Constant-temperature card box with nucleic acid molecule amplification structure and method thereof

Abstract

The invention relates to the technical field of amplification constant-temperature card boxes, and discloses a constant-temperature card box with a nucleic acid molecule amplification structure and a method thereof.The constant-temperature card box comprises a card box body, the interior of the card box body is hollow, a magnet is arranged on one side of the top of the card box body, a stable structure is arranged in the card box body, and constant-temperature structures are arranged on the two sides of the bottom of the card box body; the constant-temperature card box with the nucleic acid molecule amplification structure and the method have the advantages that the constant-temperature card box with the nucleic acid molecule amplification structure is provided with the heat preservation shell, and the heat preservation shell capable of carrying out temperature increasing, temperature decreasing and cooling treatment on amplification test tubes in a surrounding manner is arranged in the card box; the thermal insulation shell can perform all-directional temperature control on the single body to make the temperature more accurate, and can also be matched with the outer ring and the inner ring to perform balance control on the amplification test tube, so that the test tube is always in a vertical state when the clamping box moves and shakes, internal stock solution does not shake, and aerosol pollution is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of amplification thermostatic cartridges, and in particular to a thermostatic cartridge with a nucleic acid molecule amplification structure and a method thereof. Background Art

[0002] In the patent application with application publication number CN211365618U, it includes a box body and a top cover, wherein the top cover is arranged on the top of the box body, a front cover is hingedly connected to the bottom of the front end of the box body, and a plurality of fixing parts and supporting parts are symmetrically formed on both sides of the box body from top to bottom, each fixing part is respectively surrounded by the supporting part adjacent thereto to form a slot, and a buffer layer is provided on the inner surface of each slot, and a plurality of tube plates for placing reagent tubes are arranged in the box body from top to bottom, and the two sides of each tube plate are respectively inserted into the corresponding slots on both sides, and each tube plate is provided with a plurality of tube holes, and each tube hole is embedded with an elastic rubber ring, and the front cover The cover is arranged on the front end of the box body, and the rear end face of the front cover is against the front end face of each tube plate. The box body is integrally injection-molded by glass fiber reinforced PC composite plastic. The advantages are: the box body is integrally injection-molded by glass fiber reinforced PC composite plastic, has high strength, is not easy to deform when subjected to external force, and can well support and protect the reagent tubes in the box body; when the reagent box is shaken by the outside world, the elastic rubber ring sandwiched between each tube placement hole and the corresponding reagent tube of the utility model and the buffer layer on the inner surface of the slot can well mitigate the shaking effect of the outside world, can avoid collision of the reagent tubes in the box body, and thus can better protect the reagent tubes.

[0003] In the prior art including the above-mentioned patents, the card box in the PCR instrument cannot be effectively temperature controlled. Heating, heating and cooling too fast or too slow will affect the final experimental results. For example, the water-soluble type will cause the temperature to dissipate too quickly, and if the box body shakes after the amplification is completed, the liquid in the test tube will fluctuate greatly, which will cause more contact with the air and cause aerosol contamination, further affecting the accuracy of the experimental data. Summary of the invention

[0004] The problem to be solved by the present invention is that the heating and cooling of the amplification cartridge during use cannot be ideally controlled, which affects the accuracy of the experimental results and is prone to aerosol contamination.

[0005] In order to solve the above technical problems, the technical solution of the present invention is: a constant temperature cartridge with a nucleic acid molecule amplification structure and a method thereof, comprising a cartridge, the interior of the cartridge is hollow, a magnet is disposed on one side of the top of the cartridge, a stabilizing structure is disposed inside the cartridge, both sides of the bottom of the cartridge are provided with a constant temperature structure, the stabilizing structure comprises a placement plate, the placement plate is larger than the slot at the top of the cartridge, a plurality of square slots are disposed on the placement plate in a rectangular array, an outer ring is rotatably disposed between the two sides of the square slot, an inner ring is rotatably disposed between the front and back sides of the inner wall of the outer ring, and an amplification test tube is disposed inside the inner ring; An insulation shell is provided at the bottom of the inner ring. The insulation shell is open and its internal shape fits the external shape of the amplification test tube. The inner wall of the insulation shell is hollow. A spiral tube is provided inside the inner wall of the insulation shell. Telescopic tubes are passed through the top of the spiral tube on all sides.

[0006] Preferably, a partition plate is provided at the bottom of the inner wall of the card box, and the constant temperature structure includes a heater and a refrigerator, and the bottoms of the heater and the refrigerator are respectively fixedly connected to one side of the top of the partition plate.

[0007] Preferably, one side of the heating machine and the refrigerator are both fixedly connected with a connecting pipe, the connecting pipe is L-shaped, and a heating plate and a cooling plate are respectively provided on the top of the two connecting pipes.

[0008] Preferably, a water tank is fixedly provided on one side of the heating plate and the cooling plate, the number of the water tanks is four, every two water tanks form a group and an interconnecting pipe is fixedly connected between the opposite sides, one side of the water tank is fixedly connected to one side of the inner wall of the card box, and the top of one side of the water tank is fixedly connected to the telescopic tube.

[0009] Preferably, cylinders are provided on both sides of the top of the partition plate, and pneumatic telescopic rods are provided on the front and rear sides of the bottom of the inner wall of the card box. The top of the pneumatic telescopic rod passes through the water tank and extends to the interior of the water tank, and a sealing plate is provided on the top of the pneumatic telescopic rod.

[0010] Preferably, a rotating shaft is rotatably provided on one side of the inner wall of the water tank, a baffle is fixedly provided on one side of the rotating shaft, the baffle is circular, a secondary rod is rotatably provided on the rear side of one side of the baffle, a main rod is rotatably provided at the bottom of the secondary rod, and the bottom of the main rod is rotatably connected to one side of the top of the sealing plate.

[0011] Preferably, a cover is rotatably provided on one side of the top of the card box, a handle is provided on one side of the top of the cover, the handle is compatible with the magnet, a plurality of springs are provided on the top of the cover, a pressing plate is provided on the top of the spring, and limit blocks are provided on the front and rear sides of one side of the top of the card box.

[0012] Preferably, a plurality of cushions are provided on the top of the pressing plate, a plurality of circular center grooves are provided on the bottom of the cover plate, and a plurality of brackets are provided on the bottom of the pressing plate.

[0013] Preferably, the bottom of the bracket passes through the cover plate and extends to the outside of the cover plate, and a stabilizing plate is provided at the bottom of the bracket, and the stabilizing plate is matched with the circular groove.

[0014] S1: When in use, put the stock solution to be amplified into the amplification test tube, cover the test tube cap after putting it in, and then put the amplification test tube into the insulation shell, and then put the entire card box into the PCR device. After putting it in, the machine starts to operate heating, cooling and cooling. When heating, start the heating machine first, and the heating machine heats the heating plate through the connecting pipe. Then the heating plate will transfer the temperature to the water tank to increase the temperature of the liquid in the water tank; S2: After the liquid inside the water tank reaches the specified temperature, the cylinder is started. The cylinder will add air to the pneumatic telescopic rod to extend the pneumatic telescopic rod upward, and the pneumatic telescopic rod will drive the sealing plate to move upward. The sealing plate will also drive the main rod to move upward. The main rod controls the sub-rod to move upward and rotate the baffle with the rotating connection with the rotating shaft as the center to open. After opening, the liquid in the water tank will flow from the water tank into the telescopic tube and then flow along the telescopic tube to the spiral tube inside each insulation shell. The spiral tube is wrapped inside the insulation shell, and can perform all-round wrapped temperature increase on the amplification test tube. S3: As more liquid is injected, the main rod will be continuously pressurized and will fold around the rotation connection with the auxiliary rod, which will not affect the upward movement of the sealing plate. When the temperature is reduced after the heating, the cylinder can be controlled to recover the gas to allow the pneumatic telescopic rod to drive the sealing plate to move downward to draw the high-temperature liquid in the spiral tube back into the water tank. After the liquid is drawn back, as the main rod and the auxiliary rod are gradually straightened, the baffle rotates and closes again. When the cooling process is required after the cooling process is completed, the cylinder on the other side is started according to the above method to discharge the liquid cooled by the refrigeration plate from the water tank on the other side into the spiral tube for cooling. S4: After the steps of heating, cooling and deheating are completed, the cartridge is taken out of the device after the amplification of the stock solution is completed. If shaking occurs during the removal, the stock solution will sway, increase the contact between the stock solution and the air, and form aerosol pollution, which will affect the subsequent observation of the stock solution. When the cartridge is shaken after being taken out, the weight of the insulation shell is heavy, which will drive the amplification test tube inside it to always be in a vertical state. When subjected to longitudinal or lateral shaking force, the inner and outer rings on the top will rotate accordingly. During the rotation, the insulation shell and the amplification test tube will not change the vertical state. In this way, the stock solution can still be kept stable under shaking to reduce the occurrence of aerosol pollution. The insulation shell can be shaken without affecting its normal heating, cooling and deheating steps, so that the two can reach a better balance. If the user does not need it to remain in a stable state and needs to shake it manually to make the stock solution sway, such as adding liquid to allow it to be fully mixed with the stock solution, the user only needs to hold the cartridge in his hand and apply force downward on the pressing plate, and the pressing plate will drive the stabilizing plate to move downward through the bracket to fix the inner and outer rings.

[0015] Compared with the prior art, the technical solution of the present invention has the following advantages: (1) The cartridge is equipped with an insulation shell that can surround the amplification tube for heating, cooling and cooling. In addition to being able to perform all-round temperature control on the single unit to make the temperature more accurate, the insulation shell can also cooperate with the outer ring and the inner ring to balance the amplification tube, so that when the cartridge is moved and shaken, the amplification tube is always in a vertical state and the original liquid inside does not shake, thereby avoiding aerosol pollution. The insulation shell controls the liquid discharge and withdrawal by moving the sealing plate up and down in the water tank, and controls the baffle to open and close through its movement linkage, so that the liquid is opened when it is discharged and closed when it is returned. The insulation shell wraps each amplification tube separately, and has a certain temperature preservation effect to avoid temperature loss, which can make the temperature control more accurate and facilitate the amplification of the original liquid; (2) The insulation shell can achieve the balancing effect without affecting the temperature control of liquid injection, and the balancing effect can also be maintained manually. When it is not necessary to maintain the balance, such as when the original liquid needs to be fully stirred after adding the solution, the stabilizing plate can be used to limit the outer ring and the inner ring so that the outer ring and the inner ring no longer rotate. In this way, the amplification tube can be shaken to fully integrate the internal liquid, thereby increasing the overall controllability and adjustability of the device. The control method of the stabilizing plate is also simple and convenient, and it can be operated by pressing the finger. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the card box of the present invention; Figure 3It is a front view schematic diagram of the internal structure of the card box of the present invention; Figure 4 It is a schematic diagram of the stable structure of the present invention; Figure 5 This is a schematic diagram of the outer ring structure of the present invention; Figure 6 This is a schematic diagram of the inner ring structure of the present invention; Figure 7 This is an exploded schematic diagram of the spiral tube structure of the present invention; Figure 8 It is a schematic diagram of the constant temperature structure of the present invention; Fig. 9 This is a schematic diagram of the sealing plate structure of the present invention; Fig.10 This is a schematic diagram of the baffle structure of the present invention; Fig.11 It is a schematic diagram of the structure of the movable cover of the present invention; Fig.12 This is a schematic diagram of the structure of the stabilizing plate of the present invention; Fig.13 It is a schematic diagram of the external structure of the card box of the present invention.

[0017] In the figure: 1. cover plate; 2. pressing plate; 3. cushion; 4. magnet; 5. card box; 6. stable structure; 601. telescopic tube; 602. insulation shell; 603. inner ring; 604. outer ring; 605. placement plate; 606. amplification test tube; 607. spiral tube; 7. constant temperature structure; 701. water tank; 702. refrigerator; 703. connecting pipe; 704. pneumatic telescopic rod; 705. heating machine; 706. cylinder; 707. interconnecting pipe; 708. heating plate; 709. auxiliary rod; 710. main rod; 711. sealing plate; 712. rotating shaft; 713. baffle; 8. partition plate; 9. handle; 10. spring; 11. bracket; 12. stabilizing plate; 13. limit block. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0019] Unless otherwise defined, the technical terms or scientific terms used in the present disclosure should be understood by people with ordinary skills in the field to which the present disclosure belongs. "Include" or "comprise" and other similar words used in the present disclosure mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and other similar words are not limited to physical or mechanical connections, but may also include electrical connections, whether direct or indirect. "Up", "down", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0020] like Figures 1 to 13 As shown, the present invention provides a constant temperature cartridge with a nucleic acid molecule amplification structure and a method thereof, comprising: the interior of the cartridge 5 is hollow, a magnet 4 is provided on one side of the top of the cartridge 5, a stabilizing structure 6 is provided inside the cartridge 5, both sides of the bottom of the cartridge 5 are provided with a constant temperature structure 7, the stabilizing structure 6 comprises a placement plate 605, the placement plate 605 is larger than the slot at the top of the cartridge 5, a plurality of square slots are provided in a rectangular array on the placement plate 605, an outer ring 604 is rotatably provided between the two sides of the square slot, an inner ring 603 is rotatably provided between the front and back sides of the inner wall of the outer ring 604, and an amplification test tube 606 is provided in the inner ring 603; An insulation shell 602 is provided at the bottom of the inner ring 603. The insulation shell 602 is open and its internal shape fits the external shape of the amplification test tube 606. The inner wall of the insulation shell 602 is hollow. A spiral tube 607 is provided inside the inner wall of the insulation shell 602. Telescopic tubes 601 are passed through the top of the spiral tube 607 on all sides.

[0021] A partition plate 8 is provided at the bottom of the inner wall of the card box 5 , and the constant temperature structure 7 includes a heater 705 and a refrigerator 702 , and the bottoms of the heater 705 and the refrigerator 702 are fixedly connected to one side of the top of the partition plate 8 , respectively.

[0022] One side of the heating machine 705 and the refrigerator 702 are both fixedly connected with a connecting pipe 703, the connecting pipe 703 is L-shaped, and a heating plate 708 and a cooling plate are respectively disposed on the top of the two connecting pipes 703.

[0023] A water tank 701 is fixedly provided on one side of the heating plate 708 and the cooling plate. There are four water tanks 701, and every two water tanks 701 form a group. An interconnecting pipe 707 is fixedly connected between the opposite sides. One side of the water tank 701 is fixedly connected to one side of the inner wall of the card box 5, and the top of one side of the water tank 701 is fixedly connected to the telescopic tube 601.

[0024] Cylinders 706 are also provided on both sides of the top of the partition plate 8, and pneumatic telescopic rods 704 are provided on the front and rear sides of the bottom of the inner wall of the card box 5. The top of the pneumatic telescopic rod 704 penetrates the water tank 701 and extends to the inside of the water tank 701. A sealing plate 711 is provided on the top of the pneumatic telescopic rod 704.

[0025] A rotating shaft 712 is rotatably provided on one side of the inner wall of the water tank 701, and a baffle 713 is fixedly provided on one side of the rotating shaft 712. The baffle 713 is circular, and a secondary rod 709 is rotatably provided on the rear side of one side of the baffle 713. A main rod 710 is rotatably provided at the bottom of the secondary rod 709, and the bottom of the main rod 710 is rotatably connected to one side of the top of the sealing plate 711.

[0026] A cover plate 1 is rotatably provided on one side of the top of the card box 5, a handle 9 is provided on one side of the top of the cover plate 1, the handle 9 is adapted to the magnet 4, a plurality of springs 10 are provided on the top of the cover plate 1, a pressing plate 2 is provided on the top of the spring 10, and limit blocks 13 are provided on the front and rear sides of one side of the top of the card box 5. The cover plate 1 can be opened and closed by depressing the handle 9 when rotating to open, and the magnet 4 can fix the cover plate 1 when it is closed, so that the cover plate 1 will not loosen and open due to shaking or the like in the case of non-human operation.

[0027] A plurality of cushions 3 are disposed on the top of the pressing plate 2 , a plurality of circular center grooves are opened on the bottom of the cover plate 1 , and a plurality of brackets 11 are disposed on the bottom of the pressing plate 2 .

[0028] The bottom of the bracket 11 passes through the cover plate 1 and extends to the outside of the cover plate 1 . A stabilizing plate 12 is provided at the bottom of the bracket 11 , and the stabilizing plate 12 is matched with the circular groove.

[0029] S1: When in use, the stock solution to be amplified is placed into the amplification test tube 606, and the test tube cover is covered after being placed in, and then the amplification test tube 606 is placed in the heat preservation shell 602, and the card box 5 is placed in the PCR device after being placed in, and the machine starts to operate heating, cooling and cooling. When heating, the heating machine 705 is started first, and the heating machine 705 heats the heating plate 708 through the connecting pipe 703, and then the heating plate 708 transmits the temperature to the water tank 701 to increase the temperature of the liquid in the water tank 701; S2: After the liquid in the water tank 701 reaches the specified temperature, the cylinder 706 is started, and the cylinder 706 will add air to the pneumatic telescopic rod 704 to make the pneumatic telescopic rod 704 extend upward, and the pneumatic telescopic rod 704 will drive the sealing plate 711 to move upward. When the sealing plate 711 moves upward, it will drive the main rod 710 to move upward. The main rod 710 controls the sub-rod 709 to move upward to rotate the baffle 713 around the rotation connection with the rotating shaft 712 as the center of the circle to open. After opening, the liquid in the water tank 701 will enter the telescopic tube 601 from the water tank 701 and then flow along the telescopic tube 601 to the spiral tube 607 inside each insulation shell 602. The spiral tube 607 is wrapped inside the insulation shell 602, and can perform all-round wrapped temperature increase on the amplification test tube 606; S3: As more liquid is injected, the main rod 710 will be continuously pressurized and will fold with the rotation connection with the auxiliary rod 709 as the center, which will not affect the upward movement of the sealing plate 711. When the temperature is reduced after the heating is completed, the cylinder 706 can be controlled to recover the gas to allow the pneumatic telescopic rod 704 to drive the sealing plate 711 to move downward to draw the high-temperature liquid in the spiral tube 607 back into the water tank 701. After the liquid is drawn back, as the main rod 710 and the auxiliary rod 709 are gradually straightened, the baffle 713 is rotated and closed again. When the cooling process is required after the cooling is completed, the cylinder 706 on the other side is started according to the above method to discharge the liquid cooled by the refrigeration plate from the water tank 701 on the other side into the spiral tube 607 for cooling. S4: After the steps of heating, cooling and deheating are completed, the cartridge 5 is taken out of the device after the stock solution amplification is completed. If shaking occurs during the removal, the stock solution will sway and increase its contact with the air, forming aerosol pollution that affects the subsequent observation of the stock solution. When the cartridge 5 is shaken after being taken out, the heavy weight of the insulation shell 602 will drive the amplification test tube 606 inside it to always be in a vertical state. When subjected to longitudinal or lateral shaking force, the inner ring 603 and the outer ring 604 on the top will rotate accordingly. During the rotation, the insulation shell 602 and the amplification test tube 606 will not change their vertical state. state, so that the original liquid can still be kept stable when shaken to reduce the occurrence of aerosol pollution, and the insulation shell 602 can be shaken without affecting its normal heating, cooling and cooling steps, so that the two can reach a better balance. If the user does not need it to remain in a stable state and needs to shake it manually to make the original liquid sway, such as adding liquid to allow it to be fully mixed with the original liquid, at this time, the user only needs to hold the card box 5 in his hand and push down on the pressing plate 2, and the pressing plate 2 will drive the stabilizing plate 12 to move downward through the bracket 11 to fix the inner ring 603 and the outer ring 604.

[0030] The working principle and use process of the present invention are as follows: when in use, the stock solution to be amplified is placed into the amplification test tube 606, and the test tube cover is covered after being placed in, and then the amplification test tube 606 is placed in the heat preservation shell 602, and the card box 5 is placed in the PCR device after being placed in, and the machine starts to operate heating, cooling and cooling after being placed in, and the heating machine 705 is started first during heating, and the heating machine 705 heats the heating plate 708 through the connecting pipe 703, and then the heating plate 708 will conduct the temperature to the water tank 701 to increase the temperature of the liquid in the water tank 701, and the cylinder 706 is started after the liquid in the water tank 701 reaches the specified temperature, and the cylinder 706 will add air to the pneumatic telescopic rod 704 to make the pneumatic telescopic rod 704 extend upward, and the air The movable telescopic rod 704 will drive the sealing plate 711 to move upward. The sealing plate 711 will drive the main rod 710 to move upward. The main rod 710 controls the sub-rod 709 to move upward to rotate and open the baffle 713 with the rotating connection with the rotating shaft 712 as the center. After opening, the liquid in the water tank 701 will enter the telescopic tube 601 from the water tank 701 and then flow along the telescopic tube 601 to the spiral tube 607 inside each insulation shell 602. The spiral tube 607 is wrapped in the insulation shell 602 to perform all-round wrapping and temperature increase on the amplification test tube 606. As more liquid is injected, the main rod 710 will be continuously pressurized and will fold with the rotating connection with the sub-rod 709 as the center, which will not affect the upward movement of the sealing plate 711. After the temperature increase is completed, the temperature will be cooled. At the same time, the cylinder 706 can be controlled to recycle the gas so that the pneumatic telescopic rod 704 drives the sealing plate 711 to move downward to draw the high-temperature liquid in the spiral tube 607 back into the water tank 701. After being drawn back, as the main rod 710 and the auxiliary rod 709 are gradually straightened, the baffle 713 rotates and closes again. When the cooling is completed and cooling treatment is required, the cylinder 706 on the other side is started according to the above method. The liquid cooled by the refrigeration plate is discharged from the water tank 701 on the other side into the spiral tube 607 for cooling treatment. After the steps of heating, cooling and cooling are completed, the stock liquid amplification is completed and the card box 5 is taken out of the device. If shaking occurs during taking it out, it will cause the stock liquid to slosh and increase the contact with the air to form aerosol pollution, which affects the subsequent observation of the stock liquid. Shaking occurs after the card box 5 is taken out When the heat preservation shell 602 is swung, the heavy weight will drive the amplification test tube 606 inside it to always be in a vertical state. When subjected to longitudinal or lateral shaking force, the inner ring 603 and the outer ring 604 on the top will rotate accordingly. During the rotation, the heat preservation shell 602 and the amplification test tube 606 will not change the vertical state. In this way, the original liquid can still be kept stable under shaking to reduce the occurrence of aerosol pollution. The heat preservation shell 602 can be shaken without affecting its normal heating, cooling and cooling steps, so that the two can reach a better balance state. If the user does not need it to maintain a stable state and needs to shake the original liquid manually to make it sway, such as adding liquid to allow it to be fully mixed with the original liquid, the user only needs to hold the card box 5 in his hand and press the pressing plate 2 downward.The pressing plate 2 will drive the stabilizing plate 12 to move downward through the bracket 11 to fix the inner ring 603 and the outer ring 604 so that they no longer form a stabilizing effect, so that the original liquid in the amplification test tube 606 can be shaken.

[0031] The above embodiments are only exemplary embodiments of the present invention and are not intended to limit the present invention. The protection scope of the present invention is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the essence and protection scope of the present invention, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present invention.

Claims

1. A constant temperature cartridge having a nucleic acid molecule amplification structure, comprising a cartridge (5), characterized in that: The interior of the card box (5) is hollow, a magnet (4) is provided on one side of the top of the card box (5), a stabilizing structure (6) is provided inside the card box (5), and both sides of the bottom of the card box (5) are provided with a constant temperature structure (7), the stabilizing structure (6) comprises a placement plate (605), the placement plate (605) is larger than the slot at the top of the card box (5), a plurality of square slots are provided on the placement plate (605) in a rectangular array, an outer ring (604) is rotatably provided between the two sides of the square slot, an inner ring (603) is rotatably provided between the front and back sides of the inner wall of the outer ring (604), and an amplification test tube (606) is provided inside the inner ring (603); A heat-insulating shell (602) is provided at the bottom of the inner ring (603); the heat-insulating shell (602) is open and its internal shape fits the external shape of the amplification test tube (606); the inner wall of the heat-insulating shell (602) is hollow; a spiral tube (607) is provided inside the inner wall of the heat-insulating shell (602); and telescopic tubes (601) are passed through the four sides of the top of the spiral tube (607).

2. A constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 1, characterized in that: A partition plate (8) is provided at the bottom of the inner wall of the card box (5), and the constant temperature structure (7) comprises a heater (705) and a refrigerator (702), wherein the bottoms of the heater (705) and the refrigerator (702) are respectively fixedly connected to one side of the top of the partition plate (8).

3. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 2, characterized in that: One side of the heating machine (705) and the refrigerator (702) are both fixedly connected with a connecting pipe (703), the connecting pipe (703) is L-shaped, and a heating plate (708) and a cooling plate are respectively provided on the top of the two connecting pipes (703).

4. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 3, characterized in that: A water tank (701) is fixedly provided on one side of the heating plate (708) and the cooling plate. The number of the water tanks (701) is four. Every two water tanks (701) form a group and an interconnecting pipe (707) is fixedly connected between opposite sides. One side of the water tank (701) is fixedly connected to one side of the inner wall of the card box (5), and the top of one side of the water tank (701) is fixedly connected to the telescopic tube (601).

5. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 2, characterized in that: Cylinders (706) are also provided on both sides of the top of the partition plate (8), and pneumatic telescopic rods (704) are provided on the front and rear sides of both sides of the bottom of the inner wall of the card box (5), the top of the pneumatic telescopic rod (704) penetrates the water tank (701) and extends to the inside of the water tank (701), and a sealing plate (711) is provided on the top of the pneumatic telescopic rod (704).

6. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 4, characterized in that: A rotating shaft (712) is rotatably provided on one side of the inner wall of the water tank (701), a baffle (713) is fixedly provided on one side of the rotating shaft (712), the baffle (713) is circular, a secondary rod (709) is rotatably provided on the rear side of one side of the baffle (713), a main rod (710) is rotatably provided on the bottom of the secondary rod (709), and the bottom of the main rod (710) is rotatably connected to one side of the top of the sealing plate (711).

7. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 1, characterized in that: A cover plate (1) is rotatably provided on one side of the top of the card box (5); a handle (9) is provided on one side of the top of the cover plate (1); the handle (9) is compatible with the magnet (4); a plurality of springs (10) are provided on the top of the cover plate (1); a pressing plate (2) is provided on the top of the spring (10); and limit blocks (13) are provided on the front and rear sides of one side of the top of the card box (5).

8. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 7, characterized in that: The top of the pressing plate (2) is provided with a plurality of soft pads (3), the bottom of the cover plate (1) is provided with a plurality of circular grooves, and the bottom of the pressing plate (2) is provided with a plurality of brackets (11).

9. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 8, characterized in that: The bottom of the bracket (11) passes through the cover plate (1) and extends to the outside of the cover plate (1), and a stabilizing plate (12) is provided at the bottom of the bracket (11), and the stabilizing plate (12) is adapted to the circular groove.

10. The constant temperature cartridge with a nucleic acid molecule amplification structure according to claim 1, characterized in that: The operation process steps are as follows: S1: When in use, the stock solution to be amplified is placed into the amplification test tube (606), and the test tube cover is covered after being placed in, and then the amplification test tube (606) is placed in the heat preservation shell (602), and then the card box (5) is placed in the PCR device. After being placed in, the machine starts to operate heating, cooling and cooling. When heating, the heating machine (705) is started first, and the heating machine (705) heats the heating plate (708) through the connecting pipe (703), and then the heating plate (708) transmits the temperature to the water tank (701) to increase the temperature of the liquid in the water tank (701); S2: After the liquid inside the water tank (701) reaches a specified temperature, the cylinder (706) is started, and the cylinder (706) adds air to the pneumatic telescopic rod (704) to extend the pneumatic telescopic rod (704) upward, and the pneumatic telescopic rod (704) drives the sealing plate (711) to move upward. When the sealing plate (711) moves upward, it drives the main rod (710) to move upward. The main rod (710) controls the sub-rod (709) to move upward and rotates the baffle (713) around the rotation connection with the rotating shaft (712) to open it. After opening, the liquid in the water tank (701) enters the telescopic tube (601) from the water tank (701) and then flows along the telescopic tube (601) to the spiral tube (607) inside each insulation shell (602). The spiral tube (607) is wrapped inside the insulation shell (602) to increase the temperature of the amplification test tube (606) in an all-round manner; S3: As more liquid is injected, the main rod (710) will be continuously pressurized and will fold around the rotation connection with the auxiliary rod (709) as the center without affecting the upward movement of the sealing plate (711). When the temperature is reduced after the heating is completed, the cylinder (706) can be controlled to recover the gas so that the pneumatic telescopic rod (704) drives the sealing plate (711) to move downward to draw the high-temperature liquid in the spiral tube (607) back into the water tank (701). After the liquid is drawn back, as the main rod (710) and the auxiliary rod (709) are gradually straightened, the baffle (713) is rotated and closed again. When the temperature is reduced and cooling is required, the cylinder (706) on the other side is started according to the above method to discharge the liquid cooled by the refrigeration plate from the water tank (701) on the other side into the spiral tube (607) for cooling. S4: After the steps of heating, cooling and deheating are completed, the cartridge (5) is taken out of the device after the stock solution amplification is completed. If the cartridge (5) is shaken during removal, the stock solution will sway and increase its contact with the air, forming aerosol contamination and affecting subsequent observation of the stock solution. When the cartridge (5) is shaken after being taken out, the amplification test tube (606) inside it will always be in a vertical state due to the heavy weight of the insulation shell (602). When the inner ring (603) and the outer ring (604) on the top thereof are shaken longitudinally or transversely, they will rotate accordingly. During the rotation, the insulation shell (602) and the amplification test tube (606) will not change their vertical state. state, so that the original liquid can still be kept stable when it is shaken to reduce the occurrence of aerosol pollution. The insulation shell (602) can be shaken without affecting its normal heating, cooling and cooling steps, so that the two can reach a better balance state. If the user does not need it to remain in a stable state and needs to shake the original liquid manually to make it sway, such as adding liquid to allow it to be fully mixed with the original liquid, at this time, the user only needs to hold the card box (5) in his hand and apply force to the pressing plate (2) downward, and the pressing plate (2) will drive the stabilizing plate (12) to move downward through the bracket (11) to fix the inner ring (603) and the outer ring (604).

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