A microbial inoculant and its application

Abstract

The present invention provides a microbial inoculant and its application, belonging to the field of biotechnology. The inoculant comprises the following components: Component A: Penicillium restrictum ( Penicillium restrictum ) and Penicillium verruculosum ( Penicillium verruculosum ); Component B: sodium alginate, fulvic acid, Panax japonicus Torr. extract, Polygonatum odoratum extract, corn starch; wherein, the addition amount of Penicillium restrictum is 1% - 3% of the total weight of the microbial inoculant, the addition amount of Penicillium verruculosum is 1% - 5% of the total weight of the microbial inoculant, and the balance is Component B. The microbial inoculant provided by the present invention can effectively prevent and control the soft rot of Chinese cabbage caused by Pectobacterium carotovorum ( Peclobacterium carolovorumsubsp.carolovorum ).

Description

Technical Field

[0001] The present invention belongs to the field of biotechnology, and specifically relates to a microbial inoculant and its application. Background Art

[0002] Chinese cabbage ( Brassica rapa ssp .pekinensis ) is a vegetable of the genus Brassica in the family Brassicaceae, and is a widely cultivated leafy vegetable in China, preferring a warm and humid environment.

[0003] Pectobacterium carotovorum subsp. carotovorum ( Peclobacterium carolovorum subsp. carolovorum ) is a common pathogen of Chinese cabbage soft rot. The pathogen can invade and cause disease through the seedlings or root seedlings of the plant. After the occurrence of soft rot, it will cause the decay and deterioration of Chinese cabbage plants, seriously affecting the yield of Chinese cabbage and restricting the development of Chinese cabbage planting industry. At present, chemical drugs are mostly used to control soft rot. The long-term application of traditional chemical agents has increasingly prominent hazards to the soil ecological environment, agricultural product safety and human and animal health. Biological control has the advantages of being green, safe, non-toxic and harmless, etc., which meets the requirements of sustainable agricultural development. It is an important direction and research hotspot for the control of vegetable soft rot and has broad application prospects. Summary of the Invention

[0004] In order to overcome the problems existing in the prior art, the present invention provides a microbial inoculant and its application.

[0005] To achieve the above object, the first aspect of the present invention provides a microbial inoculant, comprising the following components:

[0006] Component A: Penicillium restrictum ( Penicillium restrictum ) and Penicillium verruculosum ( Penicillium verruculosum );

[0007] Component B: sodium alginate, fulvic acid, extract of Panax japonicus Torr., extract of Polygonatum odoratum (Mill.) Druce, corn starch;

[0008] Among them, the addition amount of Penicillium restrictum is 1%-3% of the total weight of the microbial inoculant, and the addition amount of Penicillium verruculosum is 1%-5% of the total weight of the microbial inoculant, and the balance is Component B.

[0009] Further, the effective viable count of Penicillium restrictum and Penicillium verruculosum in Component A is 1x10 9 -2x10 9 CFU / g.

[0010] Further, the mass ratio of sodium alginate, fulvic acid, extract of Panax japonicus Torr., extract of Polygonatum odoratum (Mill.) Druce, corn starch in Component B is 6-8:90-120:2-3:1-4:6-9.

[0011] In the second aspect of the present invention, there is provided an application of the microbial inoculum of the first aspect in controlling Chinese cabbage soft rot, wherein the pathogen of the soft rot is *Pectobacterium carotovorum* Peclobacterium carolovorum subsp. carolo vorum ).

[0012] In the third aspect of the present invention, there is provided a method for controlling Chinese cabbage soft rot, which includes applying the microbial inoculum described in the first aspect to the rhizosphere of Chinese cabbage plant seedlings.

[0013] Through the above technical solutions, the present invention can at least achieve the following beneficial effects: The microbial inoculum provided by the present invention can effectively control Chinese cabbage soft rot caused by *Pectobacterium carotovorum* Peclobacterium carolovorum subsp. carolovorum ). Detailed Embodiments

[0014] Unless otherwise specified, the materials and reagents used in the present invention are commercially available.

[0015] Example 1

[0016] In the present invention, *Penicillium restrictum* and *Penicillium verruculosum* were purchased from Ningbo Mingzhou Biotechnology Co., Ltd. The product number of *Penicillium restrictum* is BMZ072236, and the product number of *Penicillium verruculosum* is BMZ074093; after activation and enlarged culture, the number of effective viable bacteria in *Penicillium restrictum* is about 1.5×10 10 CFU / g, and the number of effective viable bacteria in *Penicillium verruculosum* is about 2×10 10 CFU / g;

[0017] Sodium alginate in the present invention was purchased from Shandong Huiheng Biotechnology Co., Ltd.;

[0018] In the present invention, fulvic acid with CAS number 479-66-3 was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.;

[0019] In the present invention, extracts of *Panax japonicus* Torr. and *Polygonatum odoratum* (Mill.) Druce were purchased from Shanyang Lianfeng Biotechnology Co., Ltd., and the extracts are all water extracts with a specification of 10:1;

[0020] Corn starch is a common commercially available product.

[0021] Prepare the microbial inoculum according to Table 1 below:

[0022] Table 1 Microbial inoculum formula

[0023]

[0024] There is no particular limitation on the preparation method of the microbial inoculant. Any method of adding biocontrol bacteria to a composition in the art can be applied to the present invention. For example, Penicillium restrictum and Penicillium verruculosum can be first made into inoculants and then directly added to the aforementioned ingredients according to the proportions in the above table, or the Penicillium restrictum and Penicillium verruculosum can be packaged separately from the aforementioned ingredients and mixed or applied together before use. Another example is that Penicillium restrictum and Penicillium verruculosum can be cultured and expanded, and the obtained culture solution can be used to prepare the aforementioned inoculant according to a certain proportion. Considering the convenience during the preparation process and use, it is preferred to add Penicillium restrictum and Penicillium verruculosum to the aforementioned formula.

[0025] Example Two

[0026] Select three true-leaf Chinese cabbage seedlings with good growth and basically the same size, and use a suspension of Pectobacterium carotovorum subsp. carotovorum with a concentration of 1×10 8 CFU / mL to perform root irrigation on Chinese cabbage, with 200 mL of root irrigation for each plant. 7 days after root irrigation, apply the microbial inoculant prepared according to the formula in Table 1 to the Chinese cabbage plants after root irrigation, 30 g per plant; set 50 replicates for each formula, and investigate the disease index 14 days after applying the microbial inoculant. The results are shown in Table 2 below.

[0027] According to the disease grading standard in the "Guidelines for Field Efficacy Trials of Pesticides", the disease condition is graded according to the proportion of the lesion area on the leaf: grade 0 is 0; grade 1 is 1% - 5%; grade 3 is 6% - 10%; grade 5 is 11% - 25%; grade 7 is 26% - 50%; grade 9 is above 50%.

[0028] Disease index = Σ (number of diseased leaves at each level × disease level) / (total number of leaves investigated × highest disease level) × 100;

[0029] Table 2 Disease Index Table

[0030]

[0031] As can be seen from Table 2 above, the microbial inoculant of the present invention can effectively control the soft rot of Chinese cabbage caused by Pectobacterium carotovorum subsp. carotovorum and reduce the disease index of the soft rot of Chinese cabbage caused by Pectobacterium carotovorum subsp. carotovorum.

[0032] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A microbial agent, characterized in that: Contains the following components: Component A: Penicillium restrictum ( Penicillium restrictum ) and Penicillium verrucosum ( Penicillium verruculosum ); Component B: sodium alginate, fulvic acid, ginseng extract, polygonatum odoratum extract, corn starch; The amount of Penicillium restrictedum added is 1%-3% of the total weight of the microbial agent, the amount of Penicillium verrucosum added is 1%-5% of the total weight of the microbial agent, and the remainder is component B; the effective viable counts of Penicillium restrictedum and Penicillium verrucosum in component A are 1x10 9 -2x10 9 CFU / g; the mass ratio of sodium alginate, fulvic acid, Panax notoginseng extract, Polygonatum odoratum extract and corn starch in component B is 6-8:90-120:2-3:1-4:6-9; Panax notoginseng extract and Polygonatum odoratum extract are water extracts with a specification of 10:

1.

2. Use of the microbial agent according to claim 1 in preventing and treating soft rot of Chinese cabbage, wherein: The pathogen of the soft rot is Pectinobacterium carotovora Peclobacterium carolovorumsubsp.carolovorum ).

3. A method for preventing and treating soft rot of Chinese cabbage, characterized in that: The microbial agent according to claim 1 is applied to the rhizosphere of cabbage seedlings.

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