A method for detecting and analyzing phospholipids in infant formula milk powder

Abstract

The present invention relates to the field of detection, and particularly to a method for detecting and analyzing phospholipids in infant formula milk powder. This method can predict the phospholipid content in the user's breast milk, so as to determine a matching milk powder for the user, enabling the user to feed the baby with a combination of the matching milk powder and their own breast milk, providing a reasonable amount of phospholipids for the baby to ensure the healthy growth of the baby. Moreover, since the phospholipid content in the user's breast milk changes during the lactation period, this application can predict the change trend of the phospholipid supply caused by this change during the lactation period, identify the time periods when the phospholipid supply is out of range, and give corresponding feeding method adjustment suggestions for these time periods, so that the phospholipid supply given to the baby by the user remains at a reasonable level throughout the lactation period.

Description

Technical Field

[0001] The present invention relates to the field of detection, and particularly to a method for detecting and analyzing phospholipids in infant formula milk powder. Background Art

[0002] Phospholipids play an important role in the brain development, nervous system development, liver protection and health care of infants. Breast milk contains various types of phospholipids, which can effectively ensure the growth and development of infants.

[0003] Under the condition of permission, parents prefer to breastfeed infants. However, due to insufficient milk secretion or other factors, many families can only adopt the way of combining breast milk with milk powder to feed infants. Since the content of various phospholipids in breast milk varies due to the diet structure of lactating mothers. In addition, the phospholipid content of different milk powders on the market is different, and the component content of various phospholipids is not specifically marked. Therefore, when most families choose milk powder, it is difficult to consider the complementary problem of the phospholipid content between milk powder and breast milk secreted by lactating mothers, which may lead to the problem of insufficient or excessive phospholipid intake by infants (which is likely to cause gastrointestinal discomfort in infants) when they are fed in the way of combining breast milk with milk powder, which is not conducive to the physical health of infants. Summary of the Invention

[0004] Based on this, in view of the above problems, it is necessary to provide a method for detecting and analyzing phospholipids in infant formula milk powder, and the method includes:

[0005] S1: Determine the content of various phospholipids in each milk powder by liquid chromatography-tandem mass spectrometry, and establish a milk powder phospholipid content database according to the determination results;

[0006] S2: When receiving an analysis request from any user, inquire about the user's diet structure and breastfeeding information, where the breastfeeding information includes the breastfeeding amount and the milk powder consumption;

[0007] S3: Determine the change curve of the phospholipid content in the user's breast milk during the breastfeeding period according to historical data and diet structure;

[0008] S4: Determine the phospholipid supply curve based on the breastfeeding amount and the change curve of phospholipid content;

[0009] S5: Determine the matching milk powder for the user from the milk powder phospholipid content database, and determine the increased amount of phospholipid supply corresponding to the matching milk powder to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range;

[0010] S6: Identify the out-of-range section of the phospholipid supply curve, and adjust the feeding method according to the out-of-range section, and correspondingly adjust the phospholipid supply curve so that the phospholipid supply curve is always within the preset phospholipid content range;

[0011] S7: Send the adjusted phospholipid supply curve, the corresponding adjustment time points, and the adjusted feeding method to the user.

[0012] Preferably, determining the content of various phospholipids in each kind of milk powder by liquid chromatography-tandem mass spectrometry includes:

[0013] Prepare the determination solution: Weigh 2 g of the milk powder sample into a 50 mL centrifuge tube, add 20 mL of water, and record the total mass of the milk powder sample and water; after vortex mixing evenly, place the sample solution in a constant temperature shaking water bath at 40 °C and heat for 30 min; weigh 0.5 g of the sample solution into a 15 mL centrifuge tube, add 0.9 mL of pure water and then add 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube on an ultrasonic cleaner for ultrasonic cleaning at 40 °C for 10 min, place the centrifuge tube on a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, add 5 mL of dichloromethane-methanol solution again, mix well, place the centrifuge tube on an ultrasonic cleaner for ultrasonic treatment at 40 °C for 10 min, place the centrifuge tube on a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, combine the lower layer liquids collected twice, filter through a 0.22 μm microporous filter membrane, and dissolve and make up the volume with dichloromethane-methanol to obtain the determination solution.

[0014] Perform on-machine determination of the determination solution by a liquid chromatography-tandem mass spectrometer to obtain the phospholipid concentration in the determination solution;

[0015] The liquid chromatography-tandem mass spectrometer transmits the phospholipid concentration to a computer device, and the computer device calculates the phospholipid content of the sample based on the phospholipid concentration;

[0016] Preferably, the diet structure is the food varieties and the corresponding intake amounts of the user within a set diet cycle in the past; the historical data includes the content of each phospholipid in the colostrum of lactating women under various different diet structures, and the average change ratio of each phospholipid at each preset time point in the lactation cycle; determining the change curve of the phospholipid content in the user's breast milk during the lactation cycle based on the historical data and the diet structure includes:

[0017] S31: Compare the user's diet structure with each diet structure in the historical data to determine the first content of each phospholipid in the user's colostrum;

[0018] S32: For each kind of phospholipid, generate a first coordinate system, where the abscissa of the first coordinate system is the lactation cycle and the ordinate is the content of this kind of phospholipid;

[0019] S33: Determine the second content of each phospholipid at each preset time point during the lactation period based on the first content of the phospholipid and the corresponding average change ratio, and obtain the coordinate points corresponding to each preset time point. The abscissa of the coordinate point is the preset time point, and the ordinate is the corresponding second content.

[0020] S34: Mark the coordinate points in the first coordinate system and connect the coordinate points with a smooth curve to obtain the content change curve of the phospholipid in the user's breast milk.

[0021] Preferably, comparing the user's diet structure with the diet structures in the historical data to determine the first content of each phospholipid in the user's colostrum includes:

[0022] For each phospholipid, determine the associated element of the phospholipid and the associated food types of the associated element;

[0023] Calculate the first intake of the associated element by the user according to the intake amount of the associated food varieties by the user;

[0024] Calculate the second intakes of the associated element by each lactating woman in the historical data;

[0025] Select a second intake greater than the first intake and a second intake less than the first intake, and identify the interpolation ratio of the first intake between the two second intakes;

[0026] Find the difference in the content of the phospholipid corresponding to the two second intakes, and determine the first content based on the difference and the interpolation ratio.

[0027] Preferably, the colostrum is the breast milk at the beginning of the lactation period, the preset time point corresponding to the colostrum is the starting point of the lactation period, and the average change ratio is the ratio of the content of a phospholipid in the breast milk at the preset time point to the content of the corresponding phospholipid in the colostrum;

[0028] Determining the second content of each phospholipid at each preset time point during the lactation period based on the first content of the phospholipid and the corresponding average change ratio includes:

[0029] For each phospholipid, retrieve the average change ratio corresponding to each time point of the phospholipid;

[0030] Multiply the first content by each retrieved average change ratio to obtain the second content corresponding to each time point.

[0031] Preferably, determining the phospholipid supply curve based on the lactation amount and the phospholipid content change curve includes:

[0032] For each first coordinate system, a second coordinate system is generated, where the abscissa of the second coordinate system is the feeding cycle and corresponds to the abscissa of the first coordinate system, and the ordinate is the corresponding phospholipid supply amount;

[0033] Multiply the ordinate of each coordinate point on the phospholipid content change curve in the first coordinate system by the milk feeding amount to obtain the corresponding phospholipid supply amount, and then obtain a coordinate point on the second coordinate system;

[0034] Connect the coordinate points on each second coordinate system with a smooth curve to obtain the phospholipid supply amount curve.

[0035] Preferably, determining the matching milk powder for the user from the milk powder phospholipid content database includes:

[0036] S51: Select a milk powder from the milk powder phospholipid content database as the alternative milk powder;

[0037] S52: For each phospholipid, multiply the content of this phospholipid in the alternative milk powder by the milk powder dosage to obtain the increased supply amount of this phospholipid;

[0038] S53: In the second coordinate system corresponding to this phospholipid, simulate and raise the phospholipid supply amount curve by the increased supply amount;

[0039] S54: Generate a first horizontal line and a second horizontal line on this second coordinate system, where the first horizontal line corresponds to the preset minimum content of this phospholipid, and the first horizontal line corresponds to the preset maximum content of this phospholipid;

[0040] S55: Identify the curve point on the phospholipid supply amount curve that is first lower than the first horizontal line or first higher than the second horizontal line, and then identify the abscissa of this curve point as the over-limit time point;

[0041] S56: Determine the earliest time point among the over-limit time points corresponding to each phospholipid as the target time point of this alternative milk powder;

[0042] S57: Repeat steps S51 to S56 until the target time point of each alternative milk powder is obtained, and determine the alternative milk powder with the latest corresponding target time point as the matching milk powder;

[0043] Adjust the phospholipid supply amount curve, that is, raise the entire phospholipid supply amount curve by the increased supply amount of the phospholipid corresponding to the matching milk powder.

[0044] Preferably, identifying the out-of-range section of the phospholipid supply amount curve and adjusting the feeding method according to the out-of-range section, and correspondingly adjusting the phospholipid supply amount curve includes:

[0045] S61: Determine the earliest time point among the over-limit time points of each phospholipid supply amount curve as the adjustment time point;

[0046] S62: Alter the dosage ratio of the milk intake and the milk powder dosage after the adjustment time point, so that the respective phospholipid supply curves are correspondingly adjusted in the section after the adjustment time point;

[0047] S63: Determine whether the adjustment time point can be postponed by adjusting the dosage ratio. If so, determine the dosage ratio with the longest postponed duration of the adjustment time point as the target ratio, adjust the section of the phospholipid supply curve after the adjustment time point according to the target ratio, then determine the new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the nursing cycle;

[0048] S64: If not, re-determine the matching milk powder for the section after the adjustment time point, and correspondingly adjust the section after the adjustment time point, so as to obtain a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the nursing cycle.

[0049] Preferably, determining whether the adjustment time point can be postponed by adjusting the dosage ratio includes:

[0050] S631: Determine all dosage ratios;

[0051] S632: Select a dosage ratio. For each phospholipid supply curve, adjust the section of the phospholipid supply curve after the adjustment time point according to the dosage ratio, and identify the over-limit time point of this section after adjustment;

[0052] S633: Determine the earliest time point among the over-limit time points corresponding to each phospholipid supply curve, and identify whether the earliest time point is later than the adjustment time point. If so, it is determined that the adjustment time point can be postponed by adjusting the dosage ratio;

[0053] S634: If not, execute steps S632 to S634 until it is determined that the adjustment time point can be postponed by adjusting the dosage ratio, or the earliest time point can never be later than the adjustment time point, then it is determined that the adjustment time point cannot be postponed by adjusting the dosage ratio.

[0054] The present invention provides a method for detecting and analyzing phospholipids in infant formula milk powder. The content of various phospholipids in each milk powder is determined by liquid chromatography-tandem mass spectrometry, and a milk powder phospholipid content database is established according to the determination results. When receiving an analysis request from any user, the user's diet structure and breastfeeding information are queried. According to historical data and diet structure, the change curve of phospholipid content in the user's breast milk during the breastfeeding period is determined. The phospholipid supply curve is determined based on the breastfeeding volume and the change curve of phospholipid content. The matching milk powder for the user is determined from the milk powder phospholipid content database, and the increased amount of phospholipid supply corresponding to the matching milk powder is determined to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range. The out-of-range section of the phospholipid supply curve is identified, and the feeding method is adjusted based on the out-of-range section. Accordingly, the phospholipid supply curve is adjusted so that the phospholipid supply curve is always within the preset phospholipid content range. The adjusted phospholipid supply curve, the corresponding adjustment time point, and the adjusted feeding method are sent to the user. In this application, it is possible to predict the phospholipid content in the user's breast milk, thereby determining a matching milk powder for the user, enabling the user to feed the baby with a combination of the matching milk powder and their own breast milk, providing a reasonable amount of phospholipids for the baby, and ensuring the healthy growth of the baby. Moreover, since the phospholipid content in the user's breast milk changes during the breastfeeding period, this application can predict the change trend of phospholipid supply caused by this change during the breastfeeding period, identify the out-of-range period of phospholipid supply, and give corresponding feeding method adjustment suggestions for this period, so that the phospholipid supply given to the baby by the user remains at a reasonable level throughout the breastfeeding period. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1 It is a flowchart of a method for detecting and analyzing phospholipids in infant formula milk powder provided in an embodiment.

[0056] Figure 2 It is an implementation environment diagram of a method for detecting and analyzing phospholipids in infant formula milk powder provided in an embodiment.

[0057] Figure 3 It is a schematic diagram of the adjustment of the phospholipid supply curve of a method for detecting and analyzing phospholipids in infant formula milk powder provided in an embodiment. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0058] In order to make the objectives, technical solutions, and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0059] It can be understood that the terms "first", "second", etc. used in the present invention may be used herein to describe various elements, but unless otherwise specified, these elements are not limited by these terms. These terms are only used to distinguish the first element from another element. For example, without departing from the scope of the present invention, the first xx script may be referred to as the second xx script, and similarly, the second xx script may be referred to as the first xx script.

[0060] As Figure 1 shown, in one embodiment, a method for detecting and analyzing phospholipids in infant formula milk powder is proposed, and the method includes:

[0061] S1: Determine the content of various phospholipids in each kind of milk powder by liquid chromatography-tandem mass spectrometry, and establish a milk powder phospholipid content database according to the determination results;

[0062] S2: When receiving an analysis request from any user, inquire about the user's diet structure and breastfeeding information, where the breastfeeding information includes the breastfeeding amount and the milk powder consumption;

[0063] S3: Determine the change curve of the phospholipid content in the user's breast milk during the breastfeeding period according to the historical data and the diet structure;

[0064] S4: Determine the phospholipid supply curve based on the breastfeeding amount and the change curve of the phospholipid content;

[0065] S5: Determine the matching milk powder for the user from the milk powder phospholipid content database, determine the increased amount of phospholipid supply corresponding to the matching milk powder, and adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range;

[0066] S6: Identify the out-of-range section of the phospholipid supply curve, and adjust the feeding method according to the out-of-range section, and correspondingly adjust the phospholipid supply curve so that the phospholipid supply curve is always within the preset phospholipid content range;

[0067] S7: Send the adjusted phospholipid supply curve, the corresponding adjustment time point, and the adjusted feeding method to the user.

[0068] In this embodiment, as Figure 2As shown, the step of "determining the content of various phospholipids in each kind of milk powder by liquid chromatography-tandem mass spectrometry" in step S1 is executed by the staff in cooperation with a liquid chromatography-tandem mass spectrometer and a computer device, and the step of "establishing a milk powder phospholipid content database according to the determination results" in step S1 is executed by the computer device; steps S2 to S7 are executed by the computer device; the computer device can be an independent physical server or terminal, or a server cluster composed of multiple physical servers, and can be a cloud server providing basic cloud computing services such as cloud servers, cloud databases, cloud storage, and CDN; the user communicates with the computer device through a user terminal (such as a mobile phone, laptop, tablet computer, etc.), so that the computer device can receive the user's analysis request and interact with the user terminal for information.

[0069] In this embodiment, each kind of milk powder can be all the milk powders sold on the market; milk powder often contains various phospholipids, such as phosphatidylcholine, phosphatidylinositol, sphingomyelin, etc.; the analysis request is a request sent by the user for the computer device to determine the required milk powder and how to match the breastfeeding amount and milk powder dosage; when the computer device receives the analysis request, it first sends authorization information to the user terminal, and the authorization information includes the execution steps of this method and the user data to be called, for the user to understand its process. After the user sends back the consent information through the user terminal, this method can be executed.

[0070] In this embodiment, the diet structure is the food varieties and corresponding intake amounts of the user within a set past diet cycle (such as a month or other duration); different diet structures will provide different elemental components for the synthesis of breast milk, thereby resulting in different contents of various phospholipids in breast milk; in this embodiment, the historical data is data that can reflect the relationship between the diet structure and the phospholipid content in breast milk. Therefore, the content of various phospholipids in the user's breast milk can be determined through the historical data and the diet structure. Further, based on this content and the breastfeeding amount (the breastfeeding amount is the amount of breast milk used for breastfeeding per day, and the user can use a milk bottle to collect their breast milk to determine the milk amount), the amount of various phospholipids that the user can provide to the baby through breastfeeding can be determined, thereby obtaining a phospholipid supply curve.

[0071] In this embodiment, the established milk powder phospholipid content database includes the content of each type of milk powder and each phospholipid corresponding to the milk powder; based on the data in the database and the user's milk powder consumption, it is possible to determine the increment that each type of milk powder can bring to the phospholipid supply curve. After adjusting the phospholipid supply curve by the increment brought by each type of milk powder, the matching milk powder can be determined, that is, the initial section of the phospholipid supply curve can be made to fall within the preset phospholipid content range (a phospholipid content range is preset for each phospholipid, and the initial section of the phospholipid supply curve corresponding to each phospholipid needs to fall within the corresponding phospholipid content range); the initial section is the section corresponding to the set duration (such as one month) from the start of the feeding cycle on the phospholipid supply curve; if multiple milk powders can make the initial section fall within the corresponding phospholipid content range, the milk powder corresponding to the latest time point when the phospholipid supply curve first exceeds the range is selected as the matching milk powder; through the feeding method corresponding to the phospholipid supply curve (that is, the combined feeding of breast milk and the matching milk powder according to the breastfeeding amount and milk powder content given by the user), it is possible to ensure that the supply of various phospholipids to the baby is within the corresponding phospholipid content range during the time period corresponding to the initial section; further, the phospholipid supply curve often cannot be within the preset content range throughout the breastfeeding cycle (the duration from childbirth to the end of breastfeeding, which is taken as the historical statistical average in this embodiment, such as 2 years). At this time, this embodiment can also identify the out-of-range section in the phospholipid supply curve and update the feeding method (such as updating the breastfeeding amount and milk powder consumption or changing the milk powder) at the adjustment time point corresponding to the out-of-range section (that is, the time point corresponding to the starting point of the out-of-range section) to make the out-of-range section return to the preset content range, so as to keep the phospholipid supply to the baby within a reasonable range all the time; after the adjustment is completed, the corresponding new feeding method is marked at the position of the phospholipid supply curve corresponding to the adjustment time point, and then the phospholipid supply curve is sent to the user terminal, and the user can intuitively know when and what kind of feeding method should be adopted.

[0072] In this application, it is possible to predict the phospholipid content in the user's breast milk, thereby determining a matching milk powder for the user, enabling the user to feed the baby with a combination of the matching milk powder and their own breast milk, providing a reasonable amount of phospholipids for the baby, ensuring the healthy growth of the baby. And because the phospholipid content in the user's breast milk will change during the breastfeeding cycle, this application can predict the change trend of the phospholipid supply caused by this change during the breastfeeding cycle, identify the out-of-range period of the phospholipid supply, and give corresponding feeding method adjustment suggestions for this period, so that the phospholipid supply given by the user to the baby remains at a reasonable level throughout the breastfeeding cycle.

[0073] As a preferred embodiment, determining the content of various phospholipids in each type of milk powder by liquid chromatography-tandem mass spectrometry includes:

[0074] Preparation of the assay solution: Weigh 2 g of the milk powder sample into a 50 mL centrifuge tube, add 20 mL of water, and record the total mass of the milk powder sample and water. After vortex mixing evenly, place the sample solution in a constant temperature shaking water bath at 40 °C and heat for 30 min. Weigh 0.5 g of the sample solution into a 15 mL centrifuge tube, add 0.9 mL of pure water, then add 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube on an ultrasonic cleaner and ultrasonically clean at 40 °C for 10 min, place the centrifuge tube on a centrifuge and centrifuge at 8000 r / min for 10 min, collect the lower layer liquid, add another 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube on an ultrasonic cleaner and ultrasonically clean at 40 °C for 10 min, place the centrifuge tube on a centrifuge and centrifuge at 8000 r / min for 10 min, collect the lower layer liquid, combine the lower layer liquids collected twice, filter through a 0.22 μm microporous filter membrane, and dissolve and make up the volume with dichloromethane-methanol to obtain the assay solution.

[0075] The assay solution is measured on a liquid chromatography-tandem mass spectrometry instrument to obtain the phospholipid concentration in the assay solution.

[0076] The liquid chromatography-tandem mass spectrometry instrument transmits the phospholipid concentration to a computer device, and the computer device calculates the phospholipid content of the sample based on the phospholipid concentration.

[0077] In this embodiment, the computer device calculates the content of any phospholipid in the milk powder according to the following formula:

[0078]

[0079] Among them, X is the content of this phospholipid, is the concentration of this phospholipid in the assay solution, m is the sampling mass, and V is the volume of the assay solution for volume determination.

[0080] In this embodiment, the unit of C is g / mL, the unit of V is mL, and the unit of m is g; for detecting different phospholipids, the corresponding isotope internal standard working solutions are also different. For example, to detect phosphatidylcholine, the isotope internal standard working solution of phosphatidylcholine is prepared in advance, and the preparation steps are as follows: Accurately weigh 10 mg of phosphatidylcholine standard (accurate to 0.00001 g), place it in a 10 mL brown volumetric flask, dissolve and make up the volume to the scale with 0.1% formic acid methanol solution to prepare a standard stock solution with a concentration of 1000 μg / mL, and store it below -18 °C.

[0081] In this embodiment, before the measurement solution is measured on the instrument, it is necessary to prepare a standard curve series solution of the phospholipids to be measured, which includes solutions of phospholipids with multiple standard concentrations from small to large (such as 0.5 μg / mL, 1 μg / mL, 1.5 μg / mL, etc.). The solutions are measured on the instrument in ascending order of concentration. A standard curve is plotted with the phospholipid concentration as the abscissa and the peak area of the phospholipid isotope internal standard as the ordinate. Then, the measurement solution is measured to obtain the curve of the measurement solution. By comparing the positions of the measurement solution curve and the standard curve, the concentration of the analyte in the measurement solution can be quantified.

[0082] The chromatographic conditions for detection include:

[0083] Temperature: 50 °C;

[0084] Sample injection volume: 5 μL;

[0085] Mobile phase A: isopropanol - acetonitrile (1:1); Mobile phase B: 20 mmol / L ammonium formate aqueous solution;

[0086] Flow rate: 0.3 mL / min;

[0087] The mass spectrometry conditions include:

[0088] Ion source: electrospray ionization source, temperature 250 °C;

[0089] Scanning mode: positive ion mode and negative ion mode;

[0090] Collision gas: argon;

[0091] Heating block temperature: 400 °C

[0092] Desolvation temperature: 650 °C;

[0093] Desolvation gas flow rate: 900 L / h;

[0094] Capillary voltage: 1.0 kV.

[0095] As a preferred embodiment, the dietary structure is the types of food and the corresponding intake of the user within a set dietary cycle in the past; the historical data includes the content of each phospholipid in the colostrum of lactating women under various different dietary structures, and the average change ratio of various phospholipids at each preset time point during the lactation cycle; determining the change curve of the phospholipid content in the breast milk of the user during the lactation cycle based on the historical data and the dietary structure includes:

[0096] S31: Compare the dietary structure of the user with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum;

[0097] S32: For each type of phospholipid, generate a first coordinate system, where the abscissa of the first coordinate system is the lactation period and the ordinate is the content of this type of phospholipid;

[0098] S33: Determine the second content of this type of phospholipid at each preset time point in the lactation period based on the first content of this type of phospholipid and the corresponding average change ratio, and obtain the coordinate points corresponding to each preset time point, where the abscissa of the coordinate point is the preset time point and the ordinate is the corresponding second content;

[0099] S34: Mark the coordinate points in the first coordinate system and connect the coordinate points with a smooth curve to obtain the content change curve of this type of phospholipid in the user's breast milk.

[0100] In this embodiment, the starting point of the abscissa of the first coordinate system is the starting point of the nursing period; the historical data are the actually detected data in the past; for example, the phospholipid content in the colostrum of lactating women under each dietary structure is detected and stored for subsequent call; for the average change ratio at each preset time point in the lactation period, first detect the change ratio of each preset time point of each lactating woman, and for each preset time point, average the change ratios of the lactating women corresponding to this preset time point to obtain the average change ratio; the steps of detecting the change ratio of each preset time point (such as 1 month, 2 months, 3 months, etc. after childbirth) of each lactating woman are as follows: first detect the various phospholipid contents in the colostrum of the lactating woman, and detect the various phospholipid contents in the breast milk of this woman every time a preset time point is reached. Divide the detected content of each type of phospholipid by the content of the corresponding phospholipid in the colostrum to obtain the change ratio of this type of phospholipid at this preset time point.

[0101] As a preferred embodiment, comparing the user's dietary structure with the dietary structures in the historical data to determine the first content of each phospholipid in the user's colostrum includes:

[0102] For each type of phospholipid, determine the associated elements of this phospholipid and the associated food types of the associated elements;

[0103] Calculate the first intake of the associated elements by the user based on the intake of the associated food varieties by the user;

[0104] Calculate the second intakes of the associated elements by each lactating woman in the historical data;

[0105] Select a second intake greater than the first intake and a second intake less than the first intake, and identify the interpolation ratio of the first intake between the two second intakes;

[0106] Find the difference between the contents of this type of phospholipid corresponding to the two second intakes, and determine the first content based on this difference and the interpolation ratio.

[0107] In this embodiment, the reason why a certain phospholipid exists in breast milk is that the human body absorbs the associated elements for synthesizing this phospholipid. To obtain these associated elements, the human body needs to consume associated foods containing these elements. In this implementation, a phospholipid-associated element-associated food type comparison table is preset, which includes the associated elements of each phospholipid and the food varieties that can provide these elements (including the unit intake of associated elements corresponding to the unit intake of these food varieties). Thus, the first intake can be obtained by multiplying the unit intake by the intake amount of the corresponding associated food type by the user. In the same way, the second intakes of associated elements for lactating women in historical data can be obtained. For the interpolation ratio, for example, if the first intake is 2 g, one of the selected second intakes is 1 g, and the other second intake is 3 g. The difference between the two second intakes is 3 - 1 = 2 g, and the difference between the first intake and the smaller second intake is 2 - 1 = 1 g. Thus, the interpolation ratio is obtained as 1 / 2. Further, the phospholipid content corresponding to the smaller second intake is 0.3%, and the phospholipid content corresponding to the other second intake is 0.9%. The content difference is 0.9% - 0.3% = 0.6%. Multiplying 0.6% by the interpolation ratio gives an interpolation value of 0.3%. Then, adding the interpolation value to the smaller second intake gives 0.6%, which is the first content.

[0108] As a preferred embodiment, colostrum is the breast milk at the beginning of the lactation period. The preset time point corresponding to colostrum is the starting point of the lactation period. The average change ratio is the ratio of the content of a certain phospholipid in breast milk at the preset time point to the corresponding phospholipid content in colostrum.

[0109] Determining the second content of this phospholipid at each preset time point in the lactation period based on the first content of this phospholipid and the corresponding average change ratio includes:

[0110] For each phospholipid, retrieve the average change ratio corresponding to each time point for this phospholipid;

[0111] Multiply the first content by each retrieved average change ratio to obtain the second content corresponding to each time point.

[0112] In this embodiment, the average change ratio at the preset time point corresponding to colostrum is 1. Through this embodiment, the phospholipid content at each stage of the nursing period can be calculated, thereby obtaining the change curve of the phospholipid content to intuitively reflect the change trend of the phospholipid content during the nursing period.

[0113] As a preferred embodiment, determining the phospholipid supply curve based on the nursing amount and the phospholipid content change curve includes:

[0114] For each first coordinate system, a second coordinate system is generated, where the abscissa of the second coordinate system is the nursing period and corresponds to the abscissa of the first coordinate system, and the ordinate is the corresponding phospholipid supply amount;

[0115] Multiply the ordinate of each coordinate point on the phospholipid content change curve in the first coordinate system by the nursing amount to obtain the corresponding phospholipid supply amount, and then obtain a coordinate point on the second coordinate system;

[0116] Connect the coordinate points on each second coordinate system with a smooth curve to obtain the phospholipid supply amount curve.

[0117] In this embodiment, multiplying the phospholipid content in breast milk by the nursing amount can obtain the amount of phospholipids that can be provided to the baby by breastfeeding. Multiply the phospholipid content corresponding to each point on the phospholipid content curve by the nursing amount to obtain several supply amounts, and then the corresponding phospholipid supply amount curve can be obtained.

[0118] As a preferred embodiment, determining the matching milk powder for the user from the milk powder phospholipid content database includes:

[0119] S51: Select a milk powder from the milk powder phospholipid content database as the alternative milk powder;

[0120] S52: For each phospholipid, multiply the content of this phospholipid in the alternative milk powder by the milk powder dosage to obtain the increased supply amount of this phospholipid;

[0121] S53: In the second coordinate system corresponding to this phospholipid, simulate and increase the supply amount of the phospholipid supply amount curve by the increased supply amount;

[0122] S54: Generate a first horizontal line and a second horizontal line on this second coordinate system, where the first horizontal line corresponds to the preset minimum content of this phospholipid, and the first horizontal line corresponds to the preset maximum content of this phospholipid;

[0123] S55: Identify the curve point on the phospholipid supply amount curve that is first lower than the first horizontal line or first higher than the second horizontal line, and then identify the abscissa of this curve point as the over-limit time point;

[0124] S56: Determine the earliest time point from the over-limit time points corresponding to each phospholipid as the target time point of this alternative milk powder;

[0125] S57: Repeat steps S51 to S56 until the target time points of each alternative milk powder are obtained, and determine the alternative milk powder with the latest corresponding target time point as the matching milk powder;

[0126] Adjust the phospholipid supply amount curve, that is, lift the entire phospholipid supply amount curve by the increased supply amount of the phospholipid corresponding to the matching milk powder.

[0127] In this embodiment, for each type of milk powder, before the corresponding target time point, the phospholipid supply curve of each phospholipid is within a reasonable range (i.e., between the first horizontal line and the second horizontal line). The later the target time point is, the longer the duration that the currently determined feeding method can last, and the frequency of subsequent adjustment of the feeding method can be minimized as much as possible. Simulate the increase until the corresponding target time point is determined, and then reset the phospholipid supply curve, and then perform the process of determining the target time point of another milk powder; after finally determining the matching milk powder, immediately raise the entire phospholipid supply curve by the supply increase amount corresponding to the phospholipid of the matching milk powder, and this increase is not reset.

[0128] As Figure 3 shown, as a preferred embodiment, identify the out-of-range section of the phospholipid supply curve, and adjust the feeding method according to the out-of-range section, and the corresponding adjustment of the phospholipid supply curve includes:

[0129] S61: Determine the earliest time point among the out-of-limit time points of each phospholipid supply curve as the adjustment time point;

[0130] S62: Change the dosage ratio of the breastfeeding amount and the milk powder amount after the adjustment time point, so that the sections of each phospholipid supply curve after the adjustment time point are adjusted accordingly;

[0131] S63: Judge whether the adjustment time point can be postponed by adjusting the dosage ratio. If so, determine the dosage ratio with the longest postponed duration of the adjustment time point as the target ratio, adjust the section of the phospholipid supply curve after the adjustment time point according to the target ratio, and then determine the new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the feeding cycle;

[0132] S64: If not, re-determine the matching milk powder for the section after the adjustment time point, and adjust the section after the adjustment time point accordingly, so as to obtain a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the feeding cycle.

[0133] Judging whether the adjustment time point can be postponed by adjusting the dosage ratio includes:

[0134] S631: Determine all dosage ratios;

[0135] S632: Select a dosage ratio. For each phospholipid supply curve, adjust the section of the phospholipid supply curve after the adjustment time point according to the dosage ratio, and identify the out-of-limit time point of this section after adjustment;

[0136] S633: Determine the earliest time point among the over-limit time points corresponding to each phospholipid supply curve, and identify whether the earliest time point is later than the adjustment time point. If so, it is determined that the adjustment time point can be postponed by adjusting the dosage ratio.

[0137] S634: If not, execute steps S632 to S634 until it is determined that the adjustment time point can be postponed by adjusting the dosage ratio, or if the earliest time point can never be made later than the adjustment time point, it is determined that the adjustment time point cannot be postponed by adjusting the dosage ratio.

[0138] In this embodiment, since the feeding amount of the baby is fixed, when the breastfeeding amount increases, the milk powder dosage decreases accordingly. Steps to determine all dosage ratios: First, determine the equivalent breastfeeding amount of the unit milk powder dosage; determine the breastfeeding amount when only breast milk is used (at this time, the milk powder dosage is 0); then increase the milk powder dosage by the unit milk powder dosage, decrease the breastfeeding amount by the equivalent breastfeeding amount, and then calculate the ratio of the breastfeeding amount to the milk powder dosage to obtain a dosage ratio. Repeat this step until the breastfeeding amount is reduced to 0, and all dosage ratios can be obtained. In this timely and appropriate manner, after determining the target ratio, recalculate the phospholipid supply amount corresponding to breastfeeding and the supply increase amount corresponding to milk powder for each subsequent preset time node using the breastfeeding amount and milk powder dosage corresponding to the target ratio (the specific steps are the same as those described above and will not be elaborated here), so as to obtain an updated phospholipid supply curve (only updated after the adjustment time point).

[0139] In this embodiment, each adjustment of the phospholipid supply curve is performed on the section after the adjustment time point, that is, to inform the user that the feeding method will be adjusted after this adjustment time point, and indicate the dosage ratio at this adjustment time point to inform the user how to adjust specifically; when it is necessary to re-match the milk powder, also mark the newly matched milk powder type at the corresponding adjustment time point to inform the user that the milk powder needs to be replaced at this time point to meet the baby's phospholipid requirements.

[0140] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0141] The above-described embodiments only represent several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent should be subject to the appended claims.

Claims

1. A method for detecting and analyzing phospholipids in infant formula milk powder, characterized in that: The method comprises: S1: Determine the content of various phospholipids in each type of milk powder by liquid chromatography-tandem mass spectrometry, and establish a milk powder phospholipid content database based on the determination results; S2: When receiving an analysis request from any user, inquire about the user's diet structure and breastfeeding information, wherein the breastfeeding information includes the amount of breastfeeding and the amount of milk powder used; S3: Determine the phospholipid content change curve in the user's breast milk during the lactation cycle based on historical data and dietary structure; S4: Determine the phospholipid supply curve based on the lactation amount and the phospholipid content change curve; S5: determining the matching milk powder of the user from the milk powder phospholipid content database, and determining the phospholipid supply increase amount corresponding to the matching milk powder, so as to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range; S6: identifying the out-of-range section of the phospholipid supply curve, adjusting the feeding method according to the out-of-range section, and adjusting the phospholipid supply curve accordingly, so that the phospholipid supply curve is always within the preset phospholipid content range; S7: Send the adjusted phospholipid supply curve, the corresponding adjustment time point and the adjusted feeding method to the user.

2. The method according to claim 1, characterized in that The content of various phospholipids in each milk powder was determined by liquid chromatography-tandem mass spectrometry including: Preparation of the assay solution: weigh 2 g of the milk powder sample in a 50 mL centrifuge tube, add 20 mL of water, and record the total mass of the milk powder sample and water; after vortex mixing, heat the sample solution in a 40°C constant temperature shaking water bath for 30 min; weigh 0.5 g of the sample solution in a 15 mL centrifuge tube, add 0.9 mL of pure water and then add 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube in an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube in a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, add 5 mL of dichloromethane-methanol solution again, mix well, place the centrifuge tube in an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube in a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, combine the lower layer liquids collected twice, pass through a 0.22 μm microporous filter membrane, and dissolve and fix the volume with dichloromethane-methanol to obtain the assay solution; The test solution is measured by liquid chromatography-tandem mass spectrometry to obtain the phospholipid concentration in the test solution; The liquid chromatography-tandem mass spectrometry instrument transmits the phospholipid concentration to the computer device, and the computer device calculates the phospholipid content of the sample based on the phospholipid concentration.

3. The method according to claim 1, characterized in that The dietary structure refers to the food types and corresponding food intake of the user in a set dietary cycle in the past; the historical data includes the content of various phospholipids in the colostrum of lactating women under various dietary structures, and the average change ratio of various phospholipids at each preset time point in the lactation cycle; the phospholipid content change curve of the user's breast milk during the lactation cycle determined based on the historical data and dietary structure includes: S31: comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum; S32: For each phospholipid, a first coordinate system is generated, wherein the abscissa of the first coordinate system is the lactation cycle, and the ordinate is the content of the phospholipid; S33: determining the second content of the phospholipid at each preset time point in the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio, and obtaining a coordinate point corresponding to each preset time point, wherein the abscissa of the coordinate point is the preset time point, and the ordinate is the corresponding second content; S34: marking the coordinate points in the first coordinate system, and connecting the coordinate points with a smooth curve to obtain a content variation curve of the phospholipid in the user's breast milk.

4. The method according to claim 3, characterized in that Comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum includes: For each phospholipid, determine the associated elements of the phospholipid and the associated food types of the associated elements; Calculating a first intake of the associated element by the user according to the amount of the associated food variety consumed by the user; Calculate the second intake of related elements for each lactating woman in historical data; Selecting a second intake amount greater than the first intake amount and a second intake amount less than the first intake amount, and identifying the gap ratio of the first intake amount between the two second intake amounts; The difference between the contents of the phospholipid corresponding to the two second intakes is calculated, and the first content is determined according to the difference and the intervening ratio.

5. The method according to claim 3, characterized in that: Colostrum is breast milk at the beginning of the lactation cycle, the preset time point corresponding to colostrum is the starting point of the lactation cycle, and the average change ratio is the ratio of the content of a phospholipid in breast milk at the preset time point to the corresponding phospholipid content in colostrum; Determining the second content of the phospholipid at each preset time point during the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio includes: For each phospholipid, the average change ratio of the phospholipid corresponding to each time point is retrieved; The first content is multiplied by the average change ratio of each adjustment to obtain the second content corresponding to each time point.

6. The method according to claim 3, characterized in that The phospholipid supply curve is determined based on the lactation amount and phospholipid content change curve, including: Corresponding to each first coordinate system, a second coordinate system is generated, wherein the abscissa of the second coordinate system is the nursing period and corresponds to the abscissa of the first coordinate system, and the ordinate is the corresponding phospholipid supply; Multiply the ordinate of each coordinate point of the phospholipid content change curve in the first coordinate system by the lactation amount to obtain the corresponding phospholipid supply, and then obtain a coordinate point in the second coordinate system; The coordinate points on each second coordinate system are connected with a smooth curve to obtain a phospholipid supply curve.

7. The method according to claim 6, characterized in that The matching milk powder for the user determined from the milk powder phospholipid content database includes: S51: selecting a milk powder from a milk powder phospholipid content database as an alternative milk powder; S52: for each phospholipid, multiply the content of the phospholipid in the alternative milk powder by the amount of milk powder to obtain the increased supply of the phospholipid; S53: in the second coordinate system corresponding to the phospholipid, simulating the phospholipid supply curve to increase the supply increase; S54: generating a first horizontal line and a second horizontal line on the second coordinate system, wherein the first horizontal line corresponds to a preset minimum content of the phospholipid, and the second horizontal line corresponds to a preset maximum content of the phospholipid; S55: identifying a curve point on the phospholipid supply curve that is lower than the first horizontal line for the first time or higher than the second horizontal line for the first time, and then identifying the abscissa of the curve point as the limit-exceeding time point; S56: determining the earliest time point from the over-limit time points corresponding to each phospholipid as the target time point of the candidate milk powder; S57: Repeat steps S51 to S56 until the target time point of each candidate milk powder is obtained, and determine the candidate milk powder with the latest corresponding target time point as the matching milk powder; The phospholipid supply curve is adjusted, that is, each phospholipid supply curve is improved as a whole to match the increased supply of phospholipids corresponding to the milk powder.

8. The method according to claim 7, characterized in that Identify the out-of-range section of the phospholipid supply curve, adjust the feeding method according to the out-of-range section, and adjust the phospholipid supply curve accordingly, including: S61: determining the earliest time point among the over-limit time points of each phospholipid supply curve as the adjustment time point; S62: changing the dosage ratio of the breastfeeding amount and the milk powder dosage after the adjustment time point, so that the sections of the phospholipid supply curves after the adjustment time point are adjusted accordingly; S63: Determine whether the adjustment time point can be postponed by adjusting the dosage ratio. If so, determine the dosage ratio that postpones the adjustment time point for the longest time as the target ratio, adjust the section of the phospholipid supply curve after the adjustment time point according to the target ratio, and then determine a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the nursing cycle; S64: If not, re-determine the matching milk powder for the segment after the adjustment time point, and adjust the segment after the adjustment time point accordingly to obtain a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the feeding cycle.

9. The method according to claim 7, characterized in that: Determining whether the adjustment time point can be postponed by adjusting the dosage ratio includes: S631: Determine all dosage ratios; S632: selecting a dosage ratio, for each phospholipid supply curve, adjusting the section of the phospholipid supply curve after the adjustment time point according to the dosage ratio, and identifying the over-limit time point of the section after adjustment; S633: determining the earliest time point of the over-limit time points corresponding to each phospholipid supply curve, identifying whether the earliest time point is later than the adjustment time point, and if so, determining whether the adjustment time point can be postponed by adjusting the dosage ratio; S634: If not, execute steps S632 to S634 until it is determined that the adjustment time point can be postponed by adjusting the dosage ratio, or if the earliest time point cannot be made later than the adjustment time point, it is determined that the adjustment time point cannot be postponed by adjusting the dosage ratio.

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