Low-temperature baked pet food composition for preventing and treating diarrhea of dogs and cats and application of low-temperature baked pet food composition

Abstract

The invention relates to the technical field of pet food, and discloses a low-temperature baked pet food composition for preventing and treating diarrhea of dogs and cats, the low-temperature baked pet food composition comprises a traditional Chinese medicine composition and a baked food basic nutritional composition, and the traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus, 7-10 parts of bighead atractylodes rhizome, 7-10 parts of Chinese yam, 6-8 parts of poria cocos, 6-8 parts of smoked plum and 6-8 parts of purslane. The basic nutritional composition for the baked food comprises the following components in parts by weight: 60-70 parts of dried chicken, 5-10 parts of chicken liver, 5-10 parts of chicken heart, 1-5 parts of potato powder, 1-5 parts of cassava starch, 1-5 parts of chicken oil, 1-5 parts of fish oil, 1-5 parts of chicken liver powder, 1-5 parts of krill powder, 1-5 parts of cellulose, 1-5 parts of fruit and vegetable powder and 1-5 parts of vitamin mineral substances. A traditional Chinese medicine composition is creatively combined with a basic nutritional composition of the baked food, and all the components have a synergistic effect, so that the baked food is suitable for being eaten by dogs and cats for a long time, meets all nutritional requirements of dogs and cats, and meanwhile, can obviously reduce diarrhea of dogs and cats and improve gastrointestinal immunity of dogs and cats.

Description

Technical Field

[0001] The invention relates to the technical field of pet food, and in particular to a low-temperature baked pet food composition and application thereof for preventing and treating diarrhea in dogs and cats. Background Art

[0002] Intestinal problems are one of the most common problems for pet dogs and cats. The main function of the intestine is to digest food and extract and absorb nutrients for the body. The digestive process relies on the power generated by the digestive tract and the participation of many substances and enzymes that help digestion. When food raw materials pass through the digestive tract, each part of the digestive tract performs specific and different digestive functions on it. When pet dogs and cats have intestinal problems, their digestion and absorption are hindered, affecting the absorption of nutrients, etc., threatening the health and even life of dogs and cats.

[0003] There are many factors that affect the intestinal function of pet dogs and cats: pathogenic factors, such as plague, rotavirus infection, coronavirus infection, ascariasis, coccidiosis, etc.; nutritional factors, incomplete, unbalanced, and poor-quality nutrition fed to dogs and cats, resulting in nutritional deficiencies in the body, which will directly affect the function of the intestinal mucosal immune system and the health of the gastrointestinal tract. For example, if there is a lack of vitamin B in the feed, pets will show anorexia, indigestion, diarrhea, etc., and a lack of vitamin D and an imbalance in the ratio of calcium and phosphorus can cause a decline in gastrointestinal function; poisoning factors, pets drink polluted water and accidentally eat moldy and spoiled food, which causes damage to the intestinal mucosal immune system and the digestion and absorption function of the gastrointestinal tract of pet dogs and cats; environmental factors, sudden changes in temperature, humidity and stuffiness, poor ventilation, sudden changes in the environment, etc., can easily cause dogs and cats to have a stress response, thereby changing the function of the gastrointestinal tract of dogs and cats, thereby endangering the health of the gastrointestinal tract and inducing various diseases.

[0004] At present, the main treatments for diarrhea in dogs and cats are antibiotics, physical adsorption, and probiotics / enzyme preparations. Antibiotics can cause intestinal flora disorders in dogs and cats, making them unable to fully recover; excessive addition of physical adsorbents can cause excessive ash content in the feces of dogs and cats. Probiotics or enzyme preparations are often used in pet food to regulate the intestinal health of dogs and cats. The vacuum spraying method after use ensures the activity of probiotics or enzyme preparations, which helps dogs and cats maintain the balance of their own intestinal flora, enhance their own immunity, and restore body functions to normal levels.

[0005] As pet owners love their pets and learn more about nutrition, the market demand for dog and cat food is growing, and the variety is increasing. Existing dog and cat food is filled with a lot of starch plant raw materials, which are not easy for dogs and cats to absorb and utilize, and only uses probiotics to improve the function of the intestinal digestive system.

[0006] Currently, most diets on the market that use probiotics to regulate intestinal health cannot avoid the problem of probiotic activity being affected by extrusion, high temperature, etc. during food processing. When the activity of probiotics decreases, the improvement of intestinal health of dogs and cats by probiotic diets will be greatly reduced. In addition, some probiotic products only have a certain effect during use. Once discontinued, pets' intestinal problems will recur, causing negative impacts on the body's intestinal immunity. Therefore, the market needs dog and cat food that is closer to the original diet of dogs and cats and can improve intestinal immunity in multiple dimensions. Summary of the invention

[0007] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats.

[0008] To achieve the above objectives, in a first aspect, the present invention provides a low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats, comprising a Chinese medicine composition and a baked grain basic nutritional composition.

[0009] Furthermore, the Chinese medicine composition is made of the following components: Astragalus, Atractylodes macrocephala, Chinese yam, Poria cocos, Portulaca oleracea, and Prunus mume. Atractylodes macrocephala strengthens the spleen and dries dampness, and is the main medicine; Chinese yam strengthens the spleen and kidneys, assists Atractylodes macrocephala in restoring gastrointestinal function, and is the minister medicine; Astragalus strengthens the spleen and stomach, astringes the intestines and stops diarrhea, Poria cocos strengthens the spleen and eliminates dampness, and Astragalus and Poria cocos are both adjuvants; Prunus mume astringes and protects the intestinal mucosa, Portulaca oleracea clears heat and detoxifies, and clears dampness and heat in the intestines, and Prunus mume and Portulaca oleracea are both guiding medicines. The combination of these medicines can achieve the effects of strengthening the spleen and drying dampness, astringing the intestines and stopping diarrhea, astringing and stopping bleeding, and clearing heat and detoxifying. By regulating the spleen and stomach function, promoting gastrointestinal digestion, and repairing gastrointestinal damage, the diarrhea of ​​dogs and cats can be improved, and at the same time, appetite can be promoted and solid and healthy feces can be discharged.

[0010] The weight proportions of the components of the traditional Chinese medicine composition of the present invention are 10-20 parts of astragalus, 7-10 parts of atractylodes, 7-10 parts of yam, 6-8 parts of tuckahoe, 6-8 parts of ebony and 6-8 parts of purslane.

[0011] Preferably, the Chinese medicine composition of the present invention is prepared by the following formula of components in parts by weight: 10-15 parts of Astragalus, 6-8 parts of Atractylodes, 6-8 parts of Dioscorea, 5-7 parts of Poria, 5-7 parts of Prunus mume, and 5-7 parts of Portulaca oleracea.

[0012] Further preferably, the weight proportions of the components of the traditional Chinese medicine composition of the present invention are 10 parts of Astragalus, 7 parts of Atractylodes, 7 parts of Dioscorea, 6 parts of Poria, 6 parts of Ebony Plum, and 6 parts of Portulaca Oleracea.

[0013] The weight proportions of the components of the baked grain basic nutritional composition of the present invention are as follows: 60-70 parts of dried chicken, 5-10 parts of chicken liver, 5-10 parts of chicken heart, 1-5 parts of potato flour, 1-5 parts of cassava starch, 1-5 parts of chicken fat, 1-5 parts of fish oil, 1-5 parts of chicken liver powder, 1-5 parts of krill powder, 1-5 parts of cellulose, 1-5 parts of fruit and vegetable powder, and 1-5 parts of vitamins and minerals.

[0014] Preferably, the vitamins and minerals include vitamin A acetate, vitamin D, dl-α-tocopherol acetate, thiamine nitrate, vitamin B2, vitamin B6, D-calcium pantothenate, niacinamide, antioxidants, folic acid, D-biotin, potassium chloride, calcium hydrogen phosphate, protein iron, protein copper, protein manganese, protein zinc, potassium iodide, yeast selenium, and sodium chloride.

[0015] Preferably, the fruit and vegetable powder includes carrot, parsley, chicory root, tomato and pear.

[0016] The low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats of the present invention comprises a Chinese medicine composition having a weight ratio of 0.5%-2% and a baked grain basic nutritional composition having a weight ratio of 98%-99.5%.

[0017] The low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats of the present invention is prepared by the following method:

[0018] (1) Raw material processing: crushing and mixing the chicken breast, chicken liver and chicken heart, adding enzyme preparation, performing enzymolysis at a temperature of 40-60° C. for 1-4 hours, and temporarily storing after enzymolysis to obtain enzymolysis meat slurry; the enzyme preparation includes protease, amylase, cellulase and β-glucanase;

[0019] (2) Preliminarily mixing the potato flour, cassava starch, cellulose, krill meal, fruit and vegetable powder, Chinese medicine composition and vitamins and minerals in a mixer for 1-5 minutes, and then performing a second mixing with the enzymatically hydrolyzed meat slurry, dried chicken and fat in a mixer for 1-5 minutes;

[0020] (3) forming the second mixed raw material into a specific shape using a mold, and feeding the raw material into a conveyor belt and passing through a baking and dehydration device for baking and dehydration to obtain baked and dehydrated dog and cat food;

[0021] (4) cooling the baked and dehydrated product, and then passing the cooled product through a metal detector to remove metals mixed in the dog and cat food;

[0022] (5) The dog and cat food that has passed the metal detector is sampled and tested, and the low-temperature baked pet food composition for preventing and treating dog and cat diarrhea that meets the quality requirements is obtained after the test is qualified.

[0023] In a second aspect, the present invention discloses the use of the Chinese medicine composition described in the first aspect in the preparation of a pet product for preventing and treating diarrhea.

[0024] In a third aspect, the present invention discloses a pet product for preventing and treating pet diarrhea, comprising the Chinese medicine composition described in the first aspect.

[0025] Compared with the prior art, the present invention provides a low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats, which has the following beneficial effects:

[0026] The present invention creatively combines a traditional Chinese medicine composition with a baked grain basic nutritional composition to form a low-temperature baked pet food composition that can prevent and treat diarrhea in dogs and cats. Through the synergistic effect of the components, on the one hand, it is suitable for long-term consumption by dogs and cats, meets all the nutritional needs of dogs and cats, and ensures the nutrition of dogs and cats; on the other hand, it can significantly reduce diarrhea in dogs and cats and improve the gastrointestinal immunity of dogs and cats. The present invention provides the market with a dog and cat food that has a composition formula that is closer to the original diet of dogs and cats and can improve the intestinal immunity of dogs and cats in multiple dimensions. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 This is a graph of cytokine analysis of intestinal tissue in Example 1.

[0029] Figure 2 This is a diagram of the colon pathological histological detection and analysis in Example 1.

[0030] Figure 3 This is a graph showing changes in intestinal microbial α diversity in Example 1.

[0031] Figure 4 This is a diagram of the analysis of the mouse intestinal microbial phylum level in Example 1.

[0032] Figure 5 This is a genus-level analysis diagram of the mouse intestinal microorganisms in Example 1.

[0033] Figure 6 This is a graph showing changes in cat food intake in Example 3.

[0034] Figure 7 This is a graph showing changes in dog food intake in Example 3.

[0035] Figure 8The following are the changes in the intestinal microecology of the cat in Example 3 over 10 days.

[0036] Fig. 9 The following are the changes in the intestinal microecology of the dogs in Example 3 over 10 days.

[0037] Fig.10 The figure shows the changes in the level of sIgA antibodies in the cat intestine in Example 3.

[0038] Fig.11 The figure shows the changes in the levels of sIgA antibodies in the canine intestine in Example 3. DETAILED DESCRIPTION

[0039] The following examples are provided for a better understanding of the present invention, but are not intended to limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are purchased from conventional biochemical reagent companies unless otherwise specified. The quantitative tests in the following examples are repeated three times, and the results are averaged.

[0040] Example 1: Screening of Chinese medicine compositions

[0041] 1. Formula of Chinese medicine composition

[0042] Formula of Chinese medicine composition 1: 10 parts of astragalus, 7 parts of atractylodes, 7 parts of yam, and 6 parts of purslane.

[0043] Formula of Chinese medicine composition 2: 10 parts of Astragalus, 7 parts of Chinese yam, 6 parts of Poria, 6 parts of Portulaca oleracea, 6 parts of Black Plum, and 6 parts of Alisma.

[0044] Formula of Chinese medicine composition 3: 10 parts of Astragalus, 7 parts of Atractylodes, 7 parts of Dioscorea, 6 parts of Poria, 6 parts of Portulaca Oleracea, and 6 parts of Ebony Plum.

[0045] 2. Animal Experiment Design

[0046] (1) Selection of mice: 6-week-old SPF-grade C57B6 / J mice were purchased from the Center for Comparative Medicine of Yangzhou University.

[0047] (2) Grouping of mice: The mice were randomly divided into 5 groups, namely, a blank group, a model group, a Chinese medicine composition formula 1 group, a Chinese medicine composition formula 2 group, and a Chinese medicine composition formula 3 group.

[0048] (3) Establishment of acute intestinal mucosal injury model in mice: After grouping, mice in each group were kept in an environment with a temperature of 25°C and a relative humidity of 40-60%, and were given free access to food and water. The bedding was changed daily. The mice were adaptively raised for 1 week, and then the experiment was carried out, as follows:

[0049] The mice in the blank group were given pure water to drink freely, and the other five groups were given 3% dextran sulfate sodium (DSS) solution to drink freely for 7 consecutive days to establish the mouse acute intestinal mucosal injury model.

[0050] For the mice in the Chinese medicine composition formula 1 group, the Chinese medicine composition formula 2 group and the Chinese medicine composition formula 3 group, each was gavaged with a dose of 200 mg / kg of the Chinese medicine composition of the corresponding formula, and fed continuously for 14 days, once a day, 0.3 mL each time. For the mice in the blank group and the model group, they were fed normally. After the feeding, the colon tissue of the mice was sampled and analyzed.

[0051] 3. Sample processing and data analysis

[0052] (1) Determination of cytokine levels in intestinal tissue

[0053] Place the colon tissue fragments collected in a 2mL EP tube, add several glass grinding beads treated without enzymes and 1mL PBS, place in an automatic homogenizer for homogenization for 20 minutes, and take out the supernatant and place in -20℃ for later use. During the determination, first use the BCA protein determination kit to determine the sample protein concentration, use PBS to balance according to the protein concentration, and then determine the cytokine level in the sample according to the instructions of different ELISA kits.

[0054] (2) Colon pathological histology

[0055] 0.5 cm of mouse colon was obtained, fixed with 4% paraformaldehyde and sent to the company for preparation into paraffin sections, which were then stained with hematoxylin and eosin to prepare H&E stained sections; AB-PAS stained sections were prepared using Alcian blue staining solution, 0.5% periodic acid, Schiff Reagent, hematoxylin staining solution and ammonia water; and fluorescent sections were prepared using DAPI, ZO-1 and Occludin fluorescent dyes.

[0056] (3) Intestinal microbial 16s rRNA sequencing

[0057] The integrity of the extracted genomic DNA was evaluated by agarose gel electrophoresis, and the quality of the DNA was tested using a Nanodrop 2000 instrument to ensure that the DNA concentration was above 20 ng / μL, the total amount was not less than 500 ng, and the OD260 / 280 value was between 1.8 and 2.0.

[0058] Genomic DNA is amplified using high-assurance PCR, and the amplified products are detected by agarose gel electrophoresis, and then purified using nucleic acid purification magnetic beads to construct the initial library of each sample. Further, these sample libraries with specific index tags are quantified and mixed. First, based on the preliminary quantitative results of agarose gel electrophoresis, the library concentration is appropriately diluted. Then, the library concentration is accurately measured using the Qubit precision instrument, and the samples are mixed according to a certain molar ratio according to the sequencing throughput required for different samples.

[0059] The library of the mixed sample was quality tested using the Agilent 2100 Bioanalyzer to check the size of the insert fragment in the sequencing library to ensure that the size was between 120 and 200 base pairs and to confirm that there was no nonspecific amplification. Finally, the library was sequenced at 2×250bp using the MiSeq platform. After sequencing, the data was analyzed by bioinformatics based on the R language.

[0060] The experimental data were analyzed by variance analysis and Duncan multiple comparison using Graphpad prism 9.1 software, and bioinformatics analysis was performed using R studio software. P<0.05 indicates a significant difference, which is statistically significant.

[0061] 4. Test results

[0062] (1) Chinese medicine combination formula can inhibit intestinal inflammation

[0063] The levels of proinflammatory cytokines IL-6, IL-1β, TNF-α, and IL-17A in mouse colon tissue were determined by ELISA. Figure 1 As shown, the three different Chinese medicine combination formulas can significantly inhibit (P<0.05) the increase in IL-6, IL-1β, TNF-α and IL-17A levels in colon tissue caused by intestinal mucosal damage, and for IL-6, the anti-inflammatory effect of Chinese medicine composition 3 is significantly better than that of formula 1 and formula 2 (P<0.05), and for IL-1β and TNF-α, the anti-inflammatory effect of Chinese medicine composition 3 is significantly better than that of formula 1 (P<0.05). The above results show that Chinese medicine compositions 1, 2, and 3 can significantly inhibit intestinal inflammation in mice, and Chinese medicine composition group 3 has the best effect.

[0064] (2) Chinese medicine combination formula can maintain the integrity of the intestinal barrier

[0065] ①Pathological histological test results:

[0066] H&E staining was used to examine the morphological structure of mouse colon tissue. Figure 2As shown in module A in the figure, in the modeling group, the inherent structure of the mouse colon tissue was destroyed, the mucosal epithelial cells and the cells of the lamina propria underwent severe necrosis, the crypt structure disappeared, and a large number of inflammatory immune cells gathered. In the treatment groups of the Chinese medicine composition 1, 2, and 3, the colon tissue maintained its inherent morphological structure and there was no obvious inflammatory cell infiltration. AB-PAS staining was used to detect the level of goblet cells in the mouse colon tissue, as shown in FIG. Figure 2 As shown in module B, in the modeling group, the number of goblet cells was significantly decreased, while in the treatment group, the number of goblet cells was significantly increased, and the number of goblet cells in the Chinese medicine composition 3 was more than that in the other two groups (P<0.05). The above results indicate that the Chinese medicine composition can maintain the integrity of the intestinal tissue structure of mice, increase the number of goblet cells in the intestinal tissue, and the effect of the Chinese medicine composition 3 is better than that of the other two Chinese medicine composition groups.

[0067] ②Immunofluorescence test results:

[0068] Immunofluorescence was used to detect the protein expression levels of tight junction proteins ZO-1 and Occludin in intestinal tissues. Figure 3 As shown in the figure, the green fluorescence of Occludin in the colon tissue of the modeling group was significantly dimmed. The fluorescence intensity was quantitatively analyzed by ImageJ software, and the fluorescence intensity of the model group was significantly reduced. The treatment of the Chinese medicine combination formula can restore the level of Occludin, but only the Chinese medicine combination formula 3 has a significant difference (P<0.05). Observing the red fluorescence representing ZO-1, it can also be seen that the Chinese medicine combination formula 3 has a trend of increasing the fluorescence intensity of ZO-1. The above results show that the Chinese medicine combination formula 3 can increase the level of tight junction proteins in colon tissue and has a protective effect on the intestinal barrier.

[0069] (3) Chinese medicine combination formula can improve intestinal microbial disorders

[0070] The above experiments showed that the anti-inflammatory and intestinal barrier maintenance effects of Chinese medicine composition 3 were better than those of the other two groups. Therefore, the focus was on studying the effects of Chinese medicine composition 3 on the intestinal microorganisms of mice to clarify its mechanism of regulating intestinal health.

[0071] ① Chinese medicine composition 3 formula can increase the diversity of intestinal microorganisms

[0072] The intestinal microorganisms of mice fed with Chinese medicine composition 3 were sequenced by 16sRNA. Figure 3 As shown, compared with the blank group, the number of operational classification units (ASV) in the model group was significantly decreased (P<0.05), while compared with the model group, the number of ASV in the formula of Chinese medicine composition 3 was significantly (P<0.05) increased.

[0073] The α-diversity analysis used Chao1 and ACE indices to represent the total number of species in the sample. A higher index indicates a greater number of species. The Shannon index was used to assess the species richness in the sample. An increase in the index indicates an increase in species richness. Figure 3 B, 3C, and 3D indicate that Chao1, ACE, and Shannon in the model group were significantly decreased compared with those in the blank group (P<0.05). After treatment with the Chinese medicine composition 3, the decreasing trend of microbial diversity was significantly reversed (P<0.05). The above results indicate that the Chinese medicine composition 3 can increase the diversity of intestinal microorganisms.

[0074] ② Chinese medicine composition 3 formula can improve intestinal microbial disorders

[0075] like Figure 4 As shown, the composition of intestinal microorganisms in mice was analyzed at the phylum level to evaluate the ability of Chinese medicine composition 3 to regulate intestinal microorganisms. The results showed that at the phylum level, compared with the blank group, the proportion of Firmicutes was significantly increased in the model group (P<0.05), the level of Bacteroidetes was significantly decreased (P<0.05), and the ratio of the two (F / B) was significantly increased (P<0.05), which means that the intestinal microbial structure is disordered and the animal body is in an unhealthy state. After treatment with Chinese medicine composition 3, the level of Bacteroidetes was significantly increased, and the level of F / B was significantly decreased (P<0.05), which shows that Chinese medicine composition 3 can regulate the intestinal microbial imbalance of mice at the phylum level.

[0076] like Figure 5 As shown in the results, the changes in intestinal microorganisms were analyzed in the water samples. Compared with the blank group, the proportion of harmful bacteria (Lombella and Turicibacter) in the modeling group increased significantly (P<0.05), and the proportion of beneficial bacteria such as Faecalibacterium, Prevotella, and Alloprevotella decreased, indicating that the microbial composition in the intestine was disordered after modeling. After treatment with Chinese medicine composition formula 3, the proportion of Faecalibacterium and Lactobacillus increased significantly (P<0.05), and the proportion of harmful bacteria (Lombella and Turicibacter) also decreased significantly (P<0.05). The above results show that Chinese medicine composition formula 3 can regulate the level of intestinal microorganisms in the water samples, improve intestinal microbial disorders, increase the proportion of beneficial microorganisms in the intestine, and reduce the proportion of harmful microorganisms, thereby protecting the intestine of the animal body.

[0077] Example 2: Preparation and effect analysis of low-temperature baked food for preventing and treating diarrhea in dogs and cats

[0078] 1. Formula of Chinese medicine composition: 10 parts of Astragalus, 7 parts of Atractylodes macrocephala, 7 parts of Dioscorea opposita, 6 parts of Poria, 6 parts of Portulaca oleracea, and 6 parts of Ebony plum.

[0079] 2. The dog food formula is shown in Table 1; the cat food formula is shown in Table 2.

[0080] Table 1

[0081] Puffed Dog Baking Dog 1 Baking Dog 2 chicken breast 15 50 50 Chicken Jerky 25 15 15 Chicken liver 8 9 8 Chicken Heart 7 7 7 Potato flour 17 4 4 Tapioca starch 13 2 2 Chicken fat 6 6 6 fish oil 2 2 2 Chicken Liver Powder 2 3 3 Krill Meal 0 0 0 Cellulose 2 1 1 Fruit and vegetable powder 2 0.9 0.5 Chinese medicine composition 1 0 1 Vitamins and Minerals 1 0.1 1.5

[0082] Table 2

[0083]

[0084]

[0085] Vitamin mineral compound premix contains per kg: Vitamin A acetate 600000IU-30000IU, Vitamin D3 60000IU-200000IU, Vitamin E 5000IU-20000IU, Vitamin B1 300-2000mg, Vitamin B2 300-2000mg, Vitamin B6 200-2000mg, Vitamin B12 3-30mg, biotin 10-100mg, pantothenic acid 500-3000mg, folic acid 50-200mg, niacinamide 1500-4000mg, iron 5000-20000mg, copper 500-2000mg, manganese 1000-6000mg, zinc 10000-30000mg, iodine 100-400, selenium 10-30mg, choline 30000-80000mg and taurine 100g-300g.

[0086] 3. Preparation of low-temperature baked dry food for dogs and cats. The specific operation method is as follows:

[0087] (1) Raw material processing: crushing and mixing the chicken breast, chicken liver and chicken heart, adding enzyme preparation, performing enzymolysis at a temperature of 40-60 degrees Celsius for 1-4 hours, and temporarily storing after enzymolysis to obtain enzymolysis meat slurry;

[0088] (2) Preliminarily mixing the potato flour, cassava starch, cellulose, krill meal, fruit and vegetable powder, Chinese medicine composition and vitamins and minerals in a mixer for 1-5 minutes, and then performing a second mixing with the enzymatically hydrolyzed meat slurry, dried chicken and fat in a mixer for 1-5 minutes;

[0089] (3) The second mixed raw material is molded into a specific shape by a mold, and enters a conveyor belt to pass through a baking and dehydration device for baking and dehydration. The baking and dehydration is divided into four stages. The temperature of the first two stages of baking and dehydration is 70-90 degrees Celsius, and the temperature of the last two stages of baking and dehydration is 60-80 degrees Celsius. The baking and dehydration time is 20-30 minutes to obtain a baked and dehydrated product. The moisture content of the baked and dehydrated product is 3%-8%;

[0090] (4) Cooling the baked and dehydrated product, the temperature of the cooled product does not exceed 5 degrees Celsius from room temperature, and the cooled product is tested by a metal detector to remove metal mixed in the product;

[0091] (5) Products that have passed through metal detectors are randomly inspected and packaged and stored after passing the inspection.

[0092] 4. Test the nutritional index of the low-temperature baked dog and cat dry food

[0093] (1) The nutritional index of dog food is shown in Table 3

[0094] Table 3

[0095]

[0096]

[0097] (2) The nutritional indicators of cat food are shown in Table 4

[0098] Table 4

[0099]

[0100]

[0101] 5. Cat and dog feeding test and result analysis

[0102] The hardness and crispness of the dry pet food prepared in the above examples were tested by a Brookfield CTX texture analyzer. The oil content on the surface of the food was evaluated by an oil infiltration test. The retention rates of heat-sensitive nutrients such as vitamin B1, vitamin B2, vitamin A, vitamin K3, vitamin B6, niacinamide, and D-biotin in the dry food particles prepared by the puffing and baking processes were evaluated by physical and chemical tests.

[0103] In addition, 20 sterilized dogs (9 female dogs and 11 male dogs) and 20 sterilized cats (9 female cats and 11 male cats) were selected for feeding experiments. They were aged 1-3 years old and randomly divided into 4 groups. Dogs were fed at 9:30 am and 4:30 pm every day. Cats were fed at 3:30 pm every day, and the food bowls were taken out at 9 am the next day. During this period, cats were free to eat. Dogs and cats were fed in kennels and catteries respectively. They were free to move around in the catteries every day. The indoor temperature was maintained at 20-25 degrees Celsius. The body weight was measured at 0, 1, 2, 3, and 4 weeks. During the test, the dogs and cats were observed for abnormal reactions.

[0104] The changes in body weight are shown in Tables 5 and 6:

[0105] Table 5

[0106]

[0107]

[0108] Table 6

[0109] Puffed cat food Baked cat food1 Baked cat food 2 Original weight, kg 4.2 4.5 4.1 Body weight after 4 weeks, kg 4.2 4.4 4.2 Weight change, kg 0 -0.1 +0.1 Daily feed intake, g 59.7 63.4 60.3

[0110] After feeding for 28 days, compared with the puffed food, there were no abnormal changes in the weight, food intake and feces of the dogs in each group of baked food, indicating that the baked food in this embodiment can meet the daily nutritional needs of dogs and cats.

[0111] The results of the dog food crispness test are shown in Table 7:

[0112] Table 7

[0113] food Thickness(mm) Hardness(g) Brittleness (peak number) Extruded dog food 4.89 3513 1 Baked dog food1 4.91 1823 5 Baked dog food 2 4.89 1840 4

[0114] The results of the cat food crispness test are shown in Table 8:

[0115] Table 8

[0116] food Thickness(mm) Hardness(g) Brittleness (peak number) Puffed cat food 3.81 3140 2 Baked cat food1 3.75 1429 8 Baked cat food 2 3.79 1431 7

[0117] The hardness and crispness of puffed and baked dog and cat food were tested by Brookfield CTX Texture Analyzer. The results showed that the hardness of baked pet dry food was lower than that of puffed pet dry food. The more peaks, the better the crispness of the dry food. The results showed that the crispness of baked pet dry food was better than that of puffed pet dry food.

[0118] Grease infiltration test:

[0119] The puffed and baked dog and cat dry food were evenly mixed in equal amounts of 1:1. 20g of puffed dog and cat food and 20g of baked dog and cat food were taken and placed on a white A4 paper for 20 minutes. The oil immersion on the paper was observed and the number and diameter of oil halos were counted. The results showed that a large number of obvious oil halos appeared in the puffed dry food, while a small number of slight oil halos appeared in the baked food. The diameter of the oil halo of the puffed dry food was larger than that of the baked food, and the surface of the puffed food was more greasy, as shown in Table 9.

[0120] Table 9

[0121]

[0122] The retention rate of heat-sensitive nutrients in dog food is shown in Table 10:

[0123] Table 10

[0124] Extruded dog food Baked dog food1 Baked dog food 2 Vitamin B1 60 85 87 Vitamin B2 85 90 90.2 Vitamin A 75 86 89 Vitamin B6 75 84 85 Niacinamide 90 93 93 D-Biotin 85 92 95

[0125] The retention rate of heat-sensitive nutrients in cat food is shown in Table 11:

[0126] Table 11

[0127] Puffed cat food Baked cat food1 Baked cat food 2 Vitamin B1 61 84 85 Vitamin B2 83 89 90 Vitamin A 77 85 86 Vitamin B6 74 83 85 Niacinamide 89 91 93 D-Biotin 84 91 92

[0128] It can be seen from this that the retention rate of heat-sensitive nutrients in baked dog and cat food is higher than that in puffed dog and cat food.

[0129] Example 3: Clinical application of the composition

[0130] 1. Screening of experimental animals:

[0131] 10 cats and 10 dogs were selected and divided into two groups for baking food test. The test period was 10 days. During the test, the dogs and cats were free to drink water. There was one test group with baked food containing Chinese medicine composition ingredients, and two test groups without baked food containing Chinese medicine composition ingredients.

[0132] The formula of the Chinese medicine composition is as follows: 10 parts of astragalus, 7 parts of atractylodes, 7 parts of yam, 6 parts of tuckahoe, 6 parts of purslane, and 6 parts of ebony.

[0133] 2. Experimental design

[0134] The information and feeding amount of the test dogs and cats are shown in Table 12 and Table 13

[0135] Table 12

[0136]

[0137]

[0138] Table 13

[0139]

[0140]

[0141] 3. Test results

[0142] (1) Analysis of feed intake trend

[0143] The results are as follows Figure 6 and Figure 7 As shown, adding the Chinese medicine composition has no effect on the food intake of dogs and cats, and does not affect the palatability of baked food.

[0144] (2) Analysis of stool status

[0145] The feces volume of the test cats and dogs was collected for 7 days (excluding the transition period), and the feces status and score were analyzed.

[0146] The scoring criteria and rules are as follows:

[0147]

[0148] The results are shown in Tables 14 and 15. The fecal scores of cats in the group supplemented with the Chinese medicine formula were better than those in the basic baked food group. Therefore, adding Chinese medicine formula 1 to the baked food can help improve soft stool diarrhea in dogs and cats and help form stool.

[0149] Table 14

[0150]

[0151] Table 15

[0152]

[0153] (3) Analysis of apparent digestibility of dry matter and protein digestibility

[0154] Fecal sample collection: Cat feces were collected and scored every day. The feces on the 6th to 10th day were collected, the cat litter was removed, and the wet weight was weighed. The feces were placed in a drying oven at 75°C for 24 hours and the dry weight was weighed. The fecal samples were then arranged by date and cat number, and crushed into fine powder in a wall breaker.

[0155] ① Calculation rules and analysis methods for average fecal protein content

[0156] Kjeldahl method for determination of crude protein in feces

[0157] ② Calculation rules and analysis methods of protein digestibility

[0158] Calculation formula:

[0159] Protein digestibility (crude protein dry basis):

[0160] (Feed intake*Crude protein in grain*(1-water)-(Dry weight of feces*Feces protein content))

[0161] ((Feed intake*Crude protein in grain*(1-water content))

[0162] ③Calculation rules and analysis methods of dry matter digestibility

[0163] Apparent digestibility of dry matter:

[0164] (Feed intake*(1-water content)-total dry weight of feces))

[0165] (Feed intake*(1-water content)

[0166] Table 16

[0167]

[0168] Table 17

[0169]

[0170] As shown in Tables 16 and 17, the average protein content of group 1 for dogs and cats was lower than that of group 2, and the average protein digestibility and dry matter digestibility were higher than those of group 2. The experimental data results show that adding Chinese medicine formula to baked food is more beneficial to the digestion and absorption of dogs and cats.

[0171] (4) Analysis of changes in intestinal flora

[0172] Fresh feces of dogs and cats were collected (the uncontaminated part of the feces must be taken), and then stored at -80℃. After all the feces were collected at the end of the experiment, 16s rDNA sequencing was performed to analyze the changes in the intestinal microecology of the experimental dogs and cats.

[0173] Experimental results: Changes in intestinal flora Figure 8 and Fig. 9 Compared with the 0 day, the ratio of thick wall / pseudo-rod in dog bean curd was significantly reduced after 10 days of intervention, and the phyla of Deformation, Actinobacterium, and Fusobacterium decreased; after 10 days of intervention with cat two blue, except for Lactobacillus, Faecalibacterium, Fusobacterium, Blautia, and Escherichia-Shigella increased to varying degrees.

[0174] Intestinal microecological tests on dogs and cats show that Chinese herbal medicine formulas can significantly increase beneficial intestinal bacteria and significantly reduce harmful bacteria, which helps maintain the balance of intestinal flora and keep the intestines healthy.

[0175] (5) Changes in fecal secretory immunoglobulin A antibody levels

[0176] Fresh feces of dogs and cats were collected (the uncontaminated part of the feces must be taken), and then stored at -80℃. After the experiment was over, all the feces were collected and the sIgA antibody levels were tested to analyze the changes in antibody levels of the experimental dogs and cats.

[0177] Fecal secretory immunoglobulin A antibody levels Fig.10 and Fig.11 As shown in Figure 2, the secretory immunoglobulin A of dogs and cats showed an upward trend.

[0178] Therefore, adding the Chinese medicine composition to the baked food in this embodiment can improve intestinal immunity.

[0179] Finally, it should be noted that the above invention is only a preferred embodiment of the invention and is not intended to limit the invention. Although the invention is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the invention shall be included in the protection scope of the invention.

Claims

1. A low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats, characterized in that: It includes a traditional Chinese medicine composition and a baked grain basic nutritional composition.

2. The food composition according to claim 1, characterized in that The Chinese medicine composition is prepared from the following components: astragalus, atractylodes, yam, poria, purslane, and black plum; the weight proportions of each component are 10-20 parts of astragalus, 7-10 parts of atractylodes, 7-10 parts of yam, 6-8 parts of poria, 6-8 parts of black plum, and 6-8 parts of purslane.

3. The food composition according to claim 2, characterized in that The traditional Chinese medicine composition is prepared by the following formula of components in parts by weight: 10-15 parts of astragalus, 6-8 parts of atractylodes, 6-8 parts of yam, 5-7 parts of tuckahoe, 5-7 parts of ebony, and 5-7 parts of purslane.

4. The food composition according to claim 2, characterized in that The weight proportions of the components of the traditional Chinese medicine composition are 10 parts of astragalus, 7 parts of atractylodes, 7 parts of yam, 6 parts of tuckahoe, 6 parts of ebony and 6 parts of purslane.

5. The food composition according to claim 1, characterized in that The weight proportions of the components of the baked grain basic nutritional composition are as follows: 60-70 parts of dried chicken, 5-10 parts of chicken liver, 5-10 parts of chicken heart, 1-5 parts of potato flour, 1-5 parts of cassava starch, 1-5 parts of chicken fat, 1-5 parts of fish oil, 1-5 parts of chicken liver powder, 1-5 parts of krill powder, 1-5 parts of cellulose, 1-5 parts of fruit and vegetable powder, and 1-5 parts of vitamins and minerals.

6. The food composition according to claim 5, characterized in that The vitamins and minerals include vitamin A acetate, vitamin D, dl-α-tocopherol acetate, thiamine nitrate, vitamin B2, vitamin B6, D-calcium pantothenate, niacinamide, antioxidants, folic acid, D-biotin, potassium chloride, calcium hydrogen phosphate, protein iron, protein copper, protein manganese, protein zinc, potassium iodide, yeast selenium, and sodium chloride; the fruit and vegetable powder includes carrots, parsley, chicory root, tomatoes, and pears.

7. The food composition according to claim 1, characterized in that The weight ratio of the traditional Chinese medicine composition is 0.5%-2%, and the weight ratio of the baked grain basic nutritional composition is 98%-99.5%.

8. The low-temperature baked pet food composition for preventing and treating diarrhea in dogs and cats according to claim 1, characterized in that: It is prepared by the following method: (1) Raw material processing: crushing and mixing the chicken breast, chicken liver and chicken heart, adding enzyme preparation, performing enzymolysis at a temperature of 40-60° C. for 1-4 hours, and temporarily storing after enzymolysis to obtain enzymolysis meat slurry; the enzyme preparation includes protease, amylase, cellulase and β-glucanase; (2) Preliminarily mixing the potato flour, cassava starch, cellulose, krill meal, fruit and vegetable powder, Chinese medicine composition and vitamins and minerals in a mixer for 1-5 minutes, and then performing a second mixing with the enzymatically hydrolyzed meat slurry, dried chicken and fat in a mixer for 1-5 minutes; (3) forming the second mixed raw material into a specific shape using a mold, and feeding the raw material into a conveyor belt and passing through a baking and dehydration device for baking and dehydration to obtain baked and dehydrated dog and cat food; (4) cooling the baked and dehydrated product, and then passing the cooled product through a metal detector to remove metals mixed in the dog and cat food; (5) The dog and cat food that has passed the metal detector is sampled and tested, and the low-temperature baked pet food composition for preventing and treating dog and cat diarrhea that meets the quality requirements is obtained after the test is qualified.

9. Use of the Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a pet product for preventing and treating diarrhea.

10. A pet product for preventing and treating pet diarrhea, characterized in that: The invention comprises the Chinese medicine composition according to any one of claims 1 to 4.

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