Method for carrying out soil improvement and vegetation reconstruction on saline-alkali spots by utilizing puccinellia divaricata

Abstract

The invention discloses a method for carrying out soil improvement and vegetation reconstruction on saline-alkali spots by utilizing puccinellia tenuifolia. The method provided by the invention comprises the following steps: (1) applying an organic fertilizer to the saline-alkali spot soil to be improved, then turning over, and then applying an amino acid water-soluble fertilizer; (2) after the step (1) is completed, digging holes, then transplanting puccinellia tenuifolia plants into the holes, then covering soil, restoring vegetation through the growth of puccinellia tenuifolia, and completing the improvement of the saline-alkali spot soil; the depth of each hole is 15 cm, the soil level is lower than the ground level after soil covering, and the distance between the soil level and the ground level is 3 cm. Vegetation of the saline-alkali soil can be rapidly recovered, the productivity of the grassland where the saline-alkali soil is located is improved, and the ecological environment of the grassland is improved.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology and relates to a method for improving soil and re-vegetating saline-alkali spots by using Puccinellia tenuifolia. Background Art

[0002] The formation of saline-alkali spots is mainly due to the fact that the evaporation of soil moisture is greater than the precipitation, and the groundwater moves upward, causing the underground salt to transfer to the surface, thus forming saline-alkali soil. Excessive salt accumulation makes it impossible for surface vegetation to grow. If it is not treated, the surface will be covered with vegetation, which will further increase the surface evaporation and surface temperature, thereby further expanding the area of ​​saline-alkali spots. The expansion of the area of ​​saline-alkali spots not only seriously affects the grassland ecological environment, but also leads to a shortage of high-quality forage, and the contradiction between grass and livestock becomes increasingly prominent, which restricts the sustainable development of local grassland animal husbandry and the grassland ecological environment.

[0003] Puccinellia is a top-quality forage grass with rich nutrition and good palatability. It is favored by livestock and has important ecological and economic value. It is highly adaptable and cold-resistant and salt-alkali tolerant. Puccinellia usually grows best when the soil pH is around 9, but if the soil pH exceeds 9.5, it cannot be planted by direct sowing. It usually has problems such as low germination rate, weak growth in the seedling stage, and failure to regreen in the second year. The traditional method of using imported soil to press alkali to plant Puccinellia requires a large amount of soil, and soil resources are limited, which makes it difficult to implement. Summary of the invention

[0004] The invention aims to provide a method for improving soil and re-vegetating saline-alkali spots by using Puccinellia serrata.

[0005] The present invention provides a method for improving soil and revegetating saline-alkali spots, comprising the following steps: (1) Apply organic fertilizer to the saline-alkali soil to be improved, then till the soil, and then apply amino acid water-soluble fertilizer; (2) After completing step (1), dig a hole, transplant the alkali grass plants into the hole, and then cover the hole with soil, so that the vegetation is restored through the growth of the alkali grass and the saline-alkali soil is improved; the depth of the hole is 15 cm, and after covering the hole with soil, the soil level is lower than the ground level and the distance between the soil level and the ground level is 3 cm.

[0006] The amount of amino acid water-soluble fertilizer applied is 0.02L / m 2 .

[0007] The amount of organic fertilizer applied is 3kg / m 2 .

[0008] The Puccinellia repens plants used for transplanting are Puccinellia repens plants in the seedling stage.

[0009] The Puccinellia repens plants used for transplanting are those with a plant height ≥10 cm.

[0010] The Puccinellia repens plants used for transplanting were Puccinellia repens plants that had been sown for 30 days.

[0011] The covering soil covers the soil dug out from the hole.

[0012] When transplanting, the spacing between plants and rows of Puccinellia sinensis was 30 cm.

[0013] After transplanting, normal cultivation and management are carried out (watering is carried out according to soil moisture conditions within 30 days). When the seedlings are 30cm tall, they can be mowed appropriately, leaving a stubble height of 10cm.

[0014] The preparation method of the Puccinellia tenuifolia plants for transplanting comprises the following steps: taking Puccinellia tenuifolia seeds, breaking the dormancy of the seeds, sowing the seeds in a seedling pot filled with a culture matrix, and then covering the seeds with the culture matrix.

[0015] The breaking of seed dormancy comprises the following steps: soaking the Puccinellia tenuifolia seeds in water at room temperature for 3 hours, and then drying them. The drying process may be drying at 37° C. for 12 hours or drying at room temperature for 24 hours.

[0016] In the step ②, the covering thickness of "covering the seeds with the culture matrix" is less than 0.8 cm.

[0017] In step ②, the watering management method is as follows: water the culture medium thoroughly before sowing, water normally after sowing, stop watering 3-5 days before transplanting, and water thoroughly on the day of transplanting.

[0018] The sowing time is: sowing after reaching "average daily temperature ≥ 8°C and minimum temperature ≥ 5°C" for 5 consecutive days.

[0019] The tillage refers to: deep tillage (depth of 30 cm) and then harrowing (to make the soil fine and flat).

[0020] When the amino acid water-soluble fertilizer is applied, the amino acid water-soluble fertilizer is used in the form of an amino acid water-soluble fertilizer solution.

[0021] The amino acid water-soluble fertilizer solution is obtained by diluting the amino acid water-soluble fertilizer with water to 500 times the volume.

[0022] The amino acid water-soluble fertilizer is applied by drip irrigation.

[0023] Specifically, the amino acid water-soluble fertilizer is a liquid preparation.

[0024] Specifically, the amino acid water-soluble fertilizer is the following product: Registration No.: Agricultural Fertilizer (2017) Approval No. 6147.

[0025] Specifically, the amino acid water-soluble fertilizer is the following product: amino acid ≥ 100g / L, calcium ≥ 30g / L.

[0026] Specifically, the amino acid water-soluble fertilizer is the following product: the seller is Tianjin Yuchuan Jinhong Environmental Protection Technology Co., Ltd.

[0027] Specifically, the amino acid water-soluble fertilizer is the following product: the manufacturer is Tianjin New Age Agricultural Technology Co., Ltd., and the product name is "Antifreeze Foliar Fertilizer (Containing Amino Acid Water-soluble Fertilizer)".

[0028] The quality control standard of the Puccinellia sinensis seeds is "germination rate above 75% and purity above 90%".

[0029] The culture medium is loam.

[0030] Specifically, the culture medium is loam obtained from farmland.

[0031] Specifically, the pH value of the loam is 6-7, and the conductivity is 0.3-0.5 ms / cm.

[0032] Specifically, the pH value of the loam is 6.28, and the electrical conductivity is 0.43 ms / cm.

[0033] The improvement is embodied in at least one of the following (a) to (i): (a) Lowering soil pH; (b) reduce soil electrical conductivity; (c) reduce the exchangeable sodium ion content of soil; (d) reduce the exchangeable potassium ion content in the soil; (e) Reducing the available phosphorus content in the soil; (f) Increase the exchangeable calcium ion content in the soil; (g) increasing soil organic matter content; (h) increasing the soil alkaline nitrogen content; (i) Increase the available potassium content in the soil.

[0034] The lowering of soil pH may be lowering soil pH by 7%.

[0035] The 7% reduction was compared to the control method (CK3).

[0036] The lowering of soil pH may be lowering the soil pH to below 10 (eg 9.9).

[0037] The lowering of soil pH may be to lower the soil pH from 10.7 to below 10 (eg 9.9).

[0038] The reducing soil electrical conductivity may be reducing soil electrical conductivity by 72%. The reduction was 72% compared to the control method (CK3).

[0039] The reducing soil conductivity may be reducing the soil conductivity to below 0.8 ms / cm (eg 0.7 ms / cm). The reducing soil conductivity may be reducing the soil conductivity from 2.7 ms / cm to below 0.8 ms / cm (eg 0.7 ms / cm). The reducing the soil exchangeable sodium ion content may be reducing the soil exchangeable sodium ion content by 66%.

[0040] The reduction was 66% compared to the control method (CK3).

[0041] The reducing the soil exchangeable sodium ion content may be reducing the soil exchangeable sodium ion content to below 3233 mg / kg (eg 3232 mg / kg).

[0042] The reducing the soil exchangeable sodium ion content may be reducing the soil exchangeable sodium ion content from 9636 mg / kg to below 3233 mg / kg (eg 3232 mg / kg).

[0043] Reducing the exchangeable potassium ion content in the soil can reduce the exchangeable potassium ion content in the soil by 40%.

[0044] The 40% reduction was compared to the control method (CK3).

[0045] Reducing the exchangeable potassium ion content in the soil may be reducing the exchangeable potassium ion content in the soil to below 100 mg / kg.

[0046] Reducing the exchangeable potassium ion content in the soil may be reducing the exchangeable potassium ion content in the soil from 166 mg / kg to below 100 mg / kg.

[0047] Reducing the available phosphorus content in the soil can reduce the available phosphorus content in the soil by 65%.

[0048] The reduction of 65% was compared with the control method (CK3).

[0049] Reducing the available phosphorus content in the soil may be reducing the available phosphorus content in the soil to below 21 mg / kg (eg, 20 mg / kg).

[0050] Reducing the available phosphorus content in the soil may be reducing the available phosphorus content in the soil from 58 mg / kg to below 21 mg / kg (eg, 20 mg / kg).

[0051] Increasing the exchangeable calcium ion content of soil can increase the exchangeable calcium ion content of soil by 18%.

[0052] The improvement of 18% is compared with the control method (CK3).

[0053] Increasing the exchangeable calcium ion content of the soil may be increasing the exchangeable calcium ion content of the soil to above 3570 mg / kg.

[0054] Increasing the exchangeable calcium ion content of the soil may be to increase the exchangeable calcium ion content of the soil from 3033 mg / kg to above 3570 mg / kg.

[0055] Improving soil organic matter content can increase soil organic matter content by 33%.

[0056] The improvement of 33% is compared with the control method (CK3).

[0057] Increasing the soil organic matter content may be increasing the soil organic matter content to above 11 g / kg.

[0058] Increasing the soil organic matter content may be to increase the soil organic matter content from 9 g / kg to above 11 g / kg.

[0059] Increasing the soil alkaline nitrogen content can increase the soil alkaline nitrogen content by 349%.

[0060] The improvement of 349% was compared with the control method (CK3).

[0061] Increasing the soil alkaline nitrogen content may be increasing the soil alkaline nitrogen content to above 188 mg / kg.

[0062] Increasing the soil alkaline nitrogen content may be increasing the soil alkaline nitrogen content from 42 mg / kg to above 188 mg / kg.

[0063] Increasing the available potassium content in soil can increase the available potassium content in soil by 146%.

[0064] The improvement of 146% was compared with the control method (CK3).

[0065] Increasing the available potassium content in the soil may be to increase the available potassium content in the soil to above 294 mg / kg.

[0066] Increasing the available potassium content in the soil may increase the available potassium content in the soil from 119 mg / kg to above 294 mg / kg.

[0067] Compared with the method for improving saline-alkali soil and restoring vegetation of the present invention, the control method does not perform the treatment of step (2), nor does it perform the treatment of applying organic fertilizer and amino acid water-soluble fertilizer in step (1), and the other operations are the same.

[0068] In the method, the survival rate of the Puccinellia tenuifolia plants after transplanting is more than 95%.

[0069] The salt-alkali spots are salt-alkali spots on grasslands.

[0070] The present invention also protects the application of any of the above methods in grassland management.

[0071] Specifically, any of the above-mentioned soils is surface soil.

[0072] Topsoil: soil at a depth of 0-20 cm from ground level.

[0073] Specifically, the conductivity of the saline-alkali soil before improvement is 0.8ms / cm-4ms / cm and the pH value is 9-11.

[0074] Specifically, any of the above organic fertilizers is decomposed sheep dung or decomposed cow dung.

[0075] Specifically, any of the above-mentioned Puccinellia tenuifolia is Puccinellia serrata.

[0076] The grassland may be a degraded grassland.

[0077] The grassland may be a lowland meadow.

[0078] The grassland may be a grassland in Inner Mongolia.

[0079] The grassland may be the grassland in Hulunbuir, Inner Mongolia.

[0080] The grassland can be Chenbaerhu Banner, Hulunbuir City, Inner Mongolia The grassland may be the grassland of Huhedaobugacha, Chenbaerhu Banner, Hulunbuir City, Inner Mongolia.

[0081] The grassland may be a grassland with an annual precipitation of 200-300 mm.

[0082] The method provided by the present invention is used to transplant Puccinellia tenuifolia on saline-alkali land, which can improve the transplant survival rate of Puccinellia tenuifolia on the one hand, and improve the soil physical and chemical properties of the saline-alkali land on the other hand (reduce the salt content of the soil, effectively control the return of soil to salinity and alkali, and improve soil fertility). The present invention can quickly restore vegetation on saline-alkali land, improve the productivity of grasslands where saline-alkali lands are located, and improve the ecological environment of grasslands. DETAILED DESCRIPTION

[0083] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0084] The experimental methods in the following embodiments, unless otherwise specified, are all conventional methods, and are carried out in accordance with the techniques or conditions described in the literature in the field or in accordance with the product instructions. The materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be obtained from commercial channels. Unless otherwise specified, the quantitative tests in the following embodiments are all repeated three times, and the results are averaged. Surface soil: soil at a depth of 0-20 cm from the ground level. Seed purity refers to the percentage of the weight of normal seeds in a certain amount of seeds to the total weight (including impurities other than normal seeds). The model of the seedling pot used in the embodiment: diameter 8-10 cm, height 8-10 cm. The alkaligrana seeds used in the embodiment are alkaligrana sempervirens ( Puccinellia hauptian ) seeds. The quality control standard of Puccinellia tenuifolia seeds is "germination rate of more than 75% and purity of more than 90%". The organic fertilizer used in the example is decomposed sheep manure. Culture medium: loam obtained from farmland in Hailar District, Inner Mongolia (pH value is 6.28, conductivity is 0.43ms / cm). The numerical values ​​in the examples are in the form of mean ± standard error. Microsoft Excel 2021 software was used to perform statistics on the data. Different lowercase letters in the same column indicate significant differences between different treatments ( P <0.05).

[0085] Salt-alkali patch: a patchy area in the grassland where no grass grows, with a surface soil conductivity of 0.8ms / cm-4ms / cm and a pH of 9-11. Causes of salt-alkali patch: Natural grasslands are usually low-lying wetland meadows. As evaporation is greater than precipitation, groundwater moves up and down, and soil heterogeneity leads to water and salt redistribution, forming new patches of soluble salts on the soil surface. The salt (alkali) content in the soil in the patches increases to a level that is sufficient to hinder the normal growth of forage, resulting in reduced forage biological production and reduced grassland utilization performance.

[0086] Soil sampling method: In the unplanted area, take the surface soil from the three vertices of an equilateral triangle with a side length of 60 cm and mix them to obtain one soil sample.

[0087] Example The test site is Huhedaobugacha, Chenbaerhu Banner, Hulunbuir City, Inner Mongolia (119°52´E, 49°36´N); the average annual temperature is 0℃, and the annual precipitation is 200-300mm; the grassland vegetation type is low-lying wetland meadow (the grassland is used for mowing), and the area of ​​saline-alkali spots accounts for about 10% of the grassland area.

[0088] 1. Seedling cultivation 1. Breaking dormancy The seeds of Puccinellia tenuifolia were soaked in water at room temperature for 3 h and then dried (dried at room temperature for 24 h).

[0089] 2. Preparation before sowing Take the seedling pot, fill it with culture medium, and then water it thoroughly.

[0090] 3. Planting seeds Sow seeds after the following indicators are met for 5 consecutive days: average daily temperature ≥ 8℃ and minimum temperature ≥ 5℃.

[0091] Sow the seeds from step 1 in the seedling pots from step 2 (8-10 seeds per seedling pot), and then cover the seeds with a culture matrix (no thicker than 0.8 cm). Cultivate and manage outdoors normally (water during drought, no more than once a day, and remove weeds manually). Stop watering 3-5 days before transplanting, and water thoroughly before transplanting.

[0092] 2. Carry out land improvement Test plot: 18 saline-alkali patches in the test site. One soil sample was taken from each saline-alkali patch for soil physical and chemical property analysis. Indicators for soil physical and chemical property analysis: exchangeable cations (calcium, magnesium, potassium, sodium) content, pH value, electrical conductivity, organic matter content, alkaline nitrogen content, available phosphorus content, and available potassium content. Randomly grouped, 3 saline-alkali patches as 1 group (3 replicates), a total of 6 groups. The data of each index of each group are shown in Tables 1 and 2. There were no significant differences in the data of various indexes of the 6 groups of saline-alkali patches.

[0093] Table 1

[0094] Table 2

[0095] Group 1 was designated as treatment I, group 2 as treatment II, and group 3 as treatment III. Group 4 was designated as CK1, group 5 as CK2, and group 6 as CK3.

[0096] Treatment I, II, III and CK1: On June 28, 2024, organic fertilizer (3 kg / m 2 ), then deep plowing (depth of 30cm), and then harrowing (to make the soil fine and flat). On July 5, 2024, drip irrigation of amino acid water-soluble fertilizer solution (in terms of amino acid water-soluble fertilizer, the application amount is 0.02L / m 2 ).

[0097] CK2 operation: On June 28, 2024, organic fertilizer (3 kg / m 2 ), then deep plowing (depth of 30cm), and then raking (to make the soil fine and flat).

[0098] Operation of CK3: On June 28, 2024, deep plowing (depth of 30 cm) was carried out in the experimental plot, and then raking (to make the soil fine and flat).

[0099] Amino acid water-soluble fertilizer solution is obtained by diluting amino acid water-soluble fertilizer with water to 500 times the volume. Amino acid water-soluble fertilizer (liquid preparation): the seller is Tianjin Yuchuan Jinhong Environmental Protection Technology Co., Ltd.; the manufacturer is Tianjin New Age Agricultural Technology Co., Ltd., the product name is "Antifreeze Foliar Fertilizer (Containing Amino Acid Water-soluble Fertilizer)"; registration certificate number: Agricultural Fertilizer (2017) No. 6147; main technical indicators: amino acid ≥ 100g / L, calcium ≥ 30g / L.

[0100] 3. Transplanting Operation of treatment I, treatment II and treatment III: On July 5, 2024, use a pointed ox heart to dig holes in the experimental plots where step 2 was completed (the soil dug out is saline-alkali soil), transplant the seedlings (at this time, the seedling height ≥ 10 cm) 30 days after sowing in step 1 into the holes (the plant spacing and row spacing are both 30 cm), and then cover with saline-alkali soil, and then water thoroughly. Normal cultivation management (water in time according to soil moisture conditions within 30 days). When the seedlings are 30 cm tall, they can be mowed appropriately, leaving a stubble height of 10 cm. Treatment I, treatment II and treatment III use different hole depths (hole depth refers to the distance between the bottom of the hole and the ground plane) and covering distances (covering distance refers to the distance between the soil plane and the ground plane after covering, and the soil plane is lower than the ground plane), see Table 3. 150 seedlings were transplanted in each saline-alkali spot.

[0101] Operations for CK1, CK2, and CK3: No operation (i.e., no transplanting).

[0102] Table 3

[0103] 4. Statistical survival rate After 45 days of transplanting, the number of surviving plants in each experimental plot was counted, and the survival rate of each group was further counted. The results are shown in Table 4. The hole depth and the distance of covering soil affect the survival rate of Puccinellia sinensis after transplanting. In the order of survival rate from high to low, treatment II>treatment III>treatment I. Therefore, the transplanting effect is best when the hole depth is 15 cm and the distance from the ground level after covering soil is 3 cm.

[0104] Table 4

[0105] 5. Testing the physical and chemical properties of soil 45 days after transplanting, one soil sample was taken from each saline-alkali spot for soil physical and chemical property analysis. Indicators for soil physical and chemical property analysis: exchangeable cation (calcium, magnesium, potassium, sodium) content, pH value, conductivity, organic matter content, alkaline nitrogen content, available phosphorus content, and available potassium content. The data of each index of each group are shown in Tables 5 and 6.

[0106] The results are shown in Tables 5 and 6.

[0107] Table 5

[0108] Table 6

[0109] Compared with CK3 and CK2, CK1 significantly reduced the exchangeable sodium ion content in saline-alkali soil ( P <0.05, significantly reduced the pH value and electrical conductivity of saline-alkali soil ( P <0.05, significantly increased the content of alkaline nitrogen in saline-alkali soil ( P <0.05), indicating that amino acid water-soluble fertilizer can improve saline-alkali soil to a certain extent.

[0110] Compared with the three controls (CK1, CK2, and CK3), the three treatments (treatment I, treatment II, and treatment III) significantly reduced the contents of exchangeable potassium and exchangeable sodium ions in saline-alkali soil ( P <0.05); the contents of exchangeable potassium ions and exchangeable sodium ions in the saline-alkali soil of treatment II were significantly lower than those in treatments I and III ( P <0.05). Compared with the three controls (CK1, CK2, and CK3), treatment II significantly increased the exchangeable calcium ion content in saline-alkali soil ( P <0.05). Compared with the three controls (CK1, CK2, and CK3), the three treatments (treatment Ⅰ, treatment Ⅱ, and treatment Ⅲ) significantly reduced the pH value and electrical conductivity of the saline-alkali soil; the pH value of the saline-alkali soil in treatment Ⅱ was significantly lower than that in treatments Ⅰ and Ⅲ ( P <0.05). Compared with the three controls (CK1, CK2, and CK3), treatments II and III significantly increased the organic matter content, alkaline nitrogen content, and available potassium content of saline-alkali soil ( P <0.05); the organic matter content of the soil in treatment II was significantly higher than that in treatment III ( P <0.05).

[0111] Note: The testing methods for various indicators of soil physical and chemical properties analysis are as follows: Detection of exchangeable cation (potassium, sodium) content: LY / T1246-1999 "Determination of exchangeable potassium and sodium in forest soils".

[0112] Detection of exchangeable cation (calcium, magnesium) content: NY / T 1121.13-2006 "Soil Testing Part 13: Determination of Exchangeable Calcium and Magnesium in Soil".

[0113] Detection pH value: NY / T 1121.2-2006 "Soil Testing Part 2: Determination of Soil pH".

[0114] Conductivity detection: HJ 802-2016 "Electrode method for determination of soil conductivity".

[0115] Detection of organic matter content: NY / T 1121.6—2006 "Soil Testing Part 6: Determination of Soil Organic Matter".

[0116] Detection of alkaline nitrogen content: F-HZ-DZ-TR-0051 (FHZDZTR0051) "Determination of hydrolyzable nitrogen in soil by alkaline diffusion method".

[0117] Detection of available phosphorus content: NY / T 1121.7-2014 "Soil Testing Part 7: Determination of Available Phosphorus in Soil".

[0118] Detection of available potassium content: NY / T 889-2004 "Determination of available potassium and slow-release potassium content in soil".

[0119] The present invention has been described in detail above. It will be apparent to those skilled in the art that the present invention may be implemented in a wide range under equivalent parameters, concentrations and conditions without departing from the spirit and scope of the present invention and without the need for unnecessary experimentation. Although the present invention provides specific embodiments, it should be understood that further improvements may be made to the present invention. In short, according to the principles of the present invention, this application intends to include any changes, uses or improvements to the present invention, including changes made by conventional techniques known in the art that depart from the scope disclosed in this application. Applications of some of the basic features may be made within the scope of the following appended claims.

Claims

1. A method for soil improvement and vegetation reconstruction on saline-alkali spots, comprising the following steps: (1) Apply organic fertilizer to the saline-alkali soil to be improved, then till the soil, and then apply amino acid water-soluble fertilizer; (2) After completing step (1), dig a hole, transplant the alkali grass plants into the hole, and then cover the hole with soil, so that the vegetation is restored through the growth of the alkali grass and the saline-alkali soil is improved; the depth of the hole is 15 cm, and after covering the hole with soil, the soil level is lower than the ground level and the distance between the soil level and the ground level is 3 cm.

2. The method according to claim 1, characterized in that: The amount of amino acid water-soluble fertilizer applied is 0.02L / m 2 .

3. The method according to claim 1 or 2, characterized in that: The amount of organic fertilizer applied is 3kg / m 2 .

4. The method according to any one of claims 1 to 3, characterized in that: The Puccinellia repens plants used for transplanting are Puccinellia repens plants in the seedling stage.

5. The method according to any one of claims 1 to 4, characterized in that: The Puccinellia repens plants used for transplanting are those with a plant height ≥10 cm.

6. The method according to any one of claims 1 to 5, characterized in that: The preparation method of the Puccinellia tenuifolia plants for transplanting comprises the following steps: taking Puccinellia tenuifolia seeds, breaking the dormancy of the seeds, sowing the seeds in a seedling pot filled with a culture matrix, and then covering the seeds with the culture matrix.

7. The method according to any one of claims 1 to 6, characterized in that: The improvement is embodied in at least one of the following (a) to (i): (a) Lowering soil pH; (b) reduce soil electrical conductivity; (c) reduce the exchangeable sodium ion content of soil; (d) reduce the exchangeable potassium ion content in the soil; (e) Reducing available phosphorus content in soil; (f) Increase the exchangeable calcium ion content in the soil; (g) increasing soil organic matter content; (h) increasing the soil alkaline nitrogen content; (i) Increase the available potassium content in the soil.

8. The method according to any one of claims 1 to 7, characterized in that: The survival rate of Puccinellia tenuifolia plants after transplanting was over 95%.

9. The method according to any one of claims 1 to 8, characterized in that: The salt-alkali spots are salt-alkali spots on grasslands.

10. Use of the method according to any one of claims 1 to 9 in grassland management.

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