A is a mixture of NH4NO3 and glutamic acid in a mass ratio of 1:1 to 5.

7. A use of the bacterial agent according to any one of claims 3 to 6, characterized in that: The bacterial agent is used in water purification, odor removal and nitrogen removal.

8. The use according to claim 7, characterized in that: The bacterial agent is used in composite intelligent treatment of water inside and outside aquaculture ponds, deodorization, and denitrification.

9. The use according to claim 7, characterized in that: The composite intelligent treatment of water inside and outside the breeding pond refers to using the bacterial agent obtained by mixing bacterial powder A with an auxiliary agent to treat the in-situ internal circulating water of the breeding pond; and using the bacterial agent obtained by mixing bacterial powder B with an auxiliary agent to treat the external circulating tail water outside the breeding pond.

10. The use according to claim 9, characterized in that: The inoculation amount of the bacterial agent in the treatment, deodorization and denitrification of the in-situ circulating water in the aquaculture pond is 1.0 to 10.0 g / m 2 The inoculation amount of the bacterial agent in the treatment, deodorization and denitrification of the external circulating tail water of the aquaculture pond is 0.1-1.0 g / m 2 .

Images