Sterilizing and filtering device and method for preparing sterile culture medium

Abstract

The invention relates to the technical field of culture medium preparation, in particular to a degerming and filtering device and method for preparing a sterile culture medium. Comprising a shell, the shell is fixedly connected with an input pipe, the input pipe is fixedly connected and communicated with a first control valve, the first control valve is fixedly connected and communicated with two upper connecting pipes, the upper connecting pipes are fixedly connected and communicated with a filter cartridge, the filter cartridge is fixedly connected and communicated with lower connecting pipes, and the two lower connecting pipes are jointly fixedly connected and communicated with a second control valve. The second control valve is fixedly connected and communicated with an output pipe; the two filter screens are installed on the adjacent upper connecting pipes correspondingly, and the upper connecting pipes are rotationally connected with flow pushing pieces; and the driving blade fan is rotationally connected to the input pipe. The flow pushing piece drives the surrounding solution to rotate at a low speed, bacteria accumulated in the filter screen move to the position not making contact with the filter screen, then the situation that the filter screen is blocked by the bacteria is relieved, and the single-time use time of the filter screen is prolonged.

Description

Technical Field

[0001] The invention relates to the technical field of culture medium preparation, and in particular to a sterilization filtration device and method for preparing sterile culture medium. Background Art

[0002] After the existing culture medium solution is prepared, it is necessary to use a 0.45μm+0.22μm composite sterilization filter element for sterilization filtration to screen out bacteria in the culture medium and prepare a sterile culture medium solution. The method of sterilization by the composite sterilization filter element can ensure that the culture medium is free from bacterial contamination while minimizing the nutrient loss of the culture medium (for example, some culture medium solutions contain vitamin components, which will decompose at high temperatures, so this part of the culture medium solution is not suitable for high-temperature disinfection and other methods. This part of the culture medium solution is usually sterilized and filtered using a composite sterilization filter element). However, since the filter diameter of the composite sterilization filter element used for sterilization filtration is too small, when the solution passes through the composite sterilization filter element, the bacteria to be filtered out accumulate in large quantities in the composite sterilization filter element, resulting in a problem that the filtration speed of the solution gradually decreases and the composite sterilization filter element is easy to clog, resulting in low filtration efficiency. Summary of the invention

[0003] In order to overcome the shortcomings of the existing composite sterilizing filter element, that is, the filtration efficiency is gradually reduced and the composite sterilizing filter element is prone to clogging when sterilizing and filtering the culture medium solution, the present invention provides a sterilizing filtration device and method for preparing a sterile culture medium.

[0004] The technical solution of the present invention is: a sterilizing filtration device for preparing sterile culture medium, comprising: a shell, the shell is fixedly connected with an input pipe, the input pipe is fixedly connected and connected with a first control valve, the first control valve is fixedly connected and connected with two upper pipes, the upper pipe is fixedly connected and connected with a filter cartridge, the filter cartridge is fixedly connected and connected with a lower pipe, the two lower pipes are commonly fixedly connected and connected with a second control valve, and the second control valve is fixedly connected and connected with an output pipe; a filter screen, having two, and corresponding to the two upper pipes one by one, respectively installed on adjacent upper pipes, and located in adjacent filter cartridges, the upper pipe is rotatably connected with a flow-pushing member, the flow-pushing member is used to push the liquid and bacteria at the inner wall of the filter screen to rotate at a low speed; a driving blade fan, rotatably connected to the input pipe, and a speed measuring module for detecting its own rotation speed is installed on it, and the two flow-pushing members and the driving blade fan are driven by a pulley belt; a collecting mechanism is arranged in the shell and used to collect bacteria in the filter screen.

[0005] Furthermore, the flow-pushing member is composed of a cylinder and circumferentially distributed vertical plates.

[0006] Furthermore, the collecting mechanism includes: two collecting shells, which correspond one-to-one to the two filter cartridges and are respectively installed on adjacent filter cartridges, the collecting shell is fixedly connected to the filter screen, and the collecting shell is fixedly connected to the collecting filter screen; two backflushing components, which correspond one-to-one to the two filter cartridges and are both arranged in the outer shell, and are used to backflush the filter screen when the adjacent filter screen is blocked.

[0007] Furthermore, the recoil component includes: a liquid storage barrel, which is fixed in the outer shell; a first piston rod, which is slidably connected to the adjacent liquid storage barrel, and the first piston rod divides the liquid storage barrel into an upper cavity and a lower cavity, the upper cavity of the liquid storage barrel is connected to the adjacent collection shell, and the lower cavity of the liquid storage barrel is connected to the adjacent lower connecting pipe; an interception component, which is arranged on the adjacent liquid storage barrel, and is used to intercept and collect bacteria entering the adjacent liquid storage barrel.

[0008] Furthermore, the interception assembly includes: a filter shell, rotatably connected to the adjacent liquid storage barrel; a movable member, slidably connected to the adjacent first piston rod, and the movable member is used to drive the adjacent filter shell to rotate; a first hydraulic telescopic rod, fixedly connected to the adjacent liquid storage barrel, and the telescopic end of the first hydraulic telescopic rod is fixedly connected to the adjacent moving member.

[0009] Furthermore, it also includes: a driving component, which is arranged in the outer shell and is used to drive the two first hydraulic telescopic rods to work in sequence, and the driving component includes: a driving cylinder, which is fixed in the outer shell; a driving member, which is slidably connected in the outer shell, and the driving member divides the driving cylinder into a first cavity and a second cavity, the first cavity in the driving cylinder is connected to one of the upper pipes, and the second cavity in the driving cylinder is connected to the other upper pipe; a fixed frame, which is fixed to the two liquid storage barrels, and a transmission gear is installed on the fixed frame, and the two first piston rods are meshed with the transmission gear on the fixed frame through a rack, and the driving member is fixed to one of the first piston rods.

[0010] Furthermore, it also includes: a pressure regulating component, which is arranged on the fixed frame, and is used to reduce the pressure in the upper connecting pipe that needs to recoil, and the pressure regulating component includes: a negative pressure cylinder, which includes two, both of which are fixed to the fixed frame, and a second piston rod is slidably connected in the negative pressure cylinder, and the negative pressure cylinder is connected to the adjacent upper connecting pipe. The valve stem of the first control valve is fixed with two fixing parts, and the fixing part is slidably connected to the adjacent second piston rod.

[0011] Furthermore, the volume of the negative pressure cylinder is greater than the volume of the driving cylinder.

[0012] Furthermore, it also includes: a second hydraulic telescopic rod, having two, which are respectively fixed to the adjacent negative pressure cylinders, a spring is installed between the telescopic end of the second hydraulic telescopic rod and its fixed part, the second piston rod is used to squeeze the telescopic end of the adjacent second hydraulic telescopic rod, and the first hydraulic telescopic rod is connected to the adjacent second hydraulic telescopic rod.

[0013] A sterilizing filtration method for preparing a sterile culture medium is applied to the sterilizing filtration device for preparing a sterile culture medium, and the specific method is as follows: S1: Control the first control valve and the second control valve to switch working states so that the two are connected to the same filter cartridge; S2: The solution is introduced into the input pipe. After passing through the filter screen in the filter cartridge in use, the solution is discharged outward. The solution is simultaneously filled into the adjacent liquid storage barrel and driving cylinder. The flow-pushing element drives the surrounding solution and bacteria to rotate, and the collection filter screen collects the bacteria in the adjacent filter screen. S3: The driving member moves under the pressure difference of the filter cartridges on both sides, so that the first piston rod in the working state moves to the upper side, and the other first piston rod moves to the lower side. At the same time, the valve stem drives the two fixing members to rotate, so that the second piston rod in the working state moves to the lower side, and the other second piston rod moves to the upper side. S4: When the filter screen in the working state is clogged, the first control valve and the second control valve are controlled to switch the working state, so that the solution enters another filter cartridge for filtration; S5: After the state of the first control valve is switched, the two second piston rods move toward each other, and the two filter shells rotate under the drive of the adjacent second piston rods. The two first piston rods move toward each other to backflush the clogged filter screen, and the filter shell on the backflush side intercepts the bacteria that fall off the clogged filter screen. S6: After the solution is filtered, clean the filter cartridge, liquid storage tank and filter shell.

[0014] The beneficial effect of the present invention compared with the prior art is that the present invention drives the surrounding solution to rotate at a low speed through the flow-pushing member, so that the bacteria accumulated in the filter are moved to a position that does not contact the filter, thereby reducing the clogging of the filter caused by bacteria and extending the single use time of the filter.

[0015] The present invention stores part of the solution in a liquid storage barrel in advance. When the filter screen is seriously clogged, the flow path of the solution is switched. Under the premise of not affecting the filtration of the solution, the filter screen is backwashed with the solution in the liquid storage barrel, thereby cleaning the filter screen and restoring the use function of the filter screen.

[0016] The present invention generates negative pressure through a negative pressure cylinder and utilizes the pressure difference in two filter cylinders to automatically drive a solution to clean a clogged filter screen and collect bacteria cleaned from the filter screen, thereby increasing the degree of automation of the device, saving the energy and time spent on manual cleaning and increasing the scope of application of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 It is a three-dimensional structural schematic diagram of the first control valve, the upper connecting pipe and the filter cartridge of the present invention; Figure 3 It is a cross-sectional view of the filter cartridge and the liquid storage barrel of the present invention; Figure 4 It is a three-dimensional structural schematic diagram of the filter screen, bottom plate and flow-pushing member of the present invention; Figure 5 It is a cross-sectional view of the input pipe, upper pipe and filter cartridge of the present invention; Figure 6 It is a cross-sectional view of the filter cartridge, the collection shell and the liquid storage barrel of the present invention; Figure 7 It is a schematic diagram of the three-dimensional structure of the liquid storage barrel, the first piston rod and the filter housing of the present invention; Figure 8 It is a schematic diagram of the three-dimensional structure of the first hydraulic telescopic rod and the second hydraulic telescopic rod of the present invention; Fig. 9 It is a three-dimensional structural schematic diagram of the filter housing, the driving cylinder and the driving member of the present invention; Fig.10 It is a schematic diagram of the three-dimensional structure of the first control valve, the upper connecting pipe and the negative pressure cylinder of the present invention.

[0018] The names and serial numbers of the parts in the figure are: 1-housing, 101-input pipe, 102-first control valve, 1021-valve stem, 103-upper connecting pipe, 104-filter cartridge, 105-lower connecting pipe, 106-second control valve, 107-output pipe, 2-filter screen, 201-bottom plate, 202-flow-pushing part, 203-driving impeller, 3-collecting shell, 301-collecting filter screen, 302-liquid storage barrel, 303-first piston rod, 304-filter shell, 305-moving part, 306-first hydraulic telescopic rod, 4-driving cylinder, 401-driving part, 5-fixed frame, 501-negative pressure cylinder, 502-second piston rod, 503-fixed part, 504-second hydraulic telescopic rod. DETAILED DESCRIPTION

[0019] In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, or a detachable connection, or a connection assisted by parts. The term "connection" should be understood in a broad sense, for example, it can be direct connection, or it can be connected through a hose or a connecting pipe. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0020] Example 1: A sterile filtration device for preparing sterile culture medium, referring to Figure 1-Figure 5 , including: a housing 1, the housing 1 is fixedly connected to an input pipe 101, the input pipe 101 is fixedly connected to and communicated with a first control valve 102, the first control valve 102 is fixedly connected to and communicated with two upper pipes 103, the upper pipe 103 is fixedly connected to and communicated with a filter cartridge 104, the filter cartridge 104 is fixedly connected to and communicated with a lower pipe 105, the two lower pipes 105 are commonly fixedly connected to and communicated with a second control valve 106, the second control valve 106 is fixedly connected to and communicated with an output pipe 107; a filter screen 2, having two, and being connected to the two upper pipes 103 One by one, they are respectively installed on adjacent upper connecting pipes 103 and located in adjacent filter cartridges 104. The upper connecting pipe 103 is rotatably connected to a flow-pushing member 202, which is used to push the liquid and bacteria on the inner wall of the filter screen 2 to rotate at a low speed; the driving blade fan 203 is rotatably connected to the input pipe 101, and a speed measuring module for detecting its own rotation speed is installed thereon. The two flow-pushing members 202 and the driving blade fans 203 are driven by pulley belts; a collecting mechanism is arranged in the outer casing 1, and is used to collect bacteria in the filter screen 2.

[0021] Further, see Figure 5 The flow-pushing member 202 is composed of a cylinder and circumferentially distributed vertical plates.

[0022] In this embodiment, the purpose is to address the problem that the filtration efficiency of the existing composite sterilizing filter element is gradually reduced and the composite sterilizing filter element is prone to clogging when sterilizing and filtering the culture medium solution. The first control valve 102 and the second control valve 106 can be electrically controlled valves or hydraulically controlled valves. In this embodiment, the first control valve 102 and the second control valve 106 are electrically controlled valves, and the first control valve 102 and the second control valve 106 are electrically controlled valves. The first control valve 102 and the second control valve 106 are electrically connected to the control terminal. The first control valve 102 and the second control valve 106 are only connected to the same filter cartridge 104 at the same time. In this embodiment, in the initial state, the first control valve 102 and the second control valve 106 are both connected to the filter cartridge 104 on the left; the cylinder body of the filter cartridge 104 is set to an easy-to-disassemble structure, which is convenient for the staff to disassemble the filter cartridge 104 when needed. And replace the inner filter 2; the filter 2 is composed of a composite filter of a cylindrical structure and a bottom plate 201 at the bottom of the filter; the speed measuring module for detecting its own rotation speed on the driving blade 203 is an existing electronic control module, and the speed measuring module is electrically connected to the control terminal. The control terminal determines the flow rate of the solution in the input pipe 101 by detecting the rotation speed of the driving blade 203, and then determines whether the filter 2 in the current use state is seriously blocked. The flow-pushing member 202 is composed of a cylinder and circumferentially distributed vertical plates. When the flow-pushing member 202 rotates, it only drives the surrounding solution to rotate circumferentially, and will not guide the surrounding solution in the vertical direction, thereby promoting the solution to pass through the filter 2 evenly for filtration. The flow-pushing member 202 drives the bacteria to flow slowly circumferentially by pushing the surrounding solution, thereby reducing the probability of bacteria accumulating on the inner side of the filter 2.

[0023] The working process is as follows: when the staff is sterilizing and filtering the solution, the solution is passed into the input pipe 101. Because the first control valve 102 and the second control valve 106 are both connected to the filter cartridge 104 on the left in the initial state, the solution flows into the filter cartridge 104 on the left along the input pipe 101, the first control valve 102 and the upper connecting pipe 103 on the left. After the solution enters the filter cartridge 104 on the left, it passes through the left filter screen 2 and flows to the lower connecting pipe 105 on the left. The solution flows into the existing sterile collection barrel through the path of the lower connecting pipe 105 on the left, the second control valve 106 to the output pipe 107.

[0024] When the solution flows through the input pipe 101, the solution flows and drives the driving blade fan 203 to rotate. The control terminal monitors the rotation speed of the driving blade fan 203 through the speed measuring module on the driving blade fan 203. During the rotation of the driving blade fan 203, the two flow-pushing parts 202 are driven to rotate together through the pulley belt. The right-side flow-pushing part 202 is idling, and the left-side flow-pushing part 202 drives the surrounding solution to rotate slowly when rotating. When the solution around the left-side flow-pushing part 202 rotates, it continuously flushes the adjacent filter screen 2 along the spiral path, so that the bacteria intercepted on some of the filter screens 2 move along the inner surface of the filter screen 2. The collecting mechanism takes the opportunity to collect the bacteria moving along the inner surface of the left filter screen 2, thereby reducing the content of bacteria in the left filter screen 2 and the probability of bacteria getting stuck in the mesh of the filter screen 2, thereby facilitating the solution to pass through the left filter screen 2 and be discharged outward.

[0025] As the use time of the left filter 2 gradually increases, the number of bacteria stuck in the mesh of the left filter 2 gradually increases, and the left filter 2 is blocked on a large scale. At this time, the speed of the solution passing through the left filter 2 slows down, the flow rate of the solution at the input pipe 101 slows down, and the rotation speed of the driving blade fan 203 slows down synchronously. Because the speed measurement module on the driving blade fan 203 feeds back the rotation speed of the driving blade fan 203 to the control terminal in real time, the control terminal slows down the rotation speed of the driving blade fan 203 to a certain value (the value of the rotation speed can be adjusted by the staff according to different working conditions and requirements). Adjustment), the control terminal controls the first control valve 102 and the second control valve 106 to work simultaneously, the first control valve 102 and the second control valve 106 are disconnected from the left filter cartridge 104 at the same time, and are connected to the right filter cartridge 104, at this time the filter screen 2 on the right filters the solution through the same principle as mentioned above, while the collection mechanism on the left cleans and collects the intercepted bacteria in the left filter screen 2, so that when the right filter screen 2 is blocked on a large scale, the first control valve 102 and the second control valve 106 are controlled to switch to the initial state again to continue to sterilize and filter the solution.

[0026] When the staff has filtered all the solutions, the staff will clean the device and completely remove the bacteria collected by the collection mechanism.

[0027] Further, see Figure 3-Figure 6 The collecting mechanism includes: two collecting shells 3, which correspond to the two filter cartridges 104 one by one and are respectively installed on adjacent filter cartridges 104; the collecting shell 3 is fixedly connected to the filter screen 2; the collecting shell 3 is fixedly connected to the collecting filter screen 301; two backflushing components, which correspond to the two filter cartridges 104 one by one and are both arranged in the outer shell 1, for backflushing the filter screen 2 when the adjacent filter screen 2 is blocked.

[0028] Further, see Figure 2 , Figure 3 and Figure 5-Figure 7The recoil component includes: a liquid storage barrel 302, which is fixed to the outer shell 1; a first piston rod 303, which is slidably connected to the adjacent liquid storage barrel 302, and the first piston rod 303 divides the liquid storage barrel 302 into an upper cavity and a lower cavity, the upper cavity of the liquid storage barrel 302 is connected to the adjacent collecting shell 3, and the lower cavity of the liquid storage barrel 302 is connected to the adjacent lower connecting pipe 105; an interception component is arranged on the adjacent liquid storage barrel 302, and is used to intercept and collect bacteria entering the adjacent liquid storage barrel 302.

[0029] In this embodiment, the surface of the collecting shell 3 close to the axis side of the adjacent filter screen 2 is connected to the inner surface of the adjacent filter screen 2, so that the bacteria can flow along the inner surface of the filter screen 2 to the adjacent collecting shell 3. The collecting filter screen 301 is inclined (the inclination direction is as shown in FIG. Figure 5 As shown in the figure, the flow-pushing member 202 rotates clockwise (from top to bottom) under the drive of the driving blade 203, and the flow-pushing member 202 cooperates with the collecting filter 301 to prevent the bacteria in the collecting shell 3 from flowing to the side away from the collecting shell 3 in the process of following the flow of the solution; the volume of the liquid storage barrel 302 is greater than the volume of the adjacent filter cartridge 104; because the upper cavity of the liquid storage barrel 302 is connected to the adjacent collecting shell 3, and the lower cavity of the liquid storage barrel 302 is connected to the adjacent lower pipe 105, when the staff injects the solution into the adjacent filter cartridge 104, the two cavities in the liquid storage barrel 302 are both filled with solution, and the barrel body of the liquid storage barrel 302 is designed It is designed to be an easily disassembled structure, so that the staff can take out the filtered and collected bacteria in the liquid storage barrel 302, and an exhaust valve and a drain valve are provided in the lower cavity of the liquid storage barrel 302 (the exhaust valve and the drain valve are not shown in the existing device diagram). The exhaust valve on the liquid storage barrel 302 is used to smoothly discharge the air in the liquid storage barrel 302 when the solution enters the liquid storage barrel 302 during the initial replenishment of the solution into the adjacent filter cartridge 104, and the drain valve on the liquid storage barrel 302 is used to manually discharge the filtered solution stored in the liquid storage barrel 302 when the staff cleans the liquid storage barrel 302, and the staff manually discharges the filtered solution into the sterile collection barrel.

[0030] Further, see Figure 6-Figure 9 The interception component includes: a filter shell 304, which is rotatably connected to the adjacent liquid storage barrel 302; a moving member 305, which is slidably connected to the adjacent first piston rod 303, and the moving member 305 is used to drive the adjacent filter shell 304 to rotate; a first hydraulic telescopic rod 306, which is fixedly connected to the adjacent liquid storage barrel 302, and the telescopic end of the first hydraulic telescopic rod 306 is fixedly connected to the adjacent moving member 305.

[0031] Further, see Figure 7 and Fig. 9, also includes: a driving component, which is arranged in the shell 1 and is used to drive the two first hydraulic telescopic rods 306 to work in sequence, and the driving component includes: a driving cylinder 4, which is fixedly connected to the shell 1; a driving member 401, which is slidably connected to the shell 1, and the driving member 401 divides the driving cylinder 4 into a first cavity and a second cavity, the first cavity in the driving cylinder 4 is connected to one of the upper pipes 103, and the second cavity in the driving cylinder 4 is connected to the other upper pipe 103; a fixing frame 5, which is fixedly connected to the two liquid storage barrels 302, and a transmission gear is installed on the fixing frame 5, and the two first piston rods 303 are meshed with the transmission gear on the fixing frame 5 through a rack, and the driving member 401 is fixedly connected to one of the first piston rods 303.

[0032] In this embodiment, the specific shape of the filter shell 304 is not limited. The filter shell 304 can be circular or square. Its bottom and side walls can be retractable or can be a hard filter mesh structure. The filter shell 304 is used to filter the solution entering the liquid storage barrel 302 from the collection shell 3, and then further collect the bacteria collected in the collection shell 3. An arc groove is provided on the outer side of the movable member 305. When the movable member 305 moves up and down, the adjacent filter shells 304 rotate along the arc groove on the movable member 305. In this embodiment, the first hydraulic telescopic rod 306 is connected to the external hydraulic system to control the telescopic end of the first hydraulic telescopic rod 306 to extend or retract downward; the volume of the driving cylinder 4 is much smaller than that of the filter cylinder 104. The driving cylinder 4 is only used to detect the pressure difference at the filter cylinders 104 on both sides, and will not affect the pressure on both sides. The pressure difference of the filter cylinder 104 has a significant impact. The first cavity of the driving cylinder 4 is located on the upper side of the driving cylinder 4, and the second cavity of the driving cylinder 4 is located on the lower side of the driving cylinder 4. The first cavity of the driving cylinder 4 is connected to the upper connecting pipe 103 on the left, and the second cavity of the driving cylinder 4 is connected to the upper connecting pipe 103 on the right, and an exhaust valve and a drain valve are also provided in the driving cylinder 4 (the exhaust valve and the drain valve are not shown in the existing device diagram); the transmission gear on the fixed frame 5 is located between the two first piston rods 303, and the racks on the two first piston rods 303 are respectively located on the left and right sides of the transmission gear on the fixed frame 5. The transmission gear on the fixed frame 5 is used to drive the other first piston rod 303 to move in the opposite direction when one first piston rod 303 moves up and down. In this embodiment, the driving member 401 is fixedly connected to the first piston rod 303 on the right.

[0033] The working process is as follows: when the filter screen 2 on the left is filtering the solution, the driving blade fan 203 is driven by the adjacent solution to rotate clockwise (from top to bottom), and the driving blade fan 203 drives the two flow-pushing parts 202 to rotate clockwise (from top to bottom) through the pulley belt. When the left flow-pushing part 202 drives the solution to rotate, the solution drives the bacteria in the left filter screen 2 to rotate clockwise together. When the bacteria pass between the left collection filter screen 301 and the adjacent collection shell 3, they are intercepted by the left collection filter screen 301 and forced to stay between the collection filter screen 301 and the adjacent collection shell 3. At this time, the solution in the left upper pipe 103 is in the process of flowing, synchronously Entering into the first cavity of the driving cylinder 4, because there is no liquid in the upper pipe 103 on the right side in the initial state, the second cavity of the driving cylinder 4 is under air pressure, and the hydraulic pressure in the first cavity of the driving cylinder 4 is greater than the air pressure in the second cavity of the driving cylinder 4. The driving member 401 moves to the lowest side of the driving cylinder 4 under the action of the hydraulic pressure, and all the gas in the second cavity of the driving cylinder 4 is discharged. During the movement, the driving member 401 drives the first piston rod 303 on the right side to move to the lowest side of the right liquid storage barrel 302. When the first piston rod 303 on the right side moves, it drives the first piston rod 303 on the left side to move to the highest side of the left liquid storage barrel 302 through the transmission gear on the fixing frame 5.

[0034] When the solution enters the left filter cartridge 104 from the left upper connecting pipe 103, it simultaneously enters the upper cavity of the left liquid storage barrel 302 from the left collecting shell 3. At this time, less solution enters the upper cavity of the liquid storage barrel 302. After passing through the left filter screen 2, the solution moves downward to the left lower connecting pipe 105. At this time, the filtered sterile solution simultaneously enters the lower cavity of the left liquid storage barrel 302. After the solution enters the lower cavity of the liquid storage barrel 302, the gas in the lower cavity of the liquid storage barrel 302 is discharged outward through the adjacent exhaust valve.

[0035] When the filter screen 2 in the left filter cartridge 104 is clogged, the control terminal first suspends the liquid supply to the filter cartridge 104, the liquid pressure at the input pipe 101 is reduced, and part of the liquid in the filter cartridge 104 flows back into the input pipe 101 under the action of pressure, so that the liquid pressure of the left filter cartridge 104 is reduced, and the liquid pressure of the left filter cartridge 104 is prevented from being too high, which causes the left filter screen 2 to be damaged under the higher liquid pressure. The control terminal then injects hydraulic oil into the left first hydraulic telescopic rod 306, and the telescopic end of the first hydraulic telescopic rod 306 extends downward, and the telescopic end of the first hydraulic telescopic rod 306 drives the moving part 305 to move downward, and the moving part 30 The filter housing 304 is driven to rotate through the arc groove on the filter housing 304, and the filter housing 304 rotates to the lower side of the connection between the left liquid storage barrel 302 and the adjacent collecting housing 3. The filter housing 304 is ready to intercept the bacteria that enter the left liquid storage barrel 302 along with the liquid. Then the control terminal controls the first control valve 102 and the second control valve 106 to change the flow path of the solution, so that the filter screen 2 on the right side filters the solution. At this time, the solution enters the right filter cylinder 104 and the right liquid storage barrel 302 according to the same steps as entering the left filter cylinder 104 and the left liquid storage barrel 302. At the same time, the solution at the right upper pipe 103 flows into the second cavity of the driving cylinder 4.

[0036] When the solution flows into the second cavity of the driving cylinder 4, because the second cavity of the driving cylinder 4 is connected with the input pipe 101 through the right upper connecting pipe 103, and the control terminal supplies liquid to the input pipe 101 again, the solution pressure in the right upper connecting pipe 103 is greater than the solution pressure in the left right upper connecting pipe 103. Therefore, the liquid pressure in the second cavity of the driving cylinder 4 is greater than the liquid pressure in the first cavity of the driving cylinder 4. The driving member 401 moves upward under the push of the liquid pressure in the second cavity of the driving cylinder 4. During the movement of the driving member 401, the right first piston rod 303 is driven to move upward together. During the upward movement of the right first piston rod 303, the left first piston rod 303 is driven to move downward through the transmission gear on the fixing frame 5. During the downward movement of the left first piston rod 303, the solution in the lower cavity of the left liquid storage barrel 302 is squeezed into the left lower connecting pipe 105. At this time, because the left The side lower pipe 105 is disconnected from the second control valve 106, and all solutions can only pass through the left filter screen 2 upwards, and pass through the left collecting shell 3, and flow back to the upper cavity of the liquid storage barrel 302. When the solution passes through the left filter screen 2, the left filter screen 2 is backwashed, and the bacteria stuck on its mesh are washed down, so that the bacteria follow the solution through the left collecting shell 3 to flow into the left liquid storage barrel 302, and the bacteria between the left collecting shell 3 and the left collecting filter screen 301 follow the solution to flow into the left liquid storage barrel 302. In this process, the left push flow member 202 is always rotating, and the auxiliary solution rotates and flows in the filter screen 2, thereby increasing the force of the solution to drive the bacteria to flow. When the bacteria enter the upper cavity of the left liquid storage barrel 302, the bacteria are intercepted by the left filter shell 304. When the left first piston rod 303 moves to the lowest side, the solution in the left filter cartridge 104 stops flowing.

[0037] When the right filter screen 2 is blocked, the control terminal switches the solution flow path back to the left filter cartridge 104 according to the same process as above. At this time, the control terminal injects hydraulic oil into the right first hydraulic telescopic rod 306 while extracting the hydraulic oil in the left first hydraulic telescopic rod 306. The telescopic end of the left first hydraulic telescopic rod 306 retracts upward and resets. The telescopic end of the left first hydraulic telescopic rod 306 drives the adjacent moving part 305 to move upward. The moving part 305 drives the adjacent filter shell 304 and the bacteria therein to rotate synchronously through the arc groove on it. The left filter shell 304 returns to its initial position. The left filter shell 304 no longer intercepts the connection between the left liquid storage barrel 302 and the adjacent collecting shell 3. At this time, during the process of the left first piston rod 303 moving upward and resetting, the solution in the upper cavity of the liquid storage barrel 302 is all sterile solution, and most of the bacteria stuck at the mesh of the left filter screen 2 in the above process are collected in the left filter shell 304, so the left filter screen 2 is no longer in a blocked state.

[0038] When the staff has filtered all the solutions, the remaining solution in the liquid storage barrel 302 is discharged through the drain valve at the liquid storage barrel 302, and then the staff cleans the filter cartridge 104. The staff also takes out the two filter shells 304 and performs corresponding cleaning and disinfection.

[0039] Further, see Figure 3-Figure 10 , also includes: a pressure regulating component, which is arranged on the fixed frame 5, and is used to reduce the pressure in the upper connecting pipe 103 that needs recoil. The pressure regulating component includes: a negative pressure cylinder 501, which includes two, both of which are fixed to the fixed frame 5. A second piston rod 502 is slidably connected in the negative pressure cylinder 501. The negative pressure cylinder 501 is connected to the adjacent upper connecting pipe 103. The valve stem 1021 of the first control valve 102 is fixed to two fixing parts 503, and the fixing part 503 is slidably connected to the adjacent second piston rod 502.

[0040] Further, see Fig. 9 , the volume of the negative pressure cylinder 501 is greater than the volume of the driving cylinder 4.

[0041] Further, see Figure 6 , Figure 8 and Fig.10 , and also includes: a second hydraulic telescopic rod 504, having two, respectively fixed to the adjacent negative pressure cylinder 501, a spring is installed between the telescopic end of the second hydraulic telescopic rod 504 and its fixed part, the second piston rod 502 is used to squeeze the telescopic end of the adjacent second hydraulic telescopic rod 504, and the first hydraulic telescopic rod 306 is connected to the adjacent second hydraulic telescopic rod 504.

[0042] In this embodiment, the first control valve 102 is an existing structure, and the first control valve 102 is composed of a valve stem 1021, a valve housing and an electric control unit. The electric control unit drives the valve stem 1021 to rotate, so that the first control valve 102 is connected to different upper connecting pipes 103; the negative pressure cylinder 501 is used to extract the liquid in the upper connecting pipe 103 in an unused state, so as to further reduce the pressure at the upper connecting pipe 103, increase the pressure difference between the first cavity on the driving cylinder 4 and the second cavity on the driving cylinder 4, so as to ensure that the driving member 401 can move to the extreme position each time (move to the uppermost side of the stroke or the lowermost side of the stroke), thereby enhancing the force of the solution when recoiling the adjacent filter screen 2, and improving the recoil of the filter screen 2. cleaning effect; the volume of the negative pressure cylinder 501 is greater than the volume of the driving cylinder 4, thereby ensuring that the range of the negative pressure cylinder 501 to adjust the pressure in the adjacent filter cylinder 104 is greater than the range of change of the pressure in the filter cylinder 104 when the solution in the driving cylinder 4 is injected into the filter cylinder 104, ensuring that the negative pressure created by the negative pressure cylinder 501 effectively increases the pressure difference between the first cavity in the driving cylinder 4 and the second cavity in the driving cylinder 4. In this embodiment, the second hydraulic telescopic rod 504 replaces the hydraulic system in the above embodiment and is connected to the adjacent first hydraulic telescopic rod 306. Therefore, in this embodiment, the first hydraulic telescopic rod 306 is controlled by the adjacent second hydraulic telescopic rod 504, and is no longer controlled by the hydraulic system.

[0043] The working process is as follows: when the control terminal introduces the solution into the left filter cartridge 104, the left fixing piece 503 rotates to the lower side under the drive of the valve stem 1021, and the left fixing piece 503 drives the adjacent second piston rod 502 to move to the lower side. At this time, there is no solution in the left negative pressure cylinder 501, and the right fixing piece 503 rotates to the upper side under the drive of the valve stem 1021, and the right fixing piece 503 drives the adjacent second piston rod 502 to move to the upper side. At this time, there is gas in the right negative pressure cylinder 501. When the second piston rod 502 moves to the upper side, the telescopic end of the right second hydraulic telescopic rod 504 is squeezed to retract upward, and the spring on the right second hydraulic telescopic rod 504 is compressed and stored. The hydraulic oil in the right second hydraulic telescopic rod 504 enters the adjacent first hydraulic telescopic rod 306, and the telescopic end of the right first hydraulic telescopic rod 306 extends downward. At this time, the right filter shell 304 rotates to the interface between the right liquid storage barrel 302 and the right filter cartridge 104 under the drive of the adjacent first hydraulic telescopic rod 306 and the adjacent moving piece 305.

[0044] When the left filter screen 2 is clogged and the control terminal changes the flow direction of the solution, the control terminal controls the valve stem 1021 of the first control valve 102 to rotate, and the valve stem 1021 simultaneously drives the two fixing parts 503 thereon to rotate, the left fixing part 503 drives the adjacent part to move upward, and the right fixing part 503 moves downward, and the left fixing part 503 drives the adjacent second piston rod 502 to move to the uppermost side during the upward movement, and the left negative pressure cylinder 501 extracts the solution in the adjacent filter cylinder 104, further reducing the liquid pressure in the left filter cylinder 104, and further increasing the liquid pressure difference in the left and right filter cylinders 104, thereby ensuring that the driving member 401 moves to the limit state under the action of the pressure difference between the first cavity and the second cavity.

[0045] When the valve stem 1021 drives the two fixing members 503 thereon to rotate, the left fixing member 503 simultaneously squeezes the hydraulic oil in the left second hydraulic telescopic rod 504 into the left first hydraulic telescopic rod 306, so that the left filter housing 304 rotates to the interface between the right liquid storage barrel 302 and the right filter cartridge 104, and the right fixing member 503 drives the second piston rod 502 to move downward, and the telescopic end of the right second hydraulic telescopic rod 504 moves downward and resets under the action of the spring thereon, and the right first hydraulic telescopic rod 306 drives the adjacent filter housing 304 to move and reset. Through the above process, in the process of controlling the rotation of the valve stem 1021 in the first control valve 102, the valve stem 1021 drives the two first hydraulic telescopic rods 306 to complete the work in the above embodiment, without the need for an additional hydraulic system, thereby enhancing the scope of application of the present device.

[0046] Example 2: Based on Example 1, Figure 1-Figure 10 , a sterile filtration method for preparing a sterile culture medium, a sterile filtration device for preparing a sterile culture medium applied thereto, the specific method is as follows: S1: Control the first control valve 102 and the second control valve 106 to switch working states so that the two are connected to the same filter cartridge 104; S2: A solution is introduced into the input pipe 101. After the solution passes through the filter screen 2 in the filter cylinder 104 in use, it is discharged outward. The solution is simultaneously filled into the adjacent liquid storage barrel 302 and the driving cylinder 4. The flow-pushing member 202 drives the surrounding solution and bacteria to rotate, and the collection filter screen 301 collects the bacteria in the adjacent filter screen 2. S3: The driving member 401 moves under the pressure difference of the filter cartridges 104 on both sides, so that the first piston rod 303 in the working state moves to the upper side, and the other first piston rod 303 moves to the lower side. At the same time, the valve stem 1021 drives the two fixing members 503 to rotate, so that the second piston rod 502 in the working state moves to the lower side, and the other second piston rod 502 moves to the upper side. S4: When the filter screen 2 in the working state is clogged, the first control valve 102 and the second control valve 106 are controlled to switch the working state, so that the solution enters another filter cartridge 104 for filtration; S5: After the state of the first control valve 102 is switched, the two second piston rods 502 move toward each other, the two filter housings 304 are respectively driven by the adjacent second piston rods 502 to rotate, and the two first piston rods 303 move toward each other to backflush the clogged filter screen 2, and the filter housing 304 on the backflush side intercepts the bacteria that fall off the clogged filter screen 2; S6: After the solution is filtered, the filter cartridge 104, the liquid storage barrel 302 and the filter housing 304 are cleaned.

[0047] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the spirit of the present invention.

Claims

1. A sterilizing filtration device for preparing sterile culture medium, characterized in that: Included are: A housing (1), the housing (1) being fixedly connected to an input pipe (101), the input pipe (101) being fixedly connected to and in communication with a first control valve (102), the first control valve (102) being fixedly connected to and in communication with two upper connecting pipes (103), the upper connecting pipes (103) being fixedly connected to and in communication with a filter cartridge (104), the filter cartridge (104) being fixedly connected to and in communication with a lower connecting pipe (105), the two lower connecting pipes (105) being fixedly connected to and in communication with a second control valve (106), and the second control valve (106) being fixedly connected to and in communication with an output pipe (107); The filter screen (2) has two, and corresponds to the two upper pipes (103) one by one, and is respectively installed on the adjacent upper pipes (103) and located in the adjacent filter cartridge (104); the upper pipes (103) are rotatably connected to a flow-pushing member (202), and the flow-pushing member (202) is used to push the liquid and bacteria on the inner wall of the filter screen (2) to rotate at a low speed; The driving blade fan (203) is rotatably connected to the input pipe (101) and is provided with a speed measuring module for detecting its own rotation speed. The two flow-pushing members (202) and the driving blade fan (203) are both driven by pulleys and belts; A collection mechanism is arranged in the outer shell (1) and is used to collect bacteria in the filter screen (2).

2. A sterilizing filtration device for preparing sterile culture medium according to claim 1, characterized in that: The flow-promoting member (202) consists of a cylinder and circumferentially distributed vertical plates.

3. A sterilizing filtration device for preparing sterile culture medium according to claim 2, characterized in that: The collection mechanism includes: The collecting shells (3) are provided with two and correspond one to one with the two filter cartridges (104), and are respectively mounted on adjacent filter cartridges (104); the collecting shells (3) are fixedly connected to the filter screen (2); and the collecting shells (3) are fixedly connected with the collecting filter screen (301); There are two backflushing components, which correspond one to one with the two filter cartridges (104) and are both arranged in the housing (1) and are used to backflush the filter screen (2) when the adjacent filter screen (2) is clogged.

4. A sterilizing filtration device for preparing sterile culture medium according to claim 3, characterized in that: The recoil component comprises: A liquid storage barrel (302), the liquid storage barrel (302) being fixedly connected to the outer shell (1); A first piston rod (303) is slidably connected to the adjacent liquid storage barrel (302), the first piston rod (303) divides the liquid storage barrel (302) into an upper cavity and a lower cavity, the upper cavity of the liquid storage barrel (302) is connected to the adjacent collecting shell (3), and the lower cavity of the liquid storage barrel (302) is connected to the adjacent lower connecting pipe (105); An interception component is arranged on an adjacent liquid storage barrel (302) and is used to intercept and collect bacteria that enter the adjacent liquid storage barrel (302).

5. A sterilizing filtration device for preparing sterile culture medium according to claim 4, characterized in that: The interception components include: A filter housing (304) rotatably connected to an adjacent liquid storage barrel (302); A moving member (305) is slidably connected to the adjacent first piston rod (303), and the moving member (305) is used to drive the adjacent filter housing (304) to rotate; The first hydraulic telescopic rod (306) is fixedly connected in the adjacent liquid storage barrel (302), and the telescopic end of the first hydraulic telescopic rod (306) is fixedly connected to the adjacent moving member (305).

6. A sterilizing filtration device for preparing sterile culture medium according to claim 5, characterized in that: Also included are: A driving component is disposed in the housing (1) and is used to sequentially drive the two first hydraulic telescopic rods (306) to work, the driving component comprising: A driving cylinder (4) fixedly connected to the housing (1); a driving member (401) slidably connected to the housing (1), the driving member (401) dividing the driving cylinder (4) into a first cavity and a second cavity, the first cavity in the driving cylinder (4) being connected to one of the upper pipes (103), and the second cavity in the driving cylinder (4) being connected to the other of the upper pipes (103); The fixing frame (5) is fixedly connected to the two liquid storage barrels (302), a transmission gear is installed on the fixing frame (5), the two first piston rods (303) are meshed with the transmission gear on the fixing frame (5) through a rack, and the driving member (401) is fixedly connected to one of the first piston rods (303).

7. A sterilizing filtration device for preparing sterile culture medium according to claim 6, characterized in that: Also included are: A pressure regulating assembly is arranged on the fixing frame (5) and is used to reduce the internal pressure of the upper pipe (103) that needs to be recoiled. The pressure regulating assembly comprises: The negative pressure cylinder (501) includes two fixed parts, both of which are fixed to the fixing frame (5); a second piston rod (502) is slidably connected inside the negative pressure cylinder (501); the negative pressure cylinder (501) is connected to the adjacent upper connecting pipe (103); the valve stem (1021) of the first control valve (102) is fixed to two fixing parts (503); the fixing part (503) is slidably connected to the adjacent second piston rod (502).

8. A sterilizing filtration device for preparing sterile culture medium according to claim 7, characterized in that: The volume of the negative pressure cylinder (501) is greater than the volume of the driving cylinder (4).

9. A sterilizing filtration device for preparing sterile culture medium according to claim 8, characterized in that: Also included are: The second hydraulic telescopic rod (504) has two parts, which are respectively fixed to the adjacent negative pressure cylinder (501); a spring is installed between the telescopic end of the second hydraulic telescopic rod (504) and its fixed part; the second piston rod (502) is used to squeeze the telescopic end of the adjacent second hydraulic telescopic rod (504); the first hydraulic telescopic rod (306) is connected to the adjacent second hydraulic telescopic rod (504).

10. A sterilizing filtration method for preparing a sterile culture medium, applied to the sterilizing filtration device for preparing a sterile culture medium according to claim 9, characterized in that: The specific method is as follows: S1: Controlling the first control valve (102) and the second control valve (106) to switch their working states so that they are connected to the same filter cartridge (104); S2: A solution is introduced into the input pipe (101), and after the solution passes through the filter screen (2) in the filter cylinder (104) in use, it is discharged outward, and the solution is simultaneously filled into the adjacent liquid storage barrel (302) and the driving cylinder (4), the flow-pushing member (202) drives the surrounding solution and bacteria to rotate, and the collection filter screen (301) collects the bacteria in the adjacent filter screen (2); S3: The driving member (401) moves under the pressure difference between the filter cartridges (104) on both sides, so that the first piston rod (303) in the working state moves to the upper side, and the other first piston rod (303) moves to the lower side. At the same time, the valve stem (1021) drives the two fixing members (503) to rotate, so that the second piston rod (502) in the working state moves to the lower side, and the other second piston rod (502) moves to the upper side. S4: when the filter screen (2) in the working state is clogged, the first control valve (102) and the second control valve (106) are controlled to switch the working state, so that the solution enters another filter cartridge (104) for filtration; S5: After the state of the first control valve (102) is switched, the two second piston rods (502) move towards each other, the two filter shells (304) are respectively driven by the adjacent second piston rods (502) to rotate, and the two first piston rods (303) move towards each other to backwash the clogged filter screen (2), and the filter shell (304) on the backwashing side intercepts bacteria that fall off the clogged filter screen (2); S6: After the solution is filtered, the filter cartridge (104), the liquid storage barrel (302) and the filter housing (304) are cleaned.

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