Reagent needle structure, reagent storage module and biochemical reaction equipment
By designing a reagent needle structure including a reagent needle assembly, a driving plate and an elastic assembly, the existing reagent needle is easily damaged and has large sampling errors during the sampling process, automatic scaling adjustment is achieved, damage risk and error are reduced, and sequencing quality is improved.
Patent Information
- Application Number
- CN202311397848.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-25
- Publication Date
- 2025-04-25
AI Technical Summary
During the sampling and loading of existing reagent needles, the reagent needles or instruments are easily damaged, resulting in sequencing failure, and the sampling error is large, affecting the sequencing quality.
A reagent needle structure is designed, including a reagent needle assembly, a driving plate and an elastic assembly. By setting the reagent needle assembly and the driving plate to be relatively movable, and adding an elastic assembly between the reagent needle assembly and the driving plate, a flexible connection is achieved, allowing the reagent needle assembly to automatically telescopic and adjust during the liquid extraction process.
The automatic telescopic reagent needle structure reduces the error rate of mutual coordination between the reagent needle structure and reagent storage instruments such as reagent kits, avoids damage to the reagent needle and the instrument, ensures the smooth progress of the biochemical reaction, and improves the accuracy of reagent extraction.
Smart Images
Figure CN119875802A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of liquid transfer technology, and in particular to a reagent needle structure, a reagent storage module and a biochemical reaction device. Background Art
[0002] With the continuous development of sequencing technology, gene sequencing has broad application prospects in the fields of early disease screening, microbiology, genetic testing, etc., and the market demand for sequencers is also increasing. As the core module of the gene sequencer, the reagent storage module, its core component is the reagent needle used to extract the sequencing reagent in the kit.
[0003] The existing reagent needles are prone to damage to the reagent needles or instruments during the sampling and adding process, resulting in sequencing failure; moreover, the existing reagent needles have large sampling errors, affecting the sequencing quality. Summary of the invention
[0004] In order to solve at least one of the above defects, it is necessary to propose a reagent needle structure, a reagent storage module and a biochemical reaction device.
[0005] The embodiment of the present application provides a reagent needle structure, which includes: a reagent needle assembly, a driving plate and an elastic assembly, wherein the reagent needle assembly includes a reagent needle body and a reagent needle fixing plate connected to the reagent needle body; the reagent needle body can movably pass through the driving plate, and the driving plate and the reagent needle fixing plate are relatively movably arranged; the elastic assembly is arranged between the reagent needle fixing plate and the driving plate, wherein the driving plate is used to drive the reagent needle assembly to move along a first direction under the drive of an external force, and the reagent needle body, under the action of a force along a second direction, drives the reagent needle fixing plate to move relative to the driving plate along the second direction to compress the elastic assembly; the driving plate is also used to drive the reagent needle assembly to move along the second direction under the drive of an external force, so as to restore the elastic assembly and reset the reagent needle assembly, wherein the first direction is opposite to the second direction, and the first direction is the extension direction of the reagent needle body.
[0006] In some possible embodiments, the reagent needle fixing plate is fixedly connected to the reagent needle body.
[0007] In some possible embodiments, the reagent needle body includes a fluid tube and a sleeve sleeved on the outside of the fluid tube, the sleeve and the fluid tube are an integrated structure, and the sleeve is fixedly connected to the reagent needle fixing plate, so that the reagent needle body is fixedly connected to the reagent needle fixing plate.
[0008] In some possible embodiments, the sleeve is made of metal.
[0009] In some possible embodiments, the sleeve and the reagent needle fixing plate are connected together by welding.
[0010] In some possible embodiments, the reagent needle body includes a liquid collection end, and the fluid tube of the liquid collection end is sealedly connected to the sleeve.
[0011] In some possible embodiments, the reagent needle assembly further includes a pipe connector disposed at one end of the reagent needle body.
[0012] In some possible embodiments, the elastic component includes a plurality of elastic members, and the plurality of elastic members are arranged around the reagent needle body, and two ends of each of the elastic members are respectively connected to the reagent needle fixing plate and the driving plate.
[0013] An embodiment of the present application further provides a reagent storage module, which includes a reagent kit, a driving mechanism, and the reagent needle structure as described above, wherein the driving mechanism is used to drive the driving plate to drive the reagent needle assembly to extract liquid from the reagent kit.
[0014] The embodiment of the present application also provides a biochemical reaction device, which includes a reagent use module and the reagent storage module as described above, and the reagent needle body is connected to the reagent use module to add the reagent extracted from the reagent kit to the reagent use module.
[0015] In the above reagent needle structure provided by the embodiment of the present application, by setting the reagent needle assembly and the driving plate to be relatively movable, and adding an elastic component between the reagent needle assembly and the driving plate, a flexible connection is achieved between the reagent needle assembly and the driving plate, so that the reagent needle assembly can be automatically telescopically adjusted during the liquid extraction process, reducing the error rate of the mutual coordination between the reagent needle structure and the reagent storage instrument such as the test kit caused by assembly and processing errors; and when the reagent needle assembly moves excessively along the first direction, it can automatically retract, and a telescopic reagent needle structure is obtained. During the liquid extraction process, the reagent needle assembly and the instrument such as the test kit will not have a rigid collision, reducing the risk of damage to the reagent needle body and the reagent storage instrument, and ensuring the smooth progress of the biochemical reaction; the automatically telescopic reagent needle assembly can improve the accuracy of reagent extraction. The structure of the automatic telescopic reagent needle structure is simple, the processing difficulty of each part is low, it is easy to form, and the cost is low. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments of the present invention will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0017] Figure 1 It is a system block diagram of a biochemical reaction device provided in one embodiment of the present application.
[0018] Figure 2 It is a structural schematic diagram of a reagent needle structure provided in one embodiment of the present application.
[0019] Figure 3 yes Figure 2 Schematic diagram of the structure of the liquid extraction end.
[0020] Figure 4 It is a schematic diagram of the structure of the reagent needle structure extending into the reagent kit in one embodiment of the present application.
[0021] Figure 5 It is a schematic structural diagram of the reagent needle assembly retracting in the reverse direction after the liquid collection end presses against the bottom of the reagent reagent box when the reagent needle assembly moves beyond the lower limit in one embodiment of the present application.
[0022] Figure 6 It is a schematic structural diagram of an embodiment of the present application in which a driving plate drives the reagent needle assembly to move upward, and the reagent needle assembly and the elastic member are reset.
[0023] Main component symbols
[0024] Biochemical reaction equipment 1000
[0025] Reagent storage module 100
[0026] Reagent needle structure 10
[0027] Reagent needle assembly 1
[0028] Reagent needle body 4
[0029] Fluid pipe 41
[0030] Casing 42
[0031] Liquid taking end 43
[0032] Sealing portion 44
[0033] Reagent needle fixing plate 5
[0034] Driver board 2
[0035] Through hole 21
[0036] Elastic part 3
[0037] Guide column 31
[0038] Spring 32
[0039] Pipeline connector 6
[0040] Aluminum sealing membrane 7
[0041] Kit 20
[0042] Driving mechanism 30
[0043] The first direction a
[0044] The second direction b
[0045] Reagent Use Module 200
[0046] The following specific implementation manner will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION
[0047] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0048] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be a central component at the same time. The term "and / or" as used herein includes all and any combinations of one or more of the relevant listed items.
[0049] When the reagent needle in the existing gene sequencer extracts the reagent, it is easy to cause damage to the reagent needle or the instrument, resulting in sequencing failure; in addition, the existing reagent needle has a large sampling error, which affects the sequencing quality.
[0050] In order to solve the above problems, the embodiment of the present application provides a reagent needle structure, which can be used in the reagent storage module of the gene sequencer to extract reagents from the reagent kit. The use of the reagent needle structure provided by the embodiment of the present application can effectively reduce the risk of damage to the reagent needle and the instrument and sequencing failure, and can improve the accuracy of sampling and improve the sequencing quality. It is understandable that the reagent needle structure can also be used in other biochemical reaction equipment to extract reagents.
[0051] See also Figure 1The embodiment of the present application provides a biochemical reaction device 1000, which includes: a reagent storage module 100 and a reagent usage module 200, wherein the reagent storage module 100 is used to add reagents required for a specific biochemical reaction (such as reagents required for gene sequencing) to the reagent usage module 200, and the reagent usage module 200 is used to perform biochemical reactions, such as gene sequencing reactions, etc.
[0052] The reagent storage module 100 includes a reagent needle structure 10, a reagent kit 20 and a driving mechanism 30, wherein the driving mechanism 30 is used to drive the reagent needle structure 10 to extract the reagent in the reagent kit 20. Specifically, the reagent needle structure 10 is connected to the reagent use module 200, wherein the driving mechanism 30 drives the reagent needle structure 10 to extend into the reagent kit 20 and extract the required reagent from the reagent kit 20, and the reagent extracted from the reagent needle structure 10 will further enter the reagent use module 200 to complete the biochemical reaction.
[0053] Please refer to Figure 2 , the reagent needle structure 10 provided in the embodiment of the present application includes: a reagent needle assembly 1, a driving plate 2 and an elastic assembly, the reagent needle assembly 1 includes a reagent needle body 4 and a reagent needle fixing plate 5 connected to the reagent needle body 4; the reagent needle body 4 can movably pass through the driving plate 2, and the driving plate 2 and the reagent needle fixing plate 5 are relatively movable. The elastic assembly is arranged between the reagent needle fixing plate 5 and the driving plate 2. Wherein, the driving plate 2 is used to drive the reagent needle assembly 1 to move along the first direction a under the drive of an external force, and the reagent needle body 4 drives the reagent needle fixing plate 5 to move relative to the driving plate 2 along the second direction b when it is acted upon by a force along the second direction b, so as to compress the elastic assembly. The driving plate 2 is also used to drive the reagent needle assembly 1 to move along the second direction b under the drive of an external force, so as to restore the elastic assembly and reset the reagent needle assembly 1, wherein the first direction a is opposite to the second direction b, and the first direction a is the extension direction of the reagent needle body 4.
[0054] Specifically, the driving plate 2 is connected to the driving mechanism 30 of the reagent storage module 100 , and the movement of the driving plate 2 is achieved through the driving mechanism 30 , thereby achieving the movement of the reagent needle assembly 1 .
[0055] The reagent needle fixing plate 5 and the reagent needle body 4 can be fixedly connected to form an integrated structure. The reagent needle fixing plate 5 and the reagent needle body 4 are designed as an integrated structure, which can realize linkage, so as to realize automatic retraction and automatic reset of the reagent needle body 4.
[0056] In some embodiments, the reagent needle body 4 includes a fluid tube 41 and a sleeve 42 sleeved on the outside of the fluid tube 41, the sleeve 42 and the fluid tube 41 are an integrated structure, and the sleeve 42 and the reagent needle fixing plate 5 can be fixedly connected, so that the reagent needle body 4 is fixedly connected to the reagent needle fixing plate 5 to form an integrated structure. By sleeve-arranging the sleeve 42 on the outside of the fluid tube 41, the inner fluid tube 41 can be protected to improve the strength of the overall reagent needle body 4, so as to facilitate piercing the sealing film at the opening of the reagent test kit 20; and the fluid tube 41 and the sleeve 42 are designed as an integrated structure, so that the fluid tube 41 and the sleeve 42 can be linked. In addition, by setting the sleeve 42, it is convenient to be fixedly connected with the reagent needle fixing plate 5 to form an integrated structure. It can be understood that the reagent needle fixing plate 5 and the sleeve 42 can also be detachably connected.
[0057] In certain embodiments, the material of the fluid tube 41 can be plastic (e.g., fluorinated ethylene propylene copolymer, FEP), or materials such as glass. It is understandable that the material of the fluid tube 41 should be selected to be not easily corroded by the extracted reagent. Specifically, the fluid tube 41 in the reagent needle body 4 is communicated with the reagent use module 200, and when the fluid tube 41 is made of plastic, the soft plastic fluid tube 41 can be bent arbitrarily, which is convenient for connection with the reagent use module 200, and makes reasonable use of space. It is understandable that when the fluid tube 41 adopts a glass tube, a soft hose can be used to connect the fluid pipeline 41 and the reagent use module 200.
[0058] In some embodiments, the sleeve 42 may be made of metal. The use of the metal sleeve 42 can further improve the rigidity and hardness of the fluid tube 41. It is understandable that the sleeve 42 may also be made of hard plastic.
[0059] In some embodiments, when the sleeve 42 is made of metal, the sleeve 42 and the reagent needle fixing plate 5 are connected together by welding. The welding method is simple, which facilitates the reagent needle body 4 and the reagent needle fixing plate 5 to form an integrated structure. It can be understood that when the sleeve 42 is made of hard plastic, the sleeve 42 and the reagent needle fixing plate 5 can also be connected in an integrated manner by welding or bonding.
[0060] In some embodiments, the material of the reagent needle fixing plate 5 may also be metal or other hard plastics.
[0061] Please refer to Figure 3 The fluid tube 41 includes a liquid collection end 43, and the liquid collection end 43 is tilted to form an oblique opening with a sharp tip, which is convenient for piercing the sealing film of the reagent kit 20 to extract the reagent.
[0062] In some embodiments, the sleeve 42 extends to the liquid collection end 43, which can improve the rigidity and hardness of the liquid collection end 43, so that the oblique mouth of the liquid collection end 43 has sufficient hardness to facilitate puncturing the sealing film on the reagent kit 20. At the liquid collection end 43, the sleeve 42 is sealed and connected with the fluid tube 41 to form an integrated structure. After the sealing treatment, during the process of extracting the reagent, the reagent can enter the fluid tube 41 smoothly, and the reagent can also be effectively prevented from entering the gap between the fluid tube 41 and the sleeve 42, thereby improving the accuracy of reagent extraction. The sealing treatment of the sleeve 42 and the fluid tube 41 at the liquid collection end 43 can be achieved by filling the sealing material to form the sealing part 44, or by bending the end of the fluid tube 41 toward the sleeve 42 and hot-melting to achieve sealing, or other sealing treatment methods that can achieve sealing between the fluid tube 41 and the sleeve 42 can be adopted.
[0063] In some embodiments, the reagent needle assembly 1 further includes a pipe connector 6 disposed at one end of the reagent needle body 4 , which can achieve communication with the reagent use module 200 .
[0064] Please refer again Figure 2 The elastic component includes one or more elastic members 3. Specifically, in this embodiment, the elastic component includes a plurality of elastic members 3 arranged around the reagent needle body 4, and the two ends of each elastic member 3 are respectively connected to the reagent needle fixing plate 5 and the driving plate 2. By arranging a plurality of elastic members 3 around the actual needle body, the stability of the telescopic movement of the reagent needle body 4 can be improved.
[0065] In some embodiments, the elastic member 3 includes a guide column 31 and a spring 32 sleeved on the guide column 31, one end of the guide column 31 is fixed on the driving plate 2, and the other end passes through the reagent needle fixing plate 5, and the two ends of the spring 32 are against the opposite surfaces of the reagent needle fixing plate 5 and the driving plate 2.
[0066] Please refer again Figure 2 A through hole 21 is provided at the approximate middle of the driving plate 2, and the reagent needle body 4 is arranged through the through hole 21, and there is a gap between the reagent needle body 4 and the hole wall of the through hole 21, so that the reagent needle body 4 can move freely. A plurality of elastic members 3 are arranged around the through hole 21 and connect the driving plate 2 and the reagent needle fixing plate 5 together.
[0067] The assembly process of the reagent needle structure 10 can be: first weld the sleeve 42 and the reagent needle fixing plate 5 into one body to assemble the reagent needle assembly 1 into an integrated reagent needle; pass the reagent needle body 4 through the through hole 21 of the driving plate 2, and then use the guide column 31 (such as fixing bolts and other parts) and the spring 32 to connect the reagent needle assembly 1 and the driving plate 2 together.
[0068] The improved retractable reagent needle structure 10 in the embodiment of the present application mainly utilizes the elastic characteristics of the elastic component. When the reagent needle body 4 moves along the first direction a (defined as downward), after the needle tip contacts the bottom of the reagent reagent 20, and under the reaction force of the bottom of the reagent reagent 20, the elastic member 3 begins to compress, providing a buffer distance for the reagent needle body 4 so that it no longer continues to move, ensuring that it will not be damaged due to a hard collision with the bottom of the reagent reagent 20. When the reagent extraction is completed, the retractable reagent needle structure 10 moves along the second direction b (defined as upward), the needle tip separates from the bottom of the reagent reagent 20, the reaction force of the reagent reagent 20 disappears, and under the restoring force of the elastic member 3, the reagent needle fixing plate 5 automatically returns to the initial position, realizing the free retractable function of the reagent needle body 4.
[0069] When using: First step, Figure 4 As shown, the driving plate 2 drives the integrated reagent needle assembly 1 to move downward under the action of an external driving force (i.e., driven by the driving mechanism 30 ), and under the action of the needle tip of the liquid collection end 43 , pierces the aluminum sealing film 7 of the reagent kit 20 and extends into the interior of the reagent kit 20 .
[0070] The second step is, Figure 5 As shown, when the displacement of the driving plate 2 exceeds the limit and continues downward, the needle tip of the liquid collection end 43 will collide with the bottom of the reagent reagent 20. Under the reaction force of the bottom of the reagent reagent 20, the reagent needle fixing plate 5 will compress the spring 32 in the elastic part 3, causing the reagent needle assembly 1 to retract, so that the needle tip of the reagent needle body 4 no longer moves downward, thereby ensuring that the reagent needle body 4 is successfully inserted.
[0071] The third step is Figure 6 As shown, after the reagent extraction is completed, the driving plate 2 moves upward under the action of external force, driving the integrated reagent needle assembly 1 to move upward, and when the needle tip is separated from the bottom of the reagent reagent 20, the spring 32 starts to rebound from the compressed state, and under the action of the restoring force, the reagent needle fixing plate 5 drives the reagent needle body 4 to move downward and return to the initial state, thereby completing a reagent needle puncture and reagent extraction process.
[0072] Since the reagent needle assembly 1 is connected to the reagent use module 200 , after the reagent is extracted into the reagent needle body 4 , it can further enter the reagent use module 200 to complete the subsequent biochemical reaction.
[0073] In the above-mentioned reagent needle structure 10 provided in the embodiment of the present application, by setting the reagent needle assembly 1 and the driving plate 2 to be relatively movable, and adding an elastic component between the reagent needle assembly 1 and the driving plate 2, a flexible connection is achieved between the reagent needle assembly 1 and the driving plate 2, so that the reagent needle assembly 1 can be automatically retracted and adjusted during the liquid extraction process, reducing the error rate of the mutual coordination between the reagent needle structure 10 and the reagent storage instrument such as the reagent test kit 20 caused by assembly and processing errors; and when the reagent needle assembly 1 moves excessively along the first direction a, it can be automatically retracted to obtain a telescopic reagent needle structure, and during the liquid extraction process, the reagent needle assembly 1 and the instrument such as the reagent test kit 20 will not have a rigid collision, reducing the risk of damage to the reagent needle body 4 and the reagent test kit 20, and can ensure the smooth progress of the biochemical reaction, improve the reliability of the reagent needle structure 10 and the success rate of the biochemical reaction; the automatically retractable reagent needle assembly 1 can improve the accuracy of reagent extraction. The structure of the automatic retractable reagent needle structure 10 is simple, the processing difficulty of each part is low, it is easy to form, and the cost is low.
[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.
Claims
1. A reagent needle structure, characterized in that: include: A reagent needle assembly, comprising a reagent needle body and a reagent needle fixing plate connected to the reagent needle body; a driving plate, through which the reagent needle body can movably pass, and the driving plate and the reagent needle fixing plate are movably arranged relative to each other; and The elastic component is arranged between the reagent needle fixing plate and the driving plate, Wherein, the driving plate is used to drive the reagent needle assembly to move along the first direction under the drive of external force, and the reagent needle body, under the action of the force along the second direction, drives the reagent needle fixing plate to move relative to the driving plate along the second direction to compress the elastic component; the driving plate is also used to drive the reagent needle assembly to move along the second direction under the drive of external force to restore the elastic component and reset the reagent needle assembly, wherein the first direction is opposite to the second direction, and the first direction is the extension direction of the reagent needle body.
2. The reagent needle structure according to claim 1, characterized in that: The reagent needle fixing plate is fixedly connected to the reagent needle body.
3. The reagent needle structure according to claim 2, characterized in that: The reagent needle body includes a fluid tube and a sleeve sleeved outside the fluid tube, the sleeve and the fluid tube are an integrated structure, and the sleeve is fixedly connected to the reagent needle fixing plate, so that the reagent needle body is fixedly connected to the reagent needle fixing plate.
4. The reagent needle structure according to claim 3, characterized in that: The sleeve is made of metal.
5. The reagent needle structure according to claim 4, characterized in that: The sleeve and the reagent needle fixing plate are connected together by welding.
6. The reagent needle structure according to claim 3, characterized in that: The reagent needle body comprises a liquid collection end, and the fluid tube of the liquid collection end is sealedly connected to the sleeve.
7. The reagent needle structure according to claim 1, characterized in that: The reagent needle assembly also includes a pipeline connector arranged at one end of the reagent needle body.
8. The reagent needle structure according to claim 1, characterized in that: The elastic component includes a plurality of elastic members, and the plurality of elastic members are arranged around the reagent needle body, and two ends of each of the elastic members are respectively connected to the reagent needle fixing plate and the driving plate.
9. A reagent storage module, characterized in that: It comprises a reagent kit, a driving mechanism and a reagent needle structure as described in any one of claims 1 to 8, wherein the driving mechanism is used to drive the driving plate to drive the reagent needle assembly to extract liquid in the reagent kit.
10. A biochemical reaction device, characterized in that: It comprises a reagent using module and the reagent storage module as claimed in claim 9, wherein the reagent needle body is connected to the reagent using module to add the reagent extracted from the reagent kit into the reagent using module.