An apparatus for extracting dental pulp stem cells and a method for extracting the same

Abstract

The present invention discloses a dental pulp stem cell extraction device and an extraction method thereof, which relates to the technical field of dental pulp stem cell extraction. The device includes a base plate, a tooth clamping device arranged on the base plate, and a switching tooth cutting mechanism located above the tooth clamping device. The switching tooth cutting mechanism includes a switching turntable located above the rotating clamping component, a grinding component, a flushing component, a grooving component and a clamping and separating device annularly arranged at the bottom of the switching turntable. The clamping and separating device is used to clamp the groove machined on the tooth by the grooving component and separate the tooth into two halves driven by the rotating clamping component. When the dental pulp stem cell extraction device and the extraction method in the present invention are used for dental pulp stem cell extraction, the tooth can be separated to fully expose the dental pulp cavity. At the same time, the tooth can be fully cleaned during the tooth separation process to prevent the dental pulp from being contaminated, and the generation of debris during tooth separation can be avoided, thus preventing the dental pulp from being contaminated.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of dental pulp stem cell extraction, and specifically provides a dental pulp stem cell extraction device and an extraction method thereof. Background Technique

[0002] Dental pulp stem cells are a type of mesenchymal stem cells present in the dental pulp tissue inside the teeth. When extracting dental pulp stem cells, it is necessary to damage the teeth to expose the pulp cavity, strip the dental pulp from the pulp cavity, and process the dental pulp through enzymatic hydrolysis to obtain dental pulp stem cells.

[0003] The existing dental pulp extraction devices in the prior art include a dental pulp extraction tooling, a dental pulp stem cell extractor, a dental pulp transfer window, a dental pulp stem cell extraction tube, a dental pulp stem cell extraction tube carrier, a tooth body carrier, a motor, a high-efficiency filter, a tooth body clamp screw, a tooth body universal control component, a tooth body clamp, a tooth body clamp pliers, a forward X-ray machine, a lateral X-ray machine, an extraction device main body, a compressor cooling fan, and a compressor. The prior art adopts a composite rotary clamping structure, integrating functions of cutting, clamping, fixing, and separating, suitable for the extraction needs of single-root and multi-root teeth, capable of protecting the dental pulp from being damaged during extraction, and the internal tooth saw blade can be replaced.

[0004] The existing technology can protect the dental pulp from being damaged during extraction, but it is not convenient for fully cutting and exposing the pulp cavity and for residue-free extraction of the dental pulp tissue. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to provide a dental pulp stem cell extraction device and an extraction method thereof to solve the technical problems raised in the above background technique.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A dental pulp stem cell extraction device includes a base plate, a tooth clamping device provided on the base plate, and a switching type tooth cutting mechanism located above the tooth clamping device. The tooth clamping device includes a lifter provided on the base plate, a lifting plate provided at the execution end of the lifter, a planar mover provided on the top of the lifting plate, and a rotary clamping component provided at the execution end of the planar mover;

[0007] The switching type tooth cutting mechanism includes a switching turntable located above the rotary clamping component, a grinding component, a flushing component, a grooving component, and a clamping and separating device annularly arrayed at the bottom of the switching turntable. The clamping and separating device is used to clamp the groove machined on the tooth by the grooving component and separate the tooth into two halves under the drive of the rotary clamping component;

[0008] The clamping and separating device includes a track provided at the bottom of the switching turntable, two second sliders that are slidably connected to the track and symmetrically arranged, two first springs symmetrically provided at both ends of the inner wall of the track, and an automatic card slot component at one end of the bottom of the second slider and a magnetic linkage component at the other end.

[0009] Preferably, the rotating clamping component includes a substrate provided at the execution end of the planar mover, two first sliders that are slidably connected to the top of the substrate and symmetrically arranged, a first motor provided on the top of the substrate, a bidirectional lead screw provided at the execution end of the first motor and threadedly connected to the first slider, a rotating rod rotatably connected to the top of the side wall of the first slider, a suction cup provided at the end of the rotating rod, and a second motor provided on one of the first sliders and used to drive the rotating rod to rotate. In this preferred embodiment, the stable clamping and rotation of the teeth are achieved through the rotating clamping component.

[0010] Preferably, the grinding component includes an n-shaped frame provided at the bottom of the switching turntable, a grinding roller rotatably connected to the n-shaped frame and horizontally arranged, and a driving motor provided on the outer wall of the n-shaped frame and used to drive the grinding roller to rotate. In this preferred embodiment, the grinding of the tooth surface is achieved through the grinding component, which facilitates the cleaning of the tooth surface and can flatten the tooth surface at the same time to reduce the possibility of waste chips generated during tooth separation.

[0011] Preferably, the flushing component includes a driving cylinder provided on the top of the switching turntable and the execution end of which penetrates the switching turntable, a finger cylinder provided at the execution end of the driving cylinder, two sealing boxes symmetrically provided at the execution end of the finger cylinder, a water inlet pipe with one end communicating with one of the sealing boxes, and a drain pipe with one end communicating with the other sealing box. In this preferred embodiment, the flushing component facilitates the cleaning of the tooth surface.

[0012] Preferably, the grooving component includes a power motor provided on the top of the switching turntable and the execution end of which penetrates the switching turntable, an extension rod provided at the execution end of the power motor, and a grooving ball head provided at the end of the extension rod. In this preferred embodiment, the grooving of the tooth surface is facilitated through the grooving component to prepare for the tooth separation work in advance.

[0013] Preferably, the automatic card slot component includes a cross bar provided on the outer wall of the second slider, a first clamping block provided at the bottom of the cross bar, vertical bars symmetrically provided at both ends of the cross bar and hinged to the cross bar, a second clamping block provided on the side wall of the vertical bar, a magnetic block provided on the side of the vertical bar away from the second clamping block, an n-shaped bar with its bottom connected to the top of the cross bar, and two first electromagnetic blocks symmetrically provided on both sides of the n-shaped bar and abutting against the magnetic block. In this preferred embodiment, the automatic card slot component facilitates being inserted into the annular groove formed on the tooth surface, facilitating the separation of teeth of different sizes.

[0014] Preferably, the magnetic attraction linkage component includes a positioning box provided on the outer wall of the second slider, a second electromagnetic block with its top slidably connected to the inner wall of the positioning box, a second spring provided at the top of the inner wall of the positioning box, two extension plates symmetrically provided on both sides of the positioning box, an arc-shaped slide rail provided at the end of the extension plate, and a connecting rod with one end connected to the outer wall of the vertical bar and the other end slidably connected to the arc-shaped slide rail. In this preferred embodiment, the magnetic attraction linkage component facilitates magnetic attraction connection or separation with the execution end of the tooth clamping device.

[0015] Preferably, the elevator includes a plurality of guide sleeves provided on the base plate, guide posts with their bottoms slidably connected to the inner walls of the guide sleeves and their tops connected to the bottom of the lifting plate, a positioning sleeve provided at the center of the top of the base plate, a positioning rod with its bottom slidably connected to the inner wall of the positioning sleeve and its top connected to the bottom of the lifting plate, a driving groove embedded at the bottom of the positioning rod, a first stepping motor provided at the bottom of the base plate, and a driving lead screw provided at the execution end of the first stepping motor and threadedly connected to the driving groove;

[0016] The planar mover includes a first linear module provided on the top of the lifting plate, and a second linear module provided at the execution end of the first linear module and distributed perpendicular to the first linear module, and the execution end of the second linear module is connected to the bottom of the substrate. In this preferred embodiment, the elevator realizes the stable lifting of the tooth to be processed, and the planar mover realizes the stable movement of the tooth to be processed on the plane.

[0017] Preferably, a second stepping motor is provided on the base plate, the execution end of the second stepping motor is connected to one end of a driving shaft, and the other end of the driving shaft penetrates through the lifting plate and is connected to the bottom of the switching turntable. In this preferred embodiment, the second stepping motor realizes the stable rotation of the switching turntable, so as to move the grinding component, the flushing component, the grooving component, and the clamping and separating device to the working positions.

[0018] According to the above technical solution of a dental pulp stem cell extraction device, an extraction method of a dental pulp stem cell extraction device will also be provided, including the following steps:

[0019] Step 1: X-ray imaging of teeth; perform X-ray imaging on teeth to obtain information about the dental pulp cavity of the teeth;

[0020] Step 2: Grinding treatment of teeth; the tooth clamping device clamps the teeth, and after clamping, the grinding component grinds the outer surface of the teeth to obtain Tooth 1;

[0021] Step 3: Grooving treatment of teeth; the grooving component performs circular grooving on the surface of Tooth 1 according to the dental pulp cavity information to obtain Tooth 2;

[0022] Step 4: Cleaning of teeth; the rinsing component rinses the surface of Tooth 2 to obtain Tooth 3;

[0023] Step 5: Separation of teeth; the tooth clamping device applies a squeezing force to Tooth 3 and maintains it for a unit time. After completion, the clamping and separating device clamps the circular groove on Tooth 3 and applies a separating force to the tooth through the tooth clamping device and maintains it for a unit time to complete one tooth separation process. Repeat the tooth separation process until Tooth 3 is separated into two halves to obtain two Tooth 4s;

[0024] Step 6: Extraction of dental pulp tissue; use tweezers to peel the dental pulp tissue from the two Tooth 4s and place it in sterile PBS;

[0025] Step 7: Digestion of dental pulp tissue; cut the dental pulp tissue into pieces and mix it with preheated digestive enzyme, and perform water bath digestion for a unit time to obtain Digestive Solution 1;

[0026] Step 8: Preparation of cell suspension; add an equal volume of complete medium to Digestive Solution 1, and after mixing evenly, perform centrifugation. After centrifugation, remove the supernatant, and resuspend the cells with dental pulp mesenchymal stem cell complete medium;

[0027] Step 9: Inoculation and culture; inoculate the resuspended cells into a culture dish and place it in an incubator for culture to complete the extraction of dental pulp stem cells.

[0028] In summary, the present invention mainly has the following beneficial effects:

[0029] When the dental pulp stem cell extraction device and extraction method in the present invention are used for extracting dental pulp stem cells, they can separate the teeth to fully expose the dental pulp cavity. At the same time, during the tooth separation process, the teeth can be fully cleaned to prevent the dental pulp from being contaminated, and it can avoid the generation of debris during tooth separation and prevent the dental pulp from being contaminated;

[0030] The device in the present invention realizes the stable fixation and position adjustment of teeth through a tooth clamping device to facilitate the separation and processing of teeth. In the tooth clamping device, the stable lifting of the teeth to be processed is realized through a lifter, the stable movement of the teeth to be processed on a plane is realized through a planar mover, and the stable clamping and rotation of the teeth are realized through a rotating clamping component.

[0031] The stable rotation of the switching turntable is realized through a second stepping motor, so as to move the grinding component, flushing component, grooving component and clamping and separating device to the working position. The grinding of the tooth surface is realized through the grinding component, which facilitates the cleaning of the tooth surface and can flatten the tooth surface at the same time, so as to reduce the possibility of waste chips generated during tooth separation. The flushing component facilitates the cleaning of the tooth surface, and the grooving component facilitates the grooving treatment of the tooth surface, so as to make preparations in advance for the tooth separation work.

[0032] The stable separation of teeth is realized through a clamping and separating device. In the clamping and separating device, the automatic clamping slot component facilitates clamping into the annular groove opened on the tooth surface, which is convenient for separating teeth of different sizes, and the magnetic linkage component facilitates magnetic connection or separation with the execution end of the tooth clamping device. Brief Description of the Drawings

[0033] Figure 1 It is an isometric view of the overall structure of the device of the present invention;

[0034] Figure 2 It is an exploded view of the overall structure of the device of the present invention;

[0035] Figure 3 It is an exploded view of the structure of the tooth clamping device of the present invention;

[0036] Figure 4 It is an exploded view of the bottom structure of the switching turntable of the present invention;

[0037] Figure 5 It is an exploded view of the structure of the clamping and separating device of the present invention;

[0038] Figure 6 It is an exploded view of the structure of the magnetic linkage component of the present invention;

[0039] Figure 7 It is a sectional view of the overall structure of the device of the present invention;

[0040] Figure 8 It is a sectional view of the structure of the switching turntable of the present invention;

[0041] Figure 9 It is a flowchart of the extraction method of the present invention.

[0042] Description of the drawings: 10. Base plate; 20. Tooth clamping device; 21. Lifter; 211. Guide sleeve; 212. Guide post; 213. Positioning sleeve; 214. Positioning rod; 215. Driving groove; 216. First stepping motor; 217. Driving lead screw; 22. Lifting plate; 23. Planar mover; 231. First linear module; 232. Second linear module; 24. Rotating clamping member; 241. Substrate; 242. First slider; 243. First motor; 244. Bi-directional lead screw; 245. Rotating rod; 246. Suction cup; 247. Second motor; 30. Switching turntable; 301. Second stepping motor; 302. Driving shaft; 31. Grinding member; 311. N-shaped frame; 312. Grinding roller; 313. Driving motor; 32. Flushing member; 321. Driving cylinder; 322. Finger cylinder; 323. Sealing box; 324. Water inlet pipe; 325. Drain pipe; 33. Grooving member; 331. Power motor; 332. Extension rod; 333. Grooving ball head; 40. Clamping and separating device; 41. Track; 42. Second slider; 43. First spring; 44. Automatic clamping slot member; 441. Cross bar; 4411. First clamping block; 442. Vertical bar; 4421. Second clamping block; 443. Magnetic block; 444. N-shaped rod; 445. First electromagnet; 45. Magnetic linkage member; 451. Positioning box; 452. Second electromagnet; 453. Second spring; 454. Extension plate; 455. Arc-shaped slide rail; 456. Connecting rod. Detailed implementation mode

[0043] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present invention and should not be construed as a limitation to the present invention.

[0044] The following will describe the embodiments according to the overall structure of the present invention. Embodiment

[0045] Please refer specifically to the attached Figure 1 、 2As shown in FIGS. 3, 7, and 9, in a preferred embodiment of the present invention, a dental pulp stem cell extraction device includes a base plate 10, a tooth clamping device 20 provided on the base plate 10, and a switching tooth cutting mechanism located above the tooth clamping device 20. The tooth clamping device 20 includes a lifter 21 provided on the base plate 10, a lifting plate 22 provided at the execution end of the lifter 21, a planar mover 23 provided at the top of the lifting plate 22, and a rotating clamping member 24 provided at the execution end of the planar mover 23. The rotating clamping member 24 includes a base plate 241 provided at the execution end of the planar mover 23, two first sliders 242 that are symmetrically arranged and slidably connected to the top of the base plate 241, a first motor 243 provided at the top of the base plate 241, a bidirectional lead screw 244 provided at the execution end of the first motor 243 and threadedly connected to the first sliders 242, a rotating rod 245 rotatably connected to the top of the side wall of the first slider 242, a suction cup 246 provided at the end of the rotating rod 245, and a second motor 247 provided on one of the first sliders 242 and used to drive the rotating rod 245 to rotate. The lifter 21 includes a plurality of guide sleeves 211 provided on the base plate 10, a guide post 212 whose bottom is slidably connected to the inner wall of the guide sleeve 211 and whose top is connected to the bottom of the lifting plate 22, a positioning sleeve 213 provided at the center of the top of the base plate 10, a positioning rod 214 whose bottom is slidably connected to the inner wall of the positioning sleeve 213 and whose top is connected to the bottom of the lifting plate 22, a driving groove 215 embedded at the bottom of the positioning rod 214, a first stepping motor 216 provided at the bottom of the base plate 10, and a driving lead screw 217 provided at the execution end of the first stepping motor 216 and threadedly connected to the driving groove 215. The planar mover 23 includes a first linear module 231 provided at the top of the lifting plate 22, and a second linear module 232 provided at the execution end of the first linear module 231 and perpendicularly distributed to the first linear module 231. The execution end of the second linear module 232 is connected to the bottom of the base plate 241.

[0046] It should be noted that in this embodiment, when extracting dental pulp stem cells, it is necessary to first take an X-ray of the tooth. The X-ray image information after shooting is uploaded to the controller. After analysis by the controller, the dental pulp cavity information of the tooth is obtained. After obtaining the dental pulp cavity information, the tooth needs to be polished, grooved, cleaned, and separated in sequence to separate the tooth and fully expose the dental pulp cavity. After the dental pulp cavity is fully exposed, the dental pulp tissue is peeled from the middle of the two teeth with forceps and placed in sterile PBS. After the dental pulp tissue is minced, it is mixed with preheated digestive enzyme. After water bath digestion for a unit time, an equal volume of complete medium is added. After mixing evenly, centrifugation is performed. After centrifugation is completed, the supernatant is removed. The cells are resuspended with dental mesenchymal stem cell complete medium. The resuspended cells are inoculated into a culture dish and placed in an incubator for culture to complete the extraction of dental pulp stem cells;

[0047] Further, when the tooth clamping device 20 clamps the tooth, the lifter 21 can drive the tooth to move up and down, the planar mover 23 can drive the tooth to move horizontally, and the rotary clamping member 24 can clamp the tooth and drive the tooth to rotate;

[0048] Further, when the lifter 21 works, the execution end of the first stepping motor 216 drives the driving lead screw 217 to rotate. The driving lead screw 217 drives the positioning rod 214 to slide in the positioning sleeve 213 through the driving groove 215 connected by the nut to drive the lifting plate 22 to move up and down. During the process of the lifting plate 22 moving up and down, the guide post 212 slides in the guide sleeve 211 to increase the stability of the lifting plate 22 when moving up and down;

[0049] Further, when the planar mover 23 works, the execution end of the first linear module 231 drives the second linear module 232 to move, and the execution end of the second linear module 232 drives the rotary clamping member 24 to move;

[0050] Further, when the rotary clamping member 24 works, the execution end of the first motor 243 drives the bidirectional lead screw 244 to rotate. The bidirectional lead screw 244 drives the two first sliders 242 to approach or separate from each other. When the two first sliders 242 approach each other, the tooth to be processed is pressed between the two suction cups 246 to clamp and fix the tooth. The execution end of the second motor 247 can drive the rotating rod 245 to rotate to rotate the tooth to be processed.

[0051] Please refer specifically to the appendix Figure 2 、 4As shown in FIGS. 7 and 8, in another preferred embodiment of the present invention, the switching type tooth cutting mechanism includes a switching turntable 30 located above the rotating clamping member 24, a grinding member 31, a flushing member 32, a grooving member 33 and a clamping and separating device 40 annularly arranged at the bottom of the switching turntable 30. The clamping and separating device 40 is used to clamp the groove processed on the tooth by the grooving member 33 and separate the tooth into two halves under the drive of the rotating clamping member 24. The grinding member 31 includes an n-shaped frame 311 arranged at the bottom of the switching turntable 30, a grinding roller 312 rotatably connected to the n-shaped frame 311 and horizontally arranged, and a drive motor 313 arranged on the outer wall of the n-shaped frame 311 and used to drive the grinding roller 312 to rotate. The flushing member 32 includes a drive cylinder 321 arranged at the top of the switching turntable 30 and whose execution end penetrates through the switching turntable 30, a finger cylinder 322 arranged at the execution end of the drive cylinder 321, two sealing boxes 323 symmetrically arranged at the execution end of the finger cylinder 322, a water inlet pipe 324 with one end communicating with one of the sealing boxes 323, and a drain pipe 325 with one end communicating with the other sealing box 323. The grooving member 33 includes a power motor 331 arranged at the top of the switching turntable 30 and whose execution end penetrates through the switching turntable 30, an extension rod 332 arranged at the execution end of the power motor 331, and a grooving ball head 333 arranged at the end of the extension rod 332. A second stepping motor 301 is arranged on the base plate 10, and the execution end of the second stepping motor 301 is connected to one end of a drive shaft 302. The other end of the drive shaft 302 penetrates through the lifting plate 22 and is connected to the bottom of the switching turntable 30.

[0052] It should be noted that in this embodiment, the execution end of the second stepping motor 301 can drive the drive shaft 302 to rotate, the drive shaft 302 can drive the switching turntable 30 to rotate, and the switching turntable 30 can move the grinding member 31 or the flushing member 32 or the grooving member 33 or the clamping and separating device 40 to the processing station to process the tooth. When the tooth is ground, the execution end of the drive motor 313 drives the grinding roller 312 to rotate through a gear, and the grinding roller 312 grinds the tooth surface.

[0053] Furthermore, when the tooth is grooved, the execution end of the power motor 331 drives the extension rod 332 to rotate, the extension rod 332 drives the grooving ball head 333 to rotate, and the grooving ball head 333 grooves the tooth surface.

[0054] Further, during the tooth cleaning process, after the execution end of the driving cylinder 321 drives the finger cylinder 322 to descend to a specified position, the execution end of the finger cylinder 322 drives the two sealing boxes 323 to approach each other to form a sealing cavity. The tooth to be processed is located in the sealing cavity. The water source system connected to the water inlet pipe 324 supplies water flow, and the negative pressure system connected to the drain pipe 325 performs negative pressure suction. The water flows into the sealing box 323 through the water inlet pipe 324 to wash and clean the tooth, and the wastewater after washing is discharged through the drain pipe 325.

[0055] Please refer specifically to the attached Figure 2 、 5 、6, and 7. In another preferred embodiment of the present invention, the clamping and separating device 40 includes a track 41 provided at the bottom of the switching turntable 30, two second sliders 42 that are slidably connected to the track 41 and are symmetrically arranged, two first springs 43 symmetrically provided at both ends of the inner wall of the track 41, an automatic card slot component 44 provided at one end of the bottom of the second slider 42, and a magnetic attraction linkage component 45 provided at the other end. The automatic card slot component 44 includes a cross bar 441 provided on the outer wall of the second slider 42, a first card block 4411 provided at the bottom of the cross bar 441, vertical bars 442 symmetrically provided at both ends of the cross bar 441 and hinged to the cross bar 441, a second card block 4421 provided on the side wall of the vertical bar 442, a magnetic block 443 provided on the side of the vertical bar 442 away from the second card block 4421, an n-shaped bar 444 whose bottom is connected to the top of the cross bar 441, and two first electromagnetic blocks 445 symmetrically provided on both sides of the n-shaped bar 444 and abutting against the magnetic block 443. The magnetic attraction linkage component 45 includes a positioning box 451 provided on the outer wall of the second slider 42, a second electromagnetic block 452 whose top is slidably connected to the inner wall of the positioning box 451, a second spring 453 provided at the top of the inner wall of the positioning box 451, two extension plates 454 symmetrically provided on both sides of the positioning box 451, an arc-shaped slide rail 455 provided at the end of the extension plate 454, and a connecting rod 456 whose one end is connected to the outer wall of the vertical bar 442 and the other end is slidably connected to the arc-shaped slide rail 455.

[0056] It should be noted that in this embodiment, during the tooth separation process, the tooth clamping device 20 applies a squeezing force to the tooth to be processed and maintains it for a unit time. After completion, the clamping and separating device 40 clamps the annular groove on the tooth to be processed and applies a separating force to the tooth to be processed through the tooth clamping device 20 and maintains it for a unit time to complete one tooth separation process. The tooth separation process is repeated until the tooth to be processed is separated into two halves;

[0057] When the execution end of the first motor 243 drives the two first sliders 242 to separate from each other through the bidirectional lead screw 244, a separating force is applied to the teeth to be processed. When the execution end of the first motor 243 drives the two first sliders 242 to approach each other through the bidirectional lead screw 244, a squeezing force is applied to the teeth to be processed;

[0058] Further, when the clamping and separating device 40 works, the first spring 43 gives an elastic thrust to the second slider 42, so that the two second sliders 42 are attached to each other in the initial state. When the automatic card slot component 44 engages with the annular groove on the tooth, the tooth clamping device 20 drives the tooth to be processed to move until the first block 4411 is inserted into the annular groove. When the first electromagnet 445 is energized to generate a repulsive force opposite to the magnetic block 443, the repulsive force drives the vertical rod 442 to rotate around the end hinge point until the second block 4421 is inserted into the annular groove to complete the clamping work. During the clamping and positioning process of the tooth clamping device 20, the second electromagnet 452 moves correspondingly in the positioning box 451. After the tooth clamping device 20 completes the clamping and positioning, the second electromagnet 452 electromagnetically attracts the rotating rod 245. When the two first sliders 242 separate from each other, a separating force is given to the end of the vertical rod 442 through the second electromagnet 452, the positioning box 451, the extension plate 454, the arc-shaped slide rail 455 and the connecting rod 456, and a separating force is given to the cross bar 441 through the second electromagnet 452, the positioning box 451 and the second slider 42, so that the force is evenly distributed when the tooth to be processed is separated.

[0059] According to the above embodiments, an extraction method for a dental pulp stem cell extraction device will also be provided, including the following steps:

[0060] Step 1: X-ray photographing of the tooth; taking an X-ray photograph of the tooth to obtain the information of the dental pulp cavity of the tooth;

[0061] Step 2: Grinding treatment of the tooth; the tooth clamping device 20 clamps the tooth, and after clamping, the grinding component 31 grinds the outer surface of the tooth to obtain Tooth 1;

[0062] Step 3: Grooving treatment of the tooth; the grooving component 33 performs a circular grooving on the surface of Tooth 1 according to the dental pulp cavity information to obtain Tooth 2;

[0063] Step 4: Cleaning of the tooth; the rinsing component 32 rinses the surface of Tooth 2 to obtain Tooth 3;

[0064] Step 5: Separation of the tooth; the tooth clamping device 20 applies a squeezing force to Tooth 3 and maintains it for a unit time. After completion, the clamping and separating device 40 clamps the annular groove on Tooth 3 and applies a separating force to the tooth through the tooth clamping device 20 and maintains it for a unit time to complete one tooth separation process. Repeat the tooth separation process until Tooth 3 is separated into two halves to obtain two Teeth 4;

[0065] Step 6: Extraction of dental pulp tissue; Use forceps to peel the dental pulp tissue from two teeth four and place it in sterile PBS;

[0066] Step 7: Digestion of dental pulp tissue; Cut the dental pulp tissue into pieces and mix it with preheated digestive enzyme, and perform water bath digestion for a unit time to obtain digestive fluid one;

[0067] Step 8: Preparation of cell suspension; Add an equal volume of complete medium to digestive fluid one, and after mixing evenly, perform centrifugation. After centrifugation is completed, remove the supernatant, and resuspend the cells with dental pulp mesenchymal stem cell complete medium;

[0068] Step 9: Inoculation and culture; Inoculate the resuspended cells into a culture dish and place it in an incubator for culture to complete the extraction of dental pulp stem cells.

[0069] The working principle of the present invention is as follows:

[0070] All electrical components in the present invention operate starting from the controller.

[0071] When extracting dental pulp stem cells, it is necessary to first take an X-ray of the tooth. The X-ray picture information after shooting is uploaded to the controller. After the controller analyzes it, the dental pulp cavity information of the tooth is obtained. After obtaining the dental pulp cavity information, it is necessary to perform grinding treatment, grooving treatment, cleaning treatment, and separation treatment on the tooth in sequence to separate the tooth and fully expose the dental pulp cavity. After the dental pulp cavity is fully exposed, use forceps to peel the dental pulp tissue from two teeth half and place it in sterile PBS. Cut the dental pulp tissue into pieces and mix it with preheated digestive enzyme. After water bath digestion for a unit time, add an equal volume of complete medium, and after mixing evenly, perform centrifugation. After centrifugation is completed, remove the supernatant, and resuspend the cells with dental pulp mesenchymal stem cell complete medium. Inoculate the resuspended cells into a culture dish and place it in an incubator for culture to complete the extraction of dental pulp stem cells;

[0072] When the tooth clamping device 20 clamps the tooth, the lifter 21 can drive the tooth to rise and fall, the planar mover 23 can drive the tooth to move planar, and the rotating clamping member 24 can clamp the tooth and drive the tooth to rotate;

[0073] When the lifter 21 works, the execution end of the first stepping motor 216 drives the driving lead screw 217 to rotate. The driving lead screw 217 drives the positioning rod 214 to slide in the positioning sleeve 213 through the driving groove 215 connected by the nut to drive the lifting plate 22 to rise and fall. During the rising and falling process of the lifting plate 22, the guide post 212 slides in the guide sleeve 211 to increase the stability of the lifting plate 22 when rising and falling;

[0074] When the planar mover 23 is working, the execution end of the first linear module 231 drives the second linear module 232 to move, and the execution end of the second linear module 232 drives the rotating clamping member 24 to move;

[0075] When the rotating clamping member 24 is working, the execution end of the first motor 243 drives the bidirectional lead screw 244 to rotate. The bidirectional lead screw 244 drives the two first sliders 242 to approach or separate from each other. When the two first sliders 242 approach each other, the tooth to be processed is pressed between the two suction cups 246 to clamp and fix the tooth. The execution end of the second motor 247 can drive the rotating rod 245 to rotate to rotate the tooth to be processed;

[0076] The execution end of the second stepping motor 301 can drive the drive shaft 302 to rotate. The drive shaft 302 can drive the switching turntable 30 to rotate. The switching turntable 30 can move the grinding member 31 or the flushing member 32 or the grooving member 33 or the clamping and separating device 40 to the processing station to process the tooth. When the tooth is ground, the execution end of the drive motor 313 drives the grinding roller 312 to rotate through the gear, and the grinding roller 312 grinds the tooth surface;

[0077] When the tooth is grooved, the execution end of the power motor 331 drives the extension rod 332 to rotate. The extension rod 332 drives the grooving ball head 333 to rotate, and the grooving ball head 333 grooves the tooth surface;

[0078] When the tooth is cleaned, after the execution end of the drive cylinder 321 drives the finger cylinder 322 to descend to the specified position, the execution end of the finger cylinder 322 drives the two sealing boxes 323 to approach each other to form a sealed cavity. The tooth to be processed is located in the sealed cavity. The water source system connected to the water inlet pipe 324 supplies water flow, and the negative pressure system connected to the drain pipe 325 performs negative pressure suction. The water flows into the sealing box 323 through the water inlet pipe 324 to wash and clean the tooth, and the waste water after washing is discharged through the drain pipe 325;

[0079] When the tooth is separated, the tooth clamping device 20 applies an extrusion force to the tooth to be processed and maintains it for a unit time. After completion, the clamping and separating device 40 clamps the annular groove on the tooth to be processed and applies a separating force to the tooth to be processed through the tooth clamping device 20 and maintains it for a unit time to complete one tooth separation process. The tooth separation process is repeated until the tooth to be processed is separated into two halves;

[0080] When the execution end of the first motor 243 drives the two first sliders 242 to separate from each other through the bidirectional lead screw 244, a separating force is applied to the tooth to be processed. When the execution end of the first motor 243 drives the two first sliders 242 to approach each other through the bidirectional lead screw 244, an extrusion force is applied to the tooth to be processed;

[0081] When the clamping and separating device 40 is working, the first spring 43 gives an elastic thrust to the second slider 42, so that the two second sliders 42 are in contact with each other in the initial state. When the automatic card slot component 44 engages with the annular groove on the tooth, the tooth clamping device 20 drives the tooth to be processed to move until the first clamping block 4411 is inserted into the annular groove. The first electromagnetic block 445 is energized to generate a repulsive force opposite to the magnetic block 443, and the repulsive force drives the vertical rod 442 to rotate around the end hinge point until the second clamping block 4421 is inserted into the annular groove to complete the clamping work. During the process of the tooth clamping device 20 being clamped and positioned, the second electromagnetic block 452 moves correspondingly in the positioning box 451. After the tooth clamping device 20 is clamped and positioned, the second electromagnetic block 452 electromagnetically attracts the rotating rod 245. When the two first sliders 242 are separated from each other, a separating force is given to the end part of the vertical rod 442 through the second electromagnetic block 452, the positioning box 451, the extension plate 454, the arc-shaped slide rail 455 and the connecting rod 456, and a separating force is given to the cross bar 441 through the second electromagnetic block 452, the positioning box 451 and the second slider 42, so that the tooth to be processed is evenly stressed when separated.

[0082] Although the embodiments of the present invention have been shown and described, the specific embodiments are only explanations of the present invention and are not limitations to the invention. The specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art can make modifications, substitutions and variations without creative contributions to the embodiments according to needs, but as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A dental pulp stem cell extraction device, comprising a base plate (10), a tooth clamping device (20) disposed on the base plate (10), and a switchable tooth cutting mechanism located on the upper part of the tooth clamping device (20), characterized in that: The tooth clamping device (20) comprises a lifter (21) arranged on the base plate (10), a lifting plate (22) arranged at the execution end of the lifter (21), a plane mover (23) arranged on the top of the lifting plate (22), and a rotating clamping component (24) arranged at the execution end of the plane mover (23); The switchable tooth cutting mechanism comprises a switchable turntable (30) located on the upper part of a rotating clamping component (24), a grinding component (31), a flushing component (32), a groove cutting component (33) and a clamping and separating device (40) arranged in an annular array at the bottom of the switchable turntable (30), wherein the clamping and separating device (40) is used to clamp the groove machined on the tooth by the groove cutting component (33) and separate the tooth into two halves under the drive of the rotating clamping component (24); The engaging and separating device (40) comprises a track (41) arranged at the bottom of the switching turntable (30), two second sliders (42) slidably connected to the track (41) and symmetrically arranged, two first springs (43) symmetrically arranged at two ends of the inner wall of the track (41), and an automatic slot component (44) arranged at one end of the bottom of the second slider (42) and a magnetic attraction linkage component (45) at the other end, wherein the automatic slot component (44) comprises a cross bar (441) arranged on the outer wall of the second slider (42), and a first spring (43) symmetrically arranged at two ends of the inner wall of the track (41). ), a first clamping block (4411) at the bottom of the crossbar (441), a vertical bar (442) symmetrically arranged at both ends of the crossbar (441) and hinged to the crossbar (441), a second clamping block (4421) arranged on the side wall of the vertical bar (442), a magnetic block (443) arranged on the side of the vertical bar (442) away from the second clamping block (4421), an n-shaped bar (444) connected to the top of the crossbar (441) at the bottom, and two first electromagnetic blocks (445) symmetrically arranged on both sides of the n-shaped bar (444) and abutting against the magnetic block (443).

2. A dental pulp stem cell extraction device according to claim 1, characterized in that: The rotating clamping component (24) comprises a base plate (241) arranged at the execution end of the plane mover (23), two first sliders (242) slidably connected to the top of the base plate (241) and symmetrically arranged, a first motor (243) arranged at the top of the base plate (241), a bidirectional screw rod (244) arranged at the execution end of the first motor (243) and connected to the nut of the first slider (242), a rotating rod (245) rotatably connected to the top of the side wall of the first slider (242), a suction cup (246) arranged at the end of the rotating rod (245), and a second motor (247) arranged on one of the first sliders (242) and used to drive the rotating rod (245) to rotate.

3. The dental pulp stem cell extraction device according to claim 1, characterized in that: The grinding component (31) comprises an n-shaped frame (311) arranged at the bottom of the switching turntable (30), a grinding roller (312) rotatably connected to the n-shaped frame (311) and arranged horizontally, and a driving motor (313) arranged on the outer wall of the n-shaped frame (311) and used for driving the grinding roller (312) to rotate.

4. The dental pulp stem cell extraction device according to claim 1, characterized in that: The flushing component (32) comprises a driving cylinder (321) disposed at the top of the switching turntable (30) and having an execution end passing through the switching turntable (30), a finger cylinder (322) disposed at the execution end of the driving cylinder (321), two sealing boxes (323) symmetrically disposed at the execution end of the finger cylinder (322), a water inlet pipe (324) having one end connected to one of the sealing boxes (323), and a drainage pipe (325) having one end connected to the other of the sealing boxes (323).

5. The dental pulp stem cell extraction device according to claim 1, characterized in that: The groove cutting component (33) comprises a power motor (331) disposed on the top of the switching turntable (30) and having an execution end passing through the switching turntable (30), an extension rod (332) disposed on the execution end of the power motor (331), and a grooved ball head (333) disposed on the end of the extension rod (332).

6. The dental pulp stem cell extraction device according to claim 1, characterized in that: The magnetic attraction linkage component (45) comprises a positioning box (451) arranged on the outer wall of the second sliding block (42), a second electromagnetic block (452) slidably connected to the inner wall of the positioning box (451) at the top, a second spring (453) arranged at the top of the inner wall of the positioning box (451), two extension plates (454) symmetrically arranged on both sides of the positioning box (451), an arc-shaped slide rail (455) arranged at the end of the extension plate (454), and a connecting rod (456) having one end connected to the outer wall of the vertical rod (442) and the other end slidably connected to the arc-shaped slide rail (455).

7. The dental pulp stem cell extraction device according to claim 2, characterized in that: The lifter (21) comprises a plurality of guide sleeves (211) arranged on the base plate (10), a guide column (212) whose bottom is slidably connected to the inner wall of the guide sleeves (211) and whose top is connected to the bottom of the lift plate (22), a positioning sleeve (213) arranged at the center of the top of the base plate (10), a positioning rod (214) whose bottom is slidably connected to the inner wall of the positioning sleeve (213) and whose top is connected to the bottom of the lift plate (22), a driving groove (215) embedded in the bottom of the positioning rod (214), a first stepper motor (216) arranged at the bottom of the base plate (10), and a driving screw rod (217) arranged at the execution end of the first stepper motor (216) and connected to the nut of the driving groove (215); The plane mover (23) comprises a first linear module (231) arranged on the top of the lifting plate (22), and a second linear module (232) arranged at an execution end of the first linear module (231) and distributed perpendicularly to the first linear module (231), wherein the execution end of the second linear module (232) is connected to the bottom of the base plate (241).

8. The dental pulp stem cell extraction device according to claim 1, characterized in that: A second stepper motor (301) is provided on the base plate (10), the execution end of the second stepper motor (301) is connected to one end of a driving shaft (302), and the other end of the driving shaft (302) passes through the lifting plate (22) and is connected to the bottom of the switching turntable (30).

9. The extraction method of the dental pulp stem cell extraction device according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step 1: X-ray the teeth to obtain information about the dental pulp cavity; Step 2: grinding the tooth. The tooth clamping device (20) clamps the tooth. After the clamping is completed, the grinding component (31) grinds the outer surface of the tooth to obtain tooth 1. Step 3: tooth grooving: the grooving component (33) performs an annular grooving on the surface of tooth 1 according to the pulp cavity information to obtain tooth 2; Step 4: cleaning the teeth, the flushing component (32) flushes the surface of the second tooth to obtain the third tooth; Step 5, separation of the teeth, the tooth clamping device (20) applies a squeezing force to the tooth three and maintains the squeezing force for a unit time, after completion, the clamping separation device (40) clamps the annular groove on the tooth three and applies a separation force to the tooth through the tooth clamping device (20) and maintains the separation force for a unit time, so as to complete a tooth separation process, and the tooth separation process is repeated until the tooth three is separated into two halves, so as to obtain two teeth four; Step 6: Extraction of dental pulp tissue: Use forceps to peel off the dental pulp tissue from two teeth and place it in sterile PBS; Step 7: Digestion of dental pulp tissue: mince the dental pulp tissue, mix it with the preheated digestive enzyme, and digest it in a water bath for a unit time to obtain digestion solution 1; Step 8, preparation of cell suspension, adding an equal volume of complete culture medium to digestion solution 1, and centrifuging after mixing evenly, removing the supernatant after centrifugation, and resuspending the cells in complete culture medium for dental pulp mesenchymal stem cells; Step nine: inoculation and culture. The resuspended cells are inoculated into a culture dish and placed in an incubator for culture to complete the extraction of dental pulp stem cells.

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