Soil reconstruction method based on carbonaceous slate waste slag curing

Abstract

The invention discloses a soil reconstruction method based on carbonaceous slate waste slag curing, and belongs to the technical field of slope ecological restoration. The raw materials comprise carbonaceous rock plate residues, nutrient soil, a microbial fertilizer and a water-retaining polymer. The raw materials are mixed and then applied to an engineering slope, and then herbaceous plant seeds are planted in the planting matrix; the microbial fertilizer is prepared by mixing bacillus amyloliquefaciens, bacillus mucilaginosus and bacillus subtilis according to the mass ratio of 1: 1: (1-4); the carbonaceous slate is matched with the nutrient soil, the microbial fertilizer and the water-retaining polymer for use, so that the physicochemical properties of the matrix can be effectively improved, plant growth is promoted, and microbial communities are enriched.

Description

Technical Field

[0001] The invention belongs to the technical field of slope ecological restoration, and in particular relates to a soil reconstruction method based on the maturation of carbonaceous slate waste. Background Art

[0002] In road construction projects, such as excavation and tunnel engineering, a large amount of waste slag will be generated. As the proportion of bridges and tunnels increases, the amount of waste slag generated is also increasing. At present, the utilization rate of these resources is less than 10%. These slag bodies are relatively loose and broken. Random stacking will aggravate soil erosion and soil erosion, and may even become a triggering factor for geological disasters such as collapse, landslides, and mud-rock flows. At the same time, slag will also occupy land resources. During the foundation excavation process, different geological layers will be encountered, including carbonaceous slate, which is excavated to become part of the engineering slag. Carbonaceous slate is black or gray-black, with dense lithology, clear slabs, straight edges and corners, and smooth slabs. It is mainly formed by clay, silt, and sedimentary rocks through slight metamorphism. It contains a large amount of quartz, boron carbide, mica, and some sulfides, phosphates, etc.

[0003] Recycling waste residue is a necessary measure to improve the environment. For example, adding soil conditioners can increase its nutrient content, improve soil structure, and make it a planting matrix that is conducive to plant growth. Using carbonaceous slate as a planting matrix for plant growth can reduce the need for stacking, relieve land pressure, eliminate safety hazards caused by stacking, and reduce soil erosion. However, related research on the resource utilization of waste residue needs further exploration. Summary of the invention

[0004] In view of the above technical problems, the present invention proposes a soil reconstruction method based on the maturation of carbonaceous slate waste.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A soil reconstruction method based on the maturation of carbonaceous slate waste, the raw materials include carbonaceous slate waste, nutrient soil, microbial fertilizer and water-retaining polymer; the raw materials are mixed and applied to the engineering slope, and then herbaceous plant seeds are planted in the planting matrix; the microbial fertilizer is mixed with Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:(1-4).

[0007] Carbonaceous slate slag has good stability and fertility, can provide nutrients and water for plants, help plant growth, and also improve soil structure. Nutrient soil is mainly composed of humus, organic matter, etc., which can provide plants with essential nutrients and promote plant growth. Microbial fertilizers can accelerate the decomposition of organic matter in carbonaceous slate slag, increase the release of nutrients, and thus promote plant growth. Water-retaining polymers can increase the water retention capacity of the soil, reduce evaporation, increase soil moisture, and enable plants to grow in a drier environment. The present invention effectively utilizes the soil resources of the slope through synergistic effects, repairs the ecological environment of the slope, prevents soil erosion, and protects land resources.

[0008] Furthermore, based on the total mass of the carbonaceous slate slag and the nutrient soil being 10, the mass fraction of the carbonaceous slate slag is 7-10, and the remainder is the nutrient soil.

[0009] Furthermore, the particle size of the carbonaceous slate slag is ≤5 mm.

[0010] Furthermore, the nutrient soil is composed of straw fermented soil, organic earthworm castings, plant ash and horticultural substrate; wherein the horticultural substrate is composed of vermiculite, perlite, carbonate and root powder.

[0011] Furthermore, the mass ratio of the total mass of the carbonaceous slate slag and the nutrient soil to the mass of the microbial fertilizer is 1000:(1-10).

[0012] Furthermore, the microbial fertilizer is formed by mixing Bacillus amyloliquefaciens, Bacillus gelatinosa and Bacillus subtilis in a mass ratio of 1:1:(1-4).

[0013] Furthermore, the ratio of the total mass of the carbonaceous slate slag and the nutrient soil to the water-retaining polymer is 1000 g: 80 mL.

[0014] Furthermore, the mass concentration of the water-retaining polymer is 0.8-1%.

[0015] Furthermore, the herbaceous plant seeds include one or more of Elymus dactylis, Fescue arundinaceus, Lolium perenne and alfalfa.

[0016] Furthermore, the engineering slope is an engineering slope in arid and cold regions.

[0017] Compared with the prior art, the present invention has the following advantages and technical effects:

[0018] The carbonaceous slate of the present invention can be used in combination with nutrient soil, microbial fertilizer and water-retaining polymer to effectively improve the physical and chemical properties of the matrix, promote plant growth and enrich the microbial community. The present invention finds that carbonaceous slate can be used as a planting matrix for resource utilization. The method provided by the present invention will provide theoretical and technical basis for ecological restoration of mining areas, land reclamation of quarries and vegetation restoration. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings:

[0020] Figure 1 It is a process flow chart of the present invention;

[0021] Figure 2 Effects of planting matrix on soil physical and chemical properties in experimental groups T1-T9 and control group 1, including (a) pH; (b) EC; (c) water content; (d) NH4 + -N; (e) NO3 - -N; (f) TN; (g) TP; (h) SOC; (i) effective P; (j) fast-acting K;

[0022] Figure 3 Effects of planting medium on plant growth indicators in experimental groups T1-T9 and control group 1, including (a) chlorophyll content; (b) plant height; (c) root length; (d) root surface area; (e) root volume; (f) average root diameter;

[0023] Figure 4 The effects of planting substrates on soil enzyme activities in experimental groups T1-T9 and control group 1, including (a) soil alkaline protease (S-ALPT); (b) soil amylase (S-AL); (c) soil catalase (S-CAT); (d) soil alkaline phosphatase (S-AKP);

[0024] Figure 5 Effects of planting matrix on soil microorganisms in experimental groups T1-T9 and control group 1, including (a) α-diversity index; (b) species composition heat map at genus level; (c) OUT Venn diagram; (d) signal molecules AHL&AIP. DETAILED DESCRIPTION

[0025] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0026] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0027] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0028] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0029] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0030] The following embodiment of the present invention provides a soil reconstruction method based on the maturation of carbonaceous slate waste, comprising the following steps:

[0031] Carbonaceous slate slag is used as the main raw material of the planting matrix, and nutrient soil, microbial fertilizer and water-retaining polymer are used as auxiliary materials of the planting matrix; the raw materials and auxiliary materials are mixed and applied to the engineering slope, and then herbaceous plant seeds are planted in the planting matrix to complete the ecological restoration of the engineering slope.

[0032] In some embodiments, the mass ratio of the carbonaceous slate slag to the nutrient soil is (7-10):(3-0). Exemplarily, in the following preferred embodiments of the present invention, the mass ratio of the carbonaceous slate slag to the nutrient soil includes 7:3, 9:1, 10:0 or any range between the aforementioned ratios.

[0033] In some embodiments, the particle size of the carbonaceous slate slag is ≤5 mm.

[0034] In some embodiments, the nutrient soil is composed of fermented straw soil, organic earthworm castings, plant ash and horticultural substrate; wherein the horticultural substrate is composed of vermiculite, pearlite, carbonic acid and root powder. It can be made by yourself or purchased. The nutrient soil used in the embodiment of the present invention is a purchased product purchased from: Jurong Kaihong Plastic Industry Co., Ltd.

[0035] In some embodiments, the mass ratio of the total mass of the carbonaceous slate slag and the nutrient soil to the microbial fertilizer is 1000:(1-10). Exemplarily, in the following preferred embodiments of the present invention, the mass ratio of the carbonaceous slate slag and the nutrient soil includes 1000:1, 1000:5, 1000:10 or any range between the aforementioned ratios.

[0036] In some embodiments, the microbial fertilizer is mixed with Bacillus amyloliquefaciens, Bacillus jelly-like and Bacillus subtilis in a mass ratio of 1: 1: (1-4). The strains used are all purchased products on the market, purchased from: Beihai Qiangxing Biological Mall. Exemplary, in the following preferred embodiments of the present invention, the Bacillus amyloliquefaciens, Bacillus jelly-like and Bacillus subtilis are in a mass ratio of 1: 1: 1, 1: 1: 4 or any range between the aforementioned ratios. And whether the microbial fertilizer has been inactivated is also one of the influencing factors. In the following embodiments of the present invention, it is not emphasized that the inactivation treatment is performed, and it is assumed that the high-pressure inactivation treatment is not performed.

[0037] In some embodiments, the ratio of the total mass of the carbonaceous slate slag and the nutrient soil to the water-retaining polymer is 1000 g:80 mL.

[0038] In some embodiments, the mass concentration of the water-retaining polymer is 0.8-1%. The water-retaining polymer used in the present invention is the product in patent application CN105925271A, and the specific preparation method of the water-retaining polymer with a mass concentration of 15% is:

[0039] a. Stir and dissolve sodium carboxymethyl cellulose and water in a mass ratio of 1:20 to obtain a sodium carboxymethyl cellulose solution;

[0040] b. Stirring and dissolving anionic polyacrylamide and water at a mass ratio of 1:100 to obtain anionic polyacrylamide solution;

[0041] c. Stir and dissolve ferrous chloride and water at a mass ratio of 1:50 to obtain a ferrous chloride solution;

[0042] d. At room temperature, sequentially add sodium carboxymethyl cellulose solution, anionic polyacrylamide solution and ferrous chloride solution into water and mix them to obtain a water-retaining polymer.

[0043] If you want to obtain water-retaining polymers with different mass concentrations, you can dilute them by adding ultrapure water. For example, in the following preferred embodiments of the present invention, the mass concentration of the water-retaining polymer can be selected to be 0.8%, 1% or any value between the above ratios.

[0044] In some embodiments, the herbaceous plant seeds include one or more of Elymus dahliae, Tall Fescue, Lolium perenne and Alfalfa. For example, in the following preferred embodiments of the present invention, Alfalfa seeds are selected as an example for experimental discussion.

[0045] In some embodiments, the engineering slope is an engineering slope in arid and cold regions.

[0046] The raw materials used in the present invention are all purchased from the market.

[0047] In the following examples of the present invention, carbonaceous slate from a road construction waste dump was selected as raw material, and the results of the physical and chemical property tests were as follows:

[0048] pH: 9.7

[0049] Electrical conductivity (EC) (ms / cm): 0.32

[0050] Natural moisture content (%): 3.87

[0051] Available potassium (mg / kg): 61.36

[0052] Available phosphorus (mg / kg): 1.08

[0053] Organic carbon (g / kg): 0.43

[0054] Ammonium nitrogen (mg / kg): 0.36

[0055] Nitrate nitrogen (mg / kg): 0.68

[0056] Total nitrogen (g / kg): 0.17

[0057] Total phosphorus (g / kg): 0.22.

[0058] The technical solution of the present invention is further illustrated by the following embodiments.

[0059] Example 1

[0060] A soil reconstruction method based on the maturation of carbonaceous slate waste (see Figure 1 ), including the following steps:

[0061] The experiment was conducted in a greenhouse with an average temperature of 25°C and a humidity of 50% for 84 days. On January 11, 2024, 30 alfalfa seeds were sown manually in pots with a diameter of 15.5 cm and a height of 10.5 cm. Alfalfa seeds were disinfected with 10% hydrogen peroxide for 10 min before sowing and then rinsed with deionized water. In order to ensure the normal growth of the plants, the irrigation water volume was maintained at 80% of the water content in the control area (CK), equivalent to 88 grams per irrigation. No fertilizer was applied to the plants during the growth period. Orthogonal test was used, and four factors were considered: the mass ratio of carbonaceous slate slag and nutrient soil (7:3, 9:1, 10:0; SS, named A1, A2, A3, respectively), water-retaining polymer concentration (0.8%, 1%, 0%, WP, named B1, B2, B3, respectively), microbial fertilizer application ratio (1:1:1, 1:1:3, inactivated 1:1:1 mixed bacterial fertilizer, RF, named C1, C2, C3, respectively), and microbial fertilizer application amount (1, 5, 10 g kg -1 , FU, named D1, D2, D3 respectively). The orthogonal test obtained 9 different groups, named T1-T9, with pure carbonaceous slate slag as the control group (CK). Each group was set up as 3 parallel groups. The specific plan of the planting matrix is ​​set as follows:

[0062] Experimental group T1:

[0063] The mass ratio of carbonaceous slate slag to nutrient soil is 7:3, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0064] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:1; the added amount is 1g;

[0065] Ultrapure water was added to the water-retaining polymer with a mass concentration of 15% to obtain a water-retaining polymer with a mass concentration of 0.8%. The added amount was 80 mL.

[0066] Experimental group T2

[0067] The mass ratio of carbonaceous slate slag to nutrient soil is 7:3, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0068] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:4; the added amount is 5g;

[0069] Ultrapure water was added to the water-retaining polymer with a mass concentration of 15% to obtain a water-retaining polymer with a mass concentration of 1%. The added amount was 80 mL.

[0070] Experimental group T3

[0071] The mass ratio of carbonaceous slate slag to nutrient soil is 7:3, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0072] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinosa and Bacillus subtilis in a mass ratio of 1:1:1; the added amount is 10g.

[0073] Experimental group T4

[0074] The mass ratio of carbonaceous slate slag to nutrient soil is 9:1, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0075] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:4; the added amount is 10g;

[0076] Ultrapure water was added to the water-retaining polymer with a mass concentration of 15% to obtain a water-retaining polymer with a mass concentration of 0.8%. The added amount was 80 mL.

[0077] Experimental group T5

[0078] The mass ratio of carbonaceous slate slag to nutrient soil is 9:1, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0079] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:1; the added amount is 1g;

[0080] Ultrapure water was added to the water-retaining polymer with a mass concentration of 15% to obtain a water-retaining polymer with a mass concentration of 1%. The added amount was 80 mL.

[0081] Experimental group T6

[0082] The mass ratio of carbonaceous slate slag to nutrient soil is 9:1, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0083] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinosa and Bacillus subtilis in a mass ratio of 1:1:1; the added amount is 5g.

[0084] Experimental group T7

[0085] The mass ratio of carbonaceous slate slag to nutrient soil is 10:0, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0086] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:1; the added amount is 5g;

[0087] Ultrapure water was added to the water-retaining polymer with a mass concentration of 15% to obtain a water-retaining polymer with a mass concentration of 0.8%. The added amount was 80 mL.

[0088] Experimental group T8

[0089] The mass ratio of carbonaceous slate slag to nutrient soil is 10:0, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0090] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinus and Bacillus subtilis in a mass ratio of 1:1:1; the added amount is 10g;

[0091] Ultrapure water was added to the water-retaining polymer with a mass concentration of 15% to obtain a water-retaining polymer with a mass concentration of 1%. The added amount was 80 mL.

[0092] Experimental group T9

[0093] The mass ratio of carbonaceous slate slag to nutrient soil is 10:0, and the total amount of carbonaceous slate slag and nutrient soil added is 1000g;

[0094] The microbial fertilizer is a mixture of Bacillus amyloliquefaciens, Bacillus gelatinosa and Bacillus subtilis in a mass ratio of 1:1:4; the added amount is 1g.

[0095] Control group 1 (CK)

[0096] 1000 g of carbonaceous slate slag was used as the entire planting medium.

[0097] Table 1 Parameter changes of experimental groups T1-T9

[0098]

[0099]

[0100] 1. Physical and chemical properties of soil

[0101] pH: acidity meter;

[0102] Electrical conductivity (EC): The electrical conductivity of the soil was measured using an acidometer;

[0103] Moisture content: The soil moisture content is measured by the ring knife method;

[0104] Organic carbon: potassium dichromate oxidation-spectrophotometry;

[0105] Nitrate nitrogen (NO3 - -N): hydrazine sulfate reduction method;

[0106] Ammonium nitrogen (NH4 + -N): Sodium salicylate method;

[0107] Available potassium (Available P): ammonium acetate extraction-flame atomic absorption spectrometry;

[0108] Available P: phosphomolybdenum blue method;

[0109] Total nitrogen (TN): sulfuric acid digestion-sodium salicylate method;

[0110] Total phosphorus (TP): sulfuric acid digestion-molybdenum antimony resistance method.

[0111] In most cases, the addition of exogenous substances increased the values ​​of these soil physical and chemical properties. Figure 2 As shown in the results (data are expressed as mean ± standard deviation of three replicates. Different lowercase letters indicate statistically significant differences (p<0.05), the same below), the soil physical and chemical properties were significantly different (p<0.05). The soil pH value increased with the increase of the proportion of carbonaceous slate, and there were significant differences among the groups (p<0.05). Water-retaining polymers and microbial fertilizers can significantly reduce the pH (p<0.05), because water-retaining polymers can increase the looseness of the soil, good soil structure helps to reduce the salinization of the soil, and the microbial activity in the microbial agent can produce acidic substances and regulate the acid-base balance of the soil. The amount of microbial agent has a significant effect on the conductivity value (p<0.05), and the microbial agent promotes the decomposition and mineralization of organic matter in the soil. With the increase of the proportion of carbonaceous slate, the water content is significantly reduced (p<0.05), because the gaps between the carbonaceous slate particles are large and drain freely. The ammonia nitrogen content increased due to the addition of materials. Several materials promoted the fixation and release of nitrogen in the soil, especially the activity of nitrogen-fixing bacteria, which led to an increase in the ammonia nitrogen content. The nitrate nitrogen content decreased with the addition of materials. The addition of these materials may have changed the activity of soil microorganisms and enhanced denitrification, so that nitrate nitrogen was converted into nitrogen gas or other forms, resulting in a significant decrease in nitrate nitrogen content (p < 0.05). The soil-slag ratio had a greater impact on the total nitrogen content because carbonaceous slate is rich in organic matter, which can release nitrogen during decomposition. The total phosphorus content was greatly affected by the proportion of bacterial agents because phosphate-solubilizing bacteria can effectively promote the transformation and release of phosphorus in the soil. The addition of nutrient soil significantly increased the organic carbon content in the soil (p < 0.05). With the increase in the amount of bacterial agents, the organic carbon content decreased significantly (p < 0.05); the addition of bacterial agents significantly increased the effective phosphorus content (p < 0.05). When the dosage of the microbial agent was 5g, the available phosphorus content was significantly higher than that of other treatment groups (p < 0.05), because more microbial agents may cause an imbalance in the soil environment, affect the activity of microorganisms, and thus slow down the conversion rate of phosphorus; the available potassium content increased significantly with the increase in the amount of waste slag added (p < 0.05), and the addition of carbonaceous slate provided additional potassium to the soil.

[0112] 2. Plant growth indicators

[0113] 84 days after sowing, alfalfa plants were harvested and their plant height was measured with a ruler. Chlorophyll in the leaves was extracted by ethanol extraction, and the absorbance was measured to calculate the chlorophyll content. A root scanner was used to measure plant root length, root surface area, root volume and average root diameter.

[0114] Figure 3 The results reflect the effects of each treatment on plant growth indicators. The addition of microbial agents can significantly increase the chlorophyll content of alfalfa leaves (p < 0.05). Microorganisms promote root growth and nutrient absorption, regulate plant physiological and biochemical processes, and improve plant stress resistance, which significantly promotes the photosynthesis of alfalfa. Each treatment can increase plant height. The addition of water-retaining polymers and microbial agents significantly improved several root characteristics of alfalfa (p < 0.05). These materials can improve the soil environment, promote the formation of soil aggregates, improve soil fertility, and provide alfalfa roots with richer nutrients and more suitable growth conditions.

[0115] 3. Determination of soil enzyme activity

[0116] Soil alkaline protease, soil amylase, soil catalase and soil alkaline phosphatase were all determined using enzyme-linked immunosorbent assay kits using a microplate reader.

[0117] Soil enzyme activity reflects the intensity and direction of various biochemical processes in the soil. It is also unified with the environment and is easily affected by physical, chemical and biological factors in the environment. Figure 4 In the experiment, several treatments increased the activities of these soil enzymes, and the application of microbial agents had a significant effect on the activities of several enzymes (p < 0.05), because microbial activity optimized soil conditions, provided a more suitable growth environment for microorganisms that could secrete these enzymes, promoted their proliferation, and thus increased the activities of various enzymes. Soil alkaline protease can catalyze the hydrolysis of protein substances, participate in the decomposition and transformation of soil organic nitrogen, and play a key role in soil nitrogen metabolism and plant nutrient absorption. Several treatments significantly increased catalase activity (p < 0.05). The main function of soil catalase is to catalyze the decomposition of hydrogen peroxide into water and oxygen, maintain soil redox balance, and promote soil health and plant growth. As the proportion of carbonaceous slate slag decreases, the activity of alkaline phosphatase increases. This is because the addition of carbonaceous slate slag will reduce soil porosity, thereby affecting the activity of soil microorganisms and inhibiting the activity of alkaline phosphatase. Therefore, when the proportion of carbonaceous slate slag decreases, the soil maintains a good pore structure, which is conducive to the growth and reproduction of soil microorganisms. Soil alkaline phosphatase can catalyze the mineralization of organic phosphorus compounds in the soil and release inorganic phosphorus for plant absorption and utilization.

[0118] 4. Microbial diversity analysis and AIP and AHL signaling molecule determination

[0119] Soil microbial biomass, activity and diversity are indicators of soil fertility and ecosystem productivity. Figure 5 As shown in the figure, Good's coverage is greater than 98%, which can reflect the structure of the soil microbial community. The Chao1 index is significantly positively correlated with the richness of the microbial community, and the Shannon index and Simpson index are positively correlated with the diversity index of the microbial community. The Chao1 index of each experimental group is higher than that of the control group, indicating that the added exogenous substances have a positive effect on the microbial richness of the implant matrix. All samples contained 142 identical OTUs, and the OTUs of each experimental group were significantly higher than those of the control group. In addition, the species composition heat map at the genus level shows that these microorganisms have functions in carbon cycle, organic matter decomposition, nitrogen fixation, etc. The microorganisms in the T8 experimental group often exist in saline-alkali environments, and the pH and conductivity values ​​of the soil in this experimental group are high, belonging to saline-alkali soil.

[0120] AIP and AHL are effector signal molecules that affect biofilm formation and have the function of monitoring microbial population density. Microorganisms can coordinate in complex environments by controlling the cycle of signal molecule synthesis, thereby improving their survival ability. This study shows that the addition of exogenous substances can change the concentration of signal molecules, promote bacterial quorum sensing, and thus affect the microbial ecological characteristics of the soil.

[0121] 5. Screening and optimization of substrate ratio

[0122] In order to convert each index into a comprehensive variable for analysis, the present invention adopts the principal component analysis method to perform weight analysis on the variables, and then adopts the TOPSIS analysis method to obtain the final score ranking of each experimental group. The TOPSIS analysis method is to select the best planting matrix that is closest to the ideal optimal solution and far away from the worst solution by comparing the distance between each planting matrix scheme and the ideal optimal and worst solutions. As shown in Table 2.

[0123] Table 2 TOPSIS evaluation calculation results

[0124]

[0125]

[0126] As can be seen from Table 2, the final scores from high to low are: T4>T1>T2>T3>T6>T5>T8>T7>T9>CK. According to the scores, group T4 was determined as the preferred solution, that is, the mass ratio of carbonaceous slate slag and nutrient soil was 9:1; the mass concentration of water-retaining polymer was 0.8%; the ratio of bacterial agent was 1:1:4; and the dosage of bacterial agent was 10g.

[0127] The above are only preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A soil reconstruction method based on the maturation of carbonaceous slate waste, characterized in that: The raw materials include carbonaceous rock slag, nutrient soil, microbial fertilizer and water-retaining polymer; the raw materials are mixed and applied to the engineering slope, and then herbaceous plant seeds are planted in the planting matrix; The microbial fertilizer is prepared by mixing bacillus amyloliquefaciens, bacillus gelatinous and bacillus subtilis in a mass ratio of 1:1:(1-4).

2. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 1 is characterized in that: Taking the total mass of the carbonaceous slate slag and the nutrient soil as 10, the mass fraction of the carbonaceous slate slag is 7-10, and the remainder is the nutrient soil.

3. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 2 is characterized in that: The particle size of the carbonaceous slate slag is ≤5mm.

4. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 2 is characterized in that: The nutrient soil is composed of fermented straw soil, organic earthworm manure, plant ash and horticultural substrate; wherein the horticultural substrate is composed of vermiculite, pearlite, carbonic acid and root powder.

5. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 1 is characterized in that: The mass ratio of the total mass of the carbonaceous slate slag and the nutrient soil to the mass of the microbial fertilizer is 1000:(1-10).

6. The soil reconstruction method based on carbonaceous slate waste slag maturation according to claim 1, characterized in that: The ratio of the total mass of the carbonaceous slate slag and the nutrient soil to the water-retaining polymer is 1000 g: 80 mL.

7. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 7 is characterized in that: The mass concentration of the water-retaining polymer is 0.8-1%.

8. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 1, characterized in that: The herbaceous plant seeds are selected from one or more of Elymus dactylis, tall fescue, ryegrass and alfalfa.

9. The soil reconstruction method based on the maturation of carbonaceous slate waste according to claim 1, characterized in that: The engineering slope is an engineering slope in arid and cold areas.

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