Device for detecting antibiotic content of livestock wastewater and use method thereof

Abstract

The invention relates to the technical field of environment detection, in particular to a device for detecting the content of antibiotics in livestock wastewater and a use method of the device. The device comprises a detection plate, a container is installed at a hole position of the detection plate, a micro-flow control pipeline inputs liquid into the container, and the liquid in the container is pumped out through a liquid outlet channel; the detection device can detect the antibiotic content in the livestock and poultry wastewater. Aiming at the defects of detection lag and inconvenience in operation when an existing liquid chromatography-mass spectrometry detection instrument is used for detecting the antibiotic content of the livestock wastewater, the device provided by the invention can be used for simply, conveniently and quickly detecting whether the antibiotic in the livestock wastewater meets the emission standard or not, and is small in size and convenient to operate. The device is suitable for being mounted at a wastewater discharge end of a livestock farm for in-situ detection, and can realize real-time synchronous detection of various antibiotics in livestock wastewater. Besides, the detection plate can be used for many times, the dosage of used chemicals is small, the detection cost is remarkably reduced, and meanwhile, the automation degree of the device is higher by using a micro-flow control pipeline.

Description

Technical Field

[0001] The invention relates to the technical field of environmental detection, and in particular to a device for detecting the content of antibiotics in livestock wastewater and a method for using the device. Background Art

[0002] The misuse of antibiotics can harm human health, and the resistance genes produced can cause immeasurable damage to both the human body and the natural world. Due to the irrational and illegal use of drugs, the pollution caused by the continuous discharge of antibiotics in livestock and poultry farming into the environment has attracted widespread attention at home and abroad.

[0003] For example, Chinese invention patent publication number CN114965777B discloses an ultra-high performance liquid chromatography-tandem mass spectrometry method for detecting quinolone antibiotics in sediments, comprising the following steps: (1) sample preparation; (2) ultrasonic extraction; (3) concentration and dilution; (4) fully automatic solid phase extraction; (5) concentration and volume determination; (6) ultra-high performance liquid chromatography-tandem mass spectrometry detection; (7) drawing of a standard curve; and (8) sample analysis.

[0004] Another example is a Chinese invention patent with publication number CN114345430B, which discloses a portable device for simultaneously detecting multiple antibiotic residues through a paper-based microfluidic chip, which includes a cover plate, a paper-based microfluidic chip, a reaction well and a tray; the paper-based microfluidic chip is arranged between the cover plate and the tray; the center of the cover plate is provided with a reaction well; the bottom of the reaction well is arranged above the paper-based microfluidic chip; the cover plate, the paper-based microfluidic chip and the tray are of the same shape. The invention also provides an indirect competitive immunoassay method for detecting multiple antibiotics using the device, which can perform qualitative and quantitative analysis of different antibiotics based on the fitting curve by analyzing and processing the signal intensity of the reaction area.

[0005] For wastewater discharged from livestock farms, it is necessary to detect whether the antibiotics in the wastewater meet the discharge requirements at the discharge end. However, the LC-MS detection instrument used in the above detection method is large in size, expensive, and has a slow detection speed. Therefore, this type of LC-MS detection instrument is not suitable for installation at the discharge end of livestock farm wastewater, and it is also impossible to quickly determine whether the antibiotics in the livestock wastewater meet the discharge requirements.

[0006] Therefore, there is an urgent need to develop simple and rapid methods for detecting antibiotics in livestock wastewater to meet the needs of monitoring antibiotics in livestock wastewater. Summary of the invention

[0007] To solve the above problems, the object of the present invention is to provide a device for detecting the antibiotic content in livestock wastewater and a method for using the same.

[0008] The technical solution provided by the present invention is:

[0009] In a first aspect, a device for detecting the antibiotic content in livestock wastewater comprises a housing, wherein a detection plate is installed in the housing;

[0010] The detection plate is provided with a plurality of holes;

[0011] The detection plate is provided with a liquid inlet channel and a liquid outlet channel connected to the hole position;

[0012] A container is installed in the hole, and the container is provided with an inlet and an outlet which can be connected with the liquid inlet channel and the liquid outlet channel respectively;

[0013] The bottom layer of the inner wall of the container is provided with a capture layer;

[0014] It also includes a microfluidic pipeline connected to the liquid inlet channel and the liquid outlet channel, and the microfluidic pipeline is used to input liquid into the container through the liquid inlet channel and to extract the liquid in the container through the liquid outlet channel;

[0015] The device also comprises a detection module, which is used for detecting the antibiotic content of the liquid in the container or the liquid extracted from the container.

[0016] As an optional technical solution of the first aspect, the detection board includes a first board, a second board, and a third board stacked in sequence;

[0017] The adjacent surfaces of the first plate and the second plate form the liquid inlet channel; the adjacent surfaces of the second plate and the third plate form the liquid outlet channel.

[0018] As an optional technical solution of the first aspect, a card slot is provided on the side wall of the hole; a card strip is provided on the outer wall of the container; when the container is placed in the hole and the card strip is inserted in the card slot, the outlet is aligned with the liquid outlet channel and the inlet is aligned with the liquid inlet channel.

[0019] As an optional technical solution of the first aspect, the microfluidic pipeline includes a plurality of liquid inlet pipelines, which are connected to the liquid inlet channel; a first valve, a first peristaltic pump, and a second valve are installed in sequence along the liquid flow direction of the liquid inlet pipeline; the microfluidic pipeline also includes a liquid outlet pipeline, which is connected to the liquid outlet channel; a third valve and a second peristaltic pump are installed in sequence along the liquid flow direction of the liquid outlet pipeline.

[0020] Optionally, the light source and the photoelectric detection module of the detection module are respectively located on both sides of the liquid outlet pipeline and are arranged correspondingly.

[0021] As an optional technical solution of the first aspect, the detection plate and the container are both made of light-transmitting materials; the holes on the detection plate are arranged in a ring shape; the detection plate is connected to a detection plate driving mechanism, and the detection plate driving mechanism is used to drive the detection plate to rotate step by step between eight stations;

[0022] The eight workstations include the wastewater inlet station, the first washing station, the secondary antibody addition station, the second washing station, the color developer addition station, the incubation color development station, the absorbance detection station, and the container replacement station;

[0023] The wastewater inlet station, the first washing station, the secondary antibody addition station, the second washing station, and the developer addition station are all provided with a pin drive mechanism, which is used to drive a pair of pins to be inserted into or out of the liquid inlet channel, the inlet, and the liquid outlet channel, the outlet respectively;

[0024] The pin corresponding to the inlet is connected to the liquid inlet pipeline through the connecting pipe;

[0025] In the first washing station and the second washing station, the pins corresponding to the outlets are connected to the liquid outlet pipeline through the connecting pipe;

[0026] In the wastewater inlet station, the secondary antibody addition station, and the color developer addition station, plugs are installed in the pins corresponding to the outlets;

[0027] The light source and the photoelectric detection module of the detection module are arranged at the absorbance detection station, and are respectively located on both sides of the hole in the radial direction and are arranged correspondingly.

[0028] As an optional technical solution of the first aspect, the hole is set through the detection plate; there are multiple containers, and the multiple containers are stacked and fixedly connected up and down; and it also includes a container driving mechanism, which is used to drive the containers to move axially along the hole one by one.

[0029] As an optional technical solution of the first aspect, it also includes a solid phase extraction module, which is used to concentrate antibiotics in wastewater, and the microfluidic pipeline is used to input the concentrated antibiotics into the container through the liquid inlet channel.

[0030] In a second aspect, a method for using the device for detecting the antibiotic content in livestock wastewater as described in the first aspect or any optional technical solution of the first aspect, comprising the following steps:

[0031] Place the container in the hole, and connect the inlet and outlet of the container to the liquid inlet channel and the liquid outlet channel respectively;

[0032] The livestock and poultry wastewater is filtered, the filtered wastewater is input into a container and washed, and then a labeled secondary antibody solution is added to the container, which is then washed again, and then a color developer is added to the container. After color development, the detection module is used for detection to determine the antibiotic concentration in the livestock and poultry wastewater.

[0033] As an optional technical solution of the second aspect, before placing the container at the hole position, the bottom layer of the inner wall of the container is coated, washed and sealed to form a capture layer.

[0034] Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects:

[0035] The present invention sets a hole on the detection plate, and sets an independent container to be installed in the hole, and the bottom layer of the inner wall of the container is provided with a capture layer. The livestock wastewater to be detected is pumped into the container through a microfluidic pipeline. In addition, wastewater, cleaning solution, color developer, and secondary antibody solution can also be pumped into the container through the microfluidic pipeline. Then, the detection module is used to detect whether the antibiotic content meets the emission standards. The detection device proposed in this application can easily and quickly detect whether the antibiotics in livestock wastewater meet the emission standards, and the detection device is small in size and is suitable for installation at the wastewater discharge end of the farm for in-situ detection.

[0036] The same detection plate of the present application can be equipped with containers with different capture layers, so that multiple antibiotics in livestock wastewater can be detected in real time and simultaneously.

[0037] In addition, after the container is used, it can be removed from the detection plate, and a new container can be replaced later without replacing the detection plate, and the amount of medicine used is small, which significantly reduces the detection cost. The present invention also proposes a container group formed by stacking multiple containers up and down and fixedly connected, and the containers are driven one by one along the axial direction of the hole by a container driving mechanism. When the current container is used, the next container can be replaced by the container driving mechanism, and there is no need to take out the detection plate to replace the container, so there is no need to interrupt the detection process at other holes of the detection plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a schematic diagram of a detection board in one embodiment of the present application;

[0039] Figure 2 This is a schematic diagram of a first plate, a second plate, and a third plate stacked in one embodiment of the present application;

[0040] Figure 3 This is a schematic diagram of an embodiment of the present application in which the second plate is provided with a liquid inlet channel and a liquid outlet channel;

[0041] Figure 4 This is a cross-sectional view of a first plate, a second plate, and a third plate stacked in one embodiment of the present application;

[0042] Figure 5 This is a schematic diagram of a container in one embodiment of the present application;

[0043] Figure 6 This is a cross-sectional view of a container in one embodiment of the present application;

[0044] Figure 7 This is a cross-sectional view of a container placed in a hole position in one embodiment of the present application;

[0045] Figure 8This is a schematic diagram of a microfluidic pipeline in one embodiment of the present application;

[0046] Fig. 9 This is a schematic diagram of a detection board in another embodiment of the present application;

[0047] Fig.10 This is a schematic diagram of containers stacked and fixedly connected in an embodiment of the present application;

[0048] Fig.11 for Fig.10 Enlarged view of point A in the middle;

[0049] Fig.12 This is a cross-sectional view of a container in another embodiment of the present application.

[0050] Explanation of the symbols in the schematic diagram:

[0051] Detection plate 201, first plate 201-1, second plate 201-2, third plate 201-3, liquid inlet channel 202, liquid outlet channel 203, hole 204, card slot 205, detection plate vent 206;

[0052] Container 301, inlet 302, card strip 303, capture layer 304, outlet 305, container drive mechanism 306, container vent 307;

[0053] A first valve 401, a first peristaltic pump 402, a second valve 403, a third valve 404, a second peristaltic pump 405, a light source 406-1, and a photoelectric detection module 406-2;

[0054] Pin drive mechanism 501, pin 502, connecting tube 503, plug 504;

[0055] Detection plate driving mechanism 601. DETAILED DESCRIPTION

[0056] In order to further understand the content of the present invention, the present invention is described in detail in conjunction with the accompanying drawings and embodiments.

[0057] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change of the proportion relationship or adjustment of the size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical content disclosed by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and so on quoted in this specification are only for the convenience of description, and are not used to limit the scope of implementation. The change or adjustment of their relative relationship should also be regarded as the scope of implementation of the present invention without substantially changing the technical content.

[0058] Embodiment 1

[0059] In this embodiment, the present application proposes a device for detecting the antibiotic content in livestock wastewater, including a housing, in which a detection plate 201, a microfluidic pipeline, and a detection module are installed. The number of holes 204 on the detection plate 201 can be various, such as Figure 1 In the embodiment, the number of the holes 204 is 9. The detection plate 201 can be made of polystyrene, which is relatively mature in the prior art and will not be described in detail here.

[0060] In this application, if Figure 1 As shown, the detection plate 201 is provided with a liquid inlet channel 202 and a liquid outlet channel 203 which are connected to the hole 204. Figure 2-4 As shown, the detection board 201 includes a first board 201-1, a second board 201-2, and a third board 201-3 stacked in sequence, the adjacent surfaces of the first board 201-1 and the second board 201-2 form a liquid inlet channel 202, and the adjacent surfaces of the second board 201-2 and the third board 201-3 form the liquid outlet channel 203. The detection board 201 is made by stacking three boards, and then the channels can be processed on the end surfaces of the first board 201-1, the second board 201-2, and the third board 201-3, and then the corresponding sealing parts are installed, and finally the three boards are fixed together by means such as bonding, etc., and it is more convenient to make the detection board 201.

[0061] In the present application, a container 301 is installed in the hole 204, and a capture layer 304 is provided on the bottom of the inner wall of the container 301. At this time, the detection plate 201 plays the role of supporting the container 301 and providing a liquid inlet channel 202 and a liquid outlet channel 203. In order to allow the liquid in the liquid inlet channel 202 to enter the container 301 after the container 301 is placed in the hole 204, and the liquid in the container 301 can also flow out through the liquid outlet channel 203, an inlet 302 and an outlet 305 that can be connected to the liquid inlet channel 202 and the liquid outlet channel 203 respectively are provided on the container 301.

[0062] The container 301 may be substantially barrel-shaped, with an open opening at the upper end serving as an inlet 302 and an outlet 305 disposed on a side wall. Figure 5-7 The upper end of the container 301 may also be closed, in which case the inlet 302 and the outlet 305 may be disposed on the side wall, as shown. Figure 10-12 shown.

[0063] On the same detection board 201, the user can arrange a variety of containers 301 with different capture layers 304 according to the usage scenario. Then, when detecting whether there are four antibiotics in livestock wastewater, four containers 301 can be arranged on the same detection board 201. The difference between these four containers 301 is that the capture layer 304 is different, that is, each container 301 is used to detect the corresponding antibiotic. Since containers 301 with different capture layers 304 can be arranged as needed on the same detection board 201, the same detection device can simultaneously detect different types of antibiotics in livestock wastewater. In addition, after the container 301 is used, it can be removed from the detection board 201, and a new container 301 can be replaced later without replacing the detection board 201, thereby reducing the cost of use.

[0064] The liquid inlet channel 202 and the liquid outlet channel 203 are connected to the microfluidic pipeline, and the microfluidic pipeline can input liquid, such as cleaning liquid, color developer, secondary antibody solution, livestock wastewater, etc., into the container 301 through the liquid inlet channel 202, and extract the liquid in the container 301 through the liquid outlet channel 203. A detection module is also included, and the detection module can detect the antibiotic content of the liquid in the container 301, or detect the antibiotic content of the liquid extracted from the container 301.

[0065] The detection module includes a light source 406-1 and a photoelectric detection module 406-2, which are arranged on opposite sides of the liquid to be detected. The antibiotic content in the liquid to be detected can be determined by photoelectric detection. The specific technology of photoelectric detection is relatively mature in the prior art and will not be described here.

[0066] When detecting the concentration of antibiotics in livestock and poultry wastewater, the livestock and poultry wastewater is first filtered through a filter device provided in the front housing, and an aqueous filter membrane is used for filtering. The filtered wastewater is added to 100 microliters in the container 301 through a microfluidic pipeline, and incubated at room temperature for 10 minutes. Then the container 301 is cleaned, specifically, 200 microliters of washing liquid are added to the microfluidic pipeline, and washing liquid is added again after the washing liquid is drawn out, and washing liquid is added again after the washing liquid is drawn out, and the cycle is repeated three times to remove unbound antibiotics. Subsequently, a labeled secondary antibody solution is added through the microfluidic pipeline, incubated, and then cleaned again, in order to remove unbound secondary antibodies. Subsequently, 150 microliters of color developing solution are added, and after color development, the detection module is used to measure and judge the concentration of antibiotics in the livestock and poultry wastewater.

[0067] The types of antibiotics detected mainly include quinolone antibiotics, sulfonamide antibiotics, tetracycline antibiotics, macrolide antibiotics and other commonly used veterinary antibiotics. Based on the specific types of antibiotics used in the breeding site, the container 301 can be customized by coating, sealing and other processes. Based on the independence of each container 301, multiple different antibiotics can be detected at the same time.

[0068] Before using this device for testing, it is necessary to test the standard sample and fit the standard curve. Then, the wastewater test results are compared with this standard curve to determine the concentration of antibiotics in livestock and poultry wastewater.

[0069] A sampling bottle can also be placed in the shell. The microfluidic pipeline directly draws samples from the sampling bottle and then tests them after passing through the filtering device, so as to realize the full-process automated detection of livestock and poultry wastewater.

[0070] like Figure 8-9 As shown, the microfluidic pipeline includes a plurality of liquid inlet pipelines, which are connected to the liquid inlet channel 202, and are also connected to livestock wastewater and storage equipment for storing liquids such as cleaning liquid, color developer, and secondary antibody solution. A first valve 401, a first peristaltic pump 402, and a second valve 403 are sequentially installed along the liquid flow direction of the liquid inlet pipeline. When the first valve 401 and the second valve 403 are controlled to be in a connected state, the liquid can be quantitatively extracted into the container 301 through the first peristaltic pump 402.

[0071] The microfluidic pipeline also includes a liquid outlet pipeline, which is connected to the liquid outlet channel 203. A third valve 404 and a second peristaltic pump 405 are sequentially installed along the liquid flow direction of the liquid outlet pipeline. When the third valve 404 is controlled to be in a connected state, the liquid in the container 301 can be pumped out by the second peristaltic pump 405.

[0072] Embodiment 2

[0073] The other technical solutions of this embodiment are the same as those of the first embodiment, except that the installation method of the container 301 is as follows: Figure 2-6 As shown, the hole 204 does not penetrate the detection plate 201. The side wall of the hole 204 is provided with a card slot 205, and the outer wall of the container 301 is provided with a card strip 303. When the container 301 needs to be installed in the hole 204, the card strip 303 can be aligned with the card slot 205, and then inserted into the container 301. In order to improve the ease of use, the positions of the card strip 303 and the card slot 205 can be set so that when the card strip 303 is inserted into the card slot 205, the outlet 305 is aligned with the liquid outlet channel 203, and the inlet 302 is aligned with the liquid inlet channel 202. It should be noted that the connection method between the container 301 and the detection plate 204 is not limited to the above-mentioned implementation scheme, and other existing technologies that can realize the detachable connection between the container 301 and the detection plate 204 are all applicable, which will not be limited or repeated here.

[0074] like Figure 8As shown, the light source 406-1 and the photoelectric detection module 406-2 of the detection module are respectively located on both sides of the liquid outlet pipeline and are arranged correspondingly. At this time, the liquid in the container 301 is pumped out into the liquid outlet pipeline by the second peristaltic pump 405. When the liquid to be detected passes through the liquid outlet pipeline, the light source 406-1 irradiates and the photoelectric detection module 406-2 detects the liquid.

[0075] Embodiment 3

[0076] The other technical solutions of this embodiment are the same as those of the first embodiment, except that the installation method of the container 301 is as follows: Figure 10-11 As shown, the hole 204 is set through the detection plate 201. There are multiple containers 301, and the multiple containers 301 are stacked and fixedly connected up and down. The fixed connection method can be bonding, or multiple containers 301 can be integrally formed, which will not be described here.

[0077] It also includes a container driving mechanism 306, which is connected to the container 301, and can drive the containers 301 to move axially along the hole position 204 one by one through the container driving mechanism 306. The side wall of the container 301 is provided with an inlet 302 and an outlet 305. When the current container is used up, the container driving mechanism 306 drives the container group to move, so that the inlet 302 and the outlet 305 of the next container are aligned with the liquid inlet channel 202 and the liquid outlet channel 203 respectively, and there is no need to remove the detection plate 201 from the detector 101 to replace the container, so there is no need to interrupt the ongoing detection process of the containers 301 at other holes in the detection plate 201.

[0078] like Fig. 9 As shown, the detection plate 201 and the container 301 are both made of light-transmitting materials. The holes 204 on the detection plate 201 are arranged in a ring shape.

[0079] The detection plate 201 is connected to the detection plate driving mechanism 601, which is used to drive the detection plate 201 to rotate step by step between eight stations. The detection plate driving mechanism 601 can use a driving mechanism with a stepper motor as a power source, which is relatively mature in the prior art and will not be described here.

[0080] The eight stations include a wastewater inlet station, a first washing station, a second antibody addition station, a second washing station, a color developer addition station, an incubation color development station, an absorbance detection station, and a container replacement station. The filtered wastewater is input into the container 301 by the microfluidic pipeline at the wastewater inlet station, and then the detection plate 201 rotates, and the container 301 with wastewater is rotated to the first washing station for washing to remove unbound antibiotics. Subsequently, the detection plate 201 rotates, and the washed container 301 rotates to the second antibody addition station, and the labeled second antibody solution is added through the microfluidic pipeline for incubation. Then the detection plate 201 rotates, and the container 301 with the second antibody added rotates to the second washing station for washing again to remove the unbound second antibody. However, the detection plate 301 rotates, and the washed container 301 rotates to the color developer addition station, and the color development solution is added to the container 301. Then the container 301 rotates to the incubation color development station, and after color development, it rotates to the absorbance detection station for detection through the photoelectric module to determine the antibiotic concentration in the livestock and poultry wastewater. After the detection is completed, the container 301 rotates to the container replacement station, and the container drive mechanism 306 drives the container group to move and replace the next new container 301. The eight stations can work at the same time, thereby realizing continuous detection.

[0081] The wastewater inlet station, the first washing station, the secondary antibody addition station, the second washing station, and the developer addition station are all provided with a pin drive mechanism 501, which is used to drive a pair of pins 502 to be inserted into or out of the liquid inlet channel 202, the inlet 302 and the liquid outlet channel 203, the outlet 305, respectively, so as to avoid liquid leakage. The pin drive mechanism 501 can use a linear push rod, which is relatively mature in the prior art and will not be described in detail here.

[0082] The pin 502 corresponding to the inlet 302 is connected to the liquid inlet pipeline through the connecting tube 503. In the first washing station and the second washing station, the pin 502 corresponding to the outlet 305 is connected to the liquid outlet pipeline through the connecting tube 503, which is convenient for extracting the washing liquid. In the wastewater inlet station, the secondary antibody addition station, and the color developer addition station, a plug 504 is installed in the pin 502 corresponding to the outlet 305 to prevent liquid leakage. Since the amount of liquid added to the container is small, when the container 301 rotates to the next station, the pin 502 can withdraw from the outlet 305 and the inlet 302, and there is no need to worry about liquid leakage.

[0083] In this solution, the light source 406-1 and the photoelectric detection module 406-2 of the detection module are arranged at the absorbance detection station and are respectively located on both sides of the hole 204 in the radial direction and are arranged correspondingly. Since the detection plate 201 and the container 301 are both made of light-transmitting materials, they can be directly detected.

[0084] The detection plate 201 is provided with a detection plate vent 206, and the container 301 is provided with a container vent 307. When the container 301 enters the hole 204, the container vent 307 is aligned with the detection plate vent 206, so that the internal air pressure of the container 301 is maintained at atmospheric pressure, and the liquid is input into the container 301 more smoothly.

[0085] Embodiment 4

[0086] The other technical solutions of this embodiment are the same as those of the above embodiment, except that a solid phase extraction module can also be provided, through which the antibiotics in the wastewater are concentrated, and then the concentrated antibiotics are input into the container 301 through the liquid inlet channel 202 through the microfluidic pipeline. The solid phase extraction module is relatively mature in the prior art and will not be described here. Due to the concentration, the volume of the container 301 can be reduced, thereby reducing the manufacturing cost of the container 301. At this time, the detection plate 201 can be used multiple times, and although the container 301 needs to be discarded after use, due to its small size, the cost of use is not high.

[0087] Embodiment 5

[0088] In this embodiment, the present application also proposes a method for using a device for detecting the antibiotic content in livestock wastewater, comprising the following steps:

[0089] The bottom layer of the inner wall of the container 301 is coated, washed, and sealed to form a capture layer 304;

[0090] Place the container 301 in the hole 204, and make the inlet 302 and outlet 305 of the container 301 communicate with the liquid inlet channel 202 and the liquid outlet channel 203 respectively; according to the use requirements, the capture layers 304 set in the containers 301 placed in each hole 204 of the detection plate 201 can be the same or different. When the capture layers 304 set in the containers 301 placed in each hole 204 are the same, each liquid inlet channel 202 can be connected to the same microfluidic pipeline. When the capture layers 304 set in the containers 301 placed in each hole 204 are different, the microfluidic pipelines connected to the liquid inlet channels 202 corresponding to different containers 301 should be different.

[0091] First, the standard sample is tested and the standard curve is fitted. Then the livestock wastewater is filtered, the filtered wastewater is input into the container 301, and washed, and then the labeled secondary antibody solution is added to the container, and then washed, and then the color developer is added to the container 301, and after color development, it is detected by the detection module to determine the antibiotic concentration in the livestock wastewater.

[0092] When the hole 204 does not penetrate the detection plate 201 , a container 301 is placed in each hole 204 . After the detection is completed, the containers 301 in all the holes 204 can be replaced uniformly.

[0093] When the hole 204 penetrates the detection plate 201, multiple stacked fixedly connected containers 301 can be inserted into the hole 204. When the current container is used up, the container drive mechanism 306 drives the container group to move so that the inlet 302 and outlet 305 of the next container are aligned with the liquid inlet channel 202 and the liquid outlet channel 203 respectively.

[0094] The present invention and its embodiments are described schematically above, and the description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. Therefore, if a person skilled in the art is inspired by it and designs a structural method and an embodiment similar to the technical solution without creativity without departing from the purpose of the invention, they shall all fall within the protection scope of the present invention.

Claims

1. A device for detecting the antibiotic content in livestock wastewater, comprising a housing, in which a detection plate (201) is installed; Features: The detection plate (201) is provided with a plurality of holes (204); The detection plate (201) is provided with a liquid inlet channel (202) and a liquid outlet channel (203) which are in communication with the hole (204); A container (301) is installed in the hole (204), and the container (301) is provided with an inlet (302) and an outlet (305) which can be communicated with the liquid inlet channel (202) and the liquid outlet channel (203) respectively; The bottom layer of the inner wall of the container (301) is provided with a capture layer (304); It also includes a microfluidic pipeline connected to the liquid inlet channel (202) and the liquid outlet channel (203), and the microfluidic pipeline is used to input liquid into the container (301) through the liquid inlet channel (202) and to extract the liquid in the container (301) through the liquid outlet channel (203); It also includes a detection module, which is used to detect the antibiotic content in the liquid in the container (301) or the liquid extracted from the container (301).

2. The device for detecting the antibiotic content in livestock wastewater according to claim 1, characterized in that: The microfluidic pipeline includes a plurality of liquid inlet pipelines, and the liquid inlet pipelines are connected to the liquid inlet channel (202); A first valve (401), a first peristaltic pump (402), and a second valve (403) are sequentially installed along the liquid inlet pipeline in the direction of liquid flow; The microfluidic pipeline also includes a liquid outlet pipeline, which is connected to the liquid outlet channel (203); A third valve (404) and a second peristaltic pump (405) are sequentially installed along the liquid flow direction of the liquid outlet pipeline.

3. The device for detecting the antibiotic content in livestock wastewater according to claim 1, characterized in that: The detection board (201) comprises a first board (201-1), a second board (201-2), and a third board (201-3) which are stacked in sequence; The adjacent surfaces of the first plate (201-1) and the second plate (201-2) form the liquid inlet channel (202); and the adjacent surfaces of the second plate (201-2) and the third plate (201-3) form the liquid outlet channel (203).

4. The device for detecting the antibiotic content in livestock wastewater according to claim 2, characterized in that: The side wall of the hole (204) is provided with a clamping groove (205); the outer wall of the container (301) is provided with a clamping strip (303); When the container (301) is placed in the hole (204) and the clamping strip (303) is inserted into the clamping groove (205), the outlet (305) is aligned with the liquid outlet channel (203), and the inlet (302) is aligned with the liquid inlet channel (202).

5. The device for detecting the antibiotic content in livestock wastewater according to claim 4, characterized in that: The light source (406-1) and the photoelectric detection module (406-2) of the detection module are respectively located on both sides of the liquid outlet pipeline and are arranged correspondingly.

6. The device for detecting the antibiotic content in livestock wastewater according to claim 2, characterized in that: The detection plate (201) and the container (301) are both made of light-transmitting materials; the holes (204) on the detection plate (201) are arranged in a ring shape; The detection plate (201) is connected to a detection plate driving mechanism (601), and the detection plate driving mechanism (601) is used to drive the detection plate (201) to rotate step by step between eight stations; The eight workstations include the wastewater inlet station, the first washing station, the secondary antibody addition station, the second washing station, the color developer addition station, the incubation color development station, the absorbance detection station, and the container replacement station; The wastewater inlet station, the first washing station, the secondary antibody addition station, the second washing station, and the developer addition station are all provided with a pin drive mechanism (501), and the pin drive mechanism (501) is used to drive a pair of pins (502) to be respectively inserted into or separated from the liquid inlet channel (202), the inlet (302) and the liquid outlet channel (203), the outlet (305); The insertion pin (502) corresponding to the inlet (302) is connected to the liquid inlet pipeline through the connecting pipe (503); In the first washing station and the second washing station, the pin (502) corresponding to the outlet (305) is connected to the liquid outlet pipeline through the connecting pipe (503); In the wastewater inlet station, the secondary antibody addition station, and the color developer addition station, a plug (504) is installed in the insertion pin (502) corresponding to the outlet (305); The light source (406-1) and the photoelectric detection module (406-2) of the detection module are arranged at the absorbance detection station and are respectively located on both sides of the hole (204) in the radial direction and are arranged correspondingly.

7. The device for detecting the antibiotic content in livestock wastewater according to claim 6, characterized in that: The hole (204) is arranged to penetrate the detection plate (201); The container (301) comprises a plurality of containers, and the plurality of containers (301) are stacked and fixedly connected up and down; It also includes a container driving mechanism (306), which is used to drive the containers (301) to move axially along the hole positions (204) one by one.

8. The device for detecting the antibiotic content in livestock wastewater according to any one of claims 1 to 6, characterized in that: It also includes a solid phase extraction module, which is used to concentrate antibiotics in wastewater, and the microfluidic pipeline is used to input the concentrated antibiotics into the container (301) through the liquid inlet channel (202).

9. A method for using the device for detecting the antibiotic content in livestock wastewater according to any one of claims 1 to 8, characterized in that: The following steps are involved: The container (301) is placed in the hole (204), and the inlet (302) and the outlet (305) of the container (301) are connected to the liquid inlet channel (202) and the liquid outlet channel (203) respectively; The livestock and poultry wastewater is filtered, and the filtered wastewater is input into a container (301) and washed. A labeled secondary antibody solution is then added to the container, and the wastewater is washed again. A color developer is then added to the container (301). After color development, the wastewater is tested by a detection module to determine the antibiotic concentration in the livestock and poultry wastewater.

10. The method for using the device for detecting the antibiotic content in livestock wastewater according to claim 9, characterized in that: Before placing the container (301) in the hole position (204), the bottom layer of the inner wall of the container (301) is coated, washed and sealed to form a capture layer (304).

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