Medicinal preparation for treating closed soft tissue contusion and preparation method thereof

Abstract

The invention provides a medicinal preparation for treating closed soft tissue contusion and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw materials: rhizoma paridis, calcined native copper powder (calcined), gentiana macrophylla, teasel root, herba lycopi, safflower, vinegar frankincense, vinegar myrrh, rheum officinale, ground beeltle, processed semen strychni, angelica sinensis, scalded peach kernel, salted eucommia ulmoides, sappan wood, rhizoma drynariae, amber, pseudo-ginseng and dragon's blood. According to the medicinal preparation for treating closed soft tissue contusion and the preparation method, the medicinal material source is stable, and the production process is stable and reliable.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a pharmaceutical preparation for treating closed soft tissue contusion and a preparation method thereof. Background Art

[0002] Soft tissue contusion is a condition in which pathological damage occurs to human skin, superficial and deep subcutaneous fascia, muscles, tendons, tendon sheaths, ligaments, joint capsules, synovial capsules, intervertebral discs, and even peripheral nerves and blood vessels due to various acute traumas, chronic strains, and self-diseases. Among soft tissue contusions, closed soft tissue contusion is the most common, with various clinical manifestations, including local pain, swelling, tenderness, palpable nodules, cord-like structures, and hardening. It is also accompanied by joint swelling and pain, limited limb movement or abnormal sensation, and even joint deformity in severe cases. With the accelerated pace of urban life, changes in work methods, and increased mental stress, the incidence of soft tissue contusion is on the rise. Data from multiple fields such as health statistics, epidemiology, clinical and pathological anatomy show that the number of soft tissue contusions in my country has increased significantly. In view of this, the application prospects and market potential of pharmaceutical preparations for the treatment of closed soft tissue contusions are broad. Summary of the invention

[0003] The purpose of the present invention is to provide a pharmaceutical preparation and a preparation method for treating closed soft tissue contusion, with a stable source of medicinal materials and a stable and reliable production process.

[0004] The technical solution of the present invention is achieved in this way:

[0005] The invention provides a medicine preparation for treating closed soft tissue contusion, which is prepared from the following raw materials: paridis, calcined natural copper powder, gentiana macrophylla, dipsacus root, zedoaria, safflower, vinegar frankincense, vinegar myrrh, rhubarb, earthworm, processed strychnos nux vomica, angelica sinensis, stewed peach kernel, salt eucommia, sappan wood, drynaria, amber, notoginseng and dragon's blood.

[0006] As a further improvement of the present invention, the invention is prepared from the following raw materials in parts by weight: 4-8 parts of Paris polyphylla, 5-15 parts of calcined natural copper powder, 5-15 parts of Gentiana macrophylla, 5-15 parts of Dipsacus asper, 5-15 parts of Zedoariae rhizome, 5-15 parts of Carthamus tinctorius, 5-15 parts of vinegar frankincense, 5-15 parts of vinegar myrrh, 5-15 parts of rhubarb, 5-15 parts of Eucommia ulmoides, 5-15 parts of processed Strychnos nux vomica, 5-15 parts of Angelica sinensis, 5-15 parts of stewed peach kernel, 5-15 parts of salt Eucommia ulmoides, 5-15 parts of Sappan wood, 5-15 parts of Drynariae rhizome, 5-10 parts of amber, 5-15 parts of Panax notoginseng, and 5-10 parts of Dragon's Blood.

[0007] As a further improvement of the present invention, the invention is prepared from the following raw materials in parts by weight: 6 parts of Paris polyphylla, 10 parts of calcined natural copper powder, 10 parts of Gentiana macrophylla, 10 parts of Dipsacus asper, 10 parts of Zedoariae rhizome, 10 parts of Carthamus tinctorius, 10 parts of vinegar frankincense, 10 parts of vinegar myrrh, 10 parts of Rhubarb, 10 parts of Eucommia ulmoides, 10 parts of processed Strychnos nux vomica, 10 parts of Angelica sinensis, 10 parts of stewed peach kernel, 10 parts of salt Eucommia ulmoides, 10 parts of Sappan wood, 10 parts of Drynariae rhizome, 7 parts of amber, 10 parts of Panax notoginseng, and 7 parts of Dragon's Blood.

[0008] The present invention further protects a method for preparing the above-mentioned pharmaceutical preparation for treating closed soft tissue contusion, comprising the following steps:

[0009] Dragon's blood and calcined natural copper are crushed into fine powder, and other medicinal materials are crushed into coarse powder, mixed, soaked in white wine, percolated, the percolated liquid is collected, concentrated under reduced pressure, and white wine is added to adjust the ethanol content to obtain a pharmaceutical preparation for treating closed soft tissue contusion.

[0010] As a further improvement of the present invention, the particle size of the fine powder is 50-100 mesh.

[0011] As a further improvement of the present invention, the particle size of the coarse powder is 20-40 mesh.

[0012] As a further improvement of the present invention, the ethanol content in the liquor is 45-55wt%, and the soaking time is 45-55h.

[0013] As a further improvement of the present invention, the percolation rate is 100 mL / min / 18.29 kg crude drug.

[0014] As a further improvement of the present invention, the ethanol content in the pharmaceutical preparation for treating closed soft tissue contusion is 45-55wt%.

[0015] The present invention further protects the use of the above-mentioned pharmaceutical preparation for treating closed soft tissue contusion in the preparation of drugs for treating and assisting in treating closed soft tissue contusion.

[0016] The invention has the following beneficial effects: the calcined natural copper powder in the prescription has the effects of dispersing blood stasis, relieving pain, and setting bones and tendons, and is the main drug. Safflower can promote blood circulation and relieve menstruation, remove blood stasis and relieve pain; rhubarb can break up stagnation and promote blood stasis, and is the assistant drug. Frankincense and myrrh can promote blood circulation and relieve pain, reduce swelling and promote tissue regeneration; Dipsacus can nourish the kidney, promote blood circulation, and fix tendons and bones, and are the adjuvant drugs. Eupolyphaga sinensis can break up blood circulation and remove blood stasis, relieve menstruation and relieve pain, fix tendons and fix bones, and lead the medicine directly to the diseased area, and is the adjuvant drug. All the drugs are used together to achieve the effects of relaxing tendons and activating collaterals, reducing swelling and dispersing blood stasis, and setting bones and relieving pain. For external use, apply an appropriate amount to the affected area.

[0017] After more than 50 years of clinical application, this drug preparation has been proven to be effective in treating bone-related diseases, relieving pain from falls, reducing swelling and dispersing blood stasis. It can be used for all trauma, falls, bruises, redness and swelling, and has significant efficacy. Clinical studies have found that the external use of drug preparations combined with manipulation to treat acute lumbar sprains is significantly different from that of simple manipulation, and the total effective rate of the treatment group reached 97.28%. The use of medicinal wine not only avoids the trouble of decocting Chinese medicine decoctions, but is also convenient to use, and is deeply welcomed by patients, and is worthy of widespread clinical promotion and application. All of the above indicate that the development of drug preparations to treat closed soft tissue contusions has a very broad application prospect and market potential, providing a new option for the treatment of closed soft tissue contusions, and the preparation method of drug preparations for treating closed soft tissue contusions provides patients with closed soft tissue contusions with more reliable and effective pure Chinese medicine preparations.

[0018] The pharmaceutical preparation for treating closed soft tissue contusion and the preparation method of the present invention have stable sources of medicinal materials and stable and reliable production process. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0020] Figure 1 Comparison of swelling of the right hind limbs of rats in each group after modeling;

[0021] Figure 2 Comparison of swelling of the right hind limbs of rats in each group after drug administration;

[0022] Figure 3 Comparison of swelling degree of hind limbs of rats with soft tissue injury;

[0023] Figure 4 Comparison of the levels of inflammatory factors IL-1β and IL-6 in rat soft tissue homogenate;

[0024] Figure 5 Comparison of inflammatory factors TNF-α and PGE2 levels in rat soft tissue homogenate;

[0025] Figure 6 Comparison of MDA and SOD levels in rat soft tissue homogenate;

[0026] Figure 7 Comparison of HE-stained sections of rat soft tissue (×200);

[0027] Figure 8 Comparison of drug preparations on the writhing reaction of mice induced by glacial acetic acid;

[0028] Fig. 9 Comparison of the analgesic effects of drug preparations on hot plate method-induced pain in mice. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Example 1

[0031] Raw material composition (by weight): 4 parts of Paris polyphylla, 5 parts of calcined natural copper powder, 5 parts of Gentiana macrophylla, 5 parts of Dipsacus asper, 5 parts of Zedoariae rhizome, 5 parts of Carthamus tinctorius, 5 parts of vinegar frankincense, 5 parts of vinegar myrrh, 5 parts of Rhubarb, 5 parts of Eucommia ulmoides, 5 parts of processed Strychnos nux vomica, 5 parts of Angelica sinensis, 5 parts of stewed peach kernel, 5 parts of salt Eucommia ulmoides, 5 parts of Sappan wood, 5 parts of Drynariae rhizoma, 5 parts of amber, 5 parts of Panax notoginseng, and 5 parts of Dragon's Blood.

[0032] The preparation method comprises the following steps:

[0033] Dragon's blood and calcined natural copper are crushed into fine powder (50 mesh), and the other medicinal materials are crushed into coarse powder (20 mesh), mixed, moistened with 22L of 50% liquor for 1 hour and then loaded into a cylinder, soaked with 40L of 45wt% liquor for 45 hours, and slowly percolated with 200L of 45wt% liquor at a speed of 100mL / min / 18.29kg of crude drug, the percolated liquid is collected, concentrated under reduced pressure, the alcohol content is adjusted to 45wt%, and mixed to obtain a pharmaceutical preparation for treating closed soft tissue contusion.

[0034] Example 2

[0035] Raw material composition (by weight): 8 parts of Paris polyphylla, 15 parts of calcined natural copper powder, 15 parts of Gentiana macrophylla, 15 parts of Dipsacus asper, 15 parts of Zedoariae rhizome, 15 parts of Carthamus tinctorius, 15 parts of vinegar frankincense, 15 parts of vinegar myrrh, 15 parts of Rhubarb, 15 parts of Eucommia ulmoides, 15 parts of processed Strychnos nux vomica, 15 parts of Angelica sinensis, 15 parts of stewed peach kernel, 15 parts of salt Eucommia ulmoides, 15 parts of Sappan wood, 15 parts of Drynariae rhizome, 10 parts of amber, 15 parts of Panax notoginseng, and 10 parts of Dragon's Blood.

[0036] The preparation method comprises the following steps:

[0037] Dragon's blood and calcined natural copper are crushed into fine powder (100 mesh), and the other medicinal materials are crushed into coarse powder (40 mesh), mixed, moistened with 22L of 50% liquor for 1 hour and then loaded into a cylinder, soaked with 40L of 55wt% liquor for 55 hours, and slowly percolated with 200L of 55wt% liquor at a speed of 100mL / min / 18.29kg of crude drug, the percolated liquid is collected, concentrated under reduced pressure, the alcohol content is adjusted to 55wt%, and mixed to obtain a pharmaceutical preparation for treating closed soft tissue contusion.

[0038] Example 3

[0039] Raw material composition (by weight): 6 parts of Paris polyphylla, 10 parts of calcined natural copper powder, 10 parts of Gentiana macrophylla, 10 parts of Dipsacus asper, 10 parts of Zedoariae rhizome, 10 parts of Carthamus tinctorius, 10 parts of vinegar frankincense, 10 parts of vinegar myrrh, 10 parts of Rhubarb, 10 parts of Eucommia ulmoides, 10 parts of processed Strychnos nux vomica, 10 parts of Angelica sinensis, 10 parts of stewed peach kernel, 10 parts of salt Eucommia ulmoides, 10 parts of Sappan wood, 10 parts of Drynariae rhizome, 7 parts of amber, 10 parts of Panax notoginseng, and 7 parts of Dragon's Blood.

[0040] The preparation method comprises the following steps:

[0041] Dragon's blood and calcined natural copper are crushed into fine powder (60 mesh), and the other medicinal materials are crushed into coarse powder (24 mesh), mixed, moistened with 22L of 50% liquor for 1 hour and then loaded into a cylinder, soaked with 40L of 50wt% liquor for 50 hours, and slowly percolated with 200L of 50wt% liquor at a speed of 100mL / min / 18.29kg of crude drug, the percolated liquid is collected, concentrated under reduced pressure, the alcohol content is adjusted to 50wt%, and mixed to obtain a pharmaceutical preparation for treating closed soft tissue contusion.

[0042] Test Example 1 Treatment of acute soft tissue injury rat model

[0043] 1.1 Model building method

[0044] Take 50 SD male rats, shave the hair of the right hind limb thigh of the rat first, and then use 6% sodium sulfide solution to further remove the residual hair. Before modeling, use a vernier caliper to measure the diameter of the thigh muscle of the left and right hind limbs of each rat, and then randomly select 8 rats as the blank group without modeling, and the remaining rats are anesthetized by intraperitoneal injection of 20% urethane solution. A 30cm long PVC plastic tube is fixed vertically on the iron frame in advance, and the right hind limb of the anesthetized rat is bent and fixed flat on the iron frame, and is directly below the plastic tube. The 200g weight is free-falling from the top of the plastic tube so that it hits the right hind limb thigh muscle of the rat, and is repeated 5 times to cause acute soft tissue injury in the rat. The successful modeling is manifested by obvious hemorrhages, bruises and swelling under the thigh skin by naked eye observation and hand touch, but there is no bleeding and damage to the skin, no open injuries such as joint dislocation and fracture, and other parts of the rat are normal. Otherwise, the modeling was unsuccessful. The rats with unsuccessful modeling were eliminated and the rats with successful modeling were selected as the experimental observation objects. In this modeling, two rats were eliminated due to fractures, and the remaining 40 rats were successfully modeled.

[0045] 1.2 Grouping scheme and drug intervention

[0046] The 40 rats with successful modeling were randomly divided into model group, positive group, high dose, medium dose and low dose groups of drug preparations, with 8 in each group. The dosage for each administration was: blank group (administered 5mL / kg normal saline), model group (administered 5mL / kg matrix liquor), positive group (Voltaren 500mg / kg), high dose group (915mg / kg), medium dose group (457mg / kg) and low dose group (229mg / kg). Before administration, the diameter of the thigh muscle of the left and right hind limbs of each rat was measured with a vernier caliper to calculate the swelling. Each group of rats was smeared with the corresponding medicine at the soft tissue injury site, the blank group was smeared with normal saline, and the model group was smeared with liquor matrix, and the administration volume was 5mL / kg. The drug preparation group used a 1mL syringe to accurately draw the drug volume obtained in Example 3 and evenly smeared it on the rat thigh injury, and then smeared with a cotton swab for 1-2min to promote drug absorption, and was administered twice a day for 3 consecutive days.

[0047] 1.3 Index detection

[0048] After the last administration, the diameter of the thigh muscle of the left and right hind limbs of each rat was measured with a vernier caliper on the morning of the second day to calculate the swelling degree. The rats were anesthetized, the skin of the injured part of the right hind thigh of the rat was cut open, the subcutaneous tissue was separated, and part of the muscle tissue was cut. After rinsing the blood on the muscle tissue with PBS solution, the water was absorbed by filter paper, and 0.2g was cut. A 10% homogenate was prepared in an ice water bath with a glass homogenizer. The prepared homogenate was centrifuged at 3000r / min for 10 minutes, and the supernatant was taken. The levels of IL-1β, IL-6, TNF-α, and PGE2 factors were determined by ELISA, the content of MDA was detected by TBA method, and the activity of SOD was detected by WST-1 method; another part of the muscle tissue was fixed with 4% paraformaldehyde solution, and HE staining sections were made for soft tissue pathological morphology examination. Swelling degree (cm) = right hind limb muscle diameter - left hind limb muscle diameter.

[0049] 1.4 Research Results

[0050] A. Swelling of the right hind limbs of rats in each group after modeling and drug administration

[0051] Except for the blank group, the rats in the other groups had obvious bruises and swelling under the skin of the right hind thigh after modeling, but there was no skin damage and bleeding, and no open fractures, indicating that the soft tissue injury model was successfully established. After drug treatment, the swelling of the right hind limb of the rats in the model group was alleviated to a certain extent, but not significantly; the bruises and swelling of the right hind limb of the rats in the positive group (Voltaren) and the high, medium and low dose groups of the drug preparation were significantly alleviated, as shown in Figure 2. Figure 1 , 2 .

[0052] B. Effect on swelling of hind limbs of rats with soft tissue injury

[0053] The average swelling degree of the hind limbs of rats in each group increased significantly after modeling, and there were significant differences compared with the blank group (P<0.01). After administration, the drug group could significantly reduce the swelling degree of the hind limbs of rats, and there was a significant difference between the high-dose group and the model group (P<0.05). Figure 3 .

[0054] C. Effects on the levels of inflammatory factors IL-1β, IL-6, TNF-α, and PGE2

[0055] Compared with the blank group, the levels of IL-1β, IL-6, TNF-α, and PGE2 in the tissue homogenate of the model group rats were significantly increased (P<0.01). Compared with the model group, the drug preparation group could significantly reduce the levels of IL-1β, IL6, TNF-α, and PGE2 (P<0.01 or P<0.05). Figure 4 , 5 .

[0056] D. Effects on oxidation indexes of soft tissue homogenate

[0057] Compared with the blank group, the MDA level in the model group was significantly increased, and the SOD activity was significantly decreased (P<0.05). Compared with the model group, the high and medium doses of the drug preparation could significantly reduce the MDA level (P<0.05); the high, medium and low doses of the drug preparation could significantly increase the SOD activity (P<0.05). Figure 6 .

[0058] E. Effects on pathological changes of soft tissue and muscles

[0059] The muscle bundles in the muscle tissue of the rats in the blank group were of different sizes, the muscle cells were thin and long, fibrous or irregular, arranged tightly and neatly, the sarcoplasm was evenly stained, the nuclei were flat or oval, with little chromatin and obvious nucleoli, and no obvious degeneration or necrosis was observed. Blood vessels, nerves, etc. were visible in the muscles, without obvious inflammatory cell infiltration and fibrous tissue hyperplasia, and no obvious pathological morphological changes were observed. Compared with the blank group, the muscle bundles in the model group were disordered, the muscle fibers were dissolved and necrotic, and there was infiltration of inflammatory factors, and the tissue damage was obvious; compared with the model group, the muscle bundles in the drug preparation group were slightly disordered, the necrosis was significantly reduced, the inflammatory factors were reduced, and the degree of damage was significantly reduced. Figure 7 .

[0060] Test Example 2 Analgesic effect of writhing-induced pain in mice

[0061] 2.1 Methods

[0062] Take 4-week-old mice, half male and half female, with a body weight of (20±2)g, and randomly divide them into model group, positive group, high, medium and low concentration groups of drug preparation, a total of 5 groups, 10 in each group. The abdomen of each group of mice was smeared with the corresponding drug for preventive administration. The high, medium and low concentration groups of drug preparation were respectively given the drug preparations prepared in Example 3 with drug concentrations of 182.9mg / mL, 150mg / mL and 116mg / mL. The positive group was given Yunnan Baiyao tincture, and the model group was given liquor. The administration volume was 750μL / time, and the administration was given twice a day for 6 consecutive days. On the 7th day, the drug was applied once again. After the last administration, 0.6% glacial acetic acid solution 0.1mL / 10g body weight was injected intraperitoneally 1h later. The number of twists of mice within 15min after injection was recorded, and the twist inhibition rate was calculated. Twist inhibition rate (%) = (mean number of twists in the model group-mean number of twists in the drug administration group) / mean number of twists in the model group × 100%.

[0063] 2.2 Research Results

[0064] Compared with the model group, the writhing times of the high, medium, and low concentration groups of the drug preparation and the positive group were significantly reduced (P<0.01), and the writhing inhibition rates of the high, medium, and low concentration groups were 68.89%, 50.37%, and 33.83%, respectively (see Figure 8 ).

[0065] Test Example 3 Analgesic effect of hot plate method-induced pain in mice

[0066] 3.1 Methods

[0067] SPF grade Kunming female mice, 4 weeks old, with a body weight of (20±2)g, were selected. The hot plate analgesia was set at (55±0.5)℃, and the pre-drug pain threshold (i.e., the time required for the mouse to lick its hind feet from the time it was placed in the body) was screened. Qualified mice with a pre-drug pain threshold of 5 to 30s were screened and evenly divided into a model group, a positive group, and a high, medium, and low concentration group of the drug preparation according to the pain threshold, a total of 5 groups, 10 in each group. The left and right feet of each mouse were smeared with the corresponding drugs for preventive administration: the high, medium, and low concentration groups of the drug preparation were given medicinal liquor with a crude drug concentration of 182.9mg / mL, 150mg / mL, and 116mg / mL, respectively, the positive group was given Yunnan Baiyao tincture, and the model group was given matrix liquor. The administration volume of each group was 550μL of the drug solution for each left and right foot, once a day, for 3 consecutive days. Continue to apply the drug once on the 4th day. The pain threshold of each mouse was measured at 30, 60, and 90 minutes after the last administration. The pain threshold of each group was counted and the pain threshold increase rate (%) was calculated. Pain threshold increase rate (%) = (mean pain threshold after administration - mean pain threshold before administration) / mean pain threshold before administration × 100%.

[0068] 3.2 Research Results

[0069] There was no significant difference in pain thresholds before administration among the groups (P>0.05). Compared with the model group, the pain thresholds of the high-concentration group and the positive group were significantly increased at 30 minutes, 60 minutes, and 90 minutes after the last administration (P<0.05 or P<0.01), and the pain threshold of the medium-concentration group was significantly increased at 30 minutes after administration (P<0.01). There was no significant difference in pain thresholds at each time point between the low-concentration group and the model group (P>0.05). Fig. 9 ).

[0070] Test Case 4 Clinical Study

[0071] In a clinical study, 180 outpatients with acute soft tissue injuries were treated with drug preparations. The results showed that after 4 and 7 days of treatment, the drug preparation had a significant effect on acute soft tissue injuries compared with before treatment (P>0.05), and could effectively improve the swelling symptoms of patients. At the same time, the visual analogue scale (VAS) and TCM symptom scores of patients gradually decreased with the extension of medication time.

[0072] In a 4-year clinical study, 45 patients with soft tissue injuries were treated with the drug preparation. The results showed that the total effective rate of the drug preparation in treating soft tissue injuries reached 91.11%. This data shows that the drug preparation has a high clinical efficacy in treating soft tissue injuries and can provide an effective treatment plan for patients.

[0073] Zhang, male, 28 years old, from Guigang, Guangxi. Due to careless exercise, the right ankle was sprained, with local swelling, pain, and limited activity. After conventional detumescence and analgesia treatment in the hospital, the swelling was slightly relieved, but the pain was still obvious and walking was difficult. After discharge, the pharmaceutical preparation prepared in Example 3 was used, twice a day, applied to the affected area each time. After continuous use for 1 week, the swelling subsided, the pain was relieved, and walking returned to normal.

[0074] Wang, female, 45 years old, from Nanning, Guangxi. She was accidentally hit by a heavy object on her left forearm at work, which caused soft tissue injury, local bruise and swelling, unbearable pain, and limited movement. After cold compress, swelling and pain relief in the hospital, the swelling was slightly relieved, but the bruises did not disappear, the pain was still obvious, and affected her daily work. After leaving the hospital, she continued to use the drug preparation, twice a day, applying it to the affected area each time. After 10 days of use, the bruises disappeared significantly, the pain was relieved, and the arm movement returned to normal.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A pharmaceutical preparation for treating closed soft tissue contusion, characterized in that: The invention is prepared from the following raw materials: Paris polyphylla, calcined natural copper powder, Gentiana macrophylla, Dipsacus asper, Eupatorium fortunei, Safflower, vinegar frankincense, vinegar myrrh, Rhubarb, Eupolyphaga sinensis, processed Strychnos nux vomica, Angelica sinensis, stewed peach kernel, salted Eucommia ulmoides, Sappan wood, Drynaria fortunei, amber, Panax notoginseng, and Dragon's blood.

2. The pharmaceutical preparation for treating closed soft tissue contusion according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 4-8 parts of Paris polyphylla, 5-15 parts of calcined natural copper powder, 5-15 parts of Gentiana macrophylla, 5-15 parts of Dipsacus asper, 5-15 parts of Zedoariae rhizome, 5-15 parts of Carthamus tinctorius, 5-15 parts of vinegar frankincense, 5-15 parts of vinegar myrrh, 5-15 parts of rhubarb, 5-15 parts of Eucommia ulmoides, 5-15 parts of processed Strychnos nux vomica, 5-15 parts of Angelica sinensis, 5-15 parts of stewed peach kernel, 5-15 parts of salted Eucommia ulmoides, 5-15 parts of Sappan wood, 5-15 parts of Drynariae rhizome, 5-10 parts of amber, 5-15 parts of Panax notoginseng and 5-10 parts of Dragon's Blood.

3. The pharmaceutical preparation for treating closed soft tissue contusion according to claim 2, characterized in that: The invention is prepared from the following raw materials in parts by weight: 6 parts of Paris polyphylla, 10 parts of calcined natural copper powder, 10 parts of Gentiana macrophylla, 10 parts of Dipsacus asper, 10 parts of Zedoariae rhizome, 10 parts of Carthamus tinctorius, 10 parts of vinegar frankincense, 10 parts of vinegar myrrh, 10 parts of Rhubarb, 10 parts of Eucommia ulmoides, 10 parts of processed Strychnos nux vomica, 10 parts of Angelica sinensis, 10 parts of stewed peach kernel, 10 parts of salt Eucommia ulmoides, 10 parts of Sappan wood, 10 parts of Drynariae rhizome, 7 parts of amber, 10 parts of Panax notoginseng and 7 parts of Dragon's Blood.

4. A method for preparing a pharmaceutical preparation for treating closed soft tissue contusion according to any one of claims 1 to 3, characterized in that: The following steps are involved: Dragon's blood and calcined natural copper are crushed into fine powder, and other medicinal materials are crushed into coarse powder, mixed, soaked in white wine, percolated, the percolated liquid is collected, concentrated under reduced pressure, and white wine is added to adjust the ethanol content to obtain a pharmaceutical preparation for treating closed soft tissue contusion.

5. The preparation method according to claim 4, characterized in that: The particle size of the fine powder is 50-100 meshes.

6. The preparation method according to claim 4, characterized in that: The particle size of the coarse powder is 20-40 meshes.

7. The preparation method according to claim 4, characterized in that: The ethanol content in the liquor is 45-55wt%, and the soaking time is 45-55h.

8. The preparation method according to claim 4, characterized in that: The percolation rate is 100 mL / min / 18.29 kg crude drug.

9. The preparation method according to claim 4, characterized in that: The ethanol content in the pharmaceutical preparation for treating closed soft tissue contusion is 45-55wt%.

10. Use of the pharmaceutical preparation for treating closed soft tissue contusion according to any one of claims 1 to 3 in preparing drugs for treating and assisting in treating closed soft tissue contusion.

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