Biological agent for bottom mud-free lake treatment, preparation method and water quality self-purification device

By using biological fungi agents containing a variety of microorganisms in bottomless mud lakes, combined with mobile devices, liquid surface floating island parts and double-layer suction parts, the problems of difficulty in treating bottomless mud lakes and stockpiling of bottoms are solved, and the self-purification and ecological restoration of the lake water are achieved.

CN119874051AActive Publication Date: 2025-04-25QINGDAO HONGYANG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510160713.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-13
Publication Date
2025-04-25
Estimated Expiration
2045-02-13

AI Technical Summary

Technical Problem

The bottom of the lake without bottom mud lake does not have the ability to heal, which makes it difficult to control. The traditional biological fungal agent dispensing device is not convenient for self-mobile cleaning, the cleaning area is small, it is not convenient to set cleaning routes according to the shape of the lake, and it is not convenient to collaborate with the natural precipitation of bacteria agents, which is easy to cause the accumulation of bottom mud.

Method used

It provides a biological bacteria agent for bottomless mud lake treatment, including Bacillus subtilis, photosynthetic bacteria, nitrifying bacteria, denitrifying bacteria, thiobacteria, anaerobic ammonia oxidizing bacteria, phosphorus lysis bacteria, sulfate reducing bacteria, lactic acid bacteria and yeast. It is added and treated through a self-purifying device for water quality, and uses components such as mobile devices, liquid surface floating islands and double-layer suction parts to realize self-purification and ecological restoration of lake water.

Benefits of technology

Through the metabolic effects of biological bacteria agents, remove pollutants such as organic matter in water, degrade COD, BOD, ammonia nitrogen, total phosphorus and other indicators, inhibit the growth of cyanobacteria, and realize self-purification and ecological restoration of the water quality of bottomless artificial lakes, effectively solve the problems of cyanobacteria outbreaks and black odor, and improve the comprehensiveness and purification effect of the lake.

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Abstract

The invention discloses a biological agent for bottom mud-free lake treatment, a preparation method and a water quality self-purification device. The biological agent is prepared from the following formula: 5 to 95 parts of bacillus subtilis, 5 to 95 parts of photosynthetic bacteria, 10 to 80 parts of nitrifying bacteria, 10 to 80 parts of denitrifying bacteria, 5 to 75 parts of thiobacillus, 5 to 75 parts of anaerobic ammonium oxidation bacteria, 3 to 70 parts of phosphate solubilizing bacteria, 3 to 70 parts of sulfate reducing bacteria, 10 to 70 parts of lactic acid bacteria and 10 to 60 parts of saccharomycetes. The microbial agent adding part contains a biological microbial agent for treating the bottom mud-free lake; through the water quality self-purification device and the addition of the biological agent, pollutants such as organic matters in a water body are removed by utilizing the metabolism of microorganisms, indexes such as COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), ammonia nitrogen and total phosphorus are degraded, and the growth of blue-green algae is inhibited, so that the self-purification and ecological restoration of the water quality of the bottom-mud-free artificial lake are realized, and the problems of blue-green algae outbreak and black and odorous blue-green algae are effectively solved.
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Description

Technical Field

[0001] The invention relates to the technical field of lake water treatment, and in particular to a biological bacterial agent for treating a bottomless lake, a preparation method and a water quality self-purification device. Background Art

[0002] "No bottom mud" refers to an artificial lake with a cement bottom. Because of the cement bottom, the bottom of the lake does not have the ability to self-heal, and it is difficult to manage. Therefore, our biological island and bacterial agents are needed for management. However, since the existing bacterial agents are directly added to specific locations of the lake manually, the operation is relatively unchanged. Even if traditional delivery devices are used for delivery, the traditional delivery devices are not convenient for self-propelled mobile cleaning, the cleaning area is small, and it is not convenient to set the cleaning route according to the shape of the lake. It is also not convenient to quickly recover the precipitated impurities after the natural precipitation of the bacterial agent, which easily causes bottom mud accumulation.

[0003] To this end, we propose a biological bacterial agent for the treatment of bottomless lakes, a preparation method and a water quality self-purification device. Summary of the invention

[0004] The purpose of the present invention is to provide a biological bacterial agent for the treatment of a bottomless lake, a preparation method and a water quality self-purification device, so as to solve the problem mentioned in the above background technology that the bottom of the current "bottomless lake" has no self-healing ability and is difficult to treat.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a biological agent for the treatment of a bottomless mud lake, characterized in that the biological agent comprises the following raw materials in parts by weight: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria and 10-60 parts of yeast.

[0006] Preferably, the biological agent also includes: nutrients, trace elements, stabilizers and protective agents.

[0007] Preferably, the nutrients include at least one of a carbon source, a nitrogen source, a phosphorus source, an inorganic salt and a trace element.

[0008] Preferably, the stabilizer comprises at least one of glycerin, gelatin and lactitol.

[0009] Preferably, the protective agent includes at least one of sodium alginate, glycerol and β-cyclodextrin.

[0010] A method for preparing a biological agent for treating a bottomless lake comprises the following steps:

[0011] S1. Activation culture: inoculate the preserved bacteria into fresh culture medium for composite activation culture;

[0012] S2. Expanded culture: The activated strains are inoculated into a larger culture container for expanded culture. The culture conditions are controlled to promote the rapid growth and reproduction of the strains. During the culture process, samples are taken regularly to test the concentration and activity of the strains to ensure that the growth of the strains meets the requirements.

[0013] S3, homogenization treatment: by stirring or oscillating, the mixed bacteria and nutrients, trace elements, stabilizers and protective agents are fully mixed to form a homogenized bacterial agent;

[0014] S4. Packaging: The prepared bacterial agent is packaged. The packaging container should be made of sterile and well-sealed materials, such as glass bottles and plastic bottles;

[0015] S5. Storage: Store the packaged microbial agent in a cool, dry, dark place. At the same time, check the storage condition of the microbial agent regularly.

[0016] A water quality self-purification device for treating a bottomless mud lake comprises a purification installation part, on which a moving device is installed, characterized in that: the moving device is used to move and adjust the purification position; a row of fish-driving control parts are installed on the purification installation part; the fish-driving control parts are used to drive away fish schools;

[0017] The mobile device is provided with a bacterial agent adding unit;

[0018] The bacterial agent adding part contains biological bacterial agents for treating bottom-sludge lakes;

[0019] A liquid floating island is installed on the bacterial agent adding part; the liquid floating island is used to aerate the lake water;

[0020] The mobile device is provided with a brushing auxiliary part, and the brushing auxiliary part is used to clean the floating island part on the liquid surface;

[0021] A double-layer suction piece is installed on the mobile device; the double-layer suction piece is used to prevent damage to aquatic plants;

[0022] The purification mounting component includes: a guide mounting seat and a raised guide rail, and a row of through holes are respectively provided on both sides of the guide mounting seat; the through holes on both sides of the guide mounting seat are used to pass bolts to install the guide mounting seat on the bottom of the water; the raised guide rail is fixedly installed on the guide mounting seat, and the guide mounting seat is composed of two round steel columns; the raised guide rail is used to guide the movement of the moving device.

[0023] Preferably, the purification mounting part further comprises: a fish-repelling seat and an underwater buzzer speaker; a row of fish-repelling seats are fixedly mounted on the side of the guide mounting seat by bolts, and underwater buzzers are respectively fixedly mounted on a row of guide mounting seats; the underwater buzzers are used to drive away fish.

[0024] Preferably, the mobile device comprises: a mobile bracket, a mobile extrusion rod, a waterproof driving motor, a driving groove wheel, a driven wheel frame, a spring shaft and a fitting groove wheel, wherein the top of the mobile bracket is a circular structure; the mobile bracket is located outside the raised guide rail; a mobile extrusion rod is fixedly installed at the bottom of the mobile bracket, and the bottom of the mobile extrusion rod is an arc structure; two waterproof driving motors are fixedly installed on the mobile bracket; driving groove wheels are fixedly installed on the output shafts of the two waterproof driving motors, and the ends of the two driving groove wheels are rotatably installed on the mobile bracket respectively; the two driving groove wheels are attached to the raised guide rail; a circle of meshing teeth are respectively provided on the top of the two driving groove wheels; two driven wheel frames are attached to the inner side of the mobile bracket; two spring shafts are fixedly installed on the two driven wheel frames, and the four spring shafts are respectively slidably inserted on the mobile bracket; springs are respectively sleeved on the four spring shafts; two fitting groove wheels are respectively rotatably installed on the two driven wheel frames, and the two fitting groove wheels are elastically attached to the raised guide rail respectively; the driving groove wheel and the fitting groove wheel are used for rolling on the raised guide rail.

[0025] Preferably, the fish-driving control component includes: a control mounting tube, a pressure head and a delayed piezoelectric switch, the control mounting tube is fixedly sleeved on the fish-driving seat; the control mounting tube is aligned with the movable extrusion rod; the pressure head is slidably inserted on the control mounting tube, and the top of the pressure head is a hemispherical structure; a spring is provided at the bottom of the pressure head, and the spring at the bottom of the pressure head is sleeved inside the control mounting tube; the movable extrusion rod is used for moving and extruding the pressure head; a delayed piezoelectric switch is fixedly installed inside the control mounting tube, and the delayed piezoelectric switch is located at the bottom of the pressure head; the delayed piezoelectric switch is electrically fused and connected to an underwater buzzer speaker.

[0026] Preferably, the bacterial agent adding part includes: a lifting installation column, a lifting spray ring and a spacing adaptation spring, the lifting installation column is fixedly installed on the movable bracket; the upper section of the lifting installation column is a hexagonal column structure; a row of lifting spray rings is slidably sleeved on the lifting installation column; a circle of nozzles are respectively arranged on the outer side of a row of the lifting spray rings; a row of lifting spray rings are connected by a hose; a spacing adaptation spring is arranged between a row of the lifting spray rings, and a row of spacing adaptation springs are respectively sleeved on the lifting installation column; a row of spacing adaptation springs have the same elastic force; a row of spacing adaptation springs is used to space a row of lifting spray rings.

[0027] Preferably, the liquid surface floating island component includes: a floating island box, an isolation mesh, a counterweight lead ring, a water filter net, a bacteria agent box and a suction pump, wherein a foam float is provided at the bottom of the floating island box; the floating island box is slidably plugged into the hexagonal column at the upper section of the lifting installation column; an isolation mesh is fixedly installed at the bottom of the floating island box; a row of counterweight lead rings is fixedly installed on the isolation mesh; the counterweight lead ring at the bottom is respectively connected to the side of the movable extrusion rod and the movable bracket through two steel wires; the floating island box is used to float on the liquid surface; the counterweight lead ring is used to pull down the isolation mesh; A water filter net is installed; the water filter net is used to isolate impurities, and the bacteria agent box is fixedly installed on the floating island box; an opening is provided on the top of the bacteria agent box, and the bacteria agent box is used to store the biological bacteria agent; a water pump is fixedly installed on the bottom of the bacteria agent box, and the water pump passes through the floating island box; the water outlet pipe of the water pump is connected to the upper lifting spray ring; an aeration pump is fixedly installed on the top of the floating island box, and the jet pipe of the aeration pump passes through the floating island box, and the jet pipe of the aeration pump is located inside the isolation mesh; the suction pump is fixedly installed on the floating island box; the suction pump is used to suck out precipitated impurities.

[0028] Preferably, the brushing auxiliary part includes: a brushing rotating ring and a cleaning brush, the brushing rotating ring is rotatably sleeved on the movable bracket; a gear is provided on the inner side of the brushing rotating ring, and the gear on the inner side of the brushing rotating ring is engaged with the meshing teeth on the top of the two driving groove wheels; a cleaning brush is fixedly installed on the side of the brushing rotating ring, and a row of elastic soft bristles is provided on the cleaning brush; the row of elastic soft bristles on the cleaning brush is used for brushing the isolation mesh.

[0029] Preferably, the double-layer suction main pipe includes: a double-layer suction main pipe, an inner pipe and an anti-adsorption sleeve, the double-layer suction main pipe is fixedly installed on the mobile bracket through a bracket; a row of inner pipes is fixedly installed on the double-layer suction main pipe, and absorption holes are provided on the outer side of the inner pipe; the inner pipe is a rubber hose; the double-layer suction main pipe is connected to the water inlet of the suction pump through a hose, and a row of anti-adsorption sleeves is fixedly installed on the double-layer suction main pipe, and a row of anti-adsorption sleeves are respectively located outside a row of inner pipes; the anti-adsorption sleeves are rubber hoses; the anti-adsorption sleeves are used to reduce damage to aquatic plants.

[0030] Principles of sewage treatment

[0031] Biological islands replace bottom mud to carry microorganisms: biological fillers for microorganisms to attach and grow, such as ecological expanded clay, activated carbon, etc., are selected to provide a living environment for probiotics.

[0032] Strong reproductive capacity: The microbial population in the microbial agent has a very strong reproductive capacity and can reproduce rapidly in sewage and occupy a dominant position. This rapid reproduction characteristic enables the microorganisms to form a large biological community in a relatively short period of time, thereby effectively treating pollutants in sewage.

[0033] Fast metabolism: Microorganisms have a fast metabolism and can produce a variety of degradation enzymes. Under the action of microorganisms, these degradation enzymes can efficiently degrade organic matter in sewage and convert it into harmless substances such as carbon dioxide and water.

[0034] Synergistic effect of multiple microorganisms: There are various types of microorganisms in microbial agents, including multiple microorganisms that can degrade different pollutants. These microorganisms can cooperate with each other and gradually decompose complex organic matter in sewage into simple inorganic matter through different metabolic pathways. This synergistic effect makes microbial agents more efficient and stable in treating complex sewage.

[0035] Transformation ability of special microorganisms: Some special microbial strains can transform or degrade a variety of toxic substances, such as sulfur-containing and ammonia-containing gases, formaldehyde, benzene and other toxic substances. These microorganisms transform these harmful substances into harmless and odorless substances through adsorption, absorption and metabolism, thereby achieving the purpose of purifying water quality and improving environmental hygiene.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] The present invention uses a water self-purification device and the addition of biological agents to utilize the metabolic action of microorganisms to remove pollutants such as organic matter in the water body, degrade indicators such as COD, BOD, ammonia nitrogen, and total phosphorus, inhibit the growth of cyanobacteria, and achieve self-purification and ecological restoration of the water quality of the bottom mud artificial lake, effectively solving the problems of cyanobacteria outbreaks and black and smelly water.

[0038] The mobile device is used in conjunction with the purification installation parts, so that it can move on the bottom of the lake. The raised guide rail is used to guide the raised guide rail, and the raised guide rail can be set according to the shape of the lake, which can effectively increase the comprehensiveness of water treatment and better target the purification of non-flowing water. The elastically mounted fitting groove wheel can realize elastic fitting and extrusion of the raised guide rail, which can be applied to the turning places of the raised guide rail, is more practical, can continuously move on the bottom of the lake, and perform steering guidance work.

[0039] By using liquid surface floating island parts, the water area can be aerated. At the same time, in conjunction with the lifting spray ring, biological bacteria or flocculants can be sprayed in the water area for purification. The isolation mesh used can reduce the diffusion of aeration and flocculation impurities and play an isolation role. By using the floating floating island box, the spray ring can be lifted and lowered according to the water level. A row of spacing adapter springs can be used to space the lifting and lowering spray rings, which can automatically adapt according to the water level to ensure the uniformity of the application of bacteria and drugs.

[0040] The double-layer suction piece can automatically absorb and clean impurities on the lake bottom, improve the cleaning effect of lakes without bottom mud, and prevent silt accumulation from affecting the lake ecology. The inner tube is combined with the anti-adsorption sleeve and the rubber structure, which is not easy to damage aquatic plants. The double-layer structure also prevents aquatic plants from clogging the anti-adsorption sleeve and the inner tube, and is not easily squeezed and bent, thereby improving the stability of impurity absorption. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 Schematic diagram of the placement of a floating island in a bottomless mud lake in Example 1 of the present invention

[0042] Figure 2 It is a schematic diagram of the overall structure of a water quality self-purification device for treating a bottomless mud lake according to the present invention;

[0043] Figure 3 This is a schematic diagram of the installation position of the mobile device of the present invention;

[0044] Figure 4 It is a schematic diagram of the bottom structure of the mobile device of the present invention;

[0045] Figure 5 For the present invention Figure 3 A magnified view of the structure of the middle A area;

[0046] Figure 6 This is a cross-sectional view of the purification installation structure of the present invention;

[0047] Figure 7 It is an enlarged schematic diagram of the structure of the mobile device of the present invention;

[0048] Figure 8 This is a schematic diagram of the structure of the fish driving control component of the present invention;

[0049] Fig. 9 This is a schematic diagram of the structure of the bacterial agent adding part of the present invention;

[0050] Fig.10 This is a cross-sectional view of the brushing auxiliary component structure of the present invention;

[0051] Fig.11 This is a cross-sectional view of the structure of the liquid surface floating island member of the present invention;

[0052] Fig.12 This is a schematic diagram of the installation position of the brushing auxiliary component of the present invention;

[0053] Fig.13 For the present invention Fig.12 Enlarged view of the structure of the middle G region.

[0054] In the figure: 1. Purification installation part; 101. Guide installation seat; 1011. Raised guide rail; 102. Fish driving seat; 103. Underwater buzzer speaker; 2. Moving device; 201. Moving bracket; 202. Moving extrusion rod; 203. Waterproof driving motor; 204. Driving groove wheel; 205. Driven wheel frame; 206. Spring shaft; 207. Fitting matching groove wheel; 3. Fish driving control part; 301. Control installation cylinder; 302. Down pressure head; 303. Delay piezoelectric switch; 4. Bacterial agent addition Part; 401, lifting installation column; 402, lifting spray ring; 403, spacing adaptation spring; 5, liquid surface floating island part; 501, floating island box; 502, isolation mesh; 5021, counterweight lead ring; 503, water filter net; 504, bacterial agent box; 505, water pump; 506, aeration pump; 507, suction pump; 6, brushing auxiliary part; 601, brushing rotating ring; 602, cleaning brush; 7, double-layer suction part; 701, double-layer suction main pipe; 702, inner pipe; 703, anti-adsorption sleeve. DETAILED DESCRIPTION

[0055] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0056] Embodiment 1: A biological agent for the treatment of a bottomless lake, the biological agent being composed of the following formula: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria and 10-60 parts of yeast.

[0057] Adding auxiliary ingredients: Adding appropriate amounts of nutrients, trace elements, stabilizers and protective agents to the biological agent. These ingredients can improve the activity and stability of the agent, extend the shelf life of the agent, and enhance the agent's ability to degrade pollutants. The added nutrients include, but are not limited to: carbon sources, nitrogen sources, phosphorus sources, inorganic salts, trace elements. The added stabilizers include at least one of glycerol, gelatin and lactitol. The added protective agents include at least one of sodium alginate, glycerol and β-cyclodextrin.

[0058] Equipment used: sterile operating table, constant temperature incubator, shaker, centrifuge, spectrophotometer and other experimental equipment to ensure sterile operation and precise control of the experimental process.

[0059] The role of bacteria:

[0060] (1) Bacillus subtilis

[0061] Function: Bacillus subtilis plays an important role in sewage treatment. It is an aerobic bacteria that can effectively decompose organic matter, reduce the chemical oxygen demand (COD) and biochemical oxygen demand (BOD) in sewage, and use enzymes to decompose complex organic matter in sewage and improve the biodegradability of sewage. At the same time, Bacillus subtilis can inhibit the growth of harmful bacteria by competing and producing antibacterial substances, thereby improving water quality.

[0062] (2) Photosynthetic bacteria

[0063] Function: Photosynthetic bacteria are a type of bacteria that can perform photosynthesis. They can use light energy, carbon dioxide and small organic molecules to grow and reproduce. In sewage treatment, photosynthetic bacteria can degrade pollutants such as organic matter, ammonia nitrogen and nitrite, while also inhibiting the growth of harmful algae and improving the transparency and self-purification ability of water bodies.

[0064] (3) Nitrifying bacteria

[0065] Function: Nitrifying bacteria are mainly responsible for converting ammonia nitrogen in sewage into nitrite and then into nitrate, which is a key step in the nitrogen cycle in sewage treatment. Through nitrification, ammonia nitrogen in sewage can be removed, thereby reducing the toxicity of water to aquatic organisms.

[0066] (4) Denitrifying bacteria

[0067] Function: Denitrifying bacteria are responsible for further reducing the nitrate produced by nitrifying bacteria into nitrogen gas and releasing it into the air, thus completing the complete removal of nitrogen. This process is of great significance for reducing eutrophication of water bodies and preventing black and smelly water bodies.

[0068] (5) Thiobacillus

[0069] Function: Thiobacillus is a type of bacteria that can oxidize sulfide and produce sulfuric acid. In the treatment of wastewater containing sulfide, Thiobacillus can play an important role in removing sulfide in wastewater through oxidation, thereby reducing its toxicity. In addition, Thiobacillus can also work synergistically with other microorganisms to promote the degradation of other pollutants in wastewater.

[0070] (6) Anaerobic ammonium oxidizing bacteria:

[0071] Function: Anaerobic ammonium oxidizing bacteria are bacteria that can directly convert ammonia nitrogen and nitrite into nitrogen gas under anaerobic conditions. This process does not require oxygen and organic carbon sources, so it has high energy efficiency and environmental value. In sewage treatment, anaerobic ammonium oxidizing bacteria can be used as an effective means of denitrification to reduce nitrogen emissions.

[0072] (7) Phosphate-solubilizing bacteria:

[0073] Function: Phosphate-solubilizing bacteria are a type of bacteria that can decompose organic and inorganic phosphorus compounds and release soluble phosphorus. In sewage treatment, phosphate-solubilizing bacteria can decompose phosphorus sources in sewage and increase the availability of phosphorus in water, thereby helping to remove phosphorus pollution. In addition, phosphate-solubilizing bacteria can form a symbiotic relationship with plant roots, promoting the absorption and utilization of phosphorus by plants.

[0074] (8) Sulfate-reducing bacteria:

[0075] Function: Sulfate-reducing bacteria are a type of bacteria that can reduce sulfate to sulfide under anaerobic conditions. In sewage treatment, they can remove sulfate from water through this process, thereby reducing the acidity of the water and the toxicity of heavy metals. In addition, sulfate-reducing bacteria can also work synergistically with denitrifying bacteria to improve the denitrification effect of sewage.

[0076] (9) Lactic Acid Bacteria (LAB):

[0077] Function: Although lactic acid bacteria are not the mainstream bacteria in sewage treatment, they can also play a certain role under certain conditions. For example, in a low temperature or low pH environment, lactic acid bacteria can secrete organic acids such as lactic acid to lower the pH value of sewage, thereby inhibiting the growth of harmful microorganisms and promoting the degradation of organic matter.

[0078] (10) Yeasts:

[0079] Function: Yeast is mainly used as a bioflocculant or biosorbent in sewage treatment. It can absorb and remove pollutants such as suspended solids and heavy metal ions in sewage, improving the purification effect of sewage. In addition, yeast can also secrete some biologically active substances, such as enzymes and antibiotics, which have an impact on the microbial community in sewage.

[0080] These strains can work together to promote the decomposition of organic matter, the removal of nitrogen and phosphorus, and the inhibition of cyanobacteria. The culture solution can be used as a stabilizer and protective agent to a certain extent to ensure the activity and stability of the bacterial agent during storage and use.

[0081] A method for preparing a biological agent for treating a bottomless lake comprises the following steps:

[0082] S1. Activation culture: inoculate the preserved strains into fresh culture medium for composite activation culture. Observe the growth of the composite strains under suitable temperature and light conditions to ensure that the composite strains are reactivated and in a good growth state.

[0083] S2. Expanded culture: The activated strains are inoculated into a larger culture container for expanded culture. The culture conditions (such as temperature, pH value, dissolved oxygen, etc.) are controlled to promote the rapid growth and reproduction of the strains. During the culture process, samples are taken regularly to test the concentration and activity of the strains to ensure that the growth of the strains meets the requirements.

[0084] S3, homogenization treatment: by stirring or oscillating, the mixed strains and auxiliary ingredients are fully mixed to form a homogenized microbial agent. The homogenization treatment can ensure that the components in the microbial agent are evenly distributed and improve the use effect of the microbial agent;

[0085] S4. Packaging: The prepared microbial agent is packaged. The packaging container should be made of sterile and well-sealed materials, such as glass bottles and plastic bottles. During the packaging process, care should be taken to avoid contamination and leakage to ensure the purity and activity of the microbial agent.

[0086] S5. Storage: Store the packaged bacterial agent in a cool, dry, dark place to avoid direct sunlight and high temperature environment which may have adverse effects on the bacterial agent. At the same time, regularly check the storage condition of the bacterial agent to ensure that the bacterial agent maintains good activity and stability within the validity period.

[0087] refer to Figure 1 In this embodiment, a method of adding the biological agent for the treatment of the bottomless lake can be adopted, which is artificial placement. The method of using the biological agent for the treatment of the bottomless lake is as follows:

[0088] Dosage calculation

[0089] The dosage of bacterial agent should be reasonably calculated according to the scale of the treatment system, water quality and treatment objectives. Generally speaking, the dosage of bacterial agent for domestic sewage is about 1% - 30% (i.e. 10L - 300L per cubic meter), and the dosage of bacterial agent for industrial sewage is 10% - 45% (i.e. 100L - 450L per cubic meter).

[0090] Dosing point and time

[0091] Select appropriate locations in the artificial lake to set up dosing points (such as the center of the regional lake surface, around the floating island or at the intersection of water flows), and determine the dosing time and amount based on water quality monitoring results and treatment needs.

[0092] Dosing method

[0093] According to the water quality monitoring results, biological agents are regularly added to the lake. During the normal operation of the system, in order to maintain the activity and quantity of the bacteria, a batch addition method can be adopted, that is, the bacteria are divided into several batches and gradually added to the system over a period of time. Batch addition can reduce the shock load of the bacteria in the system, allow the bacteria to gradually adapt to the new environment, and help improve the treatment effect.

[0094] Direct dosing method: When re-dosing the bacterial agent at system startup or after overhaul, a one-time dosing method can be adopted, that is, the calculated total amount of the bacterial agent is added to the system at one time, and the bacterial agent is directly added to a specific location of the sewage treatment system, such as the water inlet of an anaerobic tank, aerobic tank or bioreactor. This method is simple to operate, but it should be noted that the bacterial agent may lose some activity due to water impact during the dosing process.

[0095] Dilution dosing method: Mix and dilute the bacterial agent with an appropriate amount of clean water or sewage, and then add it to the treatment system. The dilution dosing method can reduce the loss of the bacterial agent during the dosing process and help to evenly distribute the bacterial agent in the system.

[0096] Activation dosing method: For some bacterial agents that require specific environmental conditions, activation treatment can be carried out first. That is, the bacterial agent, nutrient source (such as glucose, urea, potassium dihydrogen phosphate, etc.) and water are mixed in a certain proportion and activated under suitable temperature and conditions. The activated bacterial agent has higher activity and can better adapt to the environment of the treatment system after dosing, thereby improving the treatment efficiency.

[0097] Continuous dosing: For some treatment systems that need to maintain a high bacterial concentration, a continuous dosing method can be used. That is, the bacterial agent is continuously dosed into the system at a constant flow rate through a special dosing device. This method can ensure the continuous supply and stable activity of the bacterial strain in the system, but attention should be paid to the precise control of the dosing amount and the stable operation of the dosing device.

[0098] Maintenance Management

[0099] 1. Regular inspection and monitoring

[0100] During the process of adding bacterial agents, water quality indicators and treatment effects should be monitored regularly, and the dosage and environmental conditions should be adjusted in time according to the monitoring results to ensure stable operation and efficient treatment of the treatment system.

[0101] Monitor changes in water quality indicators (such as COD, BOD, ammonia nitrogen, total phosphorus, etc.), evaluate treatment effects, and adjust treatment plans in a timely manner.

[0102] Regularly inspect and maintain the biological island, aeration equipment and bacterial agent addition points to ensure their normal operation and effective governance.

[0103] 2. Cleaning and repair

[0104] Regularly clean the plant residues and impurities in the biological fillers on the floating island to keep the floating island clean and promote plant growth.

[0105] 3. Supplementation and adjustment of microbial agents

[0106] According to the water quality monitoring results and treatment effects, the type and dosage of bacterial agents should be supplemented or replaced in a timely manner.

[0107] Adjust aeration intensity and operation time to adapt to water quality changes and treatment needs.

[0108] Advantages of microorganisms

[0109] 1. Strong environmental adaptability: The microorganisms in the microbial agent have strong environmental adaptability, are prone to mutation after being affected by environmental factors, and can form a rapid and effective material exchange with the surrounding environment. This enables the microbial agent to function under different water quality conditions, including changes in environmental factors such as temperature, pH value, and dissolved oxygen.

[0110] 2. Low temperature resistance and impact resistance: Some special microbial agents also have the characteristics of low temperature resistance and impact resistance. Even in low temperature or large fluctuations in water quality, these microorganisms can still maintain high activity and degradation ability to ensure the effectiveness of sewage treatment.

[0111] 3. Aerobic and anaerobic treatment: In practical applications, microbial agents can treat sewage in both aerobic and anaerobic ways. Under aerobic conditions, microorganisms oxidize and decompose organic matter into carbon dioxide and water through catabolism and anabolism; while under anaerobic conditions, microorganisms can decompose macromolecular organic matter into soluble substances, which are then further degraded into low molecular organic acids and alcohols by specific anaerobic bacteria, and finally produce gases such as methane.

[0112] 4. In-situ remediation: Use microbial agents to treat water bodies without pumping out the water. After the agents are added, they can rapidly proliferate and degrade pollutants in the water body, restoring the self-purification capacity of the water body in a short period of time. This in-situ remediation method not only saves time and cost, but also avoids secondary pollution that may occur during the transportation and treatment of the water body.

[0113] Principles of sewage treatment

[0114] Biological islands replace bottom mud to carry microorganisms: biological fillers for microorganisms to attach and grow, such as ecological expanded clay, activated carbon, etc., are selected to provide a living environment for probiotics.

[0115] Strong reproductive capacity: The microbial population in the microbial agent has a very strong reproductive capacity and can reproduce rapidly in sewage and occupy a dominant position. This rapid reproduction characteristic enables the microorganisms to form a large biological community in a relatively short period of time, thereby effectively treating pollutants in sewage.

[0116] Fast metabolism: Microorganisms have a fast metabolism and can produce a variety of degradation enzymes. Under the action of microorganisms, these degradation enzymes can efficiently degrade organic matter in sewage and convert it into harmless substances such as carbon dioxide and water.

[0117] Synergistic effect of multiple microorganisms: There are various types of microorganisms in microbial agents, including multiple microorganisms that can degrade different pollutants. These microorganisms can cooperate with each other and gradually decompose complex organic matter in sewage into simple inorganic matter through different metabolic pathways. This synergistic effect makes microbial agents more efficient and stable in treating complex sewage.

[0118] Transformation ability of special microorganisms: Some special microbial strains can transform or degrade a variety of toxic substances, such as sulfur-containing and ammonia-containing gases, formaldehyde, benzene and other toxic substances. These microorganisms transform these harmful substances into harmless and odorless substances through adsorption, absorption and metabolism, thereby achieving the purpose of purifying water quality and improving environmental hygiene.

[0119] The test data of a hotel in Beijing is as follows

[0120]

[0121] Embodiment 2:

[0122] See also Figure 2 - Fig.13 As shown:

[0123] The present invention provides a technical solution: a water quality self-purification device for the treatment of a bottomless lake, comprising a purification installation part 1, a moving device 2 is installed on the purification installation part 1, and the moving device 2 is used to move and adjust the purification position; a row of fish-driving control parts 3 are installed on the purification installation part 1; the fish-driving control parts 3 are used to drive away the fish school; a bacterial agent adding part 4 is installed on the moving device 2; the bacterial agent adding part 4 is used to spray biological bacterial agents in the lake water, and the bacterial agent adding part contains biological bacterial agents for the treatment of the bottomless lake; a liquid surface floating island part 5 is installed on the bacterial agent adding part 4; the liquid surface floating island part 5 is used to aerate the lake water; a brush is installed on the moving device 2 A wiping auxiliary part 6, and the brushing auxiliary part 6 is used to clean the liquid surface floating island part 5; a double-layer suction part 7 is installed on the moving device 2; the double-layer suction part 7 is used to prevent damage to aquatic plants; the purification installation part 1 includes: a guide mounting seat 101 and a raised guide rail 1011, and a row of through holes are respectively provided on both sides of the guide mounting seat 101; the through holes on both sides of the guide mounting seat 101 are used to pass bolts to install the guide mounting seat 101 on the bottom of the water; a raised guide rail 1011 is fixedly installed on the guide mounting seat 101, and the guide mounting seat 101 is composed of two round steel columns; the raised guide rail 1011 is used to guide the movement of the moving device 2.

[0124] Wherein, the purification installation part 1 also includes: a fish-driving seat 102 and an underwater buzzer speaker 103, a row of fish-driving seats 102 are fixedly installed on the side of the guide installation seat 101 by bolts, and underwater buzzer speakers 103 are fixedly installed on a row of guide installation seats 101; the underwater buzzer speakers 103 are used to drive away fish; the moving device 2 includes: a moving bracket 201, a moving extrusion rod 202, a waterproof driving motor 203, a driving groove wheel 204, a driven wheel frame 205, a spring shaft 206 and a fitting matching groove wheel 207, and the driving groove wheel 204 and the fitting matching groove wheel 207 are respectively provided with grooves for adapting to the raised guide rail 1011; the top of the moving bracket 201 is a circular structure; the moving bracket 201 is located outside the raised guide rail 1011; a moving extrusion rod 202 is fixedly installed on the bottom of the moving bracket 201, and the bottom of the moving extrusion rod 202 is an arc structure; Two waterproof driving motors 203 are installed; driving groove wheels 204 are fixedly installed on the output shafts of the two waterproof driving motors 203, and the ends of the two driving groove wheels 204 are rotatably installed on the mobile bracket 201; the two driving groove wheels 204 are attached to the raised guide rail 1011; a circle of meshing teeth are respectively provided on the top of the two driving groove wheels 204; two driven wheel frames 205 are attached to the inner side of the mobile bracket 201; two spring shafts 206 are respectively fixedly installed on the two driven wheel frames 205, and the four spring shafts 206 are respectively slidably inserted on the mobile bracket 201; springs are respectively sleeved on the four spring shafts 206; two fitting matching groove wheels 207 are respectively rotatably installed on the two driven wheel frames 205, and the two fitting matching groove wheels 207 are respectively elastically attached to the raised guide rail 1011; the driving groove wheels 204 and the fitting matching groove wheels 207 are used for rolling on the raised guide rail 1011;The mobile device 2 is used in conjunction with the purification installation part 1, so that it can move on the bottom of the lake. The raised guide rail 1011 is used to guide, and the raised guide rail 1011 can be set according to the shape of the lake, which can effectively increase the comprehensiveness of water treatment and better target the purification of non-flowing water. The driving groove wheel 204 is used in conjunction with the fitting groove wheel 207, and can be clamped on the raised guide rail 1011 for movement. The elastically installed fitting groove wheel 207 can realize elastic fitting and squeezing of the raised guide rail 1011, and can be suitable for the bending part of the raised guide rail 1011, which is more practical, can continuously move on the bottom of the lake, and perform steering guidance work. The guide mounting seat 101 is placed on the bottom of a lake without bottom mud and installed by bolts. According to the actual lake area and shape, the guide mounting seat 101 and the raised guide rail 1011 are produced. The raised guide rail 1011 can be bent in an "S" shape to adapt to the shape of the lake. The waterproof driving motor 203 drives the driving groove wheel 204 to rotate, which can drive the mobile bracket 201 to move at the bottom of the lake. Under the strong squeezing of the spring on the spring shaft 206, the fitting groove wheel 207 can be elastically rolled and fitted at any position on the raised guide rail 1011, ensuring that the mobile bracket 201 can move and operate normally. The cylindrical structure of the raised guide rail 1011 also ensures that the driving groove wheel 204 and the fitting groove wheel 207 can use their own grooves to fit and limit to prevent falling off. ;

[0125] The fish-driving control component 3 includes: a control installation tube 301, a pressure head 302 and a delayed piezoelectric switch 303. The GE-320A underwater buzzer speaker 103 can be used to disperse the fish school. The delayed piezoelectric switch 303 can be used with a micro switch in conjunction with a LADT2 delay module. The control installation tube 301 is fixedly sleeved on the fish-driving seat 102; the control installation tube 301 is aligned with the movable extrusion rod 202; the control installation tube 301 is slidably plugged with a pressure head 302, and the top of the pressure head 302 is a hemispherical structure; A spring is provided at the bottom of the pressure head 302, and the spring provided at the bottom of the lower pressure head 302 is sleeved inside the control installation tube 301; the mobile squeezing rod 202 is used to move and squeeze the lower pressure head 302; a delayed piezoelectric switch 303 is fixedly installed inside the control installation tube 301, and the delayed piezoelectric switch 303 is located at the bottom of the lower pressure head 302; the delayed piezoelectric switch 303 is electrically fused to the underwater buzzer speaker 103; the fish-repelling control part 3 is adopted, and the mobile bracket 201 can be used to move and apply medicine to drive the mobile squeezing rod 202 to automatically control the fish-repelling The work of the fish school improves the safety of the actual lake water purification treatment, and can effectively prevent the problem that the medicine is not completely diluted and mixed when the lifting spray ring 402 just sprays out the medicine, the concentration is too high, and it is easy to cause irreversible damage to the fish. This structure does not need to drive away the fish school in the entire lake area, and can be automatically controlled on the moving route of the raised guide rail 1011. At the same time, this structure uses the delayed piezoelectric switch 303, which has a delay function and pre-sets the time to ensure that the medicine is fully mixed and diluted, and automatically turns off the underwater buzzer after the water quality is purified. The device 103 ensures a normal growth environment for fish, can ensure the range of fish activities, and can prevent high concentrations of drugs from damaging fish. When the mobile bracket 201 moves, it drives the mobile squeezing rod 202 to squeeze the pressing head 302. After the hemispherical top of the pressing head 302 is squeezed by the arc-shaped bottom of the mobile squeezing rod 202, the pressing head 302 moves downward to press the delayed piezoelectric switch 303. At this time, the delayed piezoelectric switch 303 can be energized to control the underwater buzzer speaker 103 to play noise to drive away the fish. At this time, the fish will not approach the drug delivery area.

[0126] The microbial agent adding part 4 includes: a lifting installation column 401, a lifting spray ring 402 and a spacing adaptation spring 403. The lifting installation column 401 is fixedly installed on the mobile bracket 201; the upper section of the lifting installation column 401 is a hexagonal column structure; a row of lifting spray rings 402 are slidably sleeved on the lifting installation column 401; a circle of nozzles are respectively arranged on the outer side of a row of lifting spray rings 402; a row of lifting spray rings 402 are connected by a hose; a row of lifting spray rings 402 are connected by a spacing adaptation spring 403. , and a row of spacing adaptation springs 403 are respectively sleeved on the lifting installation column 401; a row of spacing adaptation springs 403 have the same elastic force; a row of spacing adaptation springs 403 are used to separate a row of lifting spray rings 402; the liquid surface floating island part 5 includes: a floating island box 501, an isolation mesh 502, a counterweight lead ring 5021 and a water filter net 503, and the counterweight lead ring 5021 at the bottom is connected to the side of the mobile extrusion rod 202 and the mobile bracket 201 through two steel wires; a foam float is provided at the bottom of the floating island box 501, refer to Figure 1, the floating island box 501 can be installed on the floating island in the center, and the other peripheral floating islands can be removed; the floating island box 501 is slidably plugged into the hexagonal column on the upper section of the lifting installation column 401; an isolation mesh 502 is fixedly installed at the bottom of the floating island box 501; a row of weighted lead rings 5021 are fixedly installed on the isolation mesh 502; the floating island box 501 is used to float on the liquid surface; the weighted lead rings 5021 are used to pull down the isolation mesh 502; a water filter net 503 is fixedly installed on the floating island box 501; the water filter net 503 is used to isolate impurities; the floating island on the liquid surface Component 5 also includes: a bacterial agent box 504, a water pump 505, an aeration pump 506 and a suction pump 507. The bacterial agent box 504 is fixedly installed on the floating island box 501; an opening is provided at the top of the bacterial agent box 504; a water pump 505 is fixedly installed at the bottom of the bacterial agent box 504, and the water pump 505 passes through the floating island box 501; the water outlet pipe of the water pump 505 is connected to the lifting liquid spray ring 402 above; an aeration pump 506 is fixedly installed on the top of the floating island box 501, and the jet pipe of the aeration pump 506 passes through the floating island box 501, and the jet pipe of the aeration pump 506 is located at The suction pump 507 is fixedly mounted on the floating island box 501; the suction pump 507 is used to suck the precipitated impurities. The liquid surface floating island 5 can be used to aerate the water area. At the same time, the lifting liquid spray ring 402 can be used to spray biological bacteria or flocculation agents in the water area to perform purification treatment. The isolation mesh 502 can reduce the diffusion of aeration and flocculation impurities and play an isolation role. The floating floating island box 501 can be used to press the lifting liquid spray ring 402 according to the water level to ensure that a row of The lifting and lowering spray rings 402 are all located in the water, and a row of spacing adaptation springs 403 are used to space the lifting and lowering spray rings 402, which are automatically adapted according to the water level to ensure the uniformity of the application of the bacterial agent. When the floating island box 501 is lifted and floated, the upper hexagonal column of the lifting and lowering mounting column 401 can limit the floating island box 501 to only slide vertically, preventing the isolation mesh 502 from rotating and affecting the subsequent brushing of the brushing auxiliary part 6. The counterweight lead ring 5021 can be used to pull down the isolation mesh 502 for adaptation to improve the vertical effect of the isolation mesh 502.

[0127] It should be noted that the floating island box 501 is made of environmentally friendly, weather-resistant and buoyant materials, such as HDPE (high-density polyethylene) floating plates, to ensure that the floating island can float stably in the water and can be connected to the surrounding Figure 1 Used in conjunction with the central floating island.

[0128] According to the area, shape and pollution of the artificial lake, the layout and number of floating islands are reasonably designed to ensure the coverage area and purification effect. In principle, the design should be 1%-15% of the total lake area, with a minimum size of 4m*4m.

[0129] At the same time, a small biological filler (not shown in the figure) can be installed at the bottom of the floating island box 501. The biological filler is a biological filler suitable for the attachment and growth of microorganisms, such as ecological ceramsite, activated carbon, etc., to provide a living environment for the probiotics.

[0130] At the same time, plants (not shown in the figure) can also be planted on the outer plates of the floating island box 501. Plant selection: According to the local climate and water quality conditions, suitable ornamental plants such as lotus, water grass, reed, cattail, etc. can be selected, while considering the water purification ability and ecological effect of the plants.

[0131] The floating island box 501, biological filler and plants are assembled into a complete floating island unit according to the design requirements. A biological island consisting of the floating island box 501 and biological filler (such as ecological ceramsite, activated carbon, etc.) is arranged in the artificial lake, and ornamental plants such as lotus and water grass are planted on the island, which not only beautifies the environment but also provides a matrix for microorganisms to attach and grow.

[0132] Embodiment 3, on the basis of embodiment 2, the brushing auxiliary part 6 comprises: a brushing rotating ring 601 and a cleaning brush 602, the brushing rotating ring 601 is rotatably sleeved on the mobile bracket 201; a gear is provided on the inner side of the brushing rotating ring 601, and the gear on the inner side of the brushing rotating ring 601 is meshed with the meshing teeth on the top of the two driving groove wheels 204; a cleaning brush 602 is fixedly installed on the side of the brushing rotating ring 601, and a row of elastic soft bristles is provided on the cleaning brush 602; the row of elastic soft bristles provided on the cleaning brush 602 is used to brush Wipe the isolation mesh 502; the double-layer suction member 7 includes: a double-layer suction main pipe 701 and an inner pipe 702, the double-layer suction main pipe 701 is fixedly installed on the mobile bracket 201 through a bracket; a row of inner pipes 702 are fixedly installed on the double-layer suction main pipe 701, and the outer side of the inner pipe 702 is provided with absorption holes; the inner pipe 702 is a rubber hose; the double-layer suction main pipe 701 is connected to the water inlet of the suction pump 507 through a hose; the double-layer suction member 7 also includes: an anti-adsorption sleeve 703, fixedly installed on the double-layer suction main pipe 701 There is a row of anti-adsorption sleeves 703, and the row of anti-adsorption sleeves 703 are respectively located outside the row of inner tubes 702; the anti-adsorption sleeves 703 are rubber hoses; the anti-adsorption sleeves 703 are used to reduce damage to aquatic plants, and the brushing auxiliary part 6 is used to automatically brush the isolation mesh 502 to assist in improving the isolation effect of the isolation mesh 502. The automatic control has a simple and reasonable structure, which can prevent the isolation mesh 502 from being blocked as a whole and causing poor water circulation. The double-layer suction part 7 can automatically suck impurities from the lake bottom. It can perform absorption and cleaning work, improve the cleaning effect of lakes without bottom mud, prevent silt accumulation from affecting the lake ecology, use the inner tube 702 in conjunction with the anti-absorption sleeve 703, and the rubber structure, which is not easy to damage aquatic plants. The double-layer structure also prevents aquatic plants from clogging the anti-absorption sleeve 703 and the inner tube 702, and is not easy to be squeezed and bent, thereby improving the stability of absorbing impurities and being more suitable for underwater environments. Part of the precipitated flocculants and part of the silt can be absorbed and discharged into the floating island box 501, filtered through the filter water net 503, and then continued to be discharged into the lake water for circulation.

[0133] The working principle of this embodiment is as follows: first, the guide mounting seat 101 is placed at the bottom of a lake without bottom mud, and is fixed by bolts. According to the actual area and shape of the lake, the guide mounting seat 101 and the raised guide rail 1011 are produced. The raised guide rail 1011 can have an "S" shape to adapt to the shape of the lake. The driving groove wheel 204 set by the waterproof driving motor 203 is driven to rotate, so as to drive the mobile bracket 201 to move at the bottom of the lake; when the mobile bracket 201 moves, it drives the mobile squeezing rod 202 to squeeze the pressing head 302. After the hemispherical top of the pressing head 302 is squeezed by the arc-shaped bottom of the mobile squeezing rod 202, the lower pressing head 302 is The pressure head 302 moves downward to press the delayed piezoelectric switch 303. At this time, the delayed piezoelectric switch 303 can be energized to control the underwater buzzer speaker 103 to play the noise to drive away the fish. At this time, the fish will not approach. At this time, the water pump 505 extracts the bacteria or medicine in the bacteria box 504 for spraying. The bacteria or medicine gradually mixes in this area for purification. Flocculants can be used to assist in flocculating impurities to improve the water quality of the lake. Biological bacteria can improve the self-cleaning ability of the lake water. The flocculated impurities are isolated inside the isolation mesh 502 and flocculate downward and settle on the bottom of the lake. As the mobile bracket 201 continues to move, the downward pressure head 302 that has passed is pressed again to control the corresponding water The buzzer speaker 103 drives away the fish school. As time goes by, the medicine is fully mixed with the lake water and diluted. The delayed piezoelectric switch 303 is automatically powered off to prevent the underwater buzzer speaker 103 from continuously making sounds and affecting the activities of fish. As the mobile bracket 201 moves, the floating island box 501 floats on the water surface. The floating island box 501 presses the lifting spray ring 402, and a row of spacing adapter springs 403 are compressed, automatically spacing a row of lifting spray rings 402. The bacterial agent box 504 is opened at the top, and biological bacterial agents are added inside. The water pump 505 draws the biological bacterial agents and discharges them through the lifting spray ring 402. The air can be filled into the water through the aeration pump 506 for aeration. The air works, and the impurities are flocculated by the agent and precipitated at the bottom of the lake; when the mobile bracket 201 moves, the double-layer suction main pipe 701 is driven to move at the same time, and the inner pipe 702 and the anti-adsorption sleeve 703 are elastically attached to the bottom of the water. The suction is sucked by the suction pump 507, and part of the precipitated flocculants and part of the silt can be absorbed and discharged into the floating island box 501. After being filtered by the filter water net 503, they are continuously discharged into the lake water for circulation to maintain the effect of the lake bottom sludge pollution. When the waterproof driving motor 203 drives the driving groove wheel 204 to rotate and move, the meshing teeth on the driving groove wheel 204 mesh with the driving brush wiping rotating ring 601 to rotate, and the cleaning brush 602 is controlled to rotate and brush the isolation mesh cloth 502 to perform cleaning work.

[0134] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or includes elements inherent to such process, method, article or device.

[0135] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological agent for treating a bottomless lake, characterized in that: The biological bacterial agent includes the following raw materials in parts by weight: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria and 10-60 parts of yeast.

2. The biological agent for treating a bottomless lake according to claim 1, characterized in that: The biological agent also includes: nutrients, trace elements, stabilizers and protective agents.

3. The biological agent for treating a bottomless lake according to claim 2, characterized in that: The nutrients include at least one of a carbon source, a nitrogen source, a phosphorus source, an inorganic salt and a trace element.

4. The biological agent for treating a bottomless lake according to claim 2, characterized in that: The stabilizer includes at least one of glycerin, gelatin and lactitol.

5. The biological agent for treating a bottomless lake according to claim 2, characterized in that: The protective agent includes at least one of sodium alginate, glycerol and β-cyclodextrin.

6. A method for preparing a biological agent for treating a bottomless lake according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Activation culture: inoculate the preserved bacteria into fresh culture medium for composite activation culture; S2. Expanded culture: The activated strains are inoculated into a larger culture container for expanded culture. The culture conditions are controlled to promote the rapid growth and reproduction of the strains. During the culture process, samples are taken regularly to test the concentration and activity of the strains to ensure that the growth of the strains meets the requirements. S3, homogenization treatment: by stirring or oscillating, the mixed bacteria and nutrients, trace elements, stabilizers and protective agents are fully mixed to form a homogenized bacterial agent; S4. Packaging: The prepared bacterial agent is packaged. The packaging container should be made of sterile and well-sealed materials, such as glass bottles and plastic bottles; S5. Storage: Store the packaged microbial agent in a cool, dry, dark place. At the same time, check the storage condition of the microbial agent regularly.

7. A water quality self-purification device for treating a bottomless lake comprising the biological bacterial agent for treating a bottomless lake as claimed in any one of claims 1 to 5, characterized in that: The invention comprises a purification installation part (1), on which a moving device (2) is installed, and the characteristics are as follows: the moving device (2) is used to move and adjust the purification position; a row of fish-driving control parts (3) are installed on the purification installation part (1); the fish-driving control parts (3) are used to drive away fish schools; The mobile device (2) is provided with a microbial agent adding unit (4); the microbial agent adding unit (4) contains the biological microbial agent for treating a bottomless mud lake as claimed in claim 1; A liquid surface floating island component (5) is installed on the bacterial agent adding part (4); the liquid surface floating island component (5) is used to aerate the lake water; The moving device (2) is provided with a brushing auxiliary part (6), and the brushing auxiliary part (6) is used to clean the liquid surface floating island part (5); A double-layer suction piece (7) is installed on the mobile device (2); the double-layer suction piece (7) is used to prevent damage to aquatic plants; The purification mounting component (1) comprises: a guide mounting seat (101) and a raised guide rail (1011), wherein a row of through holes are respectively provided on both sides of the guide mounting seat (101); the through holes on both sides of the guide mounting seat (101) are used to pass bolts to install the guide mounting seat (101) on the bottom of the water; the raised guide rail (1011) is fixedly mounted on the guide mounting seat (101), and the guide mounting seat (101) is composed of two round steel columns; the raised guide rail (1011) is used to guide the movement of the moving device (2).

8. The water quality self-purification device for bottomless mud lake treatment according to claim 7 is characterized by: The purification installation component (1) further comprises: a fish-repelling seat (102) and an underwater buzzer speaker (103); a row of fish-repelling seats (102) are fixedly mounted on the side of the guide installation seat (101) by bolts; and underwater buzzers (103) are fixedly mounted on a row of guide installation seats (101); the underwater buzzers (103) are used to drive away schools of fish; the moving device (2) comprises: a moving bracket (201), a moving extrusion rod (202), a waterproof driving motor (203), a driving groove wheel (204), a driven wheel frame (205), a spring shaft (206) and a fitting groove wheel (207); the top of the moving bracket (201) is a circular structure; the moving bracket (201) is located at a raised position. The movable bracket (201) is provided with a movable extrusion rod (202) fixedly mounted on the bottom of the movable bracket (201), and the bottom of the movable extrusion rod (202) is an arc-shaped structure; two waterproof driving motors (203) are fixedly mounted on the movable bracket (201); driving groove wheels (204) are respectively fixedly mounted on the output shafts of the two waterproof driving motors (203), and the ends of the two driving groove wheels (204) are respectively rotatably mounted on the movable bracket (201); the two driving groove wheels (204) are attached to the raised guide rail (1011); the tops of the two driving groove wheels (204) are respectively provided with a circle of meshing teeth; two driven wheel frames (205) are attached to the inner side of the movable bracket (201); the two driven wheels Two spring shafts (206) are fixedly mounted on the frame (205), and the four spring shafts (206) are respectively slidably plugged on the movable bracket (201); the four spring shafts (206) are respectively sleeved with springs; two driven wheel frames (205) are respectively rotatably mounted with two fitting groove wheels (207), and the two fitting groove wheels (207) are respectively elastically fitted on the raised guide rail (1011); the driving groove wheel (204) and the fitting groove wheel (207) are used to roll on the raised guide rail (1011), and the fish driving control component (3) comprises: a control installation cylinder (301), a lower pressure head (302) and a delayed piezoelectric switch (303), and the control installation cylinder (301) is fixed The control installation tube (301) is sleeved on the fish driving seat (102); the control installation tube (301) is aligned with the movable extrusion rod (202); a pressing head (302) is slidably inserted on the control installation tube (301), and the top of the pressing head (302) is a hemispherical structure; a spring is provided at the bottom of the pressing head (302), and the spring provided at the bottom of the pressing head (302) is sleeved inside the control installation tube (301); the movable extrusion rod (202) is used to move and extrude the pressing head (302); a time-delay piezoelectric switch (303) is fixedly installed inside the control installation tube (301), and the time-delay piezoelectric switch (303) is located at the bottom of the pressing head (302); the time-delay piezoelectric switch (303) is electrically fused and connected to the underwater buzzer speaker (103).

9. The water quality self-purification device for bottomless mud lake treatment according to claim 8 is characterized by: The bacterial agent adding part (4) comprises: a lifting installation column (401), a lifting liquid spraying ring (402) and a spacing adapting spring (403); the lifting installation column (401) is fixedly installed on the movable bracket (201); the upper section of the lifting installation column (401) is a hexagonal column structure; a row of lifting liquid spraying rings (402) are slidably sleeved on the lifting installation column (401); a circle of spray heads are respectively arranged on the outer side of a row of lifting liquid spraying rings (402); a row of lifting liquid spraying rings (402) are connected by a hose; a row of lifting liquid spraying rings (402) are provided with a spacing adapting spring (403), and a row of spacing adapting springs ( 403) are respectively sleeved on the lifting installation column (401); a row of spacing adaptation springs (403) have the same elastic force; a row of spacing adaptation springs (403) are used to space a row of lifting spray rings (402), the liquid surface floating island member (5) comprises: a floating island box (501), an isolation mesh (502), a counterweight lead ring (5021), a water filter net (503), a bacterial agent box (504) and a suction pump (507), and a foam floating block is provided at the bottom of the floating island box (501); the floating island box (501) is slidably plugged into the hexagonal column at the upper section of the lifting installation column (401); the bottom of the floating island box (501) is fixedly installed with an isolation mesh (502); a row of weighted lead rings (5021) are fixedly mounted on the isolation mesh (502); the weighted lead ring (5021) at the bottom is respectively connected to the side of the movable extrusion rod (202) and the movable bracket (201) through two steel wires; the floating island box (501) is used to float on the liquid surface; the weighted lead ring (5021) is used to pull down the isolation mesh (502); a water filter net (503) is fixedly mounted on the floating island box (501); the water filter net (503) is used to isolate impurities, and the bacterial agent box (504) is fixedly mounted on the floating island box (501); the top of the bacterial agent box (504) is provided with an opening, and the bacterial agent box (504) is provided with a plurality of holes. The agent box (504) is used to store the biological agent; a water pump (505) is fixedly installed at the bottom of the bacterial agent box (504), and the water pump (505) passes through the floating island box (501); the water outlet pipe of the water pump (505) is connected to the upper lifting spray ring (402); an aeration pump (506) is fixedly installed on the top of the floating island box (501), and the jet pipe of the aeration pump (506) passes through the floating island box (501), and the jet pipe of the aeration pump (506) is located inside the isolation mesh (502); the suction pump (507) is fixedly installed on the floating island box (501); the suction pump (507) is used to suck out precipitated impurities.

10. The water quality self-purification device for bottomless mud lake treatment according to claim 9 is characterized by: The brushing auxiliary part (6) comprises: a brushing rotating ring (601) and a cleaning brush (602), wherein the brushing rotating ring (601) is rotatably sleeved on the movable bracket (201); a gear is provided on the inner side of the brushing rotating ring (601), and the gear on the inner side of the brushing rotating ring (601) meshes with the meshing teeth on the top of the two driving groove wheels (204); a cleaning brush (602) is fixedly installed on the side of the brushing rotating ring (601), and a row of elastic soft bristles is provided on the cleaning brush (602); the row of elastic soft bristles provided on the cleaning brush (602) is used to brush the isolation mesh (502), and the double-layer suction part (7) comprises: a double-layer suction main pipe (701), an inner pipe (702) and an anti-sucking pipe (703). Adsorption sleeve (703), the double-layer suction main pipe (701) is fixedly installed on the mobile bracket (201) through a bracket; a row of inner tubes (702) is fixedly installed on the double-layer suction main pipe (701), and absorption holes are arranged on the outer side of the inner tube (702); the inner tube (702) is a rubber hose; the double-layer suction main pipe (701) is connected to the water inlet of the suction pump (507) through a hose, and a row of anti-adsorption sleeves (703) is fixedly installed on the double-layer suction main pipe (701), and a row of anti-adsorption sleeves (703) are respectively located outside a row of inner tubes (702); the anti-adsorption sleeves (703) are rubber hoses; the anti-adsorption sleeves (703) are used to reduce damage to aquatic plants.

Citation Information

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