Artificial propagation method of Sichuan parabramis pekinensis

Abstract

The invention discloses an artificial breeding method of Sichuan parabramis pekinensis. The method specifically comprises the following steps: performing flushing stimulation on a Sichuan parabramis pekinensis parent one month before breeding; the water temperature of the spawning pond is set to be stabilized at 25 DEG C or above, and the spawning pond is covered with a dense mesh; selecting a female parent with an obvious ovary outline and a soft and elastic abdomen as a female parent, and selecting a male parent with milk white thick semen flowing out by slightly pressing the abdomen as a male parent; the selected parents are subjected to spawning induction medicine injection, and domperidone DOM and luteinizing hormone LRH-A2 are selected as the spawning induction medicine; respectively catching male and female parents when the parents are induced to oestrus peak, collecting eggs and seminal fluid, mixing, and adding water for fertilization; after waist points (swim bladders) are formed, fry rearing is conducted. The breeding method provided by the invention can significantly improve the breeding difficulty of Sichuan parabramis pekinensis and improve the breeding efficiency, and has an application prospect in the field of breeding of Sichuan parabramis pekinensis.

Description

Technical Field

[0001] The invention relates to the technical field of fish breeding methods, and in particular to an artificial breeding method for Sichuan bream. Background Art

[0002] Sichuan bream (Sinibrama taeniatus) ) belongs to the order Cypriniformes ( Cypriniformes ) Cyprinidae Cyprinidae ) Subfamily (Cooking) The genus Sinensis has obvious black lines vertically on its body, also known as ink line fish. Sichuan Sinensis is a small fish unique to the upper reaches of the Yangtze River in my country. It is mainly distributed in the main and tributary waters of the upper reaches of the Yangtze River, including the Minjiang River, Qingyi River and Dadu River. It lives in the middle and upper layers of the water body and often becomes a dominant population in some water bodies. In recent years, with the destruction of the ecological environment of the main and tributary rivers of the upper reaches of the Yangtze River, as well as the increase in fishing intensity and the use of illegal fishing tools, the living space of Sichuan Sinensis has been compressed and the population has continued to decline sharply. At the same time, there is a very small amount of artificial breeding of Sichuan Sinensis in Sichuan, and the distribution is uneven, the scale is small, and the artificial breeding technology is imperfect.

[0003] Based on this, the present application intends to develop an artificial breeding method for Sichuan bream, in order to provide a technical reference for significantly improving the breeding difficulties of Sichuan bream and improving the breeding efficiency. Summary of the invention

[0004] The invention aims to provide an artificial propagation method of Sichuan bream, which can significantly improve the propagation difficulty of Sichuan bream and improve the propagation efficiency, and has application prospects in the field of Sichuan bream breeding.

[0005] In order to achieve the above object, the present invention provides an artificial propagation method of Sichuan bream, comprising the following steps: Parent pretreatment: One month before breeding, the Sichuan bream parents were stimulated by flushing water; Preparation of spawning pool: Set the water temperature of the spawning pool to be stable above 25°C and cover the spawning pool with a dense mesh; Parent selection and transfer: Select the female parent with obvious ovary outline and soft and elastic abdomen, and select the male parent with milky white sticky semen flowing out when the abdomen is lightly pressed; and transfer the parents to the spawning pool; Induction of labor: Injection of labor-inducing drugs such as DOM and luteinizing hormone-releasing hormone LHRH-A2 is performed on the selected parents; Artificial insemination: When the parents are induced to estrus, the male and female parents are captured separately, the eggs and semen are collected, mixed and added to water for fertilization; Hatching: The eggs will shed their membranes after 48 hours, and the waist point will be formed after 4 days, and the fish can be put into the pond for fry cultivation.

[0006] Preferably, the flushing stimulation is performed once a week after mid-April, each time for 2 hours; from late April to early May, flush once a day, each time for 1 to 2 hours.

[0007] Preferably, the male to female ratio of the above male parent is 1:1.2.

[0008] Preferably, the dosage of the above-mentioned oxytocin drug is: 5-6 mg DOM + 10-12 μg LRH-A2.

[0009] The present invention has the following advantages: The present application provides a novel breeding process for Sichuan bream, which provides a set of solutions for artificial breeding of Sichuan bream, and can become a preferred species for large-scale pond breeding and release. This method causes little harm to fish and has the characteristics of high egg laying rate, spawning rate and fertilization rate.

[0010] The artificial breeding method provided by the present invention has strong operability, and the DOM and LRH-A2 are scientifically matched and mixed for induced spawning, which has little harm to fish, and has high egg laying rate and egg rate. In addition, the artificial breeding operation is simple, and no large or expensive equipment is required. The method is relatively effective and can successfully complete the fertilization process.

[0011] The artificial propagation method of Sichuan bream provided by the invention has strong scientific guidance. Under the method, Sichuan bream has a large egg-laying amount and a high egg-laying rate, and the survival rate of seedlings also reaches a high level. Therefore, the invention has certain guiding significance whether it is used for breeding production or scientific research and propagation of Sichuan bream germplasm protection. DETAILED DESCRIPTION

[0012] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0013] Note: The experimental methods in the following examples are conventional methods unless otherwise specified, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples are all commercially available unless otherwise specified.

[0014] Example 1 This embodiment provides an artificial propagation method for Sichuan bream, which is as follows: Water flushing stimulation for parents: One month before parent breeding, that is, after mid-April, the Sichuan bream in the pond should be flushed with water once a week for 2 hours each time; from late April to early May before inducing spawning, flush it once a day for 1 to 2 hours each time.

[0015] Preparation of spawning pond: Prepare the spawning pond in early to mid-May. The spawning pond is a circular spawning pond with a diameter of 8 meters and a depth of 1.2-1.5 meters. The water temperature is stable at above 25°C. A small net cage is set in the spawning pond. In order to prevent the broodstock from jumping out and causing unnecessary casualties, the spawning pond must be covered with a dense mesh.

[0016] Parent selection: Sichuan bream with obvious ovary outline and soft and elastic abdomen are selected as female parents. Male Sichuan bream with milky white sticky semen flowing out when the abdomen is lightly pressed are selected as male parents; the male-female ratio is generally 1:1.2, and each batch of parents is about 10 groups.

[0017] Parent transfer: Because Sichuan bream are good at jumping, have violent temperaments, and are prone to hypoxia, we choose to use a dragnet to transport the parents from the pond to the spawning pond. The dragnet operation should be light and the transportation tool is a 80cm long soft cloth clip. The cloth clip can transport one parent fish at a time to avoid scratching each other. The transported parents are weighed first and then put into the net cage.

[0018] Induced labor: Put the parent in a cloth clip and put it in the water to inject the inducing drug. The inducing personnel are required to coordinate their movements and try to shorten the time that the Sichuan bream is out of water. The inducing drug and dosage are: 5-6mg DOM + 10-12μg LRH-A2; the injection dose of male fish is 70% of that of female fish. The two-needle injection method is adopted, and both are injected into the pectoral fins. The first injection injects 1 / 3, the needle interval is 10-12 hours, and the second injection injects the remaining 2 / 3. The needle is inserted at a 45° angle, and the drug is slowly injected at a depth of 1mm. The injection time is determined according to the water temperature. Among them, DOM is dioic acid; LHRH-A2 is luteinizing hormone releasing hormone.

[0019] Artificial insemination: It takes about 8 hours for the broodstock to enter estrus. When the broodstock begins to enter estrus and reaches its peak, catch the male and female broodstock separately, wipe the water off the fish body with a soft towel, then squeeze the eggs and semen into a dry basin, and stir them continuously with hard chicken feathers to make them fully mixed, then add clean water and stir to fertilize.

[0020] Hatching: Fish eggs usually shed their membranes after 48 hours, and the waist point (swim bladder appears) is formed after 4 days, and can be put into the pond for fry cultivation.

[0021] During the hatching of fry, some fertilized eggs were selected to observe the embryonic development under a dissecting microscope. The observations were made every 0.5 h during the cell division stage and once every 1.5 to 3.0 h after the blastocyst stage.

[0022] By statistically calculating the survival rate, induced fertilization rate, fertilization rate and spawning rate of Sichuan bream after reproduction using the above method, it was found that the survival rate was 80-85%, the induced fertilization rate was 70-75%, the fertilization rate was 40-50%, and the spawning rate was 70-80%.

[0023] It can be seen that the artificial breeding method provided by the present invention can significantly improve the breeding efficiency of Sichuan bream, can be used as a standard for the artificial breeding process of Sichuan bream, provides certain guidance for the artificial breeding of Sichuan bream and its germplasm protection, and has potential application value in the field of Sichuan bream breeding.

[0024] Although the content of the present invention has been described in detail through the above preferred embodiments, it should be appreciated that the above description should not be considered as a limitation of the present invention. After reading the above content, it will be apparent to those skilled in the art that various modifications and substitutions of the present invention will occur. Therefore, the protection scope of the present invention should be limited by the appended claims.

Claims

1. An artificial propagation method for Sichuan bream, characterized in that: Contains the following: Parent pretreatment: One month before breeding, the Sichuan bream parents were stimulated by flushing water; Preparation of spawning pool: Set the water temperature of the spawning pool to be stable above 25°C and cover the spawning pool with a dense mesh; Parent selection and transfer: Select the female parent with obvious ovary outline and soft and elastic abdomen, and select the male parent with milky white sticky semen flowing out when the abdomen is lightly pressed; and transfer the parents to the spawning pool; Induction of labor: Injection of labor-inducing drugs, including DOM and LRH-A2, is performed on the selected parents; Artificial insemination: When the parents are induced to estrus, the male and female parents are captured separately, the eggs and semen are collected, mixed and added to water for fertilization; Hatching: The eggs will shed their membranes after 48 hours, and the waist point will be formed after 4 days, and the fish can be put into the pond for fry cultivation.

2. The artificial propagation method according to claim 1, characterized in that: The flushing stimulation is once a week after mid-April, each time for 2 hours; from late April to early May, flush once a day, each time for 1 to 2 hours.

3. The artificial propagation method according to claim 1, characterized in that: The male parent has a male to female ratio of 1:1.

2.

4. The artificial propagation method according to claim 1, characterized in that: The dosage of the oxytocin drug is: 5-6 mg DOM + 10-12 μg LRH-A2.