Culture device for agricultural microbial agent production and use method

Abstract

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial cultivation, and in particular to a cultivation device for producing agricultural microbial agents and a use method thereof. Background Art

[0002] Microbial agents are active biological preparations made by industrially propagating specific functional microorganisms (such as bacteria, fungi, and actinomycetes) and combining them with carriers (such as peat, straw powder, and humic acid). Their core purpose is to utilize the metabolic activities of microorganisms to improve the soil environment or promote plant growth. For example, rhizobium agents can fix nitrogen from the air for use by legumes, while Bacillus subtilis agents can inhibit pathogens and secrete plant hormones. By improving soil fertility and enhancing crop resistance, these products can replace some chemical fertilizers and pesticides in green agriculture, achieving a balance between ecological and economic benefits.

[0003] In the prior art, for example, Chinese patent number CN113943637B discloses a microbial agent cultivation device in the field of microbial agent cultivation technology, comprising a nutrient solution tank, an inner wall of which is slidably connected to a mounting plate, on which a plurality of culture groups arranged front to back and equidistantly distributed are fixedly provided, and a linear drive mechanism is provided in the nutrient solution tank, which is used to drive the mounting plate to move the culture groups downward to absorb nutrient solution; the present invention can ensure that the elements in the nutrient solution are in full contact with the microbial agent, so that the microbial agent can grow better.

[0004] Although the above scheme has the advantages as mentioned above, the disadvantage of the above scheme is that although it can drive the mounting plate to move downward by the linear drive mechanism, the mounting plate will drive the incubator, the microbial agent, the liquid storage tank and the stirring fan blade push plate to move downward, and the stirring fan blade will fully stir the nutrient solution in the nutrient solution tank while moving downward, so that a large number of elements accumulated at the bottom of the nutrient solution tank can be evenly distributed in the nutrient solution. However, the nutrient solution tank is provided with a linear drive mechanism, a stirring and liquid adding mechanism and a first drive mechanism. The nutrient solution added to the nutrient solution tank will contact multiple components of the linear drive mechanism, the stirring and liquid adding mechanism and the first drive mechanism, making the cleaning of the nutrient solution tank more troublesome, and there may be a situation where the cleaning is not in place, so that the nutrient solution is easily contaminated when nutrient solution is added to the nutrient solution tank next time, thereby reducing the effect of microbial agent cultivation, and it is more troublesome to take out the microbial agent cultivated in the incubator, thereby resulting in low efficiency of microbial agent cultivation. Summary of the Invention

[0005] The purpose of the present application is to solve the problem in the prior art that although the installation plate can be driven downward by the linear driving mechanism, the incubator, the microbial agent, the liquid storage tank and the stirring fan blade push plate are also driven downward, the stirring fan blade can fully stir the nutrient solution in the nutrient solution tank while moving downward, and the large amount of elements accumulated at the bottom of the nutrient solution tank can be evenly distributed in the nutrient solution, but the nutrient solution tank is internally provided with a linear driving mechanism, a stirring liquid adding mechanism and a first driving mechanism, the nutrient solution added in the nutrient solution tank can contact the multiple components of the linear driving mechanism, the stirring liquid adding mechanism and the first driving mechanism, so that the cleaning of the nutrient solution tank is more troublesome, and there can be a situation that the cleaning is not in place, so that the nutrient solution can be easily contaminated when the nutrient solution is added into the nutrient solution tank next time, thereby reducing the effect of microbial agent cultivation, and the microbial agent cultivated in the incubator is more troublesome to take out, thereby reducing the efficiency of microbial agent cultivation.

[0006] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme: a culture device for agricultural microbial agent production, comprising: an incubator, a box cover is detachably mounted on the top of the incubator, a plurality of liquid discharge pipes are fixedly connected to the bottom of the inner wall of the box cover, a one-way valve is fixedly connected to the inner wall near the top of the liquid discharge pipe, a plurality of air inlet pipes are fixedly connected to the bottom of the inner wall of the incubator, a high molecular microporous membrane is fixedly connected to the inner wall near the top of the air inlet pipe, a connecting frame is fixedly connected to the bottom of the incubator, a partition is fixedly connected to the inner wall of the connecting frame, the liquid discharge pipe is fixedly connected to the partition, a gas pump is fixedly connected to one side of the incubator, an L-shaped pipe one is fixedly connected to the gas pump, an L-shaped pipe two is fixedly connected to the outer surface of the L-shaped pipe one near the gas pump, the L-shaped pipe two is fixedly connected to the connecting frame, an electromagnetic valve one is fixedly connected to the inner wall of the L-shaped pipe two near the L-shaped pipe one, a plurality of shunt pipes are equidistantly fixedly connected to one side of the connecting frame, the plurality of shunt pipes are fixedly connected to the L-shaped pipe one, a plurality of air outlet pipes are equidistantly fixedly connected to the outer surface of the shunt pipe, an electromagnetic valve two is fixedly connected to the inner wall of the L-shaped pipe one near the bend, a rotating blade is rotatably connected to the outer surface of the liquid discharge pipe, two connecting rods are symmetrically fixedly connected to the bottom of the rotating blade, a stirring blade is fixedly connected to one end of the two connecting rods, a plurality of through holes are formed in the stirring blade, a plurality of through holes are formed in the stirring blade, and a plurality of culture dishes are arranged below the incubator.

[0007] Preferably, one end of the L-shaped pipe two extends to the inside of the connecting frame and is located above the partition, the plurality of shunt pipes are located below the partition, the L-shaped pipe one, the plurality of shunt pipes and the plurality of air outlet pipes are connected in communication, and the L-shaped pipe one and the L-shaped pipe two are connected in communication.

[0008] Preferably, the inner wall of the bottom of the box cover is sealingly and fixedly connected with a transparent elastic film, the inner part of the top of the incubator is fixedly connected with an air pressure sensor, and the top of the box cover is fixedly connected with a transparent tempered glass.

[0009] Preferably, the incubator, the plurality of air inlet pipes and the upper space of the connecting frame are connected, and the inner wall of the air inlet pipe close to the microporous membrane is fixedly connected with non-woven fabric.

[0010] Preferably, the bottom of the connecting frame is fixedly connected with a bottom frame, the opposite sides of the bottom frame are both provided with two guide grooves, the inside of the guide grooves is slidingly connected with a round rod, one end of the plurality of round rods is fixedly connected with a placing plate, the top of the placing plate is provided with a plurality of round holes, and the outer surface of the culture dish is movably connected in the inside of the round holes.

[0011] Preferably, the opposite sides of the inner wall of the bottom frame are rotatably connected with a lead screw, the opposite sides of the inner wall of the bottom frame are fixedly connected with a guide rod, the outer surface of the lead screw is threadedly connected with a movable plate, the movable plate is slidingly connected with the guide rod, one side of the bottom frame is fixedly connected with a motor, and the output end of the motor is fixedly connected with one end of the lead screw.

[0012] Preferably, the top of the movable plate is symmetrically slidingly connected with two movable rods, and the two movable rods are symmetrically fixedly connected with the bottom of the placing plate.

[0013] Preferably, the outer surface of the movable rod is provided with a spring, and the spring is fixedly connected with the movable plate and the placing plate.

[0014] A use method of a culture device for the production of agricultural microbial inoculants, comprising the following steps:

[0015] S1, the controller controls the air pump to start, at this time, the electromagnetic valve one is in the open state, and the electromagnetic valve two is in the closed state, so that the gas is transported to the closed space formed between the incubator, the connecting frame and the partition through the L-shaped pipe one and the L-shaped pipe two, the gas pressure in the closed space is increased, the gas flows upward through the air inlet pipe, and then passes through the non-woven fabric and the microporous membrane, a large number of micro-bubbles are generated under the action of the microporous membrane, and are uniformly dispersed in the nutrient solution, so that the nutrient solution flows and mixes, and the nutrient solution is prevented from penetrating into the air inlet pipe through the microporous membrane, at the same time, the gas pressure in the incubator is increased, the transparent elastic film is elastically deformed, the deformation of the transparent elastic film can make the gas pressure in the incubator rise slowly, so that the nutrient solution is always in a stirring state for a period of time, when the gas pressure between the incubator and the transparent elastic film reaches a certain value, the one-way valve is opened at this time, and under the restoring force of the transparent elastic film, the nutrient solution in the incubator flows into the inside of the culture dish through the liquid discharge pipe under the action of the gas pressure;

[0016] S2, the air pressure inside the incubator is detected by the air pressure sensor, when the air pressure inside the incubator changes from rising to falling, the signal is transmitted to the controller through the air pressure sensor, then the electromagnetic valve one is closed and the electromagnetic valve two is opened through the controller, so that the gas is transported to the lower part of the baffle through the L-shaped pipe one, the shunt pipe and the air outlet pipe, and blows to the rotating blade, so that the rotating blade rotates, synchronously drives the connecting rod and the stirring piece to rotate, fully stirs and mixes the nutrient solution and the bacterial agent in the culture dish, and generates bubbles under the cooperation of the through hole;

[0017] S3, the controller controls the motor to start, so that the output shaft drives the screw rod to rotate, the movable plate moves along the outer surface of the screw rod to one side, synchronously drives the movable rod, the placing plate and the culture dish to move to one side, synchronously makes the round rod slide downward in the guide groove, when the round rod slides along the upper part of the inclined groove, the culture dish and the stirring piece can be separated without collision, then the round rod slides slowly along the lower part of the inclined groove of the guide groove, synchronously makes the movable rod slide downward, makes the spring compress, makes the placing plate and the culture dish move from the lower part of the incubator to the side, at this time the worker takes out the culture dish from the inside of the round hole, and the microbial agent is taken out;

[0018] S4, by placing the culture dish containing a small amount of bacterial agent into the round hole, the controller controls the motor to rotate reversely, so that the movable plate moves along the outer surface of the screw rod to the other side, synchronously drives the movable rod, the placing plate and the culture dish to move to one side, makes the round rod slide upward along the guide groove, under the reset force of the spring, when the round rod slides into the upper part of the inclined groove of the guide groove, the placing plate and the culture dish can move upward by a proper distance, at this time the stirring piece is just in the inside of the culture dish.

[0019] Compared with the prior art, the advantages and positive effects of the present application are that,

[0020] 1. The present application, by the controller controls the air pump to start, at this time electromagnetic valve one is in the open state, electromagnetic valve two is in the closed state, so that the gas through L-shaped pipe one and L-shaped pipe two is delivered to the incubator, the connecting frame and the partition between the closed space formed, the gas pressure in the closed space increases, the gas flows upwards through the air inlet pipe, and in turn through the non-woven fabric and the polymer microporous membrane, under the action of the polymer microporous membrane, more micro-bubbles can be generated, uniformly dispersed in the nutrient solution, the nutrient solution flows and mixes, realizes the stirring effect of the nutrient solution, and prevents the nutrient solution from penetrating into the air inlet pipe through the polymer microporous membrane, at the same time, the air pressure in the incubator is increased, the transparent elastic film is elastically deformed, the air pressure in the incubator can be slowly increased through the deformation of the transparent elastic film, the nutrient solution is always in the stirring state in a period of time, when the air pressure between the incubator and the transparent elastic film reaches a certain value, due to the opening direction of the one-way valve is from the inside of the incubator to the outside, so that the one-way valve is opened at this time, and under the restoring force of the transparent elastic film, the nutrient solution in the incubator flows into the inside of the culture dish under the action of the air pressure through the liquid discharge pipe, the inside of the culture dish has been added with bacteria agent in advance, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to mix with the bacteria agent can be completed without using more structure parts, when the incubator needs to be cleaned, only the incubator is cleaned by removing the box cover, it is relatively simple, avoids the pollution due to incomplete cleaning, thereby improving the effect of subsequent microbial agent culture.

[0021] 2. The present application, by the air pressure sensor detects the air pressure in the incubator, when the air pressure in the incubator changes from rising to falling, the signal can be transmitted to the controller through the air pressure sensor, then the controller controls electromagnetic valve one to close and electromagnetic valve two to open, so that the gas is delivered to the lower part of the partition through L-shaped pipe one, shunt pipe and air outlet pipe, blows to the rotating blade, makes the rotating blade rotate, synchronously drives the connecting rod and the stirring piece to rotate, so that the nutrient solution and bacteria agent in the culture dish can be fully stirred and mixed, and under the cooperation of the through hole, the bubbles can be generated, further improving the stirring and mixing effect of the nutrient solution and bacteria agent.

[0022] 3、The application, through the controller controls the motor start, makes its output shaft drive the screw rod to rotate, then under the action of the screw rod and the movable plate threaded connection, and under the cooperation of the guide rod to the movable plate limit guide, can make the movable plate along the outer surface of the screw rod to one side, synchronous drive movable rod, placement plate and petri dish to one side, synchronous make the round rod in the inside of the guide groove slide down, the upper part of the guide groove is set to the inclined groove with larger inclination, the lower part is set to the inclined groove with smaller inclination, when the round rod slides along the upper part inclined groove, can make the petri dish and the stirring piece separate, and no collision occurs, then make the round rod slowly slide down along the lower part inclined groove of the guide groove, synchronous make the movable rod slide down, make the spring compress, make the placement plate and petri dish etc. from the lower part of the incubator to the side, at this time, the staff can take out the petri dish from the inside of the round hole, which is convenient for the staff to take out the microorganism agent cultured in the petri dish, is relatively simple, thereby improving the efficiency of microorganism agent culture. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The side view structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0024] Figure 2 The bottom view structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0025] Figure 3 The partial cross-section side view structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0026] Figure 4 The partial cross-section bottom view structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided. Figure 3 The enlarged structure schematic diagram of A in the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0027] Figure 5 The partial cross-section bottom view structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0028] Figure 6 The enlarged structure schematic diagram of B in the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0029] Figure 7 The overall cross-section structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0030] Figure 8 The non-woven fabric cross-section structural schematic diagram of the culture device for agricultural microbial inoculant production and use method provided by the application is provided.

[0031] Legend:

[0032] 1, incubator; 101, box cover; 102, transparent tempered glass; 103, liquid inlet pipe; 104, liquid outlet pipe; 105, air inlet pipe; 106, transparent elastic film; 107, one-way valve; 108, high molecular microporous membrane; 109, non-woven fabric; 2, connecting frame; 201, shunt pipe; 202, L-shaped pipe one; 203, L-shaped pipe two; 204, air pump; 205, partition plate; 206, air outlet pipe; 207, rotating blade; 208, connecting rod; 209, stirring blade; 3, bottom frame; 301, guide groove; 3011, round rod; 302, screw rod; 303, motor; 304, guide rod; 305, movable plate; 306, movable rod; 307, spring; 308, placing plate; 309, culture dish; 310, round hole. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0034] Embodiments, such as Figure 1 - Figure 8As shown, the present application provides a technical scheme: a culture device for agricultural microbial inoculant production, comprising: a culture box 1, a box cover 101 is detachably installed on the top of the culture box 1, a plurality of liquid discharge pipes 104 are fixedly connected to the bottom of the inner wall of the box cover 101, a one-way valve 107 is fixedly connected to the inner wall near the top of the liquid discharge pipe 104, a plurality of air inlet pipes 105 are fixedly connected to the bottom of the inner wall of the culture box 1, a high molecular microporous membrane 108 is fixedly connected to the inner wall near the top of the air inlet pipe 105, a connecting frame 2 is fixedly connected to the bottom of the culture box 1, a partition plate 205 is fixedly connected to the inner wall of the connecting frame 2, the liquid discharge pipe 104 is fixedly connected to the partition plate 205, a gas pump 204 is fixedly connected to one side of the culture box 1, an L-shaped pipe one 202 is fixedly connected to the gas outlet end of the gas pump 204, an L-shaped pipe two 203 is fixedly connected to the outer surface of the L-shaped pipe one 202 near the gas pump 204, the L-shaped pipe two 203 is fixedly connected to the connecting frame 2, an electromagnetic valve one is fixedly connected to the inner wall of the L-shaped pipe two 203 near the L-shaped pipe one 202, a plurality of shunt pipes 201 are equidistantly fixedly connected to one side of the connecting frame 2, the plurality of shunt pipes 201 are fixedly connected to the L-shaped pipe one 202, a plurality of gas outlet pipes 206 are equidistantly fixedly connected to the outer surface of the shunt pipe 201, an electromagnetic valve two is fixedly connected to the inner wall of the L-shaped pipe one 202 near the bend, a rotating blade 207 is rotatably connected to the outer surface of the liquid discharge pipe 104, two connecting rods 208 are fixedly connected to the bottom of the rotating blade 207 in a symmetrical manner, a stirring blade 209 is fixedly connected to one end of the two connecting rods 208, a plurality of through holes are formed in the stirring blade 209, a plurality of culture dishes 309 are arranged below the culture box 1.

[0035] Further, as shown in the drawings, Figure 1 - Figure 8 As shown, one end of the L-shaped pipe two 203 extends to the inside of the connecting frame 2, above the partition plate 205, the plurality of shunt pipes 201 are all below the partition plate 205, the L-shaped pipe one 202, the plurality of shunt pipes 201 and the plurality of gas outlet pipes 206 are connected, the L-shaped pipe one 202 and the L-shaped pipe two 203 are connected, through the above arrangement, a closed space is formed between the culture box 1, the connecting frame 2 and the partition plate 205, so that the gas can pass through the L-shaped pipe one 202, the shunt pipe 201 and the gas outlet pipe 206 in turn, and blow to the rotating blade 207, so that the gas can pass through the L-shaped pipe two 203 into the closed space.

[0036] Further, as shown in the drawings, Figure 1 - Figure 8As shown, the inner wall of the bottom of the box cover 101 is sealingly and fixedly connected with a transparent elastic film 106, the inside of the top of the incubator 1 is fixedly connected with an air pressure sensor, the top of the box cover 101 is fixedly connected with a transparent tempered glass 102, through the above setting, when the air pressure inside the incubator 1 is enhanced, the transparent elastic film 106 is elastically deformed, through the deformation of the transparent elastic film 106, the air pressure inside the incubator 1 can be slowly increased, so that the nutrient solution is always in an agitated state for a period of time, through the setting of the air pressure sensor, the air pressure inside the incubator 1 is convenient to detect, through the setting of the transparent tempered glass 102, the nutrient solution inside the incubator 1 is convenient to observe.

[0037] Further, as shown in Figure 1 - Figure 8 As shown, the incubator 1, the plurality of air inlet pipes 105 and the upper space of the connecting frame 2 are connected, the inner wall of the air inlet pipe 105 close to the high molecular microporous membrane 108 is fixedly connected with a non-woven fabric 109, through the above setting, the gas in the connecting frame 2 can enter the incubator 1 through the air inlet pipe 105, through the setting of the non-woven fabric 109, a certain gas permeability effect is achieved, and the high molecular microporous membrane 108 is supported.

[0038] Further, as shown in Figure 1 - Figure 8 As shown, the bottom of the connecting frame 2 is fixedly connected with a bottom frame 3, the opposite sides of the bottom frame 3 are both provided with two guide grooves 301, the inside of the guide groove 301 is slidingly connected with a round rod 3011, one end of the plurality of round rods 3011 is fixedly connected with a placing plate 308, the top of the placing plate 308 is provided with a plurality of round holes 310, the outer surface of the culture dish 309 is movably connected in the inside of the round hole 310, when the movable rod 306, the placing plate 308 and the culture dish 309 move to one side, the round rod 3011 in the guide groove 301 is synchronously and downwardly slid, when the round rod 3011 slides along the upper inclined groove, the culture dish 309 and the stirring blade 209 can be separated without collision, then the round rod 3011 slowly slides downwardly along the lower inclined groove of the guide groove 301, so that the placing plate 308 and the culture dish 309 move from the bottom of the incubator 1 to the side.

[0039] Further, as shown in Figure 1 - Figure 8As shown in the drawings, the opposite side of the inner wall of the bottom frame 3 is rotatably connected with a lead screw 302, the opposite side of the inner wall of the bottom frame 3 is fixedly connected with a guide rod 304, the outer surface of the lead screw 302 is threadedly connected with a movable plate 305, the movable plate 305 is slidably connected with the guide rod 304, one side of the bottom frame 3 is fixedly connected with a motor 303, the output end of the motor 303 is fixedly connected with one end of the lead screw 302, the motor 303 can be started by the controller to drive the output shaft to rotate the lead screw 302, then under the cooperation of the thread connection between the lead screw 302 and the movable plate 305 and the limiting and guiding of the guide rod 304 to the movable plate 305, the movable plate 305 can move along the outer surface of the lead screw 302 to one side.

[0040] Further, as shown in the drawings, Figure 1 - Figure 8 As shown in the drawings, the top of the movable plate 305 is symmetrically slidably connected with two movable rods 306, and the two movable rods 306 are symmetrically fixedly connected with the bottom of the placement plate 308. Through the above setting, the movable rod 306 and the placement plate 308 can slide up and down by an appropriate distance.

[0041] Further, as shown in the drawings, Figure 1 - Figure 8 As shown in the drawings, the outer surface of the movable rod 306 is provided with a spring 307, and the spring 307 is fixedly connected with the movable plate 305 and the placement plate 308, respectively. Under the reset force of the spring 307, the placement plate 308 has an upward movement force.

[0042] Working principle: in use, through the liquid inlet pipe 103 to the inside of the incubator 1 add appropriate amount of nutrient solution, and the liquid inlet pipe 103 sealing, again through the controller control air pump 204 start, at this moment electromagnetic valve one is in the open state, electromagnetic valve two is in the closed state, so that the gas through the L-shaped pipe one 202 and L-shaped pipe two 203 is delivered to the closed space between the incubator 1, connecting frame 2 and the partition 205, make the gas pressure in the closed space increases, make the gas flow upwards through the air inlet pipe 105, and in turn through the non-woven fabric 109 and the high molecular microporous membrane 108, through the action of the high molecular microporous membrane 108, can make more micro bubbles, uniform dispersion in the nutrient solution, make the nutrient solution flow and mixing, realize the stirring effect of the nutrient solution, and prevent the nutrient solution from penetrating into the air inlet pipe 105 through the high molecular microporous membrane 108, at the same time, the gas pressure in the incubator 1 is enhanced, the transparent elastic film 106 is deformed, through the deformation of the transparent elastic film 106, the gas pressure in the incubator 1 rises slowly, so that the nutrient solution is always in the stirring state in a period of time, when the gas pressure between the incubator 1 and the transparent elastic film 106 reaches a certain value, because the opening direction of the one-way valve 107 is from the inside of the incubator 1 to the outside, so that the one-way valve 107 opens at this moment, and under the reset force of the transparent elastic film 106, the nutrient solution in the incubator 1 flows into the inside of the culture dish 309 under the action of gas pressure through the liquid outlet pipe 104, the inside of the culture dish 309 has been added with bacteria agent in advance, so that without using more structure parts, the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish 309 to mix with the bacteria agent can be completed, when the incubator 1 needs to be cleaned, only the box cover 101 is disassembled, the residual nutrient solution in the incubator 1 is cleaned, which is relatively simple, avoids the pollution due to incomplete cleaning, thereby improving the effect of subsequent microbial agent culture, the gas pressure in the incubator 1 is detected by the gas pressure sensor, when the gas pressure in the incubator 1 changes from rising to falling, the signal can be transmitted to the controller through the gas pressure sensor, and then the controller controls the electromagnetic valve one to close and the electromagnetic valve two to open, so that the gas is delivered to the lower side of the partition 205 through the L-shaped pipe one 202, the shunt pipe 201 and the air outlet pipe 206, and blows towards the rotating blade 207, so that the rotating blade 207 rotates, synchronously drives the connecting rod 208 and the stirring piece 209 to rotate, so that the nutrient solution and the bacteria agent in the culture dish 309 can be fully stirred and mixed, and the bubbles can be generated under the cooperation of the through hole, further improving the stirring and mixing effect of the nutrient solution and the bacteria agent, when the nutrient solution and the bacteria agent are stirred and mixed, and the culture is completed, the motor 303 can be started at this moment through the controller, so that the output shaft drives the lead screw 302 to rotate, then under the action of the screw thread connection between the lead screw 302 and the movable plate 305, and under the cooperation of the guide rod 304 limiting and guiding the movable plate 305, the movable plate 305 can move along the outer surface of the lead screw 302 to one side,Synchronous belt activity pole 306, 308 and placing plate culture dish 309 etc. to one side, synchronous make round pole 3011 in guide groove 301 inside down slide, guide groove 301 upper part sets into the inclined groove of greater gradient, lower part sets into the inclined groove of smaller gradient, when round pole 3011 along upper part inclined groove slide, can make culture dish 309 and stirring blade 209 disengage, will not collide, then make round pole 3011 along guide groove 301 lower part inclined groove slowly slide down, synchronous make activity pole 306 slide down, make spring 307 compress, make placing plate 308 and culture dish 309 etc. from the lower part of incubator 1 move to the side, at this time staff can take out culture dish 309 from the inside of round hole 310, this way is convenient for staff to take out the microorganism culture agent cultivated in culture dish 309, relatively simple, thereby improve the efficiency of microorganism culture agent cultivation, when need to cultivate microorganism culture agent again, can place culture dish 309 with a small amount of culture agent in round hole 310, then control motor 303 reverse rotation through controller, make movable plate 305 along the outer surface of screw 302 move to the other side, synchronous belt activity pole 306, 308 and placing plate 309 etc. to one side, make round pole 3011 along guide groove 301 slide up, through the reset action force of spring 307, can make placing plate 308 have an upward movement force, when round pole 3011 slides into guide groove 301 upper part inclined groove, can make placing plate 308 and culture dish 309 move up a proper distance, at this time stirring blade 209 is just in the inside of culture dish 309.

[0043] The above description is only the preferred embodiment of the present application, and is not intended to limit the present application to other forms, any skilled in the art can use the above disclosed technical content to change or modify equivalent embodiments applied to other fields, but any simple modification, equivalent change and modification of the above embodiments without departing from the technical solution content of the present application, according to the technical essence of the present application, still belongs to the protection scope of the technical solution of the present application.

Claims

1. A culture device for producing agricultural microbial agents, characterized in that: include: The incubator (1) is provided with a detachable sealed cover (101) on the top of the incubator (1), a plurality of drainage pipes (104) are fixedly connected to the bottom of the inner wall of the cover (101), a one-way valve (107) is fixedly connected to the inner wall of the drainage pipe (104) near the top, a plurality of air inlet pipes (105) are fixedly connected to the bottom of the inner wall of the incubator (1), a polymer microporous membrane (108) is fixedly connected to the inner wall of the air inlet pipe (105) near the top, and the incubator (1) is provided with a plurality of air inlet pipes (105) fixedly connected to the inner wall of the air inlet pipe (105) near the top. The bottom of the incubator (1) is fixedly connected to a connecting frame (2), the inner wall of the connecting frame (2) is fixedly connected to a partition (205), the drainage pipe (104) is fixedly connected to the partition (205), one side of the incubator (1) is fixedly connected to an air pump (204), the air outlet end of the air pump (204) is fixedly connected to an L-shaped tube 1 (202), the outer surface of the L-shaped tube 1 (202) close to the air pump (204) is fixedly connected to an L-shaped tube 2 (203), the L-shaped tube The second tube (203) is fixedly connected to the connection frame (2); the inner wall of the L-shaped tube (203) near the first L-shaped tube (202) is fixedly connected to the first solenoid valve; one side of the connection frame (2) is fixedly connected to a plurality of shunt tubes (201) at equal intervals; the plurality of shunt tubes (201) are fixedly connected to the first L-shaped tube (202); the outer surface of the shunt tube (201) is fixedly connected to a plurality of air outlet pipes (206) at equal intervals; the inner wall of the first L-shaped tube (202) near the bend is fixedly connected to the first L-shaped tube (202); A second solenoid valve is fixedly connected to the wall, the outer surface of the discharge pipe (104) is rotatably connected to a rotating blade (207), the bottom of the rotating blade (207) is symmetrically fixedly connected to two connecting rods (208), one end of the two connecting rods (208) is fixedly connected to a stirring piece (209), the stirring piece (209) is provided with a plurality of through holes, and the stirring piece (209) is provided with a plurality of through holes. A plurality of culture dishes (309) are arranged below the incubator (1).

2. A cultivation device for producing agricultural microbial agents according to claim 1, characterized in that: One end of the second L-shaped tube (203) extends to the interior of the connection frame (2) and is located above the partition (205); the plurality of diversion tubes (201) are all located below the partition (205); the first L-shaped tube (202), the plurality of diversion tubes (201) and the plurality of air outlet tubes (206) are connected; and the first L-shaped tube (202) and the second L-shaped tube (203) are connected.

3. The cultivation device for producing agricultural microbial agents according to claim 1, characterized in that: The inner wall of the box cover (101) near the bottom is sealed and fixedly connected with a transparent elastic membrane (106), the interior of the incubator (1) near the top is fixedly connected with an air pressure sensor, and the top of the box cover (101) is fixedly connected with a transparent tempered glass (102).

4. A cultivation device for producing agricultural microbial agents according to claim 3, characterized in that: The incubator (1), the plurality of air inlet pipes (105) and the upper space of the connection frame (2) are connected to each other, and the air inlet pipes (105) are fixedly connected to the inner wall of the polymer microporous membrane (108) with a non-woven fabric (109).

5. The cultivation device for producing agricultural microbial agents according to claim 4, characterized in that: The bottom of the connecting frame (2) is fixedly connected to a bottom frame (3), and two guide grooves (301) are provided on opposite sides of the bottom frame (3). Round rods (3011) are slidably connected inside the guide grooves (301), and one end of a plurality of round rods (3011) is fixedly connected to a placement plate (308). The top of the placement plate (308) is provided with a plurality of round holes (310), and the outer surface of the culture dish (309) is movably connected inside the round holes (310).

6. The cultivation device for producing agricultural microbial agents according to claim 5, characterized in that: A screw rod (302) is rotatably connected to the opposite side of the inner wall of the bottom frame (3), a guide rod (304) is fixedly connected to the opposite side of the inner wall of the bottom frame (3), a movable plate (305) is threadedly connected to the outer surface of the screw rod (302), the movable plate (305) is slidably connected to the guide rod (304), a motor (303) is fixedly connected to one side of the bottom frame (3), and an output end of the motor (303) is fixedly connected to one end of the screw rod (302).

7. The cultivation device for producing agricultural microbial agents according to claim 6, characterized in that: The top of the movable plate (305) is symmetrically and slidingly connected to two movable rods (306), and the two movable rods (306) are symmetrically and fixedly connected to the bottom of the placement plate (308).

8. The cultivation device for producing agricultural microbial agents according to claim 7, characterized in that: The outer surface of the movable rod (306) is provided with a spring (307), and the spring (307) is fixedly connected to the movable plate (305) and the placement plate (308) respectively.

9. A method for using a cultivation device for producing agricultural microbial agents according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. The air pump (204) is started by the controller. At this time, the electromagnetic valve 1 is in the open state and the electromagnetic valve 2 is in the closed state, so that the gas is transported through the L-shaped tube 1 (202) and the L-shaped tube 2 (203) to the closed space formed between the incubator (1), the connecting frame (2) and the partition (205), so that the air pressure in the closed space is increased, and the gas flows upward through the air inlet pipe (105), and passes through the non-woven fabric (109) and the polymer microporous membrane (108) in turn. Under the action of the polymer microporous membrane (108), more tiny bubbles are generated and evenly dispersed in the nutrient solution, so that the nutrient solution flows and mixes, and the nutrient solution is prevented from passing through the high molecular weight microporous membrane (108). The molecular microporous membrane (108) enters the air inlet pipe (105), and at the same time, the air pressure inside the incubator (1) is increased, causing the transparent elastic membrane (106) to undergo elastic deformation. Through the deformation of the transparent elastic membrane (106), the air pressure inside the incubator (1) can be increased slowly, so that the nutrient solution is always in a stirring state for a period of time. When the air pressure between the incubator (1) and the transparent elastic membrane (106) reaches a certain value, the one-way valve (107) is opened, and under the reset force of the transparent elastic membrane (106), the nutrient solution inside the incubator (1) flows into the interior of the culture dish (309) through the drainage pipe (104) under the action of the air pressure. S2. The air pressure inside the incubator (1) is detected by the air pressure sensor. When the air pressure inside the incubator (1) changes from an ascending state to a descending state, the air pressure sensor transmits a signal to the controller, which then controls the electromagnetic valve 1 to close and the electromagnetic valve 2 to open, so that the gas is transported to the bottom of the partition (205) through the L-shaped tube 1 (202), the diverter tube (201) and the air outlet pipe (206), and blows air toward the rotating blade (207), so that the rotating blade (207) rotates, and synchronously drives the connecting rod (208) and the stirring blade (209) to rotate, so that the nutrient solution and the bacterial agent inside the culture dish (309) are fully stirred and mixed, and bubbles are generated with the cooperation of the through hole; S3, the controller controls the motor (303) to start, so that its output shaft drives the screw (302) to rotate, so that the movable plate (305) moves to one side along the outer surface of the screw (302), and synchronously drives the movable rod (306), the placement plate (308) and the culture dish (309) to move to one side, and synchronously causes the round rod (3011) to slide downward inside the guide groove (301). When the round rod (3011) slides along the upper inclined groove, the culture dish (309) and the stirring piece (209) can be separated without collision. Then, the round rod (3011) is slowly slid downward along the lower inclined groove of the guide groove (301), and the movable rod (306) is synchronously slid downward, so that the spring (307) is compressed, so that the placement plate (308) and the culture dish (309) move from the bottom of the incubator (1) to the side. At this time, the staff takes out the culture dish (309) from the inside of the circular hole (310), and the microbial inoculum is taken out. S4. A culture dish (309) containing a small amount of bacterial agent is placed in the circular hole (310), and the motor (303) is controlled by the controller to rotate in the opposite direction, so that the movable plate (305) moves along the outer surface of the screw rod (302) to the other side, and the movable rod (306), the placement plate (308) and the culture dish (309) are simultaneously driven to move to one side, so that the round rod (3011) slides upward along the guide groove (301). Under the reset force of the spring (307), when the round rod (3011) slides into the upper inclined groove of the guide groove (301), the placement plate (308) and the culture dish (309) can be moved upward by an appropriate distance. At this time, the stirring piece (209) is just inside the culture dish (309).

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