Clean utilization method of VC (Vitamin C) film slurry

Abstract

The invention discloses a VC (Vitamin C) membrane slurry clean utilization method which comprises the following steps: filtering VC membrane slurry through a ceramic membrane, hydrolyzing a concentrated solution obtained by filtering through protease, flocculating by adding a flocculating agent, separating, drying and crushing an obtained solid substance, and applying the solid substance into a tetracycline fermentation culture medium to replace part of yeast powder and corn steep liquor. Nutrient substances in the VC film slurry are recycled, waste is turned into wealth, on one hand, the antibiotic production cost is reduced, on the other hand, the pressure of environmental protection treatment is reduced, the production environment can be effectively improved, and clean production of the VC film slurry is achieved.

Description

Technical Field

[0001] The invention relates to the field of biology and new medicine technology, and in particular to a method for cleaning and utilizing VC membrane slurry. Background Art

[0002] The existing VC membrane slurry treatment method is to enter the biochemical system, or to make organic fertilizers and feed additives with other raw materials. VC membrane slurry contains rich nutrients, most of which are bacterial proteins. If it is not treated in time, it will produce a strong odor and affect the ecological environment of the enterprise plant. If it is treated, it will require additional investment and increase production costs. Therefore, it is particularly important to study a method that can treat VC membrane at a low cost. Summary of the invention

[0003] The purpose of the present application is to provide a method for cleanly utilizing VC membrane slurry, in which the protein in the membrane slurry replaces part or all of the yeast powder and corn slurry in the fermentation medium, so as to reduce the fermentation cost of tetracycline, turn VC membrane slurry into treasure, and reduce the processing pressure on the VC membrane slurry.

[0004] In order to solve the above technical problems, the present application provides a VC membrane slurry cleaning and utilization method, comprising:

[0005] Step 1: let the VC membrane slurry settle for 60-90 minutes and remove the white precipitate at the bottom;

[0006] Step 2: Filter the upper separated liquid through a 100 nm ceramic membrane to obtain a concentrated liquid, and control the concentration multiple to 6-8 times and the solid content to 15%-20%;

[0007] Step 3, adjusting the pH of the concentrated solution to 7.0-8.5, heating in a water bath to 40° C.-55° C., and adding 0.1%-0.15% by volume of neutral protease to the concentrated solution for hydrolysis to obtain an enzymatic solution, wherein the hydrolysis time is controlled to be 60 min-90 min;

[0008] Step 4: Add 0.5% chitosan solution to the enzymatic solution for flocculation to obtain a flocculated solution, wherein the chitosan solution is added in a proportion of 5% to 10% of the volume of the enzymatic solution, and stir slowly for 1 min to 2 min;

[0009] Step 5: Use a diaphragm pump to pump the flocculated liquid into a plate and frame or horizontal screw centrifuge for separation, and put the solid separated by the plate and frame or the horizontal screw centrifuge into an oven for drying to obtain a solid material, control the temperature at 60° C.-65° C., and control the moisture at 6%-10%;

[0010] Step 6: Grind the solid matter and apply it to tetracycline fermentation medium to replace part of yeast powder and corn steep liquor.

[0011] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step one, during the sedimentation process of the VC membrane slurry, the sedimentation time is controlled to be 60 minutes. In the step three, the pH of the concentrated solution is adjusted to 8.0, and the water bath heating temperature is controlled to be 45°C. In the step five, the solids separated by the plate and frame or the horizontal screw centrifuge are placed in an oven for drying, and the temperature of the oven is controlled to be 60°C.

[0012] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time of the VC membrane slurry is controlled to be 70 minutes.

[0013] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided, wherein in step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time of the VC membrane slurry is controlled to be 75 minutes.

[0014] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided, wherein in step 4, the time for slowly stirring the mixture of the chitosan solution and the enzymatic hydrolyzate is controlled to be 1.5 min.

[0015] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided, wherein in step three, the pH of the concentrated liquid obtained by filtering the ceramic membrane is adjusted to 8.0.

[0016] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided, wherein in step three, the pH of the concentrated liquid obtained by filtering the ceramic membrane is adjusted to 8.2.

[0017] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided, wherein in step three, the water bath heating temperature of the concentrated liquid obtained by filtering the ceramic membrane is 45°C.

[0018] As a preferred embodiment, a method for cleaning and utilizing VC membrane slurry is provided, wherein in step three, the water bath heating temperature of the concentrated liquid obtained by filtering the ceramic membrane is 48°C.

[0019] Compared with the prior art, the present invention provides a method for cleanly utilizing VC membrane slurry, wherein the VC membrane slurry is filtered through a ceramic membrane, the concentrated solution obtained by filtration is then hydrolyzed by protease, flocculated and separated by adding a flocculant, and the obtained solid matter is dried and crushed, and applied to tetracycline fermentation medium to replace part of yeast powder and corn syrup. The nutrients in the VC membrane slurry are reused, and waste is turned into treasure, which reduces the production cost of antibiotics on the one hand, and reduces the pressure of environmental protection treatment on the other hand, which can effectively improve the production environment and realize the clean production of VC membrane slurry. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solution of the present application, the following briefly introduces the drawings required for use in the embodiments. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without inventiveness.

[0021] Figure 1 A schematic diagram of a VC membrane slurry cleaning and utilization method provided in an embodiment of the present application. DETAILED DESCRIPTION

[0022] In order to enable those skilled in the art to better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings.

[0023] The core of this application is to provide a method for clean utilization of VC membrane slurry, using the protein in the membrane slurry to replace part or all of the yeast powder and corn slurry in the fermentation medium, so as to reduce the fermentation cost of tetracycline, turn VC membrane slurry into treasure, and reduce the processing pressure on VC membrane slurry.

[0024] Figure 1 A schematic diagram of a VC membrane slurry cleaning and utilization method provided in the embodiment of the present application, see Figure 1 shown.

[0025] A method for cleaning and utilizing VC membrane slurry comprises the following steps:

[0026] Step 1: let the VC membrane slurry settle for 60-90 minutes and remove the white precipitate at the bottom;

[0027] Step 2: Filter the upper separated liquid through a 100 nm ceramic membrane to obtain a concentrated liquid, and control the concentration multiple to 6-8 times and the solid content to 15%-20%;

[0028] Step 3, adjusting the pH of the concentrated solution to 7.0-8.5, heating in a water bath to 40° C.-55° C., and adding 0.1%-0.15% by volume of neutral protease to the concentrated solution for hydrolysis to obtain an enzymatic solution, wherein the hydrolysis time is controlled to be 60 min-90 min;

[0029] Step 4: Add 0.5% chitosan solution to the enzymatic solution for flocculation to obtain a flocculated solution, wherein the chitosan solution is added in a proportion of 5% to 10% of the volume of the enzymatic solution, and stir slowly for 1 min to 2 min;

[0030] Step 5: Use a diaphragm pump to pump the flocculated liquid into a plate and frame or horizontal screw centrifuge for separation, and put the solid separated by the plate and frame or the horizontal screw centrifuge into an oven for drying to obtain a solid material, control the temperature at 60° C.-65° C., and control the moisture at 6%-10%;

[0031] Step 6: Grind the solid matter and apply it to tetracycline fermentation medium to replace part of yeast powder and corn steep liquor.

[0032] In the present embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step one, during the sedimentation process of the VC membrane slurry, the sedimentation time is controlled to be 60 minutes. In the step three, the pH of the concentrated solution is adjusted to 8.0, and the water bath heating temperature is controlled to be 45°C. In the step five, during the process of placing the solids separated by the plate and frame or the horizontal screw centrifuge into an oven for drying, the temperature of the oven is controlled to be 60°C.

[0033] In this embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time of the VC membrane slurry is controlled to be 70 minutes.

[0034] In this embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time of the VC membrane slurry is controlled to be 75 minutes.

[0035] In this embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step 4, the time for slowly stirring the mixture of the chitosan solution and the enzymatic hydrolyzate is controlled to be 1.5 min.

[0036] In this embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step three, the pH of the concentrated liquid obtained by filtering the ceramic membrane is adjusted to 8.0.

[0037] In this embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step three, the pH of the concentrated liquid obtained by filtering the ceramic membrane is adjusted to 8.2.

[0038] In the present embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step three, the concentrated liquid obtained by filtering the ceramic membrane is heated in a water bath at a temperature of 45°C.

[0039] In the present embodiment, a method for cleaning and utilizing VC membrane slurry is provided. In the step three, the water bath heating temperature of the concentrated liquid obtained by filtering the ceramic membrane is 48°C.

[0040] In order to enable those skilled in the art to better understand the present solution, the present solution is described in detail below with reference to specific embodiments.

[0041] Example 1

[0042] Take VC membrane slurry, settle for 60min, measure 30L, determine its solid content of 4.5%, use 100nm ceramic membrane filtration, separate 4.2L concentrate, 25.8L dialyzate, concentrate 7.14 times, the solid content of the concentrate is 18.76%, adjust the pH of the concentrate to 8.0 with liquid alkali, heat to 45℃, add 4.2g protease to hydrolyze, hydrolyze for 80min, then add 210ml chitosan solution with a concentration of 0.5%, stir slowly for 1.5min, use a diaphragm pump to filter through a plate and frame, separate 3.11L clear liquid, 1300g wet fungus residue, then put the wet fungus residue into a 60℃ oven, dry it, and the moisture content is 7.84%, apply it to tetracycline fermentation medium, replace part of yeast powder and corn steep liquor, and there is no abnormality in the fermentation process.

[0043] Example 2

[0044] Take VC membrane slurry, settle for 75 minutes, measure 30L, determine its solid content of 4.2%, use 100nm ceramic membrane filtration, separate 4.0L concentrate, 26.0L dialyzate, concentrate 7.5 times, the solid content of the concentrate is 17.85%, adjust the pH of the concentrate to 8.2 with liquid alkali, heat to 48°C, add 5.0g protease to hydrolyze, hydrolyze for 85min, then add 320ml chitosan solution with a concentration of 0.5%, stir slowly for 1.5min, use a diaphragm pump to filter through a plate and frame, separate 3.13L clear liquid, 1190g wet fungus residue, then put the wet fungus residue into a 60°C oven, dry it, and the moisture content is 7.66%, apply it to tetracycline fermentation medium, replace part of yeast powder and corn steep liquor, and there is no abnormality in the fermentation process.

[0045] Example 3

[0046] Take VC membrane slurry, settle for 70 minutes, measure 30L, determine its solid content of 4.68%, use 100nm ceramic membrane filtration, separate 4.11L concentrate, 25.89L dialyzate, concentrated 7.3 times, the solid content of the concentrate is 17.51%, adjust the pH of the concentrate to 8.0 with liquid alkali, heat to 45°C, add 6.17g protease to hydrolyze, hydrolyze for 80min, then add 411ml chitosan solution with a concentration of 0.5%, stir slowly for 1.5min, use a diaphragm pump to filter through a plate and frame, separate 3.32L clear liquid, 1200g wet fungus residue, then put the wet fungus residue into a 60°C oven, dry it, and the moisture content is 8.26%, apply it to tetracycline fermentation medium, replace part of yeast powder and corn steep liquor, and there is no abnormality in the fermentation process.

[0047] Yeast powder detection method

[0048] Moisture% Total nitrogen % Dissolved phosphorus ug / g Case 1 7.84 8.5 7506 Case 2 7.86 8.2 8296 Case 3 8.26 7.8 7684 Sample Control 8.82 6.5 4041 Quality Standards ≤9.0 ≥6.4 ≤12000

[0049] The embodiment of the present application provides a method for cleanly utilizing VC membrane slurry, wherein the VC membrane slurry is filtered through a ceramic membrane, the concentrated solution obtained by filtration is then hydrolyzed by protease, flocculated and separated by adding a flocculant, and the obtained solid matter is dried and crushed, and applied to tetracycline fermentation medium to replace part of yeast powder and corn syrup. The nutrients in the VC membrane slurry are reused, and waste is turned into treasure. On the one hand, the production cost of antibiotics is reduced, and on the other hand, the pressure of environmental protection treatment is reduced, which can effectively improve the production environment and realize the clean production of VC membrane slurry.

[0050] Those skilled in the art will readily appreciate other embodiments of the present application after considering the specification and practicing the application disclosed herein. The present application is intended to cover any variations, uses or adaptations of the present application, which follow the general principles of the present application and include common knowledge or customary technical means in the art disclosed in the present application. The specification and examples are to be regarded as exemplary only, and the true scope of the present application is indicated by the claims.

[0051] It should be understood that the present application is not limited to the precise structure described above and shown in the drawings, and various modifications and changes can be made without departing from its scope. The above-described embodiments of the present application do not constitute a limitation on the scope of protection of the present application.

Claims

1. A method for cleaning and utilizing VC membrane slurry, characterized in that: include: Step 1: let the VC membrane slurry settle for 60-90 minutes and remove the white precipitate at the bottom; Step 2: Filter the upper separated liquid through a 100 nm ceramic membrane to obtain a concentrated liquid, and control the concentration multiple to 6-8 times and the solid content to 15%-20%; Step 3, adjusting the pH of the concentrated solution to 7.0-8.5, heating in a water bath to 40° C.-55° C., and adding 0.1%-0.15% by volume of neutral protease to the concentrated solution for hydrolysis to obtain an enzymatic solution, wherein the hydrolysis time is controlled to be 60 min-90 min; Step 4: Add 0.5% chitosan solution to the enzymatic solution for flocculation to obtain a flocculated solution, wherein the chitosan solution is added in a proportion of 5% to 10% of the volume of the enzymatic solution, and stir slowly for 1 min to 2 min; Step 5: Use a diaphragm pump to pump the flocculated liquid into a plate and frame or horizontal screw centrifuge for separation, and put the solid separated by the plate and frame or the horizontal screw centrifuge into an oven for drying to obtain a solid material, control the temperature at 60° C.-65° C., and control the moisture at 6%-10%; Step 6: Grind the solid matter and apply it to tetracycline fermentation medium to replace part of yeast powder and corn steep liquor.

2. A VC membrane slurry cleaning and utilization method according to claim 1, characterized in that: In the step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time is controlled to be 60 minutes. In the step 3, the pH of the concentrated solution is adjusted to 8.0, and the water bath heating temperature is controlled to be 45°C. In the step 5, the solid separated by the plate and frame or the horizontal screw centrifuge is placed in an oven for drying, and the temperature of the oven is controlled to be 60°C.

3. A VC membrane slurry cleaning and utilization method according to claim 1, characterized in that: In the step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time of the VC membrane slurry is controlled to be 70 minutes.

4. A VC membrane slurry cleaning and utilization method according to claim 1, characterized in that: In the step 1, during the sedimentation process of the VC membrane slurry, the sedimentation time of the VC membrane slurry is controlled to be 75 minutes.

5. The method for cleaning and utilizing VC membrane slurry according to claim 1, characterized in that: In the step 4, the time for slowly stirring the mixture of the chitosan solution and the enzymatic hydrolysate is controlled to be 1.5 min.

6. A VC membrane slurry cleaning and utilization method according to claim 1, characterized in that: In the step three, the pH of the concentrated solution obtained by filtering through the ceramic membrane is adjusted to 8.

0.

7. The method for cleaning and utilizing VC membrane slurry according to claim 1, characterized in that: In the step three, the pH of the concentrated solution obtained by filtering through the ceramic membrane is adjusted to 8.

2.

8. The method for cleaning and utilizing VC membrane slurry according to claim 1, characterized in that: In the step 3, the water bath heating temperature of the concentrated solution obtained by filtering the ceramic membrane is 45°C.

9. The method for cleaning and utilizing VC membrane slurry according to claim 1, characterized in that: In the step 3, the water bath heating temperature of the concentrated solution obtained by filtering the ceramic membrane is 48°C.

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