PDO barb suture line with antibacterial coating and preparation method of PDO barb suture line

Abstract

The invention relates to the technical field of medical sutures, and particularly discloses a PDO barb suture with an antibacterial coating and a preparation method of the PDO barb suture. According to the PDO barb suture line with the antibacterial coating, a PDO mixed suture line base body with good mechanical performance is obtained by blending, spinning and weaving L-polylactic acid and poly (p-dioxanone) according to a certain proportion; hyaluronic acid is used as a gel matrix, 4-(oxirane-2-yl methoxyl) butyl acrylate is grafted to hyaluronic acid for alkenyl modification, then the hyaluronic acid is mixed with quercetin-3-O-glucoside and TCS-ZIF-8 loaded with oridonin, and the antibacterial gel coating is obtained. And finally, dipping the PDO mixed suture line matrix into the antibacterial gel coating and carrying out ultraviolet irradiation in the presence of a photoinitiator to prepare the PDO mixed suture line. The suture line prepared by the invention has the advantages of smooth coating, efficient and lasting antibacterial performance, high strength, good flexibility, small friction force and high operability.

Description

Technical Field

[0001] The present application relates to the technical field of medical sutures, and more specifically, to a PDO barbed suture with an antibacterial coating and a preparation method thereof. Background Art

[0002] Degradable sutures have been widely studied due to their significant advantages of no need to remove sutures during wound healing and non-toxic and harmless degradation products. Polydioxanone (PDO) is often used to make degradable surgical sutures due to its excellent biodegradability, bioabsorbability, biocompatibility, flexibility and easy processing. However, traditional PDO sutures generally adopt a woven structure, and the concave and convex surface formed is easy to adhere to bacteria, causing surgical site infection, which will cause the wound to produce varying degrees of suppuration, inflammation and lesions. Therefore, it is necessary to treat the sutures with antibacterial properties.

[0003] However, the existing absorbable sutures in the prior art have various problems in clinical use. For example, Chinese patent CN204275113U uses polycaprolactone to carry the antibacterial drug levofloxacin hydrochloride to prepare an antibacterial coating solution, and coats the silk thread of the braided structure to obtain a medical suture with good antibacterial and sustained-release properties, but excessive use of antibiotics is harmful to the human body to a certain extent; Chinese patent CN105107016A discloses a highly antibacterial absorbable medical suture, which is made of carboxymethyl chitosan, polyglycolic acid, hyaluronic acid, silk protein, salmon collagen, glutaraldehyde, genipin, and glycosides. The prepared suture has good antibacterial properties, but has a short antibacterial time in the later stage. Chinese patent CN107213507A discloses an absorbable suture and a preparation method thereof. The suture is composed of polyglycolic acid (PGA), calcium stearate and chitosan and is prepared by a weaving process. Although it has good tensile strength and antibacterial properties, the weaved suture is relatively rough and easy to drag and damage tissues, and causes continuous friction and wear to the contact tissues after surgery.

[0004] Therefore, developing a suture with excellent mechanical properties and durable antibacterial properties can effectively prevent the risk of surgical site infection. Summary of the invention

[0005] In order to further improve the ability of sutures in preventing the risk of surgical site infection, the present application provides a PDO barbed suture with an antibacterial coating and a preparation method thereof.

[0006] In a first aspect, the present application provides a method for preparing a PDO barbed suture with an antibacterial coating, using the following technical solution:

[0007] A method for preparing a PDO barbed suture with an antibacterial coating comprises the following steps:

[0008] S1, preparing a PDO mixed suture matrix: mixing L-polylactic acid and polydioxanone in proportion, melting and kneading at 150-170° C. for 10-20 min, spinning and stretching, and weaving with a braiding machine to obtain a PDO mixed suture matrix;

[0009] S2, preparation of antibacterial gel coating:

[0010] S2.1, preparing alkenyl hyaluronic acid: dissolving hyaluronic acid uniformly in deionized water, then adding 4-(oxirane-2-ylmethoxy)butyl acrylate, stirring at 50-60° C. for 20-24 hours, then adding lysine solution at room temperature, stirring for 30-50 minutes to terminate the reaction, dialyzing in water for 3-4 days and then freeze-drying to obtain alkenyl hyaluronic acid;

[0011] S2.2, the alkenyl hyaluronic acid aqueous solution and the quercetin-3-O-glucoside methanol solution are mixed evenly, and then added to the TCS@ZIF-8 mixed solution, stirred for 3-5 hours, and a photoinitiator is added to obtain an antibacterial gel coating solution;

[0012] S3, immersing the PDO mixed suture matrix in the antibacterial gel coating solution for 2-3 hours, taking it out, vacuum drying it, and then irradiating it under ultraviolet light for 30-45 seconds. After cleaning and drying, the barbs are cut out to obtain the PDO barbed suture with antibacterial coating.

[0013] By adopting the above technical solution,

[0014] Preferably, the usage ratio of L-polylactic acid to polydioxanone in S1 is (35-55) g:(45-65) g.

[0015] By adopting the above technical scheme, a PDO mixed suture matrix with good mechanical properties is obtained by blending polylactic acid and polydioxanone in a certain proportion for spinning and weaving, and then hyaluronic acid is used as a gel matrix by grafting 4-(oxirane-2-ylmethoxy)butyl acrylate on the hyaluronic acid for olefin modification, and then mixed with quercetin-3-O-glucoside with antibacterial properties and TCS@ZIF-8 loaded with oridonin with antibacterial effect to obtain an antibacterial gel coating, and finally the PDO mixed suture matrix is ​​immersed in the antibacterial gel coating and irradiated with ultraviolet light under a photoinitiator to obtain a PDO barbed suture with an antibacterial coating having excellent mechanical properties and antibacterial durability.

[0016] Preferably, in S2.1, the dosage ratio of hyaluronic acid, deionized water, 4-(oxirane-2-ylmethoxy)butyl acrylate and lysine solution is (5-10) g:(500-800) mL:(15-20) mL:(20-27) mL; the concentration of the lysine solution is 20-25 wt%.

[0017] Hyaluronic acid, as a naturally occurring polysaccharide, is widely distributed in various tissues and body fluids of the human body. It has multiple active functional groups such as carboxyl, hydroxyl and amide. Through the ring-opening reaction of carboxyl and 4-(2-oxirane-1-ylmethoxy)butyl acrylate, a modified hydrogel with alkenyl functional groups is obtained, preparing for the subsequent loading of antibacterial components.

[0018] Preferably, in S2.2, the usage ratio of the alkenyl hyaluronic acid aqueous solution, the quercetin-3-O-glucoside methanol solution, the TCS@ZIF-8 solution, and the photoinitiator is (20-40) mL: (12-18) mL: (7-12) mL: (14-22) mg.

[0019] By adopting the above technical scheme, on the one hand, quercetin-3-O-glucoside has multiple functions such as antioxidant, anti-inflammatory, and antibacterial, and is used in wound suture, which is beneficial to wound healing and reduces postoperative infection. At the same time, quercetin-3-O-glucoside contains multiple hydrogen bonds and is UV-stable, which is beneficial to form hydrogen bonds with the carbonyl group in alkenyl hyaluronic acid and is not destroyed by ultraviolet light.

[0020] Preferably, in S2.2, the concentration of the alkenyl hyaluronic acid aqueous solution is 4.3-6.7wt%, the concentration of the quercetin-3-O-glucoside methanol solution is 3.5-5.8wt%, and the concentration of the TCS@ZIF-8 solution is 7.2-8.5wt%.

[0021] Preferably, the photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone.

[0022] By adopting the above technical scheme, the alkenyl undergoes polymerization reaction under external light to form a chemical cross-linked network. At the same time, the hydrogen bonding between the hydroxyl group of quercetin-3-O-glucoside and the carbonyl group in alkenyl hyaluronic acid is conducive to the formation of a physical cross-linked network. The double cross-linked network structure is conducive to the stable dispersion of TCS@ZIF-8.

[0023] Preferably, the preparation method of the TCS@ZIF-8 solution is as follows: (1) dissolving 2-methylimidazole and oridonin in methanol, then pouring the zinc nitrate solution into the mixture under stirring, stirring for 2-3 minutes, and then centrifuging at a speed of 6000-8000 r / min for 20-30 minutes, collecting the product, and washing it with methanol to obtain TCS@ZIF-8; (2) diluting TCS@ZIF-8 with methanol to obtain a 7.2-8.5wt% TCS@ZIF-8 mixed solution.

[0024] Preferably, the dosage ratio of the 2-methylimidazole, Rubescensine A, methanol, and zinc nitrate solution is (4.6-6.2) g: (80-100) mg: (120-180) mL: (75-92) mL; and the concentration of the nitric acid solution is 20-25 mg / mL.

[0025] Preferably, the stirring speed in (1) is 650-850 r / min.

[0026] By adopting the above technical scheme, by preparing nano-scale TCS@ZIF-8 with regular morphology and good biodegradability, Rubescensine A is loaded into the zeolite imidazolate framework, thereby avoiding the destruction and ineffectiveness of Rubescensine A during ultraviolet cross-linking, and at the same time enabling Rubescensine A to be slowly released, thereby enhancing the antibacterial persistence.

[0027] In a second aspect, the present application provides a PDO barbed suture with an antibacterial coating, which is obtained by the above-mentioned preparation method.

[0028] In summary, this application has the following beneficial effects:

[0029] 1. In the present application, L-polylactic acid and polydioxanone are blended and spun in a certain proportion to obtain a PDO mixed suture matrix with good mechanical properties, and then hyaluronic acid is used as a gel matrix by grafting 4-(oxirane-2-ylmethoxy)butyl acrylate on the hyaluronic acid for olefin modification, and then mixed with quercetin-3-O-glucoside with antibacterial properties and TCS@ZIF-8 loaded with antibacterial oridonin to obtain an antibacterial gel coating, and finally the PDO mixed suture matrix is ​​immersed in the antibacterial gel coating and irradiated with ultraviolet light under a photoinitiator to obtain a PDO barbed suture with an antibacterial coating having excellent mechanical properties and antibacterial durability.

[0030] 2. The present application preferentially uses 2-methylimidazole, zinc nitrate, and oridonin to prepare nano TCS@ZIF-8 particles with sustained release, biocompatibility, thermal stability, and water stability, and then adds them together with quercetin-3-O-glucoside to the olefinic gel coating; through photoinitiated olefinic polymerization, and coordinated hydrogen bonding between multiple hydroxyl groups in quercetin-3-O-glucoside and carbonyl groups in olefinic hyaluronic acid to form a chemical and physical double network structure, the TCS@ZIF-8 particles can be stably dispersed therein, and the woven gaps of the braided PDO mixed suture matrix are filled with the gel coating to form a smooth surface, thereby reducing tissue damage caused by the suture.

[0031] 3. The PDO barbed suture with antibacterial coating prepared by the preparation method of the present application has the characteristics of smooth coating, moderate thickness, high-efficiency and long-lasting antibacterial performance, high strength, good flexibility, low friction, and strong operability. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 : The reaction mechanism of the antibacterial gel coating of this application.

[0033] Figure 2 : Scanning electron microscope image of TCS@ZIF-8 prepared in Example 3 of the present application.

[0034] Figure 3 : Scanning electron micrograph of the PDO barbed suture with antibacterial coating prepared in Example 3 of the present application, wherein a is the PDO mixed suture matrix, b, c, d are the PDO barbed suture with antibacterial coating before the barbs are cut at different magnifications.

[0035] Figure 4 : Antibacterial test diagram of the PDO barbed suture with antibacterial coating prepared in Example 3 of the present application. DETAILED DESCRIPTION

[0036] The present application is further described in detail below with reference to the embodiments.

[0037] Unless otherwise specified, the raw materials used in the examples and comparative examples of the present application are all commercially available.

[0038] Example

[0039] Example 1

[0040] A method for preparing a PDO barbed suture with an antibacterial coating, characterized in that it comprises the following steps:

[0041] S1, preparing a PDO mixed suture matrix: 35 g of L-polylactic acid and 65 g of polydioxanone were fully dried and mixed, melted at 150° C. for 20 min, and stretched by spinning to obtain monofilaments, and then 8 monofilaments were used as shell yarns and 2 identical monofilaments were used as core yarns, and a domestic 8-spindle vertical medical braiding machine was used to prepare a type 0 braided suture, i.e., a PDO mixed suture matrix;

[0042] S2, preparation of antibacterial gel coating:

[0043] S2.1, preparing alkenyl hyaluronic acid: 5 g of hyaluronic acid was uniformly dissolved in 500 mL of deionized water, and then 15 mL of 4-(oxirane-2-ylmethoxy)butyl acrylate was added, and the mixture was stirred at 50° C. for 24 h, and then 20 mL of lysine solution with a concentration of 25 wt % was added at room temperature, and the reaction was terminated by stirring for 30 min. The mixture was dialyzed in water for 3 days and then freeze-dried to obtain alkenyl hyaluronic acid;

[0044] S2.2, 20 mL of 6.7 wt% alkenyl hyaluronic acid aqueous solution and 12 mL of 5.8 wt% quercetin-3-O-glucoside methanol solution were mixed evenly, then added into 7 mL of 8.5 wt% TCS@ZIF-8 mixed solution, stirred for 3 h, and 14 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone was added to obtain an antibacterial gel coating liquid;

[0045] S3, immersing the PDO mixed suture matrix in the antibacterial gel coating solution for 2 hours, taking it out, vacuum drying it, and then irradiating it under ultraviolet light for 30 seconds. After cleaning and drying, the barbs are cut out to obtain a PDO barbed suture with an antibacterial coating; the wavelength of the ultraviolet light is 450nm, and the light intensity is 7W / cm 2 .

[0046] The preparation method of the TCS@ZIF-8 solution is as follows: (1) 4.6 g of 2-methylimidazole and 80 mg of oridonin are dissolved in 120 mL of methanol, and then 75 mL of a 20 mg / mL zinc nitrate solution is poured into the mixture under stirring at 650 r / min, stirring is continued for 2 min, and then centrifuged at 6000 r / min for 30 min, the product is collected, and washed with methanol to obtain TCS@ZIF-8; (2) TCS@ZIF-8 is diluted with methanol to prepare a TCS@ZIF-8 mixed solution with a concentration of 8.5 wt%.

[0047] Example 2

[0048] A method for preparing a PDO barbed suture with an antibacterial coating, characterized in that it comprises the following steps:

[0049] S1, preparing a PDO mixed suture matrix: 45 g of L-polylactic acid and 55 g of polydioxanone were fully dried and mixed, melted at 160° C. for 15 min, and stretched by spinning to obtain monofilaments, and then 8 monofilaments were used as shell yarns and 2 identical monofilaments were used as core yarns, and a domestic 8-spindle vertical medical braiding machine was used to prepare a type 0 braided suture, i.e., a PDO mixed suture matrix;

[0050] S2, preparation of antibacterial gel coating:

[0051] S2.1, preparing alkenyl hyaluronic acid: 8 g of hyaluronic acid was uniformly dissolved in 650 mL of deionized water, and then 18 mL of 4-(oxirane-2-ylmethoxy)butyl acrylate was added, and the mixture was stirred at 55° C. for 22 h, and then 27 mL of lysine solution with a concentration of 20 wt % was added at room temperature, and the reaction was terminated by stirring for 40 min. The mixture was dialyzed in water for 3 days and then freeze-dried to obtain alkenyl hyaluronic acid;

[0052] S2.2, 40 mL of 4.3 wt% alkenyl hyaluronic acid aqueous solution and 18 mL of 3.5 wt% quercetin-3-O-glucoside methanol solution were mixed evenly, and then added to 12 mL of 7.2 wt% TCS@ZIF-8 mixed solution, stirred for 4 h, and 19 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone was added to obtain an antibacterial gel coating liquid;

[0053] S3, immersing the PDO mixed suture matrix in the antibacterial gel coating solution for 2.5 hours, taking it out, vacuum drying it, and then irradiating it under ultraviolet light for 40 seconds. After cleaning and drying, the barbs are cut out to obtain a PDO barbed suture with an antibacterial coating; the wavelength of the ultraviolet light is 450nm, and the light intensity is 7W / cm 2 .

[0054] The preparation method of the TCS@ZIF-8 solution is as follows: (1) 5.4 g of 2-methylimidazole and 90 mg of oridonin are dissolved in 150 mL of methanol, and then 83 mL of a zinc nitrate solution with a concentration of 22 mg / mL is poured into the solution under stirring at 750 r / min, stirring is continued for 3 min, and then centrifuged at a speed of 7000 r / min for 25 min, the product is collected, and washed with methanol to obtain TCS@ZIF-8; (2) TCS@ZIF-8 is diluted with methanol to prepare a TCS@ZIF-8 mixed solution with a concentration of 7.2 wt%.

[0055] Example 3

[0056] A method for preparing a PDO barbed suture with an antibacterial coating, characterized in that it comprises the following steps:

[0057] S1, preparing a PDO mixed suture matrix: 55 g of L-polylactic acid and 45 g of polydioxanone were fully dried and mixed, melted at 170°C for 10 min, and stretched by spinning to obtain monofilaments, and then 8 monofilaments were used as shell yarns and 2 identical monofilaments were used as core yarns, and a domestic 8-spindle vertical medical braiding machine was used to prepare a type 0 braided suture, i.e., a PDO mixed suture matrix;

[0058] S2, preparation of antibacterial gel coating:

[0059] S2.1, preparing alkenyl hyaluronic acid: 10 g of hyaluronic acid was uniformly dissolved in 800 mL of deionized water, and then 20 mL of 4-(oxirane-2-ylmethoxy)butyl acrylate was added, and the mixture was stirred at 60° C. for 20 h, and then 24 mL of lysine solution with a concentration of 23 wt % was added at room temperature, and the reaction was terminated by stirring for 50 min. The mixture was dialyzed in water for 4 days and then freeze-dried to obtain alkenyl hyaluronic acid;

[0060] S2.2, 30 mL of 5.4 wt% alkenyl hyaluronic acid aqueous solution and 15 mL of 4.2 wt% quercetin-3-O-glucoside methanol solution were mixed evenly, and then added into 10 mL of 7.8 wt% TCS@ZIF-8 mixed solution, stirred for 5 h, and 22 mg of photoinitiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone was added to obtain an antibacterial gel coating liquid;

[0061] S3, immersing the PDO mixed suture matrix in the antibacterial gel coating solution for 3 hours, taking it out, vacuum drying it, and then irradiating it under ultraviolet light for 45 seconds. After cleaning and drying, the barbs are cut out to obtain a PDO barbed suture with an antibacterial coating; the wavelength of the ultraviolet light is 450nm, and the light intensity is 7W / cm 2 .

[0062] The preparation method of the TCS@ZIF-8 solution is as follows: (1) 6.2 g of 2-methylimidazole and 100 mg of oridonin are dissolved in 180 mL of methanol, and then 92 mL of a zinc nitrate solution with a concentration of 25 mg / mL is poured into the solution under stirring at 850 r / min, stirring is continued for 3 min, and then centrifuged at a speed of 8000 r / min for 30 min, the product is collected, and washed with methanol to obtain TCS@ZIF-8; (2) TCS@ZIF-8 is diluted with methanol to prepare a TCS@ZIF-8 mixed solution with a concentration of 7.8 wt%.

[0063] The microstructure of the prepared TCS@ZIF-8 was observed using a scanning electron microscope. Figure 2 As shown. Figure 2It can be seen that TCS@ZIF-8 has a regular crystal structure and a uniform particle size of about 160 nm.

[0064] Comparative Example 1

[0065] The same as Example 3, except that the addition amount of 55 g of L-polylactic acid and 45 g of polydioxanone in S1 was adjusted to 60 g of L-polylactic acid and 40 g of polydioxanone.

[0066] Comparative Example 2

[0067] The same as Example 3, except that hyaluronic acid is directly used to replace alkenyl hyaluronic acid in S2.2.

[0068] Comparative Example 3

[0069] The same as Example 3, except that in S2.2, an equimolar content of Rubescensine A is used to replace TCS@ZIF-8.

[0070] Comparative Example 4

[0071] Same as Example 3, except that the concentration of the TCS@ZIF-8 mixed solution in S2.2 was adjusted to 10.0 wt%.

[0072] The same as Example 3, except that the quercetin-3-O-glucoside methanol solution was not added to S2.2.

[0073] Performance testing

[0074] Morphology observation: The PDO mixed suture matrix prepared in Example 3 and the PDO barbed suture with antibacterial coating before cutting the barbs were observed by scanning electron microscopy. Figure 3 As shown. Figure 3 As can be seen in (a), the PDO hybrid suture matrix has a regular structure and regular woven gaps on the surface. As can be seen in (b), the PDO barbed suture with antibacterial coating before cutting the barbs shows that the antibacterial gel coating is evenly covered on the PDO hybrid suture matrix and fills the woven gaps of the PDO hybrid suture matrix. The surface is smooth and the thickness of the antibacterial gel coating is about 0.04 mm. As can be seen in (c), the antibacterial gel coating is porous and evenly distributed. As can be seen in (d), the pore size is about 3.5 μm. The large number of surface pores in the antibacterial gel coating indicates that it has a strong water storage capacity. The amount of water can promote the transfer of water vapor and oxygen, which is beneficial to the adhesion and growth of cells, promotes cell metabolism, and accelerates the repair of suture wounds.

[0075] Mechanical properties test: The PDO barbed sutures with antibacterial coatings prepared in Examples 1 to 3 and Comparative Examples 1 to 5 and the PDO mixed suture matrix prepared in Example 3 were sampled, and their breaking strength and breaking elongation were tested on a yarn strength tester with a tensile rate set to 100 mm / min; the bending stiffness was tested by the cantilever beam method; and the dynamic friction was tested by a suture friction performance tester.

[0076] Antibacterial persistence test: The antibacterial properties of the PDO barbed sutures with antibacterial coatings prepared in Examples 1 to 3 and Comparative Examples 1 to 5 and the PDO mixed suture matrix prepared in Example 3 against Staphylococcus aureus and Escherichia coli were tested by agar plate diffusion method: the sutures were moved to a new culture dish every 24 hours until the antibacterial band disappeared and bacteria multiplied under the sutures. The time at this time was recorded and one day was subtracted to indicate the antibacterial time.

[0077] The results are shown in Table 1 and Figure 4 As shown;

[0078] Table 1 Performance test results

[0079]

[0080] As can be seen from Table 1, the PDO barbed sutures with antibacterial coatings prepared in Examples 1 to 3 of the present application have excellent mechanical properties and antibacterial durability. Combining the PDO mixed suture matrix prepared in Comparative Example 1 and Implementation 3, it can be seen that the suture prepared by the addition ratio of L-polylactic acid and polydioxanone described in the present application has both strength and flexibility; Combining Comparative Example 2, Comparative Example 3, and Comparative Example 5, it can be seen that the addition of olefin-functionalized hyaluronic acid, TCS@ZIF-8 loaded with oridonin, and quercetin-3-O-glucoside are all beneficial to improving the antibacterial durability of the PDO barbed suture with antibacterial coating, and has little effect on its mechanical properties. Combining the PDO mixed suture matrix prepared in Implementation 3, it can be seen that the PDO barbed suture with antibacterial coating prepared in the present application has a small dynamic friction, which means that when it is suturing the wound, the dragging damage to the contact tissue is small; Combining Comparative Example 4, it can be seen that when the concentration of the TCS@ZIF-8 mixed solution is too large, the dynamic friction of the PDO barbed suture with antibacterial coating will increase.

[0081] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, a person skilled in the art may make non-creative modifications to the present embodiment as needed, and such modifications shall be protected by the patent law as long as they are within the scope of protection required by the present application.

Claims

1. A method for preparing a PDO barbed suture with an antibacterial coating, characterized in that: The following steps are involved: S1, preparing a PDO mixed suture matrix: mixing L-polylactic acid and polydioxanone in proportion, melting and kneading at 150-170° C. for 10-20 min, spinning and stretching, and weaving with a braiding machine to obtain a PDO mixed suture matrix; S2, preparation of antibacterial gel coating: S2.1, preparing alkenyl hyaluronic acid: dissolving hyaluronic acid uniformly in deionized water, then adding 4-(oxirane-2-ylmethoxy)butyl acrylate, stirring at 50-60° C. for 20-24 hours, then adding lysine solution at room temperature, stirring for 30-50 minutes to terminate the reaction, dialyzing in water for 3-4 days and then freeze-drying to obtain alkenyl hyaluronic acid; S2.2, the alkenyl hyaluronic acid aqueous solution and the quercetin-3-O-glucoside methanol solution are mixed evenly, and then added to the TCS@ZIF-8 mixed solution, stirred for 3-5 hours, and a photoinitiator is added to obtain an antibacterial gel coating solution; S3, immersing the PDO mixed suture matrix in the antibacterial gel coating solution for 2-3 hours, taking it out, vacuum drying it, and then irradiating it under ultraviolet light for 30-45 seconds. After cleaning and drying, the barbs are cut out to obtain the PDO barbed suture with antibacterial coating.

2. The method for preparing the PDO barbed suture with antibacterial coating according to claim 1, characterized in that: The usage ratio of L-polylactic acid and polydioxanone in S1 is (35-55) g:(45-65) g.

3. The method for preparing the PDO barbed suture with antibacterial coating according to claim 2, characterized in that: In S2.1, the dosage ratio of hyaluronic acid, deionized water, 4-(oxirane-2-ylmethoxy)butyl acrylate and lysine solution is (5-10) g:(500-800) mL:(15-20) mL:(20-27) mL; the concentration of the lysine solution is 20-25 wt%.

4. The method for preparing the PDO barbed suture with antibacterial coating according to claim 3, characterized in that: In S2.2, the usage ratio of alkenyl hyaluronic acid aqueous solution, quercetin-3-O-glucoside methanol solution, TCS@ZIF-8 solution, and photoinitiator is (20-40) mL: (12-18) mL: (7-12) mL: (14-22) mg.

5. The method for preparing the PDO barbed suture with antibacterial coating according to claim 4, characterized in that: In the S2.2, the concentration of the alkenyl hyaluronic acid aqueous solution is 4.3-6.7wt%, the concentration of the quercetin-3-O-glucoside methanol solution is 3.5-5.8wt%, and the concentration of the TCS@ZIF-8 solution is 7.2-8.5wt%.

6. The method for preparing the PDO barbed suture with antibacterial coating according to claim 5, characterized in that: The photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone.

7. The method for preparing the PDO barbed suture with antibacterial coating according to claim 6, characterized in that: The preparation method of the TCS@ZIF-8 solution is as follows: (1) dissolving 2-methylimidazole and Rubescensine A in methanol, then pouring the zinc nitrate solution into the solution under stirring, stirring for 2-3 minutes, and then centrifuging at a speed of 6000-8000 r / min for 20-30 minutes, collecting the product, and washing it with methanol to obtain TCS@ZIF-8; (2) diluting TCS@ZIF-8 with methanol to obtain a 7.2-8.5wt% TCS@ZIF-8 mixed solution.

8. The method for preparing the PDO barbed suture with antibacterial coating according to claim 7, characterized in that: The dosage ratio of the 2-methylimidazole, Rubescensine A, methanol and zinc nitrate solution is (4.6-6.2) g: (80-100) mg: (120-180) mL: (75-92) mL; the concentration of the nitric acid solution is 20-25 mg / mL.

9. The method for preparing the PDO barbed suture with antibacterial coating according to claim 8, characterized in that: The stirring speed in (1) is 650-850 r / min.

10. A PDO barbed suture with an antibacterial coating, characterized in that: The method for preparing a PDO barbed suture with an antibacterial coating according to any one of claims 1 to 9 is obtained.

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