A bactericidal composition and its use

Abstract

The present application relates to a kind of fungicide composition and its application, including active ingredient A and active ingredient B, the active ingredient A is the compound shown in formula (I), and active ingredient B is any one of bromine fungicide, prochloraz.The mass ratio of the active ingredient A and active ingredient B is 1:35~34:1.The fungicide composition of the present application or its preparation can improve the control effect, has significant synergistic effect, and can effectively reduce the amount of drug, delay the development of disease resistance.

Description

Technical Field

[0001] This invention relates to the field of pesticides and fungicides, specifically to a fungicidal composition and its application. Background Technology

[0002] Methoxyacrylate fungicides are a class of low-toxicity, broad-spectrum, highly effective, systemic fungicides that exhibit good activity against almost all fungal diseases (oomycetes, phycophytes, ascomycetes, and deuteromycetes), such as powdery mildew, anthracnose, rust, net blotch, downy mildew, and rice blast. Compound (I) is a methoxyacrylate fungicide developed by Sumitomo Chemicals Co., Ltd., CAS Registry Number: 2454319-63-0, with the following specific chemical structure:

[0003]

[0004] Chemical control remains the most effective means of controlling plant diseases. However, long-term use of a single agent can easily lead to a significant increase in disease resistance, resulting in a substantial increase in pesticide application and consequently, more severe environmental pollution. Combining two active ingredients without cross-resistance can significantly delay the development of disease resistance, broaden the fungicidal spectrum, and improve efficacy, thereby reducing pesticide usage and environmental pollution. This invention, by mixing compound (I) with bromodiphenyl ether and prochloraz, surprisingly discovered that this composition has a significant synergistic effect, significantly improving the therapeutic and protective effects, reducing pesticide dosage, broadening the control spectrum, lowering control costs, and ensuring high crop safety. Summary of the Invention

[0005] Based on the above, the present invention aims to provide a fungicidal composition, mainly used for the prevention and control of plant fungal diseases. This composition or its formulation can enhance efficacy, reduce dosage, prolong the duration of effectiveness, and delay the development of resistance.

[0006] To achieve the above objectives, the following technical solution is provided: a bactericidal composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula: Active ingredient B is either bromochloronitrofurazone or imazalil;

[0007] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:35 to 34:1 or any value between the above values;

[0008] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:32 to 28:1;

[0009] When the active ingredient B is bromodiphenyl nitrile, the mass ratio of the compound of formula (I) to bromodiphenyl nitrile is 1:28 to 28:1;

[0010] Furthermore, the mass ratio of the compound of formula (I) to bromobenzonitrile is 1:28 to 14:1.

[0011] Furthermore, the mass ratio of the compound of formula (I) to bromodiphenyl ether is 1:28, 1:14, 1:5, 2:5, 5:1, or 14:1;

[0012] When the active ingredient B is imazalil, the mass ratio of the compound of formula (I) to imazalil is 1:32 to 26:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to imazalil is 1:32 to 13:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to imazalil is 1:32, 1:13, 1:6, 2:3, 6:1, or 13:1;

[0015] Furthermore, based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 0.5 to 90 wt%.

[0016] Furthermore, based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 80 wt%.

[0017] Furthermore, the bactericidal composition, in addition to containing the active ingredient, also contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

[0018] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0019] Further, the dispersant is selected from one or more of the following: lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0020] Further, the emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0021] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0022] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0023] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0024] Furthermore, the defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0025] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0026] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0027] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0028] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0029] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0030] Furthermore, the dosage form of the bactericidal composition is selected from solid dosage forms and / or liquid dosage forms and / or seed treatment formulations;

[0031] The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0032] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0033] Furthermore, the bactericidal composition can be prepared into a pesticide-acceptable formulation, wherein the formulation is a microemulsion, water-in-oil emulsion, suspension, dispersible oil suspension, soluble concentrate, emulsifiable concentrate, suspension emulsion, microcapsule suspension, water-dispersible granules, wettable powder, granules, seed treatment suspension, or seed treatment dry powder.

[0034] Furthermore, the formulation is a microemulsion, emulsifiable concentrate, suspension concentrate, water emulsion, water-dispersible granules, wettable powder, or seed treatment suspension.

[0035] The present invention also discloses the application of the bactericidal composition described above in the prevention and control of plant diseases;

[0036] Furthermore, the plant diseases mentioned are plant diseases caused by pathogens such as *Blumiria*, *Erysiphe*, *Gibberella*, *Sclerotinia*, *Botryotinia*, *Puccinia*, *Tilletia*, *Gymnosporzngium*, *Phakopsora*, *Ustilago*, *Rhizoctonia*, *Verticillium*, *Alternaria*, *Pyriculara*, *Botrytis*, *Cercospora*, *Fusarium*, *Colletotrichum*, *Rhizoctonia*, and *Sclerotium*.

[0037] Furthermore, the plant disease is a plant disease caused by fungi of the genus *Anthracnose*.

[0038] Furthermore, the aforementioned anthrax fungi include *Colletotrichum higginsianum*, *Colletotrichum orbiculare*, *Colletotrichum lindemuthianum*, *Colletotrichum capsici*, and *Colletotrichum gloeosporioides*.

[0039] Furthermore, the plant disease caused by the anthracnose fungus is cucumber anthracnose [Colletotrichumorbiculare].

[0040] Anthracnose in cucumbers can occur during the seedling and mature stages, affecting the cucumber plants and their yield, thus causing economic losses to growers.

[0041] The present invention has the following beneficial effects:

[0042] 1) The bactericidal composition provided by the present invention has high bactericidal activity and has a synergistic effect on the target at a certain mass ratio;

[0043] 2) The bactericidal composition provided by this invention can significantly improve the quality and grade of crops;

[0044] 3) The bactericidal composition provided by the present invention is safe and efficient, and is safe for crops, non-target organisms, beneficial organisms and natural enemies. Detailed Implementation

[0045] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0046] Indoor toxicity testing:

[0047] Indoor bioassay for cucumber anthracnose

[0048] Test basis: NY / T 1156.2-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Mycelial Growth of Pathogenic Fungi"; NY / T 1156.6-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 6: Determination of Combined Effects of Mixtures".

[0049] Test pathogen: Cucumber anthracnose (isolated from anthracnose lesions exhibiting typical symptoms on cucumber fruits from Xiazhuang).

[0050] Test reagents: 95% bromochlorothalonil technical, 95% imazalil technical, 90% compound (I) technical, all of which were provided by the Group's R&D Center.

[0051] Reagent preparation: Dissolve the test drug in acetone first, then dilute with 0.1% Tween 80 aqueous solution. Prepare single-agent stock solutions separately, and set 5 series of mass concentrations according to the purpose of mixing and the drug activity.

[0052] Melt the PDA medium in a microwave oven and cool it to about 50°C. Following the principle of starting from low concentration and gradually increasing the concentration, take 1 mL of the prepared test solution and 9 mL of PDA medium and add them to a 9 cm diameter petri dish. Mix well to prepare a drug-containing plate of the corresponding concentration.

[0053] Inoculation: Under aseptic conditions, the activated pathogenic fungus was punched into a fungal cake using a 6mm diameter punch. After the drug-containing culture medium solidified, the fungal cake was placed in the center of the culture medium. Finally, the culture dish was sealed with sealing film and placed in an incubator at 27℃ and 70% relative humidity for cultivation. A blank control without the drug was set up. Each treatment was repeated 3 times.

[0054] Investigation: The growth of pathogenic fungal hyphae was investigated based on the growth of hyphae in blank control culture dishes. The diameter of colonies was measured with calipers in millimeters (mm). The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken.

[0055] Calculation method: Based on the survey results, the mycelial growth inhibition rate is calculated according to the following formula, in percentage (%), and the calculation result is rounded to two decimal places.

[0056] D = D1 - D2

[0057] In the formula:

[0058] D – Colony growth diameter;

[0059] D1—colony diameter;

[0060] D2 – Diameter of the mushroom cake.

[0061]

[0062] I – Mycelial growth inhibition rate;

[0063] D0—Correlation diameter of the blank control group;

[0064] D t — Diameter of colonies grown after chemical treatment.

[0065] Experimental statistics: Data were processed using probability value analysis. The DPS statistical analysis system was used to analyze the data and determine the toxicity regression line and EC50. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0066] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0067] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0068]

[0069] In the formula:

[0070] ATI – Actual Measured Toxicity Index of Mixtures;

[0071] S – EC of standard bactericides 50 The unit is milligrams per liter (mg / L);

[0072] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0073] TTI = TI A *P A +TI B *P B

[0074] In the formula:

[0075] TTI – Theoretical Toxicity Index of Mixtures;

[0076] TI A —A. Toxicity index of drug A;

[0077] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0078] TI B —Toxicity index of drug B;

[0079] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0080]

[0081] In the formula:

[0082] CTC – Cotoxicity Coefficient;

[0083] ATI – Actual Measured Toxicity Index of Mixtures;

[0084] TTI – Theoretical Toxicity Index of Mixtures.

[0085] Measurement results:

[0086] Table 1 shows the results of compound (I) controlling cucumber anthracnose EC. 50 The concentration of bromuconazole was 1.522 mg / L, which was effective in controlling cucumber anthracnose EC. 50 The concentration was 0.261 mg / L. Compound (I) exhibited additive or synergistic effects when mixed with bromodiphenyl ether at a mass ratio of 1:35 to 28:1. Specifically, at a mass ratio of 1:28 to 28:1, the co-toxicity coefficient was >120, demonstrating a synergistic effect. The co-toxicity coefficient of compound (I) with bromodiphenyl ether at a ratio of 2:5 was the highest, at 282.599, EC50. 50 The concentration was 0.121 mg / L, indicating a significant synergistic effect.

[0087] Table 1 shows the synergistic effect of different ratios of compound (I) with bromodiphenyl ether on cucumber anthracnose.

[0088]

[0089] Table 2 shows the results of compound (I) controlling cucumber anthracnose EC. 50 The concentration of prochloraz was 1.516 mg / L, which is effective in controlling cucumber anthracnose EC. 50 The concentration was 9.894 mg / L. Compound (I) exhibited additive or synergistic effects when mixed with imazalil in a mass ratio of 1:32 to 34:1. Specifically, when the mass ratio was 1:32 to 26:1, the co-toxicity coefficient was >120, demonstrating a synergistic effect. The co-toxicity coefficient of compound (I) with imazalil at a ratio of 2:3 was the highest, at 272.477, EC50. 50 The concentration was 1.131 mg / L, indicating a significant synergistic effect.

[0090] Table 2 shows the synergistic effect of different ratios of compound (I) with prochloraz on cucumber anthracnose.

[0091]

[0092]

[0093] Formulation Examples

[0094] Preparation Example 1:

[0095] 35% Formula (I) Compound·Bromoxynil Wettable Powder (10:25)

[0096] Formulation: 10% of compound (Ⅰ), 25% of bromodiphenyl ether, 5% of sodium polycarboxylate, 2% of α-olefin sulfonate, 5% of dispersant NNO, 6% of sodium lignosulfonate, 5% of sodium alkyl polyoxyethylene ether sulfonate, 5.5% of attapulgite, and the balance is made up with kaolin.

[0097] Preparation method: The active ingredients, other functional additives and fillers are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0098] Preparation Example 2:

[0099] 50% Formula (I) Compound·Iprodione Wettable Powder (18:32)

[0100] Formulation: 18% of compound (I), 32% of imazalil, 7% of alkylphenol polyoxyethylene ether methyl ether condensate sulfate, 5% of dispersant NNO, 5% of sodium alkyl polyoxyethylene ether sulfonate, 3.5% of sodium dodecyl sulfate, 5.5% of bentonite, 1.5% of silica, and the balance is made up with kaolin.

[0101] Preparation method: Same as in preparation example 1.

[0102] Preparation Example 3:

[0103] 28% Formula (I) compound·bromochloronitrile aqueous emulsion (8:20)

[0104] Formula: 8% of compound (Ⅰ), 20% of bromobenzoic acid, 12% of trimethylbenzene, 15% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium fatty alcohol polyoxyethylene ether sulfate, 5% of EO / PO block copolymer, 5% of glycerol, 1.5% of glycerin, 0.1% of silicone oil, 0.2% of xanthan gum, 0.1% of sodium benzoate, and deionized water to make up the balance;

[0105] Preparation method: According to the formulation ratio in the example, the active ingredient is dissolved in the solvent and an emulsifier is added to form a homogeneous oil phase. Deionized water and antifreeze are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the aqueous phase is added to the oil phase to form a well-dispersed water-emulsion formulation.

[0106] Preparation Example 4:

[0107] 25% Formula (I) Compound·Iprodione Water Emulsion (12:13)

[0108] Formula: 12% of compound (Ⅰ), 13% of imazalil, 11.5% of trimethylbenzene, 10% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium fatty alcohol polyoxyethylene ether sulfate, 5% of EO / PO block copolymer, 5% of ethylene glycol, 1.2% of glycerin, 0.1% of silicone oil, 0.2% of xanthan gum, 0.1% of sodium benzoate, and deionized water to make up the balance;

[0109] Preparation method: Same as in preparation example 3.

[0110] Preparation Example 5:

[0111] 20% Formula (I) compound·bromodimethalin emulsifiable concentrate (8:12)

[0112] Preparation formula: 8% of compound (Ⅰ), 12% of bromodiphenyl ether, 1% of BHT, 10% of propylene carbonate, 15% of DMF, 4.5% of cyclohexanone, 4% of agricultural emulsion 700#, 3.5% of calcium dodecylbenzenesulfonate, 1% of alkylphenol polyoxyethylene ether, 10% of glycerol fatty acid ester polyoxyethylene ether, and methyl oleate to make up the balance;

[0113] Preparation method: Add the active ingredients to the cosolvent according to the formulation ratio of the example, and add surfactants and other functional additives thereto. Stir and mix evenly in a stirring mixing tank to obtain emulsifiable oil.

[0114] Preparation Example 6:

[0115] 30% Formula (I) compound·Iprodione emulsifiable concentrate (12:18)

[0116] Preparation formula: 12% of compound (Ⅰ), 18% of imazalil, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of cyclohexanone, 3.5% of calcium dodecylbenzenesulfonate, 10% of glycerol fatty acid ester polyoxyethylene ether, and methyl oleate to make up the balance;

[0117] Preparation method: Same as in preparation example 5.

[0118] Field efficacy trials

[0119] Field efficacy trial of cucumber anthracnose

[0120] Experimental location: Vegetable greenhouses in Wangjiayu, Feicheng City, Tai'an City, Shandong Province;

[0121] The test crop was cucumber (Jinza No. 1); the water and fertilizer conditions and growth status were consistent, making it relatively susceptible to disease. The conditions met the requirements for field trials.

[0122] Experimental period: Planted on December 5, 2023, under protected cultivation, during the fruiting period, with excellent growth.

[0123] Test reagents and dosages:

[0124] Table 4. Comparison of pesticide dosage in field trials

[0125]

[0126]

[0127] Experimental treatments included a single-agent treatment and a water control. Two applications were administered 7 days apart, and each treatment was repeated four times. The plots were divided into 20m² areas. 2 Randomized block designation. Ensure application is protected from rain, strong winds, and extreme temperatures. The application equipment is a 3WBD-18L backpack electric sprayer with a spray volume of 675L / hm². 2 .

[0128] Investigation time and frequency: Disease surveys were conducted before the first application, 7 days after the first application, and 7 days after the last application, for a total of 3 surveys. The number of diseased leaves and the relative severity were recorded.

[0129] Survey method: Five points were selected from the diagonal of each area. Three plants were marked at each point. Ten leaves were checked from bottom to top on each plant. A total of 15 plants and 150 leaves were checked.

[0130] The grading method refers to "GB / T 17980.112-2004 Field Efficacy Test Guidelines for Pesticides (II) Fungicides for the Control of Anthracnose in Cucurbits", and the specific grading is as follows:

[0131] There are 9 levels in total:

[0132] Grade 0: No lesions;

[0133] Grade 1: The lesion area accounts for less than 5% of the total leaf area;

[0134] Grade 3: The lesion area accounts for 6% to 10% of the total leaf area;

[0135] Level 5: The lesion area accounts for 11% to 25% of the total leaf area;

[0136] Level 7: Lesions cover 26% to 50% of the total leaf area;

[0137] Level 9: The lesion area accounts for more than 51% of the total leaf area;

[0138] Methods for calculating disease severity index and drug efficacy:

[0139]

[0140] During the experiment, cucumbers in all treatment plots grew well, and none of the pesticides at the tested concentrations caused phytotoxicity to cucumber plants or other non-target organisms. The experimental data were analyzed using Duncan's New Multiple Range (DMRT) biostatistical method with DPS software to evaluate the significance of differences.

[0141] Results of field efficacy trials:

[0142] The results showed that 35% of compound (I)·bromodiflubenzuron wettable powder (10:25) and 25% of compound (I)·prochloraz emulsion (12:13) had good control effects on cucumber anthracnose. Seven days after the first application, their control efficacy was 76.35% and 74.29%, respectively. The difference between the mixed formulation and other single agents was significant.

[0143] Table 5. Results of field efficacy trials (7 days after the first application)

[0144]

[0145] Note: Data in the table are the average of four replicates; lowercase letters indicate that the differences between treatments are statistically significant (p < 0.05).

[0146] The results showed that 35% of compound (I)·bromodiflubenzuron wettable powder (10:25) and 25% of compound (I)·prochloraz emulsion (12:13) had good control effects on cucumber anthracnose. Seven days after the last application, their control efficacy was 83.33% and 82.57%, respectively. The difference between the mixed formulation and other single agents was significant.

[0147] Table 6. Results of field efficacy trials (7 days after the last application)

[0148]

[0149] Note: Data in the table are the average of four replicates; lowercase letters indicate that the differences between treatments are statistically significant (p < 0.05).

[0150] No phytotoxicity or impact on crop growth and development was recorded during the experiment, indicating that the tested pesticide was safe for crops within the recommended dosage range. No effects on non-target organisms were found within the dosage range. No extreme weather events such as heavy rainfall or strong winds were recorded during the experiment.

[0151] Field efficacy trials showed that the compound shown in formula (I), combined with bromodiphenyl ether and imazalil, exhibited high control efficacy in cucumber anthracnose. It not only reduced the disease rate and disease index, demonstrating significant control effects, but also had little impact on yield. Furthermore, the mixed formulation showed a marked yield-increasing effect. Post-treatment observations indicated that cucumber growth in all treatment areas was normal, with no phytotoxicity observed, suggesting that each pesticide was safe for cucumber growth at the experimental dosage.

[0152] Through indoor toxicity testing and field trials, the compound shown in formula (I) of this invention, combined with either bromodiphenyl ether or prochloraz, exhibited excellent control efficacy against cucumber anthracnose. The fungicidal composition or formulation obtained by this invention demonstrates significant efficacy, exhibiting high efficiency, broad spectrum, low residue, long-lasting effect, and strong systemic properties. Furthermore, no phytotoxicity was observed in the experiments, indicating that the enhanced synergistic effect of the obtained fungicidal composition or formulation reduces production and usage costs while ensuring crop safety.

[0153] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A fungicidal composition characterized in that: The active ingredient A is a compound of formula (I) and the active ingredient B is bromothalonil. (I), the active ingredient B is bromothalonil, and the mass ratio of the compound of formula (I) to bromothalonil is 1:28 to 28:

1.

2. The bactericidal composition according to claim 1, characterized by, The mass ratio of the compound of formula (I) to bromothalonil is 1:28 to 14:

1.

3. The germicidal composition according to claim 2, characterized in that, The mass ratio of the compound of formula (I) to bromothalonil is 1:28, 1:14, 1:5, 2:5, 5:1 or 14:

1.

4. The germicidal composition according to claim 1, wherein The total content of the active ingredient A and the active ingredient B in the fungicidal composition is 0.5 to 90 wt% based on the total weight of the fungicidal composition.

5. The fungicidal composition according to claim 4, characterized in that, The total content of the active ingredient A and the active ingredient B in the fungicidal composition is 1 to 80 wt% based on the total weight of the fungicidal composition.

6. The germicidal composition according to claim 1, wherein The fungicidal composition further comprises an agriculturally acceptable auxiliary ingredient in addition to the active ingredients, and the auxiliary ingredient is selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

7. The germicidal composition according to claim 6, characterized in that, The fungicidal composition can be prepared into a formulation dosage form acceptable in the pesticide industry, and the formulation dosage form is microemulsion, emulsifiable concentrate, suspension, aqueous emulsion, water dispersible granule, wettable powder or seed treatment suspension.

8. The use of a fungicidal composition according to any one of claims 1 to 7 for controlling plant diseases, characterized in that, The plant disease is cucumber anthracnose caused by Colletotrichum spp.

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