Device for separating renal membrane of mouse and transplanting ovarian tissue under renal membrane

Abstract

The device comprises a tissue separation sampling tube, an arc-shaped sampling opening is formed in the front end of the tissue separation sampling tube, a push rod capable of moving in a reciprocating mode is arranged in the tissue separation sampling tube, a sealing plug is arranged on the push rod, and the sealing plug is connected with the arc-shaped sampling opening. The sealing plug is tightly attached to the inner wall of the tissue separation sampling tube; the device has the beneficial effects that the arc-shaped sampling opening is formed in the front end of the tissue separation sampling tube, so that the device can be used for sampling and renal membrane separation at the same time, and tissue damage is greatly reduced; and through the reciprocating push rod, the operation process is simplified and optimized, and on the premise of protecting the tissue integrity, the success rate and the efficiency of mouse renal envelope separation and renal subenvelope ovarian tissue transplantation operation are improved, so that the development of related researches is powerfully promoted.

Description

Technical Field

[0001] The invention relates to the field of basic medical research technology, and in particular to a device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule. Background Art

[0002] The fertility problems of patients with premature ovarian insufficiency (POI) and premature ovarian failure (POF) have always been a difficult problem, a frontier and a hot topic in the field of international reproductive medicine. At present, whether it is auxiliary drugs, various ovulation induction schemes for in vitro fertilization and embryo transfer, in vitro activation of ovarian primordial follicles and stem cell therapy, the overall efficacy is still very low. Therefore, exploring safer and more efficient in vitro activation technology of ovarian primordial follicles and providing new ideas for the clinical diagnosis and treatment of POI and POF patients is an urgent problem to be solved in the current reproductive field.

[0003] In the field of reproductive biology and experimental animal research, in vitro activation of primordial follicles and ovarian tissue transplantation is an important research direction. In animal experimental studies, activated ovarian tissue is often transplanted under the renal capsule of the recipient mouse. This technology usually requires complex surgical operations and sophisticated separation techniques. During the operation, the recipient mouse kidneys must first be exposed and the renal capsule separated, and then the activated ovarian tissue is transplanted under the renal capsule of the recipient mouse. This process requires high skills from the experimenter. The separation process can easily lead to tissue damage, bleeding, and renal capsule rupture, making it impossible to successfully transplant the ovarian tissue, resulting in experimental failure or difficult operation, prolonged operation time, affecting the accuracy and reliability of the experimental results.

[0004] The patent with the publication number "CN115813512A" discloses "an ovarian tissue slice in vitro activated postoperative transplantation device". Although the transplantation of tissue slices is achieved, the transplantation process may cause damage to the tissue at the recipient's transplantation site; and it does not have a guide structure, which may affect the transplantation accuracy due to the long length of the device itself. At the same time, this patent is mainly used for the transplantation of human ovarian tissue, and the volume of each structure is large, so it cannot be used for mouse experimental research. Summary of the invention

[0005] The purpose of the present invention is to provide a device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule, so as to improve the efficiency and accuracy of separating mouse renal capsule and transplanting ovarian tissue under the renal capsule and reduce tissue damage.

[0006] To achieve the above object, the present invention adopts the following technical solutions: A device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule comprises a tissue separation sampling tube, the front end of which is provided with an arc-shaped sampling port, a reciprocating push rod is arranged in the tissue separation sampling tube, a sealing plug is arranged on the push rod, and the sealing plug is tightly fitted with the inner wall of the tissue separation sampling tube.

[0007] Preferably, the sealing plug is located at the front end of the push rod.

[0008] Preferably, the device further comprises a support tube for the push rod to pass through.

[0009] Preferably, a guide block is provided on the push rod, and the guide block abuts against the inner wall of the support tube.

[0010] Preferably, a guide rod is provided on the inner wall of the support tube, and a groove cooperating with the guide rod is provided on the guide block.

[0011] Preferably, the inner wall of the support tube is provided with a first limit block and a second limit block for limiting the moving distance of the guide block.

[0012] Preferably, the sealing plug is provided with a receiving groove which cooperates with the front end of the push rod.

[0013] Preferably, the accommodating groove passes through the sealing plug, the push rod passes through the sealing plug along the accommodating groove, and a fixing ring for fixing the sealing plug is provided on the push rod.

[0014] Preferably, a handle is provided at the tail of the push rod.

[0015] Preferably, the tissue separation sampling tube is provided with scale lines, and the handle is provided with an anti-slip portion.

[0016] The beneficial effects of the present invention are: 1. The device has an arc-shaped sampling port at the front end of the tissue separation sampling tube, which can be used for sampling and renal capsule separation at the same time, greatly reducing tissue damage; and through the reciprocating push rod, the operation process is simplified and optimized. Under the premise of protecting tissue integrity, the success rate and efficiency of mouse renal capsule separation and subrenal capsule ovarian tissue transplantation operations are improved, thereby effectively promoting the progress of related research.

[0017] 2. The device makes the movement of the push rod more stable and reliable through the setting of the support tube and the internal guide rod, reduces the operating difficulty of medical staff and improves the transplantation accuracy.

[0018] 3. By setting the first limit block and the second limit block, the maximum distance of movement of the guide block and the push rod is limited, which can effectively prevent them from escaping from the tissue separation sampling tube (when moving backward) and can also prevent them from being excessively ejected from the tissue separation sampling tube (when moving forward).

[0019] 4. A receiving groove is provided on the sealing plug, which makes the connection between it and the push rod more stable and reliable, greatly improving the performance of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a front view of the present invention; Figure 2 for Figure 1 A-A sectional view; Figure 3 for Figure 2 B-B sectional view; Figure 4 It is a schematic diagram of the second connection structure between the sealing plug and the push rod; Figure 5 Schematic diagram of the third connection structure between the sealing plug and the push rod.

[0021] The drawings are only used for illustrative purposes and should not be construed as limitations on this patent. In order to better illustrate this embodiment, some parts of the drawings may be omitted, enlarged, or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted. DETAILED DESCRIPTION

[0022] The present invention is further described below in conjunction with the accompanying drawings. Example

[0023] like Figure 1 and Figure 2 As shown, a mouse renal capsule separation and sub-renal capsule ovarian tissue transplantation device comprises a tissue separation sampling tube 1, the front end of the tissue separation sampling tube 1 has an arc-shaped sampling port 11, and the sampling port 11 is smooth and blunt, which can easily separate the renal capsule and reduce bleeding damage. A reciprocating push rod 2 is arranged in the tissue separation sampling tube 1, and a sealing plug 21 is arranged on the push rod 2, and the sealing plug 21 is located at the front end of the push rod 2. The sealing plug 21 is tightly fitted with the inner wall of the tissue separation sampling tube 1. When the push rod 2 moves backward, a simple vacuum state can be formed at the front end of the tissue separation sampling tube 1, so that the ovarian tissue can be smoothly adsorbed. When the push rod 2 moves forward, the adsorbed ovarian tissue can be discharged (it is expected that the air at the front end of the tissue separation sampling tube 1 can be squeezed out to better reduce tissue damage; however, in actual use, there is also a situation where the resistance of the tissue in the tube is too large, resulting in the tissue being unable to be smoothly squeezed out by the air, and at this time, it can be pushed out by the sealing plug 21), so that it can be smoothly transferred to the sub-renal capsule.

[0024] A handle 4 is provided at the rear of the push rod 2 to facilitate the forward and backward movement of the push rod 2 .

[0025] like Figure 1and Figure 2 As shown, the device also includes a support tube 3 located between the tissue separation sampling tube 1 and the handle 4, and the push rod 2 passes through the support tube 3. A guide block 22 is provided on the push rod 2, and the guide block 22 abuts against the inner wall of the support tube 3. The support tube 3 supports the push rod 2 to the left and right, so that it moves forward and backward more smoothly. In the specific use process, in order to ensure the adsorption effect on the ovarian tissue, the diameter of the tissue separation sampling tube 1 and the sampling port 11 is relatively thin, and in order to provide a certain operating space for the operator, the device itself needs to have a certain length, so the device is slender as a whole. In order to ensure that the push rod 2 does not deflect during the movement, a plurality of guide blocks 22 can be provided on the push rod 2.

[0026] like Figure 2 and Figure 3 As shown, the inner wall of the support tube 3 is provided with a guide rod 31, and the guide block 22 is provided with a slot that matches the guide rod 31. In this embodiment, the guide rods 31 are symmetrically arranged at two locations on the inner wall of the support tube 3. In other embodiments, any number of guide rods 31 can be arranged according to actual needs. The arrangement of the guide rods 31 and the slots can make the movement process of the push rod 2 more stable.

[0027] The inner wall of the support tube 3 is provided with a first limit block 32 and a second limit block 33 for limiting the moving distance of the guide block 22. In this embodiment, the first limit block 32 limits the forward movement of the guide block 22 to prevent the push rod 2 integrally provided with the guide block 22 from excessively moving forward (when in use, in order to discharge the ovarian tissue in the tissue separation sampling tube 1, it may be necessary to push the sealing plug 21 out of the tube, but it cannot be pushed out too much, because it will cause the sealing plug 21 to directly contact the ovarian tissue under force), resulting in damage to the ovarian tissue; the first limit block 32 limits the backward movement of the guide block 22 to prevent it from falling out of the tissue separation sampling tube 1 (or causing the ovarian tissue to penetrate too deeply into the tissue separation sampling tube 1).

[0028] like Figure 4 As shown, the sealing plug 21 is provided with a receiving groove which matches with the front end of the push rod 2. Figure 2 As shown, the mating surfaces of the sealing plug 21 and the push rod 2 are flat, and they are bonded together by glue. To improve the bonding effect, a receiving groove may be provided on the sealing plug 21 in this embodiment. After the front end of the push rod 2 is inserted into the receiving groove, the two are bonded together by glue, which can effectively prevent the sealing plug 21 from falling off.

[0029] like Figure 5As shown, the receiving groove can also penetrate the sealing plug 21, and the push rod 2 passes through the sealing plug 21 along the receiving groove, and a fixing ring 23 for fixing the sealing plug is provided on the push rod 2. The fixing ring 23 can use a nut, and a thread matching with it is provided at the front end of the push rod 2. First, a fixing ring 23 is provided on the push rod 2, and then the sealing plug 21 is provided on the push rod 2, and then another fixing ring 23 is provided, and then the fixing ring 23 is tightened to fix the sealing plug 21.

[0030] like Figure 1 As shown, for a better user experience, the device is further provided with a scale line 12 on the tissue separation sampling tube 1 to facilitate observation by the operator, and an anti-slip portion 41 is provided on the handle 4 .

[0031] During use of the device, the sampling port 11 of the tissue separation sampling tube 1 is first placed on the ovarian tissue to be sampled (the tissue is generally placed in a cell culture dish), and then the push rod 2 is pulled backward to adsorb the ovarian tissue into the tissue separation sampling tube 1, and then the sampling port 11 of the tissue separation sampling tube 1 is placed on the renal capsule of the recipient, and the renal capsule is first separated through the sampling port 11, and then the push rod 2 is pushed forward to transfer the ovarian tissue in the tissue separation sampling tube 1 to under the renal capsule of the recipient.

[0032] The device disclosed in this embodiment can realize the following two processes (separation of renal capsule and transplantation of tissue) in sequence, solving the problem of the prior art (requiring separation of renal capsule by a separation device first and then using a transplantation device for tissue transplantation), which requires more equipment, more complicated operation and higher skill level of the operator. The device can greatly reduce the operating pressure and improve the transplantation effect.

[0033] The above embodiments do not impose any formal limitations on the shape, material, structure, etc. of the present invention. Any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are within the protection scope of the technical solution of the present invention.

[0034] In the description of the present invention, it is necessary to understand that the terms "center", "longitudinal", "lateral", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the protection content of the present invention.

[0035] If the words "first", "second", etc. are used in this document to limit components, those skilled in the art should know that the use of "first" and "second" is only to facilitate the description of the present invention and simplify the description. Unless otherwise stated, the above words have no special meaning.

[0036] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule, comprising a tissue separation sampling tube, characterized in that: The front end of the tissue separation sampling tube has an arc-shaped sampling port. A reciprocating push rod is arranged in the tissue separation sampling tube. A sealing plug is arranged on the push rod. The sealing plug is tightly fitted with the inner wall of the tissue separation sampling tube.

2. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 1, characterized in that: The sealing plug is located at the front end of the push rod.

3. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 1, characterized in that: Also included is a support tube through which the push rod passes.

4. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 3, characterized in that: The push rod is provided with a guide block, and the guide block abuts against the inner wall of the support tube.

5. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 4, characterized in that: The inner wall of the support tube is provided with a guide rod, and the guide block is provided with a slot matched with the guide rod.

6. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 4, characterized in that: The inner wall of the support tube is provided with a first limit block and a second limit block for limiting the moving distance of the guide block.

7. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 2, characterized in that: The sealing plug is provided with a receiving groove matched with the front end of the push rod.

8. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 7, characterized in that: The accommodating groove passes through the sealing plug, the pushing rod passes through the sealing plug along the accommodating groove, and a fixing ring for fixing the sealing plug is arranged on the pushing rod.

9. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 1, characterized in that: A handle is arranged at the tail of the push rod.

10. The device for separating mouse renal capsule and transplanting ovarian tissue under the renal capsule according to claim 9, characterized in that: The tissue separation sampling tube is provided with scale lines, and the handle is provided with an anti-slip portion.

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