Probiotic composition for promoting alcohol degradation and metabolism and improving discomfort symptoms after drinking

Abstract

The invention relates to a probiotic composition for promoting alcohol degradation and metabolism and improving discomfort symptoms after drinking. The probiotic composition comprises the following raw materials in parts by weight: 35-40 parts of lactitol; 28 to 35 parts of fructo-oligosaccharide; 15 to 34 parts of stachyose; 5-10 parts of mango fruit powder; 2 to 4 parts of lactobacillus rhamnosus LR519; 0.5 to 2.5 parts of plant lactobacillus LP45 (Lipoprotein 45); 0.005 to 0.015 part of bifidobacterium bifidum B11, and 0.005 to 0.015 part of bacillus subtilis; 0.005 to 0.015 part of Lactobacillus helveticus L551, and 0.005 to 0.015 part of Lactobacillus And 0.1 to 0.3 part of vitamin C. The traditional Chinese medicine composition can promote alcohol degradation, accelerate alcohol metabolism and effectively improve discomfort symptoms after drinking.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial application, and in particular relates to a probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms. Background Art

[0002] Improper drinking often has the following hazards: 1) Headaches and dizziness after drinking; 2) Long-term drinking can also damage the nervous system, induce peripheral neuropathy and even cerebral cortical atrophy, and cause inattention and memory loss; 3) After drinking a lot of alcohol, vomiting will occur, and long-term vomiting will cause damage to the entire upper digestive tract; alcohol can penetrate the cell layer to destroy the gastric mucosa, making the gastric mucosa extremely susceptible to damage by gastric acid, various digestive enzymes, bile, etc., and then cause edema, erosion, and even bleeding and necrosis of the mucosal tissue; alcohol causes intestinal flora disorder and causes diarrhea, which in turn causes electrolyte disorder, angina and myocardial infarction, ischemic stroke, severe arrhythmia, sudden death, dehydration, acidosis and other serious complications. 4) Alcohol's metabolite acetaldehyde is a Class I carcinogen. Long-term or large-scale drinking will cause acetaldehyde to accumulate in the intestines and liver, leading to cancer of the liver and colon. The survey data on the symptoms of discomfort after drinking showed that: 1) Dizziness, headache, vomiting and nausea accounted for a large proportion, accounting for 84%, 60% and 62% respectively, while stomach pain, diarrhea, constipation and other symptoms accounted for a small proportion, accounting for 17%, 23% and 6% respectively (see Figure 1 ). 2) The proportion of dizziness, headache, nausea and vomiting in the 20-29 age group is relatively high, while gastrointestinal discomfort is relatively low or even absent; gastrointestinal discomfort symptoms begin to appear in the 30-39 age group when dizziness, headache, nausea and vomiting exist; the proportion of gastrointestinal discomfort symptoms in the population over 40 years old is higher than that of the first two groups (see Figure 2 ), indicating that gastrointestinal damage is positively correlated with drinking and aging. 3) Among the 30-49 age group, the number of people who experience four or more discomfort symptoms at the same time after drinking is the largest, while the proportion of people aged 20-29 who experience four or more discomfort symptoms at the same time is relatively small (see Figure 3 ). From the above survey results, it can be seen that the discomfort symptoms after drinking are a common phenomenon among all kinds of people. Therefore, how to promote the degradation of alcohol and improve the above discomfort symptoms caused by improper drinking is very important for people who often need to drink. Summary of the invention

[0003] The purpose of the present invention is to overcome the defects in the prior art and provide a probiotic composition that promotes alcohol degradation and metabolism and improves the discomfort symptoms after drinking. The probiotic composition can promote alcohol degradation, accelerate alcohol metabolism, and effectively improve the discomfort symptoms after drinking.

[0004] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0005] A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms, comprising the following raw materials in parts by weight:

[0006] Lactitol: 35-40 parts;

[0007] Fructo-oligosaccharides: 28-35 parts;

[0008] Stachyose: 15-34 parts;

[0009] Mango fruit powder: 5-10 portions;

[0010] Lactobacillus rhamnosus LR519: 2-4 parts;

[0011] Lactobacillus plantarum LP45: 0.5-2.5 parts;

[0012] Bifidobacterium bifidum B11: 0.005-0.015 parts;

[0013] Lactobacillus helveticus L551: 0.005-0.015 parts;

[0014] Vitamin C: 0.1-0.3 parts.

[0015] As a further technical solution, the Lactobacillus rhamnosus LR519 is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration Committee with a deposit number of CGMCC NO.15969; the Lactobacillus plantarum LP45 is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration Committee with a deposit number of CGMCC NO.8072; the Bifidobacterium bifidum B11 is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration Committee with a deposit number of CGMCC NO.24381; the Lactobacillus helveticus L551 is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration Committee with a deposit number of CGMCC NO.15604;

[0016] A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms, comprising the following raw materials in parts by weight: 38.06 parts of lactitol;

[0017] Fructooligosaccharides: 31.27 parts;

[0018] Stachyose: 19 parts;

[0019] Mango fruit powder: 7 parts;

[0020] Lactobacillus rhamnosus LR519: 2.78 parts;

[0021] Lactobacillus plantarum LP45: 1.67 parts;

[0022] Bifidobacterium bifidum B11: 0.01 part;

[0023] Lactobacillus helveticus L551: 0.01 parts;

[0024] Vitamin C: 0.2 parts.

[0025] The probiotic composition is used in preparing a product for sobering up and / or improving the discomfort symptoms after drinking.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The components of the probiotic composition of the present invention interact with each other, which can not only promote the rapid colonization of probiotics, increase the content of acetaldehyde dehydrogenase and alcohol dehydrogenase in the blood, and promote the degradation of ethanol and acetaldehyde in the blood, but also reduce the abundance of harmful bacteria in the gastrointestinal tract, increase the abundance of beneficial bacteria in the gastrointestinal tract, and promote the formation of biofilm, thereby improving the damaged intestinal barrier, reducing gastric inflammation, and improving most of the discomfort symptoms after drinking. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is the proportion of various discomfort symptoms after drinking in the total population;

[0029] Figure 2 This is a distribution diagram of discomfort symptoms after drinking among people of different age groups;

[0030] Figure 3 This is the distribution map of people who experience four or more discomfort symptoms at the same time after drinking;

[0031] Figure 4 A statistical diagram of the number of people whose sobering-up time is shortened after taking the probiotic composition of the present invention;

[0032] Figure 5 The effect of the probiotic composition of the present invention on the contents of ethanol, acetaldehyde, acetaldehyde dehydrogenase and alcohol dehydrogenase in blood;

[0033] exist Figure 5 In, a: ethanol; b: acetaldehyde; c: acetaldehyde dehydrogenase; d: alcohol dehydrogenase;

[0034] Figure 6 The effect of different dosages of the probiotic composition of the present invention on the ethanol content in the blood when the amount of alcohol consumed is the same;

[0035] Figure 7 The effect of different dosages of the probiotic composition of the present invention on the acetaldehyde content in the blood when drinking the same amount of alcohol;

[0036] Figure 8 The effect of different dosages of the probiotic composition of the present invention on the ADH content in the blood when the same amount of alcohol is consumed;

[0037] Fig. 9 The effect of different dosages of the probiotic composition of the present invention on the ALDH content in the blood when the amount of alcohol consumed is the same;

[0038] exist Figure 6-9 a: people who take four bags and drink half a catty to one catty of alcohol; b: people who take eight bags and drink half a catty to one catty of alcohol; c: people who take four bags and drink more than one catty of alcohol; d: people who take eight bags and drink more than one catty of alcohol;

[0039] Fig.10 The effect of the probiotic composition of the present invention on the α-diversity of intestinal flora in drinkers;

[0040] exist Fig.10 In the figure, a: Ace and Chao index of people weighing less than half a catty, b: Ace and Chao index of people weighing between half a catty and 1 catty; c: Ace and Chao index of people weighing more than 1 catty;

[0041] Fig.11 The effect of the probiotic composition of the present invention on the ASVsVenn diagram of drinkers;

[0042] exist Fig.11 In the middle, a: people weighing less than half a catty, b: people weighing between half a catty and 1 catty; c: people weighing more than 1 catty;

[0043] Fig.12 The effect of the probiotic composition of the present invention on the intestinal flora at the genus level;

[0044] exist Fig.12 a: people who drink less than half a catty; b: people who drink between half a catty and 1 catty; c: people who drink more than 1 catty; ZC1-5 / WZC1-5: people who drink less than half a catty by drinking / not drinking the probiotic composition of the present invention; ZC6-10 / WZC6-10: people who drink between half a catty and 1 catty by drinking / not drinking the probiotic composition of the present invention; ZC10 / WZC10: people who drink more than 1 catty by drinking / not drinking the probiotic composition of the present invention;

[0045] Fig.13 The effects of different dosages of the probiotic composition of the present invention on the phylum and genus levels of intestinal flora in people drinking half a catty to one catty of alcohol at the same drinking volume;

[0046] exist Fig.13 a: phylum level on the second day after drinking, b: genus level on the second day after drinking; ZC4 group: took 4 bags of the probiotic composition of the present invention before drinking, ZC8 group: took 8 bags of the probiotic composition of the present invention before drinking. DETAILED DESCRIPTION

[0047] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] In addition, it should be noted that the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0049] In the present invention,

[0050] Lactobacillus rhamnosus LR519 is deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration, with a deposit number of CGMCC NO.15969 and a deposit date of June 20, 2018; it has been disclosed in the patent with publication number CN110016442A.

[0051] Lactobacillus plantarum LP45 is deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration with a deposit number of CGMCC NO.8072 and a deposit date of August 26, 2013; it has been disclosed in the patent with publication number CN103642716A.

[0052] Bifidobacterium bifidum B11 is deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration, with a deposit number of CGMCC NO.24381 and a deposit date of January 20, 2022; it has been disclosed in the patent with publication number CN114886929A.

[0053] Lactobacillus helveticus L551 is deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration with a deposit number of CGMCC NO.15604 and a deposit date of April 11, 2018; it has been disclosed in the patent with publication number CN110241046A.

[0054] Unless otherwise specified, the raw materials used in the present invention are all commercially available.

[0055] Embodiment 1:

[0056] A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms, comprising the following raw materials in parts by weight:

[0057] Lactitol: 38.06 parts;

[0058] Fructooligosaccharides: 31.27 parts;

[0059] Stachyose: 19 parts;

[0060] Mango fruit powder: 7 parts;

[0061] Lactobacillus rhamnosus LR519: 2.78 parts;

[0062] Lactobacillus plantarum LP45: 1.67 parts;

[0063] Bifidobacterium bifidum B11: 0.01 part;

[0064] Lactobacillus helveticus L551: 0.01 parts;

[0065] Vitamin C: 0.2 parts.

[0066] Example 2

[0067] A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms, comprising the following raw materials in parts by weight:

[0068] Lactitol: 37 parts;

[0069] Fructo-oligosaccharide: 30 parts;

[0070] Stachyose: 17 parts;

[0071] Mango fruit powder: 7 parts;

[0072] Lactobacillus rhamnosus LR519: 2 parts;

[0073] Lactobacillus plantarum LP45: 1 part;

[0074] Bifidobacterium bifidum B11: 0.007 parts;

[0075] Lactobacillus helveticus L551: 0.007 parts;

[0076] Vitamin C: 0.1 parts.

[0077] Example 3

[0078] A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms, comprising the following raw materials in parts by weight: 39 parts of lactitol;

[0079] Fructo-oligosaccharide: 32 parts;

[0080] Stachyose: 20 parts;

[0081] Mango fruit powder: 9 parts;

[0082] Lactobacillus rhamnosus LR519: 3 parts;

[0083] Lactobacillus plantarum LP45: 2 parts;

[0084] Bifidobacterium bifidum B11: 0.012 parts;

[0085] Lactobacillus helveticus L551: 0.012 parts;

[0086] Vitamin C: 0.3 parts.

[0087] Application Example 1: Effect of the probiotic composition of the present invention on the relief of discomfort symptoms after drinking

[0088] 1. Test methods

[0089] A total of 100 people were recruited for this trial; two trials were conducted, and each person drank the same amount of alcohol in the two trials:

[0090] 1. Drinking alcohol but not taking the probiotic composition of the present invention: After drinking alcohol, investigate the discomfort symptoms that each person experiences, and count the number of people who experience each discomfort symptom;

[0091] 2. Drinking but taking the probiotic composition of the present invention: distribute the probiotic composition of the present invention prepared in Example 1, and take the product before and after each drinking. The amount of alcohol consumed by each person is the same when taking the probiotic composition of the present invention and when not taking the probiotic composition of the present invention;

[0092] II. Statistical indicators and results

[0093] 1. The present invention alleviates the discomfort symptoms after drinking on the day of drinking

[0094] The symptoms of discomfort after drinking were collected by questionnaire on the day of drinking. The test results are shown in Table 1.

[0095] Table 1: Statistical results of symptoms of alcohol discomfort on the day of drinking

[0096]

[0097] After drinking a lot of alcohol, the body will experience a variety of discomfort symptoms: dizziness, headache, nausea and vomiting, acid reflux, stomach pain, diarrhea, constipation, blushing, etc. From the above data, it can be seen that after taking the probiotic composition product of the present invention, the total effective rate of the above symptoms completely disappearing can reach 51%. For the improvement of discomfort symptoms after drinking, the total effective rate is 84%.

[0098] 2. The present invention alleviates the discomfort symptoms after drinking on the second day after drinking

[0099] A questionnaire was used to collect statistics on the symptoms of alcohol discomfort on the second day after drinking. The test results are shown in Table 2.

[0100] Table 2: Symptoms of alcohol-induced discomfort on the second day after drinking

[0101]

[0102] After drinking a lot of alcohol, the body will experience a variety of discomfort symptoms such as drowsiness, dizziness, headache, nausea and vomiting, acid reflux, stomach pain, diarrhea, constipation, etc. Some of these symptoms can last until the second day after drinking. From the above data, it can be seen that after taking the probiotic composition product of the present invention, the improvement of the drowsiness symptom is not very obvious, with an effective rate of 42%; headache, dizziness, nausea and vomiting, acid reflux, stomach pain, diarrhea, constipation and other symptoms are significantly improved, with a total effective rate of 69%;

[0103] 3. Improvement of the sobering time by the probiotic composition of the present invention

[0104] A questionnaire was used to investigate the sobering time after drinking (the definition of sobering up: the time from drinking the same amount of alcohol at the same time to waking up naturally the next day), and the number of people whose sobering time was shortened after taking the probiotic composition of the present invention was counted. The results are shown in Figure 4 ;

[0105] Statistical results show that after taking the probiotic composition of the present invention, 77% of people's sobering time is shortened (see Figure 4 ), shorten the time to 1h-12h.

[0106] Application Example 2: Effect of the probiotic composition of the present invention on body metabolism

[0107] 1. Test methods

[0108] Test reagents: probiotic composition product of the present invention, alcohol content test kit, acetaldehyde content detection, alcohol dehydrogenase test kit, acetaldehyde dehydrogenase test kit, liquor;

[0109] Test population (35 people): selected people who are over 18 years old, have no major diseases, are not alcohol-dependent, do not take other nutritional supplements regularly, and do not take antibiotics within three days of the test.

[0110] Test 1: Drinking the probiotic composition product of the present invention, without specifying the amount of alcohol consumed, drinking normally, and recording the amount of alcohol consumed;

[0111] Experiment 2: The same population was repeated, drinking the same amount of alcohol as in Experiment 1.

[0112] The alcohol consumption of the experimental population is shown in Table 3;

[0113] Table 3 Alcohol consumption of experimental population

[0114] Alcohol consumption Number of people 0-half catty (including half catty) 10 Half a catty-1 catty (including 1 catty) 17 More than 1 catties 8

[0115] The situation of the test population taking the probiotic composition of the present invention is shown in Table 4;

[0116] Table 4: The situation of the test group taking the probiotic composition of the present invention

[0117]

[0118]

[0119] Both Experiment 1 and Experiment 2 were conducted at three time periods: 0h before, 0.5h, and 12h after drinking. 2-3ml of venous blood was collected for testing of alcohol dehydrogenase, acetaldehyde dehydrogenase, serum alcohol content, and acetaldehyde content.

[0120] Note: The types and quantities of food eaten during the two experiments were consistent, and the amount of water drunk was kept consistent as much as possible.

[0121] 2. Test indicators and results

[0122] 1. The improvement of the probiotic composition of the present invention on body metabolism, the results are shown in Figure 5 ;

[0123] Figure 5 is the change level (average value) of ethanol, acetaldehyde, acetaldehyde dehydrogenase and alcohol dehydrogenase in the blood of the test population. Figure 5 It can be seen that the detection index results of the three selected time points, acetaldehyde content and ethanol content levels, reached a peak in the blood 30 minutes after drinking, and after drinking the probiotic composition of the present invention, the levels of the two indicators decreased, the ethanol content level decreased by 48.37%, and the acetaldehyde content level decreased by 29.41%. The levels of alcohol dehydrogenase and acetaldehyde dehydrogenase increased with the increase of ethanol content and acetaldehyde content levels, respectively. After drinking the probiotic composition of the present invention, alcohol dehydrogenase and acetaldehyde dehydrogenase increased significantly, with an increase ratio of 22.69% for alcohol dehydrogenase and 41.20% for acetaldehyde dehydrogenase. Comparative analysis of the average values ​​of the four indicators showed that the ethanol content decreased by nearly 50%, but the increase rate of alcohol dehydrogenase was about 23%. It can be speculated that the intake of the probiotic composition of the present invention promoted the production of alcohol dehydrogenase in the liver of the body on the one hand, and promoted the rapid colonization of the strain on the other hand, which helped the decomposition of ethanol, because the strain helped the body to decompose alcohol in the intestine, which was intracellular, thus confirming this phenomenon; compared with promoting the production of alcohol dehydrogenase, the intake of the probiotic composition of the present invention will further promote the production of acetaldehyde dehydrogenase in the body, thereby helping the body to decompose acetaldehyde.

[0124] 2. Improvement of metabolism in different dosages under the same alcohol consumption

[0125] 1) Improvement of ethanol metabolism, see Figure 6 ;

[0126] When drinking the same amount of alcohol, when drinking half a catty to one catty of liquor, drinking four bags of the probiotic composition of the present invention and eight bags of the probiotic composition of the present invention respectively, the ethanol change level is as follows Figure 6 As shown, the peak ethanol content decrease rate of the combination of the present invention after drinking four bags was 54%, while the peak ethanol content of the combination of the present invention after drinking eight bags was 61%; the area under the curve calculation result showed that the improvement rate of ethanol metabolism within the second day (12h) of drinking four bags was 54%, while the improvement rate of eight bags was 60%. With the same drinking amount, when drinking more than 1 jin of liquor, the peak ethanol content change decrease rate of four bags and eight bags of the probiotic composition of the present invention was 37% and 31% respectively; the improvement rate of the area under the curve was 57% and 22% for four bags and eight bags respectively.

[0127] Therefore, when drinking the same amount of liquor, when drinking half a catty to 1 catty, the improvement level of taking eight bags will be greater than that of taking four bags, and when the amount of alcohol consumed is greater than 1 catty, taking four bags will have a better effect. The results once again prove that at high doses of alcohol concentration, the effect of the probiotic composition of the present invention will decrease.

[0128] 2) Improvement of acetaldehyde metabolism, see Figure 7 ;

[0129] The same amount of alcohol consumption, when drinking half a catty to one catty of liquor, drinking four bags of the probiotic composition of the present invention and eight bags of the probiotic composition of the present invention, the change level of acetaldehyde is as follows Figure 7 As shown, the acetaldehyde peak value decrease rate after drinking four bags of the probiotic composition of the present invention is 34%, while the acetaldehyde peak value after drinking eight bags of the probiotic composition of the present invention is 19%; the area under the curve calculation result shows that the acetaldehyde metabolism improvement ratio within the second day (12h) of drinking is 36% when taking four bags, while the improvement rate of taking eight bags is 17%. With the same drinking amount, when drinking more than 1 jin of liquor, the acetaldehyde peak value content change decrease rate is 52% and 26% when drinking four bags and eight bags of the probiotic composition of the present invention respectively; the area under the curve improvement rate is 44% and 27% when taking four bags and taking eight bags respectively.

[0130] Therefore, when drinking the same amount of liquor, when drinking half a catty to one catty or drinking more than one catty, the effect of taking four bags is better in terms of the improvement level of acetaldehyde.

[0131] 3) Improvement of alcohol dehydrogenase (ADH) metabolism, see Figure 8 ;

[0132] When drinking the same amount of alcohol, when drinking half a catty to one catty of liquor, drinking four bags of the probiotic composition of the present invention and eight bags of the probiotic composition of the present invention respectively, the ADH change level is as follows Figure 8 As shown, the peak growth rate of ADH content is 25% when drinking four bags, while the peak growth rate of ADH content is 16% when drinking eight bags; the area under the curve calculation result shows that the improvement rate of ADH metabolism within the second day (12h) of drinking is 27% when taking four bags, while the improvement rate is 15% when taking eight bags. With the same drinking amount, when drinking more than 1 jin of liquor, the growth rates of ADH peak content are 22% and 14% when drinking four bags and eight bags of the probiotic composition of the present invention respectively; the improvement rates of the area under the curve are 24% and 17% when taking four bags and eight bags respectively.

[0133] Therefore, when drinking the same amount of liquor, when drinking half a catty to one catty or drinking more than one catty, the effect of taking four bags is better in terms of ADH improvement level.

[0134] 4) Improvement of acetaldehyde dehydrogenase (ALDH) metabolism, see Fig. 9 ;

[0135] The same amount of alcohol consumption, when both drank half a catty to one catty of liquor, and drank four bags of the probiotic composition of the present invention and eight bags of the probiotic composition of the present invention, the ALDH level changes as above Fig. 9 As shown, the peak growth rate of ALDH content was 39% when four bags were consumed, while the peak growth rate of ALDH content was 35% when eight bags were consumed; the area under the curve was calculated, and the improvement rate of ALDH metabolism within the second day (12h) of drinking was 41% when four bags were consumed, while the improvement rate reached 34% when eight bags were consumed. With the same amount of alcohol consumed, when drinking more than 1 jin of liquor, the growth rates of ALDH peak content were 51% and 35% when four bags and eight bags of the probiotic composition of the present invention were consumed respectively; the improvement rates of the area under the curve were 50% and 39% when four bags were consumed and eight bags were consumed respectively.

[0136] Therefore, when drinking the same amount of liquor, when drinking half a catty to one catty or drinking more than one catty, in terms of the improvement level of ALDH, taking four bags is more effective.

[0137] Application Example 3: Effect of the probiotic composition of the present invention on intestinal flora

[0138] 1. Test methods

[0139] Test sample: feces; the final decision was made based on the results of the previous experiment, and 24 people were randomly selected for follow-up experiments. The collected samples were sent to Major Bio for testing, and the pre-processed data was uploaded to the Major Bio cloud platform (https: / / cloud.majorbio.com) for data analysis.

[0140] The drinking amount of the selected test population is shown in Table 5, and the taking of the probiotic composition of the present invention is shown in Table 6;

[0141] Table 5

[0142] Alcohol consumption Number of people 0-half catty (including half catty) 6 Half a catty-1 catty (including 1 catty) 12 More than 1 catties 6

[0143] Table 6

[0144]

[0145] 2. Test indicators and results

[0146] 1. Alpha diversity of intestinal flora of people who drank the probiotic composition of the present invention with different alcohol consumption, the results are shown in Figure 10-11 ;

[0147] 1) Chao and Ace indices reflect community richness. Fig.10 a and Fig.10 As shown in b, within 1 jin, taking the probiotic composition of the present invention before drinking can increase the community diversity level, but above 1 jin, taking the probiotic composition of the present invention cannot increase the community diversity. The possible reason is that the intake of a large amount of prebiotics provides a C source for harmful bacteria.

[0148] 2) Each deduplicated sequence generated after denoising using DADA2 is called an amplicon sequence variant (ASVs), which is equivalent to clustering at 100% similarity. The greater the number of ASVs, the greater the diversity of the microbial community. Draw a Venn diagram of ASVs, such as Fig.11 As shown in the figure, the number of unique ASVs in the ZC and WZC groups below 50 kg were 1129 and 908, respectively, and the number of unique ASVs in the ZC and WZC groups between 50 kg and 1 catties were 1487 and 1477, respectively. Alcohol can reduce the diversity of intestinal flora in people weighing less than 1 catties. The difference in the number of ASVs in each group indicates that there are differences in the similarity of intestinal flora in each group.

[0149] 2. Analysis of the abundance of intestinal flora in people who drank the probiotic composition of the present invention with different alcohol consumption. The results are shown in Fig.12 ;

[0150] The drinking population is divided into three categories: ZC1-5 / WZC1-5: people who drink less than half a catty of alcohol with / without drinking the probiotic composition of the present invention; ZC6-10 / WZC6-10: people who drink between half a catty and one catty of alcohol with / without drinking the probiotic composition of the present invention; ZC10 / WZC10: people who drink more than one catty of alcohol with / without drinking the probiotic composition of the present invention.

[0151] like Fig.12 As shown in a, when the amount of alcohol consumed is less than half a catty, taking the probiotic composition of the present invention before drinking can significantly increase the abundance of Roseburia by 128%, Bifidobacterium by 209%, Paraprevotella by 55% and Prevotella by 28%. Roseburia can improve intestinal biodiversity, improve glucose tolerance, help lose weight, and revitalize colon cells; Bifidobacterium helps improve digestive problems, is antibacterial and anti-viral, anti-inflammatory, improves blood sugar control, lowers blood lipid levels, and improves immunity; Prevotella can help decompose proteins and carbohydrates in the human body, and is related to the synthesis of short-chain fatty acids (SCFAs), which can promote intestinal peristalsis and reduce the inflammatory response of intestinal cells; Paraprevotella has certain similarities with certain bacteria of the genus Prevotella, which helps maintain intestinal homeostasis and prevent pathogen infection.

[0152] like Fig.12As shown in a, when the amount of alcohol consumed is less than half a catty, the reduction rate of Haemophilus is 62%, the reduction rate of Bilophila is 73%, the reduction rate of Lachnoclostridium is 53%, and the reduction rate of Lachnospiraceae is 27%. In the data, it is found that one of the people has a very significant increase of 291% in abundance after taking the probiotic composition of the present invention. Haemophilus is a Gram-negative rod or coccobacillus of the Proteobacteria. The pathogen can cause primary suppurative infection and severe secondary infection. Its overgrowth is related to the occurrence of some inflammatory bowel diseases, meningitis and metabolic diseases. Bilophila is an anaerobic, Gram-negative, bile-resistant, catalase-positive rod. It is an opportunistic pathogen, and its increased abundance has been shown to have a negative impact on intestinal inflammation. It synergizes with a high-fat diet to promote higher inflammatory responses, intestinal barrier dysfunction, and abnormal bile acid metabolism, leading to higher abnormal glucose metabolism and hepatic fat. The relative abundance of Lachnoclostridium increases in enteritis lesions and intestinal tumors, and in liver steatosis and metabolic diseases. Lachnospirium is a Gram-negative spiral bacterium, and its infection can cause chronic gastritis, leading to inflammation and tissue damage of the gastric mucosa, and also induces host immune responses.

[0153] like Fig.12 As shown in Figure b, when the amount of alcohol consumed is half a catty to one catty, taking the probiotic composition of the present invention before drinking can significantly increase the abundance of Anaerostipes by 63%, Acidaminococcus by 407%, Coprococcus by 10%, Prevotella_9 by 27%, Paraprevotella by 303% and Bifidobacterium by 401%. Figure 6As shown in Figure b, when the amount of alcohol consumed was half a catty to one catty, the changes in harmful bacteria were as follows: after taking the probiotic composition of the present invention, the reduction rate of Haemophilus was 16%, the reduction rate of Bilophila was 9%, the reduction rate of Lachnoclostridium was 13%, and the reduction rate of Lachnospiraceae was 46%. Only one person detected the presence of Lactobacillus reuteri. After taking the probiotic composition of the present invention, the abundance of Lactobacillus reuteri increased significantly, with a growth rate of 728%. Coprococcus can produce bacteriocins and other antibacterial substances to inhibit the growth of pathogens such as Escherichia coli and Salmonella, improve the intestinal microenvironment, repair the intestinal barrier, reduce the content of intestinal urease and endotoxin, and reduce the content of ammonia and endotoxin in the blood, so as to reduce the damage of inflammatory factors to the liver; Acidococcus can compete for nutrition and living space, promote the activity of immune cells, enhance the function of the immune system, thereby inhibiting the growth of harmful bacteria (such as pathogenic bacteria or spoilage bacteria) and maintaining the balance of intestinal flora. There are many studies on the genus Anaerobic Corynebacterium fecalis in the treatment and prevention of diseases. A double-blind experiment published in Gut Microbes in 2023 proved that its strains were positively correlated with constipation, so this also provides theoretical support for the probiotic composition of the present invention to relieve constipation; Bifidobacterium helps to improve digestive problems, antibacterial and viral, anti-inflammatory, improve blood sugar control, lower blood lipid levels, improve immunity, show antioxidant activity, help prevent eczema, relieve stress and allergies; Lactobacillus reuteri is a Gram-positive rod-shaped bacterium with strong adhesion to the intestinal mucosa, good intestinal barrier repair ability, antagonism of harmful bacteria colonization, regulation of intestinal flora and other probiotic functions, to avoid intestinal diseases. Moreover, it can produce a non-protein broad-spectrum antibacterial substance called "Reuterin", which can widely inhibit the growth of bacteria, fungi and pathogens.

[0154] like Fig.12As shown in Figure c, when the amount of alcohol consumed is more than 1 jin, after taking the probiotic composition of the present invention before drinking, the abundance of Bifidobacterium, Prevotella_9 and Anaerostipes can be significantly increased by 163%, 59% and 310% respectively. The changes in harmful bacteria are that the reduction rate of Bilophila, Lachnoclostridium and Lachnospiraceae is 43%, 55% and 43% respectively. However, one strain of harmful bacteria Megamonas has been significantly increased in the dominant flora. Megamonas is a core species in the intestine and is a characteristic of Asia. It is closely related to diseases such as inflammatory bowel disease, colorectal cancer and obesity. This may be because, after the amount of alcohol consumed reaches more than 1 jin, these people are long-term drinkers, so the intestinal flora has been destroyed, and one sample data has changed abnormally. After taking the probiotic composition of the present invention, the abundance has increased significantly by 219%. The probiotic composition of the present invention has almost no effect on improving the intestinal tract, and due to excessive intake of prebiotics, it may also promote the increase of some harmful bacteria.

[0155] 3. Changes in intestinal flora of people who drank the probiotic composition of the present invention with the same amount of alcohol, see Fig.13 ;

[0156] The ratio of Firmicutes to Bacteroidetes is also called the F / B ratio, which is often used as an indicator of obesity and metabolic disorders. The F / B ratio of obese people will increase significantly. Fig.13 a and Fig.13 As shown in Figure 2, taking the probiotic composition of the present invention before drinking alcohol can significantly reduce the ratio of Firmicutes to Bacteroidetes at the phylum level, which can indicate that as the specifications of the probiotic composition of the present invention increase, the F / B ratio can be reduced and metabolic barriers can be improved. The intake of eight bags of the probiotic composition of the present invention can significantly reduce the level of Fusobacteria, and Fusobacterium species may be associated with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.

[0157] At the genus level, the intake of eight bags of the probiotic composition of the present invention can reduce the level of Megamonas to 0. Megamonas is a core species in the intestine and is a characteristic of Asia. It is closely related to diseases such as inflammatory bowel disease, colorectal cancer, and obesity. The intake of eight bags of the probiotic composition of the present invention significantly increases Prevotella (Prevotella_9), which is also related to the synthesis of short-chain fatty acids (SCFAs). Short-chain fatty acids can promote intestinal peristalsis and reduce the inflammatory response of intestinal cells. At the genus level, the intake of eight bags of the probiotic composition of the present invention can reduce the level of Megamonas to 0, and significantly increase the level of Faecalibacterium, which is called the next generation of probiotics for preventing inflammation. It is one of the important producers of butyric acid, has anti-inflammatory effects, maintains the activity of bacterial enzymes, and protects the digestive system from intestinal pathogens.

[0158] In summary, the beneficial effects of the present invention are:

[0159] 1) Taking the probiotic composition of the present invention can effectively relieve multiple discomfort symptoms after drinking, and has a relieving effect on various discomfort symptoms the next day after drinking, can reduce the situation where multiple symptoms occur at the same time, and shorten the sobering time. In addition, since people of different age groups show different main discomfort symptoms after drinking, among which gastrointestinal discomfort symptoms are more prominent with age, therefore, the effectiveness of the product can be enhanced according to the different symptom relief needs of different groups of people.

[0160] 2) After taking the probiotic composition of the present invention, the alcohol concentration in the blood can be reduced by nearly half (48.37%) and the acetaldehyde concentration can be reduced by one third (29.41%) 30 minutes after drinking. Therefore, it can be seen that taking the probiotic composition of the present invention can help the body metabolize alcohol and acetaldehyde, and on the one hand, it can relieve the discomfort caused by alcohol and acetaldehyde to the body (headache, dizziness, stomach discomfort and other symptoms); on the other hand, after taking the probiotic composition of the present invention, the blood acetaldehyde dehydrogenase (22.69%) and the content of acetaldehyde dehydrogenase (41.2%) can be increased, thereby improving the alcohol tolerance; and the improvement of alcohol tolerance varies from person to person, and from the perspective of enzyme levels, it can be increased to 20%-50% of the usual level. The intake of the probiotic composition of the present invention promotes the production of alcohol dehydrogenase in the liver of the body on the one hand, and promotes the rapid colonization of the strain on the other hand, which helps the decomposition of ethanol, because the strain helps the body to decompose alcohol in the intestine, which is decomposed intracellularly, thus confirming this phenomenon; compared with promoting the production of alcohol dehydrogenase, the intake of the probiotic composition of the present invention will further promote the production of acetaldehyde dehydrogenase in the body, thereby helping the body to decompose acetaldehyde.

[0161] 3) Regarding the improvement of drinking different doses of the probiotic composition product of the present invention with the same amount of alcohol, when drinking half a catty to 1 catty, eight bags of the probiotic composition of the present invention will have a greater improvement level than four bags, and when drinking more than 1 catty, four bags will have a better effect. The results once again prove that at high-dose alcohol concentrations, the effect of the probiotic composition of the present invention will decrease; when drinking the same amount of liquor, when drinking half a catty to 1 catty and drinking more than 1 catty, four bags will have a better effect on the improvement level of acetaldehyde, acetaldehyde dehydrogenase and alcohol dehydrogenase.

[0162] 4) After taking the probiotic composition of the present invention, the Alpha-diversity levels of all populations at the genus and phylum levels were increased by 6%; from the species Venn diagram analysis, it was found that taking the probiotic composition of the present invention before drinking can increase unique species from the genus to the phylum level, and alcohol can reduce the diversity of intestinal flora. The difference in the number of each group indicates that there is a difference in the similarity of the intestinal flora of each group. However, at the ASV level, the intake of the probiotic composition of the present invention did not increase the species level, which may be related to the amount of alcohol consumed. Therefore, subsequent analysis was conducted on people with different amounts of alcohol consumed. Taking the probiotic composition of the present invention before drinking can reduce the level of Proteobacteria and increase the level of Actinobacteriota at the phylum level. At the subordinate level, it was found that the intake of the probiotic composition of the present invention can significantly increase Bifidobacterium, with an increase in abundance of 294%, and the abundance of lactic acid bacteria (Coprococcus, Acidaminococcus, Limosilactobacillus and Romboutsia) increased significantly by 243%. Changes in the abundance of some harmful bacterial genera were also found, among which Bilophila, Lachnoclostridium, and Lachnospira were significantly reduced by 49%, 39%, and 39%, respectively. Therefore, taking the probiotic composition of the present invention after drinking can reduce intestinal inflammatory response, abnormal bile acid metabolism, and the occurrence of liver fat; however, Phascolarctobacterium was reduced, which may be due to: the intake of oligofructose reduced its abundance level, and the level of this genus was closely related to the level of Bacteroides, so the intake of the probiotic composition of the present invention reduced the levels of Phascolarctobacterium and Bacteroides; the BugBase phenotype was predicted, and after taking the probiotic composition of the present invention before drinking, the phenotype of biofilm formation was significantly increased, thereby improving the damaged intestinal barrier.

[0163] 5) In order to find the key strains that cause intestinal disorders due to drinking, the intestinal flora of people who do not drink the probiotic composition of the present invention but only drink alcohol was analyzed, and the drinking population was divided into three categories: people who drink less than half a catty; people who drink between half a catty and one catty; people who drink more than one catty. Under different drinking amounts, the abundance of lactic acid bacteria (Coprococcus, Acidaminococcus, Limosilactobacillus, and Romboutsia) showed an increasing trend, indicating that the intake of the probiotic composition of the present invention can improve the growth of lactic acid bacteria in the intestine. Among them, when the drinking amount is less than half a catty, the abundance of lactic acid bacteria increases by 67%, increases by 452% when half a catty to one catty, and increases by 17% when more than one catty. The increase in the abundance of lactic acid bacteria in the intestine is beneficial to help the human body decompose ethanol and reduce the inflammatory response and liver damage caused by drinking. The horizontal abundance of Bifidobacterium is reduced to less than 5, which is almost negligible. Among harmful bacteria, Haemophilus can cause nausea, dizziness and headache. After taking the probiotic composition of the present invention (drinking less than half a catty and half a catty to 1 catty), its abundance is significantly reduced by 62% and 16%. Bilophilus can cause abnormal bile acid metabolism and liver fat. After taking the probiotic composition of the present invention at different drinking amounts, its abundance is significantly reduced by 73%, 9% and 43%. Studies have shown that Lachnoclostridium has a high relative abundance in liver fatty degeneration and metabolic diseases. At different drinking amounts, its abundance is significantly reduced by 53%, 13% and 55% after taking the probiotic composition of the present invention. Infection with Lachnospira can cause chronic gastritis. At different drinking amounts, its abundance is significantly reduced by 27%, 46% and 43% after taking the probiotic composition of the present invention, indicating that taking the probiotic composition of the present invention can reduce gastric inflammation and mediate its damage to the intestines and internal organs.

[0164] 6) To verify the effect of the intake of multiple specifications of the probiotic composition of the present invention, the present invention analyzes the intestinal flora of people with similar alcohol consumption but different intakes of the probiotic composition of the present invention. Taking the probiotic composition of the present invention before drinking can significantly reduce the ratio of Firmicutes to Bacteroidetes at the phylum level, and as the specifications of the probiotic composition of the present invention increase, the F / B ratio can be reduced, and metabolic obstruction can be improved; the intake of eight bags of the probiotic composition of the present invention can reduce the level of Megamonas to 0, and significantly increase the level of Faecalibacterium, which is called the next generation of probiotics for preventing inflammation. Therefore, if the amount of alcohol consumed is less than 1 catty, taking a higher specification of the probiotic composition of the present invention will have a better improvement effect.

[0165] The above-described embodiments are only preferred embodiments of the present invention, and are not exhaustive of the feasible implementations of the present invention. For those skilled in the art, any obvious changes made thereto without departing from the principles and spirit of the present invention should be considered to be included in the scope of protection of the claims of the present invention.

Claims

1. A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms, characterized in that: The following raw materials are included in parts by weight: Lactitol: 35-40 parts; Fructo-oligosaccharides: 28-35 parts; Stachyose: 15-34 parts; Mango fruit powder: 5-10 portions; Lactobacillus rhamnosus LR519: 2-4 parts; Lactobacillus plantarum LP45: 0.5-2.5 parts; Bifidobacterium bifidum B11: 0.005-0.015 parts; Lactobacillus helveticus L551: 0.005-0.015 parts; Vitamin C: 0.1-0.3 parts.

2. A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms according to claim 1, characterized in that: The Lactobacillus rhamnosus LR519 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with the deposit number of CGMCC NO.15969; The plant lactobacillus LP45 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with the deposit number of CGMCCNO.8072; The Bifidobacterium bifidum B11 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration with the deposit number of CGMCCNO.24381; The Lactobacillus helveticus L551 is deposited in the General Microbiological Center of China National Committee for the Preservation of Microbiological Cultures with the deposit number of CGMCCNO.15604.

3. A probiotic composition for promoting alcohol degradation and metabolism and improving alcohol discomfort symptoms according to claim 1, characterized in that: The following raw materials are included in parts by weight: Lactitol: 38.06 parts; Fructooligosaccharides: 31.27 parts; Stachyose: 19 parts; Mango fruit powder: 7 parts; Lactobacillus rhamnosus LR519: 2.78 parts; Lactobacillus plantarum LP45: 1.67 parts; Bifidobacterium bifidum B11: 0.01 part; Lactobacillus helveticus L551: 0.01 parts; Vitamin C: 0.2 parts.

4. Use of the probiotic composition according to any one of claims 1 to 3 in the preparation of a product for sobering up and / or improving symptoms of alcohol abuse.

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