Egg white avian leukosis detection operation method

Abstract

The invention relates to an egg white avian leukosis detection operation method which comprises the following steps: (1) making an egg label which at least comprises a two-dimensional code for recording information of a corresponding to-be-detected chicken; (2) collecting eggs laid by the to-be-detected chickens, and pasting corresponding egg labels on the eggs; (3) scanning the two-dimensional code on the egg label, generating a chicken information table, and making a test tube label corresponding to each chicken to be detected based on the chicken information table; (4) putting egg white of each egg into test tubes, pasting corresponding test tube labels on the test tubes, and sequentially putting the test tubes into a cryopreservation box for cryopreservation; (5) unfreezing the egg white in each test tube, and sampling the egg white in the cell culture plate in sequence; and (6) carrying out avian leukosis detection on the egg white, and filling or pasting a detection result into the chicken information table. According to the invention, the information acquisition technology is combined with the traditional avian leukosis detection technology, the detection process is optimized, and the detection efficiency and the accuracy of the detection result are improved.

Description

Technical Field

[0001] The invention relates to an egg white avian leukosis detection operation method and belongs to the technical field of poultry disease prevention and control. Background Art

[0002] Animal disease prevention and control is related to national food safety and public health safety. Avian leukosis refers to a variety of tumor diseases in chickens caused by retroviruses. It can be transmitted both horizontally and vertically, and can cause huge economic losses to chickens after infection. The National Medium- and Long-Term Animal Disease Prevention and Control Plan (2012-2020) lists avian leukosis as a domestic animal disease for priority prevention and control. The purification procedures recommended in the Technical Specification for Avian Leukosis Purification T / CVMA 1-2018 are meconium testing for hatched chicks, anal swab testing in the late brooding period, and egg white testing of 2-3 breeding eggs in the early stage of laying and before seed storage.

[0003] According to the 1:240,000 magnification effect of chickens, the health of one hen in the core group is related to 240,000 commercial chickens. Egg white testing in the early stage of production and before seed retention can timely eliminate avian leukosis-positive chickens and avoid the occurrence of avian leukosis in commercial chickens. Currently, when testing egg white in the early stage of production and before seed retention, the chicken information is mostly written on the surface of the eggs and the surface of the centrifuge tube. After the test, the information in the centrifuge tube is entered into the computer and then matched with the results. This method is prone to writing or transcription errors, resulting in incorrect chicken information and delayed feedback of test results. In addition, the process of writing, transcribing and entering information into the computer is time-consuming and inefficient. Summary of the invention

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides an egg white avian leukosis detection operation method, optimizes the detection process, and improves the detection efficiency and the accuracy of the detection results.

[0005] The technical solution of the present invention to achieve the above object is: an egg white avian leukosis detection operation method, comprising the following steps:

[0006] (1) preparing an egg label for each chicken to be inspected, wherein the egg label at least includes a QR code recording information of the corresponding chicken;

[0007] (2) collecting eggs laid by each chicken to be inspected, and attaching the corresponding egg label to each egg;

[0008] (3) Based on the QR code information conversion system, a barcode scanner is used to scan the QR code on the eggs laid by each chicken to be inspected to generate a chicken information table, and a test tube label corresponding to each chicken to be inspected is printed based on the chicken information in the chicken information table, wherein the test tube label at least includes serial number information corresponding to the arrangement order of the corresponding chicken information in the chicken information table;

[0009] (4) taking the egg whites of the eggs laid by each of the chickens to be tested and placing them in different test tubes, affixing the corresponding test tube labels on each of the test tubes, and placing the test tubes in a freezing box in sequence according to the serial number information on the test tube labels for freezing;

[0010] (5) Before testing, thaw the egg white in each test tube in the freezing box, and take the egg white in each test tube into different culture wells in the cell culture plate. The position order of the culture wells where the egg white in each test tube is located in the cell culture plate corresponds to the placement order of each test tube in the freezing box;

[0011] (6) performing an avian leukosis test on the egg white in each culture well in the cell culture plate, and filling or pasting the corresponding test results into or pasting the chicken information table according to the corresponding relationship between the position sequence of the culture wells where the egg white is located in the cell culture plate and the arrangement sequence of the chicken information in the chicken information table.

[0012] Preferably, the corresponding chicken information recorded in the QR code includes the chicken's strain, generation, birth batch, breeding cage number and wing number.

[0013] Preferably, the egg label also includes information on the strain and cage number of the corresponding chicken.

[0014] The egg labels can be prefabricated before testing, and a plurality of corresponding egg labels can be made for each chicken to be tested.

[0015] Preferably, in step (2), a number of eggs, such as 3 or 4 eggs, are collected from each chicken to be inspected.

[0016] Preferably, in step (2), the collected eggs are placed in egg trays, and it is ensured that several eggs laid by the same chicken to be inspected are placed together in the same egg tray, and multiple egg trays are placed in a stack, and each stack of egg trays contains the same number of eggs laid by the chickens to be inspected (i.e., the eggs contained in each stack of egg trays are laid by the same number of chickens to be inspected).

[0017] The staff of the testing laboratory can check the egg information according to the strain and cage number of the chicken in the egg label to ensure that several eggs laid by the same chicken to be tested are placed in the same egg tray.

[0018] Preferably, each stack of egg trays contains 92 eggs laid by the chickens to be inspected.

[0019] Preferably, in step (3), a barcode scanner is used to scan the QR code on any egg laid by each chicken to be inspected (i.e., only the QR code on one egg laid by each chicken to be inspected is scanned).

[0020] Preferably, a chicken information table (usually an EXCEL table) is generated for each stack of eggs in the egg tray.

[0021] Preferably, the serial number information on the test tube label includes a first serial number and a second serial number, the first serial number corresponds to the number of the egg tray where the eggs laid by the corresponding chicken are located, and the second serial number corresponds to the arrangement order of the eggs laid by the corresponding chicken in the corresponding egg tray.

[0022] Preferably, the test tube label at least includes the strain, breeding cage number and serial number information (including the first serial number and the second serial number) of the corresponding chicken.

[0023] Preferably, in step (4), the egg whites of all eggs laid by each chicken to be tested are collected and placed in the same test tube.

[0024] Preferably, in step (4), before taking the egg whites of the eggs laid by the chickens to be inspected, the eggshells of all the eggs are disinfected with 75% alcohol (75% alcohol by volume).

[0025] Preferably, the cell culture plate is a 96-well V-shaped plate.

[0026] Preferably, the corresponding chicken information table, freezing box and cell culture plate are named or numbered the same.

[0027] The beneficial effects of the present invention are as follows: the present invention combines information collection technology with traditional avian leukosis detection technology, adopts the method of attaching information labels on eggs and test tubes to replace the method of manually writing information in the traditional method, eliminates the errors that are easy to occur in the process of handwriting and copying information, and improves the accuracy of the information of the chickens to be tested and the test results. Compared with the traditional manual writing of information, the labeling method is faster and can effectively reduce the sampling time. During sampling, the same number of egg white samples of the chickens to be tested are stored in each freezing box. Before the test, the number of samples to be tested can be understood, which is convenient for arranging the test time reasonably and in time, and improving the test efficiency. Before the test, the information of the chickens to be tested is transmitted to the computer through the two-dimensional code information collection technology, and the order of the samples to be tested corresponds to the order of the information of the chickens to be tested input into the computer. Once the test results come out, the information of the chickens to be tested corresponding to each test result can be quickly known, which is convenient for timely elimination of positive chickens and reducing the probability of transmission. According to statistics, compared with traditional detection operation methods, the speed of collecting eggs laid by the chickens to be inspected can be increased by 39.82%, and the speed of entering the information of the chickens to be inspected into the computer can be increased by 67.46%. In large-scale detection (when there are more than 1,000 chickens to be inspected), the accuracy of the information of the chickens to be inspected and the test results can be increased to more than 99.84%. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0029] See also Figure 1 The present invention discloses an egg white avian leukosis detection operation method, comprising the following steps:

[0030] (1) preparing an egg label for each chicken to be inspected, wherein the egg label at least includes a QR code recording information of the corresponding chicken;

[0031] (2) collecting eggs laid by each chicken to be inspected, and attaching the corresponding egg label to each egg;

[0032] (3) Based on the QR code information conversion system, a barcode scanner is used to scan the QR code on the eggs laid by each chicken to be inspected to generate a chicken information table, and a test tube label corresponding to each chicken to be inspected is printed based on the chicken information in the chicken information table, wherein the test tube label at least includes serial number information corresponding to the arrangement order of the corresponding chicken information in the chicken information table;

[0033] (4) taking the egg whites of the eggs laid by each of the chickens to be tested and placing them in different test tubes, affixing the corresponding test tube labels on each of the test tubes, and placing the test tubes in a freezing box in sequence according to the serial number information on the test tube labels for freezing;

[0034] (5) Before testing, thaw the egg white in each test tube in the freezing box, and take the egg white in each test tube into different culture wells in the cell culture plate. The position order of the culture wells where the egg white in each test tube is located in the cell culture plate corresponds to the placement order of each test tube in the freezing box;

[0035] (6) performing an avian leukosis test on the egg white in each culture well in the cell culture plate, and filling or pasting the corresponding test results into or pasting the chicken information table according to the corresponding relationship between the position sequence of the culture wells where the egg white is located in the cell culture plate and the arrangement sequence of the chicken information in the chicken information table.

[0036] The corresponding chicken information recorded in the QR code preferably includes the chicken's strain, generation, birth batch, cage number and wing number, so that the information of the chicken to be inspected can be quickly and accurately transmitted to the computer through the QR code information collection technology. In addition to the QR code, the egg label also includes the corresponding chicken strain and cage number information, which is convenient for checking the information of the chicken to be inspected and the eggs it produces.

[0037] The egg labels can be prefabricated before testing to save time in the testing process and improve testing efficiency.

[0038] In step (2), preferably, several eggs, such as 3 or 4 eggs, are collected from each chicken to be inspected. A plurality of corresponding egg labels can be prepared for each chicken to be inspected, so as to be suitable for use with the several eggs collected from each chicken to be inspected. The egg label can be attached to the middle of the corresponding egg to facilitate observation of the information thereon.

[0039] In the step (2), the collected eggs are preferably placed in an egg tray, and it is ensured that several eggs laid by the same chicken to be inspected are placed in the same egg tray, which is convenient for egg white sampling in the subsequent detection process. Multiple egg trays are placed in a stack, and each stack of egg trays contains the same number of eggs laid by the chickens to be inspected (that is, the eggs contained in each stack of egg trays are laid by the same number of chickens to be inspected), which is convenient for information and quantity statistics of the chickens to be inspected.

[0040] The staff of the testing laboratory can check the egg information according to the strain and cage number of the chicken in the egg label to ensure that several eggs laid by the same chicken to be tested are placed in the same egg tray.

[0041] Each stack of egg trays preferably contains eggs laid by 92 chickens to be tested, so that egg white samples of the eggs contained in each stack of egg trays can be placed in the same cell culture plate (e.g., a 96-well plate). Preferably, a chicken information table (usually an EXCEL table) is generated for each stack of eggs contained in the egg trays to facilitate comparison of the test results with the information of the chickens to be tested in the chicken information table.

[0042] Since the information on the egg labels affixed to several eggs laid by the same chicken to be inspected is the same, in the step (3), a barcode scanner is used to scan the QR code on any egg laid by each chicken to be inspected, that is, only the QR code on one egg laid by each chicken to be inspected needs to be scanned.

[0043] The serial number information on the test tube label preferably includes a first serial number and a second serial number, wherein the first serial number corresponds to the number of the egg tray where the eggs laid by the corresponding chicken are located, and the second serial number corresponds to the arrangement order of the eggs laid by the corresponding chicken in the corresponding egg tray. The first serial number and the second serial number can be set when the information of the chicken to be inspected is entered into the computer (based on the QR code information conversion system, when the QR code on the eggs laid by each chicken to be inspected is scanned by a barcode scanner).

[0044] The test tube label preferably includes at least the strain, cage number and serial number information (including the first serial number and the second serial number) of the corresponding chicken, so as to facilitate information verification of the chicken to be tested and the egg white sampling of the eggs produced.

[0045] In the step (4), the egg whites of all the eggs laid by each chicken to be tested are collected and placed in the same test tube, so that the egg white sampled in each test tube corresponds to one chicken to be tested. Before taking the egg whites of the eggs laid by each chicken to be tested, the eggshells of all the eggs are disinfected with 75% alcohol (75% alcohol by volume) to avoid contamination during egg white sampling, which may affect the accuracy of the test results.

[0046] The cell culture plate preferably adopts a 96-well V-shaped plate so that one cell culture plate can hold egg white samples from a stack of eggs in an egg tray, so as to facilitate the comparison of subsequent test results with the information of the chickens to be tested in the chicken information table (each chicken information table corresponds to the information of the chickens to be tested in a stack of eggs in an egg tray).

[0047] The corresponding chicken information table, freezing box and cell culture plate preferably use the same name or number to facilitate information comparison and avoid errors.

[0048] A preferred implementation of the egg white avian leukosis detection operation method is:

[0049] (1) After the chickens to be tested are identified on site, the strain, generation, birth batch, cage number and wing number of the chickens to be tested are converted into a QR code in advance. The generated QR code and the strain and cage number of the corresponding chickens to be tested are designed on the same egg label, and the label is printed.

[0050] (2) Collect the eggs laid by each chicken to be inspected, and attach the egg label to the middle of the corresponding egg when collecting eggs. After collecting 3 eggs from the same chicken to be inspected, place them in an egg tray with the big end facing up. The egg tray can hold 30 eggs, and each egg tray holds 10 eggs laid by the chickens to be inspected. After the eggs are delivered to the laboratory, the laboratory staff will check the egg information according to the strain and breeding cage number on the egg label on the eggs to ensure that the 3 eggs from the same chicken to be inspected are placed in the same egg tray, and the egg trays holding eggs laid by 92 chickens to be inspected are placed in a stack.

[0051] (3) Open the QR code information conversion system, connect the wireless barcode scanner, set the first serial number and the second serial number in the system, scan the verified eggs with the barcode scanner, and scan the QR code in one egg for each chicken to be inspected. The first serial number represents the number of the egg tray stack where the eggs laid by the corresponding chicken to be inspected are located (the number of the stack), and the second serial number represents the arrangement order of the eggs laid by the corresponding chicken to be inspected in the corresponding egg tray stack (the number of the sample in the egg tray stack). To facilitate subsequent testing, the maximum setting value of the second serial number is 92. After scanning the egg QR code, the interface will display the strain, generation, birth batch, breeding cage number, wing number and serial number information.

[0052] After the information of every 92 chickens to be inspected is scanned and entered into the system, the relevant information is exported to an EXCEL table (the chicken information table) and named PX, where X is the same as the number of the first serial number. Click to open the label printer, print the strain, serial number and cage number information into the test tube label, and place it in the corresponding stack of egg trays after printing.

[0053] (4) After the information of the eggs is collected, the eggshell surface is first disinfected with 75% alcohol, and then the eggshell surface is broken with sterile tweezers to remove the eggshell. 300uL of egg white is absorbed from each egg and placed in a test tube (centrifuge tube). The egg whites of three eggs from the same chicken to be inspected are placed in the same test tube (i.e., 900uL of egg white is placed in each test tube). The corresponding second label is affixed to the corresponding test tube according to the information in the first label on the egg from which the egg white is collected, and the test tubes are placed in a 100-hole freezing box in the order of 1-92 and named PX, where X is the same as the number in the first serial number. Finally, they are stored in a sealed bag and placed in a refrigerator at -20°C or below for freezing and inspection.

[0054] (5) Before the test, the freezing box is taken out, the egg white is thawed and mixed evenly by shaking, and 150uL of egg white is pipetted from each test tube into a disposable 96-well V-shaped plate (the cell culture plate) (the position sequence of the culture wells where the egg white in each test tube is located in the 96-well V-shaped plate corresponds to the placement sequence of each test tube in the freezing box), and the disposable 96-well V-shaped plate is numbered according to the name on the freezing box (or the first serial number in the test tube label), and the egg white is used for subsequent avian leukosis detection.

[0055] (6) The egg white in each culture well of the 96-well V-shaped plate is tested for avian leukosis. After the test, the corresponding test result S / P value is pasted into the corresponding position in the EXCEL table (the corresponding position of the corresponding information of the chicken to be tested) according to the number of the 96-well V-shaped plate and the order of the test results, and the avian leukosis test results of each chicken to be tested can be presented.

[0056] Through experimental comparison, the egg white avian leukosis detection operation method of the present invention is compared with the traditional operation method:

[0057] (1) Time can be saved in the process of collecting eggs in the chicken house and entering the subsequent test results. The speed of collecting eggs in the chicken house can be increased from an average of 3 minutes and 41 seconds per 10 chickens using the traditional operation method to an average of 2 minutes and 13 seconds using the operation method of the present invention, an increase of 39.82%. The comparison results are shown in Table 1.

[0058] Table 1 Comparison of the speed of collecting eggs to be inspected in the shed using the traditional operation method and the operation method of the present invention

[0059] Group Manual writing mark (traditional operation method) Labeling (method of operation of the present invention) Repeat 1 3 minutes 36 seconds / 10 pieces 2 minutes 25 seconds / 10 pieces Repeat 2 3 minutes 27 seconds / 10 pieces 2 minutes 08 seconds / 10 pieces Repeat 3 3 minutes 47 seconds / 10 pieces 2 minutes 05 seconds / 10 pieces Repeat 4 3 minutes 55 seconds / 10 pieces 2 minutes 15 seconds / 10 pieces Average time 3 minutes 41 seconds / 10 pieces 2 minutes 13 seconds / 10 pieces

[0060] The information of the chickens to be inspected can be entered in advance before the inspection, and only one person is required to complete the entry. The time for entering the information on the eggs in each stack of egg trays is increased from an average of 9 minutes and 4 seconds using the traditional operation method to an average of 2 minutes and 57 seconds using the operation method of the present invention, which increases the speed by 67.46%. The comparison results are shown in Table 2.

[0061] Table 2 Comparison of the time for entering the information on the eggs in each egg tray using the conventional operation method and the operation method of the present invention

[0062] method Manual input (traditional operation method) Scan code to input (operation method of the present invention) Repeat 1 4 minutes 35 seconds / 2 people / 92 2 minutes 48 seconds / person / 92 Repeat 2 4 minutes 21 seconds / 2 people / 92 3 minutes and 5 seconds / person / 92 Repeat 3 4 minutes 40 seconds / 2 people / 92 2 minutes 58 seconds / person / 92 Average time 9 minutes and 4 seconds / person / 92 2 minutes 57 seconds / person / 92

[0063] In addition, by adopting the operation method of the present invention, the egg white can be stored in a freezing box in advance before the test, and the number of untested samples can be timely known, which is convenient for arranging the test time and reasonably arranging the test personnel on the test day, so as to maximize the test efficiency.

[0064] (2) The accuracy of the test results can be improved, especially in large-scale testing, the accuracy of the test results can be improved from 94.66% using the traditional operation method to more than 99.84% using the operation method of the present invention. The comparison results are shown in Table 3.

[0065] Table 3 Comparison of the accuracy of the test results using the traditional operation method and the operation method of the present invention

[0066]

[0067] It can be seen from the experimental comparison results that the egg white avian leukosis detection operation method of the present invention optimizes the egg white avian leukosis detection operation process compared with the traditional operation method, and can effectively improve the detection efficiency of egg white avian leukosis detection and the accuracy of the detection results.

Claims

1. Egg white avian leukosis detection operation method, characterized in that The following steps are involved: (1) preparing an egg label for each chicken to be inspected, wherein the egg label at least includes a QR code recording the corresponding chicken information; (2) Collecting eggs laid by each chicken to be inspected, and attaching the corresponding egg label to each egg; (3) Based on the QR code information conversion system, a barcode scanner is used to scan the QR code on the eggs laid by each chicken to be inspected to generate a chicken information table, and a test tube label corresponding to each chicken to be inspected is printed based on the chicken information in the chicken information table, wherein the test tube label at least includes serial number information corresponding to the arrangement order of the corresponding chicken information in the chicken information table; (4) taking the egg whites of the eggs laid by the chickens to be tested and placing them in different test tubes, affixing the corresponding test tube labels on the test tubes, and placing the test tubes in a freezing box in sequence according to the serial number information on the test tube labels for freezing; (5) Before testing, thaw the egg white in each test tube in the freezing box and place the egg white in each test tube in different culture wells in the cell culture plate. The position order of the culture wells where the egg white in each test tube is located in the cell culture plate corresponds to the order in which the test tubes are placed in the freezing box. (6) Performing an avian leukosis test on the egg white in each culture well of the cell culture plate, and filling or pasting the corresponding test results into the chicken information table according to the corresponding relationship between the position sequence of the culture wells where the egg white is located in the cell culture plate and the arrangement sequence of the chicken information in the chicken information table.

2. The egg white avian leukosis detection operation method as claimed in claim 1, characterized in that The corresponding chicken information recorded in the QR code includes the chicken's strain, generation, birth batch, breeding cage number and wing number.

3. The egg white avian leukosis detection operation method as claimed in claim 2, characterized in that The egg label also includes the strain and cage number information of the corresponding chicken.

4. The egg white avian leukosis detection operation method as claimed in claim 3, characterized in that In step (2), a number of eggs are collected from each chicken to be inspected.

5. The egg white avian leukosis detection operation method as claimed in claim 4, characterized in that In step (2), the collected eggs are placed in an egg tray, and it is ensured that several eggs laid by the same chicken to be inspected are placed together in the same egg tray, and multiple egg trays are placed in a stack, and each stack of egg trays contains the same number of eggs laid by the chicken to be inspected.

6. The egg white avian leukosis detection operation method as claimed in claim 5, characterized in that In the step (3), a barcode scanner is used to scan the QR code on any egg laid by each chicken to be inspected.

7. The egg white avian leukosis detection operation method as claimed in claim 6, characterized in that A chicken information table is generated for each stack of eggs placed in the egg tray.

8. The egg white avian leukosis detection operation method as claimed in claim 7, characterized in that The serial number information on the test tube label includes a first serial number and a second serial number, wherein the first serial number corresponds to the number of the egg tray where the eggs laid by the corresponding chicken are located, and the second serial number corresponds to the arrangement order of the eggs laid by the corresponding chicken in the corresponding egg tray.

9. The egg white avian leukosis detection method as claimed in claim 8, characterized in that In step (4), the egg whites of all eggs laid by each chicken to be tested are collected and placed in the same test tube.

10. The egg white avian leukosis detection operation method according to claim 9, characterized in that The corresponding chicken information sheets, freezing boxes and cell culture plates are named or numbered the same.

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