Anti-hair loss and oil control composition for asian men and use thereof

Abstract

The application discloses a hair loss prevention and oil control composition suitable for Asian men and application thereof, and relates to the technical field of cosmetics.The composition comprises the following components in parts by weight: 0.5-10 parts of Melissa officinalis extract, 0.05-3 parts of Sarcandra glabra extract, 0.02-3 parts of Chamaecyparis obtusa extract and 0.05-0.8 parts of black willow bark extract.The Melissa officinalis extract, the Sarcandra glabra extract, the Chamaecyparis obtusa extract and the black willow bark extract are combined to have a synergistic effect, the composition formed by the specific combination of the components can improve the hair loss problem of sensitive skin from multiple dimensions of comprehensive mood regulation, cell autophagy activation, oil control and soothing;the hair loss prevention composition combines physiological hair loss prevention components and psychological mood soothing components, realizes double intervention on the hair loss problem, and is more suitable for Asian men with sensitive skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an anti-hair loss and oil-control composition suitable for Asian men and its application. Background Technology

[0002] With the ever-increasing pace of life and growing pressure from work and life, hair loss has become a common problem faced by many people, and it is showing a trend of affecting younger people. The scalp is part of the skin and has a similar structure to facial skin. However, the scalp is more delicate than facial skin. Compared to the cheeks, the scalp is thinner but has more sebaceous glands, meaning it is more sensitive and produces more oil. Therefore, the scalp requires more attention to care, and the care products used should be more mindful of potential sensitivity issues.

[0003] In the men's hair loss prevention product market, existing technologies largely rely on chemically synthesized ingredients. While these products can alleviate hair loss to some extent, they often neglect the specific needs of sensitive skin. Furthermore, hair loss is frequently associated with emotional stress, especially for men with sensitive skin. Existing hair loss prevention products typically focus on a single effect, such as oil control or promoting hair growth, lacking a comprehensive consideration of multiple benefits including mood regulation, cellular autophagy activation, and soothing. Therefore, developing a hair loss prevention product that can simultaneously address both physiological and psychological aspects is of great significance.

[0004] It can be inferred that incorporating mood-regulating ingredients into anti-hair loss products, in synergy with oil-controlling, autophagy-activating, anti-inflammatory, and soothing ingredients, can effectively improve hair loss in men caused by excessive stress. Furthermore, the selected active ingredients are all plant-based, making them more suitable for people with sensitive skin.

[0005] Existing anti-hair loss products have limited functionality, with most targeting only hair loss and neglecting other important factors such as mood regulation and skin soothing. Furthermore, to enhance their effectiveness, many existing anti-hair loss products add multiple active ingredients, overlooking the needs of Asian men with sensitive skin. Therefore, finding products suitable for Asian men with sensitive skin that address both hair loss and oil control is a pressing technical challenge in this field. Summary of the Invention

[0006] Based on this, the purpose of this invention is to overcome the shortcomings of the prior art and provide a hair loss prevention and oil control composition and application suitable for Asian men. This composition can improve hair loss problems of Asian men with sensitive skin from multiple dimensions, including comprehensive mood regulation, activation of cell autophagy, oil control, and soothing. This hair loss prevention composition integrates physiological hair loss prevention ingredients and psychological mood-soothing ingredients, achieving dual intervention for hair loss problems. In addition, all ingredients are selected from plant sources, making it more suitable for use by Asian men with sensitive skin.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect, the present invention provides a hair loss prevention and oil control composition suitable for Asian men, the composition comprising the following ingredients in parts by weight: 0.5-10 parts of lemon balm extract, 0.05-3 parts of coral bark extract, 0.02-3 parts of cypress leaf extract, and 0.05-0.8 parts of black willow bark extract.

[0009] Lemon balm extract can influence neuroregulatory factors such as dopamine and cortisol, achieving mood-based skincare. Furthermore, lemon balm extract possesses strong antioxidant and free radical scavenging activity, effectively reducing oxidative stress damage in nerve cells, thereby improving mood by reducing oxidative stress in nerve cells. The diterpenoid glycosides in coralberry extract have significant anti-inflammatory effects on the skin, reducing redness. The physalis extract contains physalisol, which can enhance autophagy activity in human dermal papilla cells and mitochondrial autophagy, promoting dermal papilla cell proliferation. The active ingredients in black willow bark extract can affect sebaceous gland secretion, achieving oil control. Its phenylethyl glycosides can inhibit fat production and also suppress the production of inflammatory factors, providing a soothing effect.

[0010] This invention has found that when the above-mentioned plant extracts are used in combination in specific amounts, they can improve hair loss problems in Asian men with sensitive skin from multiple dimensions, including comprehensive mood regulation, activation of autophagy, oil control, and soothing. This anti-hair loss composition combines anti-hair loss ingredients at the physiological level with mood-soothing ingredients at the psychological level, achieving dual intervention for hair loss problems. Furthermore, all ingredients are plant-based, making it more suitable for use by Asian men with sensitive skin.

[0011] In a preferred embodiment of the first aspect, the mass ratio of the sum of lemon balm extract and gorgonian coral extract to the sum of the mass of cypress leaf extract and black willow bark extract in the composition is: (lemon balm extract + gorgonian coral extract) : (cypress leaf extract + black willow bark extract) = (1.1-8) : (0.6-2.5). For example, the mass ratio of (lemon balm extract + gorgonian coral extract) : (cypress leaf extract + black willow bark extract) can be, but is not limited to, 1.1:0.6, 1.5:1, 2:2, 4:1, 6:2, 8:2, or 8:2.5.

[0012] As a preferred embodiment of the first aspect, the mass ratio of the sum of lemon balm extract and coral extract to the sum of the mass of cypress leaf extract and black willow bark extract in the composition is: (lemon balm extract + coral extract) : (cypress leaf extract + black willow bark extract) = (2.3-6.7) : (0.9-1.9).

[0013] As a preferred embodiment of the first aspect, the mass ratio of the sum of lemon balm extract and coral extract to the sum of the mass of cypress leaf extract and black willow bark extract in the composition is: (lemon balm extract + coral extract) : (cypress leaf extract + black willow bark extract) = 4.5 : 1.3.

[0014] This invention has found that the mass ratio of lemon balm extract and gorgonian coral extract to the mass ratio of cypress leaf extract and black willow bark extract in the anti-hair loss and oil-control composition not only affects the TNF-α inhibition rate of the composition, thus affecting its anti-inflammatory and oxidative stress protective effects, but also influences the composition's improvement on a* and TEWL values, thereby affecting its soothing and skin barrier repair functions, as well as its anti-hair loss and oil-control effects. When the mass ratio of lemon balm extract and gorgonian coral extract to the mass ratio of cypress leaf extract and black willow bark extract is further selected to be (1.1-8):(0.6-2.5), especially (2.3-6.7):(0.9-1.9), the resulting male anti-hair loss composition exhibits superior oil control, anti-hair loss, anti-inflammatory, antipruritic, soothing, and skin barrier repair functions. When the mass ratio of lemon balm extract and gorgonian coral extract to the mass ratio of cypress leaf extract and black willow bark extract is 4.5:1.3, the overall comprehensive effect of the composition is maximized.

[0015] In a preferred embodiment of the first aspect, the mass ratio of lemon balm extract to gorgonian coral extract in the composition is lemon balm extract:gorgonian coral extract = (1-7):(0.1-1). For example, the mass ratio of lemon balm extract to gorgonian coral extract can be, but is not limited to, 1:0.1, 2:0.2, 4:0.5, 5:0.6, 6:0.8, or 7:1.

[0016] In a preferred embodiment of the first aspect, the mass ratio of lemon balm extract to coral extract in the composition is lemon balm extract: coral extract = (2-6):(0.3-0.7).

[0017] In a preferred embodiment of the first aspect, the mass ratio of lemon balm extract to coral extract in the composition is lemon balm extract: coral extract = 3.8:0.7.

[0018] This invention has found that the mass ratio of lemon balm extract to gorgonian coral extract in the anti-hair loss and oil-controlling composition can, to a certain extent, affect the composition's TNF-α inhibitory capacity and the improvement effects of a* and TEWL values, thereby influencing the composition's anti-inflammatory and antipruritic effects, its ability to improve redness, and its efficacy in reducing oxidative stress in nerve cells, as well as its skin barrier repair and soothing effects. When the mass ratio of lemon balm extract to gorgonian coral extract is further selected to be (1-7):(0.1-1), especially (2-6):(0.3-0.7), the composition's anti-inflammatory effect on the skin and its effect on oxidative stress in nerve cells can be enhanced, as well as its ability to improve mood and its skin barrier repair and soothing capabilities can be strengthened. When the mass ratio of lemon balm extract to gorgonian coral extract is 3.8:0.7, the overall comprehensive effect of the composition can be maximized.

[0019] In a preferred embodiment of the first aspect, the mass ratio of cypress leaf extract to black willow bark extract in the composition is cypress leaf extract: black willow bark extract = (0.5-2):(0.1-0.5). For example, the mass ratio of cypress leaf extract to black willow bark extract is 0.5:0.1, 0.8:0.2, 1:0.2, 1.5:0.3, or 2:0.5.

[0020] In a preferred embodiment of the first aspect, the mass ratio of the cypress leaf extract to the black willow bark extract in the composition is cypress leaf extract: black willow bark extract = (0.7-1.5):(0.2-0.4).

[0021] In a preferred embodiment of the first aspect, the mass ratio of cypress leaf extract to black willow bark extract in the composition is cypress leaf extract: black willow bark extract = 0.9:0.4.

[0022] This invention has found that the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract in the anti-hair loss and oil-controlling composition can, to a certain extent, affect the composition's inhibitory effect on TNF-α, its a* value, and its TEWL value improvement. This, in turn, affects the composition's anti-inflammatory, soothing, and skin barrier repair effects, as well as its ability to increase dermal papilla cells and reduce sebum secretion. Further optimization of the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract to (0.5-2):(0.1-0.5), especially (0.7-1.5):(0.2-0.4), can enhance the composition's soothing and skin barrier repair capabilities, and also improve its anti-inflammatory, anti-hair loss, and oil-controlling effects. When the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract is 0.9:0.4, the overall comprehensive effect of the composition can be maximized.

[0023] As a preferred embodiment of the first aspect, the method for preparing the lemon balm extract includes the following steps: drying lemon balm flowers, pulverizing them, adding them to a phosphate buffer solution for enzymatic hydrolysis, collecting the hydrolysis product, centrifuging, filtering, and the filtrate is the lemon balm extract; wherein, the enzymatic hydrolysis uses cellulase.

[0024] As a preferred embodiment of the first aspect, at least one of the following is satisfied:

[0025] (a) The mass ratio of the phosphate buffer to lemon balm powder is (8-10):1; or / and the pH of the phosphate buffer is 4.5-5;

[0026] (b) The mass ratio of the cellulase to lemon balm powder is (1-1.5):100;

[0027] (c) The enzymatic hydrolysis time is 10-30 min; or / and the enzymatic hydrolysis temperature is 45-55℃.

[0028] This invention has found that different extraction methods affect lemon balm extract and its synergistic effect with other components, thus affecting the inhibition rate of TNF-α and the improvement of a* and TEWL values ​​of the composition. Consequently, it affects the composition's anti-inflammatory and antipruritic effects, its ability to improve redness and reduce oxidative stress in nerve cells, as well as its skin barrier repair and soothing effects, thereby affecting the overall comprehensive effect of the composition.

[0029] In a second aspect, the present invention provides the use of the male anti-hair loss composition as described in the first aspect in the preparation of anti-hair loss products.

[0030] Thirdly, the present invention provides a men's anti-hair loss product, the men's anti-hair loss product comprising the men's anti-hair loss composition described in the first aspect.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] This invention discovers that lemon balm extract, willow coral extract, cypress leaf extract, and black willow bark extract have a synergistic effect among numerous plant extracts. A specific formulation of these extracts can improve hair loss problems in sensitive skin from multiple dimensions, including comprehensive mood regulation, activation of autophagy, oil control, and soothing. This anti-hair loss composition combines physiological anti-hair loss ingredients with psychological mood-soothing ingredients, achieving a dual intervention for hair loss problems. Furthermore, all ingredients are plant-based, making it more suitable for Asian men with sensitive skin. Detailed Implementation

[0033] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below with reference to specific embodiments. The purpose is to provide a detailed understanding of the invention, not to limit it. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention. Unless otherwise specified, the experimental reagents and instruments designed in the embodiments and comparative examples of this invention are commonly used reagents and instruments, all of which are commercially available. Unless otherwise specified, the experimental methods used in the embodiments and comparative examples are conventional methods; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch. Further explanation of the raw materials used in this invention:

[0034] The cypress leaf extract was purchased from Xi'an Youbohui Biotechnology Co., Ltd., product name: Cypress (CUPREESSUS FUNEBRIS) leaf extract, item number: 111111;

[0035] The black willow bark extract was purchased from Xi'an Lishi Biotechnology Co., Ltd., product name: black willow bark extract, product number: LS-PG;

[0036] The gorgonian coral extract was purchased from Lanzhou Waterles Biotechnology Co., Ltd. Product name: Gorgonian coral extract;

[0037] Grape seed extract replaced lemon balm extract. Purchased from Shaanxi Yinuo Biotechnology Co., Ltd. Product name: grape seed extract, item number: 5554.

[0038] The extract of Platycladus orientalis leaf replaced the extract of Platycladus orientalis leaf, purchased from Shanxi Yirun Biotechnology Co., Ltd., product name: Platycladus orientalis leaf extract;

[0039] White willow bark extract replaced black willow bark extract. It was purchased from Xi'an Lishi Biotechnology Co., Ltd. Product name: white willow bark extract, product number: LS-blp.

[0040] Hawthorn leaf extract replaced coral extract. It was purchased from Shaanxi Lvshengyuan Biological Products Manufacturing Co., Ltd. Product name: Hawthorn leaf extract, item number: SXLSYSZYTQW.

[0041] The preparation method of lemon balm extract-1 is as follows:

[0042] Commercially available lemon balm flowers were dried, pulverized, and passed through a 40-mesh sieve. They were then mixed with 8 times their weight of phosphate-buffered saline (PBS) to adjust the pH to 4.5. Cellulase was added, and the mixture was stirred at 45°C for enzymatic hydrolysis. After that, the mixture was placed at 90°C for 10 minutes to inactivate the enzyme. The mixture was then centrifuged and filtered. The filtrate was lemon balm extract-1. The mass ratio of cellulase to lemon balm powder was 1:100.

[0043] The preparation method of lemon balm extract-2 is as follows:

[0044] Commercially available lemon balm flowers were dried, pulverized, and passed through a 50-mesh sieve. 9 kg of phosphate-buffered saline (PBS) was added and mixed to adjust the pH to 4.5. Cellulase was added, and the mixture was stirred at 50°C for enzymatic hydrolysis. The mixture was then placed at 90°C for 20 min to inactivate the enzyme. After centrifugation and filtration, the filtrate was obtained as lemon balm extract-2. The mass ratio of cellulase to lemon balm powder was 1.2:100.

[0045] The preparation method of lemon balm extract-3 is as follows:

[0046] Commercially available lemon balm flowers were dried, pulverized, and passed through a 60-mesh sieve. 10 liters of phosphate-buffered saline (PBS) were added and mixed to adjust the pH to 5.0. Cellulase was added and the mixture was stirred at 50°C for enzymatic hydrolysis. After that, the mixture was placed at 90°C for 30 minutes to inactivate the enzyme. Then, the mixture was centrifuged and filtered. The filtrate was lemon balm flower extract-3. The mass ratio of cellulase to lemon balm flower powder was 1.5:100.

[0047] The preparation method of lemon balm extract-4 is as follows:

[0048] Commercially available lemon balm was dried, pulverized, and passed through a 40-mesh sieve. 1 g of lemon balm powder was added to 20 mL of 70% ethanol and ultrasonically extracted at 40°C for 30 min with an ultrasonic extraction power of 120 W. The product was centrifuged and the supernatant was concentrated to 5 mL to obtain lemon balm extract-4.

[0049] The preparation method of lemon balm extract-5 is as follows:

[0050] Take commercially available lemon balm, dry and pulverize it, and pass it through a 40-mesh sieve. Take 1g of lemon balm powder, add 20mL of 70% ethanol, and reflux at 80℃ for 1h to obtain lemon balm extract-5.

[0051] The preparation method of the anti-hair loss and oil control composition suitable for Asian men with sensitive skin of the present invention is as follows: weigh the components according to the weight parts shown in Table 1, add water to 20g, stir and mix evenly, and sonicate for 10 minutes to obtain the anti-hair loss and oil control composition.

[0052] Examples and Comparative Examples

[0053] This application provides an anti-hair loss and oil control composition suitable for sensitive skin of Asian men. The components and weight parts of the composition are shown in Table 1. The comparative examples are based on Example 1. For comparative examples 3-6, if any component is missing, the total amount is the same as that in Example 1.

[0054] Table 1:

[0055]

[0056] Example of effect

[0057] This example demonstrates the anti-inflammatory ability of the composition of the present invention using cell experiments, as detailed below:

[0058] Experimental methods:

[0059] Plating: Logarithmically growing RAW264.7 cells were seeded into 24-well plates at a cell density of 2 × 10⁻⁶ cells / well. 5 Cells / mL, 0.5mL per well, after plating the plate, mark the top cover of the cell plate and incubate in a CO2 incubator for 24 h;

[0060] Sample preparation: After cell incubation for 24 h, the supernatant was aspirated, and 0.5 mL of the following samples were added respectively: ① Blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ Positive control group: 0.001% dexamethasone + LPS (1 μg / mL); ④ Experimental group: 1% of the example or comparative composition + LPS (1 μg / mL).

[0061] Detection of inflammatory factors in cell supernatant: After cell treatment for 24 h, the supernatant was pipetted into centrifuge tubes, centrifuged at 1000 r / min for 5 min, the precipitate was discarded, and the supernatant was collected. The changes in inflammatory factors in the supernatant were detected using the Xinbosheng TNF-α inflammatory factor detection kit. The inhibition rate of TNF-α was calculated as follows: TNF-α inhibition rate % = (TNF-α amount in model group - TNF-α amount in experimental group) / TNF-α amount in model group * 100%; where a higher inhibition rate indicates a stronger anti-inflammatory ability. The calculated results are shown in Table 2.

[0062] Table 2:

[0063]

[0064] As can be seen from Table 1, the male anti-hair loss and oil-control composition provided by the present invention can significantly improve the TNF-α inhibition rate, which indicates that the composition of the present invention has a strong anti-inflammatory effect.

[0065] As can be seen from Examples 1-14 and Comparative Examples 1-2, the mass ratio of the components in the anti-hair loss and oil control composition affects the composition's ability to inhibit TNF-α, thereby affecting the overall anti-inflammatory efficacy of the composition.

[0066] Examples 1-7 show that the mass ratio of the components in the composition affects the composition's ability to inhibit TNF-α. When the mass ratio of lemon balm extract to coral extract to cypress leaf extract to black willow bark extract is limited to the range of (1.1-8):(0.6-2.5), the resulting composition exhibits strong anti-inflammatory effects, especially when the mass ratio is in the range of (2.3-6.7):(0.9-1.9), the anti-inflammatory effect is even better, and the effect is optimal when the mass ratio is 4.5:1.3 (Example 7).

[0067] This invention investigated the effect of the mass ratio of lemon balm extract to gorgonian coral extract on the inhibition of TNF-α in the composition through Examples 8-10. Examples 8-10 show that the mass ratio of lemon balm extract to gorgonian coral extract affects the composition's inhibitory effect on TNF-α, thus influencing its anti-inflammatory efficacy. When the mass ratio of lemon balm extract to gorgonian coral extract is limited to the range of (1-7):(0.1-1), the TNF-α inhibitory ability of the composition is improved, especially when the range is (2-6):(0.3-0.7), the composition shows even better results. The optimal effect is achieved when the mass ratio is 3.8:0.7 (Example 9).

[0068] This invention investigated the effect of the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract on the inhibition of TNF-α in the composition through Examples 11-13. Examples 11-13 show that the mass ratio affects the composition's inhibitory effect on TNF-α, thus influencing its anti-inflammatory efficacy. When the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract is limited to the range of (0.5-2):(0.1-0.5), the TNF-α inhibitory ability of the composition is improved, especially when the ratio is in the range of (0.7-1.5):(0.2-0.4), the composition shows even better results. The optimal effect is achieved when the mass ratio is 0.9:0.4 (Example 12).

[0069] In summary, the optimal ratio of each component in the composition of the present invention is: lemon balm extract: coral bark extract: cypress leaf extract: black willow bark extract = 3.8:0.7:0.9:0.4 (Example 14).

[0070] This invention investigated the effects of lemon balm extracts obtained by different extraction methods on the ability of the composition to inhibit TNF-α through Examples 15-18. Examples 15-18 show that the extraction method of the lemon balm extract has a certain impact on the TNF-α inhibition effect, thus affecting the anti-inflammatory efficacy of the composition. The composition obtained using lemon balm extract-2 showed the best anti-inflammatory effect (Example 15).

[0071] The TNF-α inhibition rate of Comparative Examples 1-2 was 26%. This is because the mass ratio of each component in the compositions of Comparative Examples 1-2 was not within the scope of this invention, which affected the synergistic effect between the components and reduced the efficacy of the composition.

[0072] The TNF-α inhibition rate of Comparative Examples 3-6 was 22-23%, which is because the absence of one of the components in this invention in the compositions of Comparative Examples 3-6 affected the synergistic effect between the components and reduced the efficacy of the composition.

[0073] The TNF-α inhibition rate of Comparative Examples 7-10 was 24-25%. This is because the compositions of Comparative Examples 7-10 replaced one of the components in the composition of the present invention with other plant extracts, which did not produce a synergistic effect with the specific components selected in the present invention, and also affected the synergistic effect between other components of the present invention, thus reducing the efficacy of the composition.

[0074] Application examples

[0075] This invention provides a men's anti-hair loss product in application examples and comparative application examples. The components (mass percentage) of the anti-hair loss product are shown in Table 3. In application examples 1-18, the anti-hair loss and oil-controlling compositions used are the anti-hair loss and oil-controlling compositions prepared in examples 1-18, respectively. In application examples 19-20, the composition prepared in example 15 is used. In comparative application examples 1-10, the anti-hair loss and oil-controlling compositions used are the anti-hair loss and oil-controlling compositions prepared in comparative examples 1-10, respectively. A blank application example is also provided, the only difference between the blank application example and application example 1 being that no anti-hair loss and oil-controlling composition is added.

[0076] Table 3:

[0077]

[0078] This embodiment provides a method for preparing shampoo, the method comprising the following steps:

[0079] (1) Add polyquaternium-10 to deionized water and stir to disperse. After the dispersion is uniform, add other materials of phase A, heat to 85°C, stir to dissolve evenly, and then keep warm for later use to obtain pre-phase A;

[0080] (2) Add each component of phase B at 80-85℃, stir thoroughly to dissolve evenly until transparent, and keep warm to defoam;

[0081] (3) Cool down to below 45°C, add the C phase raw materials one by one, and stir evenly;

[0082] (4) Continue to add phase D raw materials and mix evenly to obtain men's anti-hair loss shampoo.

[0083] Test Example 1: Testing the improvement of TEWL and a* values ​​of anti-hair loss products

[0084] Several male volunteers aged 18-45 were selected. On the day of the visit, the subjects sat quietly for 30 minutes in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10%. The initial a* value and initial TEWL value at the hairline were measured using the Skin-Colorimeter CL-400 skin color probe and the Tewameter®™ Hex (Courage+Khazaka) skin transepidermal water loss probe. The subjects were randomly divided into 31 groups of 5 people each, and randomly used the anti-hair loss product provided by this invention. Various indicators were tested at 8 weeks and 12 weeks, and the improvement of the skin TEWL value and a* value of each volunteer at 8 weeks and 12 weeks was analyzed. The improvement was expressed as the improvement rate of each value, and the calculation method is as follows:

[0085] Improvement rate relative to the initial value = |(X 使用后 -X 使用前 ) / X 使用前 ×100%|; where the TEWL value represents the skin barrier repair effect, and the higher the improvement rate, the stronger the skin barrier repair ability; the a* value represents the soothing function, and the higher the improvement rate, the stronger the soothing ability. The calculated data are shown in Table 4;

[0086] Table 4:

[0087]

[0088] As can be seen from Table 4, the shampoo provided by this invention has excellent soothing and skin barrier repair effects. Specifically, at week 12, the a* improvement rate was above 11%, and the TEWL value improvement rate was above 13%.

[0089] As can be seen from Application Examples 1-14 and Comparative Examples 1-2, the mass ratio of the components in the men's anti-hair loss and oil-control composition affects the composition's ability to improve TEWL and a* values, thereby affecting the overall soothing and skin barrier repair efficacy of the composition.

[0090] As can be seen from Application Examples 1-7, the mass ratio of the components in the composition affects the composition's ability to soothe and repair the skin barrier. When the mass ratio of lemon balm extract and coral extract to that of cypress leaf extract and black willow bark extract is limited to the range of (1.1-8):(0.6-2.5), the resulting composition has a strong soothing and skin barrier repairing effect, especially when the mass ratio is in the range of (2.3-6.7):(0.9-1.9), the composition has a better soothing and skin barrier repairing effect. The best effect is achieved when the mass ratio is 4.5:1.3 (Application Example 7).

[0091] This invention investigated the effect of the mass ratio of lemon balm extract to gorgonian coral extract in the composition on improving the TEWL value through Application Examples 8-10. Application Examples 8-10 show that the mass ratio of lemon balm extract to gorgonian coral extract affects the composition's ability to improve the TEWL value, thus influencing its barrier repair efficacy. When the mass ratio of lemon balm extract to gorgonian coral extract is limited to the range of (1-7):(0.1-1), the composition's ability to improve the TEWL value is enhanced, especially at (2-6):(0.3-0.7), where the composition shows even better results. The optimal effect is achieved when the mass ratio is 3.8:0.7 (Application Example 9).

[0092] This invention investigated the effect of the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract on the improvement of the a* value of the composition through Application Examples 11-13. Application Examples 11-13 show that the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract affects the composition's effect on improving the a* value, thus influencing the composition's soothing efficacy. When the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract is limited to the range of (0.5-2):(0.1-0.5), the soothing ability of the composition is improved, especially at (0.7-1.5):(0.2-0.4), the composition exhibits even better efficacy. The optimal effect is achieved when the mass ratio is 0.9:0.4 (Application 12).

[0093] In summary, based on the above results, the optimal combination of the present invention is: the mass ratio of lemon balm extract, coral bark extract, cypress leaf extract, and black willow bark extract is 3.8:0.7:0.9:0.4 (Application Example 14).

[0094] This invention investigated the effects of lemon balm extracts obtained by different extraction methods on the soothing and barrier repair capabilities of the compositions through Application Examples 15-18. It can be seen that the extraction method of lemon balm extract affects the improvement of TEWL values, thereby affecting the barrier repair effect. The compositions obtained using lemon balm extract-2 showed the best results (Application Example 15).

[0095] Compared with Application Examples 1-2, the TEWL value improvement rate was 12% and the a* value improvement was 10%. This is because the mass ratio of each component in the composition of Application Examples 1-2 was not within the range of the present invention, which affected the synergistic effect between the components and reduced the efficacy of the composition.

[0096] The improvement rate of TEWL value in comparative application examples 3-6 was 11%, and the improvement of a* value was 9%. This is because the absence of one of the components of the present invention in the composition of comparative application examples 3-6 affected the synergistic effect between components and reduced the efficacy of the composition.

[0097] The improvement rate of TEWL value in comparative application examples 7-10 was 11-12%, and the improvement of a* value was 9-10%. This is because the composition of comparative application examples 7-10 replaced one of the components in the composition of the present invention with other plant extracts, which did not produce a synergistic effect with the specific components selected in the present invention, and also affected the synergistic effect between other components of the present invention, thus reducing the efficacy of the composition.

[0098] Test Example 2: Test on the anti-hair loss effect and its impact on mood of anti-hair loss products.

[0099] Several male volunteers aged 18-45 with varying degrees of hair loss were selected and divided into 31 groups of 5 people each. Participants used the hair loss prevention product (either the example or control product) once daily. Hair loss was counted after 8 and 12 weeks of use, using the 60-comb test to record the number of hairs lost during each combing. The number of hairs lost before product use and after 8 and 12 weeks of use was recorded. Participants did not wash their hair for 48 ± 4 hours before each visit and did not comb their hair on the day of the visit. The hair loss reduction rate was calculated as follows: Hair loss reduction rate / % = (Number of hairs lost at week 0 - Number of hairs lost at weeks 8 / 12) / Number of hairs lost at week 0. The calculated data are shown in Table 5.

[0100] In addition, volunteers completed the Self-Assessment Manikin (SAM) scale to evaluate their current skin sensations. The assessment used a 9-point scale, with higher scores indicating greater pleasure. Subjects rated their skin before and after the first use, and the results were expressed as an improvement rate: Improvement Rate / % = (Post-use score - Post-use score) / Pre-use score. The evaluation results are shown in Table 5.

[0101] Table 5

[0102]

[0103] As shown in Table 5, the shampoo provided by this invention has excellent anti-hair loss and mood-improving effects. Specifically, at week 12, the hair loss reduction rate was over 40%.

[0104] As can be seen from Application Examples 1-14 and Comparative Examples 1-2, the mass ratio of the components in the men's anti-hair loss composition affects the anti-hair loss effect of the composition.

[0105] As can be seen from Application Examples 1-7, the mass ratio of the components in the composition affects the anti-hair loss effect of the composition. When the mass ratio of lemon balm extract to coral extract to cypress leaf extract to black willow bark extract is limited to the range of (1.1-8):(0.6-2.5), the resulting composition has a strong anti-hair loss effect, especially when it is (2.3-6.7):(0.9-1.9), the anti-hair loss effect of the resulting composition is even better, and the effect is best when the mass ratio is 4.5:1.3 (Application Example 7).

[0106] This invention investigated the effect of the mass ratio of lemon balm extract to gorgonian coral extract on the anti-hair loss effect of the composition through Application Examples 8-10. Application Examples 8-10 show that the mass ratio of lemon balm extract to gorgonian coral extract affects the anti-hair loss effect of the composition. When the mass ratio of lemon balm extract to gorgonian coral extract is limited to the range of (1-7):(0.1-1), the anti-hair loss effect of the composition is improved, especially when it is (2-6):(0.3-0.7), the effect of the resulting composition is even better. The optimal effect is achieved when the mass ratio is 3.8:0.7 (Application Example 9).

[0107] This invention investigated the effect of the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract on the anti-hair loss effect of the composition through Application Examples 11-13. Application Examples 11-13 show that the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract affects the anti-hair loss effect of the composition. When the mass ratio of *Platycladus orientalis* leaf extract to *Salix matsudana* bark extract is limited to the range of (0.5-2):(0.1-0.5), the ability of the composition to improve the a* value is enhanced, especially at (0.7-1.5):(0.2-0.4), the effect of the resulting composition is even better. The optimal effect is achieved when the mass ratio is 0.9:0.4 (Application 12).

[0108] In summary, based on the above results, the optimal combination of the present invention is: the mass ratio of lemon balm extract, coral bark extract, cypress leaf extract, and black willow bark extract is 3.8:0.7:0.9:0.4 (Application Example 14).

[0109] This invention investigated the effect of lemon balm extracts obtained by different extraction methods on the anti-hair loss effect of the compositions through Application Examples 15-18. It can be seen that the extraction method of lemon balm extract has a certain impact on the anti-hair loss effect. The compositions obtained using lemon balm extract-2 showed the best effect (Application Example 15).

[0110] The hair loss reduction rate of comparative application examples 1-2 was 35-36%. This is because the mass ratio of each component in the composition of comparative application examples 1-2 was not within the scope of the present invention, which affected the synergistic effect between the components and reduced the efficacy of the composition.

[0111] The hair loss reduction rate of comparative application examples 3-6 was 27-30%. This is because the absence of one of the components of the present invention in the composition of comparative application examples 3-6 affected the synergistic effect between the components and reduced the efficacy of the composition.

[0112] The hair loss reduction rate of comparative application examples 7-10 was 32-34%. This is because the composition of comparative application examples 7-10 replaced one of the components in the composition of the present invention with other plant extracts, which did not produce a synergistic effect with the specific components selected in the present invention, and also affected the synergistic effect between other components of the present invention, thus reducing the efficacy of the composition.

[0113] Test Example 3: Consumer Use Test of the Anti-Hair Loss Product of This Invention for Asian Men with Sensitive Skin

[0114] This test case verifies the suitability and soothing effect of the product prepared in Example 1 for Asian male consumers with sensitive skin, specifically including the following steps:

[0115] Thirty male subjects with sensitive facial skin and recent symptoms of sensitive skin were selected for the test. They were given the product and each subject applied an appropriate amount to the corresponding area daily for a 28-day trial to assess its suitability for sensitive skin. Consumers completed a questionnaire survey on the degree of improvement in indicators such as anti-hair loss effect, soothing effect, oil control effect, and irritation. See Table 6 for details.

[0116] Table 6:

[0117]

[0118] As can be seen from Table 6, the anti-hair loss product of the present invention, which incorporates an anti-hair loss and oil-controlling composition, has the effects of preventing hair loss, soothing, and relieving itching. It can provide the test subjects with a refreshing and comfortable feeling. At the same time, the anti-hair loss product of the present invention is gentle and non-irritating, and is suitable for people with sensitive skin.

[0119] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A hair loss prevention and oil control composition suitable for Asian men, characterized in that, The composition comprises the following components in parts by weight: 0.5-10 parts lemon balm extract, 0.05-3 parts coral sage extract, 0.02-3 parts cypress leaf extract, and 0.05-0.8 parts black willow bark extract; The preparation method of the lemon balm extract includes the following steps: After drying the flowers of lemon balm, they are crushed and mixed with phosphate buffer for enzymatic hydrolysis. The hydrolysate is collected, centrifuged, and filtered. The filtrate is the lemon balm extract. Cellulase is used for the enzymatic hydrolysis. The preparation method of the lemon balm extract meets the following conditions: (a) The mass ratio of the phosphate buffer to lemon balm powder is (8-10):1; (b) The pH value of the phosphate buffer solution is 4.5-5; (b) The mass ratio of the cellulase to lemon balm powder is (1-1.5):100; (c) The enzymatic hydrolysis time is 10-30 min; the enzymatic hydrolysis temperature is 45-55℃.

2. The anti-loosening and oil-controlling composition according to claim 1, characterized in that, The mass ratio of the sum of lemon balm extract and coral extract to the sum of cypress leaf extract and black willow bark extract in the composition is: (lemon balm extract + coral extract) : (cypress leaf extract + black willow bark extract) = (1.1-8) : (0.6-2.5).

3. The anti-loosening and oil-controlling composition as described in claim 2, characterized in that, The mass ratio of the sum of lemon balm extract and coral extract to the sum of cypress leaf extract and black willow bark extract in the composition is: (lemon balm extract + coral extract) : (cypress leaf extract + black willow bark extract) = (2.3-6.7) : (0.9-1.9).

4. The anti-loosening and oil-controlling composition according to claim 1, characterized in that, In the composition, the mass ratio of lemon balm extract to coral extract is: lemon balm extract: coral extract = (1-7):(0.1-1).

5. The anti-loosening and oil-controlling composition according to claim 4, characterized in that, In the composition, the mass ratio of lemon balm extract to coral extract is: lemon balm extract: coral extract = (2-6):(0.3-0.7).

6. The anti-loosening and oil-controlling composition according to claim 1, characterized in that, In the composition, the mass ratio of cypress leaf extract to black willow bark extract is: cypress leaf extract : black willow bark extract = (0.5-2) : (0.1-0.5).

7. The anti-loosening and oil-controlling composition according to claim 6, characterized in that, In the composition, the mass ratio of cypress leaf extract to black willow bark extract is: cypress leaf extract : black willow bark extract = (0.7-1.5) : (0.2-0.4).

8. The use of the anti-hair loss and oil control composition according to any one of claims 1-7 in the preparation of anti-hair loss products.

9. A men's anti-hair loss product, characterized in that, The men's anti-hair loss product includes the anti-hair loss and oil-control composition as described in any one of claims 1-7.

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