Analyzer for meat microorganism detection

Abstract

The invention relates to the technical field of meat food detection, and discloses an analyzer for meat microbiological detection, which comprises a primer reagent storage module, a transfer table assembly, a determination system, a sample dropping module and a disposable sample plate which are arranged in a machine body, the transfer table assembly comprises a rotation control mechanism, a rotating disc, a temperature control mechanism and an air pressure control mechanism, the rotating disc is provided with a plurality of fixing protrusions used for fixing the disposable sample plate, the temperature control mechanism comprises heating pieces, the heating pieces are fixedly arranged at the fixing protrusions, and the air pressure control mechanism is arranged on the rotating disc. The primer reagent storage module is communicated with the sample dropping module, a detection analyzer for detecting meat bacteria by using a loop-mediated isothermal amplification technology is designed, compared with a traditional bacterial colony detection method, the device has the advantages that the detection period is greatly shortened, and for meat which is a food with a short shelf life, the detection efficiency is greatly improved; and the direct economic loss caused by the detection period can be effectively reduced.

Description

Technical Field

[0001] The invention relates to the technical field of meat food detection, and in particular to an analyzer for meat microbial detection. Background Art

[0002] Meat and meat products are rich in nutrition and can provide most of the nutrients needed by the human body, but they are also susceptible to microbial contamination and have a short shelf life. Their food safety has attracted much attention. At present, the microbial detection of meat is usually carried out by preparing dilution tubes and culture media in advance, and then processing the sampled samples. Then, the test results can be obtained after dilution, sampling, culturing, counting and other steps. In particular, the culturing step is very time-consuming. Generally, the detection time is relatively short, such as coliform group detection, which takes 2-3 days, while the detection of foodborne pathogens takes 3-5 days. The operation is time-consuming and labor-intensive, which will cause large direct economic losses to meat with a short shelf life.

[0003] The use of loop-mediated isothermal amplification (LAMP) can greatly improve the quantitative detection time of bacteria. The LAMP technology can shorten the detection speed of traditional culture dish colony detection from at least two days to one hour, greatly improving the detection rate. However, loop-mediated isothermal amplification technology is almost always a single test, that is, only one specific primer reagent is added to each test tube, and the target is amplified by a specific reagent to complete the detection. In meat microbial detection, the target microorganisms that need to be detected include Salmonella, Staphylococcus aureus, Listeria monocytogenes, etc. Therefore, when conducting meat testing, it is necessary to use multiple test tubes and add different types of primer reagents to the test tubes, so as to judge whether the microbial pathogen is contained or whether the content of the microbial pathogen exceeds the standard from the test results of different test tubes. This will cause a large workload, and when making different test tubes, it is easy to cause errors in the test results due to human subjective factors. Summary of the invention

[0004] The present invention aims to provide an analyzer for meat microorganism detection, so as to solve the problem that the existing meat microorganism detection is complicated to operate and takes a long time to detect.

[0005] The present invention is achieved through the following technical solutions:

[0006] An analyzer for meat microorganism detection includes a primer reagent storage module, a conversion table assembly, a measurement system, a sample dropping module and a disposable sample plate arranged in a body, the conversion table assembly includes a rotation control mechanism, a rotating disk, a temperature control mechanism and an air pressure control mechanism, the rotating disk is provided with a plurality of fixing protrusions for fixing the disposable sample plate, the temperature control mechanism includes a heating plate, and the heating plate is fixed at the fixing protrusions, the primer reagent storage module is connected to the sample dropping module, the measurement system includes a fluorescence meter, and after the isothermal amplification reaction in the disposable sample plate is completed, the fluorescence meter is used to read the test results in the disposable sample plate.

[0007] In a possible design, an operation display panel is fixedly provided on the top of the body, a primer storage cover and a detection port cover are provided on one side of the body, a primer reagent storage module is provided below the primer storage cover, and the detection port cover is a sample dropping port.

[0008] In one possible design, the disposable sample plate is provided with a plurality of reaction chambers, each of which is used for dripping different primer reagents to detect different target bacteria, the reaction chamber is formed by a hard conical wall and a soft membrane at the bottom, and the disposable sample plate also includes an annular groove surrounding all the reaction chambers.

[0009] In one possible design, the air pressure control mechanism includes an air pump and an air duct arranged in the rotating disk, one end of the air duct is connected to the internal cavity formed by the disposable sample plate and the fixed protrusion, and the other end of the air duct is connected to the air inlet of the air pump, and the rotating disk is provided with the same number of air ducts as the fixed protrusions.

[0010] In a possible design, the rotation control mechanism includes a fixed support base fixed in the body and a rotation control motor fixed on the fixed support base, the motor shaft of the rotation control motor is fixed to the center position of the rotating disk, and the fixed support base rotates in coordination with the rotating disk.

[0011] In one possible design, the primer reagent storage module includes a primer reagent cold storage room and a primer reagent storage tube. A plurality of primer reagent storage tubes are fixed in the primer reagent cold storage room, each of the primer reagent storage tubes stores a different primer reagent, and the primer reagent cold storage room is used to store the primer reagents in the primer reagent storage tubes at low temperatures.

[0012] In a possible design, the sample dropping module includes multiple tubes, one end of each of the tubes is connected to the bottom of a primer reagent storage tube, and the other end of the tube is fixed with a dripping head, the dripping head is fixed on the dripping table, and the dripping table is fixed on the inner wall above the body.

[0013] In a possible design, the arrangement state of each of the dropper heads of the dropper station is the same as the arrangement state of each of the reaction chambers on the disposable sample plate.

[0014] In a possible design, the temperature control mechanism further includes a control system and a temperature sensor provided at each of the heating plates, and the temperature control mechanism controls the heating plates to heat according to feedback information from the temperature sensors.

[0015] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0016] 1. The present invention designs a detection analyzer that uses loop-mediated isothermal amplification technology to detect meat bacteria. Compared with traditional colony detection methods, this device has a greatly shortened detection cycle. For food with a short shelf life such as meat, it can effectively reduce the direct economic losses caused by the detection cycle.

[0017] 2. The present invention has a high degree of automation and simple operation through the mutual cooperation of the primer reagent storage module, the conversion table assembly, the measurement system, the sample drop module and the disposable sample plate. It can complete the detection and analysis of multiple different types of bacteria at one time, which greatly reduces the detection and operation difficulty of the detection personnel. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings described herein are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation of the embodiments of the present invention. In the drawings:

[0019] Figure 1 It is a structural schematic diagram of the present invention;

[0020] Figure 2 is a side view of the present invention;

[0021] Figure 3 For the present invention Figure 2 Sectional view at AA in the middle;

[0022] Figure 4 A schematic diagram of the structure of a conversion platform assembly in an embodiment of the present invention;

[0023] Figure 5 It is a structural schematic diagram of a disposable sample plate in a first state according to an embodiment of the present invention;

[0024] Figure 6 It is a structural schematic diagram of the second state of the disposable sample plate in an embodiment of the present invention;

[0025] Figure 7 is a top view of a disposable sample plate in an embodiment of the present invention;

[0026] Figure 8 It is a bottom view of the dripping platform in the embodiment of the present invention.

[0027] The reference numerals represent: 1-body, 2-operation display panel, 3-primer storage cover, 4-detection port cover, 5-primer reagent storage tube, 6-conduit, 7-drip table, 8-fixed support seat, 9-rotating disk, 901-fixed protrusion, 10-primer reagent cold storage room, 11-rotation control motor, 12-fluorescence meter, 13-air pump, 14-disposable sample plate, 1401-annular groove, 1402-hard conical wall, 1403-bottom soft film, 1404-reaction chamber, 15-air guide tube, 16-heating plate, 17-drip head. DETAILED DESCRIPTION

[0028] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments and drawings. The exemplary embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.

[0029] Examples, such as Figures 1 to 8 As shown, this embodiment includes a primer reagent storage module, a conversion table assembly, a measurement system, a sample dropping module and a disposable sample plate 14 arranged in a body 1, the conversion table assembly includes a rotation control mechanism, a rotating disk 9, a temperature control mechanism and an air pressure control mechanism, the rotating disk 9 is provided with a plurality of fixing protrusions 901 for fixing the disposable sample plate 14, the temperature control mechanism includes a heating plate 16, and the heating plate 16 is fixed at the fixing protrusion 901, the primer reagent storage module is connected to the sample dropping module, and the primer reagent in the primer reagent storage module is dropped into the disposable sample plate 14 through the sample dropping module for isothermal amplification reaction, and the measurement system includes a fluorescence meter 12, and after the isothermal amplification reaction in the disposable sample plate 14 is completed, the fluorescence meter 12 is used to read the test result in the disposable sample plate 14.

[0030] Advantageously, an operation display panel 2 is fixedly provided on the top of the body 1, a primer storage cover 3 and a detection port cover 4 are provided on one side of the body 1, a primer reagent storage module is provided below the primer storage cover 3, and reagents can be added to and removed from the primer reagent storage module after opening the primer storage cover 3, and the detection port cover 4 is a sample dropping port, and the sample liquid can be dropped at the detection port cover 4 after opening the detection port cover 4.

[0031] In this embodiment, the disposable sample plate 14 is provided with a plurality of reaction chambers 1404, each of which is used to drop different primer reagents to detect different target bacteria, and the reaction chamber 1404 is surrounded by a hard conical wall 1402 and a bottom soft film 1403. The disposable sample plate 14 also includes an annular groove 1401 surrounding all the reaction chambers 1404. When the disposable sample plate 14 is aligned with the fixed protrusion 901 for installation, the fixed protrusion 901 is inserted into the bottom of the disposable sample plate 14, and the air pressure at the bottom of the disposable sample plate 14 increases, causing each bottom soft film 1403 to bulge upward (such as Figure 5 ), thereby, the volume of the reaction chamber 1404 is reduced. After the disposable sample plate 14 is fixed to the rotating disk 9, the sample liquid to be tested is directly aligned with the reaction chamber 1404 at the center position of the disposable sample plate 14 and added until each reaction chamber 1404 on the disposable sample plate 14 is filled with sample liquid, and the addition is stopped. The excess sample liquid is collected in the annular groove 1401. The above operation can automatically divide the sample liquid into multiple equal portions, which is simple and convenient.

[0032] Further, the air pressure control mechanism includes an air pump 13 and an air guide tube 15 provided in the rotating disk 9, one end of the air guide tube 15 is communicated with the internal cavity formed by the disposable sample plate 14 and the fixed protrusion 901, and the other end of the air guide tube 15 is communicated with the air inlet of the air pump 13. The rotating disk 9 is provided with the same number of the air guide tubes 15 as the fixed protrusion 901. After each reaction chamber 1404 in the disposable sample plate 14 is filled with sample liquid, the internal cavity formed by the disposable sample plate 14 and the fixed protrusion 901 is evacuated by the air pump 13. After evacuation, the shape of the disposable sample plate 14 is as follows: Figure 6 As shown, the reaction chamber 1404 is originally full, and after the bottom soft film 1403 is deformed, there is excess volume in the reaction chamber 1404. At this time, the rotating disk 9 can be controlled to rotate, and the rotating disk 9 rotates the disposable sample plate 14 to the next position for dripping the primer reagent.

[0033] In this embodiment, the rotation control mechanism includes a fixed support seat 8 fixed in the body 1 and a rotation control motor 11 fixed on the fixed support seat 8. The motor shaft of the rotation control motor 11 is fixed to the center position of the rotating disk 9. The fixed support seat 8 rotates in coordination with the rotating disk 9. The rotation of the rotating disk 9 can be controlled by the rotation control motor 11, and the rotation angle can be controlled.

[0034] In this embodiment, the primer reagent storage module includes a primer reagent cold storage room 10 and a primer reagent storage tube 5. A plurality of primer reagent storage tubes 5 are fixed in the primer reagent cold storage room 10. Different primer reagents are stored in each primer reagent storage tube 5. The primer reagent cold storage room 10 is used to store the primer reagents in the primer reagent storage tubes 5 at low temperatures, thereby ensuring the effectiveness of the primer reagents.

[0035] Furthermore, the sample dripping module includes a plurality of conduits 6, one end of each of the conduits 6 is connected to the bottom of a primer reagent storage tube 5, and the other end of the conduit 6 is fixed with a dripping head 17, and the dripping head 17 is fixed on the dripping platform 7, and the dripping platform 7 is fixed on the inner wall position above the body 1, so that the height of the dripping head 17 is higher than the highest position of the primer reagent storage tube 5. Optionally, the primer reagent cold storage chamber 10 is in a closed state after being closed in the primer storage cover 3. In this state, the primer reagent cold storage chamber 10 is in a closed state through the air pump 13 to the primer reagent storage tube 5. The ventilation in the primer reagent cold storage chamber 10 can use air pressure to press out the primer reagent in each primer reagent storage tube 5, thereby dripping the sample. The arrangement state of each dropper head 17 on the dropper table 7 is the same as the arrangement state of each reaction chamber 1404 on the disposable sample plate 14. After the disposable sample plate 14 is aligned with the dropper table 7, each dropper head 17 is automatically aligned with the reaction chamber 1404. At this time, it is only necessary to pressurize the primer reagent storage tube 5 to drip the primer reagent into each reaction chamber 1404 at one time.

[0036] Advantageously, the temperature control mechanism further comprises a control system and a temperature sensor provided at each of the heating plates 16. The temperature control mechanism controls the heating plates 16 to heat according to feedback information from the temperature sensors. In the present embodiment, the heating plates 16 are used for heating, and the fixed protrusions 901 and the disposable sample plates 14 are used to insulate the heating area. Compared with the conventional incubator, the present embodiment consumes significantly less energy and is more energy-efficient during isothermal amplification. After the primer reagent is dripped into the disposable sample plate 14, the heating can be turned on for isothermal amplification. During heating, the temperature of the disposable sample plate 14 and the inner chamber of the fixed protrusion 901 is maintained at 60 to 65 degrees Celsius. The heating can be performed for 40 to 60 minutes.

[0037] During the heating process, the rotating disk 9 can rotate freely to ensure that the disposable sample plates 14 at other positions complete the corresponding operations, so as to realize the inspection of multiple samples at the same time. When the heating time of one of the disposable sample plates 14 is over, the disposable sample plate 14 is controlled by the rotating disk 9 to rotate to the bottom of the fluorescence measuring instrument 12. The fluorescence measuring instrument 12 automatically reads the reaction results on the disposable sample plate 14 and displays the reaction results in the form of a report on the operation display panel 2. The whole device has a high degree of automation and a fast detection speed, and can greatly reduce the detection result errors caused by human operation factors.

[0038] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An analyzer for meat microbial detection, comprising a primer reagent storage module, a conversion table assembly, a measurement system, a sample dropping module and a disposable sample plate (14) arranged in a body (1), characterized in that: The conversion table assembly comprises a rotation control mechanism, a rotating disk (9), a temperature control mechanism and an air pressure control mechanism. The rotating disk (9) is provided with a plurality of fixing protrusions (901) for fixing the disposable sample plate (14). The temperature control mechanism comprises a heating plate (16). The heating plate (16) is fixedly arranged at the fixing protrusion (901). The primer reagent storage module is connected to the sample drop module. The measuring system comprises a fluorescence measuring instrument (12). After the isothermal amplification reaction in the disposable sample plate (14) is completed, the fluorescence measuring instrument (12) is used to read the test result in the disposable sample plate (14).

2. The analyzer for meat microbial detection according to claim 1, characterized in that: An operation display panel (2) is fixedly provided on the top of the machine body (1), a primer storage cover (3) and a detection port cover (4) are provided on one side of the machine body (1), a primer reagent storage module is provided below the primer storage cover (3), and a sample drop port is provided at the detection port cover (4).

3. The analyzer for meat microbial detection according to claim 1, characterized in that: The disposable sample plate (14) is provided with a plurality of reaction chambers (1404), each of the reaction chambers (1404) is used for dripping different primer reagents to detect different target bacteria, the reaction chamber (1404) is formed by a hard conical wall (1402) and a bottom soft film (1403), and the disposable sample plate (14) also includes an annular groove (1401) surrounding all the reaction chambers (1404).

4. The analyzer for meat microbial detection according to claim 1, characterized in that: The air pressure control mechanism comprises an air pump (13) and an air guide tube (15) arranged in the rotating disk (9), one end of the air guide tube (15) is connected to the internal cavity formed by the disposable sample plate (14) and the fixed protrusion (901), and the other end of the air guide tube (15) is connected to the air inlet of the air pump (13), and the rotating disk (9) is provided with the same number of air guide tubes (15) as the fixed protrusion (901).

5. The analyzer for meat microbial detection according to claim 1, characterized in that: The rotation control mechanism comprises a fixed support seat (8) fixed in the machine body (1) and a rotation control motor (11) fixed on the fixed support seat (8); the motor shaft of the rotation control motor (11) is fixed to the center position of the rotating disk (9); and the fixed support seat (8) and the rotating disk (9) are rotationally matched.

6. The analyzer for meat microbial detection according to claim 1, characterized in that: The primer reagent storage module comprises a primer reagent cold storage chamber (10) and a primer reagent storage tube (5). A plurality of primer reagent storage tubes (5) are fixed in the primer reagent cold storage chamber (10), each of the primer reagent storage tubes (5) storing a different primer reagent. The primer reagent cold storage chamber (10) is used to store the primer reagents in the primer reagent storage tubes (5) at low temperatures.

7. An analyzer for meat microbial detection according to claim 6, characterized in that: The sample dropping module comprises a plurality of conduits (6), one end of each of the conduits (6) being connected to the bottom of a primer reagent storage tube (5), and the other end of the conduit (6) being fixed with a dropper head (17), the dropper head (17) being fixed on a dropper table (7), and the dropper table (7) being fixed on the inner wall above the body (1).

8. The analyzer for meat microbial detection according to claim 7, characterized in that: The arrangement state of each of the dropper heads (17) of the dropper table (7) is the same as the arrangement state of each reaction chamber (1404) on the disposable sample plate (14).

9. The analyzer for meat microbial detection according to claim 1, characterized in that: The temperature control mechanism further comprises a control system and a temperature sensor provided at each of the heating plates (16), and the temperature control mechanism controls the heating plates (16) to heat according to feedback information from the temperature sensor.

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