Efficient broiler feed and preparation method thereof

Abstract

The invention provides an efficient broiler feed. The efficient broiler feed is prepared from the following components in parts by mass: an energy component, a protein component, a mineral component, a purified poly-beta-carotene polymer, methionine and lysine, wherein the purified poly-beta-carotene polymer is obtained by purifying a poly-beta-carotene polymer through column chromatography; a chromatographic column of the column chromatography uses graphene oxide and diatomite composite adsorption microspheres and aluminum oxide as adsorption substances. According to the present invention, the poly-beta-carotene polymer is purified by using the chromatography column using the graphene oxide-diatomite composite adsorption microspheres and the alumina as the adsorption substances, such that the synergistic effect can be generated, the impurities of the poly-beta-carotene polymer can be effectively adsorbed so as to achieve the purpose of purifying the poly-beta-carotene polymer. The purified beta-carotene polymer is applied to efficient broiler feed and has the effects of promoting broiler growth, enhancing immunity and improving meat quality.

Description

Technical Field

[0001] The invention relates to the technical field of feed, and in particular to a high-efficiency broiler feed and a preparation method thereof. Background Art

[0002] In modern animal husbandry, broiler farming plays an important role. In view of consumers' high standards for chicken quality and yield, researchers continue to explore new methods and new substances to improve the growth efficiency and meat quality of broilers. With the advancement of biotechnology, many natural or synthetic substances have been included in the research scope of animal breeding.

[0003] Currently, a variety of strategies have been adopted to optimize the growth and meat quality of broilers, including the addition of vitamins, amino acids, minerals and other nutrients to feed, and the breeding of broiler breeds with fast growth and good meat quality through genetic selection. These measures have played a positive role in improving the production efficiency of broilers. Among many research fields, poly-β-carotene polymers are regarded as a substance with great potential due to their unique chemical properties and potential biological activity. They are expected to play an important role in promoting broiler growth, enhancing immunity and improving meat quality, and therefore have received extensive attention and research in the scientific research community.

[0004] Although poly-β-carotene polymers have a positive impact on broiler growth, immunity and meat quality, they still face challenges in practical application. One of the main problems is that the purification process of poly-β-carotene polymers is complicated, which not only affects the absorption efficiency of broilers, but also may not effectively improve meat quality due to the presence of impurities, and even pose a potential threat to broiler health, limiting the application potential of these substances in improving broiler growth performance.

[0005] In summary, it is necessary to develop a new technical solution to solve the problems existing in the prior art. Summary of the invention

[0006] The invention provides a high-efficiency broiler feed, wherein a purified poly-β-carotene polymer is added to the feed ingredients. The purified poly-β-carotene polymer is obtained by purifying the poly-β-carotene polymer by column chromatography; the chromatography column of the column chromatography uses graphene oxide diatomite composite adsorption microspheres and aluminum oxide as adsorption substances. The graphene oxide diatomite composite adsorption microspheres use graphene oxide with high dispersibility, diatomite with a porous structure, sodium alginate and calcium chloride as main raw materials, and utilize the expansion effect generated by the rapid evaporation of water molecules during microwave treatment, the template effect of diatomite, and the van der Waals force between graphene oxides and the nanofiber reinforcement effect to form composite adsorption microspheres with a three-dimensional porous structure. The adsorption microspheres have a pore structure, a large specific surface area, and a large number of active groups such as hydroxyl, carboxyl and cyclic amino groups, etc., and have strong adsorption performance. When used together with aluminum oxide which also has a porous structure and a high specific surface area, a synergistic effect can be produced to effectively adsorb impurities of the poly-β-carotene polymer, thereby achieving the purpose of purifying the poly-β-carotene polymer. The purified poly-β-carotene polymer is used in high-efficiency broiler feed, which has the effects of promoting broiler growth, enhancing immunity and improving meat quality.

[0007] One object of the present invention is to provide a high-efficiency broiler chicken feed, which is composed of the following components in parts by weight:

[0008]

[0009] in,

[0010] The purified poly-β-carotene polymer is obtained by purifying the poly-β-carotene polymer by column chromatography;

[0011] The chromatography column of the column chromatography method uses graphene oxide diatomaceous earth composite adsorption microspheres and aluminum oxide as adsorption substances.

[0012] Furthermore, the energy component is selected from one or more of corn, barley, sorghum, rice bran or wheat bran.

[0013] Furthermore, the protein component is selected from one or more of bean cake, peanut cake or fish meal.

[0014] Furthermore, the mineral component is selected from one or more of bone meal, shell meal or table salt.

[0015] Furthermore, the enzyme preparation is selected from one or more of xylanase, cellulase or amylase.

[0016] Another object of the present invention is to provide a method for preparing the above-mentioned high-efficiency broiler feed, comprising the following steps:

[0017] S1, dissolving sodium alginate in water to obtain a sodium alginate solution;

[0018] S2, adding graphene oxide to water, dispersing it by ultrasonic, then adding it to the sodium alginate solution, and then adding diatomaceous earth, stirring it by ultrasonic, to obtain a mixed solution;

[0019] S3, dissolving calcium chloride in water to obtain a calcium chloride solution;

[0020] S4, adding the mixed solution to the calcium chloride solution to react and obtain an intermediate product;

[0021] S5, treating the intermediate product with microwaves to obtain graphene oxide diatomite composite adsorption microspheres;

[0022] S6, dissolving the poly β-carotene polymer in acetone to obtain a poly β-carotene polymer solution;

[0023] S7, filling the graphene oxide diatomaceous earth composite adsorption microspheres and alumina as stationary phases into a chromatography column, adding the poly β-carotene polymer solution, and eluting with acetone as an eluent to obtain a purified poly β-carotene polymer;

[0024] S8. Evenly mix the energy component, the protein component, the mineral component, the purified poly-β-carotene polymer, methionine, lysine and the enzyme preparation to obtain a high-efficiency broiler feed.

[0025] Furthermore, in step S2, the mass ratio of graphene oxide, sodium alginate and diatomaceous earth is (0.01-0.1):1:(2-6).

[0026] Furthermore, in step S4, the mass ratio of the mixed solution to the calcium chloride solution solute is (1.5-3.5):1.

[0027] Furthermore, in step S6, the microwave power is 600-1200 W, and the processing time is 10-30 min.

[0028] Furthermore, in step S7, the mass ratio of the graphene oxide diatomaceous earth composite adsorption microspheres to alumina is 1:(2-3).

[0029] The present invention has the following beneficial effects:

[0030] The invention provides a high-efficiency broiler feed, wherein a purified poly-β-carotene polymer is added to the feed ingredients. The purified poly-β-carotene polymer is obtained by purifying the poly-β-carotene polymer by column chromatography; the chromatography column of the column chromatography uses graphene oxide diatomite composite adsorption microspheres and aluminum oxide as adsorption substances. The graphene oxide diatomite composite adsorption microspheres are composite adsorption microspheres with a three-dimensional porous structure formed by using graphene oxide with high dispersibility, diatomite with a porous structure, sodium alginate and calcium chloride as main raw materials, and utilizing the expansion effect generated by the rapid evaporation of water molecules during microwave treatment, the template effect of diatomite, and the van der Waals force between graphene oxides and the nanofiber reinforcement effect. The adsorption microspheres have a pore structure, a large specific surface area, and a large number of active groups such as hydroxyl, carboxyl and cyclic amino groups, etc., and have strong adsorption performance. When used together with aluminum oxide which also has a porous structure and a high specific surface area, a synergistic effect can be produced to effectively adsorb impurities of the poly-β-carotene polymer, thereby achieving the purpose of purifying the poly-β-carotene polymer. The purified poly-β-carotene polymer is used in high-efficiency broiler feed, which has the effects of promoting broiler growth, enhancing immunity and improving meat quality. DETAILED DESCRIPTION

[0031] In order to more clearly illustrate the technical solution of the present invention, the following examples are listed. Unless otherwise stated, the raw materials, reactions and post-treatment methods shown in the examples are common raw materials on the market and technical methods well known to those skilled in the art.

[0032] The words "preferred", "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects in certain circumstances. However, other embodiments may also be preferred under the same circumstances or other circumstances. In addition, the description of one or more preferred embodiments does not imply that other embodiments are not applicable, nor is it intended to exclude other embodiments from the scope of the present invention.

[0033] It should be understood that, except in any operating examples, or where otherwise indicated, all numbers indicating, for example, the amounts of ingredients used in the specification and claims should be understood to be modified in all cases by the term "about". Therefore, unless indicated to the contrary, the numerical parameters set forth in the following specification and the appended claims are approximate values ​​that vary depending on the desired properties to be obtained by the present invention.

[0034] The present invention uses the following raw materials:

[0035] Poly-β-carotene polymer (purity ≥ 95%, nor-isoprene compounds ≤ 5%) is produced in-house.

[0036] Example 1

[0037] A highly efficient broiler chicken feed, comprising the following components in parts by weight:

[0038]

[0039]

[0040] The method for preparing the above-mentioned high-efficiency broiler chicken feed comprises the following steps:

[0041] S1. Dissolve 2.5 g of sodium alginate in 100 mL of deionized water to obtain a sodium alginate solution;

[0042] S2, adding 0.15 g of graphene oxide to 50 mL of deionized water, ultrasonically dispersing for 2 h, then adding to the sodium alginate solution, and then adding 10 g of diatomaceous earth, ultrasonically stirring for 20 min to obtain a mixed solution;

[0043] S3, dissolving 5 g of calcium chloride in 100 mL of deionized water to obtain a calcium chloride solution;

[0044] S4, adding the mixed solution to the calcium chloride solution and reacting for 60 minutes to obtain an intermediate product;

[0045] S5, treating the intermediate product under a microwave power of 1000 W for 20 min to obtain graphene oxide diatomite composite adsorption microspheres;

[0046] S6, dissolving 25 g of poly β-carotene polymer in 50 mL of acetone to obtain a poly β-carotene polymer solution;

[0047] S7, filling the graphene oxide diatomite composite adsorption microspheres and alumina (graphene oxide diatomite composite adsorption microspheres: alumina = 2:5, m / m) as stationary phases into a chromatography column, adding the poly β-carotene polymer solution, eluting with acetone as an eluent, collecting the eluate, filtering, washing, and drying to obtain a yellow solid substance, i.e., purified poly β-carotene polymer;

[0048] S8. Evenly mix the energy component, protein component, mineral component, purified poly-β-carotene polymer, methionine, lysine and enzyme preparation according to the above mass fractions to obtain a high-efficiency broiler feed.

[0049] Example 2

[0050] A highly efficient broiler chicken feed, comprising the following components in parts by weight:

[0051]

[0052]

[0053] The preparation method of the above-mentioned high-efficiency broiler feed is the same as that in Example 1.

[0054] Example 3

[0055] A highly efficient broiler chicken feed, comprising the following components in parts by weight:

[0056]

[0057] The preparation method of the above-mentioned high-efficiency broiler feed is the same as that in Example 1.

[0058] Comparative Example 1

[0059] A high-efficiency broiler feed. The difference between this comparative example and Example 1 is that step S1-5 is omitted, and in step S7, the mass of the graphene oxide diatomaceous earth composite adsorption microspheres is replaced by diatomaceous earth, and the dosage of other components and the preparation method are the same as those in Example 1.

[0060] Comparative Example 2

[0061] A high-efficiency broiler feed. The difference between this comparative example and Example 1 is that step S1-7 is omitted, and in step S8, the purified poly-β-carotene polymer is replaced by poly-β-carotene polymer, and the dosage of other components and the preparation method are the same as those in Example 1.

[0062] Test Case

[0063] 320 30-day-old native chickens were taken, including 160 roosters weighing 142.5±1.7g, and 160 hens weighing 115.2±1.6g; the roosters and hens were randomly divided into 5 groups, with 20 hens and 20 roosters in each group; they were fed with the feed prepared in Implementation 1-3 and Comparative Example 1-2 respectively; they were allowed to eat and drink freely, and after 2 months, the growth of the native chickens was observed, and the results are shown in Table 1.

[0064]

[0065]

[0066] It can be seen from the above test results that when the high-efficiency broiler chicken feed of the present invention is used to feed native chickens, the average daily feed intake of the native chickens increases, the growth rate accelerates, the immunity is stronger, and the survival rate is higher.

[0067] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0068] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A highly efficient broiler chicken feed, characterized in that: The following components are included by weight: composition: in, The purified poly-β-carotene polymer is obtained by purifying the poly-β-carotene polymer by column chromatography; The chromatography column of the column chromatography method uses graphene oxide diatomaceous earth composite adsorption microspheres and aluminum oxide as adsorption substances.

2. The high-efficiency broiler feed according to claim 1, characterized in that: The energy component is selected from one or more of corn, barley, sorghum, rice bran or wheat bran.

3. The high-efficiency broiler feed according to claim 1, characterized in that: The protein component is selected from one or more of bean cake, peanut cake or fish meal.

4. The high-efficiency broiler feed according to claim 1, characterized in that: The mineral component is selected from one or more of bone meal, shell meal or table salt.

5. The high-efficiency broiler feed according to claim 1, characterized in that: The enzyme preparation is selected from one or more of xylanase, cellulase or amylase.

6. The method for preparing the high-efficiency broiler feed according to any one of claims 1 to 5, characterized in that: The steps include: S1, dissolving sodium alginate in water to obtain a sodium alginate solution; S2, adding graphene oxide to water, dispersing it by ultrasonic, then adding it to the sodium alginate solution, and then adding diatomaceous earth, stirring it by ultrasonic, to obtain a mixed solution; S3, dissolving calcium chloride in water to obtain a calcium chloride solution; S4, adding the mixed solution to the calcium chloride solution to react and obtain an intermediate product; S5, treating the intermediate product with microwaves to obtain graphene oxide diatomite composite adsorption microspheres; S6, dissolving the poly β-carotene polymer in acetone to obtain a poly β-carotene polymer solution; S7, filling the graphene oxide diatomaceous earth composite adsorption microspheres and alumina as stationary phases into a chromatography column, adding the poly β-carotene polymer solution, and eluting with acetone as an eluent to obtain a purified poly β-carotene polymer; S8. Evenly mix the energy component, the protein component, the mineral component, the purified poly-β-carotene polymer, methionine, lysine and the enzyme preparation to obtain a high-efficiency broiler feed.

7. The method for preparing the high-efficiency broiler chicken feed according to claim 6, characterized in that: In step S2, the mass ratio of graphene oxide, sodium alginate and diatomaceous earth is (0.01-0.1):1:(2-6).

8. The method for preparing the high-efficiency broiler feed according to claim 6, characterized in that: In step S4, the mass ratio of the mixed solution to the calcium chloride solution solute is (1.5-3.5):

1.

9. The method for preparing the high-efficiency broiler feed according to claim 6, characterized in that: In step S6, the microwave power is 600-1200 W, and the processing time is 10-30 min.

10. The method for preparing the high-efficiency broiler feed according to claim 6, characterized in that: In step S7, the mass ratio of the graphene oxide diatomite composite adsorption microspheres to alumina is 1:(2-3).

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