Targeted bacteriostatic acne-removing composition and application thereof

Abstract

The invention provides an acne-removing composition capable of achieving targeted bacteriostasis and application of the acne-removing composition. The acne-removing composition capable of achieving targeted bacteriostasis is prepared from the following components in parts by weight: 0.1 to 10 parts of biological super protein, 0.5 to 20 parts of plant extract and 0.1 to 5 parts of lactoglobulin, the plant extract comprises a white willow bark extract, a rhodomyrtus tomentosa extract and a pansy extract. According to the acne-removing composition capable of inhibiting bacteria in a targeted manner, the biological super-protein, the plant extract and the lactoglobulin are matched with one another, so that the acne-removing composition has a synergistic effect in the aspects of inhibiting bacteria and removing acnes, and can better inhibit inflammatory reaction in a multi-link manner, eliminate free radicals and reduce grease secretion, thereby achieving an all-around rapid acne-removing effect; the obvious acne removing effect can be achieved within 24 hours.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and particularly relates to an anti-acne composition capable of targeted antibacterial properties and an application thereof. Background Art

[0002] Acne, also known as pimples, is a chronic inflammatory disease of the hair follicle sebaceous gland unit, with clinical manifestations of acne, papules, nodules, cysts and even scars. Acne often occurs on the face, chest and back, seriously affecting the health and beauty of human skin, thereby affecting people's psychology, work and life. At present, the pathogenesis of acne has not been fully studied, but it is generally recognized that there are four causes: (1) abnormal regulation of sebaceous glands by androgens; (2) abnormal keratinization of sebaceous gland ducts; (3) excessive reproduction of Propionibacterium acnes; (4) verification of immune response. Based on the pathogenesis of acne, there are already a large number of products for acne removal on the market.

[0003] CN114344230A discloses a composition for inhibiting bacteria, removing mites and removing acne, its preparation method and its application, which belongs to the field of daily cosmetics. It is made of the following raw materials: 6-8 parts of betaine hydrochloride, 12-15 parts of leucine methyl ester hydrochloride, 9-12 parts of methionine ethyl ester hydrochloride, 8-10 parts of thickener, 15-18 parts of glycerol, 4-6 parts of pH regulator, 18-20 parts of benzoyl peroxide, 27-30 parts of herbal extract, and 38-45 parts of water. The composition for inhibiting bacteria, removing mites and removing acne of the application can be added and applied as a variety of different types of daily cosmetics, and the obtained daily cosmetics have skin care effects such as antibacterial, removing mites, removing acne and anti-inflammatory, and the effect is mild, which is conducive to the repair of the skin barrier and restores the skin to health.

[0004] CN114344239A discloses an anti-acne composition and a preparation method thereof. The raw materials for the preparation thereof include, by weight: 1-5 parts of a synthetic compound I, 0.5-5 parts of a nutrient solution, 0.001-0.01 parts of pionin, 0.1-1.5 parts of a zinc-containing substance, 0.2-2 parts of a synthetic compound II, and 0.1-1.5 parts of a synthetic compound III. The anti-acne composition prepared by the present invention can effectively improve the balance of bacterial flora on the skin, thereby transforming acne-prone skin into healthy skin, fundamentally solving the serious acne problem on the face of teenagers and solving the troubles of teenagers, and has high practical value and promotion value.

[0005] However, most of the existing acne-removing products mentioned above use chemical antibacterial agents to improve acne. The use of chemical ingredients can easily cause skin irritation, sensitivity, and thinning. Long-term use may damage the epidermal barrier; and it will inhibit bacteria that are beneficial to the skin, thereby causing the microbial balance to be destroyed. Therefore, there is an urgent need for a product that is mild, non-irritating, and has a better acne-removing effect to meet people's needs. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide an acne-removing composition with targeted antibacterial properties and its application. The acne-removing composition with targeted antibacterial properties provided by the present invention has mild performance and avoids the problems of irritation, drug resistance and destruction of skin microecological balance caused by the use of chemical antibacterial agents, antibiotics, broad-spectrum bactericides, etc.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides an anti-acne composition capable of targeted antibacterial activity, wherein the anti-acne composition capable of targeted antibacterial activity comprises, by weight, 0.1-10 parts of biological super protein, 0.5-20 parts of plant extract, and 0.1-5 parts of lactoglobulin;

[0009] The plant extracts include white willow bark extract, myrtle extract and pansy extract.

[0010] The biological super protein in the targeted antibacterial acne removal composition of the present invention has stable performance and excellent antibacterial activity; the plant extract has anti-inflammatory and antibacterial effects, and can improve the anti-inflammatory and acne removal effect of the composition; the lactoglobulin can resist the invasion of foreign matter on the organism, and can be combined with androgen to make it inactive, thereby reducing sebum secretion. The combination of the three has a synergistic effect in antibacterial and acne removal, can better multi-link inhibit inflammatory response, eliminate free radicals and reduce oil secretion, thereby achieving a comprehensive and rapid acne removal effect.

[0011] In addition, the white willow bark extract in the plant composition of the present invention has excellent antioxidant and pore-tightening effects, can inhibit sebum secretion, and the salicin contained in it can replace chemical antibacterial ingredients to play a mild exfoliating effect; the pansy extract can scavenge oxygen free radicals and inhibit the inflammatory response caused by sebum oxidation; the myrtle extract can reduce lipid synthesis by inhibiting 5а-reductase, can restore the balance between Propionibacterium acnes strains (pathogenic and non-pathogenic types), and inhibit inflammation caused by Propionibacterium acnes and inflammation induced by squalene peroxide; the plant extract uses the above-mentioned specific components, so that the composition can achieve the effect of mild pore dredging and targeted acne removal, and has a significant acne removal effect within 24 hours.

[0012] The amount of the biological super protein added in the targeted antibacterial acne-removing composition of the present invention can be 0.5 parts, 0.8 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0013] The added amount of the plant extract can be 0.8 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts or 19 parts, etc.;

[0014] The added amount of the lactoglobulin can be 0.3 parts, 0.5 parts, 0.8 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.

[0015] Preferably, the anti-acne composition capable of targeted antibacterial activity comprises, by weight, 1-8 parts of biological super protein, 3-15 parts of plant extract and 0.1-3 parts of lactoglobulin.

[0016] Preferably, the anti-acne composition capable of targeted antibacterial activity comprises, by weight, 1-5 parts of biological super protein, 5-12 parts of plant extract and 0.1-1 part of lactoglobulin.

[0017] Preferably, the biological super protein is a mixture of bacteriophage lytic enzyme and collagen.

[0018] Preferably, the mass ratio of the willow bark extract, myrtle extract and pansy extract is (1-4):(0.5-2):(0.5-3).

[0019] Wherein "1-4" can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5 or 3.8, etc.;

[0020] “0.5-2” can be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0021] “0.5-3” can mean 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6 or 3.8, etc.

[0022] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0023] In a second aspect, the present invention provides a method for preparing the anti-acne composition with targeted antibacterial properties as described in the first aspect, the preparation method comprising: mixing biological super protein, plant extract and lactoglobulin according to the formula amount, and obtaining the anti-acne composition with targeted antibacterial properties after uniform mixing.

[0024] In a third aspect, the present invention provides a use of the anti-acne composition capable of targeted antibacterial properties as described in the first aspect in an anti-acne product.

[0025] Preferably, the acne-removing product comprises an acne-removing essence, an acne-removing cream or an acne-removing facial cleanser.

[0026] Preferably, the mass percentage of the targetable antibacterial acne-removing composition in the acne-removing product is 1-20%, for example, it can be 1%, 3%, 5%, 7%, 9%, 10%, 11%, 13%, 15%, 17% or 19%, etc. Specific point values ​​within the above numerical range can be selected and will not be described one by one here.

[0027] In a fourth aspect, the present invention provides an anti-acne essence, comprising the anti-acne composition with targeted antibacterial properties as described in the first aspect, an emulsifier, a moisturizer, a thickener and deionized water.

[0028] The acne-removing essence of the present invention adds an acne-removing composition with targeted antibacterial properties to the components, which can condition the skin and play an antibacterial and anti-inflammatory role as well as remove acne. Moreover, by adding components such as moisturizers and emulsifiers, the skin can be improved from the inside out, whitening the skin and maintaining the moisture content of the skin, thereby meeting the different care needs of beauty lovers.

[0029] Preferably, the anti-acne essence comprises, by mass percentage, 1-20% of the anti-acne composition capable of targeted antibacterial activity, 1-10% of an emulsifier, 1-20% of a moisturizer, 0.1-5% of a thickener, and 10-90% of deionized water.

[0030] The mass percentage of the targeted antibacterial anti-acne composition in the anti-acne essence of the present invention may be 1%, 3%, 5%, 7%, 9%, 10%, 11%, 13%, 15%, 17% or 19%, etc.;

[0031] The mass percentage of the emulsifier can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8% or 9%;

[0032] The mass percentage of the moisturizing agent can be 1%, 3%, 5%, 7%, 9%, 10%, 11%, 13%, 15%, 17% or 19%, etc.;

[0033] The mass percentage of the thickener can be 0.3%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4% or 4.5%, etc.;

[0034] The mass percentage of the deionized water can be 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95%, etc.

[0035] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0036] Preferably, the emulsifier is any one of steareth-2, steareth-21, glyceryl stearate, polysorbate-60, cetearyl glucoside, C12-20 alkyl glucoside, PEG-6 caprylic / capric glycerides or capryl / caprylamidopropyl betaine, or a combination of at least two thereof.

[0037] Preferably, the thickener comprises any one of xanthan gum, carbomer or hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer or a combination of at least two thereof.

[0038] Preferably, the moisturizing agent includes any one of erythritol, panthenol, squalane, niacinamide, glycerin or 1,3-butylene glycol, or a combination of at least two thereof.

[0039] Preferably, the moisturizing agent is a combination of erythritol, panthenol, squalane and niacinamide.

[0040] Preferably, the mass ratio of erythritol, panthenol, squalane and niacinamide is (0.1-1):(2-5):(1-3):(2-4).

[0041] Wherein “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0042] “2-5” can be 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0043] "1-3" can be 1.2, 1.4, 1.5, 1.6, 1.8, 2, 2.2, 2.4, 2.5, 2.6 or 2.8, etc.;

[0044] “2-4” can be 2.2, 2.4, 2.5, 2.6, 2.7, 2.8, 3, 3.2, 3.4, 3.5, 3.6 or 3.8, etc.

[0045] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0046] Preferably, the anti-acne essence further comprises any one of a pH regulator, a preservative or a fragrance, or a combination of at least two of them.

[0047] In a fifth aspect, the present invention provides a method for preparing the anti-acne essence according to the third aspect, the preparation method comprising:

[0048] (1) Mixing a moisturizer and deionized water, heating the mixture, and then adding an emulsifier and a thickener and mixing until homogeneous;

[0049] (2) After cooling, the acne-removing composition with targeted antibacterial properties is added, mixed and allowed to stand to obtain the acne-removing essence.

[0050] Preferably, the temperature in step (1) is raised to 50-70°C, for example, 52°C, 54°C, 56°C, 58°C, 60°C, 62°C, 64°C, 66°C or 68°C.

[0051] Preferably, the temperature in step (2) is lowered to 25-35°C, for example, 26°C, 28°C, 30°C, 32°C or 34°C.

[0052] Preferably, the standing time in step (2) is 1-3 h, for example, it can be 1.2 h, 1.5 h, 1.8 h, 2 h, 2.2 h, 2.5 h or 2.8 h.

[0053] Preferably, step (2) further comprises adding any one or a combination of at least two of a pH adjuster, a preservative or a fragrance.

[0054] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0055] Compared with the prior art, the present invention has the following beneficial effects:

[0056] (1) The targeted antibacterial acne-removing composition of the present invention has a synergistic effect in antibacterial and acne-removing through the mutual cooperation of biological super protein, plant extract and lactoglobulin, and can better inhibit inflammatory response in multiple links, eliminate free radicals and reduce oil secretion, thereby achieving a comprehensive and rapid acne-removing effect, and has a significant acne-removing effect within 24 hours.

[0057] (2) The acne-removing essence of the present invention adds a targeted antibacterial acne-removing composition to the components, which can condition the skin and play an antibacterial and anti-inflammatory role as well as remove acne. Moreover, by adding moisturizers, emulsifiers and other components, the skin can be improved from the inside out, whitening the skin and maintaining the moisture content of the skin, thereby meeting the different care needs of beauty-conscious people. DETAILED DESCRIPTION

[0058] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0059] The following materials and comparative examples are as follows:

[0060] Raw material name Origin, manufacturer and model Biosuper protein Shenzhen Biotechnology Co., Ltd. White Willow Bark Extract Synsil Myrtle Extract Synsil Pansy Extract France Green Natural Personal Care Products Co., Ltd. Lactoglobulin Wuhan Heyuan Biotechnology Co., Ltd.

[0061] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0062] Example 1

[0063] This embodiment provides an anti-acne composition with targeted antibacterial properties, wherein the anti-acne composition with targeted antibacterial properties comprises, by weight, 5 parts of biological super protein, 5 parts of white willow bark extract, 2 parts of myrtle extract, 3 parts of pansy extract and 1 part of lactoglobulin;

[0064] The preparation method of the targeted antibacterial acne-removing composition is to mix biological super protein, white willow bark extract, myrtle extract, pansy extract and lactoglobulin according to the formula amount to obtain the targeted antibacterial acne-removing composition.

[0065] Example 2

[0066] This embodiment provides an anti-acne composition with targeted antibacterial properties, wherein the anti-acne composition with targeted antibacterial properties comprises, by weight, 1 part of biological super protein, 5 parts of white willow bark extract, 10 parts of myrtle extract, 5 parts of pansy extract, and 2 parts of lactoglobulin;

[0067] The preparation method of the targeted antibacterial acne-removing composition is as described in Example 1.

[0068] Example 3

[0069] This embodiment provides an anti-acne composition with targeted antibacterial properties, wherein the anti-acne composition with targeted antibacterial properties comprises, by weight, 8 parts of biological super protein, 2 parts of white willow bark extract, 0.5 parts of myrtle extract, 1.5 parts of pansy extract, and 1 part of lactoglobulin;

[0070] The preparation method of the targeted antibacterial acne-removing composition is as described in Example 1.

[0071] Example 4

[0072] This embodiment provides an anti-acne composition with targeted antibacterial properties. The only difference between the anti-acne composition with targeted antibacterial properties and that of Example 1 is that the plant extract does not include white willow bark extract, and the reduced amount thereof is distributed to the myrtle extract and the pansy extract in proportion to the number of parts. The remaining components and addition amounts are consistent with those of Example 1, and the preparation method is referred to Example 1.

[0073] Example 5

[0074] This embodiment provides an anti-acne composition with targeted antibacterial properties. The only difference between the anti-acne composition with targeted antibacterial properties and that of Example 1 is that the plant extract does not include myrtle extract, and the reduced amount thereof is distributed to the white willow bark extract and the pansy extract in proportion to the number of parts. The remaining components and addition amounts are consistent with those of Example 1, and the preparation method is referred to Example 1.

[0075] Example 6

[0076] This embodiment provides an anti-acne composition with targeted antibacterial properties. The only difference between the anti-acne composition with targeted antibacterial properties and that of Example 1 is that the plant extract does not include the pansy extract, and the reduced amount thereof is distributed to the white willow bark extract and the myrtle extract in proportion to the number of parts. The remaining components and addition amounts are consistent with those of Example 1, and the preparation method is referred to Example 1.

[0077] Comparative Example 1

[0078] This comparative example provides an anti-acne composition with targeted antibacterial properties. The anti-acne composition with targeted antibacterial properties is different from Example 1 only in that it does not include biological super protein, and its reduced amount is distributed to the plant extract and lactoglobulin in proportion. The preparation method refers to Example 1.

[0079] Comparative Example 2

[0080] This comparative example provides an anti-acne composition with targeted antibacterial properties. The anti-acne composition with targeted antibacterial properties is different from Example 1 only in that it does not include plant extracts, and the reduced amount thereof is distributed to the biosuper protein and lactoglobulin in proportion to the number of parts. The preparation method refers to Example 1.

[0081] Comparative Example 3

[0082] This comparative example provides an anti-acne composition with targeted antibacterial properties. The anti-acne composition with targeted antibacterial properties is different from Example 1 only in that it does not include lactoglobulin, and its reduced amount is distributed to the biological super protein and the plant extract in proportion. The preparation method refers to Example 1.

[0083] Application Example 1

[0084] This application example provides an acne-removing essence, the formula of the acne-removing essence is as follows by mass percentage: 10% of the targeted antibacterial acne-removing composition of Example 1, 2% of polysorbate-60, 3% of glyceryl stearate, 15% of moisturizer, 1% of xanthan gum, 0.8% of pH adjuster (sodium citrate), 2% of essence, and the balance is deionized water;

[0085] The moisturizing agent is erythritol, panthenol, squalane and niacinamide in a mass ratio of 0.5:3:2:3;

[0086] The preparation method of the acne-removing essence is as follows:

[0087] (1) Mixing the moisturizer and deionized water, heating the mixture to 60° C., and then adding polysorbate 60, glyceryl stearate, and xanthan gum and mixing until homogeneous;

[0088] (2) After cooling to 35° C., the anti-acne composition with targeted antibacterial properties and essence are added, and after mixing, a pH adjuster is added to adjust the pH of the system to 6.5-8, and the anti-acne essence is obtained after standing for 2 hours.

[0089] Application Example 2-6

[0090] This application example provides five acne-removing essences. The formulas of the acne-removing essences differ from those of Application Example 1 only in that the targeted antibacterial acne-removing compositions provided in Example 1 are replaced with the targeted antibacterial acne-removing compositions of Examples 2-6, respectively. The remaining components and proportions are consistent with those in Application Example 1, and the preparation method refers to that in Application Example 1.

[0091] Application Example 7

[0092] This application example provides an anti-acne essence. The formula of the anti-acne essence differs from that of Application Example 1 only in that the moisturizer is erythritol, panthenol and squalane in a mass ratio of 0.5:3:2, and the amount of moisturizer added remains unchanged. The remaining components and proportions are consistent with those in Application Example 1, and the preparation method refers to Application Example 1.

[0093] Application Example 8

[0094] This application example provides an anti-acne essence. The formula of the anti-acne essence differs from that of Application Example 1 only in that the moisturizer is erythritol, panthenol and niacinamide in a mass ratio of 0.5:3:3, and the added amount of the moisturizer is kept unchanged. The other components and proportions are consistent with those in Application Example 1, and the preparation method refers to Application Example 1.

[0095] Application Example 9

[0096] This application example provides an anti-acne essence. The formula of the anti-acne essence differs from that of Application Example 1 only in that the moisturizer is erythritol, squalane and niacinamide in a mass ratio of 0.5:2:3, and the added amount of the moisturizer is kept unchanged. The other components and proportions are consistent with those in Application Example 1, and the preparation method refers to Application Example 1.

[0097] Application Example 10

[0098] This application example provides an anti-acne essence. The formula of the anti-acne essence differs from that of Application Example 1 only in that the moisturizer is panthenol, squalane and niacinamide in a mass ratio of 3:2:3, and the added amount of the moisturizer is kept unchanged. The other components and proportions are consistent with those in Application Example 1, and the preparation method refers to Application Example 1.

[0099] Comparative Application Examples 1-3

[0100] This comparative application example provides three acne-removing essences. The formula of the acne-removing essence differs from that of Application Example 1 only in that the targeted antibacterial acne-removing composition provided in Example 1 is replaced by the targeted antibacterial acne-removing composition of Comparative Examples 1-3, respectively. The remaining components and proportions are consistent with those in Application Example 1, and the preparation method refers to that in Application Example 1.

[0101] Comparative Application Example 4

[0102] This comparative application example provides an acne-removing essence. The formula of the acne-removing essence differs from that of Application Example 1 only in that it does not contain an acne-removing composition with targeted antibacterial properties. The reduced amount is due to insufficient deionized water. The remaining components and proportions are consistent with those of Application Example 1. The preparation method refers to Application Example 1.

[0103] Test Example 1: Detection of the minimum inhibitory concentration of Propionibacterium acnes:

[0104] The product of Application Example 1 was formulated into a 100 mg / mL sample, and ten test tubes were prepared (the first of which was an empty test tube, and the other nine were filled with 5 ml of distilled water). 5 mL of sample was added to the first test tube; then 5 mL of sample was drawn into the second test tube and mixed; then 5 mL of diluent was drawn into the third test tube, and so on, when it came to the tenth test tube, 5 mL was drawn out of the tenth test tube and discarded. 0.1 mL of Propionibacterium acnes bacterial solution (100,000 cfu / mL) was added to each of the ten test tubes, mixed, and placed in a 37°C incubator for 24 hours, and the clear test tubes were observed to determine the minimum inhibitory concentration MIC.

[0105] The operations of application examples 2-6 and comparative application examples 1-4 are the same as above, and the results are shown in Table 1:

[0106] Table 1

[0107]

[0108]

[0109] It can be seen from the data in Table 1 that the anti-acne essence of the present invention has a significant inhibitory effect on Propionibacterium acnes, wherein the biological super protein, the plant extract and the lactoglobulin have a synergistic effect in inhibiting Propionibacterium acnes, and when the white willow bark extract, the myrtle extract and the pansy extract in the plant extract are present at the same time, the anti-acne essence has a better inhibitory effect on Propionibacterium acnes.

[0110] Test Example 2

[0111] Detection of anti-inflammatory effects on inflammatory factors:

[0112] (1) Dilute the Raw264.7 cells in good culture state to a concentration of 1×10 5cfu / mL;

[0113] (2) Prepare a 12-well cell plate and add a concentration of 1×10 5 cfu / mL of Raw264.7 cells were transferred to a 12-well plate using a pipette, 1 mL was added to each well, and cultured at 37°C for 24 h;

[0114] (3) The experiment included 21 groups, including a blank group (no sample added), an LPS group (1 μg / mL LPS added), an experimental group (2 mg / mL sample solutions of Application Examples 1-10 and Comparative Application Examples 1-4 added containing 1 μg / mL LPS), and a positive control group (0.1 mg / mL dexamethasone added). The supernatant of the Raw264.7 cell culture fluid was discarded, and the corresponding solutions of each group were added and cultured at 37°C for 24 h;

[0115] (4) The supernatant was centrifuged and the concentrations of inflammatory factors TNF-α and IL-6 were detected according to the test steps on the ELISA kit. Each group was measured 5 times in parallel and the average value was taken.

[0116] The results are shown in Table 2:

[0117] Table 2

[0118] Group TNF-α(pg / mL) IL-6 (pg / mL) Blank Group 32.69 0.84 LPS group 2301.25 193.29 Application Example 1 424.71 25.11 Application Example 2 440.13 28.01 Application Example 3 449.57 34.10 Application Example 4 507.18 58.31 Application Example 5 492.46 68.43 Application Example 6 496.23 71.52 Application Example 7 429.81 30.31 Application Example 8 438.82 34.52 Application Example 9 441.94 28.17 Application Example 10 430.22 30.94 Comparative application example 1 594.46 147.13 Comparative Application Example 2 590.13 128.62 Comparative Application Example 3 542.62 106.16 Comparative Application Example 4 1713.36 162.27 Positive control group 139.62 3.28

[0119] It can be seen from the data in Table 2 that the anti-acne essence involved in the present invention has a significant inhibitory effect on inflammatory factors TNF-α and IL-6, among which biological super protein, plant extract and lactoglobulin have a synergistic effect in inhibiting inflammatory factors. When the white willow bark extract, myrtle extract and pansy extract in the plant extract are present at the same time, the anti-acne essence has a better inhibitory effect on inflammatory factors.

[0120] Test Example 3

[0121] Moisturizing effect measurement

[0122] 60 healthy volunteers were selected, including 30 males and 30 females, aged 20 to 40 years old, without severe systemic diseases, immunodeficiency or autoimmune diseases, no allergies to cosmetics, no acute facial inflammation, no use of hormone drugs or immunosuppressants, no participation in other clinical trials, and no application of external preparations. They were randomly divided into 6 groups, 10 people in each group, and the samples provided in the above application examples 1, 7-10 and comparative application example 4 were applied respectively. The samples were applied to the inner skin of the arms of the volunteers in their respective groups. The water content of the inner skin of the arms was detected by a skin moisture tester (Corneometer CM825) at 0 min, 30 min, and 60 min, and the water content of the skin before and after the use of the samples was evaluated. The specific test results are shown in Table 3 below.

[0123] Table 3

[0124]

[0125] The data in Table 3 show that the anti-acne essence prepared in Application Example 1 has a good moisturizing effect, and the skin moisture content can reach more than 68% at 0h, still more than 62% after 30min, and more than 58% after 60min. It can be seen from Application Examples 7-10 that erythritol, panthenol, squalane and niacinamide in the moisturizer cooperate with each other, have a synergistic effect, and have a significant moisturizing and synergistic effect.

[0126] Test Example 4: Irritation Test

[0127] 140 volunteers aged 25-45 with facial acne were selected and randomly divided into 14 groups, with 10 volunteers in each group. The 14 groups of volunteers used the products of Application Examples 1-10 and Comparative Application Examples 1-4 for acne removal and mildness evaluation.

[0128] Test method: The subjects clean their faces with alkaline soap-based cleansing products under the guidance of the staff, and wipe them clean with a dandruff-free absorbent dry paper towel after cleaning. Sit quietly in a room that meets the requirements for at least 30 minutes, and do not drink water or beverages during this period. The laboratory researchers count the initial facial acne of each group of subjects. The subjects use the product once every morning after facial cleansing. The subjects return to the laboratory on the 10th and 20th days of using the product. Under the same test conditions, the laboratory researchers count the facial acne of the subjects. On the 20th day, the volunteers evaluate the mildness of the product. The scoring standards and grades are as follows:

[0129]

[0130] The acne removal test results and mildness evaluation results are shown in Table 4 (all data are presented as average values):

[0131] Table 4

[0132]

[0133]

[0134] It can be seen from the data in Table 4 that the acne-removing essence of the present invention has no irritation to the skin, indicating that the product of the present invention is safer and milder; from the comparison of the data of Comparative Application Example 1 and Application Examples 1-3, it can be seen that the essence of the present invention has excellent acne-removing effect after adding the acne-removing composition with targeted antibacterial properties, and the acne reduction rate can reach more than 40% after 20 days of use; from Application Examples 4-6, it can be seen that when the plant extract is not the type selected by the present invention, the acne-removing effect of the essence will be reduced; from Comparative Application Examples 1-3, it can be seen that when the essence of the present invention lacks any one of the biological super protein, plant extract or lactoglobulin, the acne-removing effect of the essence will be greatly reduced.

[0135] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a targeted antibacterial acne-removing composition and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0136] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0137] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. An acne-removing composition capable of targeted antibacterial treatment, characterized in that: The targeted antibacterial acne-removing composition comprises, by weight, 0.1-10 parts of biological super protein, 0.5-20 parts of plant extract and 0.1-5 parts of lactoglobulin; The plant extracts include white willow bark extract, myrtle extract and pansy extract.

2. The acne-removing composition capable of targeted antibacterial treatment according to claim 1, characterized in that: The targeted antibacterial acne-removing composition comprises, by weight, 1-8 parts of biological super protein, 3-15 parts of plant extract and 0.1-3 parts of lactoglobulin; Preferably, the anti-acne composition capable of targeted antibacterial activity comprises, by weight, 1-5 parts of biological super protein, 5-12 parts of plant extract and 0.1-1 part of lactoglobulin.

3. The anti-acne composition capable of targeted antibacterial treatment according to claim 1 or 2, characterized in that: The biological super protein is a mixture of bacteriophage lytic enzyme and collagen.

4. The acne-removing composition capable of targeted antibacterial treatment according to any one of claims 1 to 3, characterized in that: The mass ratio of the willow bark extract, myrtle extract and pansy extract is (1-4):(0.5-2):(0.5-3).

5. Use of the anti-acne composition capable of targeted antibacterial treatment as claimed in any one of claims 1 to 4 in an anti-acne product.

6. The use according to claim 5, characterized in that The acne-removing product includes acne-removing essence, acne-removing cream or acne-removing facial cleanser; Preferably, the mass percentage of the targetable antibacterial acne-removing composition in the acne-removing product is 1-20%.

7. An anti-acne essence, characterized in that: The anti-acne essence comprises the anti-acne composition with targeted antibacterial properties as claimed in any one of claims 1 to 4, an emulsifier, a moisturizer, a thickener and deionized water.

8. The anti-acne essence according to claim 7, characterized in that: The anti-acne essence comprises, by mass percentage, 1-20% of an anti-acne composition capable of targeted antibacterial treatment, 1-10% of an emulsifier, 1-20% of a moisturizer, 0.1-5% of a thickener, and 10-90% of deionized water; Preferably, the emulsifier is any one of steareth-2, steareth-21, glyceryl stearate, polysorbate-60, cetearyl glucoside, C12-20 alkyl glucoside, PEG-6 caprylic / capric glycerides or capryl / caprylamidopropyl betaine, or a combination of at least two thereof; Preferably, the thickener comprises any one of xanthan gum, carbomer or hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer or a combination of at least two thereof.

9. The anti-acne essence according to claim 7 or 8, characterized in that: The moisturizer includes any one or a combination of at least two of erythritol, panthenol, squalane, niacinamide, glycerin or 1,3-butylene glycol; Preferably, the moisturizer is a combination of erythritol, panthenol, squalane and niacinamide; Preferably, the mass ratio of erythritol, panthenol, squalane and niacinamide is (0.1-1):(2-5):(1-3):(2-4); Preferably, the anti-acne essence further comprises any one of a pH regulator, a preservative or a fragrance, or a combination of at least two of them.

10. The method for preparing the anti-acne essence according to any one of claims 7 to 9, characterized in that: The preparation method comprises: (1) mixing a moisturizer and deionized water, heating the mixture, and then adding an emulsifier and a thickener and mixing until homogeneous; (2) adding the acne-removing composition capable of targeted antibacterial treatment after cooling, mixing and allowing to stand to obtain the acne-removing essence; Preferably, the temperature in step (1) is raised to 50-70°C; Preferably, the temperature in step (2) is lowered to 25-35°C; Preferably, the standing time in step (2) is 1-3 hours; Preferably, step (2) further comprises adding any one or a combination of at least two of a pH adjuster, a preservative or a fragrance.

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